["[Dialogue between User and Assistant on 2024-01-01 00:00]\n<User>Here are some new facts I learnt just now:\nBertrand Russell saw the Enlightenment as a phase in a progressive development, which began in antiquity, and that reason and challenges to the established order were constant ideals throughout that time. Russell said that the Enlightenment was ultimately born out of the Protestant reaction against the Catholic counter-reformation, and that philosophical views such as affinity for democracy against monarchy originated among 16th-century Protestants to justify their desire to break away from the Catholic Church. Though many of these philosophical ideals were picked up by Catholics, Russell argues, by the 18th century the Enlightenment was the principal manifestation of the schism that began with Martin Luther.\n\nAlexis de Tocqueville described the French Revolution as the inevitable result of the radical opposition created in the 18th century between the monarchy and the men of letters of the Enlightenment. These men of letters constituted a sort of \"substitute aristocracy that was both all-powerful and without real power\". This illusory power came from the rise of \"public opinion\", born when absolutist centralization removed the nobility and the bourgeoisie from the political sphere. The \"literary politics\" that resulted promoted a discourse of equality and was hence in fundamental opposition to the monarchical regime. De Tocqueville \"clearly designates ... the cultural effects of transformation in the forms of the exercise of power\". Nevertheless, it took another century before cultural approach became central to the historiography, as typified by Robert Darnton, The Business of Enlightenment: A Publishing History of the Encyclop\u00e9die, 1775\u20131800 (1979).\n\nEnlightenment historiography began in the period itself, from what Enlightenment figures said about their work. A dominant element was the intellectual angle they took. D'Alembert's Preliminary Discourse of l'Encyclop\u00e9die provides a history of the Enlightenment which comprises a chronological list of developments in the realm of knowledge \u2013 of which the Encyclop\u00e9die forms the pinnacle. In 1783, Jewish philosopher Moses Mendelssohn referred to Enlightenment as a process by which man was educated in the use of reason. Immanuel Kant called Enlightenment \"man's release from his self-incurred tutelage\", tutelage being \"man's inability to make use of his understanding without direction from another\". \"For Kant, Enlightenment was mankind's final coming of age, the emancipation of the human consciousness from an immature state of ignorance.\" The German scholar Ernst Cassirer called the Enlightenment \"a part and a special phase of that whole intellectual development through which modern philosophic thought gained its characteristic self-confidence and self-consciousness\". According to historian Roy Porter, the liberation of the human mind from a dogmatic state of ignorance is the epitome of what the Age of Enlightenment was trying to capture.\n\nThese views on religious tolerance and the importance of individual conscience, along with the social contract, became particularly influential in the American colonies and the drafting of the United States Constitution. Thomas Jefferson called for a \"wall of separation between church and state\" at the federal level. He previously had supported successful efforts to disestablish the Church of England in Virginia, and authored the Virginia Statute for Religious Freedom. Jefferson's political ideals were greatly influenced by the writings of John Locke, Francis Bacon, and Isaac Newton whom he considered the three greatest men that ever lived.\n\nIn addition to debates on religion, societies discussed issues such as politics and the role of women. It is important to note, however, that the critical subject matter of these debates did not necessarily translate into opposition to the government. In other words, the results of the debate quite frequently upheld the status quo. From a historical standpoint, one of the most important features of the debating society was their openness to the public; women attended and even participated in almost every debating society, which were likewise open to all classes providing they could pay the entrance fee. Once inside, spectators were able to participate in a largely egalitarian form of sociability that helped spread Enlightenment ideas.\n\nIntellectuals such as Robert Darnton and J\u00fcrgen Habermas have focused on the social conditions of the Enlightenment. Habermas described the creation of the \"bourgeois public sphere\" in 18th-century Europe, containing the new venues and modes of communication allowing for rational exchange. Habermas said that the public sphere was bourgeois, egalitarian, rational, and independent from the state, making it the ideal venue for intellectuals to critically examine contemporary politics and society, away from the interference of established authority. While the public sphere is generally an integral component of the social study of the Enlightenment, other historians have questioned whether the public sphere had these characteristics.\n\nJonathan Israel rejects the attempts of postmodern and Marxian historians to understand the revolutionary ideas of the period purely as by-products of social and economic transformations. He instead focuses on the history of ideas in the period from 1650 to the end of the 18th century, and claims that it was the ideas themselves that caused the change that eventually led to the revolutions of the latter half of the 18th century and the early 19th century. Israel argues that until the 1650s Western civilization \"was based on a largely shared core of faith, tradition and authority\".\n\nOne of the primary elements of the culture of the Enlightenment was the rise of the public sphere, a \"realm of communication marked by new arenas of debate, more open and accessible forms of urban public space and sociability, and an explosion of print culture,\" in the late 17th century and 18th century. Elements of the public sphere included: it was egalitarian, it discussed the domain of \"common concern,\" and argument was founded on reason. Habermas uses the term \"common concern\" to describe those areas of political/social knowledge and discussion that were previously the exclusive territory of the state and religious authorities, now open to critical examination by the public sphere. The values of this bourgeois public sphere included holding reason to be supreme, considering everything to be open to criticism (the public sphere is critical), and the opposition of secrecy of all sorts.\n<Assistant>I've captured all of this valuable information.", "[Dialogue between User and Assistant on 2024-01-02 00:00]\n<User>I want to share some knowledge I just acquired:\nAcross continental Europe, but in France especially, booksellers and publishers had to negotiate censorship laws of varying strictness. The Encyclop\u00e9die, for example, narrowly escaped seizure and had to be saved by Malesherbes, the man in charge of the French censure. Indeed, many publishing companies were conveniently located outside France so as to avoid overzealous French censors. They would smuggle their merchandise across the border, where it would then be transported to clandestine booksellers or small-time peddlers. The records of clandestine booksellers may give a better representation of what literate Frenchmen might have truly read, since their clandestine nature provided a less restrictive product choice. In one case, political books were the most popular category, primarily libels and pamphlets. Readers were more interested in sensationalist stories about criminals and political corruption than they were in political theory itself. The second most popular category, \"general works\" (those books \"that did not have a dominant motif and that contained something to offend almost everyone in authority\") demonstrated a high demand for generally low-brow subversive literature. However, these works never became part of literary canon, and are largely forgotten today as a result.\n\nA healthy, and legal, publishing industry existed throughout Europe, although established publishers and book sellers occasionally ran afoul of the law. The Encyclop\u00e9die, for example, condemned not only by the King but also by Clement XII, nevertheless found its way into print with the help of the aforementioned Malesherbes and creative use of French censorship law. But many works were sold without running into any legal trouble at all. Borrowing records from libraries in England, Germany and North America indicate that more than 70 percent of books borrowed were novels. Less than 1 percent of the books were of a religious nature, indicating the general trend of declining religiosity.\n\nThe Republic of Letters was the sum of a number of Enlightenment ideals: an egalitarian realm governed by knowledge that could act across political boundaries and rival state power. It was a forum that supported \"free public examination of questions regarding religion or legislation\". Immanuel Kant considered written communication essential to his conception of the public sphere; once everyone was a part of the \"reading public\", then society could be said to be enlightened. The people who participated in the Republic of Letters, such as Diderot and Voltaire, are frequently known today as important Enlightenment figures. Indeed, the men who wrote Diderot's Encyclop\u00e9die arguably formed a microcosm of the larger \"republic\".\n<Assistant>Perfect. I've stored this information in my memory.", "[Dialogue between User and Assistant on 2024-01-03 00:00]\n<User>Let me share some additional facts I found:\nJonathan Israel called the journals the most influential cultural innovation of European intellectual culture. They shifted the attention of the \"cultivated public\" away from established authorities to novelty and innovation, and promoted the \"enlightened\" ideals of toleration and intellectual objectivity. Being a source of knowledge derived from science and reason, they were an implicit critique of existing notions of universal truth monopolized by monarchies, parliaments, and religious authorities. They also advanced Christian enlightenment that upheld \"the legitimacy of God-ordained authority\"\u2014the Bible\u2014in which there had to be agreement between the biblical and natural theories.\n\nAlthough the existence of dictionaries and encyclopedias spanned into ancient times, the texts changed from simply defining words in a long running list to far more detailed discussions of those words in 18th-century encyclopedic dictionaries. The works were part of an Enlightenment movement to systematize knowledge and provide education to a wider audience than the elite. As the 18th century progressed, the content of encyclopedias also changed according to readers' tastes. Volumes tended to focus more strongly on secular affairs, particularly science and technology, rather than matters of theology.\n\nThe massive work was arranged according to a \"tree of knowledge.\" The tree reflected the marked division between the arts and sciences, which was largely a result of the rise of empiricism. Both areas of knowledge were united by philosophy, or the trunk of the tree of knowledge. The Enlightenment's desacrilization of religion was pronounced in the tree's design, particularly where theology accounted for a peripheral branch, with black magic as a close neighbour. As the Encyclop\u00e9die gained popularity, it was published in quarto and octavo editions after 1777. The quarto and octavo editions were much less expensive than previous editions, making the Encyclop\u00e9die more accessible to the non-elite. Robert Darnton estimates that there were approximately 25 000 copies of the Encyclop\u00e9die in circulation throughout France and Europe before the French Revolution. The extensive, yet affordable encyclopedia came to represent the transmission of Enlightenment and scientific education to an expanding audience.\n\nIn England, the Royal Society of London also played a significant role in the public sphere and the spread of Enlightenment ideas. It was founded by a group of independent scientists and given a royal charter in 1662. The Society played a large role in spreading Robert Boyle's experimental philosophy around Europe, and acted as a clearinghouse for intellectual correspondence and exchange. Boyle was \"a founder of the experimental world in which scientists now live and operate,\" and his method based knowledge on experimentation, which had to be witnessed to provide proper empirical legitimacy. This is where the Royal Society came into play: witnessing had to be a \"collective act\", and the Royal Society's assembly rooms were ideal locations for relatively public demonstrations. However, not just any witness was considered to be credible; \"Oxford professors were accounted more reliable witnesses than Oxfordshire peasants.\" Two factors were taken into account: a witness's knowledge in the area; and a witness's \"moral constitution\". In other words, only civil society were considered for Boyle's public.\n\nThe debating societies discussed an extremely wide range of topics. Before the Enlightenment, most intellectual debates revolved around \"confessional\" \u2013 that is, Catholic, Lutheran, Reformed (Calvinist), or Anglican issues, and the main aim of these debates was to establish which bloc of faith ought to have the \"monopoly of truth and a God-given title to authority\". After this date everything thus previously rooted in tradition was questioned and often replaced by new concepts in the light of philosophical reason. After the second half of the 17th century and during the 18th century, a \"general process of rationalization and secularization set in,\" and confessional disputes were reduced to a secondary status in favor of the \"escalating contest between faith and incredulity\".\n<Assistant>I understand. I'll keep this in mind.", "[Dialogue between User and Assistant on 2024-01-04 00:00]\n<User>Let me update you with some new information:\nGerman historian Reinhart Koselleck claimed that \"On the Continent there were two social structures that left a decisive imprint on the Age of Enlightenment: the Republic of Letters and the Masonic lodges.\" Scottish professor Thomas Munck argues that \"although the Masons did promote international and cross-social contacts which were essentially non-religious and broadly in agreement with enlightened values, they can hardly be described as a major radical or reformist network in their own right.\" Many of the Masons values seemed to greatly appeal to Enlightenment values and thinkers. Diderot discusses the link between Freemason ideals and the enlightenment in D'Alembert's Dream, exploring masonry as a way of spreading enlightenment beliefs. Historian Margaret Jacob stresses the importance of the Masons in indirectly inspiring enlightened political thought. On the negative side, Daniel Roche contests claims that Masonry promoted egalitarianism. He argues that the lodges only attracted men of similar social backgrounds. The presence of noble women in the French \"lodges of adoption\" that formed in the 1780s was largely due to the close ties shared between these lodges and aristocratic society.\n<Assistant>Thanks for letting me know. I'll keep track of this.", "[Dialogue between User and Assistant on 2024-01-05 00:00]\n<User>Here are some news I learnt earlier:\nAlong with secular matters, readers also favoured an alphabetical ordering scheme over cumbersome works arranged along thematic lines. The historian Charles Porset, commenting on alphabetization, has said that \"as the zero degree of taxonomy, alphabetical order authorizes all reading strategies; in this respect it could be considered an emblem of the Enlightenment.\" For Porset, the avoidance of thematic and hierarchical systems thus allows free interpretation of the works and becomes an example of egalitarianism. Encyclopedias and dictionaries also became more popular during the Age of Reason as the number of educated consumers who could afford such texts began to multiply. In the later half of the 18th century, the number of dictionaries and encyclopedias published by decade increased from 63 between 1760 and 1769 to approximately 148 in the decade proceeding the French Revolution (1780\u20131789). Along with growth in numbers, dictionaries and encyclopedias also grew in length, often having multiple print runs that sometimes included in supplemented editions.\n\nOne of the most important developments that the Enlightenment era brought to the discipline of science was its popularization. An increasingly literate population seeking knowledge and education in both the arts and the sciences drove the expansion of print culture and the dissemination of scientific learning. The new literate population was due to a high rise in the availability of food. This enabled many people to rise out of poverty, and instead of paying more for food, they had money for education. Popularization was generally part of an overarching Enlightenment ideal that endeavoured \"to make information available to the greatest number of people.\" As public interest in natural philosophy grew during the 18th century, public lecture courses and the publication of popular texts opened up new roads to money and fame for amateurs and scientists who remained on the periphery of universities and academies. More formal works included explanations of scientific theories for individuals lacking the educational background to comprehend the original scientific text. Sir Isaac Newton's celebrated Philosophiae Naturalis Principia Mathematica was published in Latin and remained inaccessible to readers without education in the classics until Enlightenment writers began to translate and analyze the text in the vernacular.\n\nCircadian rhythms allow organisms to anticipate and prepare for precise and regular environmental changes. They thus enable organisms to best capitalize on environmental resources (e.g. light and food) compared to those that cannot predict such availability. It has therefore been suggested that circadian rhythms put organisms at a selective advantage in evolutionary terms. However, rhythmicity appears to be as important in regulating and coordinating internal metabolic processes, as in coordinating with the environment. This is suggested by the maintenance (heritability) of circadian rhythms in fruit flies after several hundred generations in constant laboratory conditions, as well as in creatures in constant darkness in the wild, and by the experimental elimination of behavioral, but not physiological, circadian rhythms in quail.\n\nNorwegian researchers at the University of Troms\u00f8 have shown that some Arctic animals (ptarmigan, reindeer) show circadian rhythms only in the parts of the year that have daily sunrises and sunsets. In one study of reindeer, animals at 70 degrees North showed circadian rhythms in the autumn, winter and spring, but not in the summer. Reindeer on Svalbard at 78 degrees North showed such rhythms only in autumn and spring. The researchers suspect that other Arctic animals as well may not show circadian rhythms in the constant light of summer and the constant dark of winter.\n\nThe central oscillator generates a self-sustaining rhythm and is driven by two interacting feedback loops that are active at different times of day. The morning loop consists of CCA1 (Circadian and Clock-Associated 1) and LHY (Late Elongated Hypocotyl), which encode closely related MYB transcription factors that regulate circadian rhythms in Arabidopsis, as well as PRR 7 and 9 (Pseudo-Response Regulators.) The evening loop consists of GI (Gigantea) and ELF4, both involved in regulation of flowering time genes. When CCA1 and LHY are overexpressed (under constant light or dark conditions), plants become arrhythmic, and mRNA signals reduce, contributing to a negative feedback loop. Gene expression of CCA1 and LHY oscillates and peaks in the early morning, whereas TOC1 gene expression oscillates and peaks in the early evening. While it was previously hypothesised that these three genes model a negative feedback loop in which over-expressed CCA1 and LHY repress TOC1 and over-expressed TOC1 is a positive regulator of CCA1 and LHY, it was shown in 2012 by Andrew Millar and others that TOC1 in fact serves as a repressor not only of CCA1, LHY, and PRR7 and 9 in the morning loop but also of GI and ELF4 in the evening loop. This finding and further computational modeling of TOC1 gene functions and interactions suggest a reframing of the plant circadian clock as a triple negative-component repressilator model rather than the positive/negative-element feedback loop characterizing the clock in mammals.\n\nA defect in the human homologue of the Drosophila \"period\" gene was identified as a cause of the sleep disorder FASPS (Familial advanced sleep phase syndrome), underscoring the conserved nature of the molecular circadian clock through evolution. Many more genetic components of the biological clock are now known. Their interactions result in an interlocked feedback loop of gene products resulting in periodic fluctuations that the cells of the body interpret as a specific time of the day.[citation needed]\n\nDue to the work nature of airline pilots, who often cross several timezones and regions of sunlight and darkness in one day, and spend many hours awake both day and night, they are often unable to maintain sleep patterns that correspond to the natural human circadian rhythm; this situation can easily lead to fatigue. The NTSB cites this as contributing to many accidents[unreliable medical source?]  and has conducted several research studies in order to find methods of combating fatigue in pilots.\n\nThe earliest recorded account of a circadian process dates from the 4th century B.C.E., when Androsthenes, a ship captain serving under Alexander the Great, described diurnal leaf movements of the tamarind tree. The observation of a circadian or diurnal process in humans is mentioned in Chinese medical texts dated to around the 13th century, including the Noon and Midnight Manual and the Mnemonic Rhyme to Aid in the Selection of Acu-points According to the Diurnal Cycle, the Day of the Month and the Season of the Year.\n\nPlant circadian rhythms tell the plant what season it is and when to flower for the best chance of attracting pollinators. Behaviors showing rhythms include leaf movement, growth, germination, stomatal/gas exchange, enzyme activity, photosynthetic activity, and fragrance emission, among others. Circadian rhythms occur as a plant entrains to synchronize with the light cycle of its surrounding environment. These rhythms are endogenously generated and self-sustaining and are relatively constant over a range of ambient temperatures. Important features include two interacting transcription-translation feedback loops: proteins containing PAS domains, which facilitate protein-protein interactions; and several photoreceptors that fine-tune the clock to different light conditions. Anticipation of changes in the environment allows appropriate changes in a plant's physiological state, conferring an adaptive advantage. A better understanding of plant circadian rhythms has applications in agriculture, such as helping farmers stagger crop harvests to extend crop availability and securing against massive losses due to weather.\n\nThe simplest known circadian clock is that of the prokaryotic cyanobacteria. Recent research has demonstrated that the circadian clock of Synechococcus elongatus can be reconstituted in vitro with just the three proteins (KaiA, KaiB, KaiC) of their central oscillator. This clock has been shown to sustain a 22-hour rhythm over several days upon the addition of ATP. Previous explanations of the prokaryotic circadian timekeeper were dependent upon a DNA transcription/translation feedback mechanism.[citation needed]\n\nThe rhythm is linked to the light\u2013dark cycle. Animals, including humans, kept in total darkness for extended periods eventually function with a free-running rhythm. Their sleep cycle is pushed back or forward each \"day\", depending on whether their \"day\", their endogenous period, is shorter or longer than 24 hours. The environmental cues that reset the rhythms each day are called zeitgebers (from the German, \"time-givers\"). Totally blind subterranean mammals (e.g., blind mole rat Spalax sp.) are able to maintain their endogenous clocks in the apparent absence of external stimuli. Although they lack image-forming eyes, their photoreceptors (which detect light) are still functional; they do surface periodically as well.[page needed]\n<Assistant>Thanks for the update. I've recorded these facts.", "[Dialogue between User and Assistant on 2024-01-06 00:00]\n<User>Here's some valuable information I collected:\nMelatonin is absent from the system or undetectably low during daytime. Its onset in dim light, dim-light melatonin onset (DLMO), at roughly 21:00 (9 p.m.) can be measured in the blood or the saliva. Its major metabolite can also be measured in morning urine. Both DLMO and the midpoint (in time) of the presence of the hormone in the blood or saliva have been used as circadian markers. However, newer research indicates that the melatonin offset may be the more reliable marker. Benloucif et al. found that melatonin phase markers were more stable and more highly correlated with the timing of sleep than the core temperature minimum. They found that both sleep offset and melatonin offset are more strongly correlated with phase markers than the onset of sleep. In addition, the declining phase of the melatonin levels is more reliable and stable than the termination of melatonin synthesis.\n\nFor temperature studies, subjects must remain awake but calm and semi-reclined in near darkness while their rectal temperatures are taken continuously. Though variation is great among normal chronotypes, the average human adult's temperature reaches its minimum at about 05:00 (5 a.m.), about two hours before habitual wake time. Baehr et al. found that, in young adults, the daily body temperature minimum occurred at about 04:00 (4 a.m.) for morning types but at about 06:00 (6 a.m.) for evening types. This minimum occurred at approximately the middle of the eight hour sleep period for morning types, but closer to waking in evening types.\n\nIn 1896, Patrick and Gilbert observed that during a prolonged period of sleep deprivation, sleepiness increases and decreases with a period of approximately 24 hours. In 1918, J.S. Szymanski showed that animals are capable of maintaining 24-hour activity patterns in the absence of external cues such as light and changes in temperature. In the early 20th century, circadian rhythms were noticed in the rhythmic feeding times of bees. Extensive experiments were done by Auguste Forel, Ingeborg Beling, and Oskar Wahl to see whether this rhythm was due to an endogenous clock.[citation needed] Ron Konopka and Seymour Benzer isolated the first clock mutant in Drosophila in the early 1970s and mapped the \"period\" gene, the first discovered genetic determinant of behavioral rhythmicity. Joseph Takahashi discovered the first mammalian circadian clock mutation (clock\u039419) using mice in 1994. However, recent studies show that deletion of clock does not lead to a behavioral phenotype (the animals still have normal circadian rhythms), which questions its importance in rhythm generation.\n\nIt is now known that the molecular circadian clock can function within a single cell; i.e., it is cell-autonomous. This was shown by Gene Block in isolated mollusk BRNs.[clarification needed] At the same time, different cells may communicate with each other resulting in a synchronised output of electrical signaling. These may interface with endocrine glands of the brain to result in periodic release of hormones. The receptors for these hormones may be located far across the body and synchronise the peripheral clocks of various organs. Thus, the information of the time of the day as relayed by the eyes travels to the clock in the brain, and, through that, clocks in the rest of the body may be synchronised. This is how the timing of, for example, sleep/wake, body temperature, thirst, and appetite are coordinately controlled by the biological clock.[citation needed]\n\nLight is the signal by which plants synchronize their internal clocks to their environment and is sensed by a wide variety of photoreceptors. Red and blue light are absorbed through several phytochromes and cryptochromes. One phytochrome, phyA, is the main phytochrome in seedlings grown in the dark but rapidly degrades in light to produce Cry1. Phytochromes B\u2013E are more stable with phyB, the main phytochrome in seedlings grown in the light. The cryptochrome (cry) gene is also a light-sensitive component of the circadian clock and is thought to be involved both as a photoreceptor and as part of the clock's endogenous pacemaker mechanism. Cryptochromes 1\u20132 (involved in blue\u2013UVA) help to maintain the period length in the clock through a whole range of light conditions.\n\nStudies by Nathaniel Kleitman in 1938 and by Derk-Jan Dijk and Charles Czeisler in the 1990s put human subjects on enforced 28-hour sleep\u2013wake cycles, in constant dim light and with other time cues suppressed, for over a month. Because normal people cannot entrain to a 28-hour day in dim light if at all,[citation needed] this is referred to as a forced desynchrony protocol. Sleep and wake episodes are uncoupled from the endogenous circadian period of about 24.18 hours and researchers are allowed to assess the effects of circadian phase on aspects of sleep and wakefulness including sleep latency and other functions.[page needed]\n\nShift-work or chronic jet-lag have profound consequences on circadian and metabolic events in the body. Animals that are forced to eat during their resting period show increased body mass and altered expression of clock and metabolic genes.[medical citation needed] In humans, shift-work that favors irregular eating times is associated with altered insulin sensitivity and higher body mass. Shift-work also leads to increased metabolic risks for cardio-metabolic syndrome, hypertension, inflammation.\n\nStudies conducted on both animals and humans show major bidirectional relationships between the circadian system and abusive drugs. It is indicated that these abusive drugs affect the central circadian pacemaker. Individuals suffering from substance abuse display disrupted rhythms. These disrupted rhythms can increase the risk for substance abuse and relapse. It is possible that genetic and/or environmental disturbances to the normal sleep and wake cycle can increase the susceptibility to addiction.\n\nWhat drove circadian rhythms to evolve has been an enigmatic question. Previous hypotheses emphasized that photosensitive proteins and circadian rhythms may have originated together in the earliest cells, with the purpose of protecting replicating DNA from high levels of damaging ultraviolet radiation during the daytime. As a result, replication was relegated to the dark. However, evidence for this is lacking, since the simplest organisms with a circadian rhythm, the cyanobacteria, do the opposite of this - they divide more in the daytime. Recent studies instead highlight the importance of co-evolution of redox proteins with circadian oscillators in all three kingdoms of life following the Great Oxidation Event approximately 2.3 billion years ago. The current view is that circadian changes in environmental oxygen levels and the production of reactive oxygen species (ROS) in the presence of daylight are likely to have driven a need to evolve circadian rhythms to preempt, and therefore counteract, damaging redox reactions on a daily basis.\n\nMutations or deletions of clock gene in mice have demonstrated the importance of body clocks to ensure the proper timing of cellular/metabolic events; clock-mutant mice are hyperphagic and obese, and have altered glucose metabolism. In mice, deletion of the Rev-ErbA alpha clock gene facilitates diet-induced obesity and changes the balance between glucose and lipid utilization predisposing to diabetes. However, it is not clear whether there is a strong association between clock gene polymorphisms in humans and the susceptibility to develop the metabolic syndrome.\n\nThe primary circadian \"clock\" in mammals is located in the suprachiasmatic nucleus (or nuclei) (SCN), a pair of distinct groups of cells located in the hypothalamus. Destruction of the SCN results in the complete absence of a regular sleep\u2013wake rhythm. The SCN receives information about illumination through the eyes. The retina of the eye contains \"classical\" photoreceptors (\"rods\" and \"cones\"), which are used for conventional vision. But the retina also contains specialized ganglion cells that are directly photosensitive, and project directly to the SCN, where they help in the entrainment (synchronization) of this master circadian clock.\n<Assistant>Thanks for the information. I've saved it.", "[Dialogue between User and Assistant on 2024-01-07 00:00]\n<User>Here are some news I learnt earlier:\nEarly research into circadian rhythms suggested that most people preferred a day closer to 25 hours when isolated from external stimuli like daylight and timekeeping. However, this research was faulty because it failed to shield the participants from artificial light. Although subjects were shielded from time cues (like clocks) and daylight, the researchers were not aware of the phase-delaying effects of indoor electric lights.[dubious \u2013 discuss] The subjects were allowed to turn on light when they were awake and to turn it off when they wanted to sleep. Electric light in the evening delayed their circadian phase.[citation needed] A more stringent study conducted in 1999 by Harvard University estimated the natural human rhythm to be closer to 24 hours, 11 minutes: much closer to the solar day but still not perfectly in sync.\n\nMore-or-less independent circadian rhythms are found in many organs and cells in the body outside the suprachiasmatic nuclei (SCN), the \"master clock\". These clocks, called peripheral oscillators, are found in the adrenal gland,[citation needed] oesophagus, lungs, liver, pancreas, spleen, thymus, and skin.[citation needed] Though oscillators in the skin respond to light, a systemic influence has not been proven. There is also some evidence that the olfactory bulb and prostate may experience oscillations when cultured, suggesting that these structures may also be weak oscillators.[citation needed]\n\nElizabeth's many historic visits and meetings include a state visit to the Republic of Ireland and reciprocal visits to and from the Pope. She has seen major constitutional changes, such as devolution in the United Kingdom, Canadian patriation, and the decolonisation of Africa. She has also reigned through various wars and conflicts involving many of her realms. She is the world's oldest reigning monarch as well as Britain's longest-lived. In 2015, she surpassed the reign of her great-great-grandmother, Queen Victoria, to become the longest-reigning British head of state and the longest-reigning queen regnant in world history.\n\nDuring the war, plans were drawn up to quell Welsh nationalism by affiliating Elizabeth more closely with Wales. Proposals, such as appointing her Constable of Caernarfon Castle or a patron of Urdd Gobaith Cymru (the Welsh League of Youth), were abandoned for various reasons, which included a fear of associating Elizabeth with conscientious objectors in the Urdd, at a time when Britain was at war. Welsh politicians suggested that she be made Princess of Wales on her 18th birthday. Home Secretary, Herbert Morrison supported the idea, but the King rejected it because he felt such a title belonged solely to the wife of a Prince of Wales and the Prince of Wales had always been the heir apparent. In 1946, she was inducted into the Welsh Gorsedd of Bards at the National Eisteddfod of Wales.\n\nElizabeth and Philip were married on 20 November 1947 at Westminster Abbey. They received 2500 wedding gifts from around the world. Because Britain had not yet completely recovered from the devastation of the war, Elizabeth required ration coupons to buy the material for her gown, which was designed by Norman Hartnell. In post-war Britain, it was not acceptable for the Duke of Edinburgh's German relations, including his three surviving sisters, to be invited to the wedding. The Duke of Windsor, formerly King Edward VIII, was not invited either.\n\nAmid preparations for the coronation, Princess Margaret informed her sister that she wished to marry Peter Townsend, a divorc\u00e9\u201a 16 years Margaret's senior, with two sons from his previous marriage. The Queen asked them to wait for a year; in the words of Martin Charteris, \"the Queen was naturally sympathetic towards the Princess, but I think she thought\u2014she hoped\u2014given time, the affair would peter out.\" Senior politicians were against the match and the Church of England did not permit remarriage after divorce. If Margaret had contracted a civil marriage, she would have been expected to renounce her right of succession. Eventually, she decided to abandon her plans with Townsend. In 1960, she married Antony Armstrong-Jones, who was created Earl of Snowdon the following year. They divorced in 1978; she did not remarry.\n\nThe Suez crisis and the choice of Eden's successor led in 1957 to the first major personal criticism of the Queen. In a magazine, which he owned and edited, Lord Altrincham accused her of being \"out of touch\". Altrincham was denounced by public figures and slapped by a member of the public appalled by his comments. Six years later, in 1963, Macmillan resigned and advised the Queen to appoint the Earl of Home as prime minister, advice that she followed. The Queen again came under criticism for appointing the prime minister on the advice of a small number of ministers or a single minister. In 1965, the Conservatives adopted a formal mechanism for electing a leader, thus relieving her of involvement.\n\nA year later, at the height of the 1975 Australian constitutional crisis, the Australian Prime Minister, Gough Whitlam, was dismissed from his post by Governor-General Sir John Kerr, after the Opposition-controlled Senate rejected Whitlam's budget proposals. As Whitlam had a majority in the House of Representatives, Speaker Gordon Scholes appealed to the Queen to reverse Kerr's decision. She declined, stating that she would not interfere in decisions reserved by the Constitution of Australia for the governor-general. The crisis fuelled Australian republicanism.\n<Assistant>Understood. I'll keep these facts for future reference.", "[Dialogue between User and Assistant on 2024-01-08 00:00]\n<User>Here's some more information I came across:\nIn 1987, in Canada, Elizabeth publicly supported politically divisive constitutional amendments, prompting criticism from opponents of the proposed changes, including Pierre Trudeau. The same year, the elected Fijian government was deposed in a military coup. Elizabeth, as monarch of Fiji, supported the attempts of the Governor-General, Ratu Sir Penaia Ganilau, to assert executive power and negotiate a settlement. Coup leader Sitiveni Rabuka deposed Ganilau and declared Fiji a republic. By the start of 1991, republican feeling in Britain had risen because of press estimates of the Queen's private wealth\u2014which were contradicted by the Palace\u2014and reports of affairs and strained marriages among her extended family. The involvement of the younger royals in the charity game show It's a Royal Knockout was ridiculed and the Queen was the target of satire.\n\nIn 2002, Elizabeth marked her Golden Jubilee. Her sister and mother died in February and March respectively, and the media speculated whether the Jubilee would be a success or a failure. She again undertook an extensive tour of her realms, which began in Jamaica in February, where she called the farewell banquet \"memorable\" after a power cut plunged the King's House, the official residence of the governor-general, into darkness. As in 1977, there were street parties and commemorative events, and monuments were named to honour the occasion. A million people attended each day of the three-day main Jubilee celebration in London, and the enthusiasm shown by the public for the Queen was greater than many journalists had expected.\n\nThe Queen, who opened the 1976 Summer Olympics in Montreal, also opened the 2012 Summer Olympics and Paralympics in London, making her the first head of state to open two Olympic Games in two different countries. For the London Olympics, she played herself in a short film as part of the opening ceremony, alongside Daniel Craig as James Bond. On 4 April 2013, she received an honorary BAFTA for her patronage of the film industry and was called \"the most memorable Bond girl yet\" at the award ceremony.\n\nElizabeth's personal fortune has been the subject of speculation for many years. Jock Colville, who was her former private secretary and a director of her bank, Coutts, estimated her wealth in 1971 at \u00a32 million (equivalent to about \u00a325 million today). In 1993, Buckingham Palace called estimates of \u00a3100 million \"grossly overstated\". She inherited an estimated \u00a370 million estate from her mother in 2002. The Sunday Times Rich List 2015 estimated her private wealth at \u00a3340 million, making her the 302nd richest person in the UK.\n\nTimes of personal significance have included the births and marriages of her children, grandchildren and great grandchildren, her coronation in 1953, and the celebration of milestones such as her Silver, Golden and Diamond Jubilees in 1977, 2002, and 2012, respectively. Moments of sadness for her include the death of her father, aged 56; the assassination of Prince Philip's uncle, Lord Mountbatten; the breakdown of her children's marriages in 1992 (her annus horribilis); the death in 1997 of her son's former wife, Diana, Princess of Wales; and the deaths of her mother and sister in 2002. Elizabeth has occasionally faced republican sentiments and severe press criticism of the royal family, but support for the monarchy and her personal popularity remain high.\n\nDespite the death of Queen Mary on 24 March, the coronation on 2 June 1953 went ahead as planned, as Mary had asked before she died. The ceremony in Westminster Abbey, with the exception of the anointing and communion, was televised for the first time.[d] Elizabeth's coronation gown was embroidered on her instructions with the floral emblems of Commonwealth countries: English Tudor rose; Scots thistle; Welsh leek; Irish shamrock; Australian wattle; Canadian maple leaf; New Zealand silver fern; South African protea; lotus flowers for India and Ceylon; and Pakistan's wheat, cotton, and jute.\n\nIn 1957, she made a state visit to the United States, where she addressed the United Nations General Assembly on behalf of the Commonwealth. On the same tour, she opened the 23rd Canadian Parliament, becoming the first monarch of Canada to open a parliamentary session. Two years later, solely in her capacity as Queen of Canada, she revisited the United States and toured Canada. In 1961, she toured Cyprus, India, Pakistan, Nepal, and Iran. On a visit to Ghana the same year, she dismissed fears for her safety, even though her host, President Kwame Nkrumah, who had replaced her as head of state, was a target for assassins. Harold Macmillan wrote, \"The Queen has been absolutely determined all through ... She is impatient of the attitude towards her to treat her as ... a film star ... She has indeed 'the heart and stomach of a man' ... She loves her duty and means to be a Queen.\" Before her tour through parts of Quebec in 1964, the press reported that extremists within the Quebec separatist movement were plotting Elizabeth's assassination. No attempt was made, but a riot did break out while she was in Montreal; the Queen's \"calmness and courage in the face of the violence\" was noted.\n\nIn 1977, Elizabeth marked the Silver Jubilee of her accession. Parties and events took place throughout the Commonwealth, many coinciding with her associated national and Commonwealth tours. The celebrations re-affirmed the Queen's popularity, despite virtually coincident negative press coverage of Princess Margaret's separation from her husband. In 1978, the Queen endured a state visit to the United Kingdom by Romania's communist dictator, Nicolae Ceau\u0219escu, and his wife, Elena, though privately she thought they had \"blood on their hands\". The following year brought two blows: one was the unmasking of Anthony Blunt, former Surveyor of the Queen's Pictures, as a communist spy; the other was the assassination of her relative and in-law Lord Mountbatten by the Provisional Irish Republican Army.\n<Assistant>Thanks for sharing. I've committed this to memory.", "[Dialogue between User and Assistant on 2024-01-09 00:00]\n<User>Here are some news I learnt earlier:\nIn the 1950s, as a young woman at the start of her reign, Elizabeth was depicted as a glamorous \"fairytale Queen\". After the trauma of the Second World War, it was a time of hope, a period of progress and achievement heralding a \"new Elizabethan age\". Lord Altrincham's accusation in 1957 that her speeches sounded like those of a \"priggish schoolgirl\" was an extremely rare criticism. In the late 1960s, attempts to portray a more modern image of the monarchy were made in the television documentary Royal Family and by televising Prince Charles's investiture as Prince of Wales. In public, she took to wearing mostly solid-colour overcoats and decorative hats, which allow her to be seen easily in a crowd.\n\nThe Royal Collection, which includes thousands of historic works of art and the Crown Jewels, is not owned by the Queen personally but is held in trust, as are her official residences, such as Buckingham Palace and Windsor Castle, and the Duchy of Lancaster, a property portfolio valued in 2014 at \u00a3442 million. Sandringham House and Balmoral Castle are privately owned by the Queen. The British Crown Estate \u2013 with holdings of \u00a39.4 billion in 2014 \u2013 is held in trust by the sovereign and cannot be sold or owned by Elizabeth in a private capacity.\n\nElizabeth's only sibling, Princess Margaret, was born in 1930. The two princesses were educated at home under the supervision of their mother and their governess, Marion Crawford, who was casually known as \"Crawfie\". Lessons concentrated on history, language, literature and music. Crawford published a biography of Elizabeth and Margaret's childhood years entitled The Little Princesses in 1950, much to the dismay of the royal family. The book describes Elizabeth's love of horses and dogs, her orderliness, and her attitude of responsibility. Others echoed such observations: Winston Churchill described Elizabeth when she was two as \"a character. She has an air of authority and reflectiveness astonishing in an infant.\" Her cousin Margaret Rhodes described her as \"a jolly little girl, but fundamentally sensible and well-behaved\".\n\nIn 1943, at the age of 16, Elizabeth undertook her first solo public appearance on a visit to the Grenadier Guards, of which she had been appointed colonel the previous year. As she approached her 18th birthday, parliament changed the law so that she could act as one of five Counsellors of State in the event of her father's incapacity or absence abroad, such as his visit to Italy in July 1944. In February 1945, she joined the Women's Auxiliary Territorial Service as an honorary second subaltern with the service number of 230873. She trained as a driver and mechanic and was promoted to honorary junior commander five months later.\n\nThe engagement was not without controversy: Philip had no financial standing, was foreign-born (though a British subject who had served in the Royal Navy throughout the Second World War), and had sisters who had married German noblemen with Nazi links. Marion Crawford wrote, \"Some of the King's advisors did not think him good enough for her. He was a prince without a home or kingdom. Some of the papers played long and loud tunes on the string of Philip's foreign origin.\" Elizabeth's mother was reported, in later biographies, to have opposed the union initially, even dubbing Philip \"The Hun\". In later life, however, she told biographer Tim Heald that Philip was \"an English gentleman\".\n\nDuring 1951, George VI's health declined and Elizabeth frequently stood in for him at public events. When she toured Canada and visited President Harry S. Truman in Washington, D.C., in October 1951, her private secretary, Martin Charteris, carried a draft accession declaration in case the King died while she was on tour. In early 1952, Elizabeth and Philip set out for a tour of Australia and New Zealand by way of Kenya. On 6 February 1952, they had just returned to their Kenyan home, Sagana Lodge, after a night spent at Treetops Hotel, when word arrived of the death of the King and consequently Elizabeth's immediate accession to the throne. Philip broke the news to the new Queen. Martin Charteris asked her to choose a regnal name; she chose to remain Elizabeth, \"of course\". She was proclaimed queen throughout her realms and the royal party hastily returned to the United Kingdom. She and the Duke of Edinburgh moved into Buckingham Palace.\n\nIn 1956, the British and French prime ministers, Sir Anthony Eden and Guy Mollet, discussed the possibility of France joining the Commonwealth. The proposal was never accepted and the following year France signed the Treaty of Rome, which established the European Economic Community, the precursor to the European Union. In November 1956, Britain and France invaded Egypt in an ultimately unsuccessful attempt to capture the Suez Canal. Lord Mountbatten claimed the Queen was opposed to the invasion, though Eden denied it. Eden resigned two months later.\n\nThe 1960s and 1970s saw an acceleration in the decolonisation of Africa and the Caribbean. Over 20 countries gained independence from Britain as part of a planned transition to self-government. In 1965, however, the Rhodesian Prime Minister, Ian Smith, in opposition to moves toward majority rule, declared unilateral independence from Britain while still expressing \"loyalty and devotion\" to Elizabeth. Although the Queen dismissed him in a formal declaration, and the international community applied sanctions against Rhodesia, his regime survived for over a decade. As Britain's ties to its former empire weakened, the British government sought entry to the European Community, a goal it achieved in 1973.\n\nDuring the 1981 Trooping the Colour ceremony and only six weeks before the wedding of Charles, Prince of Wales, and Lady Diana Spencer, six shots were fired at the Queen from close range as she rode down The Mall on her horse, Burmese. Police later discovered that the shots were blanks. The 17-year-old assailant, Marcus Sarjeant, was sentenced to five years in prison and released after three. The Queen's composure and skill in controlling her mount were widely praised. From April to September 1982, the Queen remained anxious but proud of her son, Prince Andrew, who was serving with British forces during the Falklands War. On 9 July, the Queen awoke in her bedroom at Buckingham Palace to find an intruder, Michael Fagan, in the room with her. Remaining calm and through two calls to the Palace police switchboard, she spoke to Fagan while he sat at the foot of her bed until assistance arrived seven minutes later. Though she hosted US President Ronald Reagan at Windsor Castle in 1982 and visited his Californian ranch in 1983, she was angered when his administration ordered the invasion of Grenada, one of her Caribbean realms, without informing her.\n\nIn the years to follow, public revelations on the state of Charles and Diana's marriage continued. Even though support for republicanism in Britain seemed higher than at any time in living memory, republicanism was still a minority viewpoint, and the Queen herself had high approval ratings. Criticism was focused on the institution of the monarchy itself and the Queen's wider family rather than her own behaviour and actions. In consultation with her husband and the Prime Minister, John Major, as well as the Archbishop of Canterbury, George Carey, and her private secretary, Robert Fellowes, she wrote to Charles and Diana at the end of December 1995, saying that a divorce was desirable.\n\nThe Queen addressed the United Nations for a second time in 2010, again in her capacity as Queen of all Commonwealth realms and Head of the Commonwealth. The UN Secretary General, Ban Ki-moon, introduced her as \"an anchor for our age\". During her visit to New York, which followed a tour of Canada, she officially opened a memorial garden for the British victims of the September 11 attacks. The Queen's visit to Australia in October 2011 \u2013 her sixteenth visit since 1954 \u2013 was called her \"farewell tour\" in the press because of her age.\n\nFrom 21 April 1944 until her accession, Elizabeth's arms consisted of a lozenge bearing the royal coat of arms of the United Kingdom differenced with a label of three points argent, the centre point bearing a Tudor rose and the first and third a cross of St George. Upon her accession, she inherited the various arms her father held as sovereign. The Queen also possesses royal standards and personal flags for use in the United Kingdom, Canada, Australia, New Zealand, Jamaica, Barbados, and elsewhere.\n\nElizabeth was born in London to the Duke and Duchess of York, later King George VI and Queen Elizabeth, and was the elder of their two daughters. She was educated privately at home. Her father acceded to the throne on the abdication of his brother Edward VIII in 1936, from which time she was the heir presumptive. She began to undertake public duties during World War II, serving in the Auxiliary Territorial Service. In 1947, she married Philip, Duke of Edinburgh, with whom she has four children: Charles, Anne, Andrew, and Edward.\n<Assistant>Noted. I'll remember these details.", "[Dialogue between User and Assistant on 2024-01-10 00:00]\n<User>I have some fresh insights to share:\nDuring her grandfather's reign, Elizabeth was third in the line of succession to the throne, behind her uncle Edward, Prince of Wales, and her father, the Duke of York. Although her birth generated public interest, she was not expected to become queen, as the Prince of Wales was still young, and many assumed that he would marry and have children of his own. When her grandfather died in 1936 and her uncle succeeded as Edward VIII, she became second-in-line to the throne, after her father. Later that year Edward abdicated, after his proposed marriage to divorced socialite Wallis Simpson provoked a constitutional crisis. Consequently, Elizabeth's father became king, and she became heir presumptive. If her parents had had a later son, she would have lost her position as first-in-line, as her brother would have been heir apparent and above her in the line of succession.\n<Assistant>Appreciated. I'll retain these important details."]
[{"question": "What former King was not invited to the wedding?", "answer": "Duke of Windsor"}, {"question": "What was the fault not considered in the early theories of day length?", "answer": "artificial light"}, {"question": "What do studies show has a bidirectional relationship with the circadian system?", "answer": "abusive drugs"}, {"question": "At what age was Elizabeth when she went on her first solo public appearance?", "answer": "16"}, {"question": "What aninmals  do researchers think might also show a variance in circadian rhythms in different seasons?", "answer": "other Arctic animals"}, {"question": "By better using resources, how does that improve an organism's chances of surviving?", "answer": "selective advantage"}, {"question": "What time cycle did studies in 1938 and 1990s use on humans?", "answer": "28-hour"}, {"question": "Before the Enlightenment, most intellectual debates revoled around what subject?", "answer": "\"confessional\""}, {"question": "What residences of Elizabeth are held in trust and not owned by Elizabeth?", "answer": "official residences"}, {"question": "During what ceremony were shots fired at the Queen?", "answer": "Trooping the Colour"}, {"question": "What type of flags are used in foreign countries?", "answer": "personal"}, {"question": "At its onset, what can be measured in blood or saliva?", "answer": "Melatonin"}, {"question": "When did Elizabeth support constitutional amendments in Canada?", "answer": "1987"}, {"question": "What do blind mole rats have in place of vision?", "answer": "photoreceptors"}, {"question": "What parliamentary session did Elizabeth open in 1957 while on tour?", "answer": "23rd Canadian Parliament"}, {"question": "What was the term given to books that did not have a dominant motif and contained something to offend almost everyone in authority?", "answer": "general works"}, {"question": "How many children did Elizabeth's parents have?", "answer": "two daughters"}, {"question": "Where are these cell groups found in humans?", "answer": "hypothalamus"}, {"question": "How long before wake time is the lowest temperature reached?", "answer": "two hours"}, {"question": "What island's invasion angered Elizabeth?", "answer": "Grenada"}, {"question": "When was Elizabeth's farewell visit to Australia?", "answer": "October 2011"}, {"question": "What type of feedback mechanism was previously thought to be needed to sustain the clock?", "answer": "DNA"}, {"question": "What do Jonathon Israel claim caused the change that eventually led to the revolutions of the latter half of the 18th centure and early 19th century?", "answer": "the ideas themselves"}, {"question": "Which work narrowly escaped seizure and had to be saved by Malesherbes?", "answer": "The Encyclop\u00e9die"}, {"question": "How is the British Crown Estate held by Elizabeth?", "answer": "in trust"}, {"question": "What do drug abusers show in their circadian processes?", "answer": "disrupted rhythms"}, {"question": "What effect does jet-lag and shift-work have on the human body?", "answer": "profound consequences"}, {"question": "What is theorized to have evolved with circadian rhythms?", "answer": "photosensitive proteins"}, {"question": "What did Edward's proposed marriage to Simpson cause?", "answer": "constitutional crisis"}, {"question": "What provides information to the SCN?", "answer": "eyes"}, {"question": "Historian Margaret Jacob stresses the importance of the Masons in indirectly inspriting what type of thinking?", "answer": "political thought"}, {"question": "Who was the always the Prince of Wales?", "answer": "heir apparent"}, {"question": "What do body hormone receptors do with the body's organs?", "answer": "synchronise"}, {"question": "What was considered the trunk of the tree of knowledge?", "answer": "philosophy"}, {"question": "When did more stringent testing determine that humans preferred a 24 hour day?", "answer": "1999"}, {"question": "By improving what processes does the use of circadian rhythms serve to benefit the individual?", "answer": "internal metabolic"}, {"question": "What do abusive drugs effect in the circadian system?", "answer": "circadian pacemaker"}, {"question": "What conditions will produce a free-running rhythm in humans?", "answer": "total darkness"}, {"question": "Besides personal flags,what other types of flags does Elizabeth have?", "answer": "royal standards"}, {"question": "When did Elizabeth join the Women's Auxiliary Territorial Service?", "answer": "February 1945"}, {"question": "Which society in England also played a significant role in the public sphere and spread of Enlightenment ideas?", "answer": "Royal Society of London"}, {"question": "What actor also appeared with Elizabeth in the film?", "answer": "Daniel Craig"}, {"question": "What did Immanuel Kant consider essential to his conception of the public sphere?", "answer": "written communication"}, {"question": "What are the more reliable markers in determining sleep timing?", "answer": "melatonin phase"}, {"question": "For what activity related to the film industry did Elizabeth receive a BAFTA award?", "answer": "patronage"}, {"question": "What ship captain described daily leaf movements?", "answer": "Androsthenes"}, {"question": "What produces a self-sustaining rhythm?", "answer": "central oscillator"}, {"question": "Who was Elizabeth only sibling?P", "answer": "Princess Margaret"}, {"question": "Who, in 1979, was discovered to be a communist spy?", "answer": "Anthony Blunt"}, {"question": "Besides insulin sensitivity, what other effect does shift-work have on the body?", "answer": "higher body mass"}, {"question": "What insect has been studied concerning the inheritability of rhythms?", "answer": "fruit flies"}, {"question": "Who did Elizabeth appoint as Prime Minister after the resignation of Macmillan?", "answer": "Earl of Home"}, {"question": "When in the cycle do wake-sleep cycles break off from the circadian period?", "answer": "24.18 hours"}, {"question": "Many publishing companies were located outside of which country to avoid overzealous censors?", "answer": "France"}, {"question": "What section of the brain periodically releases hormones?", "answer": "endocrine glands"}, {"question": "Who wrote the celebrated Philosophiae Naturalis Principia Mathematica?", "answer": "Sir Isaac Newton"}, {"question": "Who were considered more reliable witnesses than the Oxfordshire peasants?", "answer": "Oxford professors"}, {"question": "Who appealed to Elizabeth to reverse the dismissal of Whitlam?", "answer": "Speaker Gordon Scholes"}, {"question": "To what do oscillators in the skin respond?", "answer": "light"}, {"question": "When did Princess Margaret separate from her husband?", "answer": "1977"}, {"question": "How many dictionaries and encyclopedias were published between 1760 and 1769?", "answer": "63"}, {"question": "When did the Chinese use diurnal rhythms to remember Acu-points?", "answer": "13th century"}, {"question": "What Commonwealth symbols were embroidered on Elizabeth's gown?", "answer": "floral emblems"}, {"question": "In 1991 what money issue of the Queen was a feature of public criticism?", "answer": "private wealth"}, {"question": "What is the plant expression of circadian rhythm?", "answer": "when to flower"}, {"question": "Which Scottish professor believes that Masons were not a major radical or reformist network in their own right?", "answer": "Thomas Munck"}, {"question": "On a tour to Ghana in 1961 what did Elizabeth dismiss as a fear?", "answer": "her safety"}, {"question": "What was the title of the book written by Crawford about the princesses?", "answer": "The Little Princesses"}, {"question": "Whose method based knowledge on experiementation, which had to be witnessed?", "answer": "Robert Boyle"}, {"question": "Catholic, Lutheran, Reformed (Calvinist), or Anglican issues were called what?", "answer": "\"confessional\""}, {"question": "Where did Elizabeth visit on her first solo public appearance?", "answer": "Grenadier Guards"}, {"question": "To what did the journals shift attention of the cultivated public away from and towards novelty and innovation?", "answer": "established authorities"}, {"question": "What researcher showed that the gene  feedback loop serves as a repressor?", "answer": "Andrew Millar"}, {"question": "What does the body interpret from the gene feedback loop?", "answer": "time of the day"}, {"question": "Inside of what can the molecular circadian clock operate?", "answer": "single cell"}, {"question": "Which philosopher believed that the Enlightenment was ultimately born out of Protestant reaction against the Catholic counter-reformation?", "answer": "Bertrand Russell"}, {"question": "Who did England propose to affiliate with Wales to quell Welsh nationalism?", "answer": "Elizabeth"}, {"question": "What is the term for the independent clocks?", "answer": "peripheral oscillators"}, {"question": "What political feeling is still a minority view in Britain?", "answer": "republicanism"}, {"question": "Jonathon Israel rejects the notion that the revolutionary ideas were by products of what transformations?", "answer": "social and economic"}, {"question": "What group was surprised by the public's approval of the Queen?", "answer": "journalists"}, {"question": "What did Queen Elizabeth ask Margaret to do instead of marring Townsend?", "answer": "wait for a year"}, {"question": "What aspect of the Enlightenment did Robert Darnton and Jurgen Habermas focus on within the Enlightenment?", "answer": "social conditions"}, {"question": "What people have a thistle for their Commonwealth emblem?", "answer": "Scots"}, {"question": "One possible reason for the development of the circadian system is the need to counteract what ?", "answer": "redox reactions"}, {"question": "How long did the suppression of time clues study last?", "answer": "month"}, {"question": "What term did Habermas give to describe the areas of political/social knowledge and discussion that were previously exlusive territory of the state and religious authorities?", "answer": "common concern"}, {"question": "Animals that eat during resting periods show what body increase?", "answer": "body mass"}, {"question": "How certain is it that these circadian clock  effects are the same in humans?", "answer": "not clear"}, {"question": "From what did  DNA need to be protected in the earliest cells?", "answer": "ultraviolet radiation"}, {"question": "Aside from her choice of Eden's successor, what crisis caused Elizabeth to be criticized?", "answer": "Suez crisis"}, {"question": "How are sleep and wake cycles as well as body functions coordinated?", "answer": "biological clock"}, {"question": "How many dictionaries and encyclopedias were published in the decade preceding the French Revolution?", "answer": "148"}, {"question": "Who believed the avoidance of thematic and heirarhical systems allowed free interpretation of the works and caused them to beomce an example of eglitarianism?", "answer": "Charles Porset"}, {"question": "What would the loss of the SCN cells cause in the sleep-wake rhythm?", "answer": "complete absence"}, {"question": "When does the temperature of morning type young adults reach its lowest?", "answer": "04:00"}, {"question": "Which ideal endeavoured to make information available to the greatest number of people?", "answer": "Popularization"}, {"question": "Whose work habits and environment prevents them from maintaining a regular sleep pattern?", "answer": "airline pilots"}, {"question": "At what degree north did reindeer show rhythms only in autumn and summer?", "answer": "78 degrees"}, {"question": "In what capacity did Elizabeth serve in the Auxiliary?", "answer": "driver and mechanic"}]