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fruits of citrus aurantifolia ( christm ) were collected from gardens near the shiraz city , fars province , iran in summer of 2008 , and identified by a botanist at the biology department , science faculty of isfahan university , isfahan , iran . a voucher specimen ( 5527 )
the whole peal of fruits was dried at room , pulverized and polyphenolic extract was prepared .
ldl was separated from one human serum sample by a double step density gradient ultracentrifugation by modificated bronzert and brewer method.9 at first step ( vldl separation ) a discontinued gradient was formed from bottom to top with 6 ml of serum and 3 ml of 1.006 g / cm3 solution a. the centrifugation was carried out in a beckman coulter 90ti rotor in a beckman optimal x-100 ultracentrifuge at 60000 rpm10h at 16c .
immediately after centrifugation , the ldl fraction was collected , and dialyzed for 24 h at 4c in dark place against 0.01
the buffer was changed three times during the dialysis period and purified ldl was stored at -80c .
protein concentration of the ldl sample was determined by lowry method.10 the separated ldl sample was used for studying antioxidant effects of both juice and peel extract , and oxidation process was done in completely similar status .
10 l ldl ( protein concentration of 150 g / ml ) was diluted in 827 l pbs buffer .
then , after adding 250 l cuso4 solution ( 5 m ) , oxidation of ldl was started .
oxidation was followed by measuring the absorption of conjugated dienes at 234 nm at 10-min intervals for nearly 500 minutes at 37c in a shimadzu uv / vis spectrophotometer .
ldl oxidation was measured against 0 , 5 , 10 , 20 , 25 , 30 and 40 l of fresh lime juice and 0 , 5 , 10 , 15 and 20 l of peel poly phenolic extract in dmso solution ( stock solution : 3.4 mg / ml ) . the juice and extract used
the ldl oxidation did nt change using 5 l fresh lime juice ( figure 1 ) .
but , 10 l of juice inhibited ldl oxidation , and after increasing lime juice concentration , ldl was oxidized faster and lag time of oxidation curve was shorter . according to figure 2 ,
the higher concentration of peel extract prevented ldl oxidation better than lower ones and lag time of oxidation curve became higher .
effect of different concentrations of fresh lime juice on ldl oxidation effect of different concentrations of lime peel extract on ldl oxidation
in this study it is found that the peel and fresh juice of lime have different effects on ldl oxidation .
the low concentration of lime juice ( 5l ) did not show considerable effect on ldl oxidation , in vitro .
but , antioxidant activity of lime juice was observed only after applying increased concentration of 10 l with ldl oxidative inhibition . beyond this concentration
therefore , it was demonstrated that peak stimulation for lime juice is 10 l for 150 g / ml ldl . additionally , oxidation curve is a proof for a dose - independent response of antioxidant effect found for the lime juice .
it reflects that flavonoids composition and other component of lime juice do not show similar effects .
regarding the extract , the peak stimulation was 20 l ( 158 mg / ml of stock extract ) with antioxidant activity to inhibit ldl oxidation . comparing the two figures
, it seems that flavonoids contents of the extract show their expected and natural effects but some or all flavonoids in lime juice reveal inverted - u response . regarding the intensity and type , lime juice and peel appear to include flavonoids with different effects in this study .
for instance , naringin , one of the flavonoids in lime juice , showed the chemo - preventive effect in an experiment and has proved mild anti - oxidative effects on lipid peroxidation in another study.2 on the other hand , although in some reports , an antioxidant effect has been demonstrated for this flavonoid , flavonoids containing phenol b rings , such as naringin , hesperidin , and apigenin have been implicated in the initiation of atherosclerosis and carcinogenesis.12 moreover , these flavonoids act as independent pro - oxidants in autoxidation reactions catalyzed by transition metal.2 also , it should be noted that aglycones of some flavonoids like naringin and hesperidin have less antiperoxidative potential compared to their corresponding aglycones.13 in addition , a study has reported a weak antiperoxidative capacity for naringin when no decrease was observed in plasma and hepatic tbars levels after its supplementation with a high - cholesterol diet.2 as mentioned before , flavonoids showed different antioxidant effects .
for example , a research in japan showed that tea flavonoids reduced oxidizability of ldl in vitro and in vivo.14 vitamin c is another effective component in lime juice.15 it is a predominant reducing agent known to act as an antioxidant in vitro and in vivo.16 vitamin c protects human plasma lipids and ldl against peroxidative damage induced by various types of oxidants.3 compared to other endogenous antioxidants in plasma like vitamin e which is soluble in human lipids , this vitamin inhibits lipid peroxidation.1720 so , the inverted - u dose response for lime juice maybe related to the simultaneous effect of vitamin c together with some or all flavonoids in lime juice or inversely , they may dilute an intensive inverted - u dose response in one of its components .
perhaps , this response is attributable to some unknown effective components in limes cultivated in iran , different from ones cited so far , or this property might belong to 5000 trace plant flavonoids have not been determined till now .
this is aligned with the 50 percent - decreased effects for the industrially processed juices compared to the hand - squeezed ones other studies reported.15 the results obtained from the extracts are logic .
it seems that all flavonoids in the extract have antioxidant role and increasing their concentrations causes antioxidant role to be elevated .
we concluded that in contrast to lime peel , the excessive consumption of lime juice is not only beneficial but also harmful due to an inverted - u dose response and only intermediate effective concentration showed antioxidant effect .
therefore , the recommendation for consumption of this fruit especially its peel seems useful for all people but in vivo human studies are needed for any dietary recommendation .
mb was the coordinator of the study , analyzed the data and prepared the manuscript .
jm and ghn have assisted in designing the study , coordinated all the experiments and participated in preparing the manuscript .
sa and hn provided assistance in the study design and participated in preparing the manuscript .
background : we studied the antioxidant effects of fresh juice and peel extract of citrus aurantifolia ( christm).methods : low density lipoprotein ( ldl ) was separated from one hypercholesterolemic human serum by modified bronzert and brewer procedure . 
 oxidation of ldl was measured at 234 nm against 0 , 5 , 10 , 20 , 25 , 30 and 40 l of fresh lime juice and 0 , 5 , 10 , 15 and 20 l of peel polyphenolic extract solution in dmso.results:5 l of lime juice did nt change ldl oxidation . 
 10 l of juice inhibited ldl oxidation , and with increasing the juice concentration , ldl was oxidized faster . 
 the higher concentrations of peel extract prevented ldl oxidation better than the lower ones.conclusions:both juice and peel demonstrated antioxidant properties , but the excessive consumption of lime juice seems not to be beneficial . regarding the intensity and type of flavonoids , lime juice and peel may show different effects .