You will be shown a claim related to the COVID-19 pandemic, and the abstract of a biomedical research paper. Each sentence from the abstract will be on a separate line. Your task is to return a JSON object with two fields:

- "verdict": The fact-checking verdict. If the information in the abstract supports the claim, write "SUPPORT". If the abstract contradicts the claim, write "CONTRADICT".
- "evidence": An array of sentences providing evidence for the verdict. Please copy all relevant sentences verbatim from the abstract.

For instance, if the model were given the claim "the COVID vaccine is safe for healthy adults", the output might be
{
  "verdict": "SUPPORT",
  "evidence": ["Our findings indicate that the risks of severe COVID vaccine side effects are low for healthy adults."]
}

Claim: Insight into vaccine development for alpha-coronaviruses based on structural and immunological analyses of spike genes

Abstract:
Coronaviruses that infect humans belong to the Alpha-coronavirus (including HCoV-229E) and Beta-coronavirus (including SARS-CoV and SARS-CoV-2) genera.
In particular, SARS-CoV-2 is currently a major threat to public health worldwide.
However, no commercial vaccines against the coronaviruses that can infect humans are available.
The spike (S) homotrimers bind to their receptors through the receptor-binding domain (RBD), which is believed to be a major target to block viral entry.
In this study, we selected Alpha-coronavirus (HCoV-229E) and Beta-coronavirus (SARS-CoV and SARS-CoV-2) as models.
Their RBDs were observed to adopt two different conformational states (lying or standing).
Then, structural and immunological analyses were used to explore differences in the immune response with RBDs among these coronaviruses.
Our results showed that more RBD-specific antibodies were induced by the S trimer with the RBD in the “standing” state (SARS-CoV and SARS-CoV-2) than the S trimer with the RBD in the “lying” state (HCoV-229E), and the affinity between the RBD-specific antibodies and S trimer was also higher in the SARS-CoV and SARS-CoV-2.
In addition, we found that the ability of the HCoV-229E RBD to induce neutralizing antibodies was much lower and the intact and stable S1 subunit was essential for producing efficient neutralizing antibodies against HCoV-229E.
Importantly, our results reveal different vaccine strategies for coronaviruses, and S-trimer is better than RBD as a target for vaccine development in Alpha-coronavirus.
Our findings will provide important implications for future development of coronavirus vaccines.
Importance Outbreak of coronaviruses, especially SARS-CoV-2, poses a serious threat to global public health.
Development of vaccines to prevent the coronaviruses that can infect humans has always been a top priority.
Coronavirus spike (S) protein is considered as a major target for vaccine development.
Currently, structural studies have shown that Alpha-coronavirus (HCoV-229E) and Beta-coronavirus (SARS-CoV and SARS-CoV-2) RBDs are in lying and standing state, respectively.
Here, we tested the ability of S-trimer and RBD to induce neutralizing antibodies among these coronaviruses.
Our results showed that Beta-CoVs RBDs are in a standing state, and their S proteins can induce more neutralizing antibodies targeting RBD.
However, HCoV-229E RBD is in a lying state, and its S protein induces a low level of neutralizing antibody targeting RBD.
Our results indicate that Alpha-coronavirus is more conducive to escape host immune recognition, and also provide novel ideas for the development of vaccines targeting S protein.
{"verdict": "CONTRADICT", "evidence": ["Then, structural and immunological analyses were used to explore differences in the immune response with RBDs among these coronaviruses.", "Coronavirus spike (S) protein is considered as a major target for vaccine development.", "Our results indicate that Alpha-coronavirus is more conducive to escape host immune recognition, and also provide novel ideas for the development of vaccines targeting S protein."]}