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e. cuniculi is a cause of serious disease - encephalitozoonosis in animals and people ( 48 ) . in recent years ,
subclinical e. cuniculi infection usually develops in immunocompetent hosts ( 9 ) . in the world , the infections have been reported in chile , canada , france , germany , japan , italy , mexico , spain , the united kingdom , the united states , and in switzerland in immunocompromised ( without / with hiv infection ) humans ( 2 , 4 , 5 , 10 ) .
the detection of the parasite has increased considerably since the existence of infection in immunocopromised and immunocompetent human ( 4 ) . in turkey , only four studies have been reported to date of microsporidiosis in human , while animal encephalitozonosis has first been described histopathologically ( 11 ) and later in rabbits using the carbon immuno - assay ( cia ) test ( 12 , 13 ) and histopathologically as the diagnostic method ( 14 ) . in our previous study , it was shown that rabbits ( fig .
1 ) were infected with e. cuniculi in a breeding colony and an animal care worker was also found to be seropositive in turkey ( 13 ) .
the present study was to evaluate the health status examination of this seropositive animal care worker .
blood samples were taken into evacuated tubes from the vena cubiti of the five workers .
the cia test kit was purchased from medicago ( medicago , uppsala , sweden ) .
, the e. cuniculi spores were stained greyish - black against the background of the carbon particles , and with negative sera , the parasite appeared white on the dark background . in assay ,
negative and positive control sera ( supplied by medicago ) were run in each test .
following air - drying , the wells were covered with 15 l of rabbit anti - human igg marked fluorescein isothiocyanate conjugate ( fitc , sigma ) of 1:160 dilution .
after 30 min , the slides were washed and then were mounted using fluoromount - g .
both a positive and negative rabbit serum ( supplied by medicago ) were included in this test as control . in the case of a positive immunological reaction , the spores with strong peripheral fluorescence were observed as oval formations of 1.52.5 m in size and were considered positive . in paralel of cia test ,
specimen of urinary sediment ( 10 l ) was stained with modified trichrome stain ( ryan - blue ) for the detection of e. cuniculi spores as described by ryan et al .
diluted antigen from the cia kit was used as a positive control for the accuracy of the stain method .
seropositive worker had a complete physical examination , medical history , serum biochemical parameters and hematological parameters , chest and sinus x - ray and total abdominal ultrasonography ( usg ) at the ozkaya medical center , ankara .
blood samples were taken into evacuated tubes from the vena cubiti of the five workers .
the cia test kit was purchased from medicago ( medicago , uppsala , sweden ) .
, the e. cuniculi spores were stained greyish - black against the background of the carbon particles , and with negative sera , the parasite appeared white on the dark background . in assay ,
negative and positive control sera ( supplied by medicago ) were run in each test .
, the wells were covered with 15 l of rabbit anti - human igg marked fluorescein isothiocyanate conjugate ( fitc , sigma ) of 1:160 dilution .
after 30 min , the slides were washed and then were mounted using fluoromount - g .
both a positive and negative rabbit serum ( supplied by medicago ) were included in this test as control . in the case of a positive immunological reaction , the spores with strong peripheral fluorescence were observed as oval formations of 1.52.5 m in size and were considered positive . in paralel of cia test , a 1:40 cut - off was used to identify a sample as positive .
specimen of urinary sediment ( 10 l ) was stained with modified trichrome stain ( ryan - blue ) for the detection of e. cuniculi spores as described by ryan et al .
. diluted antigen from the cia kit was used as a positive control for the accuracy of the stain method .
seropositive worker had a complete physical examination , medical history , serum biochemical parameters and hematological parameters , chest and sinus x - ray and total abdominal ultrasonography ( usg ) at the ozkaya medical center , ankara .
the cia test kit was purchased from medicago ( medicago , uppsala , sweden ) .
, the e. cuniculi spores were stained greyish - black against the background of the carbon particles , and with negative sera , the parasite appeared white on the dark background . in assay ,
negative and positive control sera ( supplied by medicago ) were run in each test .
, the wells were covered with 15 l of rabbit anti - human igg marked fluorescein isothiocyanate conjugate ( fitc , sigma ) of 1:160 dilution .
after 30 min , the slides were washed and then were mounted using fluoromount - g .
both a positive and negative rabbit serum ( supplied by medicago ) were included in this test as control . in the case of a positive immunological reaction , the spores with strong peripheral fluorescence were observed as oval formations of 1.52.5 m in size and were considered positive . in paralel of cia test ,
specimen of urinary sediment ( 10 l ) was stained with modified trichrome stain ( ryan - blue ) for the detection of e. cuniculi spores as described by ryan et al .
diluted antigen from the cia kit was used as a positive control for the accuracy of the stain method .
seropositive worker had a complete physical examination , medical history , serum biochemical parameters and hematological parameters , chest and sinus x - ray and total abdominal ultrasonography ( usg ) at the ozkaya medical center , ankara .
only one worker was found to be seropositive according to the results of the serological test .
2a ) and spores were also detected in the urine sample of the seropositive worker ( fig .
2b ) after staining with modified trichrome stain . a : 
 encephalitozoon cuniculi spores stained apple - green by indirect immunofluorescence with anti 
b : spores of e. cuniculi from urine stained with trichrome blue ( magnification 1000 ) .
laboratory tests were performed to evaluate different biochemical parameters and hemato - logical parameters ( table 1 ) .
laboratory examination shown in table 1 , the complement 3 ( c3 ) , c4 , iga , igm and igg were measured within reference interval .
the c - reactive protein concentration was 6 mg / l ( 010 mg / l ) , and the creatinine concentration was 0.95 mg / dl ( 0.51.4 mg / dl ) .
the chest film , the frontal and maxillary sinuses and total abdominal usg did not reveal any lesions .
laboratory finding to evaluate different biochemical parameters and hematological parameters his visual acuity and visual field were normal , as were the results of funduscopy and there was no keratoconjunctivitis .
the urine samples at two weeks interval ( 3 times : the beginning , the third week beginning and the fifth week beginning ) were collected to detect the spores using trichrome staining after albendazole treatment .
only one worker was found to be seropositive according to the results of the serological test .
2a ) and spores were also detected in the urine sample of the seropositive worker ( fig .
2b ) after staining with modified trichrome stain . a : 
 encephalitozoon cuniculi spores stained apple - green by indirect immunofluorescence with anti 
b : spores of e. cuniculi from urine stained with trichrome blue ( magnification 1000 ) .
laboratory tests were performed to evaluate different biochemical parameters and hemato - logical parameters ( table 1 ) . laboratory examination shown in table 1 , the complement 3 ( c3 ) , c4 , iga , igm and igg were measured within reference interval .
the c - reactive protein concentration was 6 mg / l ( 010 mg / l ) , and the creatinine concentration was 0.95 mg / dl ( 0.51.4 mg / dl ) .
the chest film , the frontal and maxillary sinuses and total abdominal usg did not reveal any lesions .
laboratory finding to evaluate different biochemical parameters and hematological parameters his visual acuity and visual field were normal , as were the results of funduscopy and there was no keratoconjunctivitis .
the urine samples at two weeks interval ( 3 times : the beginning , the third week beginning and the fifth week beginning ) were collected to detect the spores using trichrome staining after albendazole treatment .
buget et al . ( 17 ) reported the first microsporidiosis in turkey and it was detected in stool samples of an aids patient .
later , yazar et al . ( 18 ) reported spores of microsporidia in the brochoalveolar lavage sample of a patient with acute myeloblastic leukaemia - m3 .
in addition , atambay et al . ( 19 ) investigated in no immunesuppressive problem patients for intestinal parasites and microsporidium .
( 20 ) found microsporidium 10.9 % in the patients with cancer while 5.6 % in non - cancer patient .
species of microsporidia have not been identified in all reports ; however microsporidiosis is reported in immunocopromised as well as in immunocompetent individuals . in turkey
to our knowledge , this is the first encephalitozoon infection reported in an immunocompetent person .
first encephalitozoon infection was reported in a boy and has been identified as a human pathogen in 1959 ( 2 , 3 , 21 ) . in rabbits ,
e. cuniculi spores are finally localized in kidneys , eyes and brain causing clinical signs such as cataracts , head tilt , paralysis and death ( 22 ) .
adult rabbits are infected by ingestion or inhalation of e. cuniculi spores , which are passed in the urine ( 23 ) but are excreted only sporadically ( 24 ) .
nevertheless , microsporidian spores are mostly isolated from the urine samples depend on immune status of patients according to previously case reports or studies . in our study , 24h urine sample was repeatedly collected from an animal care worker for detection of spores but only one sample was detected in urine . in a recent serodiagnostic study ,
an immunocompetent laboratory worker was accidentally infected with e. cuniculi ( 2 ) . in our study ,
general health status of the seropositive worker was investigated even though it had not been in any medical complaint .
therefore e. cuniculi is a widespread microsporidian species infecting primates , rodents , lagomorphs and carnivores , and has also been found in immunocompetent humans ( 5 ) .
direct transmission between animals and human has not been reported and the portal of entry of this pathogen remains unknown ( 7 , 25 ) .
however , rabbits and dogs may play an important role as potential sources of e. cuniculi infection for humans according to histories of previously reported cases .
e. cuniculi spores are also highly resistant in the environment and can survive several months under humid conditions .
therefore , oral transmission by contaminated drinking water or food and airborne transmission and it may be possible that all play a part in the infective process ( 5 , 7 , 26 ) . in our study , the person had worked with rabbits in the breeding department for a long time . as a consequence ,
the disease may have been transmitted from infected rabbits to the worker because the results of the questionnaire also showed that he had ignored personal hygiene while washing cages and handling the animals during working time .
albendazole has been successfully used in patients with e. cuniculi infection ( 27 ) . in our study , therefore the worker was given albendazole at 200 mg twice daily for 2 weeks .
the urine sample of the worker was examined to identify the spores at three times in the different periods after treatment .
( a ) rabbits should be examined routinely for the presence of anti - e .
cuniculi antibody even if apparently healthy , especially considering its potential zoonotic risk ; ( b ) people working with animal should avoid contact with urine and faeces of infected or healthy animals even though e. cuniculi spores were not detected in the worker urine sample and ( c ) always use good personal hygiene when handling animals .
background : as a zoonotic pathogen , encephalitozoon cuniculi is a cause of serious disease in animals and people . 
 the present study was to evaluate the health status examination of this seropositive animal care worker in our previous study.methods:blood samples were taken from five workers . 
 cia test was applied to detect antibodies against e. cuniculi in blood serum . 
 the indirect immunofluorescence antibody test was used as confirmation test . 
 seropositive worker had a complete medical examination.results:only one worker was found to be seropositive according to the results of the serological test . 
 sera positive to e. cuniculi was confirmed with ifat and spores were detected in the urine sample of the worker . 
 the worker was treated with albendazole.conclusion:rabbits should be examined routinely for the presence of anti - e . 
 cuniculi antibody . 
 people working with laboratory animal should avoid contact with urine and faeces of infected or pay attention to personal hygiene .