You will be presented with the full text of a biomedical research paper. Please write an abstract for this paper. Your response should include the abstract and no additional text.

Paper text:
you grew up in new york state and stayed for college , graduate school , and your postdoc .
i had a pretty strong case of insecurity and the imposter syndrome until i was about 45 .
i liken it to the scene in a beautiful mind , in which the economist john nash realizes that the little girl in his life is a delusion because over several years she has not grown up .
one day flying out to california to give a seminar , i said to myself ,  you know this is hogwash
. why do nt you just give up this crazy idea that you re no good ?  and so i did .
how did you first get interested in the nucleolus ? in a graduate school cytology course , i had a lot of fun playing with various dyes and painting cells every color i could .
many of these dyes had a particular affinity for rna , so the region that was often most brightly lit was the nucleolus .
i m sure that there are people who , when they first saw mitochondria , went absolutely wild with excitement and expectation .
the connection between the molecular species called rna and cell structure and organization appealed to me . that s been a consistent footprint of my research .
we do some rna biochemistry and some molecular biology , but we always transport the results back to studies conducted in living cells .
nucleoli are not free - floating usually , but are anchored to the chromosomes that have the genes for ribosomal rna ( rrna ) , which in human cells are on five of the chromosomes .
you can think of them as cytological manifestations of the production of rrna and its assembly into ribosomes .
when did you first suspect that there was more going on in the nucleolus beyond ribosome synthesis ?
it was about 1986 . you begin to get a sense , or to pick things up 
we were injecting fluorescently tagged rnas into the nucleus , including various known nucleolar rnas . as a control my postdoc marty jacobson decided to try an rna that we assumed would not become localized to the nucleoli .
then we showed that five of the six srp proteins are imported into the nucleus and assemble onto srp rna in the nucleolus .
we found this in mammalian cells and it was soon confirmed by two other labs in yeast .
this really got me thinking about the possibility that the nucleolus might be doing other things as well . jumping ahead a few years , we demonstrated that , in many cells , the nucleolus seems to have a rather uncanny ability to monitor the progression of cells through interphase and to execute cell cycle arrests during nucleolar stress .
srp rna ( red ) and the pre - rrna processing enzyme fibrillarin ( green ) show distinct localizations in the nucleoli of a rat kidney epithelial cell.image  politz et al . ,
srp rna ( red ) and the pre - rrna processing enzyme fibrillarin ( green ) show distinct localizations in the nucleoli of a rat kidney epithelial cell .
we used a low concentration of the drug actinomycin , which selectively inhibits rrna transcription , to cause nucleolar stress .
well , sure , the poor cells are trying to get through g2 and all of a sudden they run out of ribosomes !  but the fact is , in almost all growing mammalian cells , ribosomes are extremely long - lived , and the short bout of drug treatment we used would have hardly made a dent in the cell s store of ribosomes .
my suspicion is that the nucleolus can sense something is wrong and it sends out a signal to stop cell cycle progression .
we were studying myoblast differentiation using a rat cell line and joan politz in my laboratory wanted to look at microrna regulation of that process .
she noted that one microrna , called mir-206 , displayed not only cytoplasmic localization , as expected , but was also prominent in the nucleoli .
we then purified the nucleoli and found they contained large amounts of four other micrornas as well .
naturally , we began to ask whether certain mrnas might visit nucleoli in order to be regulated by these micrornas . that led to the paper we just published , showing that there are a number of mrnas present in these rat myoblast nucleoli .
the mrna we chose to scrutinize , for insulin - like growth factor 2 , contained predicted binding sites not only for mir-206 , but also for the four other nucleolar micrornas .
why might certain micrornas and mrnas meet up in the nucleolus rather than the cytoplasm ?
our thinking is that there s some very important reason for keeping this mrna out of the cytoplasm until it has been pre - docked by the microrna regulating it .
this might have to do with the fact that in the nucleoplasm you have all the competing nascent mrnas and nascent micrornas . and
so the mature species get sent to the nucleolus where you do nt have any spliceosomes or nascent rnas around 
there are no contending or competing activities.this really got me thinking about the possibility that the nucleolus might be doing other things .   this really got me thinking about the possibility that the nucleolus might be doing other things .
i m going way out on a limb here and it s about ready to break .
what inspires your prolific publishing of non - research papers  book reviews , editorials , and researcher memorials ?
when i was a boy she used to show me how to write a thank - you note :  do nt blurt out  thank you for the book in the first sentence .
you need an expository sentence .  what 7-year - old knows what an expository sentence is ?
when i write a book review or a memoir , i feel like a million dollars .
i think i operate in these non - research papers in the  essay  mode , which from the latin means to try .
pederson chatting with john gurdon , a nucleolus pioneer , at the 2014 cold spring harbor meeting on nuclear organization and function.photo courtesy of cold spring harbor laboratoryyour career spans almost five decades .
what do you think were the biggest , field - altering discoveries in that time ?
pederson chatting with john gurdon , a nucleolus pioneer , at the 2014 cold spring harbor meeting on nuclear organization and function .
photo courtesy of cold spring harbor laboratory the advent of biological electron microscopy certainly gave us an appreciation of the cell that we had never had before .
also , once e. coli surrendered some of its precious secrets about molecular biology , being able to interrogate mammalian cells with the molecule in mind was really a transformation .
finally , i think the ingenious use of biological materials that exemplify or amplify a particular phenomenon has been somewhat underappreciated .
joe gall is one of my biggest scientific heroes because every one of his great discoveries  such as ribosomal gene amplification , in situ nucleic acid hybridization , and discovery of the telomeric dna repeat  came from his astute instinct for using just the right material .
pederson s career has revolved around the nucleus , nucleolus , and the nucleoproteins that inhabit them .