You will be shown an abstract from a biomedical research paper. Given this abstract, your task is to extract all unique entities of the following types: ["RNA", "CellLine", "Protein", "DNA", "CellType"].

Please return the output as a JSON object of the format: {"DNA" : ["TAL1", ...], "RNA": ["mRNAs", ...], "CellLine": ["T cells", ...], "CellType": ["monocytes", ...], "Protein": ["IL-2", ...]}. The keys should be entity types and values should be lists of extracted entities belonging to the corresponding type. If you cannot find entities belonging to a specific type, the value should be [].

Only output the JSON object and do not include any additional text.

Abstract:

The regulation of the human tumor necrosis factor alpha promoter region in macrophage, T cell, and B cell lines. The 1311-base pair human tumor necrosis factor (TNF) alpha promoter region was fused to the luciferase (Luc) reporter gene and studied in a transient transfection system in three TNF producing cell lines, the U937 macrophage cell line, the MLA 144 T cell line, and the 729-6 B cell line. This full length promoter construct can be induced by phorbol 13-myristate acetate (PMA) in each of these cell types. Analysis of a series of 5'-truncations showed several peaks of basal and PMA induced activity suggesting the presence of several positive and negative regulatory elements. A PMA responsive element was localized to a region between-95 and-36 bp relative to the transcription start site. Within this region, single AP-2-and AP-1-like consensus sequences were noted. These AP-2 and AP-1 sites were each modified with a double point mutation. A modest (20-50%) reduction in TNF promoter activity was observed with the AP-2 site mutation. However, mutation of the AP-1 site markedly diminished both the basal and PMA-activated promoter activity. Also co-transfections of the wild-type promoter construct with an AP-1 / c-jun expression vector resulted in augmented basal and PMA-induced promoter activity.
{"CellLine": ["TNF producing cell lines", "729 - 6 B cell line", "macrophage", "U937 macrophage cell line", "T cell", "B cell lines", "MLA 144 T cell line"], "CellType": [], "DNA": ["5\u0027 - truncations", "PMA responsive element", "tumor necrosis factor (TNF)", "transcription start site", "TNF promoter", "AP - 2", "AP - 1 site", "human tumor necrosis factor alpha promoter region", "negative regulatory elements", "- 95 and - 36 bp", "AP - 1 sites", "AP - 1 / c - jun expression vector", "AP - 2 site mutation", "TNF", "1311 - base pair human tumor necrosis factor (TNF) alpha promoter region", "AP - 2 - and AP - 1 - like consensus sequences", "luciferase (Luc) reporter gene", "positive", "wild - type promoter construct"], "Protein": [], "RNA": []}