You will be presented with a collection of medical randomized controlled trial (RCT) reports addressing a shared clinical question -- for instance, the efficacy of a medical intervention on a disease or condition. Given the titles and abstracts of these reports, your task is to generate the conclusion of the systematic review article synthesizing the key findings. Your conclusion should be a few sentences long. In your response, include only the conclusion and no other text. The RCT reports are below.



Title 1:
Efficacy of an Extract of Ocimum tenuiflorum (OciBest) in the Management of General Stress: A Double-Blind, Placebo-Controlled Study
Abstract 1:
A r and omized , double-blind , placebo-controlled study was conducted to evaluate the efficacy of OciBest , an extract of Ocimum tenuiflorum Linn . in symptomatic control of general stress . The participants received either placebo ( n = 79 ) or OciBest ( n = 71 ; 1200 mg of actives per day ) for six weeks . The severity of stress-related symptoms was self-evaluated by patients at weeks 0 , 2 , 4 and 6 of the trial period using a symptom rating scale . After six weeks of intervention , scores of symptoms such as forgetfulness , sexual problems of recent origin , frequent feeling of exhaustion , and frequent sleep problems of recent origin decreased significantly ( P ≤ 0.05 ) in OciBest group as compared with placebo group . Also , the total symptom scores of OciBest group revealed significant reduction ( P ≤ 0.05 ) as compared to placebo group . The overall improvement in OciBest group was found to be 1.6 times or 39 % more in the control of general stress symptoms with respect to placebo . No adverse events were reported during the study . The findings revealed that OciBest was found to be effective and well tolerated by all the patients over the six weeks of study period


Title 2:
Evidence Based Complementary and Alternative Medicine
Abstract 2:
The existing review s of massage therapy ( MT ) research are either limited to infants , adults , or were conducted prior to the publication of the most recent studies using pediatric sample s. R and omized controlled trials ( RCTs ) of pediatric MT are review ed . A literature search yielded 24 RCTs of pediatric MT  , defined as the manual manipulation of soft tissue intended to promote health and well-being in recipients between 2 and 19 years of age . Because RCTs of pediatric MT varied considerably in the amount and types of data reported , quantitative and narrative review methods were both used . Singledose and multiple-dose effects were examined separately . Among single-dose effects , significant reductions of state anxiety were observed at the first session ( g 1⁄4 0.59 , P < 0.05 ) and the last session ( g 1⁄4 1.10 , P < 0.01 ) of a course of treatment . Effects for salivary cortisol ( g 1⁄4 0.28 ) , negative mood ( g 1⁄4 0.52 ) and behavior ( g 1⁄4 0.37 ) were non-significant . Three of eleven multiple-dose effects were statistically significant . These were trait anxiety ( g 1⁄4 0.94 , P < 0.05 ) , muscle tone ( g 1⁄4 0.90 , P < 0.01 ) and arthritis pain ( g1⁄4 1.33 , P < 0.01 ) . Results of studies not permitting effect size calculation were judged to be generally consistent with quantitative results . MT benefits pediatric recipients , though not as universally as sometimes reported . Numerous weaknesses endemic to MT research ( e.g. low statistical power , frequent failure to report basic descriptive statistics ) are identified , and recommendations for future pediatric MT research are discussed


Title 3:
Controlled programmed trial of Ocimum sanctum leaf on generalized anxiety disorders.
Abstract 3:
Ocimumn sanctum , an Indian medicinal plant , has been on trial for its role in  generalized anxiety disorder ( GAD ) in hospital based clinical set-up .  Hamilton 's brief psychiatric rating scale ( BPRS ) and thorough clinical investigations were used to screen the subjects . Thirty-five subjects ( 21 male and 14 female ; average age 38.4 years ) were medicated with the plant extract in a fixed dose regime ( 500 mg/capsule , twice daily , p.o . after meal ) . They were thoroughly investigated clinical ly and using st and ard question naires based on different psychological rating scale at baseline ( day 0 ) , mid-term ( day 30 ) and final ( day 60 ) . The observations exhibited that , O. sanctum significantly ( p<0.001 ) attenuated generalized anxiety disorders and also attenuated its correlated stress and depression . It further significantly ( p<0.001 ) improved the willingness to adjustment and attention in human . Therefore , it may be concluded that O. sanctum may be useful in the treatment of GAD in human and may be a promising anxiolytic agent in near future


Title 4:
Prevention of insulin resistance by ingesting aqueous extract of Ocimum sanctum to fructose-fed rats.
Abstract 4:
The study was aim ed to examine if oral administration of the aqueous extract of the whole plant OCIMUM SANCTUM ( OS ) protects against the development of  insulin resistance in fructose fed rats . Male Wister rats were r and omly divided into four groups of eight animals each : group-S ( starch diet ) , group-F ( fructose diet ) , group-F+OS ( fructose diet along with OCIMUM SANCTUM extract at a dose of 200 mg/kg ) , group-S+OS ( starch diet along with OCIMUM SANCTUM ) . During the experimental period of 60 days body weight , plasma glucose , insulin , and triglycerides were measured at an interval of 15 days . Insulin sensitivity was assessed at the end of experimental period by measuring glucose-insulin index , which is the product of the areas under the curve of glucose and insulin during oral glucose tolerance test . The nontoxic nature of OS was revealed by unaltered body weight , plasma glucose , insulin , and triglyceride levels in group-S+OS when compared with group-S. A significant gain in body weight , hyperglycemia , hyperinsulinemia , hypertriglyceridemia , and insulin resistance were observed in group-F when compared with group-S. OS treatment prevented the observed fructose induced alterations in group-F+OS . In conclusion , our results suggests that oral administration of OS aqueous extract could delay the development of insulin resistance in rats and may be used as an adjuvant therapy for treating diabetic patients with insulin resistance


Title 5:
Double-blinded randomized controlled trial for immunomodulatory effects of Tulsi (Ocimum sanctum Linn.) leaf extract on healthy volunteers.
Abstract 5:
ETHNOPHARMACOLOGICAL RELEVANCE Tulsi ( Ocimum sanctum Linn . ) is considered as a sacred herb and traditionally it is believed that consumption of Tulsi leaf on empty stomach increases immunity . Experimental studies have shown that alcoholic extract of Tulsi modulates immunity . MATERIAL S AND METHODS The present study was design ed to evaluate the immunomodulatory effects of ethanolic extract of Tulsi leaves through a double-blinded r and omized controlled cross-over trial on healthy volunteers . Three hundred milligrams capsules of ethanolic extracts of leaves of Tulsi or placebo were administered to 24 healthy volunteers on empty stomach and the results of 22 subjects who completed the study were analyzed . The primary objective was to study the levels of Th1 and Th2 cytokines ( interferon-γ and interleukin-4 ) during both pre and post intervention period in blood culture supernatants following stimulation with lipopolysaccharide and phytohaemagglutinin . Other immunological parameters such as T-helper and T-cytotoxic cells , B-cells and NK-cells also were analyzed using Flowcytometry . RESULTS Statistically significant increase in the levels of IFN-γ ( p=0.039 ) , IL-4 ( p=0.001 ) and percentages of T-helper cells ( p=0.001 ) and NK-cells ( p=0.017 ) were observed after 4 weeks in the Tulsi extract intervention group in contrast to the placebo group . CONCLUSIONS These observations clearly ascertain the immunomodulatory role of Tulsi leaves extract on healthy volunteers


Title 6:
Immunotherapeutic potential of Ocimum sanctum (L) in bovine subclinical mastitis.
Abstract 6:
Immunotherapeutic potential of aqueous extract of Ocimum sanctum ( O. sanctum ) leaf in bovine sub- clinical mastitis ( SCM ) was investigated . Somatic cell count ( SCC ) , total bacterial count ( TBC ) , milk differential leukocyte count ( DLC ) , phagocytic activity and Phagocytic index and leukocyte lysosomal enzymes like myeloperoxidase and acid phosphatase content were evaluated after intramammary infusion of aqueous leaf extract of O. sanctum . The results revealed that the aqueous extract of O. sanctum treatment reduced the TBC and increased neutrophil and lymphocyte counts with enhanced phagocytic activity and phagocytic index . Similarly , the lysosomal enzymes contents of the milk polymorphonuclear cells ( PMNs ) were also enhanced significantly in animals treated with the extract . The results suggest that the crude aqueous extract of O. sanctum ( leaf ) possesses some biologically active principles that are antibacterial and immunomodulatory in nature . As such , the present wok substantiates the therapeutic use of medicinal herb and also emphasizes on the potential of the commonly available non-toxic substances to enhance the mammary immunity


Title 7:
Diversity of the genus Ocimum (Lamiaceae) through morpho-molecular (RAPD) and chemical (GC–MS) analysis
Abstract 7:
In this present study , we have described the diversity of nine Ocimum genotypes naturally grown in the Dakshin Dinajpur district of West Bengal , India . Their diversity was determined on the basis of morphological , chemical and r and omly amplified polymorphic DNA ( RAPD ) to determine the level of variation present in the genus Ocimum . Among nine Ocimum genotypes six ( O. americanum , O. × africanum , O. basilicum , O. gratissimum , O. kilim and scharicum  and  O. tenuiflorum )  are found to be different Ocimum species and the rest are as varieties . A total of 18 qualitative and 17 quantitative morphological traits and chemical compositions were evaluated . Significant variations were observed in the morphological traits except O. × africanum and O. basilicum species . Cluster generated from the morphological data showed two different groups viz . basilicum group and sanctum group . Chemical analysis did not show much variation between morphologically similar species viz . O. × africanum and O. basilicum . However , RAPD analyses clearly showed that O. × africanum and O. basilicum are different species . Thus the combined analyses of morphological traits , chemical and molecular markers represent the best possible approach to confirm taxonomic delineation . Moreover , we are reporting O. × africanum for the first time from this region as well as from West Bengal , India
The review ed studies reinforce traditional uses and suggest tulsi is an effective treatment for lifestyle-related chronic diseases including diabetes , metabolic syndrome , and psychological stress .