You will be presented with the abstract, introduction, and discussion section from a biomedical research article. Your task is to create a summary that a layperson can understand, capturing the essence of the research article. Keep essential scientific terms, but ensure that the language remains clear and the concepts are explained in an uncomplicated manner.

Title: ABCC5, a Gene That Influences the Anterior Chamber Depth, Is Associated with Primary Angle Closure Glaucoma}
Article:
Abstract:
Anterior chamber depth( ACD) is a key anatomical risk factor for primary angle closure glaucoma( PACG). We conducted a genome-wide association study( GWAS) on ACD to discover novel genes for PACG on a total of 5, 308 population-based individuals of Asian descent. Genome-wide significant association was observed at a sequence variant within ABCC5( rs1401999; per-allele effect size = −0. 045 mm, P = 8. 17×10−9). This locus was associated with an increase in risk of PACG in a separate case-control study of 4, 276 PACG cases and 18, 801 controls( per-allele OR = 1. 13[95% CI: 1. 06–1. 22], P = 0. 00046). The association was strengthened when a sub-group of controls with open angles were included in the analysis( per-allele OR = 1. 30, P = 7. 45×10−9; 3, 458 cases vs. 3, 831 controls). Our findings suggest that the increase in PACG risk could in part be mediated by genetic sequence variants influencing anterior chamber dimensions.
Introduction:
Primary angle closure glaucoma( PACG) remains a major cause of irreversible blindness, particularly in Asian countries such as China[1], Mongolia[2], Singapore[3], and India[4] with up to 80% of the estimated 15 million people afflicted with PACG resident in Asia[5]. We recently conducted a genome-wide association study( GWAS) on PACG with 3, 771 PACG cases and 18, 551 controls, and identified 3 strongly associated genetic variants: rs11024102 in PLEKHA7, rs3753841 in COL11A1 and rs1015213 located between PCMTD1 and ST18 on Chromosome 8q[6]. As these 3 sequence variants only explained <2 percent of PACG risk, we looked into other methodologies besides the GWAS based approaches to identify more genes that underlie PACG susceptibility. The clinical heterogeneity of PACG suggests that disease-related endophenotypes/quantitative traits may help elucidate true disease genes. Quantitative phenotypes allow individuals to be viewed along the continuum of risk, and may provide additional information which could complement dichotomous measures of affection status[7],[8]. Such an approach has been used in the study of genetic variants controlling lipid traits and susceptibility to coronary artery disease[7],[8]. Smaller anterior segment dimensions are a hallmark of PACG, with shallower anterior chamber depth( ACD), the cardinal feature associated with increased susceptibility to PACG[9],[10]. Eyes with an ACD of less than 2. 80 mm were more inclined to have angle closure when compared to eyes with an ACD of at least 3 mm( odds ratio( OR), 42. 5; 95% confidence interval( CI), 27. 4–66. 2)[11]. There is also a greater likelihood of developing glaucomatous optic neuropathy in persons with the shallowest anterior chambers[12]. ACD, an easily and precisely quantified measure by ocular imaging techniques, is a normally distributed quantitative trait within the general population. It displays high heritability with a coefficient as high as 0. 90[13],[14], and can be considered an endophenotype for PACG. To identify genetic variants that significantly influence ACD, and to determine if such genes( if any) affects PACG risk, we conducted a two-staged study, first a GWAS on a total of 4, 484 population-based individuals of Indian and Malay ethnicity from Singapore, and Chinese from Beijing, China. Secondly, the identified QTLs for ACD were assessed for association in PACG case cohorts.
Discussion:
In this study, a GWAS of ACD in Singaporean Indians and Malays and Chinese from Beijing China found rs1401999 within ABCC5 to contribute to the normal variation of ACD, a quantitative trait relevant to PACG. Interestingly, none of the previously identified PACG-associated genetic variants[6] were significantly associated with ACD[18]. We also demonstrated the association of rs1401999 with PACG using case-control cohorts from multiple populations across Asia. Importantly this association surpassed GWAS significance when the analysis was confined to control pre-selected to have open angles. ABCC5, also known as multidrug resistance protein 5( MRP5), has been shown to participate in tissue defense and cellular signal transduction through efflux of anticancer drugs, toxicants and a second messenger cGMP[19].[20],[21]. It is expressed in most human tissues, including the cornea[22], retinal pigment epithelium and retina of the eye[23]. We also noted ABCC5 expression in ocular structures relevant to PACG such as the iris, ciliary body, and lens. However, its exact role in the context of PACG is not yet known. The significant association between ABCC5 rs1401999 with a shallower ACD argues favorably for a role in eye growth, particularly that of the anterior segment. Intriguingly, a study in zebrafish suggested that Abcc5 may play an active role in eye development through the regulation of intracellular cGMP levels. Zebrafish Abcc5, which shares 73% amino acid sequence identity with human ABCC5, is highly expressed in the lens of the developing eye[24]. Notably, the blockage of endogenous ABCC5 activity by its dominant-negative was shown to retard development, producing smaller eyes as well as overall reduction of body length and pigmentation of embryos[24]. Abcc5 knockout mice have been generated but an evaluation of their eyes was not reported[25]. A developmental role for ABCC5 in mammalian eyes therefore remains to be defined and will require further detailed studies in model organisms. In addition, the linkage disequilibrium( LD) block that includes rs1401999 and ABCC5, also includes the presenilin-associated rhomboid-like( PARL) gene, 5-hydroxytryptamine receptor 3D( HTR3D) gene and the 5-hydroxytryptamine receptor 3C( HTR3C) gene( Figure S6). It is thus possible that rs1401999 might simply be in LD with an as yet unidentified causal variant, and it remains unclear whether the causal alleles or group of alleles influence ABCC5 or any of the neighboring genes to influence ACD. However, the clear expression within ocular tissues and a possible role in eye development make ABCC5 a rather attractive candidate gene for ACD. Indeed, re-sequencing of the region will be necessary to identify novel potentially functional polymorphisms related to PACG pathogenesis. The prevalence of the pre-cursor stage of PACG, namely narrow angles, is about 10% in many Asian populations[3],[17]. Given the association between angle closure and shallow ACD[11] it is therefore appropriate to remove the ‘at-risk individuals’ from the control population in order to assess the true relationship between ACD QTLs and PACG. Unsurprisingly, the evidence of association between rs1401999 and PACG was augmented when we only included sample collections where the controls had definite open angles. Similar observations have also been seen with very recent studies on Alzheimer's disease, where the inclusion of general population-based controls resulted in significant underestimation of the odds ratio conferred by the disease-associated SNP compared to when properly matched, risk-free controls were applied,[26].[27],[28]. We caution that the modest statistical evidence reported here for rs1401999, when examined in a total of 4, 276 PACG cases and 18, 801 controls, is at least 5 orders of magnitude below that of the three PACG-associated variants from our recent GWAS study[6]. Clinical studies have shown that ACD is only a modest determinant of angle width[29]. Therefore, this may explain why an ACD controlling gene such as ABCC5 would only confer a relatively small effect on PACG disease itself. Incidentally, the proportion of PACG risk explained by ABCC5 rs1401999 is 0. 35%( 95% confidence interval = 0. 01 to 1. 2%). Our study highlights the fact that even larger sample sizes may be necessary to dissect and conclusively identify the possible modifiers of genetic risk conferred by variants of modest effects, particularly when they exert their action on disease pathogenesis via endophenotypes. The following observations can also be drawn from this study. It is important that all sample collections are included and assessed transparently especially when drawn from diverse populations. Secondly, despite the broad-based success in the use of large numbers of unselected, population-based controls in genetic studies[30],[31],[32],[33],[34],[35] the deployment of controls with proper clinical phenotyping and documentation will often assist in more definitive identification of susceptibility genes. A comprehensive examination of all variation around ABCC5 using targeted deep re-sequencing is now necessary to parse the true association signal in an effort to more completely understand the role of this gene in ACD and PACG. In summary our study identified a common genetic variant within ABCC5 as being significantly associated with ACD, which was also associated with a modest risk of PACG. Our findings are largely in keeping with the anatomical risk factors of individual susceptibility to PACG in the eye, whereby shallower ACD is a cardinal clinical and pathogenic feature, predisposing the eye to more ‘crowded’ anterior segment and thus increasing the risk of PACG. Our study provides further clues to genetic mechanisms underlying this major global cause of blindness.
The anterior chamber is the space within the eye which is bound by the cornea , and the anterior surfaces of the iris and lens . Anterior chamber depth ( ACD ) is the distance measured along the eye's optical axis , from the cornea to the lens surface . ACD is an important risk factor for primary angle closure glaucoma ( PACG ) , a major cause of irreversible blindness worldwide , and in particular , individuals of Asian ethnicity . In order to identify the genes that underlie PACG susceptibility , we conducted a two-staged study . We first conducted a large scale genetic study on a total of 5 , 308 population-based individuals of Asian descent to identify the genetic variants that influence ACD . This was followed by testing for associations between the identified genetic variant and PACG in another independent collection of 4 , 276 PACG cases and 18 , 801 controls . We found that a genetic variant within ABCC5 was associated with an increased risk of having PACG . Our findings suggest that the increase in PACG risk could in part be mediated by genetic sequence variants that influence the anterior chamber dimensions of the eye .