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any variation in root canal morphology should be recognized before or during root canal treatment .
an untreated canal , which a clinician fails to detect , may be a cause of failure .
various methods to assess the internal anatomy of root canal systems are : ( i ) conventional radiographs ( ii ) canal staining and tooth clearing ( iii ) plastic resin injection ( iv ) digital and contrast medium enhanced radiographic techniques ( v ) sectioning ( vi ) in vitro microscopic examination ( vii ) computed tomography ( ct ) techniques ( vii ) micro - ct and ( viii ) cone beam ct ( cbct ) .
cbct was developed in the late 1990s by italian and japanese groups to produce undistorted three - dimensional images of the teeth and their surrounding tissues .
it helps in visualizing the number of root canals and their convergence or divergence from each other .
the principle goal of endodontic therapy is to retain every functional component of the dental arch , including third molars .
when reviewing the literature regarding root and canal morphology using the pub med database ( national library of medicine ) , there were no studies on the root canal morphology utilizing cbct images of permanent maxillary third molar in indian population .
the aim of this study was to investigate the root canal morphology of maxillary third molars and to evaluate the number of roots and canals and classify them according to vertucci .
9000c 3d , 70 kvp , 10 ma , 36 s scan time , 10.8 s exposure time , 5 cm diameter5 cm height scan volume , france ) images of 325 patients who presented at a private clinic for various dental problems in bhopal between june 2011 and march 2015 was done .
all cbct image was observed and evaluated for the presence and absence of maxillary third molars .
the scans were produced by a technician according to the manufacturer 's recommended protocol using a minimum field of view of 40 mm  40 mm or 60 mm  60 mm and lowest dose radiation .
the cbct images were analyzed with the inbuilt cs3d imaging software ( nnt ) in a hp workstation ( hp compaq le 1911 ) with a 19 inch hp led screen with a resolution of 1280  1024 pixels on a dual monitor .
the images were visually enhanced by adjusting contrast , brightness and grey scale using the image processing tool in the software to ensure better visualization .
three endodontists and a trained technician concurrently evaluated all the images to reach an agreement in the interpretation of the radiographic findings .
type of canal configuration , presence of extra root and numbers of canals were evaluated using the nnt toolbar by carefully rolling downward through the images from the pulp chamber to the root apex at the axial tomographic slices .
the images were rotated in the entire axis , and slices were taken in all the three planes , coronal , sagittal , and axial planes to confirm the findings .
( ii ) third molars with no periapical lesions ; ( ii ) no endodontic treatment performed ( ii ) no root canals with open apices , resorption or calcification ; and ( iii ) the cbct images of good quality .
the teeth with root canal fillings , restorations , posts , or crowns were excluded from the study .
the final sample group included data from 116 patients ( 66 males and 50 females ) .
following information from the individual images was recorded ( i ) the number of roots and their morphology ; ( ii ) the number of canals per tooth ; ( iii ) the canal configuration in each root using vertucci 's classification ; and ( iv ) the frequency of additional roots and the frequency of c - shaped canals in the third molars . frequency and percentage of variables were calculated using statistical package for social sciences ( spss v.21.0 , ibm , armonk , new york ) version 21.0 and data was further analyzed by pearson chi - square test and fisher 's exact test .
56.9% of the teeth belong to males , and 43.1% were of females , 43.1% were of right side , and 56.9% were of the left side .
maximum maxillary third molars ( n = 116 ) had three root ( 55.2% ) followed by one root ( 31.0% ) and two roots ( 13.0% ) .
analysis of number of canals revealed that maximum number of maxillary third molars had 3 canals ( 37.9% ) followed by 04 canals ( 24.1% ) and 1 canal ( 19% ) , 3.4% of teeth had 5 canals .
in single rooted maxillary third molar , vertucci type i ( 47.1% ) was predominant .
most of the double - rooted teeth have vertucci type i ( 42.9% ) and type iv ( 42.9% ) in buccal root and type i in the palatal root ( 62.5% ) .
mesiobuccal root of three - rooted maxillary third molars had type i ( 43.8% ) and type iv ( 40.6% ) , distobuccal root and palatal root had type i ( 87.5% and 100% ) , respectively [ table 1 ] .
vertucci classification of canals in maxillary third molars comparison of vertucci classification revealed that type i was prevalent in both males and females in single rooted maxillary third molars , and the difference was statistically significant .
the percentages of other vertucci types in males were type iv ( 14.3% ) , v ( 28.6% ) , and vi ( 14.3% ) and in females were type ii ( 30.0% ) and type iii ( 20.0% ) . in double - rooted teeth ,
buccal root had type i ( 75% ) predominant in males as compared to females where type iv ( 66.7% ) was predominant .
in distobuccal root , vertucci type i was predominant in both males ( 85.7% ) and females ( 90.9% ) [ table 2 ] .
vertucci classification of canals in maxillary third molars according to gender when vertucci classification of canals in maxillary third molars was compared between the right and left side of the oral cavity , it showed that in single rooted teeth type i was predominant in both the sides .
type v classification was observed in 28.6% of right side single rooted teeth whereas in left side 20% were type iii , 10% were and type iv .
chi - square test showed a significant difference between the right and left side of the oral cavity . in double - rooted maxillary third molar , vertucci type i and
type iv was predominant in left side whereas on the right side type i , type ii , and type iv were equally present , and the difference was not statistically significant . in three - rooted maxillary third molars in mesiobuccal root ,
vertucci type iv ( 46.7% ) was predominant on right side as compared to left side whereas type i ( 58.8% ) were predominant , and the result was statistically significant ( p = 0.013 ) . in distobuccal root on both sides ,
these variations in the root canal morphology are thought to be racially and genetically determined .
therefore , it is necessary to investigate variations in tooth anatomy and their characteristic features in different racial groups .
the multiplanar cbct scans obtained from axial sections of 116 maxillary third molars revealed that frequency of three - rooted maxillary third molars was maximum ( 55.2% ) followed by one root ( 31.0% ) [ figure 1 ] and two roots ( 13.8% ) .
similar results were observed in burmese population by clearing technique where 51% of teeth had three separate roots , and 49% had fused roots . a study conducted by ng et al . in the same population using canal staining and tooth clearing technique showed 25% had three roots , and 31% had fused roots . cone beam computed tomography image showing two canals in single rooted third molar in the present study , maximum maxillary third molars showed 3 canals ( 37.9% ) followed by 4 canals ( 24.1% ) [ figures 2 and 3 ] and 1 canal ( 19% ) .
the majority of palatal ( 100% ) and distobuccal ( 96% ) roots possessed one canal ( type i ) . according to literature ,
vertucci type i was more common in distobuccal root ( 98.1% ) and palatal root ( 100% ) .
cone beam computed tomography image of maxillary third molar showing vertucci v configuration cone beam computed tomography image showing maxillary third molar with mb2 mesiobuccal root shows considerable morphological variations in vertucci classification . a conventional radiograph can detect only 55% of mb2 due to the superimposition of anatomic structures in a buccolingual plane which fail to detect small structural density changes .
stropko found 20.0% of third molars had mb2 , whereas it was 37% in a study done by green .
the prevalence of mesiobuccal roots with two canals molars was 39% with vertucci type ii and type iv configurations .
study conducted on maxillary molars in south asian indian population using clearing technique showed that mesiobuccal roots of maxillary third molars exhibited 57.4% had type i , 32% had type ii , 2.1% had type iii , 8.5% had type iv , and 1% had type v canal anatomy . in this study , single rooted maxillary third molars , the palatal root of double - rooted maxillary third molars , and all three roots of a triple rooted maxillary third molars
in buccal root of double - rooted maxillary teeth , the most common type was vertucci type i and type iv .
a similar retrospective study in mandibular molars in a turkish population using cbct showed that majority of mandibular molars were two rooted with three canals .
mesial roots had more complex canal systems with more than one canal , whereas most distal roots had a type i configurations .
gender predilection for the prevalence of vertucci type showed that vertucci type i was predominant in single rooted teeth in both males and females .
topological predilection of vertucci classification revealed that three rooted maxillary third molars showed mesiobuccal root vertucci type iv ( 46.7% ) predominant on right side in mesiobuccal root as compared to left side whereas type i ( 58.8% ) were predominant and the result was statistically significant ( p = 0.013 ) .
prevalence of c - shaped canals in maxillary third molars was 3.4% in this study [ figures 4 and 5 ] . according to sidow et al .
, 4.67% were identified in maxillary third molars ( all of them were double - rooted ) .
cone beam computed tomography image showing c - shaped canal in maxillary third molar cone beam computed tomography image of sagital view of c - shaped canal cbct offer high - resolution isotropic images which helps in identification of canals which is not possible in conventional radiograph if canal cortication is lost .
it offers three - dimensional geometric accuracy compared with conventional radiographs without superimposition of anatomical structures where data can be reoriented in their true spatial relationships .
however , consideration to use cbct should only be done when the conventional periapical radiographs fail to provide adequate information about the area of interest , as cbct views may also show some misleading findings .
there was a high prevalence of three - rooted maxillary molars with three canals in central india population .
vertucci type i classification was most common in single rooted maxillary molars , distobuccal roots and palatal roots of three - rooted maxillary third molars .
objective : the aim of this study was to investigate the root and canal morphology of maxillary third molars in central india population using cone - beam computed tomography ( cbct ) analysis.materials and methods : cbct images of 116 maxillary third molars were observed , and data regarding the number of roots , the number of canals , and vertucci 's classification in each root was statistically evaluated.results:majority of maxillary third molars had three roots ( 55.2% ) and three canals ( 37.9% ) . most mb root ( 43.8% ) , db root ( 87.5% ) , and palatal root ( 100% ) of maxillary third molars had vertucci type i. mesiobuccal root of three - rooted maxillary third molars had vertucci type i ( 43.8% ) and type iv ( 40.6% ) configuration 
 . overall prevalence of c - shaped canals in maxillary third molars was 3.4%.conclusion : there was a high prevalence of three - rooted maxillary molars with three canals .