You will be shown an abstract from a biomedical research paper. Given this abstract, your task is to extract all unique entities of the following types: ["RNA", "CellLine", "Protein", "DNA", "CellType"].

Please return the output as a JSON object of the format: {"DNA" : ["TAL1", ...], "RNA": ["mRNAs", ...], "CellLine": ["T cells", ...], "CellType": ["monocytes", ...], "Protein": ["IL-2", ...]}. The keys should be entity types and values should be lists of extracted entities belonging to the corresponding type. If you cannot find entities belonging to a specific type, the value should be [].

Only output the JSON object and do not include any additional text.

Abstract:

A nuclear factor NF-GM2 that interacts with a regulatory region of the GM-CSF gene essential for its induction in responses to T-cell activation: purification from human T-cell leukemia line Jurkat cells and similarity to NF-kappa B. Activation of T cells by antigen, lectin, or a combination of phorbol-12-myristate acetate (PMA) and calcium ionophore (A23187) leads to the induction of genes for a set of lymphokines, including granulocyte-macrophage colony-stimulating factor (GM-CSF). We demonstrated in earlier studies that the upstream region of the mouse GM-CSF promoter at positions between-95 and-73 is essential for transcriptional activation in response to PMA / A23187. This region contains two DNA-binding motifs, GM2 and GC-box. The GM2 sequence (GGTAGTTCCC) is recognized by an inducible factor NF-GM2; the other (CCGCCC) by constitutive factors A1, A2, and B. To elucidate the mechanism of GM-CSF gene activation, we have purified the inducible factor NF-GM2 from the nuclear extract of stimulated Jurkat cells on the basis of specific DNA-binding activity. The purified NF-GM2 consists of 50 (p50) and 65 kDa (p65) polypeptides and has a binding activity specific for both the GM-CSF and immunoglobulin kappa (GGAAAGTCCC) enhancers. Electrophoretically purified p50 alone can form a protein-DNA complex, but in the mixture, p50 associates preferentially with p65 to form the NF-GM2 complex. In addition, p65 gave per se, with low affinity, a protein-DNA complex that migrated more slowly than native NF-GM2 complex. Furthermore, an antiserum against KBF1 (identical to 50 kDa NF-kappa B protein) reacted with the p50 of NF-GM2, indicating that the NF-GM2 polypeptide can not be immunologically differentiated from the 50 kDa subunit of NF-kappa B. The purified NF-GM2 activated in vitro transcription from the kappa B enhancer, while it failed to stimulate transcription from the GM-CSF promoter harboring the GM2 sequence. This suggests that the activation mechanism of the GM-CSF gene through the GM2 / GC-box sequence is different from that of genes carrying the kappa B enhancer alone.
{"CellLine": ["T - cell leukemia line Jurkat cells", "T cells", "Jurkat cells"], "CellType": ["T cells"], "DNA": ["- 95 and - 73", "GC - box", "regulatory region", "upstream region", "GM - CSF gene", "mouse GM - CSF promoter", "GM2 sequence", "other", "immunoglobulin kappa (GGAAAGTCCC) enhancers", "GM - CSF promoter", "GM2 / GC - box sequence", "kappa B enhancer", "DNA - binding motifs", "GM2"], "Protein": ["50 kDa NF - kappa B protein", "p65", "NF - GM2 polypeptide", "constitutive factors", "p50", "inducible factor NF - GM2", "NF - GM2 complex", "A1", "B", "GM2", "lectin", "protein - DNA complex", "50 kDa subunit", "NF - kappa B", "nuclear factor", "KBF1", "lymphokines", "GM - CSF", "A2", "NF - GM2", "granulocyte - macrophage colony - stimulating factor"], "RNA": []}