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in this study , which was conducted from may 2008 to september 2009 in shahrekord ( iran ) , the existence of h. pylori was investigated in the serum , fresh raw milk and faeces of 92 lactating holstein cows from 12 flocks ( size range 3040 cows ) .
cows were selected according to lactation and age ( at least two years old ) . there were no differences in the environmental situa - tions or the feeding and sanitary management of the flocks .
five millilitres of blood , 10 millili - tres of milk and 10 grams of faeces from each cow was collected into sterile common bottles .
the specimens were transported in ice - cooled containers to the laboratory within two hours after collection .
we used elisa to investigate igg antibody titre against h. pylori in the serum samples7 according to the manufacturer 's instructions ( radim co. , iran ) .
milk and faeces samples of seropositive cows were tested for h. pylori antigens using the elisa kit ( 11 ) ( ga co. germany ) .
the helicobacter pylori igg antibody and antigen test is based on an enzyme immunoassay ( elisa ) utilising a horseradish peroxidase conjugated detection antibody or antigen and the results were read by spectrophotometer ( 450 nm ) .
the cut - off values were : less than 0.140 was considered negative ; between 0.140 and 0.159 was considered equivocal and greater than or equal to 0.160 was considered positive .
the results were then confirmed by a pcr test.12 dna was extracted by the dna extraction kit ( roche co. germany ) and its density was assessed by optic densitometry .
extracted genomic dna was amplified for the urec gene and detected with the specific primers hp - f : 5-gaataagcttttaggggtgttagggg-3 , hp - r : 5gcttactttctaacactaacgcgc-3. the gene product was 294 bp .
pcr reactions were performed in a final volume of 50 l containing 5 l 10 buffer + mg , 2 mm dntp , 2 unit taq dna polymerase , 100 ng genomic dna as a template , and 25 picomole of each primer .
pcr was performed using a thermal cycler ( eppendorf co. germany ) under the following conditions : an initial denaturation for 10 minutes at 94c ; 35 cycles for 1 minute at 94c , 1 minute at 55c , 1 minute at 72c , and a final extension at 72c for 10 minutes.12 pcr yields were electrophoresed in 1.5% agarose gels ( roche co. germany ) containing ethidium bromide . a dna ladder ( fermentas co. germany )
based on serological tests ( elisa ) , it was found that out of 92 serum specimens , 25 ( 27% ) of the cows were positive for the h. pylori igg antibody and 67 specimens were negative . from these 25 seropositive cows 10 ( 40% ) faeces samples and four ( 16% ) milk samples were antigen positive for h. pylori ( table 1 ) .
the urec gene was detected in the milk and faeces samples of antigen positive cows using the pcr method .
the seropositive cows came from six different flocks and the faeces and milk antigen - positive samples came from three of these .
the prevention and control of the prevalence of h. pylori infection and thereby the spread of gastritis , gastric ulcer , and gastric cancer in humans is very important . in central iran ,
one of the major sources of infection in humans could be cow , sheep , and goat 's milk contaminated with h. pylori.10 the consumption of milk and its products vary considerably in different regions in the world .
cow 's serum , milk , and faeces were investigated to find the possible source of contamination . for this purpose
, the antibody against h. pylori was investigated in 92 cows from 12 similar flocks .
the results showed that 27% of serum samples from six flocks showed antibody against h. pylori .
the existence of h. pylori antibody in cow 's serum might indicate that h. pylori is a commensal in cows , which might be h. pylori 's natural host as dore et al .
indicated for sheep.7 seropositive cows were tested for h. pylori antigen in faeces and milk .
forty per - cent16 of faeces specimens and 16% ( four ) raw milk specimens were found to be antigen positive for h. pylori from three flocks .
the cost - efficient elisa kit used for the detection of h. pylori igg antibody is very sensitive ( 96% ) and highly specific ( 97% ) and for h. pylori antigen detection , sensitivity of the test is 98% and its specificity is 99% .
the existence of urec gene was also confirmed in positive antigen samples of milk and faeces by pcr method .
fujimura et al showed that the prevalence of h. pylori was 50% in cow faeces and 38% in soil samples .
milk could also be contaminated during production or because of the inadequate post - processing hygienic management of the product , which could transmit the bacteria to humans.9 azevedo et al could not prove the existence of h. pylori in milk through culturing .
these findings are very important to explain the way of transmission of h. pylori to humans through milk and food.13 fujimura et al reported that h. pylori might not be cultured in pasteurised cow 's milk because it might be changed to a coccoid form through pasteurisation .
they found the urea gene of h. pylori in 13 out of 18 ( 72.2% ) raw milk samples and in 11 out of 20 ( 55% ) commercial pasteurised milk samples.9 wang et al and orozco et al showed a progressive decrease in bacterial load with an average survival of nine days in pasteurised milk and 12 days in uht milk , with an estimated average of original inoculums of 105 and 106 cfu / ml .
other studies were unable to pick up h. pylori from the culture of pasteurized milk .
anti h. pylori effects in milk might damage h. pylori and inactivate a non culturable state that inhibits its multiplication in culture media.1415 h. pylori changes in three different forms under environmental stress : first , viable spiral forms that are culturable , virulent , and infectious and induce inflammation in experimental animals ; second , in viable coccoid forms that are nonculturable , less virulent , and less likely to colonise and induce inflammation in experimental animals ; and a third form that is a nonviable degenerative forms of dying h. pylori.16 it is necessary to detect the coccid form of active bacteria by using the pcr method.17
this study is the first to look for the presence of bacterial antigens in cow 's milk , one of the most consumed foods for children in iran , to recognize the mode of transmission of h. pylori .
according to this study and similar previous ones , faecal contamination and consuming infected cow milk might be a transmission way for h. pylori infection and unfit management could transfer h. pylori to humans .
the awareness of h. pylori epidemiology and methods of its distribution is essential for public health authorities and controlling the spread of this bacterium .
further investigation with a greater number of samples is necessary to verify the ability of h. pylori to spread via milk consumption .
the principal idea was from hghs who desigened and coordinated the study also participated in writing and editing the manuscript .
ar provided assistance in the design of the study and conducting , assessments and coordination of the study .
az and ar were the manager of the study and participated in designing , conducting the study and assessments .
background : the prevalence of helicobacter pylori infection , which may increase the risk of gastritis , peptic ulcers , and cancer , has increased worldwide . 
 this number is estimated to be around 7090% in developing countries and 2550% in developed countries . 
 it is possible that the bacterium can be transmitted via food and water as well as zoonotically and iatrogenically . 
 because of high prevalence of this infection in iran , the aim of this study is to examine whether h. pylori infection might be transmitted from cow 's milk and faeces.methods:the existence of the h. pylori antibody and antigen was investigated in samples of serum , milk , and faeces from 92 lactating holstein cows in shahrekord , iran . 
 the h. pylori antigen and antibody were detected using elisa and were confirmed by pcr.results:it was found that out of 92 serum specimens , 25 ( 27% ) of the cows were positive for the h. pylori antibody and 67 specimens were negative . from these 25 seropositive cows , 
 10 ( 40% ) faeces samples and four ( 16% ) milk samples were antigen positive for h. pylori . 
 four of the antigen - positive milk specimens were also antigen positive for faeces . 
 the existence of the urec gene was also confirmed in positive samples of milk and faeces.conclusions:there is a possibility that cow 's milk is a transmission mode in h. pylori infection and faecal contamination and inappropriate management processes could transfer h. pylori to humans . 
 the awareness of the h. pylori epidemiology and its method of distribution are necessary for public health measures and controlling the spread of this bacterium . 
 further investigation with a greater sample number is necessary to verify the ability of h. pylori transmission via milk consumption .