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Paper text:
one of the most contentious issues related to lyme disease has been whether infection with b. burgdorferi can be easily eradicated by conventional antimicrobial treatment or whether it is a persistent , recurrent and difficult to eradicate infection .
post - treatment lyme disease syndrome refers to patients with persistent , non - specific symptoms , such as arthralgia , fatigue or perceived cognitive impairment 6 months after completion of treatment for lyme disease .
many of those who believe in a condition termed  chronic lyme disease  argue that documenting that the bacteria can survive a standard course of antimicrobial treatment will prove that chronic lyme disease and post - treatment lyme disease syndrome are indeed a consequence of persistent infection with b. burgdorferi .
this belief persists , despite a mountain of scientific evidence that chronic lyme disease , a label without a case definition that is applied to patients with non - specific symptoms more properly designated  medically unexplained symptoms  , does not exist [ 13 ] .
several studies in which investigators have claimed to have documented persistence of b. burgdorferi in humans , despite treatment with antimicrobials , have not been reproduced or have been shown to be due to laboratory contaminants [ 48 ] . some studies in which models of the infection in animals ( usually in either mice or non - human primates ) have been performed have lent support to the argument that b. burgdorferi can persist in tissues despite antimicrobial treatment . however , there are numerous problems with the animal models that have led many to question either the reliability of the results of these studies or the generalizability of the results to disease in humans . among the many problems with animal models
is the difficulty of mimicking the route and infectious dose of b. burgdorferi in human infection , differences in the pharmacokinetic and pharmacodynamic parameters of antimicrobial treatment in the animals vs. humans , and differences in immune responses of different animal species ( for example , mice are reservoirs for b. burgdorferi in nature ) .
in addition , even in the rare instances in which b. burgdorferi has been identified in treated animals , it has not been shown to be viable ( that is , able to replicate in culture media ) . moreover , whether it is producing symptomatic disease in these animals is speculative .
several recent studies have shed additional light on this topic and have added to the evidence that viable b. burgdorferi do not persist after antimicrobial treatment of humans .
it is well recognized that subsequent episodes of erythema migrans are not unusual in patients with an initial episode who receive prompt antimicrobial treatment .
if b. burgdorferi persist despite antimicrobial treatment , it would make sense that subsequent episodes of erythema migrans might be due to the originally infecting strain of the organism . in a study to assess whether such episodes were new infections or recurrences , strains of b. burgdorferi from 17 patients with two or more episodes of culture - proved erythema migrans had 22 paired episodes with organisms available for analysis ( first and second episodes , and , for those with additional episodes , third and fourth episodes ) .
strains were compared by amplifying their dna with polymerase chain reaction ( pcr ) assay of each strain , followed by genotyping of outer surface protein c of each strain . in all instances ,
the strains in each of the paired episodes were different , indicating that each of the subsequent episodes of erythema migrans was due to a new infection ( presumably transmitted from a new tick bite ) rather than recrudescence of a persistent infection .
moreover , investigators used statistical simulation based on these data to conclude that patients treated for early lyme disease develop protective immunity that is strain specific and that lasts for at least 6 years .
proponents of the existence of  chronic lyme disease  postulate that the organism often persists despite conventional treatment with an antimicrobial , and that very prolonged treatment with antimicrobials is necessary to eliminate the organism . however , other than a few isolated claims ( that could not be replicated ) of recovery of b. burgdorferi from patients with chronic symptoms , there is no evidence that viable organisms persist as a cause of chronic symptoms in humans , despite conventional treatment with antimicrobials [ 48 ] .
in infected patients , b. burgdorferi is present only in low concentrations in blood and spinal fluid and can be difficult to culture .
xenodiagnosis is a method of documenting the presence of a microorganism in tissue by allowing a vector to feed on potentially infected tissue and then examining the vector for the presence of the microorganism it may have ingested . in the past , it was used extensively as a method of diagnosing chagas disease .
some say it may be the most sensitive test in mammals because of evolutionary adaptations of the vector and the microorganism . for example , there is evidence that a chemoattractant is secreted by ixodes scapularis that can enhance migration of b. burgdorferi to its mouthparts while feeding on a host .
the first use of xenodiagnosis to try to detect b. burgdorferi in humans was reported recently . in this study , 36 human subjects ( median age : 55 years ) had 2530 laboratory - raised , pathogen - free , larval i. scapularis ticks placed on them under a retention dressing and were then collected after feeding for 3 to 7 days .
the 36 subjects included 10 patients who had been treated for lyme disease and still had high concentrations of antibodies against the c6 peptide of the membrane protein of b. burgdorferi , 10 patients treated for lyme disease who had post - treatment lyme disease syndrome with continuing symptoms severe enough to impair their normal activities , 5 patients who recently completed antibiotic therapy for erythema migrans , 1 patient early in the course of antibiotic therapy for erythema migrans , and 10 healthy controls .
the patients recently treated for erythema migrans were meant potentially to be positive controls , since it was felt to be unethical to delay treatment of infected patients , but it was thought that these patients had the best chance of testing positive by xenodiagnosis .
biopsies of skin at the site where ticks fed and homogenates of the ticks were cultured for b. burgdorferi .
ticks were also tested for dna of b. burgdorferi by pcr assay and/or by isothermal amplification followed by pcr and electrospray ionization mass spectroscopy .
in addition , attempts were made to infect immunodeficient mice by having the potentially infected ticks feed on them or by inoculation of homogenates of fed ticks into the mice .
no viable organisms were recovered from ticks or from skin biopsies from any of the patients .
one was the patient who was early on in the course of antibiotic treatment for erythema migrans .
most would say that only a positive culture ( recovery of viable organisms ) would constitute positive xenodiagnosis .
it is well recognized that fragments of dna of b. burgdorferi can persist for a very long period after successful antibiotic treatment and killing of viable organisms .
a positive result of a highly sensitive pcr assay of a tick from a patient with post - treatment lyme disease syndrome certainly is provocative
. can persistence of antigens of organisms killed after antimicrobial treatment lead to prolonged non - specific symptoms ?
perhaps persistent ( non - viable ) fragments of organisms can persist and provoke inflammation that leads to symptoms .
certainly the results of this study do not provide evidence for the suppositions that viable b. burgdorferi persist after conventional antimicrobial treatment , or suggest that additional clinical trials of prolonged antimicrobial treatment of patients with only non - specific symptoms after lyme disease are warranted .
it is well recognized that subsequent episodes of erythema migrans are not unusual in patients with an initial episode who receive prompt antimicrobial treatment .
if b. burgdorferi persist despite antimicrobial treatment , it would make sense that subsequent episodes of erythema migrans might be due to the originally infecting strain of the organism . in a study to assess whether such episodes were new infections or recurrences , strains of b. burgdorferi from 17 patients with two or more episodes of culture - proved erythema migrans had 22 paired episodes with organisms available for analysis ( first and second episodes , and , for those with additional episodes , third and fourth episodes ) .
strains were compared by amplifying their dna with polymerase chain reaction ( pcr ) assay of each strain , followed by genotyping of outer surface protein c of each strain . in all instances ,
the strains in each of the paired episodes were different , indicating that each of the subsequent episodes of erythema migrans was due to a new infection ( presumably transmitted from a new tick bite ) rather than recrudescence of a persistent infection .
moreover , investigators used statistical simulation based on these data to conclude that patients treated for early lyme disease develop protective immunity that is strain specific and that lasts for at least 6 years .
proponents of the existence of  chronic lyme disease  postulate that the organism often persists despite conventional treatment with an antimicrobial , and that very prolonged treatment with antimicrobials is necessary to eliminate the organism .
however , other than a few isolated claims ( that could not be replicated ) of recovery of b. burgdorferi from patients with chronic symptoms , there is no evidence that viable organisms persist as a cause of chronic symptoms in humans , despite conventional treatment with antimicrobials [ 48 ] . in infected patients ,
b. burgdorferi is present only in low concentrations in blood and spinal fluid and can be difficult to culture .
xenodiagnosis is a method of documenting the presence of a microorganism in tissue by allowing a vector to feed on potentially infected tissue and then examining the vector for the presence of the microorganism it may have ingested . in the past , it was used extensively as a method of diagnosing chagas disease .
some say it may be the most sensitive test in mammals because of evolutionary adaptations of the vector and the microorganism . for example , there is evidence that a chemoattractant is secreted by ixodes scapularis that can enhance migration of b. burgdorferi to its mouthparts while feeding on a host .
the first use of xenodiagnosis to try to detect b. burgdorferi in humans was reported recently . in this study , 36 human subjects ( median age : 55 years ) had 2530 laboratory - raised , pathogen - free , larval i. scapularis ticks placed on them under a retention dressing and were then collected after feeding for 3 to 7 days .
the 36 subjects included 10 patients who had been treated for lyme disease and still had high concentrations of antibodies against the c6 peptide of the membrane protein of b. burgdorferi , 10 patients treated for lyme disease who had post - treatment lyme disease syndrome with continuing symptoms severe enough to impair their normal activities , 5 patients who recently completed antibiotic therapy for erythema migrans , 1 patient early in the course of antibiotic therapy for erythema migrans , and 10 healthy controls .
the patients recently treated for erythema migrans were meant potentially to be positive controls , since it was felt to be unethical to delay treatment of infected patients , but it was thought that these patients had the best chance of testing positive by xenodiagnosis .
biopsies of skin at the site where ticks fed and homogenates of the ticks were cultured for b. burgdorferi .
ticks were also tested for dna of b. burgdorferi by pcr assay and/or by isothermal amplification followed by pcr and electrospray ionization mass spectroscopy .
in addition , attempts were made to infect immunodeficient mice by having the potentially infected ticks feed on them or by inoculation of homogenates of fed ticks into the mice .
no viable organisms were recovered from ticks or from skin biopsies from any of the patients .
one was the patient who was early on in the course of antibiotic treatment for erythema migrans .
most would say that only a positive culture ( recovery of viable organisms ) would constitute positive xenodiagnosis .
it is well recognized that fragments of dna of b. burgdorferi can persist for a very long period after successful antibiotic treatment and killing of viable organisms .
a positive result of a highly sensitive pcr assay of a tick from a patient with post - treatment lyme disease syndrome certainly is provocative .
can persistence of antigens of organisms killed after antimicrobial treatment lead to prolonged non - specific symptoms ?
perhaps persistent ( non - viable ) fragments of organisms can persist and provoke inflammation that leads to symptoms .
certainly the results of this study do not provide evidence for the suppositions that viable b. burgdorferi persist after conventional antimicrobial treatment , or suggest that additional clinical trials of prolonged antimicrobial treatment of patients with only non - specific symptoms after lyme disease are warranted .
whether or not borrelia burgdorferi can persist after conventional treatment with antimicrobials has been a very controversial issue . 
 two recent studies took different approaches to try to answer this question . 
 in one , investigators showed that , in each of 22 instances in 17 patients with two consecutive episodes of culture - proved erythema migrans , the strains of b. burgdorferi were different based on their genotypes . 
 this indicated that the repeat episodes were due to new infections rather than recrudescence of the original infection . in another study , in which persistence of b. burgdorferi was assessed by using xenodiagnosis 
 , no viable b. burgdorferi were cultured from ticks fed on any of the patients . 
 there continues to be no evidence that viable b. burgdorferi persist in humans after conventional treatment with antimicrobials .