You will be shown an abstract from a biomedical research paper. Given this abstract, your task is to extract all unique entities of the following types: ["Protein", "Chemical"].

Please return the output as a JSON object of the format: {"Chemical" : ["Dexamethasone", ...], "Protein" : ["BRCA-1", ...]}. The keys should be entity types and values should be lists of extracted entities belonging to the corresponding type. If you cannot find entities belonging to a specific type, the value should be [].

Only output the JSON object and do not include any additional text.

Abstract:

X-box binding protein 1 is essential for insulin regulation of pancreatic alpha cell function.
Type 2 diabetes mellitus (T2D) patients often exhibit hyperglucagonemia despite hyperglycemia suggesting defective α-cell function. While endoplasmic reticulum (ER) stress has been suggested to underlie β-cell dysfunction in T2D, its role in α-cell biology remains unclear. X-box binding protein 1 (XBP1) is a transcription factor that plays a crucial role in the unfolded protein response (UPR) and its deficiency in β-cells has been reported to impair insulin secretion leading to glucose intolerance. To evaluate the role of XBP1 in α-cells, we created complementary in vivo (α-cell specific XBP1 knockout (αXBPKO) mice) and in vitro (stable XBP1 knock down α-cell line, αXBPKD) models. αXBPKO mice exhibited glucose intolerance, mild insulin resistance and an inability to suppress glucagon secretion following glucose stimulation. αXBPKD cells exhibited activation of IRE1, an upstream activator of XBP1, leading to phosphorylation of JNK. Interestingly, insulin treatment of αXBPKD cells reduced tyrosine phosphorylation of IRS-1 (pY(896)), and phosphorylation of Akt, while enhancing serine phosphorylation (pS(307)) of IRS-1. Consequently the αXBPKD cells exhibited blunted suppression of glucagon secretion following insulin treatment in the presence of high glucose. Together, these data indicate that XBP1 deficiency in pancreatic α-cells induces altered insulin signaling and dysfunctional glucagon secretion.
{"Chemical": ["tyrosine", "glucose", "serine"], "Protein": ["IRE1", "Akt", "XBP", "glucagon", "JNK", "insulin", "IRS-1", "XBP1", "X-box binding protein 1"]}