You will be shown an abstract from a biomedical research paper. Given this abstract, your task is to extract all unique entities of the following types: ["AnatomicalEntity"].

Please return the output as a JSON object of the format: {"AnatomicalEntity": ["tumor cells", ...]}. The keys should be entity types and values should be lists of extracted entities belonging to the corresponding type. If you cannot find entities belonging to a specific type, the value should be [].

Only output the JSON object and do not include any additional text.

Abstract:

Impaired CK1 delta activity attenuates SV40-induced cellular transformation in vitro and mouse mammary carcinogenesis in vivo. 
Simian virus 40 (SV40) is a powerful tool to study cellular transformation in vitro, as well as tumor development and progression in vivo. Various cellular kinases, among them members of the CK1 family, play an important role in modulating the transforming activity of SV40, including the transforming activity of T-Ag, the major transforming protein of SV40, itself. Here we characterized the effects of mutant CK1delta variants with impaired kinase activity on SV40-induced cell transformation in vitro, and on SV40-induced mammary carcinogenesis in vivo in a transgenic/bi-transgenic mouse model. CK1delta mutants exhibited a reduced kinase activity compared to wtCK1delta in in vitro kinase assays. Molecular modeling studies suggested that mutation N172D, located within the substrate binding region, is mainly responsible for impaired mutCK1delta activity. When stably over-expressed in maximal transformed SV-52 cells, CK1delta mutants induced reversion to a minimal transformed phenotype by dominant-negative interference with endogenous wtCK1delta. To characterize the effects of CK1delta on SV40-induced mammary carcinogenesis, we generated transgenic mice expressing mutant CK1delta under the control of the whey acidic protein (WAP) gene promoter, and crossed them with SV40 transgenic WAP-T-antigen (WAP-T) mice. Both WAP-T mice as well as WAP-mutCK1delta/WAP-T bi-transgenic mice developed breast cancer. However, tumor incidence was lower and life span was significantly longer in WAP-mutCK1delta/WAP-T bi-transgenic animals. The reduced CK1delta activity did not affect early lesion formation during tumorigenesis, suggesting that impaired CK1delta activity reduces the probability for outgrowth of in situ carcinomas to invasive carcinomas. The different tumorigenic potential of SV40 in WAP-T and WAP-mutCK1delta/WAP-T tumors was also reflected by a significantly different expression of various genes known to be involved in tumor progression, specifically of those involved in wnt-signaling and DNA repair. Our data show that inactivating mutations in CK1delta impair SV40-induced cellular transformation in vitro and mouse mammary carcinogenesis in vivo.
{"AnatomicalEntity": ["lesion", "tumors", "in situ carcinomas", "cell", "tumor", "breast cancer", "invasive carcinomas", "SV-52 cells", "cellular", "mammary"]}