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thirty - two eyes of 16 patients who underwent unilateral ptosis surgery were enrolled in the study .
the patients with other ocular disease , dry eye , history of a systemic disease that would affect ocular surface , history of contact lens use , and prior conjunctival surgery were excluded from the study . all patients underwent ptosis repair by an mcr with a technique similar to the described by putterman .
a subjective dry eye questionnaire was given to the patients questioning various dry eye symptoms , frequency of dryness , grittiness , burning , redness , crust formation on lashes and stickiness of eyelids in the morning .
responses were graded as follows : 0 - never ; 1 - rare ; 2 - sometimes ; 3 - frequently ; or 4 - always .
schirmer and tbut tests were performed after an application of a drop of proparacaine hydrochloride 0.5% .
fluorescein staining was recorded for 5 regions of the cornea ( superior , inferior , nasal , temporal , central ) and 4 regions of the conjunctiva ( superior , inferior , nasal , temporal ) .
the total staining score was the sum of the scores of all regions . rose - bengal staining ( rbs ) of the conjunctiva was performed by using a contacare rose - bengal ophthalmic strip ( contacare , baroda , india ) ; initially wetted with non - preserved buffered saline and then instilled on the inferior bulbar conjunctiva .
the eyelid margin was checked for meibomian gland disease ( mgd ) with slit lamp examination .
grade-2 indicated 3 to 4 blocked glands with thick secretions but without expression of glands .
impression cytology specimens were collected from the temporal and nasal conjunctiva of each eye using nitrocellulose ( sartorius , gttingen , germany ) filter paper .
the quantitative studies of conjunctival goblet cells and squamous metaplasia of conjunctival epithelial cells were conducted by taking photographs with a calibrated grid under a light microscope .
five non - overlapping areas of each randomly - selected sample were photographed and averaged for a single score .
the specimens were also assigned a grade of conjunctival epithelial squamous metaplasia according to nelson 's grading scheme .
the results were analyzed by using paired t - test and wilcoxon matched pair test .
patients were followed - up for mean 18.5  10.2 ( 4 - 40 ) months .
the mean subjective dry eye questionnaire score was 2.68  3.98 points in operated eyes and 2.00  2.63 points in control eyes .
there was no difference in the st and tbut scores before and after surgery in both operated and in the control eyes ( p = 0.79 , p = 1.00 , respectively ) .
the mean fluorescein and rbs scores , meibomian gland disease score and squamous metaplasia grading score of operated and non - operated eyes of each patient also showed no significant differences [ table 1 ] .
tear function and ocular surface examinations an impression cytology analysis showed no statistically significant change in goblet cell densities between operated and the control eyes ( > 0.05 ) .
conjunctival epithelium contains goblet cells that secrete mucin and the glands of krause and wolfring , which are responsible for basal tear secretion .
any damage in tear film leads to epithelial defects in the cornea , and these defects are stained with fluorescein .
this stain is blocked by tear proteins such as albumin and mucin , which cover the ocular surface . decreased mucin production plays a major role in ocular surface staining with rose - bengal . in comparison of operated and control eyes in terms of fluorescein and rbs , no significant difference was found between the eyes .
the pathological examination of samples taken from upper and lower bulbar conjunctiva of patients revealed no statistically significant difference in goblet cell density and grade of conjunctival metaplasia between operated and control eyes .
conjunctival forniceal shortening due to conjunctival resection has more impact on dry eye states with conjunctival deficiency such as , anophthalmic socket and conjunctival cicatrizing diseases .
the suture used in mcr may abrade into the filtration area and may be problematic in patients with trabeculectomy .
some investigators believe that an excision of conjunctiva may damage the accessory lacrimal glands of wolfring , located in the upper tarsal border , which may aggravate dry eye states .
we have incorporated all the frequently applied clinical and laboratory tests to evaluate the effects of mcr surgery on tear film stability .
our results conclude that an mcr surgery does not have any adverse effects tear film production and stability .
further studies with a larger patient population might shed light on the effect of an mcr on tear film and ocular surface .
muller muscle - conjunctival resection ( mcr ) is a surgical technique to correct mild and moderate ptosis . in this study , 
 tear function tests and ocular surface are evaluated in patients who underwent unilateral surgery . 
 sixteen patients with normal preoperative tear function who underwent unilateral mcr were evaluated prospectively . 
 the fellow eyes of the patients were taken as the control group . 
 a dry eye assessment questionnaire , schirmer testing , tear film break - up time , fluorescein stain , rose - bengal stain , and conjunctival impression cytology were used to assess the tear film functions and ocular surface changes in the operated and non - operated eyes . 
 there was no statistically significant difference in the tear function tests and goblet cell densities between the operated and non - operated eyes . 
 the results indicate that an mcr procedure has no apparent effect on tear function tests and goblet cell density in patients with normal preoperative tear function .