You are provided with the abstract of a scientific paper and the abstracts of the papers it references. Your task is to write a Related Work section for the main paper. This section should concisely summarize and integrate the key points from the cited abstracts, reflecting how they relate to the main paper's contributions and context. In your summary, use numerical citation markers like [1], [2], etc., to refer to the corresponding papers.

Main Abstract:
Recently several deep learning models have been used for DNA sequence based classification tasks. Often such tasks require long and variable length DNA sequences in the input. In this work, we use a sequence-to-sequence autoencoder model to learn a latent representation of a fixed dimension for long and variable length DNA sequences in an unsupervised manner. We evaluate both quantitatively and qualitatively the learned latent representation for a supervised task of splice site classification. The quantitative evaluation is done under two different settings. Our experiments show that these representations can be used as features or priors in closely related tasks such as splice site classification. Further, in our qualitative analysis, we use a model attribution technique Integrated Gradients to infer significant sequence signatures influencing the classification accuracy. We show the identified splice signatures resemble well with the existing knowledge.

Cited Abstract(s):
[1]: 

[2]: This paper applies a deep convolutional highway MLP framework to classify genomic sequences on the transcription factor binding site task. To make the model understandable, we propose an optimization driven strategy to extract "motifs", or symbolic patterns which visualize the positive class learned by the network. We show that our system, Deep Motif (DeMo), extracts motifs that are similar to, and in some cases outperform the current well known motifs. In addition, we find that a deeper model consisting of multiple convolutional and highway layers can outperform a single convolutional and fully connected layer in the previous state-of-the-art.

[3]: Motivation: During the last decade, improvements in high-throughput sequencing have generated a wealth of genomic data. Functionally interpreting these sequences and finding the biological signals that are hallmarks of gene function and regulation is currently mostly done using automated genome annotation platforms, which mainly rely on integrated machine learning frameworks to identify different functional sites of interest, including splice sites. Splicing is an essential step in the gene regulation process, and the correct identification of splice sites is a major cornerstone in a genome annotation system. Results: In this paper, we present SpliceRover, a predictive deep learning approach that outperforms the state-of-the-art in splice site prediction. SpliceRover uses convolutional neural networks (CNNs), which have been shown to obtain cutting edge performance on a wide variety of prediction tasks. We adapted this approach to deal with genomic sequence inputs, and show it consistently outperforms already existing approaches, with relative improvements in prediction effectiveness of up to 80.9 when measured in terms of false discovery rate. However, a major criticism of CNNs concerns their 'black box' nature, as mechanisms to obtain insight into their reasoning processes are limited. To facilitate interpretability of the SpliceRover models, we introduce an approach to visualize the biologically relevant information learnt. We show that our visualization approach is able to recover features known to be important for splice site prediction (binding motifs around the splice site, presence of polypyrimidine tracts and branch points), as well as reveal new features (e.g. several types of exclusion patterns near splice sites).

[4]: The binding specificities of RNA- and DNA-binding proteins are determined from experimental data using a ‘deep learning’ approach.

[5]: Motivation: Regulatory sequences are not solely defined by their nucleic acid sequence but also by their relative distances to genomic landmarks such as transcription start site, exon boundaries or polyadenylation site. Deep learning has become the approach of choice for modeling regulatory sequences because of its strength to learn complex sequence features. However, modeling relative distances to genomic landmarks in deep neural networks has not been addressed. Results: Here we developed spline transformation, a neural network module based on splines to flexibly and robustly model distances. Modeling distances to various genomic landmarks with spline transformations significantly increased state-of-the-art prediction accuracy of in vivo RNAb-inding protein binding sites for 120 out of 123 proteins. We also developed a deep neural network for human splice branchpoint based on spline transformations that outperformed the current best, already distance-based, machine learning model. Compared to piecewise linear transformation, as obtained by composition of rectified linear units, spline transformation yields higher prediction accuracy as well as faster and more robust training. As spline transformation can be applied to further quantities beyond distances, such as methylation or conservation, we foresee it as a versatile component in the genomics deep learning toolbox.

[6]: Splicing refers to the elimination of noncoding regions in transcribed pre-messenger ribonucleic acid (RNA). Discovering splice sites is an important machine learning task that helps us not only to identify the basic units of genetic heredity but also to understand how different proteins are produced. Existing methods for splicing prediction have produced promising results, but often show limited robustness and accuracy. In this paper, we propose a deep belief network-basedmethodology for computational splice junction prediction. Our proposal includes a novel method for training restricted Boltzmann machines for class-imbalanced prediction. The proposed method addresses the limitations of conventional contrastive divergence and provides regularization for datasets that have categorical features. We tested our approach using public human genome datasets and obtained significantly improved accuracy and reduced runtime compared to state-of-the-art alternatives. The proposed approach was less sensitive to the length of input sequences and more robust for handling false splicing signals. Furthermore,we could discover noncanonical splicing patterns that were otherwise difficult to recognize using conventional methods. Given the efficiency and robustness of our methodology, we anticipate that it can be extended to the discovery of primary structural patterns of other subtle genomic elements.

[7]: Abstract Identification of intron boundaries, called splice junctions, is an important part of delineating gene structure and functions. This also provides valuable insights into the role of alternative splicing in increasing functional diversity of genes. Identification of splice junctions through RNA-seq is by mapping short reads to the reference genome which is prone to errors due to random sequence matches. This encourages identification of splicing junctions through computational methods based on machine learning. Existing models are dependent on feature extraction and selection for capturing splicing signals lying in the vicinity of splice junctions. But such manually extracted features are not exhaustive. We introduce distributed feature representation, SpliceVec, to avoid explicit and biased feature extraction generally adopted for such tasks. SpliceVec is based on two widely used distributed representation models in natural language processing. Learned feature representation in form of SpliceVec is fed to multilayer perceptron for splice junction classification task. An intrinsic evaluation of SpliceVec indicates that it is able to group true and false sites distinctly. Our study on optimal context to be considered for feature extraction indicates inclusion of entire intronic sequence to be better than flanking upstream and downstream region around splice junctions. Further, SpliceVec is invariant to canonical and non-canonical splice junction detection. The proposed model is consistent in its performance even with reduced dataset and class-imbalanced dataset. SpliceVec is computationally efficient and can be trained with user-defined data as well.

[8]: 

[9]:
Most of the previous work for feature learning and motif identification has been in the context of supervised learning. [7] proposed a method for learning distributed feature representation of splice junctions using k-mers. Spline transformation was introduced by [5] to improve upon traditional neural networks to learn better representations and improve prediction accuracy. [9] used a memory matching network to dynamically learn a memory bank of motifs using sequence classification task. [6] proposed a Restricted Boltzmann Machine based model with a new training method called boosted Contrastive Divergence to predict non-canonical splice sites and learn non-canonical feature vectors that couldn't be identified by traditional methods. Some works have also used Convolutional Neural Networks(CNN) in order to learn representations implicitly [3] [4] [1] [2] [8] .