You will be shown an abstract from a biomedical research paper. Given this abstract, your task is to extract all unique entities of the following types: ["RNA", "CellLine", "Protein", "DNA", "CellType"].

Please return the output as a JSON object of the format: {"DNA" : ["TAL1", ...], "RNA": ["mRNAs", ...], "CellLine": ["T cells", ...], "CellType": ["monocytes", ...], "Protein": ["IL-2", ...]}. The keys should be entity types and values should be lists of extracted entities belonging to the corresponding type. If you cannot find entities belonging to a specific type, the value should be [].

Only output the JSON object and do not include any additional text.

Abstract:

Kappa B binding proteins are constitutively expressed in an IL-2 autocrine human T cell line. The IL-2 and the IL-2-R alpha genes are both expressed transiently in normal T lymphocytes after Ag or mitogen activation. In contrast, the human T cell line, IARC 301, expresses these two genes constitutively and we have previously demonstrated that its growth depends on the autocrine production of this T cell growth factor and high affinity IL-2R. To dissect the molecular basis for the unusual persistent expression of the IL-2 and IL-2-R alpha genes in these IARC 301 T cells, we have analyzed the interactions of constitutively expressed nuclear proteins with the 5' flanking regions of the IL-2 and IL-2-R alpha genes using both DNase I footprinting and gel retardation techniques. We have found that a region in both genes (-276 to-250 for IL-2-R alpha and-203 to-183 for IL-2), which corresponds to a kappa B enhancer element, is specifically protected by nuclear proteins from IARC 301. In agreement with this finding, both the IL-2 and IL-2-R alpha promoters are active in transient transfection assays in IARC 301 cells. In contrast, mutation of the kappa B enhancer results in markedly attenuated activities of both promoters. Two proteins binding the kappa B sequence, NF-kappa B and KBF1, are constitutively expressed in IARC 301 nuclei and induced by PMA and PHA in Jurkat. They bind to the kappa B motifs with different relative affinities that may reflect their different contribution in the expression of various promoters.
{"CellLine": ["Jurkat", "IARC 301", "IL - 2 autocrine human T cell line", "IARC 301 cells", "human T cell line"], "CellType": ["normal T lymphocytes", "T cells"], "DNA": ["kappa B sequence", "IL - 2 and IL - 2 - R alpha genes", "5\u0027 flanking regions", "IL - 2 - R alpha", "IL - 2 and IL - 2 - R alpha promoters", "kappa B enhancer element ,", "promoters", "kappa B motifs", "IL - 2", "IL - 2 and the IL - 2 - R alpha genes"], "Protein": ["PHA", "DNase I", "T cell growth factor", "constitutively expressed nuclear proteins", "nuclear proteins", "kappa B enhancer", "Kappa B binding proteins", "high affinity IL - 2R", "NF - kappa B", "KBF1"], "RNA": []}