Safety and Efficacy Study of Regulatory T Cells in Treating Autoimmune Hepatitis
Safety and Efficacy Study of Regulatory T Cells in Treating Autoimmune Hepatitis
Separated and expanded the CD4+CD25+CD127- Tregs from peripheral blood of autoimmune
 hepatitis patients and administrate the cells (5 x 106 cells/kg) into patients.
The trial will be carried out in autoimmune patients. The investigators will isolate
 CD4(cluster of differentiation)+CD25(cluster of differentiation25)+CD127(cluster of
 differentiation127)- Tregs from these patients, and expand them with IL(interleukin)-2
 retinoid acid and anti-CD3(cluster of differentiation 3) /CD28(cluster of differentiation 28)
 beads. The patients will be subsequently treated with single infusion of CD4+CD25+CD127-
 Tregs (10-20 x 106 cells/kg).

 In the clinical trials, the investigators will monitor the number of Tregs in patients at
 different periods, and to test their suppressive functions in vitro. Also the function and
 biopsy of liver will be processed to determine the efficacy of Treg therapy.
Inclusion Criteria:

 1. Written informed consent

 2. Autoimmune hepatitis(according to the criteria defined by the international autoimmune
 hepatitis Group ,Hepatology, 2008;48:169-176)

 3. Negative pregnancy test

 4. Moderately active disease under standard treatment

 Exclusion Criteria:

 1. Hepatocellular carcinoma or other Malignancies

 2. Pregnant or lactating women

 3. Vital organs failure (Cardiac, Renal or Respiratory, et al)

 4. Sepsis

 5. Active thrombosis in the portal or hepatic veins

 6. Concomitant psychiatric disease or any other chronic illness or drug-abuse that could
 interfere with the ability to comply with the protocol or to give informed consent

 7. Surgery during the last 2 months or surgery planned during the study,

 8. Participation in other biomedical research in the last 3 months or planned during the
 study.