NGS-Guided(G) Regimens(R) of Anti-tuberculosis(A) Drugs for the Control(C) and Eradication(E) of MDR-TB
NGS-Guided(G) Regimens(R) of Anti-tuberculosis(A) Drugs for the Control(C) and Eradication(E) of MDR-TB
Tuberculosis (TB) has been one of the top 10 causes of death worldwide from a single
 infectious agent, ranking above HIV/AIDS. Management and eradication of this disease is being
 hindered by the emergence of multidrug-resistant TB (MDR-TB) and extensively drug resistant
 TB (XDR-TB). Globally, there were estimated 10.4 million cases of TB and 490,000 cases of
 MDR-TB in 2016. China accounts for around 8.6% (0.895/10.4 million) of the global TB burden,
 ranking third in the top 3 countries (India, Indonesia, China) with the highest number of TB
 cases and ranking first with the largest number of MDR/ Rifampin-Resistant (RR)-TB cases. The
 treatment success rate for MDR-TB using the 18-24-month conventional World Health
 Organization (WHO) regimen was estimated to be about 54% worldwide and 41% for China in 2016,
 which remains unacceptably low.

 The poor MDR-TB treatment success rates suggest that current drug regimens are suboptimal. In
 addition, they are costly with a high pill burden, as many drugs, with significant potential
 for adverse events, are given for a long duration. These factors also inhibit good treatment
 compliance with further negative impact on treatment outcomes. According to previous studies,
 treatment outcomes of MDR-TB could be affected by drug resistance of pivotal drugs in MDR-TB
 regimen, such as fluoroquinolones, second-line injectable agents and pyrazinamide. The
 available drug-resistance information could help physicians decide the proper regimens for
 MDR-TB patients, which may prevent the useless prescription and evitable adverse.

 Therefore, the individualized regimen based on the resistance profile of the bacteria and
 patients' drug tolerance should be aimed for high-quality treatment for MDR-TB in the future.
 A precision individualized treatment approach based on the rapid molecular drug
 susceptibility tests of second line drugs may assist clinicians in making more suitable
 regimen and improve the treatment outcome of MDR-TB. Also, precision regimen offers the
 opportunity to improve treatment of drug-resistant tuberculosis through reduced toxicity
 while reducing the risk of resistance amplification and further transmission at a population
 level.

 The purpose of this research is to assess the feasibility and effects of individualized
 regimen that is guided by rapid molecular drug susceptibility tests of key second-line drugs
 through next generation sequencing. Meanwhile, the study will evaluate a short course
 regimens of drugs among "simple MDR-TB" patients who are proven to be sensitive to
 fluoroquinolones ,injectable second-line drugs and pyrazinamide.
The GRACE-TB study is a multi-center, open-label, randomized, controlled trial in patients
 with MDR-TB. This study will assess the feasibility and effects of individualized regimen for
 MDR-TB based on rapid molecular drug susceptibility tests（DSTs） of key second-line drugs
 through next generation sequencing (NGS) and try to improve the treatment outcome of MDR-TB.
 And the study will evaluate a shorter course regimen among patients who are proven to be
 sensitive to fluoroquinolones (FQs) or second-line injectable drugs(SLIDs) or pyrazinamide
 (PZA) through NGS.

 A total of 488 participants with MDR-TB will be recruited and followed up until 18 months
 after the end of treatment. During randomization, eligible patients will be assigned in a 1:3
 ratio to one of the following groups: a control group, which is treated with WHO-approved
 MDR-TB regimen, composed of 6 months of PZA, amikacin (Am) ,moxifloxacin (MFX), prothionamide
 (PTO), and Cycloserine (Cs), followed by 18 months of PZA, MFX, PTO and Cs; a NGS-guided
 group, which is treated with one individualized regimen that is guided by the drug
 susceptability test results of FQs/PZA/ SLIDs through NGS.

 About 366 patients will be enrolled in the NGS-guided group. Based on the molecular DST
 results of FQs/PZA/ SLIDs , patients proven to be sensitive to PZA, FQs and SLIDs will be
 divided into to the "simple MDR-TB group" and those with resistant to at least one of
 FQs/PZA/ SLIDs will be divided into to "complicated MDR-TB group".

 Patients in the "simple MDR-TB group" will be assigned randomly in a 1:1 ratio to one of the
 following daily regimen: a 9-month regimen(Regimen A) which consists of 4-month intensive
 therapy of PZA, Am, MFX,PTO, Cs, followed by 5-month consolidation therapy of PZA, MFX, PTO
 and Cs; a 12-month regimen(Regimen B) which consists of 6-month intensive therapy of PZA, Am,
 MFX,PTO, Cs, followed by 6-month consolidation therapy of PZA, MFX, PTO and Cs.

 Patients in the "complicated MDR-TB group" will be treated a regimen (Regimen C) in which the
 resistant drug(s) will be replaced by the other WHO recommended drugs for MDR-TB such as
 linezolid, clofazimine or ethambutol based on the DST results. The duration of treatment in
 the "complicated MDR-TB group" is consistent with control group, with 6 months of intensive
 phase and 18 months of consolidation phase.

 The primary objective is to compare the proportion of patients with a favorable efficacy
 between the NGS-guided group and the control group. The second objective is to assess whether
 the proportion of simple MDR-TB patients with a favorable efficacy outcome of Regimen A is
 not inferior to Regimen B. The participants will be followed up to 18 months after the end of
 the treatment. The data accrued to 18 months after the end of treatment will be used in
 primary and secondary analyses.

 Safety evaluations performed are the routine lab tests, blood glucose, hearing, vital signs,
 electrocardiograph (ECG), reporting of adverse events, physical examinations and chest CT.
 Adverse events will be monitored and promptly managed during the whole treatment course.
Inclusion Criteria:

 -  Patients who are diagnosed with active MDR-TB. MDR-TB is defined as resistance to the
 following two drugs: Isoniazid and Rifampicin.

 -  Patients who are smear positive and sputum culture positive for mycobacterium
 tuberculosis

 -  HIV negative.

 -  The patients should be voluntarily entering the study and willing to sign up the
 consent form after full knowledge of the risks, schedule, drug features of this study.

 Exclusion Criteria:

 -  Known allergy or intolerance to the drugs in this study

 -  Liver damage (Hepatic encephalopathy; ascites; prothrombin time prolonged 2 seconds
 compared with normal controls; blood bilirubin 3 times greater than the upper limit of
 the normal range)

 -  Platelets <150x10^9 / L, WBC < 3x10^9 / L.

 -  Abnormal ECG (Male patients with prolonged QT interval exceeding 430ms,

 -  Female patients with prolonged QT interval exceeding 450ms)

 -  Serum creatinine 1.5 times higher than upper limit

 -  Fasting blood-glucose higher than 8.0 mmol/L

 -  Patients who are on medication that effect the results of the drugs in this study
 Karnofsky score<50% (see appendix)

 -  Women who are pregnant or breastfeeding

 -  HIV positive

 -  Participating in other clinical trials in the past three months

 -  Patients with mental illness and severe neurosis

 -  Patients who have poor compliances

 -  Any special circumstances in which the research physicians believe that is not
 suitable for this study.