Clinical Trial in Subjects With Mild to Moderate Acne Vulgaris
Clinical Trial in Subjects With Mild to Moderate Acne Vulgaris
The purpose of this study is to demonstrate the safety, tolerability and efficacy of B244
 administered over 12 weeks to participants with mild to moderate acne vulgaris relative to
 placebo.
This is a Phase IIb/III, randomized, double blinded, decentralized clinical trial evaluating
 the safety, tolerability, and efficacy of B244 compared to placebo in the treatment of acne
 vulgaris

 1.1. Primary Objectives

 1. To evaluate the safety and tolerability of B244 in participants with acne vulgaris

 2. To assess the efficacy of B244 in participants with mild to moderate acne vulgaris from
 baseline to week 12 (end of treatment) by:

 i) Reduction in inflammatory and non-inflammatory lesion count ii) IGA success

 3. Improvement in patient reported quality of life score using the Skindex-16 questionnaire
 in participants with acne vulgaris from baseline to week 12

 1.2. Secondary Objectives 1. To evaluate the efficacy of B244 in participants with mild to
 moderate acne vulgaris from baseline to weeks 2, 4, 8, and 16: i) Reduction in inflammatory
 and non-inflammatory lesion count ii) IGA success iii) Improvement in patient reported
 quality of life score using the Skindex-16 questionnaire

 1.3. Exploratory Objective

 1. To evaluate facial skin microbiota in participants with acne vulgaris at baseline, week 4,
 week 8, week 12, and week 16 in B244-treated participants compared to placebo from: i) Skin
 swabs will be taken from forehead, nose, both cheeks and chin All participants (placebo and
 B244) will undergo skin swabs and testing.
Inclusion Criteria:

 Participants eligible for enrollment in the study must meet all the following criteria:

 1. Male and females age 18 or older

 2. Clinical diagnosis of mild to moderate facial acne vulgaris defined as:

 1. ≥5 inflammatory lesions, and;

 2. ≥10 non-inflammatory lesions, and;

 3. IGA 2-3

 3. Willing to refrain from using any treatments, other than the investigational product,
 for acne present on the face. This includes the use of antibiotics for the treatment
 of acne. Topical acne treatments that do not have significant or measurable systemic
 absorption (e.g., benzoyl peroxide, salicylic acid) are allowed for treatment of
 non-facial acne.

 4. Willing and able to provide informed consent and to comply with the study protocol.

 Exclusion Criteria:

 1. Pregnant and/or lactating females

 2. Continuous or planned use of tanning booths or excessive sun exposure, in the opinion
 of the Investigator.

 3. Active cystic acne or acne conglobata, acne fulminans, and secondary acne

 4. Two or more active nodular lesions

 5. Treatment with over-the-counter topical medications for the treatment of acne vulgaris
 including benzoyl peroxide, topical anti-inflammatory medications, corticosteroids,
 α-hydroxy/glycolic acid, or topical probiotics including commercially available
 product AO+Mist on the face within 7 days prior to baseline.

 6. Treatment with systemic corticosteroids within 28 days prior to baseline.

 7. Treatment with systemic antibiotics or systemic anti-acne drugs within 7 days prior to
 baseline.

 8. Prescription topical retinoid use on the face within 7 days of baseline (e.g.,
 tretinoin, tazarotene, adapalene).

 9. Commencement of new hormonal therapy or dose change to hormonal therapy within 90 days
 prior to baseline. Dose and frequency of use of any hormonal therapy started more than
 90 days prior to baseline must remain unchanged throughout the study. Hormonal
 therapies include, but are not limited to, estrogenic and progestational agents such
 as birth control pills.

 10. Use of androgen receptor blockers (such as spironolactone or flutamide) in the 7 days
 prior to randomization.

 11. Oral retinoid use (e.g., isotretinoin) within 180 days prior to baseline or vitamin A
 supplements greater than 10,000 units/day within 180 days of baseline.

 12. Cosmetic facial procedures (chemical or laser peel, microdermabrasion, etc.) within
 the 28 days of the first dose or during the study.

 13. History of sensitivity to any of the study medications, or components thereof or a
 history of drug or other allergy that, in the opinion of the Investigator or Medical
 Monitor, contraindicates their participation.

 14. Any condition that the study Investigator feels would indicate that participation
 would not be in the best interest of the participant.

 15. The participant has been previously randomized in this study.

 16. The participant has received an investigational product within 30 days or 5
 half-lives, whichever is longer prior to randomization.