Postoperative Pain and Antibacterial Efficacy After Root Canal Irrigation With Curcumin or Sodium Hypochlorite
Postoperative Pain and Antibacterial Efficacy After Root Canal Irrigation With Curcumin or Sodium Hypochlorite
This study aims to compare the effect of 2% curcumin solution versus 2.5% sodium hypochlorite
 as irrigating solutions in necrotic mandibular molars in terms of postoperative pain and
 antibacterial effect.
patients with necrotic mandibular molars will be selected

 1. Diagnostic procedures:

 -  Personal information (name, age, gender, address and phone number), full medical
 and dental histories using schematic charts will be obtained from all patients
 participating in this research.

 -  History of chief complaint will be obtained in the dental history chart.

 -  Clinical and radiographic evaluation for each tooth included in this study will be
 recorded. The tooth will be examined for presence of extensive caries or pulp
 exposures. Soft tissue examination and tooth mobility evaluation will be done by
 percussion and palpation.

 -  Extraoral examination will be performed by visual inspection of the face and neck
 and palpation for any swollen lymph nodes.

 -  Each tooth will be evaluated for sensitivity using electric pulp tester. Diagnosis
 will also be confirmed by the absence of bleeding after access cavity preparation.

 -  Final Diagnosis: Mature mandibular asymptomatic necrotic molars with normal
 periapical radiographic appearance.

 -  The operator will explicitly explain the trial steps to the patients, and an
 informed consent will be signed by patients who accept enrollment.

 -  Pain scale chart will be given to each patient to rate his /her pain level before
 endodontic treatment as preoperative reading using a Numerical Rating Scale (NRS).

 2. Endodontic procedures:

 -  All the performed steps of root canal treatment will be included in the chart of
 endodontic procedures.

 -  The tooth will be isolated with rubber dam to maintain aseptic field, after
 isolation the tooth and surrounding field will be disinfected by a protocol using
 3% hydrogen peroxide and 2.5% sodium hypochlorite before and after coronal access
 cavity preparation.

 -  The initial sample S1 will be collected from root canals before preparation using
 sterile paper points. Each root canal will be dried with 3 sterile paper points
 ISO# 15 which will be left inside the root canal for 1 minute each with pumping
 movements to generate a suspension with bacteria of the main pulpal area.

 -  The paper points will be immediately placed in sterile test tubes containing
 reduced transport medium of thioglycolate and sent to the Microbiology Laboratory,
 Faculty of Medicine, Cairo University for microbiologic processing to be cultured
 within one hour.

 -  Root canals will be mechanically prepared in a crown-down approach using ProTaper
 Universal NiTi files in an X-Smart endodontic motor according to the manufacture
 instructions.

 -  The canals will be thoroughly irrigated between every two subsequent files using 2
 ml of 2.5% NaOCl using a side vented needle (gauge 29) to control the possibility
 of irrigant apical extrusion.

 -  After complete root canal preparation all canals will be flushed with 17 % EDTA
 followed by sterile saline.

 3. Random allocation:

 -  According to the randomization sequence, after complete root canal preparation
 patients will be assigned to one of 2 groups:

 -  Intervention Group (G1): each canal will be flushed using 5 ml of curcumin
 solution in a plastic syringe with a side vented needle (gauge 29) inserted 1
 ml shorter than the working length.

 -  Comparator Group (G2): each canal will be flushed using 5 ml of 2.5% sodium
 hypochlorite in a plastic syringe with side vented needle (gauge 29) inserted
 1 ml shorter than the working length.

 -  After flushing the canals with each irrigating solution all canals will be flushed
 with 10 ml sterile saline and the final sample S2 will be collected in the same
 manner as S1 using sterile paper points ISO # 30/0.02 or # 40/0.02, corresponding
 to the size of the master apical file F3 or F4, respectively.

 -  Root canals will be dried using ProTaper paper points. The root canals will be
 obturated using the modified single cone technique with resin based root canal
 sealer and ProTaper gutta-percha cones size F3 or F4 according to the size of the
 final finishing file, together with auxiliary cones ISO # 25/0.02.

 -  After obturation, a cotton pellet will be placed in the pulp chamber and the access
 cavity will be sealed with a temporary filling.

 -  All patients will be asked to record their postoperative pain level on Numerical
 Rating Scale (NRS) at 8, 24 and 48 hours postoperatively.
Inclusion Criteria:

 1. Patient's age between 18-50 years.

 2. Male and female.

 3. Systemically healthy patients (ASA I or II).

 4. Mature mandibular molar teeth with: Necrotic pulps, normal periapical radiographic
 appearance and no intra-canal calcifications or internal resorptive lesions

 Exclusion Criteria:

 1. Medically compromised patients having substantive functional limitations (ASA III or
 IV).

 2. Patients having two or more adjacent teeth requiring endodontic treatment.

 3. Patients who received antibiotic therapy within the last three months before
 treatment.

 4. Patients administered analgesics in the last 24 hours before treatment.

 5. Teeth with:

 -  Vital pulps.

 -  Immature apices.

 -  Acute periapical abscess, swellings and facial cellulitis.

 -  Periodontally hopeless (mobility grade II or III).

 -  Previous root canal treatment.

 -  Non-restorable coronal portion.

 6. TMJ problems, bruxism or traumatic occlusion.

 7. Inability to perceive the given instructions