Preoperative Glucocorticoid Use in Major Hepatectomy
Preoperative Glucocorticoid Use in Major Hepatectomy
Background: Reducing postoperative complications remains a dominant challenge for all
 clinicians. By minimizing the incidence of adverse outcomes, health care costs and patient
 recovery can be improved. A number of studies have documented that fewer postoperative
 adverse events occur with the preoperative use of glucocorticoids (GC). Two small manuscripts
 support the use of GC with particular reference to liver resection.

 Major hepatectomy can be associated with numerous adverse outcomes (hemorrhage, bile leak,
 liver failure, wound infection other infectious complications). This rate approaches 54% in
 some trials. Additionally, the liver is responsible for numerous metabolic functions and
 actively participates in the acute phase response via the generation of inflammatory
 mediators and cytokines. Glucocorticoids play an important role in suppressing the
 over-synthesis of pro-inflammatory cytokines and therefore may assist in reducing
 postoperative complications. These pro-inflammatory cytokines recruit leukocytes at the site
 of injury, leading to hepatic parenchymal cell damage. Decreasing pro-inflammatory cytokine
 ratio may therefore reduce hepatic damage.

 Methods: The investigators will complete a randomized controlled trial evaluating the
 incidence of postoperative complications and overall hospital length of stay in adult
 patients following preoperative vs. no administration of 500 mg of methylprednisolone (MP)
 within 30 minutes of starting a hepatectomy. This trial will employ block randomization and
 intention to treat protocols. Four HPB surgeons at University of Calgary will participate in
 the trial with a planned inclusion of all patients scheduled to undergo hepatectomy in a
 period of 2 years.

 Analysis: Standard statistical analysis will include normally or near-normally distributed
 variables reported as means and non-normally distributed variables as medians. Means will be
 compared using the student's t test and medians using the Mann-Whitney U test. Differences in
 proportions among categorical data will be assessed using Fischer's exact test. A p value <
 0.05 will represent statistical significance for all comparisons.

 Hypothesis: Preoperative GC administration will decrease the incidence of postoperative
 complications and overall hospital length of stay following hepatectomy.

 Potential Impact: Health care utilization and economics are an expanding area of importance.
 Reducing post-hepatectomy complications are crucial to this end.
Introduction:

 Major hepatectomy lead to significant postoperative anatomical and physiological changes. The
 liver dictates the systemic metabolic environment and helps maintain homeostasis. When
 segments of liver are removed, it is challenged to regenerate and restore cellular volume, as
 well as continuing to perform mandated metabolic functions. Major hepatectomy can result in
 multiple complications (hemorrhage, biliary fistulae, wound infection, liver failure) with an
 overall complication rate up to 54% in some studies.

 Recent studies purport that preoperative administration of GC may suppress the acute phase
 response during the early postoperative period. GCs are steroid hormones with a variety of
 immunological and biochemical functions (anti-inflammatory attenuation of the acute phase
 response by binding to intra-cytoplasmic GC receptors and therefore increased transcription
 of anti-inflammatory proteins interleukin-10 (IL-10), IL-1-receptor antagonist (IL-Ra),
 lipocortin-1 and neutral endopeptidase, or direct inhibition of activated transcription
 factors (nuclear factor kB, activator protein-1)). GC can also suppress gene expression by
 altering the chromatin structure and tightening DNA coils.

 The acute phase response is a neuro-humoral and time-limited defensive mechanism, which aims
 to restore homeostasis. It is characterized by fever, leukocytosis, gluconeogenesis, as well
 as changes in lipid, carbohydrate and protein metabolism. Activation of the
 hypothalamus-pituitary-adrenal axis, coagulation and compliment pathways is also one of the
 salient features of the acute phase response. It is regulated by cytokine receptor
 antagonists (IL-1Ra) and soluble cytokine receptors. Cytokines are soluble, low molecular
 weight glycoproteins that act as inflammatory mediators and can be broadly divided into pro
 and anti-inflammatory cytokines. At low concentrations, they have a paracrine effect, while
 in high concentrations they act systemically. These pro-inflammatory cytokines (IL-1, IL-2,
 IL-6 and tumor necrosis factor (TNF) alpha) remain in the plasma for at least 24 hours after
 generation. They also attract leukocytes (neutrophils and macrophages) to the site of injury
 and are detrimental if produced in large amounts. Oka et al have demonstrated that increasing
 levels of IL-6 are directly related to an increased rate of postoperative complications.

 In low doses, GC reduce short term mortality, reverse the shock process and improve
 hemodynamics in septic shock patients. In a meta-analysis of 17 RCTs, Annane et al found that
 28-day mortality for a low-dose dexamethasone (DXA) group, versus control group was 35.3% vs.
 38.5%. Shock reversal was also increased in the DXA group (66.9% vs. 58.6%) and the ICU stay
 was decreased by 4.49 days.

 Preoperative use of GC improves cardiac functions after coronary artery bypass grafting. The
 likely underlying mechanism for this cardio-protective effect is reduced release of
 pro-inflammatory cytokines IL-6, IL-8, and TNF-alpha with a concurrent increased release of
 anti-inflammatory cytokine IL-10.

 In cases of laparoscopic cholecystectomy, 50% to 75% of patients express nausea and vomiting
 in the early postoperative period. In a meta-analysis, Karanicolas et al found that the
 absolute risk of nausea in low risk patients. Similarly DXA decreases the absolute risk of
 vomiting from 12% to 5% in low risk and 30% to 12% in high risk groups as compared to
 placebo. Although the quality of evidence is low regarding postoperative pain in
 post-cholecystectomy patients, researchers have found less postoperative pain in the DXA
 group.

 Gomez-Hernandez et al also suggested that a preoperative single dose of DXA decreased
 postoperative nausea, vomiting and pain in patients undergoing mastectomy (28.6% vs. 60% in
 the early postoperative period). The patients in placebo group required more anti-emetic
 medications. DXA reduced postoperative pain significantly and fewer patients requested
 analgesia (10 vs. 21). Additionally there was no increase in adverse events, morbidity and
 mortality in the steroid group.

 Preoperative use of DXA significantly improves postoperative pain, nausea, vomiting and vocal
 function within the first 48 hours after thyroid surgery.

 Subclinical renal dysfunction is frequently noted following liver transplantation. This
 occurs mainly due to hepatic ischemia/reperfusion injury. Turner et al suggested that GC
 decrease the incidence of renal tubular dysfunction when given at the time of induction of
 anesthesia. The main difference in serum creatinine level was noted on postoperative day 3
 (164.8 mumol/L in the saline group vs. 88.5 mumol/L in the MP group). Additionally, patients
 in the saline group suffered from more complications (47% vs. 12%).

 Studies have suggested that GC limit the peritoneal inflammatory response and therefore help
 in recovery and fatigue in the early postoperative period. Low levels of IL-6 and IL-13 were
 found in the peritoneal fluid of patients receiving GC on postoperative day 1. Schulze et al
 observed improved pulmonary function and early mobilization in a MP group, as compared to
 placebo, in colon surgery patients. Furthermore, they noted a significant decrease in IL-6, C
 reactive protein and pain scores. The appetite was increased significantly in patients on GC,
 however there was no difference in wound healing and other infectious complications
 (including anastomotic leaks). In colorectal surgery, GC do not appear to increase
 postoperative complications. A double blind, randomized clinical trial consisting of 30
 patients undergoing colonic surgery, showed that there was no significant difference in
 postoperative IL-6, C reactive protein, pain scores, bowel functions, mobilization, hospital
 length of stay, complications and readmission rates between the two groups when a single 8 mg
 preoperative dose of DXA was given to the interventional group.

 A recent systematic review and meta-analysis of 11 moderate quality RCTs suggested decreased
 postoperative complications and hospital length of stay of patients undergoing major
 abdominal surgery following preoperative use of GC. In the case of liver resection surgery,
 Aldrighetti et al found decreased serum ALT, AST, total bilirubin, IL-6, TNF-alpha, hospital
 length of stay and postoperative complications following a single preoperative GC dose.

 The dominant clinical rationale for not using preoperative steroids is to avoid related side
 effects. A substantial number of studies have documented fewer adverse events in MP groups as
 compared to placebo, suggesting there is a need to reconsider the steroid-free protocol in
 the preoperative period. Further appropriately designed and well-powered studies are urgently
 needed to evaluate the effects of preoperative GC use. As a result, we have planned a
 prospective RCT at the University of Calgary to determine the role of GC in reducing
 postoperative complications and hospital length of stay for patients undergoing partial
 hepatectomy.

 Recruitment:

 Contact information for study candidates will be obtained by the project's research staff
 from the offices of the four participating surgeons. Patients will be contacted prior to
 surgery by the research staff and will be provided information regarding the study. Patients
 who consent to participate will be randomized (the clinic at the time of consent completion
 and operative scheduling).

 Data collection:

 Patients will be tracked postoperatively for the development of complications and overall
 length of hospital stay. Information will be collected by chart review from the operative
 report and patient record by research staff.

 Data elements to be collected will include, but not be limited to:

 -  Date of birth, Gender

 -  Surgeon, procedure, surgery duration

 -  Antibiotic prophylaxis

 -  Estimated blood loss, Transfusion

 -  Wound closure technique, peroperative drain, Wound infection or disruption

 -  Hepatic failure

 -  Acute kidney injury

 -  Intraperitoneal abscess, surgical site infection

 -  Pneumonia, Blood stream infection, Pulmonary embolism, Deep venous thrombosis

 -  Hypoglycemic incidents

 -  Bile leak (requiring percutaneous drainage or via an operatively placed drain)

 -  Anastomotic gastrointestinal leaks (combined colorectal / hepatic resection)

 -  Preparation type (ETOH based, chlorhexidine or iodine based)

 -  Time to oral intake

 Patient records:

 -  Preoperative chemotherapy

 -  Preoperative albumin level

 -  Preoperative PVE and /or TACE

 -  Underlying liver disease (cirrhosis)

 -  ASA score

 -  Length (if any) of hepatic inflow occlusion,

 -  Length of hospital stay & mortality

 -  Co-morbidities (smoking, diabetes, hypertension, COPD, Ischemic heart disease,
 arrhythmias, metastatic cancer, AIDS, obesity, alcohol abuse,psychosis, anemias etc.)

 -  Glucocorticoids - methylprednisolone given, dose, route of administration and time
 before starting surgery.

 The investigators will also track IL-6 and C-Reactive protein (CRP) levels on postoperative
 day 1 (with daily blood work) as a marker of global immune function. Definition of
 complications can be found in appendix 2.

 Power calculation to determine sample size:

 The largest surgical trial to date recruited a total of 73 patients. There are 5 small
 hepatic resection RCTs available for analysis (all trials utilized MP). The largest of these
 studies noted a decrease in overall complications from 54% to 14% with GC use. With a
 consistent volume of hepatectomies (100+ per year at FMC), and a planned inclusion/capture
 for our group's resections of nearly 100%, we expect to randomize over 100 patients in each
 arm in 2 full years. With an alpha = 0.5, probability = 80%, predicted effect size of 0.30
 (2-tailed test), the sample size required would be 84 patients.

 Additional comments:

 Methylprednisolone (MP) is the best choice of GC because it: (1) has a 5-fold higher
 anti-inflammatory action compared to cortisol, (2) has reduced effects on electrolytes, (3)
 duration of MP biologic action is 36 hrs (half life = 2.8 hrs) so both phases of
 ischemia-reperfusion injury will be covered, and (4) 500 mg of MP results in a defined and
 predictable response of >1 ug/ml in blood and >10 ug/ml in liver tissue.

 Resources:

 This project is built on an existing research team who work collaboratively with the
 Principal Investigator to carry out the project effectively and efficiently. An experienced
 research assistant will assist in data collection, analysis, preparing reports for
 publications and organizing research meetings.

 Appendix 2:

 Hepatic failure; Hyperacute and/or acute onset of coagulopathy (INR>1.5), reduction in
 synthetic function, and encephalopathy requiring therapy.

 Acute kidney injury; An abrupt (<48 hrs) reduction in kidney function (absolute increase in
 serum creatinine > or equal to 26.4 umol/L, a > 50% increase in serum creatinine or a
 reduction in urine output of less than 0.5 ml/kg per hour for more than six hours.

 Intraperitoneal abscess; Identified on cross-sectional imaging. Bile leak; Either requiring
 percutaneous drainage or evidence of bile in an operatively placed closed suction drain.

 Wound disruption, dehiscence or hernia Pneumonia; Blood stream infection; Urinary tract
 infection; Surgical site infection.

 Anastomotic gastrointestinal leaks (combined colorectal/hepatic resections); GI leak
 confirmed by imaging (fistulogram, CT) and requiring intervention.

 Deep venous thrombosis; Identified on ultrasonography or cross sectional imaging prompted by
 clinician suspicion or incidental imaging.

 Pulmonary embolus; Identified on cross-sectional or V/Q scan imaging. Hypoglycemic incidents;
 Blood glucose <3.0 mm/L with concurrent patient symptoms of hypoglycemia.
Inclusion Criteria:

 -  Elective surgery patients (adults) scheduled to undergo hepatectomy at FMC from May
 2013 to May 2015.

 Exclusion Criteria:

 -  Patients will be excluded if they are unable to give informed consent.