Circulating Non-coding RNA in Acute Ischemic Stroke (AISRNA)
Circulating Non-coding RNA in Acute Ischemic Stroke (AISRNA)
AISRNA is to analyze the expression pattern of circular RNA (circRNA), micro-RNA (miRNA) and
 long non-coding RNA (lncRNA) by next-generation sequencing in patients with acute ischemic
 stroke and healthy control. The candidate circRNA/miRNA/lncRNA will be verified as biomarkers
 for the detection and prognosis of acute ischemic stroke.
Noncoding RNAs have been highlighted to be involved in the pathological process of ischemic
 stroke (IS). The purpose of this protocol will investigate the expression pattern of circular
 RNA (circRNA), micro-RNA (miRNA) and long non-coding RNA (lncRNA) by next-generation
 sequencing in patients with acute ischemic stroke and healthy control. The candidate
 circRNA/miRNA/lncRNA will be verified as biomarkers for the detection and prognosis of acute
 ischemic stroke. Distinctive expression patterns of circRNA/miRNA/lncRNA will be identified
 by the next-generation sequencing and individual quantitative real time polymerase chain
 reaction (qRT-PCR). A diagnostic or predictive model will be established using logistic
 regression. The panel of these altered ncRNAs may be associated with acute IS and could serve
 as a novel diagnostic or predictive method.
Inclusion Criteria:

 -  Aged 18 years or older

 -  Confirmed acute ischemic stroke by a diffusion-weighted imaging-position lesion on
 magnetic resonance imaging (MRI) and a new lesion on a brain computed tomography (CT)
 scan

 -  Within 72 hours of symptom onset

 -  Good performance status

 -  Signed an approved informed consents

 Exclusion Criteria:

 -a history of hemorrhagic infarction, chronic kidney/liver diseases, peripheral arterial
 occlusive disease, active malignant disease, and inflammatory or infectious diseases