Induction Chemotherapy Using Cyclophosphamide and Topotecan in Treating Patients Who Are Undergoing Autologous Peripheral Stem Cell Transplantation for Newly Diagnosed or Progressive Neuroblastoma
Induction Chemotherapy Using Cyclophosphamide and Topotecan in Treating Patients Who Are Undergoing Autologous Peripheral Stem Cell Transplantation for Newly Diagnosed or Progressive Neuroblastoma
RATIONALE: Drugs used in chemotherapy, such as topotecan and cyclophosphamide, use different
 ways to stop tumor cells from dividing so they stop growing or die. Combining chemotherapy
 with autologous stem cell transplantation may allow the doctor to give higher doses of
 chemotherapy drugs and kill more tumor cells.

 PURPOSE: This phase I trial is studying the side effects of induction chemotherapy using
 cyclophosphamide and topotecan in treating patients who are undergoing surgery and autologous
 stem cell transplantation followed by radiation therapy for newly diagnosed or progressive
 neuroblastoma.
OBJECTIVES:

 Primary

 -  Determine the toxicity and feasibility of adding cyclophosphamide and topotecan to
 induction therapy in patients with newly diagnosed or progressive high-risk
 neuroblastoma undergoing autologous peripheral blood stem cell (PBSC) transplantation.

 -  Determine the feasibility of PBSC mobilization and in vivo PBSC tumor purging in these
 patients after treatment with this regimen.

 Secondary

 -  Determine tumor response rate in patients treated with this regimen.

 -  Determine the pharmacokinetics of this regimen in these patients.

 -  Determine whether topotecan affects cyclophosphamide pharmacokinetics in these patients.

 -  Correlate host DNA with toxicity and cyclophosphamide and topotecan pharmacokinetics in
 patients treated with this regimen.

 -  Determine toxicity in patients treated with this regimen.

 OUTLINE: This is a pilot, multicenter study. Patients are stratified according to diagnosis
 (newly diagnosed vs initially stage 1, 2, or 4S that progressed to stage 4 without interval
 chemotherapy).

 -  Induction therapy: Patients receive 6 courses of induction therapy.

 -  Courses 1 and 2: Patients receive cyclophosphamide IV over 30 minutes and topotecan
 IV over 30 minutes on days 1-5 and filgrastim (G-CSF) subcutaneously (SC) or IV
 beginning on day 6 and continuing until blood counts recover.

 -  Course 3: Patients receive etoposide IV over 2 hours on days 1-3, cisplatin IV over
 1 hour on days 1-4, and G-CSF SC or IV beginning on day 5 and continuing until
 blood counts recover.

 -  Course 4: Patients receive cyclophosphamide IV over 6 hours on day 1 and
 doxorubicin IV and vincristine IV continuously over 24 hours on days 1-3. Patients
 also receive G-CSF SC or IV beginning on day 4 and continuing until blood counts
 recover.

 -  Course 5: Patients receive etoposide, cisplatin, and G-CSF as in course 3.

 -  Course 6: Patients receive cyclophosphamide, doxorubicin, vincristine, and G-CSF as
 in course 4.

 Treatment repeats every 21 days for a total of 6 courses in the absence of disease
 progression or unacceptable toxicity.

 -  Consolidation therapy: Within 4-6 weeks after completing induction therapy, patients
 receive melphalan IV on days -7 to -5 and etoposide IV and carboplatin IV continuously
 over 24 hours on days -7 to -4.

 -  Stem cell transplantation: Peripheral blood stem cells are collected after course 2 of
 induction therapy and infused on day 0. Patients receive G-CSF IV beginning on day 0 and
 continuing until blood counts recover.

 -  Surgery: After course 5 of induction therapy, patients undergo surgery.

 -  Radiotherapy: Beginning 28-42 days after transplantation, patients receive 12 fractions
 of local radiotherapy to all areas of residual soft tissue disease and the primary tumor
 site, even if completely resected.

 -  Maintenance therapy: Beginning 66 days after transplantation, patients receive oral
 isotretinoin twice daily on days 1-14. Treatment repeats every 28 days for a total of 6
 courses.

 Patients are followed every 3 months for 1 year, every 6 months for 4 years, and then
 annually thereafter.

 PROJECTED ACCRUAL: A total of 10-29 patients will be accrued for this study within 2 years.
DISEASE CHARACTERISTICS:

 -  Histologically or cytologically confirmed neuroblastoma or ganglioneuroblastoma
 meeting 1 of the following staging criteria:

 -  Newly diagnosed disease, at least 1 year of age, and meets criteria for 1 of the
 following:

 -  International Neuroblastoma Staging System (INSS) stage 2a/2b with MYCN
 amplification (greater than 10) AND unfavorable pathology

 -  INSS stage 3 with MYCN amplification OR unfavorable pathology

 -  Newly diagnosed INSS stage 4 disease meeting criteria for 1 of the following:

 -  Over 18 months of age

 -  Age 12 to 18 months with any unfavorable biologic feature (MYCN
 amplification, unfavorable pathology, and/or DNA index=1) or any biologic
 feature that is indeterminant, unsatisfactory, or unknown

 -  No INSS stage 4 disease and age 12 to 18 months with all 3 favorable
 biologic features (i.e., nonamplified MYCN, favorable pathology, and
 DNA index greater than 1)

 -  Newly diagnosed INSS stage 3, 4, or 4S disease AND under 1 year of age with MYCN
 amplification

 -  At least 1 year of age and initially diagnosed with INSS stage 1, 2, or 4S
 disease that progressed to stage 4 without interval chemotherapy

 -  Must have been enrolled on COG-ANBL00B1 at initial diagnosis

 PATIENT CHARACTERISTICS:

 Age

 -  30 and under at initial diagnosis

 Performance status

 -  Not specified

 Life expectancy

 -  Not specified

 Hematopoietic

 -  Absolute neutrophil count at least 1,000/mm^3*

 -  Platelet count at least 100,000/mm^3* (transfusion independent)

 -  Hemoglobin at least 10.0 g/dL* (red blood cell transfusions allowed) NOTE:
 *Granulocytopenia, anemia, and/or thrombocytopenia allowed for patients with tumor
 metastatic to the bone marrow

 Hepatic

 -  Bilirubin no greater than 1.5 mg/dL

 -  ALT less than 300 IU/L

 Renal

 -  Creatinine no greater than 1.5 mg/dL

 -  Creatinine clearance or radioisotope glomerular filtration rate at least 60 mL/min

 Cardiovascular

 -  ECG normal

 -  Shortening fraction at least 27% by echocardiogram OR

 -  Ejection fraction at least 50% by MUGA

 Other

 -  Not pregnant or nursing

 -  Negative pregnancy test

 -  Fertile patients must use effective contraception

 -  HIV negative

 PRIOR CONCURRENT THERAPY:

 Biologic therapy

 -  Not specified

 Chemotherapy

 -  See Disease Characteristics

 -  No more than 1 prior chemotherapy course on the low- or intermediate-risk
 neuroblastoma studies (COG-P9641, COG-A3961) prior to determination of MYCN
 amplification and Shimada histology

 Endocrine therapy

 -  Not specified

 Radiotherapy

 -  Prior localized emergency radiotherapy to sites of life-threatening or
 function-threatening disease allowed

 Surgery

 -  Not specified

 Other

 -  No other prior systemic therapy