Relationship Between Polymorphism of Heat Shock Protein 70 Gene and Hepatocellular Carcinoma
Relationship Between Polymorphism of Heat Shock Protein 70 Gene and Hepatocellular Carcinoma
Polymorphisms of HSP70 and tumor necrosis factor-alpha promoter in patients with
 hepatocellular carcinoma, chronic liver disease and healthy controls will be measured by
 PCR-RFLP or direct sequencing. The clinical relevance of patients will be compared in those
 with polymorphism and those without.
Heat shock protein 70 (HSP 70) may be involved in various aspects of immune system including
 infection, autoimmunity, and tumor immunity. The relationship between HSP70 expression,
 disease activity and cancer prognosis varied according to type of cancer. The overexpression
 of HSP70 by tumor cells contributes to tumor behavior. HSP70 is present in normal and
 abnormal hepatocytes, and was reported to be over-expressed in both hepatoma cell lines and
 human hepatocellular carcinoma (HCC) tissue. Recent study indicated that HSP70 is a molecular
 marker during early hepatocarcinogenesis. It is also involved in anti-tumor immunity in
 HCC.We also investigate the polymorphism of tumor necrosis factor-alpha promoter, and
 correlated to clinical relevance.
Inclusion Criteria:

 -  History proven HCC patients

 Exclusion Criteria:

 -  No-definite diagnosed patients