Cortisol Levels on Menopausal Symptoms - Ancillary (Addendum) Study to Protocol 16997
Cortisol Levels on Menopausal Symptoms - Ancillary (Addendum) Study to Protocol 16997
This is an ancillary study to Protocol 16997 to examine the stress hormone level in women
 with menopausal symptoms. This ancillary project will collect additional data from the 45
 subjects enrolled in Protocol 16997 (no subjects have enrolled in Protocol 16997 so far).
 This study protocol differs from the main protocol (16997) because it will include an ACTH
 (Hormone) Stimulation Test which will assess the functioning of stress response in the
 subjects at entry and exit.

 Subjects will undergo the ACTH test for this ancillary protocol in the morning when they
 complete 24-hour urine collection for Protocol 16997. The ACTH (Hormone) Stimulation Test
 will not be done in the main protocol 16997. During the ACTH test an IV will be placed in
 subjects arms, 25mL of blood will be drawn at -30 minutes and 6mL of blood will be drawn at
 -15 minutes and 0 minutes respectively. Following, 0.25mg of ACTH will be injected
 intravenous over 60 seconds, and a final 12mL of blood will be drawn 60 minutes later. That
 is, a total of 50mL of blood will be drawn to test serum blood hormone levels from the
 subjects during the ACTH (Hormone) Stimulation Test.

 The 24 hour cortisol data collected in the main protocol 16997 will be used for this study
 analysis.

 When subjects are consented for Protocol 16997, they will also be consented for this
 ancillary study. In addition to the consent visit, subjects will have two study specific
 visits (entry and exit) for this ancillary study. The PI will schedule the specific visits
 for this study to be in conjunction with those scheduled for Protocol 16997.

 There is no collaborations with other sites in the ancillary study.
Inclusion Criteria:

 1. Women with menopausal VMS bothersome enough to warrant treatment;

 2. Minimum of 7 hot flashes per day (on average);

 3. Age limits: women ages 40-70 who have had at least one missed menstrual cycle or have
 undergone spontaneous menopause, women of any age who have medically induced
 menopause, women of any age who have had oophorectomy;

 4. Informed written consent;

 5. Ability to follow treatment protocols.

 Exclusion Criteria:

 Exclusion Criteria (cohort)

 1. Concomitant illness with reasonable likelihood of limiting survival to less than one
 year;

 2. Current substance abuse (alcohol or drug);

 3. Pregnancy known, suspected or planned in next year.

 Exclusion Criteria (TA intervention)

 1. Other concomitant menopause treatment;

 2. Participating in acupuncture treatment or formal psychological stress management
 program within the last year;

 3. Participating in another treatment for VMS, unless willing to stop it 4 weeks in
 advance of participation;

 4. HIV infection, chronic or active hepatitis or other blood-borne illness.