Follow-up of Girls With Premature Thelarche and Precocious Puberty
Follow-up of Girls With Premature Thelarche and Precocious Puberty
Precocious puberty - the onset of signs of puberty before 8 years of age in girls - is be
 physically and emotionally devastating for kids and is sometimes caused by an underlying and
 medical condition. One sign of precocious puberty in small girls is breast development
 (thelarche). This can persist without other physical changes of puberty, it may continue into
 precocious puberty, or it may disappear. This project will improve the diagnostics of
 precocious puberty in girls and improve our ability to identify which girls with thelarche,
 who will develop precocious puberty and need medical treatment.
The purpose of the study is

 1. To conduct an epidemiological study in which characteristics of 0-6 year-old girls with
 either premature thelarche or precocious puberty are described (as specified below)

 2. To conduct a clinical and para-clinical re-examination of the before-mentioned (1))0-6
 year-old girls

 3. To compare the clinical and paraclinical parameters in point 1) mentioned 0-6 year-old
 girls with a control group of children at the same age, whiteout thelarche and
 precocious puberty. (Local Ethics Committee has approved the study of the control group,
 case number 1-10-72-631-12.)

 The study includes a chart review of registered 0-6 year-old girls diagnosed with premature
 thelarche or precocious puberty from 1998 and until today at Dept. of Pediatrics A, AUH
 Skejby. The cohort has not earlier been examined and the number of girls in the cohort is
 estimated from our clinical experience with this patient group to be about 50 in total. The
 following characteristics from the patients records will be registered in a database:

 Age at diagnosis, anthropometry (height and weight), Tanner stage - breast, Tanner stage -
 pubic hair, GnRH test (LH and FSH response), thyroid parameters, estrogen, prolactin, 17OHP,
 androstenedione, dihydroepiandrosteron (DHEA), testosterone, inhibin B, SHBG, AFP,
 kisspeptin, human chorionic gonadotropin (HCG), bone age, MRI of the cerebrum, US of internal
 genitalia, and exposures to environmental chemicals known to affect secondary sex
 characteristics.

 The database will be developed in the program Access. The results will be reported as mean
 and standard deviation and minimum and maximum values for each age group by Tanner stage, if
 the data are normally distributed. Non-normally distributed data will be given as median and
 confidence intervals as well as minimum and maximum values for each Tanner stage. Data for
 previously studied children with suspected precocious puberty, will be reported in the same
 way. Statistical comparison between the groups will be made with u-paired t-test or
 equivalent non-parametric test, if the data is not normally distributed.

 The Data Unit at Aarhus University Hospital, Skejby, identifies 0-6 year-old girls with
 diagnoses premature thelarche or precocious puberty in 1998 and onwards. Girls below 8 years
 of age at baseline of the project (1st of September 2013) will be contacted and requested to
 participate in a re-examination.

 The follow-up is a clinical and paraclinical re-examination, where the following parameters
 are measured: anthropometry (height and weight), skinfold thickness, Tanner stage, bone age,
 GnRH test, p-estrogen, p-testosterone , p-SHBG, p- androstenedione, p-dihydroandrostendion,
 p-inhibin B, p-thyroid parameters, ultrasound examination of the genitalia interna and a
 blood sample for studies of polymorphism in the estrogen receptor gene and aromatase enzyme
 gene.

 If the girls are unable or unwilling to attend the reexamination, they are requested to fill
 out a questionnaire instead as follows:

 -  name, social security number, date of completion the questionnaire

 -  current height, current weight, birth weight, origin

 -  date of onset of puberty if started, current pubertal development, menarche

 -  diseases/admissions

 Regarding the mother:

 - age at menarche, medicine during pregnancy/breastfeeding, diet during
 pregnancy/breastfeeding, use of cosmetics /cream/shampoo during pregnancy,

 Regarding the family:

 - others with early breast development/early puberty

 The project is conducted at Dept. of Pediatrics A, Aarhus University Hospital, Skejby,
 Skejbygårdsvej 100, 8200 Aarhus N. The other pediatric wards in the Region of Midtjylland
 (located in Randers, Viborg and Herning) will be included in the project if they prefer to
 participate.
Inclusion Criteria:

 -  Clinical diagnosis of precocious puberty or premature thelarche

 -  0-6 year old girls at time of diagnosis for the medical record review

 -  0-8 year old girls at baseline for the re-examination

 Exclusion Criteria:

 -