Interleukin-2 Following 4SCAR19/22 T Cells Targeting Refractory and/or Recurrent B Cell Malignancies
Interleukin-2 Following 4SCAR19/22 T Cells Targeting Refractory and/or Recurrent B Cell Malignancies
Clinical studies of CD19 CAR-T cells in the treatment of blood and lymphatic system tumors
 have achieved unprecedented successes. Because of the heterogeneity of the tumor, patients
 often carry CD19-negative tumor cell clones that express alternative target antigens (such as
 CD22, CD20 and CD123). In order to effectively eradicate all tumor clones and prevent
 recurrence, alternative tumor antigens besides CD19 are considered for CAR-T cell targeting.
 In this tudy, autologous T cells are genetically modified with 4th generation anti-CD19 and
 anti-CD22 CARs (4SCAR19/22) using lentiviral vectors. For safety consideration, the 4SCAR is
 engineered with an inducible caspase 9 self-withdrawal genetic design that allows for rapid
 elimination of the infused CAR-T cells. Interleukin-2 has been shown to boost immune response
 against leukemia cells. The serum interleukin-6 level will be monitored and when it returns
 to normal range by day 28 after CAR-T cell infusion, patients will receive subcutaneous
 injection of interleukin-2, and evaluated for 24 months for safety, efficacy and persistence
 of CAR T cells.
Objectives:

 1. Primary Outcome Measures:

 1. Evaluate the frequency and severity of adverse events, including, but not limited
 to, cytokine release syndrome (CRS) [ Time Frame: From date of dosing ( day 1 ) up
 to 50 weeks ]

 2. Evaluate grade 3 and higher toxicity rate of patients (toxicity possibly attributed
 to 4SCAR19/22 T cells)

 3. Evaluate the safety and effect of administration of 4SCAR19/22 T cells followed by
 interleukin-2 in treating pediatric patients with relapsed and refractory B cell
 malignancies.

 2. Secondary:

 1. Evaluate Overall Complete Remission Rate (ORR).

 2. Evaluate overall response rate including complete remission (CR) and complete
 remission with incomplete blood count recovery (CRi) .

 3. Evaluate duration of remission (DOR).

 4. To evaluate the incidence and the treatment effect of cytokine release syndrome
 (CRS).

 5. To determine the expansion and functional persistence of 4SCAR19/22 T cells in the
 peripheral blood of patients and the correlation with antitumor effects;

 Design:

 1. In this single-center, open-label, nonrandomized, no control, prospective clinical
 trial, a total of 30 patients with resistant or refractory B cell acute lymphoblastic
 leukemia (ALL) or non-hodgkin's lymphoma (NHL) will be enrolled. Patients with ALL will
 be diagnosed according to bone marrow morphology, immunophenotype, cytogenetic and
 molecular examination. Patients with NHL will be diagnosed according to bone marrow
 morphology, biopsy pathology and imaging examination.

 2. Peripheral blood mononuclear cells (PBMC) will be obtained by apheresis, and T cells
 will be activated and modified to express the 4SCAR-CD19/22 gene.

 3. On Day -2 to -7, PBMC will be activated and enriched for T cells, which will be followed
 by 4SCAR-CD19/22 lentiviral transduction. The total culture time is approximately 5-7
 days.

 4. Patients will receive lymphodepleting chemotherapy composed of cyclophosphamide and
 fludarabine prior to cell infusion,

 5. Participants will receive the CAR-T cells at a total dose of 0.5-5x10^6/kg.

 6. Participants will receive regular subcutaneous injection of interleukin-2 of
 250,000iu/m^2 every other day for 2 weeks and then rest for 2 weeks for up to 6 months
 after their serum interleukin-6 levels returned to normal range from day 28 after CAR-T
 cell infusion.

 7. Patients will be monitored for toxicity including cytokine release syndrome, hematologic
 toxicities and B-cell aplasia, for response of their underlying malignancy and for CAR-T
 cell persistence in the blood, marrow and cerebral spinal fluid (CSF).
Inclusion Criteria:

 1. Relapsed or refractory CD19+ B-cell lymphoma or leukemia.

 2. Measurable disease.

 3. Karnofsky/jansky score of 60% or greater.

 4. ≥1 years old and ≤14 years.

 5. Fertile females/males.

 6. Expected survival>12 weeks.

 7. Histologically confirmed as CD19/20-positive ALL/NHL and who meet one of the following
 conditions:

 1. Patients receive at least 2-4 prior combination chemotherapy regimens (not
 including single agent monoclonal antibody therapy) and fail to achieve CR.

 2. Recurrent disease and not eligible for allogeneic stem cell transplantation, and
 stable disease after therapy but refused further treatment.

 3. Disease recurrence after stem cell transplantation.

 4. Diagnosis as lymphoma, and refuse conventional treatment such as chemotherapy,
 radiation, stem cell transplantation and monoclonal antibody therapy.

 8. Creatinine < 2.5 mg/dl.

 9. Alanine transaminase (ALT) <3x upper limit of normal (ULN), aspartate aminotransferase
 (AST) <3x ULN.

 10. Bilirubin < 2.0 mg/dl.

 11. Adequate venous access for apheresis, and no other contraindications for
 leukapheresis.

 12. Take contraceptive measures before recruit to this trial.

 13. Written voluntary informed consent is given.

 Exclusion Criteria:

 1. A history of mental illness and poorly controlled.

 2. Patients with symptoms of central nervous system.

 3. Suffering severe cardiovascular or respiratory disease.

 4. Accompanied by other malignant tumor.

 5. Known human immunodeficiency virus (HIV) infection.

 6. Active and/or severe infection (e.g. tuberculosis, sepsis and opportunistic
 infections, active hepatitis B virus (HBV) or active hepatitis C virus (HCV)
 infection).

 7. Other serious underlying medical conditions, which, in the Investigator's judgment,
 could impair the ability of the patient.

 8. Taking immunosuppressive agents within 1 week due to organ transplantation or other
 disease which need long-lasting administration.

 9. Patients that do not consent to tissue and blood sample collection and storage in a
 biobank.

 10. Female study participants of reproductive potential must have a negative serum or
 urine pregnancy test performed 48 hours before infusion.

 11. Pregnancy and nursing females.