Exhaled Breath Analysis by Secondary Electrospray Ionization - Mass Spectrometry in Children and Adolescents
Exhaled Breath Analysis by Secondary Electrospray Ionization - Mass Spectrometry in Children and Adolescents
This study is to investigate breath analysis (breath metabolomics) combined with established
 bioinformatic tools as a platform for companion diagnostics.
Therapeutic drug monitoring (TDM) is defined as measuring concentrations of a drug at one or
 more time points in a biological matrix after a dose. The purpose of TDM is to individualize
 the drug dose to achieve maximum efficacy and at the same time minimize toxicity. The concept
 of TDM could potentially be even more valuable if in addition to drug concentrations, other
 drug-regulated and drug-related metabolites could be included in the models to define optimal
 dosage. There exists a clinical need to stratify patients with better precision to improve
 current clinical and therapeutic management. Breath analysis offers an opportunity to
 non-invasively retrieve relevant information on the ongoing internal biochemical processes,
 as well as to monitor the respiratory system itself. For breath analysis, a Secondary
 Electrospray ionization - mass spectrometry (SESI-MS) breath analysis platform will be used
 to capture disease-related, drug-regulated and drug-related metabolites (breath metabolomics)
 in exhaled breath. This information, retrieved in parallel to standard of care clinical
 co-variates, could have the potential to provide a more personalized therapeutic management
 of patients.
Inclusion Criteria:

 -  Age 0 ≤ 22 years at study entry and signed informed consent

 Additional inclusion criteria for respiratory disease population:

 -  Acute disease: - Acute signs for a respiratory disease, indicated by e.g. increased
 work of breathing (e.g. dyspnea, increased respiratory rate), cough or wheezing.

 -  Chronic disease: - Suspected or confirmed chronic airway disease (e.g. asthma).

 Additional inclusion criteria for neurological disease population:

 -  Acute disease: - Acute presentation or report within 24 hours of any signs of
 neurological deficit (motor function, sensoneural, or verbal).

 -  Chronic disease: - Confirmed chronic neurologic disease (e.g. childhood epilepsy).

 Additional inclusion criteria for T1D disease population:

 -  Acute disease: - Hyperglycemia and/or pH (venous) <7.3, bicarbonate >10 mmol/L,
 increased levels of acetone in blood or urine in the context of newly diagnosed or
 known T1D.

 -  Chronic disease: - Confirmed diagnosis of T1D

 Exclusion Criteria:

 -  Physical or intellectual impairment precluding protocol adherence.

 Additional exclusion criteria for respiratory disease population:

 -  Known malignancy, active smoker (passive smoke exposure is not an exclusion
 criterium), known inflammatory diseases (e.g. autoimmune disease) that require medical
 and/or pharmacological treatment and is associated with an inflammatory response,
 relevant congenital defects

 Additional exclusion criteria for neurological disease population:

 -  Known malignancy, active smoker (passive smoke exposure is not an exclusion
 criterium), known inflammatory diseases (e.g. autoimmune disease) that require medical
 and/or pharmacological treatment and is associated with an inflammatory response,
 relevant congenital defects.

 Additional exclusion criteria for T1D population:

 -  Known malignancy, active smoker (passive smoke exposure is not an exclusion
 criterium), relevant congenital defects.