Testing the Addition of an Anti-cancer Viral Gene Therapy, Toca 511/Toca FC, to the Usual Treatment (Temozolomide and Radiation Therapy) for Newly Diagnosed Glioblastoma
Testing the Addition of an Anti-cancer Viral Gene Therapy, Toca 511/Toca FC, to the Usual Treatment (Temozolomide and Radiation Therapy) for Newly Diagnosed Glioblastoma
This phase II/III trial studies how well vocimagene amiretrorepvec (Toca 511) and extended
 release flucytosine (Toca FC) work when added to the usual treatment (temozolomide and
 radiation therapy) in treating patients with newly diagnosed glioblastoma. Toca 511 is a live
 virus that has been built to carry a gene into tumor cells. This gene carries instructions
 that cause the tumor cells to turn Toca FC, typically used to treat fungal infections, into a
 drug that may kill the tumor cells. Drugs used in chemotherapy, such as temozolomide, work in
 different ways to stop the growth of tumor cells, either by killing the cells, by stopping
 them from dividing, or by stopping them from spreading. Radiation therapy uses high energy
 x-rays to kill tumor cells and shrink tumors. Giving Toca 511 and Toca FC in addition to the
 usual treatment (temozolomide and radiation therapy) may help shrink or stabilize cancer or
 extend the life of patients with newly diagnosed glioblastoma.
PRIMARY OBJECTIVES:

 I. To compare the progression-free survival (PFS) of patients with newly diagnosed
 glioblastoma treated with Toca 511 at the time of tumor resection followed by Toca FC in
 combination with standard of care (SOC) treatment to patients with newly diagnosed
 glioblastoma treated with SOC after tumor resection. (Phase II) II. To compare the overall
 survival (OS) from time of randomization of all patients with newly diagnosed glioblastoma
 treated with Toca 511 at the time of tumor resection followed by Toca FC in combination with
 SOC treatment to patients with newly diagnosed glioblastoma treated with SOC after tumor
 resection. (Phase III)

 SECONDARY OBJECTIVES:

 I. To evaluate the safety of each arm as administered in this study. II. To compare the OS
 between arms (only for phase II part of the study). III. To compare the PFS between arms
 using Response Assessment in Neuro-Oncology (RANO) (only for phase III part of the study).

 IV. To evaluate the objective response rate (ORR) in patients with measurable disease at the
 post-surgical scan.

 V. To evaluate the effect of IDH mutation status on survival outcomes between arms.

 VI. To evaluate the PFS and OS by extent of resection. VII. To evaluate the effect of MGMT
 methylation status on survival outcomes between arms.

 EXPLORATORY OBJECTIVES:

 I. To evaluate the duration of response (DoR) in patients with measurable disease at the
 post-surgical scan using mRANO.

 II. To evaluate the effect of corticosteroids administration on efficacy of Toca 511 and Toca
 FC.

 III. To optimize quality assurance methodologies and processes for radiotherapy and imaging.

 IV. To determine if there is a tumor-microenvironment signature based on ribonucleic acid
 (RNA) sequencing that is associated with a selective increase in benefit from the addition of
 Toca 511 and Toca FC.

 V. To compare regional genomic heterogeneity and molecular profiles, as measured by RNA
 sequencing and methylation analyses between arms.

 VI. To compare quality of life (QOL), as measured by the European Organization for Research
 and Treatment of Cancer Quality of Life Questionnaire-Core 30 (EORTC QLQ-C30) and Brain
 Cancer Module (BN20), between arms.

 VII. To compare health utilities, as measured by the EuroQoL 5-Dimension 5-Level (EQ-5D-5L),
 between arms.

 OUTLINE: Patients are randomized to 1 of 2 arms.

 ARM I: Beginning on week 5 following standard of care surgery, patients undergo radiation
 therapy over 30 fractions 5 days per week for up to 6 weeks, and receive temozolomide orally
 (PO) once daily (QD) for up to 49 days. At the discretion of treating physician, patients may
 also receive novoTTF-100A (Optune) device 0-7 weeks following radiation and temozolomide
 treatment. One month following completion of radiation therapy, patients continue to receive
 temozolomide PO QD on days 1-5. Treatment repeats every 28 days for up to 12 cycles in the
 absence of disease progression or unacceptable toxicity.

 ARM II: Patients receive vocimagene amiretrorepvec via intracranial injection during surgery
 on day 1. Beginning on week 5 following surgery, patients receive extended release
 flucytosine PO three times daily (TID) for 7 consecutive days every 7 weeks. Patients also
 undergo radiation therapy and receive temozolomide as in arm I. After completion of radiation
 therapy and at the discretion of the treating physician, patients may continue to receive
 extended release flucytosine PO TID for 7 consecutive days every 8 weeks in the absence of
 disease progression or unacceptable toxicity.

 After completion of study treatment, patients are followed up until progression then, every 3
 months for 1 year, every 4 months for 1 year, and then every 6 months thereafter.
Inclusion Criteria:

 -  Presumptive diagnosis of glioblastoma based on magnetic resonance imaging (MRI)
 imaging within 14 days prior to registration.

 -  NOTE: Patients who undergo treatment with Toca 511 whose final pathology shows
 diagnosis other than glioblastoma (e.g. anaplastic astrocytoma or
 oligodendroglioma or any other histology) will be treated with Toca FC and
 chemoradiation; however they will not be analyzed in the primary endpoint. The
 outcomes of these patients will be reported descriptively. Similarly the patients
 with anaplastic astrocytoma or oligodendroglioma or any other histology treated
 on the standard-of-care arm will be reported separately and they are allowed to
 receive the treatment per choice of the treating physician/ investigator (for
 e.g. radiation therapy [RT] plus temozolomide or RT plus
 procarbazine-lomustine-vincristine [PCV])

 -  In addition, patients who have undergone biopsy with diagnosis of glioblastoma and who
 have never received any chemotherapy and/or radiation and are candidates for >= 80%
 resection of enhancing region are eligible

 -  The tumor must be unifocal, confined to the supratentorial compartment and based on
 the pre-operative evaluation, the patient is a candidate for >= 80% resection of
 enhancing region

 -  Measurable disease preoperatively, defined as at least 1 contrast enhancing lesion,
 with 2 perpendicular measurements of at least 1 cm, as per Response Assessment in
 Neuro-Oncology (RANO) criteria

 -  The hematoxylin and eosin (H&E) slide and formalin-fixed paraffin-embedded (FFPE)
 tumor tissue block must be available to be sent for mandatory central pathology review
 after registration

 -  Patients must be able to undergo an evaluation by MRI within 96 hours post surgery to
 assess extent of resection

 -  Karnofsky performance status >= 70 within 14 days prior to registration

 -  History/physical examination within 14 days prior to registration

 -  Platelet count >= 100,000/mm^3 (within 14 days prior to registration)

 -  Hemoglobin (Hgb) >= 10 g/dL (within 14 days prior to registration)

 -  Absolute neutrophil count (ANC) >= 1,500/mm^3 (within 14 days prior to registration)

 -  Absolute lymphocyte count (ALC) >= 1000/mm^3 (within 14 days prior to registration)

 -  Total bilirubin =< 1.5 x upper limit of normal (ULN) (unless has Gilbert?s syndrome)
 (within 14 days prior to registration)

 -  Alanine aminotransferase (ALT) =< 2.5 x ULN (within 14 days prior to registration)

 -  Estimated glomerular filtration rate of at least 50 mL/min by the Cockcroft Gault
 formula (within 14 days prior to registration)

 -  Women of childbearing potential (women who have not had >=12 months of non therapy
 induced amenorrhea or are not surgically sterile) must have had a negative serum
 pregnancy test within 14 days prior to registration and must agree to use a birth
 control method in addition to barrier methods (condoms or diaphragm) during treatment
 with temozolomide

 -  If the patient is randomized to arm 2, the patient or patient?s partner must be
 willing to use barrier method of contraception for 12 months after receiving Toca 511
 and 1 month after stopping Toca FC or until there is no evidence of the virus in the
 patient?s blood, whichever is longer

 -  The patient or a legally authorized representative must provide study-specific
 informed consent prior to registration

 Exclusion Criteria:

 -  History of prior invasive malignancy (except non-melanomatous skin cancer) unless
 disease free for a minimum of 3 years

 -  A contrast enhancing brain tumor on MRI that is any of the following:

 -  Multi focal (defined as 2 separate areas of contrast enhancement measuring at
 least 10 mm in 2 planes that are not contiguous on either fluid attenuated
 inversion recovery [FLAIR] or T2 hyperintensity);

 -  Associated with either diffuse subependymal or leptomeningeal dissemination; or

 -  > 50 mm in any dimension

 -  Active infection (excluding skin or toenail infections) requiring systemic antibiotic,
 antifungal or antiviral therapy within 28 days prior to registration

 -  Bleeding diathesis, or must take anticoagulants, or antiplatelet agents, including
 nonsteroidal anti inflammatory drugs (NSAIDs), at the time of the scheduled resection
 that cannot be stopped for surgery

 -  Known human immunodeficiency virus (HIV) positive status

 -  History of allergy or intolerance to flucytosine

 -  Swallowing difficulty that would prevent patient from being able to swallow either
 temozolomide or Toca FC or severe active mal-absorption

 -  Patients who are breast feeding or lactating

 -  Intent to undergo treatment with the Gliadel wafer at the time of this surgery or has
 received the Gliadel

 -  Prior to registration, steroid treatment beyond a maximum of 8 mg/day of dexamethasone
 (or equivalent) or a total of 8 weeks (56 days) is excluded

 -  Severe pulmonary, cardiac or other systemic disease, specifically:

 -  New York Heart Association > =Class II congestive heart failure within 6 months
 (180 days) prior to registration, unless asymptomatic and well controlled with
 medication

 -  Uncontrolled or significant cardiovascular disease, clinically significant
 ventricular arrhythmia (such as ventricular tachycardia, ventricular
 fibrillation, or Torsades de pointes), clinically significant pulmonary disease
 (such as >= grade 2 dyspnea)

 -  Any other disease that as per investigator assessment may affect the patient?s
 compliance or place the patient at higher risk of potential treatment
 complications