A Study to Evaluate Single and Repeat Doses of IV GSK2251052 in Healthy Male Japanese and Caucasian Subjects and Repeat Doses of Supratherapeutic Doses of IV GSK2251052 in Healthy Volunteers
A Study to Evaluate Single and Repeat Doses of IV GSK2251052 in Healthy Male Japanese and Caucasian Subjects and Repeat Doses of Supratherapeutic Doses of IV GSK2251052 in Healthy Volunteers
This is a two-part study. Part A is a three-period study in approximately 24 healthy male
 Japanese and Caucasian subjects. Period 1 and Period 2 will be an open label study to
 investigate the safety, tolerability, and pharmacokinetics of single ascending intravenous
 doses of GSK2251052. Period 3 is a single blind, placebo controlled, repeat fixed dose design
 to evaluate the safety, tolerability and pharmacokinetics of multiple intravenous doses of
 GSK2251052 for 12 days. The selection of the repeat IV dose will be based on the results from
 Periods 1 and 2. Japanese subjects will be stratified based on their metabolic genotype,
 polymorphic or wild-type for ADH and ALDH. Caucasian subjects are not anticipated to have
 these enzyme polymorphisms and therefore will not be stratified.

 Part B is a two cohort, single-blind, randomized, placebo-controlled, dose-rising, repeat
 dose study in approximately 24 healthy male and female subjects to evaluate the safety,
 tolerability, and pharmacokinetics of supratherapeutic IV doses of GSK2251052 for 10 days.
 Cohort 1 subjects will be randomized to receive 2250 mg of GSK2251052 or placebo and Cohort 2
 subjects will be randomized to receive 3000 mg GSK2251052 or placebo. The decision to conduct
 Cohort 2 of Part B will be based on the available toxicology cover results from ongoing
 preclinical toxicity studies.
Inclusion Criteria:

 -  AST, ALT, alkaline phosphatase and bilirubin ≤ 1.5xULN (isolated bilirubin >1.5xULN is
 acceptable if bilirubin is fractionated and direct bilirubin <35%).

 -  Healthy adult male Japanese or healthy adult Caucasian male. In Part B, healthy adult
 males or females (Part B only) as determined by a responsible and experienced
 physician, based on a medical evaluation including medical history, physical
 examination, laboratory tests and cardiac monitoring. A subject with a clinical
 abnormality or laboratory parameters outside the reference range for the population
 being studied may be included only if the Investigator and the GSK Medical Monitor
 agree that the finding is unlikely to introduce additional risk factors and will not
 interfere with the study procedures. Subjects with coagulation, reticulocyte, or Hgb
 values outside the normal range should always be excluded from enrollment.

 -  Japanese defined as being born in Japan, having four ethnic Japanese grandparents,
 holding a Japanese passport or identity papers and being able to speak Japanese.
 Japanese subjects should also have lived outside Japan for less than 10 years.

 -  The following genetic variants of ADH and ALDH will be acceptable: Group 1 Japanese:
 ADH1B (*/1*1), ADH1B (*1/*2), ADH1B (*2/*2); ALDH2 (*1/*1). Group 2 Japanese: ADH1B
 (*/1*1), ADH1B (*1/*2), ADH1B (*2/*2); ALDH2 (*1/*2); ADH1B (*/1*1), ADH1B (*1/*2),
 ADH1B (*2/*2); ALDH2 (*2/*2). Group 3 Caucasian: ADH1B (*/1*1); ALDH2 (*1/*1)

 -  Part A: Japanese or Caucasian Male between 20 and 65 years of age inclusive, at the
 time of signing the informed consent. Part B: Males or Females between 18 and 55 years
 of age inclusive, at the time of signing the consent.

 -  Male subjects with female partners of child-bearing potential must agree to use one of
 the contraception methods listed in the protocol. This criterion must be followed from
 the time of the first dose of study medication until 21 days after the final dose.

 -  A female is eligible to enter and participate in this study if she is of
 non-childbearing potential (i.e., physiologically incapable of becoming pregnant),
 including any female who: Has had a hysterectomy or Has had a bilateral oophorectomy
 (removal of the ovaries) or Has had a bilateral tubal ligation or Is post-menopausal
 (a demonstration of total cessation of menses for ≥ 1 year from the date of screening
 visit). A follicle stimulating hormone (FSH) level will be performed to confirm a
 post-menopausal state. For this study FSH levels ≥ 40mIU/mL are consistent with
 menopause. If a subject is on estrogen replacement and menopausal status is
 questionable, estrogen replacement should be discontinued for 2 weeks and then the
 subject rescreened, as estrogen replacement can suppress FSH.

 -  Part A: For Japanese subjects: Body weight ≥ 50 kg and BMI within the range 18.5 -
 29.0 kg/m2 (inclusive). For Caucasian subjects: Body weight ≥ 50 kg and BMI within the
 range 19 - 32 kg/m2 (inclusive). Part B: Body weight ≥ 50 kg for males and 45 kg for
 females and BMI within the range 18.5- 31.0 kg/m2 (inclusive).

 -  Capable of giving written informed consent, which includes compliance with the
 requirements and restrictions listed in the consent form.

 -  QTc, QTcB or QTcF < 450 msec; or QTc < 480 msec in subjects with Bundle Branch Block.

 Exclusion Criteria:

 -  As a result of the medical interview, physical examination, or screening
 investigations, the Investigator considers the subject unfit for the study.

 -  History of bleeding or clotting disorders including disseminated intravascular
 coagulation, hemophilia Henoch-Schönlein purpura (allergic purpura), hereditary
 hemorrhagic telangiectasia, thrombocytopenia, thrombophilia or Von Willebrand's
 disease.

 -  A positive pre-study Hepatitis B surface antigen or positive Hepatitis C antibody
 result within 3 months of screening.

 -  A positive test for HIV antibody.

 -  The subject has participated in a clinical trial and has received an investigational
 product within the following time period prior to the first dosing day in the current
 study: 30 days, 5 half-lives or twice the duration of the biological effect of the
 investigational product (whichever is longer).

 -  Exposure to more than four new chemical entities within 12 months prior to the first
 dosing day.

 -  Use of prescription or non-prescription drugs, including vitamins, herbal and dietary
 supplements (including St John's Wort) within 7 days (or 14 days if the drug is a
 potential enzyme inducer) or 5 half-lives (whichever is longer) prior to the first
 dose of study medication, unless in the opinion of the Investigator and GSK Medical
 Monitor the medication will not interfere with the study procedures or compromise
 subject safety.

 -  Current or chronic history of liver disease, or known hepatic or biliary abnormalities
 (with the exception of Gilbert's syndrome or asymptomatic gallstones).

 -  A positive pre-study drug/alcohol screen.

 -  History of regular alcohol consumption within 6 months of the study defined as: an
 average weekly intake of >14 drinks for males or >7 drinks for females. One drink is
 equivalent to 12 g of alcohol: 12 ounces (360 ml) of beer, 5 ounces (150 ml) of wine
 or 1.5 ounces (45 ml) of 80 proof distilled spirits.

 -  History of sensitivity to any of the study medications, or components thereof or a
 history of drug or other allergy that, in the opinion of the investigator or GSK
 Medical Monitor, contraindicates their participation.

 -  Where participation in the study would result in donation of blood or blood products
 in excess of 500 mL within a 56 day period.

 -  Part A: Female subjects. Part B: Female subjects of child-bearing potential.
 Unwillingness or inability to follow the procedures outlined in the protocol.

 -  Subject is mentally or legally incapacitated.

 -  History of sensitivity to heparin or heparin-induced thrombocytopenia.

 -  Unwilling to abstain from alcohol for at least 7 days prior to dose until 21 days
 after the final dose of study medication.

 -  Part A: Subjects with a smoking history of >10 cigarettes per day in the last 3
 months. Part B: History or regular use of tobacco- or nicotine-containing products
 within 6 months prior to screening.

 -  Subjects with a creatinine clearance < 80 mL/min as calculated by the Cockcroft- Gault
 equation.[Cockcroft, 1976] CLcr (mL/min) = (140 - age) * Wt / (72 * Scr) (times 0.85
 if female) where age is in years, weight (Wt) is in kg, and serum creatinine (Scr) is
 in units of mg/dL.

 -  Consumption of red wine, seville oranges, grapefruit or grapefruit juice and pomelos,
 exotic citrus fruits, grapefruit hybrids or fruit juices from 7 days prior to the
 first dose of study medication.

 -  The subject's systolic blood pressure is outside the range of 90-140mmHg, or diastolic
 blood pressure is outside the range of 45-90mmHg or heart rate is outside the range of
 50-100bpm for female subjects or 45-100 bpm for male subjects.

 -  A recent history of symptomatic orthostatic hypotension

 -  History of clinically significant cardiovascular disease including: Exclusion criteria
 for screening ECG (a single repeat is allowed for eligibility determination): Heart
 rate (males) <45 and >100 bpm; heart rate (females) <50 and >100 bpm. PR interval <120
 and >220 msec. QRS duration <70 and >120 msec. QTc interval (Bazett) >450 msec (> 480
 msec for BBB); Evidence of previous myocardial infarction (pathologic Q waves, S-T
 segment changes (except early repolarization); History/evidence of symptomatic
 arrhythmia, angina/ischemia, coronary artery bypass grafting (CABG) surgery or
 percutaneous transluminal coronary angioplasty (PCTA) or any clinically significant
 cardiac disease; Any conduction abnormality (including but not specific to left or
 right complete bundle branch block, AV block [2nd degree (type II) or higher], Wolf
 Parkinson White [WPW] syndrome); Sinus pauses > 3 seconds; Any significant arrhythmia
 which, in the opinion of the principal Investigator and GSK Medical Monitor, will
 interfere with the safety for the individual subject.Non-sustained (≥3 consecutive
 ventricular ectopic beats) or sustained ventricular tachycardia.

 -  Subject who may be compromised by a major drop in hemoglobin and is unwilling to
 receive a blood transfusion if clinically indicated.