Assessment Of Vitamin D Role In The Pathogenesis Of Asthma In Vitamin D Resistent Patients
Assessment Of Vitamin D Role In The Pathogenesis Of Asthma In Vitamin D Resistent Patients
Research Title: Evaluation of airway reactivity, allergy and inflammatory mediators in
 exhaled breath condensate in vitamin D resistant rickets.

 Aim: To assess the effect of absence of vitamin D receptors on airway reactivity, allergy and
 inflammatory mediators in exhaled breath condensate in a prospective study evaluating
 patients with vitamin D resistant rickets and in healthy controls.

 Sample size: 40 participants in the two groups. Primary end point: Airway reactivity as
 assessed by methacholine challenge test.

 Secondary outcome parameters: All other parameters are the secondary end points.
Research Title: Evaluation of airway reactivity, allergy and inflammatory mediators in
 exhaled breath condensate in vitamin D resistant rickets.

 Introduction: Vitamin D seems to play a role in allergic and asthmatic reactions. Vitamin D
 resistant rickets patients lack vitamin D receptors and cannot absorb vitamin D.

 Aim: To assess the effect of absence of vitamin D receptors on airway reactivity, allergy and
 inflammatory mediators in exhaled breath condensate.

 Design: Prospective study evaluating these parameters in patients with vitamin D resistant
 rickets and in healthy controls.

 Participant selection: The study group will consist of vitamin D resistant rickets patients
 followed and treated at the Pediatric Endocrinology Department at our hospital. The study
 group will be compared to an age- and sex-matched healthy control group that had a negative
 methacholine challenge test.

 Sample size: 40 participants in the two groups. Intervention: Each subject will undergo
 evaluation including a respiratory questionnaire, pulmonary function tests, methacholine
 challenge test with determination of PC20, exhaled nitric oxide (eNO), and exhaled breath
 condensate (EBC). Venous blood will be analyzed for complete blood count + eosinophils, IGE
 levels, and Vitamin D levels. Prick skin test for inhaled allergens will be performed. All
 measurements will be evaluated in a single 3 hour visit, with no follow up study visits.

 Primary end point: Airway reactivity as assessed by methacholine challenge test.

 Secondary outcome parameters: All other parameters are the secondary end points.
Inclusion Criteria:

 -  Vitamin D Resistent Rickets

 -  Age between 3 - 40 years

 Exclusion Criteria:

 -  Any Chronic Lung Disease

 -  Febrile Illness in last 2 weeks

 -  Inhaled Corticosteroids over the past 2 weeks

 -  Bronchodilators over the past 24 hours

 -  Participation in any other clinical studies over the past 4 weeks