Studying Biomarkers in Cell Samples From Patients With Acute Myeloid Leukemia
Studying Biomarkers in Cell Samples From Patients With Acute Myeloid Leukemia
RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help
 doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.

 PURPOSE: This research study is looking at biomarkers in cell samples from patients with
 acute myeloid leukemia.
OBJECTIVES:

 -  To determine the significance of regulation of microRNA-34a and microRNA-194 by C/EBPα
 during granulopoiesis in patients with acute myeloid leukemia with CEBPA mutations.

 -  To determine whether overexpression of micro RNA-34a and microRNA-194 in myeloid
 progenitors with C/EBPα mutations leads to granulocytic differentiation.

 -  To examine how microRNA-34a and microRNA-194 downregulate E2F3 during granulopoiesis.

 -  To determine the role of microRNA-34a and microRNA-194 in myeloid cell proliferation.

 OUTLINE: Cryopreserved cell samples are collected for laboratory analysis, including mutation
 analysis, gene expression analysis, RNA analysis, and fluorescence-activated cell sorting
 (FACS) analysis.
DISEASE CHARACTERISTICS:

 -  Diagnosis of acute myeloid leukemia with CEBPA mutations

 PATIENT CHARACTERISTICS:

 -  Not specified

 PRIOR CONCURRENT THERAPY:

 -  Not specified