Study of Panobinostat (LBH589) in Patients With Sickle Cell Disease
Study of Panobinostat (LBH589) in Patients With Sickle Cell Disease
The goal of this clinical research study is to find out about the safety and effects of a
 drug called panobinostat when given to adults with sickle cell disease. Panobinostat is a pan
 histone deacetylase (HDAC) inhibitor. HDAC inhibitors have been shown to significantly
 increase hemoglobin F induction, which is well documented to improve outcomes in sickle cell
 disease. HDAC inhibitors are also known to potently inhibit cell-specific inflammation, which
 is a primary contributor to the debilitating effects of sickle cell disease. Given the
 relevance of these mechanisms of action in SCD, panobinostat may prove to contribute
 significantly to the management of SCD patients, a population in critical need of further
 effective treatment options.
This is a one-arm, open-label, Phase I, dose-escalation study of Panobinostat administered
 via different dosing schedules. In each schedule, this study is designed to determine the MTD
 (maximum tolerated dose) and DLT (dose limiting toxicities) of panobinostat as a single
 agent, and to characterize the safety and tolerability of panobinostat in adult patients with
 sickle cell disease who have failed to respond to hydroxyurea therapy (clinically or
 hematologically) or are intolerant of or refuse hydroxyurea therapy. The study consists of a
 Screening Phase, Treatment Phase, and Post-Treatment Follow-up.

 Screening Phase:

 Subjects will be screened for eligibility within 28 days of baseline visit (Day 1). Screening
 assessments will include informed consent, physical exam, vital signs (height, weight, blood
 pressure, heart rate, height, weight, and respirations), review of medical history, review of
 concomitant medications, 12-lead electrocardiogram (ECG), echocardiogram, and laboratory
 assessments. Screening laboratory assessments will include the following: CBC with
 differential, reticulocytes, complete chemistry panel, LDH, serum ferritin, thyroid function
 testing, Hb F percentage, F-cells, viral serology, urine albumin/creatinine ratio, and serum
 pregnancy in all females of childbearing potential. Approximately 15 ml (1 tablespoon) of
 blood will be collected at the screening visit.

 Treatment Phase:

 The Treatment Phase is twelve weeks in duration. Each subject will be assigned to a specified
 dose level and dosing schedule and will remain with the assigned regimen, if tolerated,
 throughout the twelve-week period. Patients at a given dosing regimen must complete at least
 4 weeks of treatment before treatment decisions are made for subsequent participants.
 Regardless of specific dosing assignment, all subjects will take study drug thrice weekly
 (Monday, Wednesday, and Friday) throughout the duration of the 12-week treatment period.

 The first three patients to enroll in the study will be assigned to a dose level of 15 mg
 panobinostat, with continuous thrice weekly treatment for the entire 12-week treatment
 period. Based on how well this regimen is tolerated, the next group of three subjects will
 either be assigned the same regimen or a reduced, intermittent schedule of three weeks on
 treatment followed by one week off.

 Treatment decisions will continue accordingly, after each group of three subjects is treated
 with a given regimen. Six patients must be treated safely at a given dosing regimen before
 enrollment can advance to the next, higher level. Once the Maximum Tolerated Dose is
 determined, all subsequent patients will be enrolled to that regimen, with a total of up to
 18 subjects enrolled. The four dosing regimens to be explored are:

 1. 15 mg MWF 3 weeks on, 1 week off (only needed if starting regimen requires dose
 de-escalation)

 2. 15 mg MWF every week (starting dose)

 3. 20 mg MWF 3 weeks on, 1 week off

 4. 20 mg MWF every week During the Treatment Phase, safety and efficacy assessments will be
 performed at specified times, and will include: physical examination, vital signs
 (weight, blood pressure, heart rate, height, weight, and respirations), adverse events
 assessment, ECG, quality of life questionnaire (ASCQMe), and laboratory assessments.
 Treatment Phase lab assessments are to be performed at specified visits and include CBC
 with differential, reticulocytes, complete chemistry panel, LDH, serum ferritin, Hb F
 percentage, F-cells, thyroid function testing, urine albumin/creatinine ratio, and urine
 pregnancy test for women of childbearing potential. Approximately 15 ml (3 teaspoons) of
 blood will be collected at each treatment visit. Every four weeks, an additional 5 ml of
 blood will be collected for inflammatory markers and protein biomarkers, for a total of
 20 ml. On Day 1 (pre-treatment) and Day 85 (post-treatment), when additional samples are
 collected for genetic/ mechanistic studies, total volume collected is 25 ml.

 Study drug will initially be dispensed at the baseline visit and every four weeks thereafter.
 Study drug accountability should be assessed at every visit. All baseline assessments will be
 completed prior to the first dose of study drug.

 Follow-Up Phase:

 A follow-up visit will be performed 4 weeks after end of treatment. Follow-up assessments
 will include: physical examination, vital signs, adverse events assessment, ECG, and
 laboratory assessments (CBC with differential, reticulocytes, complete chemistry panel, LDH,
 Hb F percentage, F-cells, and inflammatory markers). Approximately 15 ml (3 teaspoons) of
 blood will be collected at the follow-up visit.
Inclusion Criteria

 1. Male or female patients ages ≥ 18 years

 2. Confirmed diagnosis of homozygous SS or S-β0Thalassemia

 3. Intolerance to hydroxyurea therapy, refusal of hydroxyurea therapy, or failure to
 respond (refractoriness) to hydroxyurea therapy, either clinically or hematologically.

 4. Clinically significant sickle cell disease as defined by:

 1. At least two hospitalizations over the past twelve months for any complication of
 sickle cell disease; or

 2. At least three pain crises over the past twelve months that last four or more
 hours and require a visit to a medical facility for treatment with oral or
 parenteral narcotics; or

 3. History of recurrent leg ulcers; or

 4. History of Acute Chest Syndrome within the past five years; or

 5. History of priapism requiring medical intervention within the past two years; or

 6. History of stroke (but not currently on a chronic blood transfusion regimen).

 5. Ability to provide written informed consent obtained prior to participation in the
 study and any related procedures being performed.

 6. Clinically euthyroid. Note: Patients are permitted to receive thyroid hormone
 supplements to treat underlying hypothyroidism.

 Exclusion Criteria

 1. Use of agents that can induce Hb F within 60 days of Day 1 (i.e. hydroxyurea,
 butyrates, decitabine, 5-azacytidine, IMiDs®, or erythropoietin). Prior use of HDACi,
 including panobinostat, is not an exclusion criterion if discontinued > 60 days.

 2. Patients who have had a vaso-occlusive crisis within the past 2 weeks that required
 treatment with parenteral medication.

 3. Impairment of GI function or GI disease that may significantly alter the absorption of
 panobinostat (e.g., ulcerative diseases, uncontrolled nausea, vomiting, diarrhea,
 malabsorption syndrome, or small bowel resection)

 4. Patients on a chronic transfusion regimen, or any patient who has Hb A% > 20% from a
 recent transfusion

 5. Any of the following laboratory abnormalities derived from the screening visit:

 -  Absolute neutrophil count (ANC) < 1.5 x 109/L

 -  Hemoglobin < 6 g/dl

 -  Platelets < 100x 109/L

 -  Serum creatinine >1.5 x Upper limits of normal (ULN)

 -  AST and ALT > 2.5 x ULN

 -  Serum total bilirubin > 10 mg/dL

 -  Serum direct bilirubin > 1 mg/dL

 -  Albumin <3.0 g/dl

 -  Serum potassium < Lower limits of normal (LLN)

 -  Total serum calcium [corrected for serum albumin] or ionized calcium <LLN

 -  Serum magnesium < LLN

 -  Serum phosphorus < LLN

 6. Known impaired cardiac function or clinically significant cardiac diseases, including
 any one of the following:

 -  Left ventricular ejection fraction (LVEF) < lower limit of the institutional
 normal as determined by screening echocardiogram

 -  Complete left bundle branch block

 -  Obligate use of a cardiac pacemaker

 -  Congenital long QT syndrome

 -  History or presence of ventricular tachyarrhythmia

 -  Presence of unstable atrial fibrillation (ventricular response > 100 bpm).
 Patients with stable atrial fibrillation are eligible, provided they do not meet
 any of the other cardiac exclusion criteria.

 -  Clinically significant resting bradycardia (< 50 bpm)

 -  QTc > 470 msec on screening ECG

 -  Right bundle branch block + left anterior hemiblock (bifasicular block)

 -  Angina pectoris 3 months prior to starting study drug

 -  Acute MI 3 months prior to starting study drug

 -  Other clinically significant heart disease (e.g., CHF, uncontrolled hypertension,
 history of labile hypertension, or history of poor compliance with an
 antihypertensive regimen)

 7. Other concurrent severe and/or uncontrolled medical conditions (e.g., uncontrolled
 diabetes, active or uncontrolled infection, chronic obstructive or chronic restrictive
 pulmonary disease) that could cause unacceptable safety risks or compromise compliance
 with the protocol

 8. Patients who are currently receiving treatment with any study drug or have been on any
 study medications within the past 60 days.

 9. Patients who have undergone major surgery 2 weeks prior to starting study drug or who
 have not recovered from side effects of such therapy.

 10. Women of child-bearing potential (WCBP) who are pregnant or breast feeding or who do
 not agree to use two methods of birth control, including a barrier method, if they are
 sexually active. WCBP, defined as sexually mature women who have not undergone a
 hysterectomy or who have not been naturally postmenopausal for at least 12 consecutive
 months (i.e., who has had menses any time in the preceding 12 consecutive months),
 must have a negative serum pregnancy test at screening and negative urine pregnancy
 test within 72 hours prior to starting study treatment. In addition, all sexually
 active WCBP must agree to use double method of contraception (oral, injectable, or
 implantable hormonal contraceptive; tubal ligation; intra-uterine device; barrier
 contraceptive with spermicide; or vasectomized partner) during the study and 3 months
 after the end of treatment. One of these methods of contraception must be a barrier
 method.

 11. Male patients whose sexual partners are WCBP not using a double method of
 contraception during and 3 months after the end of treatment. Males must agree to use
 a condom during any sexual contact with WCBP during study drug treatment, during dose
 interruptions, and for 3 months after the end of treatment.

 12. Known diagnosis of HIV infection, Hepatitis B; or acute/chronic, active Hepatitis C

 13. Patients with a prior malignancy with in the last 5 years (except for basal or
 squamous cell carcinoma, or in situ cancer of the cervix)

 14. Patients with any significant history of non-compliance to medical regimens or
 unwilling or unable to comply with the instructions given to him/her by the study
 staff.

 15. Patients who are currently receiving treatment with certain prohibited medications and
 cannot either discontinue this treatment or switch to a different medication prior to
 study enrollment.