Study by Metagenomics and Culturomicsapproaches of Bacterial and Viral Flora of Diabetic Foot Infection
Study by Metagenomics and Culturomicsapproaches of Bacterial and Viral Flora of Diabetic Foot Infection
This work also highlights the presence of an unknown virus double-stranded DNA. In this
 project we propose 1) incorporating a bank of 150 samples from patients with diabetic foot
 infection in grades 2-4; 2) to study the microbial flora of a selection of 50 diabetic feet
 previously untreated with antibiotics for bacterial metagenomics, viral metagenomics and a
 minimum of 10 per culturomique; 3) to evaluate the use of targeted quantitative PCR on the
 most frequent new species frequency in the disease and incidentally get a sense of their role
 in the evolution and prognosis of the disease, including failures of and targeted antibiotic
 therapy on all 150 samples.
Patients will be included by the infectious and tropical diseases and diabetes services the
 Nîmes Teaching Hospital which have a large active file and proven expertise internationally
 in this pathology. The usual agents of infection will be searched by standard bacteriological
 techniques as part of the microbiological documentation of such cases and will be performed
 by the microbiology laboratory of CHU Nîmes which will freeze the rest of the sample to -80 °
 C the will send it to the Marseille Microbiology Federation for realization of
 metagenomics-culturomics
Inclusion Criteria:

 -  Patients were taken care by the services(departments) of Diabetology or Infectious
 diseases of the CHU(TEACHING HOSPITAL) of Nîmes, were hospitalized or were seen in
 consultation and presenting a wound chronicle of the foot and/or the ankle of Rank 2 -
 4 (Classification of the IWGDF) [IWGDF, Lipsky Clin Infect Dis on 2004], inaugural or
 récidivante.

 Exclusion Criteria:

 -  The patient participates in another study

 -  The patient is in period of exclusion determined by a previous study

 -  The patient is under protection(saving) of justice, under guardianship or under
 guardianship

 -  The patient refuses to sign the consent

 -  It turns out impossible to give about the lit(enlightened) information

 -  The patient is pregnant, parturiente, or she breast-feeds

 -  The patients having no wound

 -  The patients diabetics having a wound chronicles of Rank 1 (Classification of the
 IWGDF) [IWGDF, Lipsky Clin Infect Dis on 2004]