Comparison of Bone Morphogenetic Protein-4 (BMP-4) and 7 Levels Between Control and Polycystic Ovarian Syndrome (PCOS) Patients.
Comparison of Bone Morphogenetic Protein-4 (BMP-4) and 7 Levels Between Control and Polycystic Ovarian Syndrome (PCOS) Patients.
The investigators aim to compare the levels of bone morphogenetic protein-4 and -7 (BMP-4 and
 7) in blood, follicular fluid and ovarian organ culture supernatant obtained from healthy
 subjects and polycystic ovarian syndrome (PCOS) patients.
Female patients at ages 20-35 diagnosed with polycystic ovarian syndrome and age-matched
 healthy control subjects will be enrolled in this study.

 Blood samples will be obtained at early follicular phase during doctor's visit from PCOS
 patients and control subjects.If the patient is amenorrheic at the time of visit, both random
 blood samples and another sample at early follicular phase after induction of withdrawal
 bleeding with progesterone.

 Follicular fluids will be retrieved during oocyte pick-up procedure in patients undergoing
 assisted reproduction for PCOS and control IVF patients whose infertility is not PCO related.

 Samples from ovaries will be collected at operation from PCOS patients and control subjects
 if they already undergo any gynecologic laparotomi operations.

 Collected samples will be cultured for 7 days and BMP-4 and -7 levels will be measured in the
 culture fluid of the samples using ELISA method.
Inclusion Criteria:

 -  Healthy subjects and PCOS patients at ages 20-35 will be recruited for the study.

 Exclusion Criteria:

 -  Those with any metabolic or endocrine problems other than PCOS will be excluded from
 the study.