A Study to Determine Safety, Feasibility and Efficacy of JCAR015 in Adult Subjects With B-Cell Acute Lymphoblastic Leukemia
A Study to Determine Safety, Feasibility and Efficacy of JCAR015 in Adult Subjects With B-Cell Acute Lymphoblastic Leukemia
This is a single-arm, multi-center, open-label, Phase 2 study to determine the efficacy and
 safety of JCAR015 in adult subjects with B-cell ALL. The study is divided into two sequential
 parts, Part A and Part B; subjects will be screened and will provide informed consent before
 initiating any study procedures in Part A of the study.
Inclusion Criteria:

 1. Age ≥18 years at the time of signing the informed consent form

 2. Subject must understand and voluntarily sign an Informed consent form (ICF) prior to
 any study-related assessments/procedures being conducted

 3. Subject is willing and able to adhere to the study visit schedule and other protocol
 requirements

 4. Diagnosis of B-cell Acute Lymphoblastic Leukemia (ALL)

 5. Evidence of CD19 expression via flow cytometry (peripheral blood or bone marrow) or
 immunohistochemistry (bone marrow biopsy)

 6. Eastern Cooperative Oncology Group (ECOG) performance status ≤ 2

 7. No contraindications to cyclophosphamide. This includes subjects with:

 -  hypersensitivity to cyclophosphamide, any of its metabolites

 -  acute infections

 -  bone marrow aplasia or bone marrow depression prior to treatment

 -  urinary tract infection

 -  acute urothelial toxicity from cytotoxic chemotherapy or radiation therapy

 -  urinary outflow obstruction

 -  obstruction

 8. Adequate organ function, defined as:

 a. Serum creatinine ≤ 1.5 × age-adjusted upper limit of normal (ULN) OR calculated
 creatinine clearance (Cockcroft and Gault) > 30 mL/min/1.73 m2 b. Alanine
 aminotransferase (ALT) ≤ 5 × ULN and direct bilirubin < 2.0 mg/dL (or < 3.0 mg/dL for
 subjects with leukemic infiltration of the liver) c. Adequate pulmonary function,
 defined as ≤ Grade 1 dyspnea and oxygen saturation (SaO2) ≥ 92% on room air d.
 Adequate cardiac function, defined as left ventricular ejection fraction (LVEF) ≥ 40%
 as assessed by echocardiogram (ECHO) or multiple uptake gated acquisition (MUGA)
 performed within 1 month of signing the informed consent form

 9. Adequate central or peripheral vascular access for leukapheresis procedure. For
 subjects requiring central venous catheter (CVC) placement, a surgical consultation
 indicating subject eligibility for CVC placement is sufficient for enrollment.

 10. Subjects must agree to not donate blood, organs, sperm or semen, and egg cells for
 usage in other individuals without recipient knowledge about exposure to a genetically
 modified organ by the donor and having been informed about the potential risks
 associated with it at any point after receiving JCAR015 infusion.

 11. Females of childbearing potential (FCBP1) must:

 a. Have two negative pregnancy tests as verified by the Investigator (one negative
 serum beta human chorionic gonadotropin (ß-hCG) pregnancy test result within 7 days
 prior to the first dose of cytoreductive chemotherapy (Part A screening evaluation),
 and one negative serum or urine pregnancy test at the Part B screening evaluation
 prior to first JCAR015 infusion). She must agree to have another pregnancy test 90
 days post final JCAR015 dose. This applies even if the subject practices true
 abstinence2 from heterosexual contact.

 b. Either commit to true abstinence2 from heterosexual contact (which must be reviewed
 on a monthly basis and source documented) or agree to use, and be able to comply with,
 effective contraception without interruption. Contraception methods must include 1
 highly effective and 1 additional effective (barrier) method of contraception from at
 least 28 days prior to JCAR015 initial infusion and during the study therapy including
 dose interruptions. Cessation of contraception after this point should be discussed
 with a responsible physician. Note: Highly effective methods are defined as those that
 result in a low failure rate (ie, less than 1% per year) when used consistently and
 correctly. The following are examples of highly effective and additional effective
 methods of contraception:

 - Intrauterine device (IUD)

 - Hormonal (birth control pill, injections, implants)

 - Tubal ligation

 - Partner's vasectomy

 - Male Condom (additional effective method)

 - Diaphragm (additional effective method)

 - Cervical Cap (additional effective method) c. Agree to abstain from breastfeeding
 during study participation and for at least 90 days after the last dose of JCAR015.

 d. Agree to use highly effective methods of contraception during the entire study
 period (Part A through 12 months after the final JCAR015 infusion).

 12. Males who have partners of childbearing potential must agree to use an effective
 barrier contraceptive method during the entire study period (Part A through 12 months
 after the final JCAR015 infusion).

 Additional Cohort Specific Inclusion Criteria

 Inclusion Criteria (Cohorts 1-4) 1. Relapsed or refractory disease, defined as:

 1. First bone marrow relapse (prior Complete response (CR) duration < 18 months) or any
 subsequent bone marrow relapse from Complete response (CR), OR

 2. Any bone marrow relapse after allogeneic hematopoietic stem cell transplant (HSCT);
 subjects must be at least 2 months from HSCT at the time of signing the informed
 consent form and off immunosuppressant medication for at least 1 month at the time of
 signing the informed consent form (with the exception of low-dose steroids (≤ 20 mg
 prednisone or equivalent), and have no Graft versus host disease (GVHD), OR

 3. Refractory Acute Lymphoblastic Leukemia (ALL), defined by not having achieved a
 Complete response (CR) or Complete response with incomplete peripheral blood count
 recovery (CRi) after two attempts at remission induction using standard chemotherapy
 regimens, OR

 4. Philadelphia chromosome positive (Ph+) B-cell ALL if subjects are intolerant to, or
 ineligible for, Tyrosine kinase inhibitor (TKI) therapy, or have progressed after at
 least one line of TKI therapy.

 2. Bone marrow with morphological evidence of disease (≥ 5% blasts by morphology).

 Inclusion Criteria [Ph(+) (Cohort 3)] All subjects must meet Inclusion criteria
 described for Cohort 1.

 Inclusion Criteria (Cohort 4 only) 1. Eligible for Cohorts 1-3 but investigator has
 decided to administer blinatumomab as standard ALL therapy rather than other
 alternatives listed after leukapheresis but prior to part B of therapy Inclusion
 Criteria (MRD Positive+ [Cohort 5])

 Inclusion criteria as for Cohorts 1 and 2, with the exception of:

 1. Bone marrow without morphological evidence of disease characterized as ≤ 5% blasts
 by morphology, but with MRD detected by flow cytometry, or qPCR at a frequency of ≥ 1
 x 10-4 following standard of care induction and consolidation therapy in CR1 or in any
 subsequent CR

 -  For subjects in whom MRD has been followed only by flow cytometry, a nucleic acid
 sample of the leukemia blasts of sufficient quality must be available to generate
 realtime quantitative PCR (RQ-PCR) primers.

 4.2.2. Inclusion Criteria (Part B)

 1. Completion of Part A and successful generation of a JCAR015 cell product 2. Results
 from bone marrow examination following Part A:

 1. Group 1: Morphological evidence of disease (≥ 5% blasts by morphology)

 2. Group 2: Morphologic complete remission (bone marrow with < 5% blasts) with or without
 blood count recovery (CR or CRi) or a hypoplastic, aplastic, or "recovery" marrow at
 Day 42 of Part A 3. Eastern Cooperative Oncology Group (ECOG) performance status
 between 0 and 2 4. Adequate organ function, defined as:

 1. Serum creatinine ≤ 1.5 × age-adjusted ULN OR calculated creatinine clearance

 2. (Cockcroft and Gault) > 30 mL/min/1.73 m2

 3. ALT ≤ 5 × ULN (or ≤ 8 × ULN for subjects with leukemic infiltration of the liver) and
 direct bilirubin < 2.0 mg/dL (or < 3.0 mg/dL for subjects with leukemic infiltration
 of the liver)

 4. Adequate pulmonary function, defined as ≤ Grade 1 dyspnea and SaO2 ≥ 92% on room air
 5. Females of childbearing potential and males who have partners of childbearing
 potential follow the same inclusion criteria as Part A.

 Exclusion Criteria:

 1. Isolated extramedullary disease relapse

 2. Concomitant genetic syndrome such as Fanconi anemia, Kostmann syndrome, Shwachman
 syndrome, or any other known bone marrow failure syndrome

 3. Burkitt's lymphoma/leukemia or chronic myelogenous leukemia (CML) lymphoid blast
 crisis (p210 BCR-ABL+)

 4. Prior malignancy, unless treated with curative intent and with no evidence of active
 disease present for > 5 years before signing the informed consent form, with the
 following exceptions:

 a. Subjects with Stage I breast cancer that has been completely and successfully
 treated, requiring no therapy or only anti-hormonal therapy b. Subjects with T1N0M0 or
 T2N0M0 colorectal cancer who have been completely and successfully resected and who
 are disease-free for > 2 years prior to screening c. Subjects with indolent prostate
 cancer, defined as clinical stage T1 or T2a, Gleason score ≤ 6, and prostate-specific
 antigen (PSA) < 10 ng/mL, requiring no therapy or only anti- hormonal therapy d.
 Subjects with a history of basal cell or squamous cell carcinoma of the skin, or
 carcinoma in situ of the cervix, fully resected, and with no evidence of active
 disease

 5. Treatment with any prior gene therapy product

 6. Active hepatitis B, active hepatitis C, or any human immunodeficiency virus (HIV)
 infection at the time of signing the informed consent form

 7. Systemic fungal, bacterial, viral, or other infection that is not controlled (defined
 as exhibiting ongoing signs/symptoms related to the infection and without improvement,
 despite appropriate antibiotics or other treatment) at the time of signing the
 informed consent form

 8. Presence of Grade II-IV (Glucksberg) or B-D (IBMTR) acute or extensive chronic GVHD at
 the time of signing the informed consent form

 9. Active C S involvement by malignancy, defined as CNS-3 per NCCN guidelines. Subjects
 with a history of Central nervous system (CNS) disease that has been effectively
 treated (defined as one documented negative CSF evaluation within 1 month prior to
 signing the informed consent form) will be eligible

 10. History of any one of the following cardiovascular conditions within the past 6 months
 of signing the informed consent form: Class III or IV heart failure as defined by the
 New York Heart Association (NYHA), cardiac angioplasty or stenting, myocardial
 infarction, unstable angina, or other clinically significant cardiac disease

 11. History or presence of clinically relevant CNS pathology such as epilepsy, generalized
 seizure disorder, paresis, aphasia, stroke, severe brain injuries, dementia,
 Parkinson's disease, cerebellar disease, organic brain syndrome, or psychosis

 12. Participation in an investigational research study using an investigational agent
 within 30 days of signing the informed consent form, with the exception of
 investigational antiinfective agents (eg, antibacterial, antifungal, antiviral)

 13. History of treatment with a murine-derived biological product (unless subject has been
 shown to be negative for human anti-mouse antibodies [HAMA] prior to or during
 screening). Prior use of blinatumomab is permitted (provided there is evidence of CD19
 expression per Part A Inclusion Criterion #4). Chimeric biological products (eg,
 rituximab) are not considered murine for the purpose of this protocol.

 14. Pregnant or nursing (lactating) women

 15. Use of prohibited medications:

 a. Steroids: Therapeutic doses of corticosteroids (defined as > 20 mg/day prednisone
 or equivalent) are prohibited within 7 days prior to leukapheresis. Physiologic
 replacement dosing of steroids (≤ 12 mg/m2/day hydrocortisone or equivalent [≤ 3
 mg/m2/day prednisone or ≤ 0.45 mg/m2/day dexamethasone]) is allowed. Topical steroids
 and intrathecal steroids for CNS relapse prophylaxis are permitted.

 b. Allogeneic cellular therapy: Donor lymphocyte infusions (DLI) are prohibited within
 4 weeks prior to leukapheresis c. GVHD therapies: Any drug used for GVHD within 4
 weeks prior to leukapheresis, eg, calcineurin inhibitors, methotrexate or other
 chemotherapeutics, mycophenolate mofetil, rapamycin, thalidomide, immunosuppressive
 antibodies (such as anti-CD20 [rituximab], anti-TNFα, anti-IL-6, or anti-IL-6R) d.
 Chemotherapies: Salvage chemotherapy (eg, cytosine arabinoside > 100 mg/m2/day,
 anthracyclines, and cyclophosphamide) must be stopped at least 1 week prior to
 leukapheresis

 16. Treatment with alemtuzumab within 6 months prior to leukapheresis, or treatment with
 clofarabine or cladribine within 3 months prior to leukapheresis

 17. Uncontrolled medical, psychological, familial, sociological, or geographical
 conditions that do not permit compliance with the protocol, as judged by the
 Investigator; or subject unwillingness or inability to follow the procedures required
 in the protocol 4.3.2. Exclusion Criteria (Part B)

 Subjects must not meet any of the following criteria prior to Part B to be considered
 eligible:

 1. Systemic fungal, bacterial, viral, or other infection that is not controlled (defined
 as exhibiting ongoing signs/symptoms related to the infection and without improvement,
 despite appropriate antibiotics or other treatment)

 2. Active CNS involvement by malignancy, defined as CNS-3 per NCCN guidelines. Subjects
 with a history of CNS disease that has been effectively treated (defined as one
 documented negative CSF evaluation within 1 month prior to signing the informed
 consent form) will be eligible

 3. Presence of clinically relevant CNS pathology such as epilepsy, generalized seizure
 disorder, paresis, aphasia, stroke, severe brain injuries, dementia, Parkinson's
 disease, cerebellar disease, organic brain syndrome, or psychosis

 4. Presence of Grade II-IV (Glucksberg) or B-D (IBMTR) acute or extensive chronic GVHD

 5. Use of prohibited medications

 6. Female subjects that have a positive serum or urine pregnancy test, or are pregnant