Antibacterial Activity of Urea Against Ocular Bacteria
Antibacterial Activity of Urea Against Ocular Bacteria
Antibacterial properties of urea was tested against 35 isolates from ocular infections.
In vitro susceptibility testing was performed with commercially available antibiotic discs,
 homemade urea discs saturated with urea solution at a concentration of 96 mmol. The weight of
 urea in one drop = 0.37 mg, in one ml = 10.34 mg (5 microliter/ disc) discs and combined
 discs (antibiotic discs to which urea solution was added). Combined discs provided better in
 vitro susceptibility results than antibiotics alone.

 Scanning electron micrographs of bacterial isolates incubated overnight with urea solution
 showed marked disruption of bacterial aggregative patterns and irregularities of their cell
 walls.

 Western blotting showed marked reduction in total proteins in bacterial isolates incubated
 overnight with urea solution. These findings may explain the possible mechanisms for
 antibacterial activity of urea solution as a result of protein denaturation.
Inclusion Criteria:

 -  ocular infection

 Exclusion Criteria:

 -  Local antibiotics treatment