Blastocyst Culture in a Time-lapse Incubator
Blastocyst Culture in a Time-lapse Incubator
Culture media used to support development of zygotes to the blastocyst stage is based on two
 basic philosophies: a single step medium "let-the-embryo-choose" or a sequential medium
 "back-to-nature".

 For single step media, there are two types of protocol can be used for the culture of human
 preimplantation embryos:

 1. Interrupted single medium culture, in which the medium is renewed on the Day 3 of embryo
 culture

 2. Uninterrupted single embryo culture, in which the same single medium is used throughout
 the 5-6 days of culture with no replenishment or replacement on Day 3.

 Investigators aim to compare the blastocyst development and clinical outcome between the two
 types of protocols of a single medium (with or without renewal on Day 3)
Inclusion Criteria:

 -  female partners were under the age of 39 years

 -  Intracytoplasmic sperm injection (ICSI) was performed using fresh ejaculates

 Exclusion Criteria:

 -  Day 3 embryo transfer cycles