Blood Flow and Pain Crises in People With Sickle Cell Disease
Blood Flow and Pain Crises in People With Sickle Cell Disease
Background:

 - Many people with sickle cell disease have repeated episodes of severe pain that lasts for
 days, requiring hospital care. These episodes, called pain crises, may be caused by changes
 in blood flow. Researchers want to study blood flow in people with sickle cell disease who
 are having a pain crisis and compare it with their blood flow after the pain crisis has
 resolved. They also want to compare these measurements against blood flow in healthy people
 who do not have sickle cell disease.

 Objectives:

 - To study whether changes in blood flow cause pain crises in people with sickle cell
 disease.

 Eligibility:

 -  Individuals at least 18 years of age who have sickle cell disease and are being treated
 for a pain crisis.

 -  Individuals at least 18 years of age who have sickle cell disease and are not
 experiencing a pain crisis.

 -  Healthy volunteers matched by age and gender with the participants who have sickle cell
 disease.

 Design:

 -  Participants will be screened with a physical exam and medical history. Blood and urine
 samples will be collected.

 -  Participants having a sickle cell pain crisis will have two visits, one during the
 crisis and one about 4 weeks after the crisis has resolved.

 -  Participants not having a sickle cell pain crisis will have one or two study visits.
 Blood samples will be collected during at least one of these visits.

 -  Healthy volunteers will have one or two study visits. Blood samples will be collected
 during at least one of these visits.

 -  During each visit for all participants, cameras and blood flow monitoring equipment will
 be used to measure blood flow in the forearm.

 sickle cell disease.
Severe recurrent pain is the most common cause of acute morbidity in sickle cell disease1.
 The underlying pathogenesis was initially thought to be ischemia from obstruction of
 capillary beds by stiffened red blood cells; however, there is evidence that other factors
 contribute to the pathogenesis of sickle cell pain crisis, such as inflammation and
 coagulation, ischemia-reperfusion injury, angiogenesis balance, and vasomotor tone processes
 that are regulated by endothelial nitric oxide.

 Recent clinical data suggest that subjects with sickle cell disease suffer from chronically
 impaired nitric oxide bioavailability. This has been attributed to increased consumption of
 nitric oxide by hemoglobin and reactive oxygen species, or decreased production of nitric
 oxide by endothelial cells; however the roles of nitric oxide bioavailability and endothelial
 dysfunction during acute pain crisis are controversial and incompletely understood. Although
 there have been several studies of endothelial function in steady state sickle cell disease,
 there has been no comprehensive study of endothelial function during pain crisis.

 In this study, our primary objective is to measure the reactive hyperemia index (a measure of
 the endothelial response to shear stress) in twenty sickle cell subjects during acute pain
 crisis and to compare it with the reactive hyperemia index measured after recovery from pain
 crisis. This will identify whether there are acute changes in endothelial cell function
 during sickle cell pain crisis. Our secondary objective is to compare the reactive hyperemia
 index of thirty-five sickle cell subjects in steady state versus the reactive hyperemia index
 of thirty-five healthy control subjects. This will identify whether there are chronic
 differences in endothelial function between sickle cell subjects and healthy control
 subjects.

 This study will determine if there are defects in endothelium-dependent vasodilation in
 response to shear stress during sickle cell pain crisis. Moreover, this study provides an
 opportunity to evaluate new physiologic biomarkers of sickle cell pain crisis based on
 measurements of blood flow, temperature, and oxygenation in the skin. These measurements may
 serve as clinical endpoints in future studies of disease pathogenesis or therapeutic
 interventions for sickle cell disease.
-  INCLUSION CRITERIA FOR SUBJECTS WITH SICKLE CELL DISEASE IN PAIN CRISIS:

 1. Age 18 years or older.

 2. Diagnosis of sickle cell anemia:

 1. Diagnosis of sickle cell disease (electrophoresis or HPLC documentation of
 hemoglobin SS, SC, S-beta-thalassemia or other hemoglobinopathies causing
 sickle cell disease is required).

 2. Acute onset pain crisis in a distribution typical for that subject, onset
 within the last 7 days and for which hospitalization and parenteral narcotic
 pain treatment are required.

 3. Ability to provide informed written consent.

 EXCLUSION CRITERIA FOR SUBJECTS WITH SICKLE CELL DISEASE IN PAIN CRISIS:

 1. Pregnancy.

 2. History of non-trivial injury, burns, surgery or skin ulcers on the arms.

 3. Carrier of drug resistant bacteria that normally requires isolation while visiting a
 hospital.

 4. Administration of any of the following drugs within the last 14 days:

 -  Phosphodiesterase-5 inhibitors (sildenafil, vardenafil, tadalafil)

 -  Endothelin-1 receptor blockers (bosentan, sitaxentan, ambrisentan, tezosentan)

 -  Nitric oxide donors (nitroglycerin, nitroprusside, nitrates)

 5. Ingestion of caffeine within the 12 hours before the start of the study appointment,
 or tobacco use within the 30 days before the study appointment.

 6. Diagnosis with any of the following chronic diseases or conditions:

 -  Uncontrolled high blood pressure (systolic blood pressure must not be greater
 than 160 mmHg or diastolic blood pressure greater than 90 mmHg)

 -  Uncontrolled high cholesterol (total cholesterol must not be greater than 240
 mg/dL)

 -  Uncontrolled diabetes (must not have both a documented history of diabetes and
 random blood glucose of greater than 200 mg/dL)

 -  Chronic kidney disease (serum creatinine must not be greater than 2 mg/dL)

 -  Coronary artery disease

 -  Peripheral vascular disease

 7. Received a blood transfusion within 7 days of the study procedure.

 SICKLE CELL DISEASE SUBJECTS IN STEADY STATE

 INCLUSION CRITERIA FOR SUBJECTS WITH SICKLE CELL DISEASE IN STEADY STATE:

 1. Age 18 years or older.

 2. Diagnosis of sickle cell anemia:

 a.Diagnosis of sickle cell disease (electrophoresis or HPLC documentation of
 hemoglobin SS, SC, S-beta-thalassemia or other hemoglobinopathies causing sickle cell
 disease is required).

 3. Ability to provide informed written consent.

 EXCLUSION CRITERIA FOR SUBJECTS WITH SICKLE CELL DISEASE IN STEADY STATE:

 1. Pregnancy.

 2. History of non-trivial trauma, burns, surgery or skin ulcers on the arms.

 3. Carrier of drug resistant bacteria that normally requires isolation while visiting a
 hospital.

 4. Experience of an acute pain crisis requiring intravenous (IV) narcotics and hospital
 admission within the last 14 days.

 5. Ingestion of caffeine within the 12 hours before the start of the study appointment,
 or tobacco use within the 30 days before the study appointment.

 6. Administration of any of the following drugs within the last 14 days:

 -  Phosphodiesterase-5 inhibitors (sildenafil, vardenafil, tadalafil)

 -  Endothelin-1 receptor blockers (bosentan, sitaxentan, ambrisentan, tezosentan)

 -  Nitric oxide donors (nitroglycerin, nitroprusside, nitrates)

 7. Diagnosis of any of the following chronic diseases or conditions: <TAB>

 -  Uncontrolled high blood pressure (systolic blood pressure must not be greater
 than 160 mmHg or diastolic blood pressure greater than 90 mmHg)

 -  Uncontrolled high cholesterol (total cholesterol must not be greater than 240
 mg/dL)

 -  Uncontrolled diabetes (must not have both a documented history of diabetes and
 random blood glucose of greater than 200 mg/dL)

 -  Chronic kidney disease (serum creatinine must not be greater than 2 mg/dL)

 -  Coronary artery disease

 -  Peripheral vascular disease

 8. Received a blood transfusion within 7 days of the study procedure.

 HEALTHY CONTROL SUBJECTS

 INCLUSION CRITERIA FOR HEALTHY CONTROL SUBJECTS

 1. Age 18 years or older.

 2. African, of African descent or Hispanic

 3. Ability to provide informed written consent.

 EXCLUSION CRITERIA FOR HEALTHY CONTROL SUBJECTS

 1. Pregnancy.

 2. History of non-trivial injury, burns, surgery or skin ulcers on the arms.

 3. Carrier of drug resistant bacteria that normally requires isolation while visiting a
 hospital.

 4. Administration of any of the following drugs within the last 14 days:

 -  Phosphodiesterase-5 inhibitors (sildenafil, vardenafil, tadalafil)

 -  Endothelin-1 receptor blockers (bosentan, sitaxentan, ambrisentan, tezosentan)

 -  Nitric oxide donors (nitroglycerin, nitroprusside, nitrates)

 5. Ingestion of caffeine in the 12 hours before the start of the study appointment, or
 used tobacco in the 30 days before the study appointment.

 6. Diagnosis with any of the following chronic diseases or conditions:

 -  Sickle cell disease <TAB>

 -  Uncontrolled high blood pressure (systolic blood pressure must not be greater
 than 160 mmHg or diastolic blood pressure greater than 90 mmHg)

 -  Uncontrolled high cholesterol (total cholesterol must not be greater than 240
 mg/dL)

 -  Uncontrolled diabetes (must not have both a documented history of diabetes and
 random blood glucose of greater than 200 mg/dL)

 -  Chronic kidney disease (serum creatinine must not be greater than 2 mg/dL)

 -  Coronary artery disease

 -  Peripheral vascular disease