Crucumin Effects on the Immune System in Osteoarthritis Patients
Crucumin Effects on the Immune System in Osteoarthritis Patients
In this study, the effects of crucumin on cellular and humoral immune system in patients with
 osteoarthritis will be investigated. Concentration of CXCL8, April, CX3CL1 and IL-17 will be
 evaluated with ELISA. TH-1, TH-17, TReg and Ly.B cells count will be measured by
 Flowcytometry. MicroRNA-720, MicroRNA-155, MicroRNA-16 and MicroRNA-146a gene expression will
 be measured with Real Time PCR technique.
The purpose of this study was to evaluate the effect of the active ingredient of crucumin on
 the reduction of symptoms of osteoarthritis. Of the 30 patients participating in this study,
 a blood sample will be taken at the beginning of the study, and then after three months of
 taking the crucumin. To evaluate the response to treatment, after each person sampling, we
 will analyze cell count for Th-1, Th-17, TReg and Ly.B by flowcytomtry method. Also we will
 measure cytokines level of IL-17, CXCL8, CX3CL1 and April by ELISA method. Finally we
 evaluate the gene expression of MicroRNA-720, MicroRNA-155, MicroRNA-146a and MicroRNA-16 by
 Real Time PCR technique.
Inclusion Criteria:

 Osteoarthritis patients who have been diagnosed with a rheumatologist physician based on
 clinical examinations and laboratory tests

 Exclusion Criteria:

 Affected by any other acute or chronic underlying disease