Diet Induced Weight Loss to Reduce Inflammation in Obese Women
Diet Induced Weight Loss to Reduce Inflammation in Obese Women
Breast cancer is one of the most frequently seen cancers in the United States. It occurs at
 all ages but is particularly common in post menopausal women. Obesity increases the risk of
 breast cancer and colon cancer among others, and when cancer develops increases the risk of
 spread and death. Inflammation of fat tissue, the coronary blood vessels and the liver are
 also seen with obesity. Animal experiments have shown the inflammation in fat tissue
 increases the production of estrogen. Thus, reducing inflammation in fat tissue might lower
 estrogen levels and reduce the risk of breast cancer in obese women as well as the spread of
 other cancers in the body. Weight reduction in obesity has been shown in epidemiology studies
 to lower the risk of colon cancer and in obese women to lower the risk of breast cancer.
 However, how that occurs and how much weight loss is necessary is not known. In mice, calorie
 restriction in obese animals has been shown to reduce inflammation in fat tissue and the
 breast. In other studies, calorie reduction has been shown to lower the development of
 cancer. In addition, we really do not know what starts the whole inflammation process. One
 good possibility is that immune factors that tend to reduce inflammation are less in obesity.
 We have shown this in the colon and this also has been suggested as occurring in fat stores.
This study aims to determine if weight loss of about 10% of initial weight lowers evidence of
 inflammation in fat stores. It is likely that, if fat store inflammation is reduced, then
 inflammation in breast fat also will be lower. Also, it is possible that blood immune cells
 may be changed with weight loss and even that immune cells in skin will be affected. Since
 vitamin D has important immune effects and vitamin D is low in obesity, we also want to study
 what happens to this vitamin during weight loss.

 This pilot study of weight loss will be done in 10 very obese post menopausal women. This
 study will include nutritional and medical evaluation, a 3 day inpatient hospital stay eating
 a diet providing 50% of what they were taking before starting the study and then a
 nutritionally adequate diet that will allow them to lose about 10% of their initial weight
 within 7 to 10 week period. They will have about 4-5 grams of fat removed by suction through
 a syringe and a biopsy of the skin in addition to studies of blood and stool samples.

 When they have completed the study with a 10% body weight loss they will be referred to a
 nutrition clinic which can counsel them to continue a slower weight loss to an optimal level.
Inclusion Criteria:

 -  40-70 years of age

 -  Post-menopausal women defined as: 24 consecutive months without a menstrual period,
 currently not taking any medication known to induce amenorhea

 -  Body Mass Index 35-50

 Exclusion Criteria:

 -  History of any bleeding disorder

 -  HIV positive

 -  History of previous weight loss surgery.

 -  History of Inflammatory Bowel Disease

 -  History of any other malignancy other than non-melanoma skin cancer in the past 5
 years

 -  Currently taking fish oil, omega-3 supplements or other herbal supplements that exceed
 GRAS (Generally Recognized as Safe) levels

 -  Currently taking any estrogen/progesterone hormones except vaginal cream

 -  Smokers (or stopped < 3 months ago)

 -  Currently taking any medication that can alter fat stores as determined by the
 principal investigator

 -  Currently taking any weight control medication

 -  Currently taking hypoglycemic medications.

 -  Currently taking NSAIDS, aspirin, (if > once a week, stopped <30 days ago). Aspirin
 81mg may be permitted if the Framingham Risk Score is < 10

 -  Currently taking anticoagulant medication or stopped <30 days ago.

 -  Screening fasting blood glucose >165mg/dL

 -  Screening thyroid function test abnormal

 -  Screening LFT results > 2X upper limit of normal

 -  Screening creatinine > 2X upper limit of normal

 -  Any condition or situation which, in the opinion of the investigator, puts the patient
 at significant risk, could complicate the study results, or may interfere
 significantly with participation in the study.