Fecal Transplantation for Inflammatory Bowel Disease
Fecal Transplantation for Inflammatory Bowel Disease
Fecal microbial treatment (FMT) is a treatment that utilizes the microbiota of a healthy
 intestine as a probiotic preparation. The feces of a healthy individual is fluidized and than
 inserted into the intestinal tract of a sick individual, assuming that the healthy flora will
 colonize and cure the intestine. There are reports of the efficacy of this treatment for
 inflammatory bowel disease but currently the numbers are small.

 aim: To investigate whether use of FMT will bring improvement for colitis in IBD patients .
 Methods: The patients will undergo full colonoscopy, the stool preparation will be infused
 through the colonoscope during withdrawal of the instrument.
Back ground: intestinal microbiota have a central role in disease pathogenesis, either in a
 form of a "permissive" role or as a direct pathogenic cause.

 Clostridium difficile colitis; irritable bowel syndrome (IBS) and IBD have all been connected
 to a disturbance in the equilibrium of intestinal microbiome. But the causative mechanism is
 not yet known. (5-10) . Various bacteria including lactobacillus acidophilus and
 bifidobacterium bifidus are being empirically used as treatment for many intestinal ailments.
 The cause of inflammatory bowel disease in unknown but evidence is gathering that excessive
 immune reaction of intestinal immune system to microflora combined with a genetic
 predisposition are responsible for the chronic inflammation.

 Fecal microbial treatment (FMT) is a treatment that utilizes the microbiota of a healthy
 intestine as a probiotic preparation. The feces of a healthy individual is fluidized and that
 inserted into the intestinal tract of a sick individual, assuming that the healthy flora will
 colonize and cure the intestine. Previous work had shown success in fecal transplantation as
 a treatment for clostridium difficile colitis. There are also reports of the efficacy of this
 treatment for inflammatory bowel disease but currently the numbers are small. A review by
 Anderson et al describes 17 studies reporting treatment of IBD by FMT. (12). In 9 cases it
 was done to treat IBD and in 8 cases for intestinal infection in IBD patients. Altogether 41
 cases were reported , In some the FMT was inserted through a nasogastric tube directly to the
 duodenum, in some be colonoscopy and in some by an enema. A significant clinical improvement
 was reported in 19/25 patients. 13/17 stopped IBD treatment , 15/24 entered full clinical
 remission. In all 15 patients treated for infection the treatment was successful. No sever
 adverse effects were reported, Fever was developed in 8 cases and in one case there was
 exacerbation of colitis after treatment.

 Primary aim: To investigate whether use of FMT will bring improvement of at least 2 points in
 partial mayo score in ulcerative colitis patients, or 70 points in CDAI of patients with
 Crohn's colitis. One month after FMT.

 Improvement will be defined as:

 For Ulcerative colitis: a decrease of at least 2 points in the partial mayo score, and a
 decrease of at least 1 point in endoscopic Mayo score.

 For Crohn's disease: A decrease of at least 70 points in Crohn's disease activity index
 (CDAI) .

 Materials and methods:

 Study population: 40 patients aged 18-80 years, with histological and endoscopic diagnosis of
 ulcerative colitis or crohn's colitis who did not respond to either thiopurines or tumor
 necrosis factor (TNF) inhibitors.

 Exacerbation will be defined as partial mayo score higher then 3, with either CRP higher than
 0.5 or endoscopic mayo score >1 in ulcerative colitis and CDAI higher them 220 and C reactive
 protein (CRP) higher than 0.5 in Crohn's colitis.

 Stool will be donated by the patients choice either from a relative, preferably a partner to
 minimize possible transference of an infective agent, alternatively samples will be ordered
 from "open biom".

 Endoscopic disease activity will be defined as follows:

 Endoscopic Mayo score:

 -  Normal or inactive disease: 0

 -  Mild disease (erythema, decreased vascular pattern, mild friability): 1

 -  Moderate disease (marked erythema, absent vascular pattern, friability, erosions): 2

 -  Severe disease (spontaneous bleeding, ulceration): 3

 Simple endoscopic score Crohns disease (SES-CD):

 Segments of intestine: Rectum,Sigmoid and left colon, Transverse colon,Right colon, Ileum for
 each segment the Presence of: ulcers,Ulcerated surface, Affected surface, Presence of
 narrowing, and number of affected segments will be evaluated and the total score sumed, The
 SES-CD will than be calculated as follows: TOTAL - 1.4 X (number of affected segments) =
 E-CDI

 Inclusion criteria

 Donor:

 1. Signed informed consent

 2. Basically healthy, no hospitalization in the last 6 months, no active malignant disease
 (with the exclusion of Basal cell skin tumor that was treated).

 3. No antibiotic treatment in the last 6 months

 4. Preferably a partner of the patient, or a relative leaving in the same house

 5. No febrile illness in the last month

 6. Normal laboratory tests including: blood count, liver and kidney function, negative
 hepatitis A immunoglobulin M (IgM), negative hepatitis C antibody, negative HbsAg,
 negative HIV , human T-lymphotropic virus (HTLV I/II), negative venereal disease
 research laboratory ( VDRL).

 7. Stool tests: Negative stool culture, negative C. difficile toxins (A and B). Negative
 parasite microscopy. Fecal calprotectin.

 Patient:

 1. Signed informed consent

 2. Inflammatory bowel disease diagnosed at least 3 months ago

 3. Failure of either one immunomodulator of at least 3 months duration, or TNF inhibitor
 full induction treatment, or intolerance to either of these drugs.

 4. Currently active disease, partial Mayo score ≥4 for ulcerative colitis (UC), or CDAI
 ≥200 for Crohn's disease (CD).

 5. negative HIV , HTLV I/II, negative stool culture, Negative C diff toxin, negative
 cytomegalovirus ( CMV)

 Treatment protocol:

 1. Both donor and recipient will sign informed consent

 2. Both donor and recipient will do the above mentioned laboratory tests, the investigator
 will verify that the results are negative.

 3. Recipient will be prepared for colonoscopy with 3 liters of polyethylene glycol
 according to the routine protocol.

 4. The donor will bring same day stool, alternatively, preparation from "open biom" will be
 used.

 5. 200 gr stools will be mixed with sterile 200ml sodium chloride (NACL) 0.9% and then
 filtered to exclude large particles.

 6. The patients will undergo full colonoscopy, the stool preparation will be infused
 through the colonoscope during withdrawal of the instrument.

 7. If no improvement within 7 days, the patient will be offered the option of another FMT
 using enema prepared in a similar manner.

 8. Patients will be instructed to maintain a high fiber diet,

 Follow up:

 On first colonoscopy:

 Exact description of the length of the involved segment + Mayo visual score for UC and simple
 endoscopic score - CD (SES -CD) for Crohn's disease.

 2 biopsies from each colonic segment: Rt colon, transverse colon, left colon, rectum.

 Weekly partial Mayo score or Harvey Bradshow Index (HBI ) in the first month, then once a
 month for 6 months.

 After 6 months repeat colonoscopy + biopsies + stool samples. Colonoscopy does not have to be
 complete but has to include the segment that was inflamed in the first colonoscopy.

 At the time of colonoscopy blood tests including complete blood count (CBC), liver and kidney
 function, CRP, fecal calprotectin.

 Microbiota profiles:

 1. From both the donor and the recipient before FMT.

 2. From the recipient: one month and 6 months after FMT. Stool samples will be frozen
 immediately at -20° and then transferred to the laboratory for microbiota analysis.
Inclusion Criteria:

 1. Signed informed consent

 2. Inflammatory bowel disease diagnosed at least 3 months ago

 3. Failure of either one immunomodulator of at least 3 months duration, or TNF inhibitor
 full induction treatment, or intolerance to either of these drugs.

 4. Currently active disease, partial Mayo score ≥4 for ulcerative colitis, or CDAI ≥200
 for CD.

 5. negative HIV , HTLV I/II, negative stool culture, Negative C diff toxin, negative CMV

 Exclusion Criteria:

 1. No informed consent

 2. Non active inflammatory bowel disease.

 3. Active infection in either the donor or the recipient,