Efficacy of Amiloride and Hypertonic Saline in Cystic Fibrosis
Efficacy of Amiloride and Hypertonic Saline in Cystic Fibrosis
The purpose of this research study is to determine whether the combination of inhaled
 amiloride and a concentrated salt solution is better than the salt solution itself for cystic
 fibrosis (CF) patients. In CF, airway secretions are thick and dehydrated. Many patients use
 inhaled salt solutions to help draw water into their secretions so that they are easier to
 get rid of with chest physiotherapy ("chest PT") and cough. Unfortunately, these salt
 solutions are reabsorbed very quickly by the airways, so the beneficial effects may not last
 very long. In the hopes of prolonging their effects, the drug amiloride could be used in
 combination to slow salt and water reabsorption from airways. Amiloride is a medication that
 has been given by mouth for high blood pressure for many years. It is possible that the
 combination of salt solutions and inhaled amiloride may significantly improve the clearance
 of secretions in CF, which would be expected to improve lung function in CF.
Inclusion Criteria:

 -  Established diagnosis of CF

 -  2 gene mutations identified, or

 -  Sweat chloride > 60 mmol/L, and

 -  1 or more typical CF clinical features

 -  Age > 14 years

 -  Able to perform spirometry and have post-bronchodilator FEV1 > 50% of predicted at
 screening

 -  Oxyhemoglobin saturation (by pulse oximetry) > 92% on room air

 -  Able to provide informed consent

 Exclusion Criteria:

 -  Unstable lung disease:

 -  FEV1 > 15% below best clinical measurement within 6 months

 -  Requirement for IV antibiotics within 4 weeks of screening

 -  Requirement for any change in pulmonary medication within 2 weeks of screening

 -  Evidence of reactive airways

 -  Clinical diagnosis of asthma

 -> 15% increase in FEV1 after bronchodilator at screening

 -  Hypertonic saline use within 2 weeks of screening

 -  Unwilling or unable to either continue or discontinue cyclical therapies (e.g. inhaled
 tobramycin) for the 2 weeks prior to screening and the entire study period

 -  Pregnancy, breast-feeding, or unwillingness to use barrier contraception during the
 entire study period

 -  History of allergy or intolerance to amiloride, hypertonic saline, quinine, albuterol,
 or related compounds

 -  Renal insufficiency (creatinine > 1.5 mg/dl)

 -  Hyperkalemia (K+ > 5.0 meq/L)

 -  Investigational drug use within 30 days of screening

 -  Radiation exposure within the past year that would exceed Federal Regulations by
 participating in the study