Diagnostic and Prognostic Value of p16INK4a Expression in Low Grade Squamous Intraepithelial Lesions of the Cervix.
Diagnostic and Prognostic Value of p16INK4a Expression in Low Grade Squamous Intraepithelial Lesions of the Cervix.
P16INK4a has recently been described as a surrogate marker for HR-HPV associated squamous and
 glandular intraepithelial lesions of the cervix. The immunohistochemical staining pattern of
 p16INK4a in high grade intraepithelial neoplasia of the cervix (CIN 2 and 3) is diffuse,
 whereas in CIN 1 different staining patterns (diffuse, sporadic, focal or negative) can be
 seen. The aim of our study is to find out whether the p16INK4a staining pattern of CIN 1 is
 able to predict the outcome of the lesion. The retrospective part of the study includes
 cervical biopsies of 200 patients with CIN 1 and clinical follow-up for at least 5 years.
 p16INK4a staining and HPV detection by IHC will be correlated to clinical outcome.The
 prospective part of the study includes 300 patients with CIN 1 and LSIL on cytology. HPV
 detection by HCII and p16INK4a immunohistochemistry on liquid based cytology samples as well
 as p16INK4a staining and HPV detection by ISH on colposcopy guided biopsies will be
 correlated to clinical follow-up and colposcopy findings. Slides are analysed by 2
 pathologists without knowledge of clinical data.
Responsible women > 18 years with histologically proven CIN 1