Standard 24-hour Urine Protein vs Shorter Period for Diagnosis of Pre-eclampsia
Standard 24-hour Urine Protein vs Shorter Period for Diagnosis of Pre-eclampsia
Background and project rationale:

 Preeclampsia is a common complication of pregnancy, affecting 6-8% of all pregnancies and
 constitutes a leading cause of maternal morbidity and mortality. Preeclampsia is liable to
 endanger the lives of both the gravida and the fetus, particularly if treatment is initiated
 inappropriately or in an untimely fashion.

 Diagnosis of preeclampsia is dependent on the finding of proteinuria, determined as being
 over 300mg of protein in a 24 hours urine sample. However, urine collection spanning 24 hours
 sometimes constitutes a "bottleneck", extending the time to diagnosis of preeclampsia.
 Additionally, the collection of urine for 24 hours entails a degree of discomfort, requiring
 that the woman be in proximity to for collection vessel, and increases the length of her
 hospital admission.

 The use of an abbreviated test may permit diagnosis and treatment in a more timely fashion.
 Similarly, the ability to exclude the diagnosis more rapidly could reduce length of hospital
 stay and consumption of the health system's limited resources. Further, a shorter test may
 reduce the discomfort associated with the 24-hour test and thus increase compliance.

 Previous research has suggested that briefer tests correlate with the traditional 24 hour
 urine collection, however these studies were based on small study populations.

 Research Objective:

 To validate a brief and rapid test for the diagnosis of urinary protein excretion.

 To assess whether, in women with suspected preeclampsia, a difference exists between protein
 excretion during the daytime and at night.

 Methods:

 Urine collection will be performed on pregnant women admitted for investigation of suspected
 preeclampsia, with volumes recorded and samples taken at 6, 12 and 24 hour intervals for
 assessment of urinary protein content. As such, a comparison will be made between the protein
 excretion after 6 and 12 hours with that over a full 24 hour period; in addition, comparison
 will be made between daytime and nighttime urinary protein excretion. The results will allow
 for assessment of whether a shorter test can substitute the full 24 hour collection in the
 diagnosis of preeclampsia; results of women who are shown to not suffer from preeclampsia
 will be used to assess whether a short test can rule out the disease. Additionally a urine
 sample for protein/creatinine ratio will be examined and correlated with results of the
 different collection periods.
Literature review and project rationale:

 Preeclampsia is a common complication of pregnancy, affecting 6-8% of all pregnancies and
 constitutes a leading cause of maternal morbidity and mortality. Preeclampsia is liable to
 endanger the lives of both the gravida and the fetus, particularly if treatment is initiated
 inappropriately or in an untimely fashion.

 The diagnosis of preeclampsia is based on findings of raised blood pressure (over 140/90mmHg)
 and the presence of proteinuria. The accepted means of measuring proteinuria is by 24-hour
 urine collection, with a pathological value defined as 300mg or greater in the 24 hour
 period. When the proteinuria is greater than 5000mg in 24 hours, the pr-eclampsia is
 classified as severe and is further endangers the lives of the expectant mother and fetus and
 often results in early delivery.

 Diagnosis and assessment of severity of preeclampsia require a timely situation assessment.
 Urine collection spanning 24 hours sometimes constitutes a "bottleneck", extending the time
 to diagnosis of preeclampsia. Additionally, the collection of urine for 24 hours entails a
 degree of discomfort, requiring that the woman be in proximity to for collection vessel, and
 increases the length of her hospital admission.

 The use of an abbreviated test may permit diagnosis and treatment in a more timely fashion.
 Similarly, the ability to exclude the diagnosis more rapidly could reduce length of hospital
 stay and consumption of the health system's limited resources. Further, a shorter test may
 reduce the discomfort associated with the 24-hour test and thus increase compliance.

 Previous research has shown that 8-hour urine collection does indeed produce results
 paralleling the 24-hour collection, and recent research also demonstrates concordance between
 12-hour and 24-hour urine collections. That said, both these studies were based on small
 sample populations, fewer than 100 women. Another study of women with preeclampsia showed
 good correlation between the results of urinary protein in samples collected over 12 hours at
 night and those collected over 12 hours during daytime.

 Research Assumptions:

 1. urine collection for a period of time less than 24 hours will reflect the degree of
 urinary protein excretion.

 2. urine collection for a period of time less than 24 hours will constitute an alternative
 test for 24-hour urine collection, the accepted diagnostic test today.

 Research Objective:

 To validate a brief and rapid test for the diagnosis of urinary protein excretion.

 To assess whether, in women with suspected preeclampsia, a difference exists between protein
 excretion during the daytime and at night.

 Methods:

 Urine collection is to be performed on every pregnant admitted for investigation of suspected
 preeclampsia. After 6, 12 and 24 hours samples of the collected urine will be taken for
 protein concentration assessment. Quantitative urinary protein will be established according
 to urinary protein concentration and collected urine volume. Results of the tests after 6 and
 12 hours will be compared to the final results after 24 hours, that being the test of choice
 for diagnosis of proteinuria in preeclampsia and thus the test that will determine management
 (according to department protocol).

 Women for whom preeclampsia has been ruled out (viz, those who underwent investigation
 following raised BP but without a finding of proteinuria) will serve to assess the ability of
 a short urine collection to rule out proteinuria and, as such, preeclampsia.

 So as to assess whether there is a difference between results of daytime urinary protein
 excretion and those at night, the urine collection will be performed in two vessels as
 follows:

 1. 8:00am - beginning of urine collection

 2. 2:00pm, vessel 1 - recording of collected urine volume, removal of a 10mL sample of
 urine for protein concentration testing.

 3. 8:00pm, vessel 1 - recording of total collected urine volume, removal of a 10mL sample
 of urine for protein concentration testing.

 at this point urine collection will be continued in the second vessel

 4. 8:00am the following morning, vessel 2 - recording of collected urine volume as of
 8:00pm (the previous 12 hours), removal of a 10mL sample of urine for protein
 concentration testing.

 5. contents of the two vessels are mixed, 24 hour urine volume recorded and a 10mL sample
 taken, this being the sample that will establish the result of the 24 hour urine
 collection.

 Additionally a urine sample for protein/creatinine ratio will be examined and correlated with
 results of the different collection periods.
Inclusion Criteria:

 -  pregnant women

 -  age 18-55 years

 -  blood pressure >140mmHg systolic or >90mmHg diastolic

 Exclusion Criteria:

 -  known renal disease

 -  urinary tract infection

 -  spontaneous labor or need to induce labor within the first 24 hours of admission