Mobilization and Handling of Stem Cells for Transplant From Healthy Volunteers With Sickle Cell Trait
Mobilization and Handling of Stem Cells for Transplant From Healthy Volunteers With Sickle Cell Trait
This study will examine the effects of granulocyte colony-stimulating factor (G-CSF) on bone
 marrow stem cells in healthy volunteers with sickle cell trait and determine if cells
 collected for transplantation from donors with sickle cell trait require special handling.

 Stem cells, which the bone marrow produces, are responsible for making all the different
 kinds of blood cells. They are the cells used in bone marrow, or stem cell, transplantation.
 The drug G-CSF, which is a naturally occurring hormone, causes stem cells to mobilize-that
 is, to be released from the bone marrow and enter the blood stream. This drug is given to
 stem cell donors to increase the amount of cells that can be collected. Stem cell donors for
 patients with sickle cell disease are often healthy siblings of the patient who have a
 matching bone marrow type. Some siblings carry the sickle cell trait, however, and, even
 though they do not have sickle cell disease and their blood and bone marrow are normal, it is
 not known how their cells will react to G-CSF stimulation. Nor is it known if their stem
 cells require special methods of removal, processing or storing.

 Healthy volunteers 18 years or older with sickle cell trait who have no history of sickle
 cell disease and no known medical problems may be eligible for this study. Participants will
 have a medical history and physical examination, including blood tests and urinalysis. They
 will receive injections of G-CSF under the skin once a day for 5 days. On the fifth day, stem
 cells will be collected through leukapheresis. In this procedure, whole blood is drawn from
 an arm vein, similar to donating whole blood. The blood then circulates through a cell
 separator machine, the stem cells are removed, and the rest of the blood is transfused back
 to the donor through a vein in the other arm.

 The information gained from this study will be used to ensure the safety of stem cell donors
 with sickle cell trait and to better prepare stem cells for transplantation in sickle cell
 patients.
Patients with sickle cell disease are increasingly becoming eligible for transplantation as
 the techniques of stem cell transplantation continue to improve, leading to less morbidity
 and mortality. However, the availability of a matched donor still remains one of the main
 obstacles. Given the inherited nature of this disease, many HLA-matched siblings of patients
 with sickle cell disease have sickle cell trait. Therefore, patients with sickle cell trait
 presumably have been used as donors for patients with sickle cell disease. However, there are
 no published data on whether special manipulations are required for the maintenance and
 storage of stem cells collected from patients with sickle cell trait. The effects of
 granulocyte-colony stimulating factor (G-CSF)-induced stem cell mobilization in these
 patients are also not known. Also, due to the potential of HbS gellation in red cells, the
 freezing of stem cells derived from individuals with sickle cell trait may require a more
 stringent red cell removal than is normally performed. We would like to evaluate these issues
 using volunteers with sickle cell trait prior to beginning our own transplant protocol for
 sickle cell disease.
Confirmed diagnosis of sickle cell trait.

 Greater than age 17.

 No active systemic infection or history of recurrent infections requiring intravenous
 antibiotic treatment .

 Normal renal function: creatinine less than 2X normal, or proteinuria less than 1+.

 Normal liver function: bilirubin less than 2X normal, transaminases within normal limits.

 Normal blood count: WBC 3,000-10,000/mm3, granulocytes greater than 1,500/mm3, platelets
 greater than 150,000/mm3, hemoglobin greater than 12.5g/dL, MCV and MCHC within normal
 limits.

 Female volunteers of childbearing age should have a negative serum pregnancy test within
 one week of beginning G-CSF administration.

 Eligible for normal blood donation (i.e. tested negative for syphilis (RPR), hepatitis B
 and C (Hasbro, Anti-Hubcap, Anti-HCV), HIV, and HTLV-1.

 Subject must give informed consent to participate in the protocol.

 No history of sickle cell crises.

 Children less than 18 years of age are excluded as is per routine for normal volunteer
 protocols.

 No active systemic viral, bacterial, fungal, or parasitic infection.

 No female volunteers with positive pregnancy test or who are lactating.

 No history of autoimmune disease, such as rheumatoid arthritis and systemic lupus
 erythematosus.

 No history of cancer excluding squamous or basal cell carcinoma of the skin.

 No history of any malignant hematologic disorders.

 No history of cardiovascular disease or related symptoms such as chest pain and shortness
 of breath..

 No allergy to G-CSF or bacterial E. coli products.

 No history of G-CSF administration or leukapheresis.