Diagnostic Study of Patients With Aggressive Non-Hodgkin's Lymphoma
Diagnostic Study of Patients With Aggressive Non-Hodgkin's Lymphoma
RATIONALE: Determination of genetic markers for aggressive non-Hodgkin's lymphoma may help
 identify patients with this disease and help predict the outcome of treatment.

 PURPOSE: Diagnostic study to determine the genetic markers in patients who have aggressive
 non-Hodgkin's lymphoma.
OBJECTIVES:

 -  Estimate the proportions of patients with diffuse aggressive non-Hodgkin's lymphoma who
 have rearrangements affecting the MYC, BCL2, and BCL6 genes (determined by fluorescent
 in situ hybridization), overtly amplified chromosomal regions, and nonrandom copy number
 changes of chromosomal regions (determined by comparative genomic hybridization).

 -  Investigate the prognostic importance of these genetic markers by studying their
 relationships with clinical outcomes (e.g., response to therapy, failure-free survival,
 and overall survival) in these patients.

 -  Investigate the interrelationships among these genetic and biological markers and their
 relationships with clinical features of the disease, such as disease site (nodal vs
 extranodal) and stage, in these patients.

 OUTLINE: This is a multicenter study.

 Tissue samples are obtained before treatment and at the time of documentation of refractory
 disease in patients who do not achieve complete remission after induction therapy or at the
 time of first relapse in patients who achieve a complete remission.

 Samples are examined for rearrangements in the MYC, BCL2, BCL6, and IGH genes using
 fluorescent in situ hybridization. DNA is examined by comparative genomic hybridization,
 which allows cytogenetic detection of losses and gains of chromosomal regions in tumor cells.

 Patients do not receive the results of the genetic testing and the results do not influence
 the type or duration of treatment.
DISEASE CHARACTERISTICS:

 -  Histologically proven aggressive non-Hodgkin's lymphoma and registered to front-line
 CALGB protocols (e.g, CLB-59903, 59909, 10002, and 50103)

 -  Diffuse small cleaved cell lymphoma

 -  Diffuse mixed small and large cell lymphoma

 -  Diffuse large cell lymphoma

 -  Diffuse large cell immunoblastic lymphoma

 -  Small noncleaved cell lymphoma

 -  Mantle cell lymphoma OR

 -  Previously entered on similar curative CALGB protocols (CLB-8852, CLB-8854, and
 CLB-9351) with blocks or unstained slides of initial diagnosis available

 PATIENT CHARACTERISTICS:

 Age:

 -  18 years and older