Efficacy of Fluticasone Propionate Associated With Salmeterol Using Inhalation Chamber Versus Placebo to Improve the Respiratory Function in Children Over Six Years of Age Who Underwent Allogeneic Hematopoietic Stem Cell Transplantation With a Decline of FEV1 ≥10% From Pre Transplantation
Efficacy of Fluticasone Propionate Associated With Salmeterol Using Inhalation Chamber Versus Placebo to Improve the Respiratory Function in Children Over Six Years of Age Who Underwent Allogeneic Hematopoietic Stem Cell Transplantation With a Decline of FEV1 ≥10% From Pre Transplantation
Bronchiolitis Obliterative Syndrome (BOS) is the primary noninfectious pulmonary complication
 after hematopoietic stem cell transplantation (HSCT) and usually carries a poor prognosis. It
 occurs in about 10% of children underwent HSCT. The National Institutes of Health (NIH)
 published guidelines and criteria for the diagnosis of BOS. BOS defined by spirometric
 criteria according to modified NIH consensus guidelines: FEV1 < 75% predicted and a greater
 than 10% decline from pretransplant baseline, and FEV1/FVC <0.7 (FCV: Forced Vital Capacity).
 Nevertheless Cheng and al. indicate that the magnitude of FEV1 decline before diagnosis
 exceeded the diagnostic requirement of a greater than 10% decline compared with baseline FEV.
 Moreover, the decline in FEV1 prior to BOS diagnosis appeared to occur within 6 months for
 those patients. Recent studies suggest that any intervention should be targeted during the
 FEV1 decline, and before the diagnosis of BOS. For this, inhalated treatment are used:
 Bergeron et al. reported improvements in symptoms as well in FEV1 one month followed
 treatment including formoterol and budesonide in a prospective trial including adults (12%
 increase of FEV1 for 62% adults). Williams and al. in another prospective adult's cohort,
 showed that the association between fluticasone, montelukast and azythromycin was associated
 with stable lung function, reduced systemic corticosteroids, and improved quality of life at
 3 months for adults with BOS.

 In our national French prospective cohort which include 300 children with HSCT from 2014 to
 2017 (RESPPEDHEM Programme Hospitalier de Recherche Clinique 2012), 35% of children presented
 a decline of FEV1≥ 10% without BOS criteria (FEV1 < 75% and FEV1/FVC <0.7). Among them, some
 received combination of corticoids and long acting beta agonists for 6 months. Children with
 this type of inhalated treatment improved their FEV1 to 88.1% predicted while children
 without any treatment have a FEV1 at 80.7% predicted. Our hypothesis is that association of
 Fluticasone Propionate and Salmeterol can be used as a treatment of the decline of FEV1 for
 children and so prevent BOS.
It's a prospective randomized double blind, multicentre, parallel-group in a 1:1 ratio,
 controlled and superiority trial.

 The primary objective is to assess the effect of a Fluticasone Propionate associated with
 Salmeterol using inhalation chamber compared to placebo on respiratory function at 6 months
 in children over six years of age who underwent allogeneic hematopoietic stem cell
 transplantation with a decline of FEV1 ≥ 10% from pre transplantation.

 The primary criterion will be the difference in the FEV1% predicted value from inclusion to 6
 months following the initiation of treatment.

 To assess the effect of a Fluticasone Propionate associated with Salmeterol using inhalation
 chamber compared to placebo in children over six years old underwent allogeneic hematopoietic
 stem cell transplantation with a decline of FEV1 ≥ 10% from pre transplantation baseline to 6
 months following the initiation of treatment, and 6 months after the end of treatment, on
 pulmonary function, respiratory symptoms, Bronchiolitis Obliterative Syndrome (BOS) rate,
 oral steroid exposure and occurrence of infections;

 To assess potential confounding factors associated to the 6-months inhaled treatment efficacy
 from baseline to 12 months, especially:

 - GVHD (Graft Versus Host Disease) occurrence, severity and treatment (steroid dose exposure,
 calcineurins inhibitors dose, second line of GVHD treatment); To assess the safety and
 tolerability of the treatment at baseline, M1, M3 and M6 following the initiation of
 treatment

 All endpoints were collected at baseline, randomization, 1, 3, 6 months during the treatment
 period, and then 3, 6 months after the end of the treatment.

 1. Endpoints relative to respiratory symptoms :

 -  dyspnea will be evaluate at the NYHA (New York Heart Association) scoring;

 -  step test (patient climb up and down step during three minutes at the frequency of
 30/mn) : Respiratory rate, dyspnea Heart rate, SaO2 (oxygen saturation) during the
 exercise.

 2. Endpoints relative to pulmonary function :

 -  FEV1 (Forced expiratory volume) VC (Vital Capacity) , TLC (Total Lung capacity) ,
 RV (Residual volume), FRC (Functional Residual Capacity), sRaw (specific airway
 resistance) DLCO (diffusing capacity of the lungs) using a similar method of
 measurement for static lung volumes (plethysmography)

 -  Number of patients presented with BOS defined as the absence of infection, and FEV1
 < 75% predicted, and the FEV1/ FVC < 0.7, and the RV >120% or air trapping or
 bronchiectasis or small airways thickening on computed tomography

 3. Endpoints relative to GVHD :

 -  Acute and Chronic GVHD manifestations defined on NIH consensus with scoring;

 -  First line of treatment: cumulative dose of corticosteroid systemic exposure and
 cumulative dose of calcineurins inhibitors.

 -  Second line of GVHD treatment exposure will be assessed using the other type of
 treatment.

 4. Endpoints relative to respiratory infections :

 - Number of adverse events.

 5. Endpoints relative to safety and tolerance:

 -  Adverse events during the study period such as treatment tolerance, thrush, death.

 The investigators will study patients over six years of age who underwent allogeneic
 hematopoietic stem cell transplantation with a decline of FEV1 ≥10% from pre transplantation.

 The choice of this range of age is motivated by the fact that PFTs (Pulmonary Function Test)
 requires a degree of cooperation that is age dependent, only children who are 6 years of age
 or older are able to realize reproducible PFTs.

 For children between 6 to 11 years (< 12 years): 50 μg inhaled fluticasone propionate and 25
 μg salmeterol (SERETIDE® 50/25) :two puffs twice a day from randomisation during 6 months
 using inhalation chamber

 - For children between 12 to 17 years (> or = 12 years) : 125 μg inhaled fluticasone
 propionate and 25 μg salmeterol (SERETIDE® 125/25): two puffs twice a day from randomisation
 during 6 months using inhalation chamber For children between 6 to 11 years (< 12 years):
 placebo of SERETIDE® 50/25 :two puffs twice a day from randomisation during 6 months using
 inhalation chamber For children between 12 to 17 years (> or = 12 years) : placebo of
 SERETIDE® 125/25: two puffs twice a day from randomisation during 6 months using inhalation
 chamber
Inclusion Criteria:

 -  Children and adolescent aged 6 to 17 years

 -  Getting an Allo Hematopoietic cell stem transplantation

 -  Provide written informed consent from legal guardian

 -  Covered by medical insurance (social security ou CMU).

 Randomisation criteria:

 - Decline of FEV1 ≥ 10% from pre transplantation between M3 and M12 after the
 transplantation, confirmed over two functional test performed one week apart, without
 Bronchiolitis Obliterative Syndrome international criteria, neither initiation of inhaled
 treatment from transplantation to randomization visit.

 Exclusion Criteria:

 -  Patients with no affiliation to a social security scheme (beneficiary or legal)

 -  Pregnancy

 -  Asthma defined by reversibility with salbutamol (FEV1 > 12% or FEV1> 200ml) under
 inhaled corticosteroids or long acting beta agonists during the last three months

 -  Patients with hypersensitivity to the active substances: salmeterol, fluticasone
 propionate, or to the excipients: norflurane.

 Non-Randomisation criteria :

 -  Viral respiratory infection (fever ≥ 38°C, tachypnea according to age, positive viral
 PCR (Polymerase Chain Reaction) pharyngeal aspiration) during the last month;

 -  Lower respiratory tract infection (fever ≥ 38°C, tachypnea, radiologically or
 echography confirmed pneumonia, sputum) during the last month;

 -  Invasive fungal disease (as defined by European Organisation for Research and
 Treatment of Cancer/Mycoses Study Group consensus group) during the last month.

 -  Potent cytochrome P450 3A4 inhibitors, such as ritonavir, ketoconazole, itraconazole,
 troleandomycin, clarithromycin, nelfinavir and nefazodone.

 -  Corticosteroids or bronchodilatators inhaled treatment after transplantation

 -  Bronchiolitis Obliterative Syndrome