A Study of the Subcutaneous Injection of a Viscous Antibody Solution With Recombinant Human Hyaluronidase
A Study of the Subcutaneous Injection of a Viscous Antibody Solution With Recombinant Human Hyaluronidase
This Phase I, randomized, double-blinded, within-subject controlled, two-way crossover study
 comparing the time to inject (flow rate), safety, and tolerability of a subcutaneously (SC)
 administered, viscous antibody solution of with and without human recombinant hyaluronidase
 (rHuPH20) in volunteer subjects.

 The study hypothesizes that the time required to complete a 20-mL SC injection of a viscous
 antibody with rHuPH20 will be comparable or shorter than the time required for the injection
 without rHuPH20.
Inclusion Criteria:

 1. Volunteers of either sex, age 18 to 65 years, inclusive.

 2. Intact normal skin on both upper extremities without tattoos or potentially obscuring
 pigmentation or lesions.

 3. Vital signs (BP, HR, temperature, respiratory rate) within normal range.

 4. Metabolic panel (e.g., sodium, potassium, chloride, bicarbonate, BUN, creatinine,
 glucose, calcium, AST, ALT, alkaline phosphatase, total bilirubin, albumin, and total
 protein) within normal range within 7 days of injection.

 5. A negative serum or urine pregnancy test (if female of child-bearing potential) within
 7 days of injection.

 6. Female subjects of child-bearing potential must be practicing effective birth control
 or abstinence currently and plan to continue to do so for the duration of the study.

 7. Decision-making capacity and willingness and ability to comply with the requirements
 for full completion of the trial.

 8. Willingness and ability to sign an informed consent document.

 Exclusion Criteria:

 1. Upper extremity edema.

 2. Upper extremity pathology that could interfere with any protocol-specified outcome
 assessment (e.g., cellulitis, lymphatic disorder or prior surgery, preexisting pain
 syndrome, previous mastectomy and/or axillary lymph node dissection, etc.).

 3. Contraindication to an antibody, such as known history of anaphylactic or severe
 systemic reactions.

 4. Known predisposition to renal insufficiency or renal failure, including diabetes
 mellitus, volume depletion, sepsis, paraproteinemia, and subjects receiving known
 nephrotoxic drugs.

 5. Known allergy to hyaluronidase or any other ingredient in the formulation of Hylenex.

 6. Known allergy to bee or vespid venom.

 7. Known coagulopathy.

 8. Pregnancy or breast-feeding woman.

 9. Known clinically significant cardiovascular, gastrointestinal, hepatic, neurological,
 psychiatric, endocrine, cancer, HIV infection, diabetes mellitus, intercurrent illness
 such as influenza, or other major systemic disease that would unduly risk the
 subject's safety or interfere with the interpretation of results.

 10. Participation in a study of any investigational drug or device within 30 days of
 enrollment in this study.