Cerebral Blood Flow Parameters and Neurobehavioral Development in Infants at Term Age
Cerebral Blood Flow Parameters and Neurobehavioral Development in Infants at Term Age
Although several studies have contrasted the hemodynamics of cerebral arteries using cranial
 Doppler ultrasound between full-term and preterm infants, the assessments were mostly
 conducted in the first days of life. Furthermore, the clinically significant hemodynamic
 parameter has been established from a small sample of infants that its validity on other
 populations remains unclear. Therefore, the purpose of this study is twofold:

 1. to examine the hemodynamics of cerebral arteries in full-term and very low birth weight
 preterm infants at term age

 2. to assess the validity of the hemodynamic parameters in relation to concurrent
 neurobehavioral function.

 This study will enroll normal full-term infants and very low birth weight preterm infants who
 have no major neonatal disease. Cranial Doppler ultrasound examination will be performed on
 all infants at term age. Hemodynamic parameters will include peak systolic velocity, end
 diastolic velocity, resistance index and pulsatility index for the bilateral anterior
 cerebral arteries and middle cerebral arteries. Neurobehavioral development will be
 administered on the same day using the Neonatal Neurobehavioral Examination- Chinese version.
We estimate the sample size of this study based on the cerebral blood flow velocity data of
 Kempley's study that showed 7.2 ± 3.5 and 10.7 ± 2.4 cm/s for the MCAs in 13 preterm and 8
 full-term infants, respectively. As the power is set at 0.8 and the α level is at 0.05, at
 least 17 subjects were required for each group. This study will include 20 full-term and 20
 VLBW preterm infants who are born at the National Taiwan University Hospital (NTUH) and the
 Mackay Memorial Hospital (MMH), Taipei, Taiwan. Written informed consent will be obtained
 from the parents before participation in this study.

 Cranial Doppler ultrasound and the NNE-C examinations will be performed within 5 days after
 birth on full-term infants and at post-menstrual age (PMA) of 40 weeks (± 1 week) on preterm
 infants in the baby room. If the examination time occurs after hospital discharge, assessment
 will be conducted in the baby room at NTUH or in the ultrasound examination room at MMH. The
 examinations will be performed when the babies show stable vital signs in daily activities.
 The NNE-C examination will be administered one hour prior to feeding to minimize distress.
 The NNE-C examination will be performed immediately after the ultrasound assessment. The two
 examinations will be performed by different examiners who were blind to each other's results.
 During brain Doppler ultrasound examination, each infant will be placed on the back in a baby
 crib or an incubator with his/her head in midline. Infant's behavioral state will be kept in
 the behavioral state of 1 (quiet sleep), 2 (active sleep), or 3 (drowsy) on Brazelton's
 classification to eliminate the impact on cerebral blood flow velocity.

 Hemodynamic parameters will include PSV, EDV, RI, and PI from bilateral ACAs and MCAs as
 obtained by the pulse wave color Doppler. The ACA will be approached through the anterior
 fontanel on the sagittal plane and its blood flow velocity will be determined at the proximal
 part. The MCA will be approached under the transverse plane by placing the transducer on the
 temporal bone below the zygomatic arch. The flow velocity measurements will be obtained by
 Fourier transform analysis from homogeneous blood flow wave patterns over at least 3 cardiac
 cycles with good clear signals. Those signals that show complete and similar waveforms in the
 adjacent cycles and have high magnitudes with visible margin recognized from the background
 noise will be selected. The RI and PI are the ratio between the different velocities with the
 formulas: RI = (PSV-EDV) / PSV, PI = (PSV-EDV) / mean velocity. The whole ultrasound
 examination will take about 10 minutes.
Fullterm group

 Inclusion Criteria:

 -  birth weight higher than 2,500 g

 -  appropriate for gestational age

 -  gestation age within 38 and 42 weeks

 Exclusion Criteria:

 -  chromosomal or congenital abnormalities

 Preterm group

 Inclusion Criteria:

 -  birth weight less than 1,500 g

 -  gestational age less than 37 weeks

 -  admission at the hospitals within 7 days after birth

 Exclusion Criteria:

 -  chromosomal or congenital abnormalities