FDG-PET-Stratified R-DICEP and R-Beam/ASCT For Diffuse Large B-Cell Lymphoma
FDG-PET-Stratified R-DICEP and R-Beam/ASCT For Diffuse Large B-Cell Lymphoma
The purpose of this study is to evaluate:

 1. whether an imaging test called a PET (Positron emission tomography) scan performed after
 two cycles of standard chemotherapy is able to identify patients who have a high cure
 rate after completing standard chemotherapy alone; and

 2. whether high dose chemotherapy (HDCT) and autologous stem cell transplantation (ASCT)
 when used in combination with an antibody called Rituximab results in high cure rates
 for those patients predicted to do poorly with standard chemotherapy by the PET scan.
BACKGROUND AND RATIONALE:

 Although the addition of Rituximab anti-CD20 monoclonal antibody therapy to standard CHOP
 chemotherapy (R-CHOP) results in a 15-20% increase in absolute EFS rate over CHOP alone for
 DLBCL patients, it is likely that 40-50% of patients with poor prognosis DLBCL will not be
 cured by R-CHOP. High dose chemotherapy (HDCT) and autologous stem cell transplantation
 (ASCT) has been shown to salvage a substantial proportion of patients with relapsed DLBCL. A
 meta-analysis of randomized controlled trials comparing standard-dose chemotherapy (SDCT)
 alone to SDCT followed by HDCT/ASCT as initial treatment for poor prognosis DLBCL
 demonstrated a significant benefit for HDCT over SDCT in terms of EFS and OS. A single centre
 prospective phase II study was conducted in Calgary to assess feasibility, toxicity, and
 efficacy of two cycles of HDCT following just one cycle of CHOP induction therapy for
 patients under 65 years of age who were diagnosed with aggressive histology lymphoma and 2-3
 Age Adjusted International Prognostic Index (AAIPI) risk factors. The study was conducted
 prior to the widespread use of Rituximab plus CHOP (R-CHOP). The treatment protocol involved
 one cycle of standard dose CHOP followed by one cycle of dose-intensive cyclophosphamide 5.25
 g/m2, etoposide 1.05 g/m2, cisplatin 105 mg/m2 (DICEP), then autologous blood stem cell
 collection, followed by one cycle of high dose BCNU 300 mg/m2, etoposide 800 mg/m2, Ara-C
 1600 mg/m2, melphalan 140 mg/m2 (BEAM) and autologous stem cell transplantation (ASCT) and
 radiotherapy to prior bulk. With a median follow up of 49 months, 4 year event free (EFS) and
 overall survival (OS) rates for the 55 patients accrued to the study were 72% (95% CI=60-84%)
 and 79% (95% CI=69-90%), respectively.

 The two major advances in lymphoma management that have occurred since the design of our
 CHOP-DICEP-BEAM/ASCT phase II study are the addition of Rituximab anti-CD20 monoclonal
 antibody therapy to chemotherapy (as described above), and the use of FDG-PET functional
 imaging/response assessment. Several studies have reported the strong correlation of early
 response assessment using FDG-PET with PFS and OS following standard chemotherapy for
 aggressive NHL. In one of the largest, best designed studies, PET-negativity following 2
 cycles of RCHOP was associated with higher 2-year estimates of EFS (82% vs 43% , p < .001)
 and OS (90% vs 61%, p = .006). The incorporation of Rituximab and PET-guided HDCT/ASCT into
 our intensive chemotherapy protocol may lead to further improvements in EFS while decreasing
 the overall need for HDCT/ASCT by targeting those patients destined to fail R-CHOP by early
 PET-based response assessment.

 STUDY OBJECTIVES:

 1. To determine if negative FDG-PET imaging following 2 cycles of R-CHOP chemotherapy is
 able to identify a group of patients with excellent prognosis (80% 3-year EFS) following
 standard 6 cycles of R-CHOP chemotherapy alone.

 2. To determine if a group of patients with positive FDG-PET imaging following 2 cycles of
 R-CHOP chemotherapy are able to achieve excellent outcome (80% 3-year EFS) following
 intensive chemotherapy with R-DICEP and R-BEAM/ASCT.

 3. To determine if PET-guided R-DICEP and R-BEAM/ASCT is feasible and associated with
 acceptable toxicity rates in a multi-centre study.

 STUDY DESIGN:

 INCLUSION CRITERIA:

 1. Give written informed consent

 2. Age 18-65 years

 3. Histological diagnosis of Diffuse Large B-cell Lymphoma including:

 1. Diffuse Large B-cell

 2. Immunoblastic B-cell

 3. Primary Mediastinal Diffuse Large B-cell

 4. T-cell Rich, Diffuse Large B-cell

 5. Anaplastic Large B-Cell

 6. Diffuse Large B-cell with areas of follicular lymphoma in same biopsy

 4. No central nervous system involvement by lymphoma

 5. No more than 1 prior cycle of R-CHOP chemotherapy

 6. At least 1 adverse International Prognostic Index factor at diagnosis:

 a. Age > 60 years b. ECOG performance status 2-4 c. Elevated serum LDH level d. More
 than 1 extranodal site involved by lymphoma e. Stage 3 or 4

 7. Adequate organ function:

 1. Cardiac: LVEF >45%

 2. Pulmonary: FVC, FEV1 and DLCO >50% predicted

 3. Renal: creatinine <120µmol/L unless caused by ureteric obstruction from lymphoma

 4. Liver: ALT, AlkP, Bilirubin <3 x upper limit of normal unless caused by biliary
 tract obstruction from lymphoma

 EXCLUSION CRITERIA:

 1. Histological diagnosis other than Diffuse Large B-cell Lymphoma 2. Pregnant or
 lactating females 3. Concurrent use of other anti-cancer therapies 4. Other serious
 co-morbid illness that would compromise study participation. 5. Prior malignancy
 unless non-melanoma skin cancer, carcinoma in-situ of the cervix (CIN) or breast,
 or malignancy treated more than 5 years previously with no evidence of recurrent
 disease since initial treatment.

 6. More than 1 prior cycle of Rituximab, cyclophosphamide, or doxorubicin. 7. Prior
 HDCT/ASCT or prior radiotherapy.

 STUDY ENDPOINTS

 Primary:

 • 3 year EFS for patients receiving 6 cycles R-CHOP (PET-negative response) and for
 patients receiving RCHOP x2 followed by R-DICEP then R-BEAM/ASCT (PET-positive
 response)

 Secondary:

 • Complete and Partial Response Rates

 • PET/CT response rates following RCHOP x 2 cycles

 • Overall Survival

 -  Safety (type, frequency, severity, and relationship of adverse events to study
 treatment)

 STUDY DURATION: Three years TOTAL SAMPLE SIZE: 70-80 patients

 DOSING REGIMEN(S):

 1. Induction: Standard R-CHOP chemotherapy q21days x 2 cycles

 R-CHOP x 2 cycles q21days:

 Rituximab 375mg/ m2 IV day 1 Cyclophosphamide 750mg/ m2 IV day 1 Adriamycin 50mg/ m2 IV
 day 1 Vincristine 1.4mg/ m2 (max 2mg) IV day 1 Prednisone 100mg po days 1-5

 2. FDG-PET/CT imaging between days 10-15 following R-CHOP cycle #2.

 Sites of disease will be scored for intensity based on the following scale:

 1. Absent

 2. Minimal (low grade but greater than in regional lymph nodes of similar size or
 surrounding interstitial tissues)

 3. Low (readily visible but less than liver)

 4. Medium (comparable to liver)

 5. High (greater than liver)

 Interim post therapy scans will be considered as negative (showing a favorable response)
 if no more than one site shows an intensity score of greater than 3.

 The treatment protocol will be dictated by the result of the interim FDG-PET/CT scan
 following 2 cycles of R-CHOP chemotherapy as follows:

 1. If FDG-PET scan negative: 4 more cycles R-CHOP (total 6 cycles R-CHOP)

 2. If FDG-PET scan positive: one cycle of R-DICEP then within 5-8 weeks administer
 R-BEAM and autologous blood stem cell transplantation as outlined below:

 3) For PET-positive patients ONLY:

 R-DICEP CHEMOTHERAPY (begin 3-5 weeks following day 1 CHOP #2).

 ½NS+20meq KCL/L IV at 150ml/h start 19:00h day 1 then decrease to 75ml/h at 08:00am day
 2, continue to day 5 Rituximab 375mg/m2 IV day 1 and 8 (day 1 on a Wednesday)
 Cyclophosphamide 1.75 g/ m2 d2,3,4 in 500ml D5W over 2 hrs Etoposide 350 mg/ m2 d2,3,4
 in 1 L over 2 hrs daily Cisplatin 35 mg/m2 d2,3,4 in 500ml N/S +25g mannitol over 2 hrs
 Mesna 1.75g/m2 d2,3,4 by continuous infusion over 24 hrs G-CSF 300 (<70kg), 480
 (70-100kg), or 600mcg/d (>100kg) SC from day 15 until apheresis completed

 AUTOLOGOUS BLOOD STEM CELL COLLECTION:

 Apheresis approximately day 20-22 of R-DICEP, after ANC>5 x 109/L, platelets increasing
 >50 x109/L , and peripheral blood CD34 count >20 x106/L (if measurement available).
 Apheresis to proceed according to standards set at each study centre until at least
 3x106CD34+ cells/kg have been collected (>5x106 CD34+ cells/kg preferred).

 R-BEAM + Autologous Stem Cell Transplantation (5-8weeks post-d1 R-DICEP)

 Day - 6 Rituximab 375mg/m2 IV BCNU 300mg/m2 IV Day -5 to -2 Etoposide 100mg/m2 IV q12h x
 8 doses Ara-C 200mg/m2 IV q12h x 8 doses Day -1 Melphalan 140mg/m2 IV Day 0 Stem Cell
 Infusion Day 14 Rituximab 375mg/m2 IV
Inclusion Criteria:

 -  Age 18-65 years

 -  Diagnosis of Diffuse Large B-Cell Lymphoma

 -  Adverse Prognosis = Stage 3 or 4 and elevated LDH

 -  No more than one prior cycle of R-CHOP chemotherapy

 -  Adequate cardiac function

 -  No central nervous system involvement by lymphoma

 Exclusion Criteria:

 -  Histological diagnosis other than Diffuse Large B-cell Lymphoma

 -  Pregnant or lactating females

 -  Use of other anti-cancer therapies

 -  Other serious illness that would compromise study participation

 -  Prior malignancy

 -  Prior stem cell transplant or radiotherapy