Yeast Cells as Antioxidant-Producing Probiotics
Yeast Cells as Antioxidant-Producing Probiotics
The purpose of this study is to evaluate the efficacy of Baker's yeast extractions ,
 probiotic antioxidant, in scavenging the oxidative stress status mediated damage on sperm
 motility,progressive motility and vitality as well as comparing this potency to that of
 Vitamin E in infertile men.
The practical part of the study took place from August 2015 to May 2016. The Study population
 was made up of infertile males attending Hawaa fertility center, banha city, Qaliubiya,
 Egypt.

 40 study samples were collected from patients who indicated willingness to participate in the
 study and have had 3 to 7 days of sexual abstinence, using the masturbation method and
 ejaculated in wide mouthed plastic container as described by World Health Organization (WHO).

 Samples were allowed to liquefy for 20 minutes and were examined for volume, viscosity,
 spermatozoa count by Neubauer haemocytometer , oxidative stress level measurement by
 Oxisperm, percentage of progressive motility and non-progressive motility and sperm vitality.

 The Semen specimens were divided into ten equal fractions. First fraction was control, 0.1ml
 of liquefied semen mixed with 0.1ml Ham's F10 medium and incubated at 37o C for 30 minutes.,
 2nd, 3rd, 4th fractions (Vit. E 1,2,3), 0.1ml liquefied semen was mixed with 0.1ml Ham's F10
 medium supplemented with (1, 2, 5 mg/ml) Vitamin E respectively and 5th, 6th, 7th parts
 fractions (Yeast extraction ( YE) 1,2,3), 0.1ml liquefied semen was mixed with 0.1ml Ham's
 F10 medium supplemented with (10, 20, 50 mg/ml) Yeast extraction and incubated at 37o C for
 30 minutes.

 Vitamin C was obtained from (Sigma, Germany), 8th, 9th, 10th fractions (Vit. C. 1,2,3), 0.1ml
 liquefied semen was mixed with 0.1ml Ham's F10 medium supplemented with (0.02, 0.04, 0.06
 mg/ml) Vitamin C respectively and incubated at 37o C for 30 minutes.

 After semen specimen treated in all fractions, they were examined and assessed for
 microscopical changes, total motility, progressive motility, vitality, level of oxidative
 stress (OS) were detected in all fractions 30 minutes later after addition of the
 antioxidants.
Inclusion Criteria:

 -  must have been married for 1 year before inclusion into the study and were unable to
 achieve pregnancy

 -  must have not received an antibiotic treatment for the last 4 weeks prior to sampling

 -  showed oxidative stress- mediated asthenozoospermia in their semen

 Exclusion Criteria:

 -  Severe male Factor