Pathophysiology of Paget's Disease of Bone
Pathophysiology of Paget's Disease of Bone
Paget's disease of the bone is a skeletal disorder which results in increased and
 disorganized bone remodeling, leading to dense but fragile and expanding bones. The
 identified genetic causes of Paget's disease of bone only explain why bone is destroyed, but
 not why the bone formed in its place is abnormal.

 Current treatment for people with Paget's disease of the bone is limited to patients with
 bone pain, thought to be related to high rate of bone turnover (breakdown and rebuilding of
 bone) and works by slowing down the rate of bone breakdown. The current treatment does not
 address the excess blood vessels and bone formed.

 This research is being done to understand factors that may promote blood vessel and bone
 formation in Paget's disease of the bone.
The genetic mutations found in Paget's disease currently only account for about 15% of cases
 and are limited to genes that affect osteoclast differentiation and function. These mutations
 alone are insufficient to explain the full phenotype, particularly hypervascularity and
 increased bone formation. Through a series of basic science studies, the investigators have
 recently found that preosteoclasts secrete chemokines to promote migration of various stem
 cells, which then differentiate into osteoblasts and endothelial cells to support
 osteogenesis and angiogenesis, respectively.

 The investigators will perform a cross sectional study of patients with active Paget's
 disease of bone compared to similar people without Paget's disease of bone. The goal is to
 enroll 10 patients with Paget's disease of the bone (cases) and 10 healthy, age- and
 sex-matched people (controls) whom meet similar exclusion criteria. Participants who consent
 to the study will undergo a brief history and physical exam, allow review of medical records
 relevant to their disease, and have one blood (5 tablespoons) sample drawn.

 The investigators hypothesize that specific chemokine concentrations are increased in people
 with Paget's disease of the bone compared to controls. The investigators also hypothesize
 that these levels correlate with severity of disease. Therefore, the investigators primary
 objective is to determine if serum chemokine levels are increased in patients with Paget's
 disease of the bone. The secondary objective is to evaluate if the serum chemokine
 concentrations correlate with various markers of disease activity. Findings could aid in the
 clinical monitoring of patients with Paget's disease of the bone and could provide an
 additional therapeutic target to improve treatment of this painful disease.
Inclusion Criteria for Cases:

 -  Men and women between the ages of 18-99 years who have evidence of active Paget's
 disease of bone as clinically and/or radiographically defined by:

 -  Increased serum alkaline phosphatase or increased serum collagen type 1
 c-telopeptide (CTX) or increase in urinary pyridinoline at diagnosis

 -  AND history of at least one of the following signs/symptoms: Pagetoid lesions(s)
 on x-ray/CT/MRI, increased uptake of radioactive substance by bone scan, bone
 pain, fracture, hearing loss, headache, hypercalcemia, or bony deformity.

 Inclusion criteria for controls:

 -  Men and women between the ages of 18-99 years who match age within 5 years of cases
 and gender who do NOT have evidence of Paget's disease of bone as defined by:

 -  No bone pain or bony deformity

 -  Normal serum alkaline phosphatase

 Exclusion Criteria:

 -  Osteosarcoma or other blastic bony metastases alone

 -  Fibrous dysplasia of bone

 -  Hyperostosis frontalis interna

 -  All men and women < 18 years or > 99 years

 -  Pregnancy (women) determined by self-report

 -  Current use of oral contraceptive tablets or Depo-Provera™ (women)

 -  Current use of hormone replacement therapy

 -  Creatinine clearance < 60 ml/min./1.73 m2 by Cockcroft-Gault based on most recent
 serum creatinine level (if greater than 1 year since last assessment, will be measured
 on collected blood sample to verify eligibility)

 -  Current smoking or tobacco use

 -  Alcohol use greater than 3 units daily

 -  Use of thiazolidinediones within the last year

 -  Use of medications known to impact bone and mineral metabolism, including use of a
 bisphosphonate in the last 11 months; ever use of teriparatide or denosumab; use of
 calcitonin, selective estrogen receptor modulators (SERMs), or estrogen within the
 past 6 months, prednisone > 5 mg for over 10 days in the last three months,
 anti-epileptic medications (e.g. phenytoin, carbamezapine, phenobarbitol, and
 primidone); current or use within the past year of aromatase inhibitors; leuprolide;
 histrelin

 -  History of a thyroid problem that is currently uncontrolled as defined by most recent
 thyroid stimulating hormone levels < 0.1 microIU/mL (if greater than 6 months since
 last assessment, will be measured on collected blood sample to verify eligibility)

 -  Other known metabolic or structural bone disease other than low bone density (e.g.
 hyperparathyroidism, multiple myeloma, sarcoid or other granulomatous disease, celiac
 disease, osteopetrosis, osteomalacia, osteitis fibrosa cystica)

 -  Other significant medical illness (heart disease, pulmonary disease, inflammatory
 bowel disease, malignancy other than ductal carcinoma in situ (DCIS) or non-melanoma
 skin cancer, rheumatologic conditions including rheumatoid arthritis, systemic lupus,
 renal disease requiring dialysis, etc.)

 -  Inability to understand and provide informed consent.