Intervention With Lupin Protein-enriched Foods in Hypercholesterolemic Subjects
Intervention With Lupin Protein-enriched Foods in Hypercholesterolemic Subjects
The objective of the study is to elucidate the effects of lupin protein (Lupinus
 angustifolius Boregine) as part of a mixed diet on cardiovascular risk factors and to clarify
 the role of arginine, one of the most abundant amino acids in lupin protein.
The study is based on a previous finding that a daily dosage of 25 g lupin protein isolate,
 administered as protein drinks, is capable to influence the plasma lipids positively.

 Consequently, the physiological effects of a mixed diet containing 25 g lupin protein isolate
 per day will be investigated compared to 1) a diet containing 25 g of milk protein as well as
 to 2) a diet with 25 g milk protein and additionally the amino acid arginine supplemented
 daily.

 A double-blinded, controlled, randomized cross-over trial will be performed. Altogether 75
 volunteers with hypercholesterolemia will be divided into three groups of 25 subjects each.
 After a run-in period (baseline), the first group will consume foods with lupin protein
 isolate (group A), the second group will receive the same foods with milk protein isolate
 (group B) and the third group will consume the foods with milk protein and 1,6 g arginine per
 day over a period of four weeks. After a wash-out period of six weeks, the diet will be
 crossed within the three groups for a second intervention period of four weeks. After another
 wash-out period, diet will be crossed within the three groups once again.

 Arginine will be provided as capsule (1,6 g per day = four capsules per day) in one of the
 two groups receiving foods with milk protein. In the other groups (receiving foods with lupin
 protein or milk protein only) four placebo capsules will be served, containing mannitol.
Inclusion Criteria:

 -  Moderate Hypercholesterolemia (total cholesterol >= 5.2 mmol/L)

 -  Age: 20-80 years

 Exclusion Criteria:

 -  Intake of lipid-lowering pharmaceuticals

 -  Allergy against legumes

 -  Intolerance or allergy against milk

 -  Pregnancy, lactation

 -  Chronic bowel diseases