Evaluating the Safety and Immunogenicity of a Prime-boost Vaccine Regimen of GEO-D02 DNA and MVA/HIV62B With and Without B63521^11 gp120 and IHV01 gp120 Env Proteins in Healthy, HIV-uninfected Adult Participants
Evaluating the Safety and Immunogenicity of a Prime-boost Vaccine Regimen of GEO-D02 DNA and MVA/HIV62B With and Without B63521^11 gp120 and IHV01 gp120 Env Proteins in Healthy, HIV-uninfected Adult Participants
The purpose of this study is to evaluate the safety and immunogenicity of a prime-boost
 vaccine regimen of GEO-D02 DNA and MVA/HIV62B with and without B63521^11 gp120 and IHV01
 gp120 Env proteins in healthy, HIV-uninfected adult participants.
This study will evaluate the safety and immunogenicity of a prime-boost vaccine regimen of
 GEO-D02 DNA and MVA/HIV62B with and without B63521^11 gp120 and IHV01 gp120 Env proteins in
 healthy, HIV-uninfected adult participants.

 Participants will be randomly assigned to one of five groups. Participants in all five groups
 will receive GEO-D02 DNA by intramuscular (IM) injection at Months 0 and 2. Then, at Months
 4, 6, and 10, participants will receive three additional injections according to their
 assigned group:

 -  Group 1: MVA/HIV62B, placebo for B63521^11 gp120, and placebo for IHV01, all by IM
 injection

 -  Group 2: MVA/HIV62B by IM injection and placebo for B63521^11 gp120 and for IHV01, both
 by subcutaneous (SC) injection

 -  Group 3: MVA/HIV62B, B63521^11 gp120, and IHV01, all by IM injection

 -  Group 4: MVA/HIV62B, placebo for B63521^11 gp120, and IHV01, all by IM injection

 -  Group 5: MVA/HIV62B by IM injection and IHV01 and B63521^11 gp120, both by SC injection

 Participants will attend several study visits through Month 16. Visits may include physical
 examinations, blood and urine collection, electrocardiogram, HIV testing, risk reduction
 counseling, and questionnaires. Study staff will contact participants at Month 24 for
 follow-up health monitoring.
Inclusion Criteria

 General and Demographic Criteria

 -  Age of 18 to 50 years

 -  Access to a participating HVTN Clinical Research Site (CRS) and willingness to be
 followed for the planned duration of the study

 -  Ability and willingness to provide informed consent

 -  Assessment of understanding: volunteer demonstrates understanding of this study;
 completes a questionnaire prior to first vaccination with verbal demonstration of
 understanding of all questionnaire items answered incorrectly

 -  Willing to be contacted after completion of scheduled clinic visits for a total of 2
 years following initial study injection

 -  Agrees not to enroll in another study of an investigational research agent while in
 this study

 -  Good general health as shown by medical history, physical exam, and screening
 laboratory tests

 HIV-Related Criteria:

 -  Willingness to receive HIV test results

 -  Willingness to discuss HIV infection risks and amenable to HIV risk reduction
 counseling

 -  Assessed by the clinic staff as being at "low risk" for HIV infection and committed to
 maintaining behavior consistent with low risk of HIV exposure through the last
 required protocol clinic visit (see the study protocol).

 Laboratory Inclusion Values

 Hemogram/Complete blood count (CBC)

 -  Hemoglobin greater than or equal to 11.0 g/dL for volunteers who were assigned female
 sex at birth, greater than or equal to 13.0 g/dL for volunteers who were assigned male
 sex at birth. For transgender participants who have been on hormone therapy for more
 than 6 consecutive months, determine hemoglobin eligibility based on the gender with
 which they identify (ie, a transgender female who has been on hormone therapy for more
 than 6 consecutive months should be assessed for eligibility using the hemoglobin
 parameters for persons assigned female sex at birth).

 -  White blood cell count equal to 2,500 to 12,000 cells/mm^3 with normal differential,
 or differential approved by Investigator of Record (IoR) as not clinically significant

 -  Total lymphocyte count greater than or equal to 650 cells/mm^3 with normal
 differential, or differential approved by IoR as not clinically significant

 -  Remaining differential either within institutional normal range or with site physician
 approval

 -  Platelets equal to 125,000 to 550,000 cells/mm^3

 Chemistry

 -  Chemistry panel: alanine aminotransferase (ALT) less than 1.25 times the institutional
 upper limit of normal; creatinine less than or equal to 1.1 times the institutional
 upper limits of normal.

 HIV Status

 -  Negative HIV-1 and -2 blood test: US volunteers must have a negative U.S. Food and
 Drug Administration (FDA)-approved enzyme immunoassay (EIA).

 Urine

 -  Normal urine:

 -  Negative or trace urine protein, and

 -  Negative, trace, or 1+ blood urine hemoglobin (if +1 hemoglobin is present on
 dipstick, a microscopic urinalysis with red blood cells levels within
 institutional normal range).

 Reproductive Status

 -  Volunteers who were assigned female sex at birth: negative serum or urine beta human
 chorionic gonadotropin (β-HCG) pregnancy test at screening (ie, prior to
 randomization) and prior to study product administration on the day of study product
 administration. Persons who are NOT of reproductive potential due to having undergone
 hysterectomy or bilateral oophorectomy (verified by medical records), are not required
 to undergo pregnancy testing.

 -  Reproductive status: A volunteer who was assigned female sex at birth:

 -  Must agree to consistently use effective contraception (see the study protocol)
 for sexual activity that could lead to pregnancy from at least 21 days prior to
 enrollment until 3 months after the final study vaccination. Effective
 contraception is defined as using the following methods:

 -  Condoms (male or female) with or without a spermicide,

 -  Diaphragm or cervical cap with spermicide,

 -  Intrauterine device (IUD),

 -  Hormonal contraception,

 -  Tubal ligation, or

 -  Any other contraceptive method approved by the HVTN 132 Protocol Safety
 Review Team (PSRT)

 -  Successful vasectomy in any partner assigned male sex at birth (considered
 successful if a volunteer reports that a male partner has [1] documentation
 of azoospermia by microscopy, or [2] a vasectomy more than 2 years ago with
 no resultant pregnancy despite sexual activity postvasectomy);

 -  Or not be of reproductive potential, such as having reached menopause (no menses
 for 1 year) or having undergone hysterectomy, or bilateral oophorectomy;

 -  Or be sexually abstinent.

 -  Volunteers who were assigned female sex at birth must also agree not to seek pregnancy
 through alternative methods, such as artificial insemination or in vitro fertilization
 until after the last required protocol clinic visit.

 Exclusion Criteria

 -  Blood products received within 120 days before first vaccination.

 -  Investigational research agents received within 30 days before first vaccination.

 -  Body mass index (BMI) greater than or equal to 40.

 -  Volunteer has 2 or more of the following cardiac risk factors:

 -  Participant report of history of elevated blood cholesterol defined as fasting
 low-density lipoprotein (LDL) greater than 160 mg/dL;

 -  First degree relative (eg, mother, father, brother, or sister) who had coronary
 artery disease before the age of 50 years;

 -  Current smoker; or

 -  BMI greater than or equal to 35.

 -  Intent to participate in another study of an investigational research agent or any
 other study that requires non-HVTN HIV antibody testing during the planned duration of
 the HVTN 132 study.

 -  Pregnant or breastfeeding.

 -  Active duty and reserve US military personnel.

 Vaccines and other Injections

 -  Smallpox vaccine received within the last 5 years.

 -  HIV vaccine(s) received in a prior HIV vaccine trial. For volunteers who have received
 control/placebo in an HIV vaccine trial, the HVTN 132 PSRT will determine eligibility
 on a case-by-case basis.

 -  Non-HIV experimental vaccine(s) received within the last 1 year in a prior vaccine
 trial. Exceptions may be made by the HVTN 132 PSRT for vaccines that have subsequently
 undergone licensure by the FDA or by the national regulatory authority where the
 volunteer is enrolling. For volunteers who have received control/placebo in an
 experimental vaccine trial, the HVTN 132 PSRT will determine eligibility on a
 case-by-case basis. For volunteers who have received an experimental vaccine(s)
 greater than 1 year ago, eligibility for enrollment will be determined by the HVTN 132
 PSRT on a case-by-case basis.

 -  Live attenuated vaccines received within 30 days before first study injection or
 scheduled within 14 days after the first study injection (eg, measles, mumps, and
 rubella [MMR]; oral polio vaccine [OPV]; varicella; yellow fever; live attenuated
 influenza vaccine.)

 -  Any vaccines that are not live attenuated vaccines and were received within 14 days
 prior to first vaccination (eg, tetanus, pneumococcal, Hepatitis A or B.)

 -  Allergy treatment with antigen injections within 30 days before first vaccination or
 that are scheduled within 14 days after first vaccination.

 Immune System

 -  Immunosuppressive medications received within 168 days before first vaccination (Not
 exclusionary: [1] corticosteroid nasal spray; [2] inhaled corticosteroids; [3] topical
 corticosteroids for mild, uncomplicated dermatologic condition; or [4] a single course
 of oral/parenteral prednisone or equivalent at doses less than or equal to 60 mg/day
 and length of therapy less than 11 days with completion at least 30 days prior to
 enrollment.)

 -  Serious adverse reactions to vaccines or to vaccine components such as mannitol,
 aluminum hydroxide, and egg products, including history of anaphylaxis and related
 symptoms such as hives, respiratory difficulty, angioedema, and/or abdominal pain.
 (Not excluded from participation: a volunteer who had a non-anaphylactic adverse
 reaction to pertussis vaccine as a child.)

 -  Immunoglobulin received within 60 days before first vaccination.

 -  Autoimmune disease, current or history (Not exclusionary: mild, well-controlled
 psoriasis.)

 -  Immunodeficiency.

 Cardiac

 -  History of myocarditis, pericarditis, cardiomyopathy, congestive heart failure with
 permanent sequelae, clinically significant arrhythmia (including any arrhythmia
 requiring medication, treatment, or clinical follow-up.)

 -  Electrocardiography (ECG) with clinically significant findings, or features that would
 interfere with the assessment of myo/pericarditis, as determined by a contract ECG Lab
 or cardiologist, including any of the following: (1) conduction disturbance (complete
 left or complete right bundle branch block or nonspecific intraventricular conduction
 disturbance with QRS greater than or equal to 120 ms, PR interval greater than or
 equal to 220ms, any second or third degree atrioventricular (AV) block, or QTc
 prolongation (greater than 450ms)); (2) repolarization (ST segment or T wave)
 abnormality that will interfere with the assessment of myo/pericarditis; (3)
 significant atrial or ventricular arrhythmia; (4) frequent atrial or ventricular
 ectopy (eg, frequent premature atrial contractions, 2 premature ventricular
 contractions in a row); (5) ST elevation consistent with ischemia; (6) evidence of
 past or evolving myocardial infarction.

 Clinically significant medical conditions

 -  Clinically significant medical condition, physical examination findings, clinically
 significant abnormal laboratory results, or past medical history with clinically
 significant implications for current health. A clinically significant condition or
 process includes but is not limited to:

 -  A process that would affect the immune response,

 -  A process that would require medication that affects the immune response,

 -  Any contraindication to repeated injections or blood draws,

 -  A condition that requires active medical intervention or monitoring to avert
 grave danger to the volunteer's health or well-being during the study period,

 -  A condition or process for which signs or symptoms could be confused with
 reactions to vaccine, or

 -  Any condition specifically listed among the exclusion criteria below.

 -  Any medical, psychiatric, occupational, or other condition that, in the judgment of
 the investigator, would interfere with, or serve as a contraindication to, protocol
 adherence, assessment of safety or reactogenicity, or a volunteer's ability to give
 informed consent.

 -  Psychiatric condition that precludes compliance with the protocol. Specifically
 excluded are persons with psychoses within the past 3 years, ongoing risk for suicide,
 or history of suicide attempt or gesture within the past 3 years.

 -  Current anti-tuberculosis (TB) prophylaxis or therapy.

 -  Asthma other than mild or moderate, well-controlled asthma. (Symptoms of asthma
 severity as defined in the most recent National Asthma Education and Prevention
 Program (NAEPP) Expert Panel report.) Exclude a volunteer who:

 -  Uses a short-acting rescue inhaler (typically a beta 2 agonist) daily, or

 -  Uses high dose inhaled corticosteroids, or

 -  In the past year has had either of the following:

 -  Greater than 1 exacerbation of symptoms treated with oral/parenteral
 corticosteroids;

 -  Needed emergency care, urgent care, hospitalization, or intubation for
 asthma.

 -  Diabetes mellitus type 1 or type 2 (Not exclusionary: type 2 cases controlled with
 diet alone or a history of isolated gestational diabetes.)

 -  Thyroidectomy, or thyroid disease requiring medication during the last 12 months. (Not
 exclusionary: well-controlled non-autoimmune thyroid disease.)

 -  Hypertension:

 -  If a person has been found to have elevated blood pressure or hypertension during
 screening or previously, exclude for blood pressure that is not well controlled.
 Well-controlled blood pressure is defined in this protocol as consistently less
 than or equal to 140 mm Hg systolic and less than or equal to 90 mm Hg diastolic,
 with or without medication, with only isolated, brief instances of higher
 readings, which must be less than or equal to 150 mm Hg systolic and less than or
 equal to 100 mm Hg diastolic. For these volunteers, blood pressure must be less
 than or equal to 140 mm Hg systolic and less than or equal to 90 mm Hg diastolic
 at enrollment.

 -  If a person has NOT been found to have elevated blood pressure or hypertension
 during screening or previously, exclude for systolic blood pressure greater than
 or equal to 150 mm Hg at enrollment or diastolic blood pressure greater than or
 equal to 100 mm Hg at enrollment.

 -  Bleeding disorder (eg, factor deficiency, coagulopathy, or platelet disorder requiring
 special precautions.)

 -  Malignancy (Not excluded from participation: Volunteer who has had malignancy excised
 surgically and who, in the investigator's estimation, has a reasonable assurance of
 sustained cure, or who is unlikely to experience recurrence of malignancy during the
 period of the study.)

 -  Seizure disorder: History of seizure(s) within past three years. Also exclude if
 volunteer has used medications in order to prevent or treat seizure(s) at any time
 within the past 3 years.

 -  Asplenia: any condition resulting in the absence of a functional spleen.

 -  History of generalized urticaria, angioedema, or anaphylaxis. (Not exclusionary:
 angioedema or anaphylaxis to a known trigger with at least 5 years since last reaction
 to demonstrate satisfactory avoidance of trigger.)