Study of the Role of G72 in Amyotrophic Lateral Sclerosis: Biomarker Discovery and Mechanism Investigation
Study of the Role of G72 in Amyotrophic Lateral Sclerosis: Biomarker Discovery and Mechanism Investigation
G72 gene is located on the common linkage locus in bipolar disorder and schizophrenia, and it
 encodes D-amino acid oxidase activator (DAOA). There are evidences that elucidated G72 and
 D-amino acid oxidase(DAO) together playing a critical role in the pathophysiology of
 schizophrenia. Recently, reports discovered missense mutations in the DAO (R199W DAO and R38H
 DAO) are associated with familial amyotrophic lateral sclerosis (FALS), and our preliminary
 data showed that the level of G72 autoantibody decreases in patients with ALS compared with
 normal control. Thus, we want to find out whether G72 plays a role in ALS and
 neurodegenerative diseases including Alzheimer disease and Parkinson's disease. First, we
 detect G72 protein and its autoantibody in sera of neurodegenerative diseases patients using
 ELISA and Western blotting, and the data are compared with normal control. We hypothesize the
 levels of G72 protein and its autoantibody in neurodegenerative diseases are less than those
 in normal control. Then, we extract genomic DNA of neurodegenerative diseases patients, and
 use polymerase chain reaction(PCR) to detect single nucleotide polymorphism (SNP) of G72. We
 aim to detect G72 missense SNP variants presented in ALS, AD and PD.
Inclusion Criteria:

 -  Clinical diagnosis of Alzheimer's Disease, Parkinson's disease and amyotrophic lateral
 sclerosis

 Exclusion Criteria:

 -