Stem Cell Study for Patients With Heart Disease
Stem Cell Study for Patients With Heart Disease
The purpose of this study is to determine if cell therapy with your own cells (autologous
 cells) delivered with a catheter to regions of the heart with poor blood flow will be safe
 and if it will relieve your chest pain and/or your ability to exercise.
The goal of this study is to determine the safety of various doses of autologous (one's own)
 stem cells, delivered with a catheter into the regions of the heart with poor blood flow.
 Stem cells are primitive cells produced by bone marrow that can develop into blood cells or
 other types of cells. In addition to determining whether this new approach is safe, the
 diagnostic tests may offer preliminary insights into the usefulness of this approach for
 treating myocardial ischemia (the condition where areas in the heart are lacking enough
 oxygen and blood flow to keep the heart muscle working well).

 This is a blinded, randomized study to compare a certain type of stem cell called
 CD34-positive versus a placebo agent (normal saline). You will have a 3:1 chance of receiving
 your CD34-positive stem cells versus the placebo agent (normal saline). You will not know
 whether you received the CD34-positive cells or the placebo agent (normal saline). If you are
 randomized to receive placebo (normal saline), you will undergo all of the pre-treatment
 phases of this study (including the stem cell mobilization phase and the apheresis
 procedure), but rather than receiving injections of CD34-positive cells, you will receive
 injections of the placebo agent (normal saline). There is some research evidence that
 suggests CD34-positive cells may help develop new blood vessels or improve blood flow when
 injected directly into the heart muscle.
Inclusion Criteria:

 -  Subjects with functional class (CCS) III or IV angina.

 -  Subjects who have attempted "best" medical therapy without control of symptoms.

 -  All subjects must have a recent coronary angiogram (within the last 3 months).

 -  Clinical signs and symptoms of clinically significant ischemia on nuclear perfusion
 imaging.

 -  Subjects must be able to complete a minimum of 1 minute but no more than 6 minutes of
 the Standard Bruce Protocol.

 -  Subject experiences angina during the baseline exercise tolerance test.

 -  Subjects must either be no longer capable of reproduction or taking acceptable
 measures to prevent reproduction during the study.

 -  Normal renal function.

 -  Normal liver function.

 -  Normal blood count.

 Angiographic Inclusions:

 -  Total occlusion of an epicardial coronary artery.

 -  Candidates at high risk for percutaneous coronary angioplasty of treatment zone(s)
 based upon clinical or anatomic considerations including but not limited to the
 following: diabetes, congestive heart failure (severe right heart failure, NYHA class
 III or IV), left main disease, pulmonary hypertension, severe proximal vessel
 tortuosity, severe bendpoint obstructions, diffuse disease (>2 cm in length), small
 vessel (<2 mm reference diameter), stenosis which are either diffuse (>2 cm in length)
 or distal, incessant restenosis lesions, unfavorable bifurcation stenosis, and
 degenerated or thrombosed saphenous vein grafts.

 Exclusion Criteria:

 -  Predominant congestive heart failure symptoms.

 -  Patients who have been hospitalized with a primary diagnosis of CHF in the prior 6
 months.

 -  Patients who have had diuretics added to their medical regimen or an increase in
 diuretic dosage for signs or symptoms of CHF in the past 6 months.

 -  Patients with a left ventricular ejection fraction of less than 25% as determined by
 transthoracic echocardiography.

 -  Patients with physical findings consistent with ongoing uncontrolled CHF.

 -  Myocardial infarction within 30 days of treatment.

 -  Successful coronary revascularization procedures within 3 months of study enrollment.

 -  Documented stroke or transient ischemic attack (TIA) within 60 days of study
 enrollment.

 -  History of severe aortic stenosis or insufficiency; severe mitral stenosis; or severe
 mitral insufficiency.

 -  Severe co-morbidity associated with a reduction in life expectancy of less than 1
 year.

 -  Subjects with PT, PTT or platelet counts greater than the upper limit of normal and
 those with a hematocrit <35%.

 -  Subjects with uncontrolled hypertension.

 -  Currently enrolled in another investigational device or drug trial (IDE or IND) that
 has not completed the required follow-up period.

 -  History of alcohol or drug abuse within 3 months of screening.

 -  Joint or peripheral vascular disease that severely limits treadmill walking.

 -  Chronic obstructive pulmonary disease that severely limits walking or FEV1.0<30%
 predicted.

 -  Subjects who are pregnant or lactating.

 -  Males and females who are capable of reproduction and will not take acceptable
 measures to prevent reproduction during the study.

 -  Subjects who test positive for HIV, hepatitis B or hepatitis C, have a chronic
 inflammatory disease, autoimmune disease or are on chronic immunosuppressive
 medications.

 -  Subjects with a known hypersensitivity to E. coli-derived proteins.

 -  Subjects with evidence (clinical, laboratory, or imaging) of cancer recurrence within
 the past 5 years (other than non-melanoma skin cancer or in situ cervical carcinoma).