Pharmacogenetics of b2-Agonists in Asthma.
Pharmacogenetics of b2-Agonists in Asthma.
This study will help to find out if having a certain genetic makeup influences how a person
 with asthma responds to salmeterol, one of the two drugs in Advair(R).
Patients are being asked to take part in this research study because they have asthma. This
 clinical research study is being done to see if an asthmatic's gene make-up (DNA is made up
 of genes) affects the way they respond to a particular asthma medication called salmeterol.
 Certain genes make people tall or short. Certain genes give people brown or black hair.
 Similarly, certain genes may be associated with the way patients respond to asthma
 medications.

 Salmeterol xinafoate (a long acting bronchodilator) and fluticasone propionate (an inhaled
 corticosteroid) are the medicines contained in Advair Diskus. During this study, patients
 with asthma will receive fluticasone inhaler (called Flovent) and Advair Diskus. The
 investigators want to find out if patients with asthma with certain genes respond in
 different ways to the salmeterol in Advair Diskus. The investigators also want to find out if
 patients with asthma with certain genes who are treated with salmeterol for two weeks have
 their airways open up less than usual when they use albuterol.
Inclusion Criteria:

 -  Diplotype: Whites with specific diplotype and African Americans with specific
 diplotypes.

 -  Gender: Male or female. Women are eligible if they are not pregnant or lactating.
 Females subjects of childbearing potential will undergo a urine pregnancy test prior
 to each methacholine challenge test.

 -  Age: 10 years and older.

 -  Asthma Diagnosis: Physician diagnosed asthma according to American Thoracic Society
 criteria for at least 3 months.

 -  Asthma Therapy: There is no requirement for previous asthma therapy to be included in
 this study.

 -  Asthma Severity: forced expiratory volume in the first second (FEV1) must be >= 60% of
 predicted normal values for age, height, and gender.

 -  methacholine challenge test provocative concentration (20% fall in FEV1) of
 <=12]mg/ml.

 Exclusion Criteria:

 -  History of life-threatening asthma: Any episode of asthma requiring intubation
 associated with hypercapnia, respiratory arrest, or hypoxic seizures.

 -  Asthma instability: Hospitalization for asthma within 3 months of Visit 1.

 -  Concurrent respiratory disease: Any respiratory disease other than asthma.

 -  Sensitivities: Sensitivities to methacholine, Flovent® MDI, ipratropium bromide,
 albuterol, or Advair Diskus® that would put the safety of the subject at risk.

 -  Respiratory Tract Infection: Any sinus, middle ear, oropharyngeal, upper or lower
 respiratory tract infection that has not resolved at least 2 weeks immediately
 preceding Visit 1, or for which antibiotic therapy has not been completed at least 2
 weeks prior to Visit 1.

 -  Expected exposure to pollen allergen to which the patient is sensitive (by medical
 history of symptoms) during the 29 day study period. These patients can be studied
 when pollen exposure to which they are sensitive will not occur.