Intra-articular Injection of Allogenic Lyophilized Growth Factors in Primary Knee Osteoarthritis
Intra-articular Injection of Allogenic Lyophilized Growth Factors in Primary Knee Osteoarthritis
the study was conducted on 31-patients with symptomatic primary knee osteoarthritis. The
 patients were randomized into intervention and control groups. The control group was kept on
 their medications without intervention. The intervention group received two doses of
 allogenic lyophilized growth factors (L-GFs); at baseline and after 2 months.
the study included 31 patients with symptomatic primary knee osteoarthritis (KOA) The study
 group was randomized into an intervention group and a control one. The control group patients
 were kept on their traditional medications without any intervention, while intervention group
 patients were instructed to stop using non-steroidal anti-inflammatory drugs (NSAID) at least
 1 week before the assessment and throughout the study. All participants were subjected to
 baseline clinical, radiographic, and ultrasonography assessment. Follow up assessments was
 conducted after 6 months.

 Patients of the intervention group received two doses of intra-articular knee injections of
 allogenic lyophilized growth factors (L-GFs); one dose at the baseline and the other was
 after 2 months.

 Lyophilized GFs were prepared at the Cairo Medical Centre Blood Bank from allogenic platelet
 rich plasma to according a previously published patented method.

 Prior to usage, reconstitution of the product was done using 1-ml saline and 1-ml lignocaine
 followed by gentle vial rubbing for 3 minutes. Then, the mixture was kept at ambient
 temperature for 5 minutes to ensure complete protein re-hydration.

 The injection was done under complete guidance of ultrasonography through lateral
 supra-patellar short axis in-plane approach under sterile aseptic conditions. After
 injection, local ice application and oral paracetamol were allowed if the patient felt pain.
 NSAIDs were completely avoided as they may inhibit the desired post-injection inflammatory
 process. Patients were encouraged to restrict vigorous knee activities for 48 hours.
Inclusion Criteria

 -  Clinical and radiographic diagnosis of primary knee osteoarthritis

 -  Patients must be symptomatic

 Exclusion Criteria:

 -  Patients with secondary knee osteoarthritis

 -  previous intra-articular steroid, or hyaluronic knee injection in last year

 -  Patients with other disabling lower limb osteoarthropathy or myopathy