Circulating Cell-free DNA in Metastatic Melanoma Patient: Mutational Analyses in Consecutive Measurement Before and After Chemotherapy
Circulating Cell-free DNA in Metastatic Melanoma Patient: Mutational Analyses in Consecutive Measurement Before and After Chemotherapy
Therapies that target specific molecules markedly inhibit cancer growth in several
 malignancies, and provide valuable strategies for the treatment of advanced melanoma. In
 recent years, BRAF and KIT have become established therapeutic targets in melanoma patients
 showing activating mutations in these oncogenes. However, it is crucial that genetic
 mutations present in the melanoma lesions are identified if the investigators are to design
 tailormade therapies for individual patients. The tumour genotypes that determine the
 selection of molecular-targeted therapies are usually identified in primary tumours; however,
 primary tumours are not always representative of metastases. Circulating free DNA may be a
 source of valuable information because it can be obtained via routine blood sampling, it
 provides real-time information about a patient's current disease state, and it allows
 monitoring and molecular characterization before and after chemotherapy. The aim of the study
 is to determine the mutational status in circulating DNA in melanoma metastatic patients,
 with the Sequenom Mass Array, a next generation sequencing technology. Results obtained
 before and after treatment will be compared with the primary tumor genotype.
Inclusion Criteria:

 -  Age>18 years

 -  Surgical biopsy for histologic diagnostic

 -  All melanoma subtypes

 -  Known genotype BRAF V600

 -  Affiliation social security

 -  Consent form signed

 Exclusion Criteria:

 -  Patient with histories of cancer or the other synchronous cancer

 -  Pregnant women

 -  Breast-feeding women

 -  Vulnerable patients: major under guardianship; patient deprived of its rights