Intake of Beta-glucan and Postprandial Regulation of Blood Glucose Metabolism in Healthy Subjects
Intake of Beta-glucan and Postprandial Regulation of Blood Glucose Metabolism in Healthy Subjects
The overall aim is to investigate the intake of beta-glucan in relation to glucose metabolism
 and satiety in a postprandial study with healthy subjects. The potential effects will be
 related to changes in the gut microbiota, the circulating levels of short chain fatty acids,
 inflammation and gene expression in peripheral mononuclear blood cells
The intervention study will have a fixed order, cross-over design with three test meals
 containing low (0.5 g / 100 g of product), medium (3.5 g / 100 g of product) and high (8 g /
 100 g product) amount of beta -glucans, respectively. The test meals are in the form of
 cereals. All participants will eat the three test meals three constitutive days with 2 weeks
 apart. At day four, the participants will perform a postprandial glucose test (OGTT, 75 g
 glucose in 150 ml water) at Oslo and Akershus University College. Blood samples will be taken
 before and at different time points after glucose test.

 At the screening visit the participants will be asked to limit the intake of dietary fiber
 from grains two weeks prior to the baseline visit (0) and during the study. Otherwise,
 participants will be asked not to change their diet and exercise habits during the study
 period.

 At the baseline visit (visit 0) an OGTT will be performed. OGTT will also be performed at
 visit 1, 3 and 5 after intake of low, medium and high beta-glucan, respectively.

 The participants will receive the test meals at visit 1, 2 and 4.
Inclusion Criteria:

 -  BMI between 18,5 and 27 kg/m2

 -  Fasting plasma glucose ≤ 6.1 mmol/l

 Exclusion Criteria:

 -  Chronic metabolic diseases such as diabetes type 1 and 2, coronary heart disease and
 cancer the last 6 months.

 -  Intestinal diseases such as chrons disease, ulcerative colitis and irritable bowel
 syndrome.

 -  Food allergy and intolerances towards grain and dairy products.

 -  Pregnant and lactating

 -  Smokers

 -  Fasting blood glucose ≥ 6.1 mmol/L

 -  CRP > 10 mg/L, measured at baseline (visit 0)

 -  BMI <18,5 and >27 kg/m2

 -  Planned weight reduction and or ± 5% weight change over the past three months.

 -  Use of antibiotics last 3 months before study entry and during the study period

 -  Use of probiotics the last month before study entry and during the study period

 -  Blood donor last 2 months before study entry and or during the study period

 -  Not willing to end the use of dietary supplements four weeks prior to study entry and
 throughout the study period

 -  Alcohol consumption > 40g / day

 -  Hormone treatments (except contraceptives)