BioImpedentiometry, Lung UltraSound and cONgestion in Heart Failure
BioImpedentiometry, Lung UltraSound and cONgestion in Heart Failure
The purpose of this study is to validate non-invasive and user-friendly methods to monitor
 systemic and pulmonary congestion in heart failure patients.

 The primary objective is to validate the role of bioimpedentiometry, pulmonary and
 subcutaneous ultrasound, to assess changes in total body water in patients with heart
 failure. vs the gold standard technique of deuterium oxide dilution Secondary objectives are

 - to evaluate the applicability of bioimpedentiometry, pulmonary and subcutaneous ultrasound
 to monitor systemic, pulmonary and peripheral district congestion in relation with clinical
 and laboratory variables; 2)to analyze the organizational issues related to the use of these
 methods.
Increased congestion leads to the clinical manifestation of heart failure (dyspnea,
 peripheral edema) and is considered the leading cause of hospital admissions and death among
 patients with HF Preclinical recognition of congestion in HF patients through home monitoring
 is crucial to prevent acute exacerbations and hospital admissions. Daily body weight, vital
 signs and fluid balance give only an indirect assessment of the level of congestion.

 Deuterium oxide dilution is the non-invasive reference method for total body water
 assessment.

 Bioelectrical impedance analysis (BIA) and chest ultrasound are simple, non-invasive
 techniques potentially applicable for home monitoring of congestion by non-physicians.

 BIA is a noninvasive method for the study of body composition based on the measurement of
 body electrical resistance; BIA may allow measurement of whole body and segmental, e.g.
 pulmonary, fluids. However data on the value of BIA for the monitoring of HF patients are
 still scarce, inconclusive and relative to small cohorts of patients.

 Lung ultrasound (LUS) through evaluation of B-lines, reverberation artifacts in the presence
 of extravascular lung water has been proposed as a semiquantitative tool to assess pulmonary
 congestion. B lines showed a good correlation with multiple indicators of congestion.

 The aim of this study is to validate whole body and segmental BIA and lung ultrasound vs the
 gold standard deuterium dilution technique to monitor changes in systemic and pulmonary
 congestion after treatment in the setting of acute heart failure (AHF Group 1) and advanced
 chronic heart failure (ad-CHF Group 3) using as controls stable chronic heart failure
 outpatients (CHF Group 2).

 Enrolled patients will simultaneously undergo clinical assessment of congestion (weight gain,
 pulmonary rales, jugular venous pressure, peripheral edema, chest X-ray), routine laboratory,
 BIA and pulmonary and subcutaneous ultrasound and deuterium administration as follows Group 1
 (Acute Heart Failure - AHF) Patients will be assessed within 24 hours of admission for acute
 heart failure (T0), and at hospital discharge after an average of 10 days (Td).

 Group 2 (Chronic Heart Failure - CHF) Patients will be assessed during a programmed
 outpatient visit (T0) and after 10 ± 3 days (T1).

 Group 3 (Advanced Chronic Heart Failure -Ad-CHF) Ad-CHF patients on periodic levosimendan
 undergo monthly levosimendan infusions in the outpatient clinic. Baseline assessments will be
 performed the day of levosimendan administration, before infusion start(T0) and after 80 +/-
 12 hours, i.e at the peak haemodynamic effect of levosimendan (Tp).

 In all patient groups, vital status and hospital admissions or emergency department visits
 and their cause will be assessed after 6 months by telephone follow-up.
Inclusion Criteria:

 -  Signed informed consent

 Group-specific inclusion criteria:

 1. Group 1 (Acute Heart Failure - AHF), patient admitted to the hospital for acute heart
 failure (de-novo or exacerbation of chronic heart failure), who have at least two of
 the following congestion criteria:

 -  weight gain ≥ 2 kg over the three days prior to hospitalization

 -  physical findings or radiographic signs of pulmonary congestion (crackles,
 semi-orthopnea)

 -  Hepatomegaly > 2 cm from the arch rib or ascites

 -  jugular turgor or central venous pressure > 10 cm H20

 -  Ankle swelling

 2. Group 2 (Chronic Heart Failure - CHF): outpatients with chronic heart failure (CHF)
 with characters of clinical stability

 -  No hospitalization in the previous six months

 -  Symptoms stable for at least 4 weeks

 -  Oral therapy stable for at least 4 weeks

 -  No involuntary weight variations greater than 2 kg in the last 4 weeks

 -  Absence of signs of congestion

 3. Group 3 (advanced Chronic Heart Failure - ad-CHF) Patients on optimal medical therapy
 for HF and treated with periodic infusions of levosimendan (every 4 weeks) according
 to the following criteria

 -  Systolic dysfunction (LVEF <35%)

 -  NYHA Class IIIb-IV and/or level INTERMACS 4, 5, 6

 -  ≥2 admissions or access to the emergency department for acute HF exacerbation in
 the previous 12 months

 Exclusion Criteria:

 -  Uremia in dialysis treatment

 -  Invasive mechanical ventilation

 -  Mechanical circulatory support

 -  Cardiac cachexia defined as weight loss ≥ 5% in the prior 12 months or body mass index
 <20 kg/m2 and hypoalbuminemia (albumin <3.2 g/dl) or anemia (hemoglobin <12 g/l)