Expression of Toll-Like Receptors 2 and 4 in Asthmatic Patients.
Expression of Toll-Like Receptors 2 and 4 in Asthmatic Patients.
Introduction: Recently it has involved the role of TLR (toll-like receptors) in the
 pathogenesis of asthma. TLRs are a family of proteins responsible for the recognition of
 Pathogen-Associated Molecular Patters (PAMPs). The possible role that TLRs play in
 IgE-dependent asthma (not eosinophilic) and its recognition as a new endotype asthma, could
 open new therapeutic expectations.

 Objective: To analyze the expression of TLR 2 and 4 in monocytes/ macrophages and neutrophils
 in peripheral blood and induced sputum from asthmatic patients
The investigators studied patients with asthma. All patients underwent the same day an
 induced sputum, pulmonary function studies, fractional exhaled nitric oxide, blood total IgE
 and skin test by prick technique. Asthmatic patients were classified according to the
 criteria of the GINA 2012. The cellular composition was analyzed by flow cytometry.
 Expression of TLR2 and TLR4 were analyzed using monoclonal antibodies conjugated with
 fluorochromes appropriate.
Inclusion Criteria:

 -  Adults with persistent asthma (defined as per the Global INitiative for Asthma
 Management (GINA) criteria)

 Exclusion Criteria:

 -  If they had a respiratory tract infection or asthma exacerbation within 30 days prior
 to inclusion

 -  If they were receiving oral steroids or immunosuppressive treatments.