Evaluating the Safety and Drug Levels of an Antibody Against HIV in Healthy, HIV-Uninfected Adults
Evaluating the Safety and Drug Levels of an Antibody Against HIV in Healthy, HIV-Uninfected Adults
Antibodies are natural proteins that the body makes to fight infections. Antibodies can also
 be manufactured like a drug and infused or injected into the body to prevent or treat a
 disease. The purpose of this study is to test the safety of and the body's response to an
 antibody against HIV in healthy, HIV-uninfected adults.
The purpose of this study is to evaluate the safety, tolerability, and drug levels of five
 different schedules for the intravenous (IV) and subcutaneous (SC) administration of a human
 monoclonal antibody (VRC-HIVMAB060-00-AB [VRC01]) against HIV in healthy, HIV-uninfected
 adults.

 The study will enroll 88 healthy, HIV-uninfected adults aged 18 to 50 years.

 This study will enroll participants in 5 groups. Groups 1-3 will enroll simultaneously.
 Groups 1 and 2 will be randomized together but not blinded, while Group 3 will be randomized
 separately and will be blinded. With the implementation of Version 2.0, Groups 4 and 5 will
 be randomized together and will enroll simultaneously. Each group will have a different
 schedule of clinic visits and receive different doses of VRC01 or placebo for VRC01.
 Participants will attend 8 months of scheduled clinic visits.

 Participants in Group 1 will receive an IV infusion of VRC01 on Days 0, 28, 56, 84, 112, and
 140. Participants in Group 2 will receive an IV infusion of VRC01 on Days 0, 56, and 112.
 Participants in Group 3 will receive an IV infusion of VRC01 or IV placebo for VRC01 on Day
 0, followed by SC injections of VRC01 or SC placebo for VRC01 on Days 14, 28, 42, 56, 70, 84,
 98, 112, 126, 140, and 154. Participants in Groups 4 and 5 will receive an IV infusion of
 VRC01 (each group will receive a different dose) on Days 0, 56, and 112.

 Participants will remain in the clinic for about an hour after receiving the infusions and
 injections for observation and monitoring. Participants in Group 3 who have no problems with
 the first SC injection will have to wait in the clinic for only a half an hour after the rest
 of the SC injections.

 At study entry, participants will give a medical history; undergo a physical exam, blood
 collection, and urine collection; and receive HIV risk reduction counseling. At follow-up
 visits, participants will undergo a brief physical exam and blood collection, receive HIV
 risk reduction counseling, and be asked questions about their health and their experience
 participating in the study. At all visits, participants who were born female will have a
 pregnancy test. At select study visits, saliva, rectal, and semen or cervical secretion
 samples will be collected from participants who consent to collection of these samples.
Inclusion Criteria:

 General and Demographic Criteria

 -  Weight at least 53 kg and up to 115 kg

 -  Access to a participating HIV Vaccine Trials Network (HVTN) clinical research site
 (CRS) and willingness to be followed for the planned duration of the study

 -  Ability and willingness to provide informed consent

 -  Assessment of understanding: participant demonstrates understanding of this study;
 completes a questionnaire prior to first infusion, with verbal demonstration of
 understanding of all questionnaire items answered incorrectly

 -  Agrees not to enroll in another study of an investigational research agent until
 completion of the last study visit

 -  Good general health as shown by medical history, physical exam, and screening
 laboratory tests

 HIV-Related Criteria

 -  Willingness to receive HIV test results

 -  Willingness to discuss HIV infection risks, amenable to HIV risk reduction counseling,
 and committed to maintaining behavior consistent with low risk of HIV exposure through
 the last required protocol clinic visit

 -  Assessed by the clinic staff as being at "low risk" for HIV infection and committed to
 maintaining behavior consistent with low risk of HIV exposure through the last
 required protocol clinic visit

 Laboratory Inclusion Values

 Hemogram/Complete Blood Count (CBC)

 -  Hemoglobin greater than or equal to 11.0 g/dL for participants who were born female,
 greater than or equal to 13.0 g/dL for participants who were born male

 -  White blood cell count equal to 2,500 to 12,000 cells/mm^3

 -  Total lymphocyte count greater than or equal to 800 cells/mm^3

 -  Remaining differential either within institutional normal range or with site physician
 approval

 -  Platelets equal to 125,000 to 550,000/mm^3

 Chemistry

 -  Chemistry panel: alanine aminotransferase (ALT), aspartate aminotransferase (AST), and
 alkaline phosphatase less than 1.25 times the institutional upper limit of normal;
 creatinine less than or equal to the institutional upper limit of normal

 Virology

 -  Negative HIV-1 and -2 blood test: U.S. participants must have a negative Food and Drug
 Administration (FDA)-approved enzyme immunoassay (EIA).

 -  Negative hepatitis B surface antigen (HBsAg)

 -  Negative anti-hepatitis C virus (anti-HCV) antibodies, or negative HCV polymerase
 chain reaction (PCR) if the anti-HCV is positive

 Urine

 -  Normal urine:

 -  Negative urine glucose, and

 -  Negative or trace urine protein, and

 -  Negative or trace urine hemoglobin (if trace hemoglobin is present on dipstick, a
 microscopic urinalysis with red blood cell levels within institutional normal
 range).

 Reproductive Status

 -  Participants who were born female: negative serum or urine beta human chorionic
 gonadotropin (beta-HCG) pregnancy test performed prior to infusion on the day of
 initial infusion. Persons who are not of reproductive potential because of having
 undergone total hysterectomy with bilateral oophorectomy (verified by medical records)
 are not required to undergo pregnancy testing.

 -  Reproductive status: A participant who was born female must:

 -  Agree to consistently use effective contraception (see the protocol for more
 information) for sexual activity that could lead to pregnancy from at least 21
 days prior to enrollment through the last required protocol clinic visit.
 Effective contraception is defined as using any of the following methods: condoms
 (male or female) with or without a spermicide; diaphragm or cervical cap with
 spermicide; intrauterine device (IUD); hormonal contraception; any other
 contraceptive method approved by the HVTN 104 Protocol Safety Review Team (PSRT);
 successful vasectomy in the male partner (considered successful if a participant
 reports that a male partner has [1] documentation of azoospermia by microscopy,
 or [2] a vasectomy more than 2 years ago with no resultant pregnancy despite
 sexual activity postvasectomy);

 -  Or not be of reproductive potential, such as having reached menopause (no menses
 for 1 year) or having undergone hysterectomy, bilateral oophorectomy, or tubal
 ligation;

 -  Or be sexually abstinent.

 -  Participants who were born female must also agree not to seek pregnancy through
 alternative methods, such as artificial insemination or in vitro fertilization until
 after the last required protocol clinic visit.

 Exclusion Criteria:

 General

 -  Blood products received within 120 days before first infusion, unless eligibility for
 earlier enrollment is determined by the HVTN 104 PSRT

 -  Investigational research agents received within 30 days before first infusion

 -  Intent to participate in another study of an investigational research agent during the
 planned duration of the HVTN 104 study

 -  Pregnant or breastfeeding

 Vaccines and Other Injections

 -  HIV vaccine(s) received in a prior HIV vaccine trial. For participants who have
 received control/placebo in an HIV vaccine trial, the HVTN 104 PSRT will determine
 eligibility on a case-by-case basis.

 -  Non-HIV experimental vaccine(s) received within the last 6 months in a prior vaccine
 trial. Exceptions may be made for some vaccines and vaccine trials. For participants
 who have received an experimental vaccine(s) less than 6 months ago, eligibility for
 enrollment will be determined by the HVTN 104 PSRT on a case-by-case basis.

 -  Live attenuated vaccines other than influenza vaccine received within 10 days before
 first infusion and with no evidence of residual inflammation; or scheduled within 10
 days after first infusion (e.g., measles, mumps, and rubella [MMR]; oral polio vaccine
 [OPV]; varicella; yellow fever).

 -  Previous receipt of humanized or human monoclonal antibodies (mAbs) whether licensed
 or investigational

 Immune System

 -  Immunosuppressive medications received within 30 days before first infusion. (Not
 excluded: [1] corticosteroid nasal spray; [2] inhaled corticosteroids; [3] topical
 corticosteroids for mild, uncomplicated dermatitis; or [4] a single course of
 oral/parenteral corticosteroids at doses less than 2 mg/kg/day and length of therapy
 less than 11 days, with completion at least 30 days prior to enrollment.

 -  Serious adverse reactions to vaccines or to vaccine components, including history of
 anaphylaxis and related symptoms such as hives, respiratory difficulty, angioedema,
 and/or abdominal pain. (Not excluded: a participant who had a nonanaphylactic adverse
 reaction to pertussis vaccine as a child)

 -  Immunoglobulin received within 90 days before first infusion, unless eligibility for
 earlier enrollment is determined by the HVTN 104 PSRT.

 -  Autoimmune disease (Not excluded: participant with mild, stable, and uncomplicated
 autoimmune disease that does not require immunosuppressive medication and that, in the
 judgment of the site investigator, is likely not subject to exacerbation and likely
 not to complicate reactogenicity and adverse event [AE] assessments)

 -  Immunodeficiency

 Clinically Significant Medical Conditions

 -  Untreated or incompletely treated syphilis infection

 -  Clinically significant medical condition, physical examination findings, clinically
 significant abnormal laboratory results, or past medical history with clinically
 significant implications for current health. A clinically significant condition or
 process includes but is not limited to:

 -  A process that would affect the immune response,

 -  A process that would require medication that affects the immune response,

 -  Any contraindication to repeated infusions or blood draws,

 -  A condition that requires active medical intervention or monitoring to avert
 grave danger to the participant's health or well-being during the study period,

 -  A condition or process for which signs or symptoms could be confused with
 reactions to vaccine, or

 -  Any condition specifically listed among the exclusion criteria below.

 -  Any medical, psychiatric, occupational, or other condition that, in the judgment of
 the investigator, would interfere with, or serve as a contraindication to, protocol
 adherence, assessment of safety or reactogenicity, or a participant's ability to give
 informed consent

 -  Psychiatric condition that precludes compliance with the protocol. Specifically
 excluded are persons with psychoses within the past 3 years, ongoing risk for suicide,
 or history of suicide attempt or gesture within the past 3 years.

 -  Current anti-tuberculosis (TB) prophylaxis or therapy

 -  Asthma other than mild, well-controlled asthma (symptoms of asthma severity as defined
 in the most recent National Asthma Education and Prevention Program [NAEPP] Expert
 Panel report).

 -  Exclude a participant who uses a short-acting rescue inhaler (typically a beta 2
 agonist) daily, or uses moderate/high dose inhaled corticosteroids, or in the past
 year has either of the following:

 -  Greater than one exacerbation of symptoms treated with oral/parenteral
 corticosteroids;

 -  Needed emergency care, urgent care, hospitalization, or intubation for asthma.

 -  Diabetes mellitus type 1 or type 2, including cases controlled with diet alone. (Not
 excluded: history of isolated gestational diabetes)

 -  Hypertension:

 -  If a person has been found to have elevated blood pressure or hypertension during
 screening or previously, exclude for blood pressure that is not well controlled.
 Well controlled blood pressure is defined as consistently less than or equal to
 140 mm Hg systolic and less than or equal to 90 mm Hg diastolic, with or without
 medication, with only isolated, brief instances of higher readings, which must be
 less than or equal to 150 mm Hg systolic and less than or equal to 100 mm Hg
 diastolic. For these participants, blood pressure must be less than or equal to
 140 mm Hg systolic and less than or equal to 90 mm Hg diastolic at enrollment.

 -  If a person has NOT been found to have elevated blood pressure or hypertension
 during screening or previously, exclude for systolic blood pressure greater than
 or equal to 150 mm Hg at enrollment or diastolic blood pressure greater than or
 equal to 100 mm Hg at enrollment.

 -  Bleeding disorder diagnosed by a doctor (e.g., factor deficiency, coagulopathy, or
 platelet disorder requiring special precautions)

 -  Malignancy (Not excluded: participant who has had malignancy excised surgically and
 who, in the investigator's estimation, has a reasonable assurance of sustained cure,
 or who is unlikely to experience recurrence of malignancy during the period of the
 study)

 -  Seizure disorder: history of seizure(s) within past 3 years. Also exclude if
 participant has used medications in order to prevent or treat seizure(s) at any time
 within the past 3 years.

 -  Asplenia: any condition resulting in the absence of a functional spleen

 -  History of hereditary angioedema, acquired angioedema, or idiopathic angioedema.