Dynamic Computed Tomography Myocardial Perfusion Imaging for Detection of Coronary Artery Disease
Dynamic Computed Tomography Myocardial Perfusion Imaging for Detection of Coronary Artery Disease
Coronary artery computed tomographic angiography (CTA) is a widely used, highly accurate
 technique for the detection of coronary artery disease (CAD), with sensitivity and negative
 predictive values of over 90% (1-4). Patients with normal CTA findings have an excellent
 prognosis and do not require further testing for CAD (5). However, like invasive coronary
 angiography (QCA), CTA is an anatomic test and, unless lesions are very severe (>90%
 stenosis), cannot reliably predict the impairment of flow (functional significance) of
 intermediate grade stenoses.

 For this reason, in approximately 15-25% of patients, additional functional testing may be
 required after CTA, usually in the form of stress testing (6-8). Stress testing is commonly
 done by exercise or pharmacologic stress with electrocardiographic monitoring and often,
 imaging of myocardial perfusion by nuclear scintigraphy (MPI) or detection of abnormal
 contraction by echocardiography. This requires a separate procedure, entailing time, expense
 and limited risk. Furthermore, in patients with previously known CAD, CTA alone is not an
 adequate test, because in most cases there are multiple lesions that are possible sources of
 ischemia.

 Over the last 10 years, these investigators and others around the world have developed a
 method of imaging myocardial perfusion by CT (CTP). This test is an adjunct to the usual
 Cardiac Computed Tomography Angiography (CCTA) procedure and can be done immediately
 thereafter, using conventional pharmacologic stress agents. It has demonstrated accuracy in
 many single center trials, and in this large multicenter study, the CORE320 trial (9,10)
 which showed a high accuracy in predicting the combined results of QCA plus MPI testing and a
 second multicenter trial established non-inferiority of myocardial CTP compared with nuclear
 stress testing (11,12). Additionally, this investigator group has published a direct
 comparison of diagnostic performance of myocardial CTP imaging and SPECT myocardial perfusion
 imaging and demonstrated superior diagnostic performance of CTP imaging compared with SPECT
 for the diagnosis of significant disease on invasive angiography (13).

 CTP images can be acquired with two different approaches: static or dynamic. In the CORE320
 study, the CTP protocol used static acquisition method. The static CTP method, samples a
 snapshot of the iodine distribution in the blood pool and the myocardium over a short period
 of time, targeting either the upslope or the peak of contrast bolus. The notion behind this
 is that, at the upslope of the contrast, the difference in attenuation value of the ischemic
 and remote myocardium is at the maximum which enables for qualitative and semi-quantitative
 assessment of myocardial perfusion defects. The static CTP, however, does not allow for
 direct quantification of the myocardial blood flow (MBF). One of the drawbacks of static CTP
 lies in the acquirement of only one sample of data and the possibility of mistiming of the
 contrast bolus that results in poor contrast-to-tissue ratios by missing the peak attenuation
 (14). Output and flow rate of the contrast material may affect bolus timing. In addition, the
 acquisition of data from sequential heartbeats affects the attenuation gradient and may
 result in a heterogeneous iodine distribution, mimicking perfusion defects (15). Furthermore,
 the static CTP is limited in detection of balanced ischemia, where the perfusion of the
 entire myocardium is impaired and therefore there is no reference remote myocardium for
 comparison for semi-quantitative or qualitative static methods of CTP interpretation.

 Dynamic CT perfusion imaging uses serial imaging over time to record the kinetics of
 iodinated contrast in the arterial blood pool and myocardium. This technique allows for
 multiple sampling of the myocardium and the blood pool and creating time attenuation curves
 (TAC) by measuring the change in CT attenuation over time. Mathematical modelling of TACs
 permits for direct quantification of MBF. Despite its advantages, the use of dynamic CTP were
 limited in the past. A high temporal resolution and high number of detectors are required for
 dynamic CTP to allow for entire myocardial coverage, and in order to obtain multiple
 consecutive images at high heart rates(16,17). But the main challenge of dynamic CTP
 acquisition was the high radiation dose associated with this technique. Nevertheless, with
 the introduction of the cutting-edge 320 detector CT scanning systems with fast gantry
 rotation the issue of the cardiac coverage is eliminated(17). The second-generation 320-row
 scanners also permit the quantification of the MBF with dynamic CTP acquisition with
 relatively low-dose of radiation(18,19).

 In this study the investigators aim to evaluate the feasibility, safety and accuracy of the
 low-radiation dose dynamic myocardial CT perfusion compared to static CTP approach to detect
 hemodynamically significant coronary artery disease.
This will be a prospective study comparing the low-dose dynamic vs. static CTP combined with
 the CTA for detecting hemodynamically significant coronary artery stenosis. The aim of the
 study is to assess the feasibility, safety and accuracy of low-dose dynamic CTP following
 CTA.

 The study will enroll patients who have documented coronary artery disease and an indication
 for coronary angiography or CT angiography. Referred patients will be assessed for
 eligibility through phone calls followed by in-person interviews. Patents will be provided
 with the informed consent forms for CTA-CTP and iodine contrast if it is determined that
 participants are eligible for the study. Patients have the right to refuse to participate in
 the study and in that case participants will receive the regular care, per clinical
 guidelines. Baseline information will be collected from the patients after participants
 consent to participate in the study. Baseline data collection will happen at the same day
 that the patients will undergo CTA-CTP. The CTA-CTP acquisition takes less than 60 minutes
 after the patient is on the CT scanner table. Patient preparation time before patient is
 brought on the CT scanner depends on patients' heart rate and the time that takes to reduce
 patient's heart rate to a level that is appropriate for CTA-CTP acquisition. The blood sample
 will be obtained from the patients at the same day as CTA-CTP acquisition. Patients will be
 discharged to home at the same day participants undergo CTA-CTP.

 b. Study duration and number of study visits required of research participants. There will be
 only one visit required for the purposes of the study, during which the CTA-CTP acquisition
 will be completed, baseline information will be collected and the blood samples will be
 drawn. The study participants will be contacted through phone 3-days after CTA-CTP
 acquisition for follow-up.

 CT Imaging protocol

 Patients will have two 18-20 gauge intravenous lines placed, one preferably in an antecubital
 vein for contrast administration. The patient will be hydrated with normal saline
 intravenously (250 - 500 ml) prior to CT scanning. The patient will lie supine on the scanner
 table and be attached to a 12 lead electrocardiographic monitor and an automated blood
 pressure monitor. Baseline ECG, heart rate, and blood pressure will be recorded and reviewed
 by one of the study investigators. Due to resultant artifacts from precordial leads, the 12
 ECG leads and electrodes will be removed and rhythm monitoring will continue using the 3 lead
 system attached to the scanners monitoring system during scanning. A physician will be
 present at all times during adenosine infusion and CT imaging. Patients may receive an oral
 and/or intravenous dose of metoprolol up to one hour prior to the CT. If the heart rate is
 >60 beats per minute, 75 mg (max 80 mg) of metoprolol will be given orally. If Heart rate
 remains >60 beats per minute at the scan acquisition then, intravenous beta blocker
 (metoprolol, propranolol or landiolol) 2.5 - 5.0 mg every 5 minutes will be administered to
 achieve a heart rate between <60 beats per minute as blood pressure tolerates under the
 supervision of a physician. Scout images for determining scanning range will be obtained in
 the anterior-posterior and lateral views. Patients with systolic blood pressure ≥110 will
 receive sublingual fast acting short lasting nitrates (e.g. nitroglycerin, isosorbide
 dinitrate). Patients will then be asked to hold their breath (approximately 10-15 seconds)
 and non-contrast CT imaging will be performed starting just cranial to the coronary ostia and
 extending just caudal to the apex of the heart in order to obtain a coronary calcium score.
 CT angiogram will be performed to evaluate the coronaries and myocardial perfusion at rest.
 Blood pressure will be checked and intravenous adenosine infusion (0.14 mg/kg/min) will begin
 with continuous heart rate and rhythm monitoring. After 5 minutes of adenosine infusion,
 contrast-enhanced CT perfusion imaging will be performed during a 4-5 ml/sec intravenous
 iodinated contrast (ISOVUE®-370) infusion. Total contrast dose for the entire protocol will
 not exceed 140 ml and will be based on patient's body size. Patients will be asked to hold
 their breath during scanning. Immediately following completion of the scan, the adenosine
 infusion will be discontinued. A twelve lead ECG and blood pressure measurement will be
 repeated after discontinuation of adenosine and reviewed by a physician. Intravenous
 hydration will be continued during recovery with normal saline for a total volume for the
 entire post scan of 250 to 500 ml if deemed appropriate by the supervising physician.

 320-Detector CT Protocol for Combined Coronary Angiography and Perfusion Imaging

 1. Coronary calcium scan will be performed using the following protocol:

 -  No contrast.

 -  CT Imaging: tube voltage = 120kV, tube current = 140 mA, gantry rotation speed =
 0.35 seconds, slice thickness = 0.5 mm, rows = 256-320, range = 128-160 mm. X-ray
 tube will be on for a total of 0.35 seconds. Estimated radiation dose = 1.5 mSv.

 2. Rest coronary arterial imaging Rest perfusion and coronary arterial imaging will be
 performed during a 4-5 ml/sec intravenous iodinated contrast (ISOVUE®-370) infusion. The
 rest CTA-dynamic CTP images will be started using a test bolus acquisition and accurate
 quantification of optimum timing for dynamic CTP and boost CTA acquisition and will
 continue for 20-30 sec using a ECG triggering method to allow acquiring the images only
 within 70-80% of the R-R interval, but not continuously. The parameters for the dynamic
 CTP image acquisition are the followings: For the heart rate of <60 bpm the tube voltage
 will be 80 kV and the tube current will be 100mA. Other parameters are: gantry
 rotation=0.275, range=120mm. The CTA and static CTP imaging will be performed as a boost
 scan during the dynamic CTP with the same tube voltage (80kV) but the tube current of
 600mA within 70-80% of R-R interval. The boost timing will be quantified from test bolus
 acquisition. The average radiation dose for rest CTA and rest dynamic and static CTP
 acquisition is 3.69mSv.

 3. Stress Myocardial Perfusion imaging 20 minutes after rest image acquisition

 Blood pressure will be checked and intravenous adenosine infusion will begin with continuous
 heart rate and rhythm monitoring. After 5 minutes of adenosine infusion, contrast-enhanced CT
 perfusion imaging will be performed during a 4-5 ml/sec intravenous iodinated contrast
 (ISOVUE®-370) infusion. The stress dynamic CTP images will be started using a test bolus
 acquisition and accurate quantification of optimum timing for dynamic CTP and boost CTA
 acquisition and will continue for 20-30 sec using a ECG triggering method to allow acquiring
 the images only within 70-80% of the R-R interval, but not continuously. The parameters for
 the stress dynamic CTP image acquisition are the followings: For the heart rate of <80 bpm
 the tube voltage will be 80 kV and the tube current will be 100mA. Other parameters are:
 gantry rotation=0.275, range=120mm. The stress static CTP imaging will be performed as a
 boost scan during the stress dynamic CTP with the same tube voltage (80kV) but the tube
 current of 600mA within 70-80% of R-R interval. The boost timing will be quantified from test
 bolus acquisition. The average radiation dose for stress dynamic and static CTP acquisition
 is 5.17mSv. The scan parameters are summarized in the table below:

 HR kV mA Rot Range Cardiac Phase Total Scan Time Boost Skip beat CTDIVol DLP Dose [mSv] Dose
 Sum [mSv] CAC score 120 140 0.35 120 70-80 0.35 1.5 10.551 Test Bolus every 2s 33.8 13 0.182
 CTA-CTP Rest 60 80 100 0.275 120 70-80 25 V 600mA 22 264.1 3.6974 Stress 80 80 100 0.275 120
 70-80 25 V 600mA 30.8 369.4 5.1716

 The estimated average radiation dose for the entire cardiac computed protocol is 10.55 mSv
 and shall not exceed 15mSv. Beta-blockers will be used to control the heart rate and thus
 maintain the radiation dose as low as reasonably achievable. The total contrast dose will not
 exceed 140 ml. Depending on patient size, 50-70 ml of iodinated contrast will be used for
 each of rest and stress scans.

 In the event that the CT raw data are not readable at the Johns Hopkins University, a copy of
 the raw data (anonymized without patient identifiers) will be transferred to Toshiba Medical
 Systems for engineering support of image reconstruction in these isolated cases. Toshiba
 Medical Systems will perform the image reconstruction and return the raw data and the
 reconstructed image data to the Johns Hopkins University for image analysis.

 Within 3 weeks of the CT, the CT will be reviewed for non-cardiac findings by a locally
 qualified, institutionally approved cardiologist and reported to the patient's clinical
 physician and patient in a timely fashion preferably prior to or during the 30-day follow-up.

 c. Blinding, including justification for blinding or not blinding the trial, if applicable.

 Dynamic and static CTP images will be analyzed separately and when analyzing the CTP images
 with each method, the readers will be blinded to the results the other method. However the
 readers will have access to the results for CTA interpretation while reading the CTP images
 with either of static or dynamic methods.

 d. Justification of why participants will not receive routine care or will have current
 therapy stopped.

 The study will be only enrolling patients with an indication for CTA or invasive coronary
 angiography and will undergo these tests. So routine care will be delivered to the
 participants.

 7. Study Statistics

 a. Primary outcome variable. The primary outcome measures will be feasibility, safety and
 accuracy of dynamic CTP-CTA compared to static CTP-CTA to detect hemodynamically significant
 coronary artery stenosis. The hemodynamically significant coronary artery stenosis will be
 defined as having at least one vessel with a ≥50% stenosis associated with perfusion defect
 in static CTP images.

 At the index visit, failure to complete the CTP protocol will constitute an incomplete study
 and such patients will be excluded from the per protocol analysis. All patients completing
 the CTP protocol and providing informed consent will be included in per-protocol analyses.
 All data from all consented patients who do not complete the CTP protocol will be included in
 the intent-to-diagnose analysis.

 Descriptive statistics will be given for all variables including indications for the test,
 demographics, patient history, CTA test information, dynamic and static CTP information.
 Categorical variables will be summarized as counts and percentages. All continuous variables
 will be summarized as means+/- the standard deviation followed by the median, minimum and
 maximum and 25th, 75th percentiles where needed.

 The frequency of the primary safety outcomes [death, myocardial infarction, unstable angina,
 ventricular tachycardia, asystole, severe bradycardia, allergic skin reactions, allergic
 respiratory reactions, hypotension, anaphylaxis and contrast induced nephropathy] will all be
 summarized; 95% binomial confidence intervals for the proportions of these outcomes will be
 calculated. The additional safety outcomes involving CTP complications will be summarized
 with frequencies and 95% confidence intervals for proportions. Radiation dose and efficiency
 measures (duration(s) involving the CTP procedure) will be summarized overall with median and
 25th and 75th percentiles. Also minima and maxima will be provided. The investigators may
 explore possible associations between patient characteristics [demographics (gender, age),
 prior histories or indications] and the binary safety outcomes using contingency table
 methods, t tests or Wilcoxon Rank Sum, as appropriate. Associations between radiation dose
 and patient characteristics may be explored with nonparametric methods. The investigators may
 also explore possible relationships between the CTA stenosis categories (0%, 1-49%, 50-100%)
 and the dynamic and static CTP results. The occurrence of clinical events recorded at 30-day
 chart review will be related to the category of CTP final results (Normal, Probably Normal,
 Equivocal, Probably Abnormal and Abnormal) using methods for contingency tables which
 incorporate the natural ordering of CTP categories.

 The frequency of the studies with the adequate image quality for interpretation of dynamic
 CTP images will be reported as a measure is feasibility. This feasibility index will be also
 reported at the myocardial segment level and the frequency of Left Ventricular (LV) segments
 with adequate dynamic CTP image quality will be reported.

 The inter- and intra-reader reproducibility of dynamic CTP interpretation will be assessed
 using kappa statistics, intra-class correlation coefficients and Bland-Altman plot, as
 appropriate.

 The accuracy of the dynamic CTP for detection of hemodynamically significant coronary artery
 stenosis will be based on the area under the receiver operating characteristic (ROC) curve
 (AUC) with the static CTP results as the comparator. The correlation of the dynamic vs.
 static CTP will be also assessed in the categorical (Chi-squared test) and continuous
 (Spearman's correlation coefficient) scale.
Inclusion Criteria:

 -  Clinical indication for invasive coronary angiography or CT angiography

 -  Documented coronary artery disease defined as presence of one or more of the
 following:

 -  CAD documented by invasive coronary angiography or CT angiography

 -  History of typical stable angina and receiving guideline-driven therapy for coronary
 artery disease for ≥ 1 month prior to consent

 -  History of hospitalization for unstable angina with no active acute coronary syndrome
 within 48 hours prior to scan

 -  Refractory angina defined as marked limitation of ordinary physical activity or
 inability to perform ordinary physical activity without discomfort, with an objective
 evidence of myocardial ischemia and persistence of symptoms despite optimal medical
 therapy, life style modification treatments, and revascularization therapies

 -  Able to understand and willing to sign the Informed Consent Form.

 Exclusion Criteria:

 -  Known allergy to iodinated contrast media

 -  History of contrast-induced nephropathy

 -  History of multiple myeloma or previous organ transplantation

 -  Elevated serum creatinine (> 1.5mg/dl) OR calculated creatinine clearance of < 60
 ml/min (using the Cockcroft-Gault formula)

 -  Atrial fibrillation or uncontrolled tachyarrhythmia, or advanced atrioventricular
 block (second or third degree heart block)

 -  Evidence of severe symptomatic heart failure (NYHA Class III or IV); Known or
 suspected moderate or severe aortic stenosis

 -  Previous coronary artery bypass or other cardiac surgery

 -  Coronary artery intervention (PCI) within the last 6 months

 -  Known or suspected intolerance or contraindication to beta-blockers including:

 -  Known allergy to beta-blockers

 -  History of moderate to severe bronchospastic lung disease (including moderate to
 severe asthma)

 -  Severe pulmonary disease (chronic obstructive pulmonary disease) with the use of
 inhaled bronchodilators over the past year

 -  Presence of any other history or condition that the investigator feels would be
 problematic

 -  History of high radiation exposure defined as ≥2 nuclear or CT studies or ≥ 5.0 reml
 within 18 months prior to the scan

 -  Does the patient have active acute coronary syndrome within 48 hours prior to consent?

 -  Typical, prolonged (>20 minute) rest angina at admission

 -  Angina equivalent symptoms compatible with ischemia plus abnormal cardiac enzymes

 -  Prolonged rest chest pain (>20 minutes) resolved before admission plus prior
 ischemic ECG changes

 -  Rest chest pain < 20 minutes relieved with nitrates in the prior 48 hours plus
 prior ischemic ECG changes.

 -  If yes to any of the above, Calculate Thrombolysis in Myocardial Infarction
 (TIMI) risk score:

 -  If TIMI risk score ≥ 5 OR elevated cardiac enzymes in the 72 hours prior
 patient is excluded.

 -  If TIMI risk score is <5 and cardiac enzymes are normal patient is included.

 -  If all of above are no then patient is included.

 -  Implantable cardioverter-defibrillator (but not pacemakers) within the imaging field
 of view

 -  Contraindications to vasodilator stress agents:

 -  Systolic Blood Pressure (SBP)<90mmHg, -Recent use of dipyridamole and
 dipyridamole containing medications - -Recent use of methylxanthines
 (aminophylline and caffeine) - -Unstable acute Myocardial Infarction (MI) or
 acute coronary syndrome -

 -  Profound sinus bradycardia (<40 bpm)

 -  Body Mass Index greater than 30