Impact of the Contamination Mode on the Clinical Evolution During Pseudomonas Aeruginosa Ventilator Acquired Pneumonia (PYO GEN)
Impact of the Contamination Mode on the Clinical Evolution During Pseudomonas Aeruginosa Ventilator Acquired Pneumonia (PYO GEN)
Pseudomonas aeruginosa is the main pathogen of nosocomial respiratory infections. Its
 increasing resistance to antibiotics requires the development of new strategies for
 prevention and control, demanding a better understanding of the modes of transmission and
 evolutionary dynamics of this bacteria. In patients under invasive mechanical ventilation,
 the main mode of contamination by Pseudomonas remains debated, with 3 modes of contamination
 (endogenous, crossed transmission between patients, or environmental origin) of varying
 importance, mainly depending on the endemic situation of the place of study.

 The emergence of new genotyping technologies (DiversiLab) can now facilitate studies of
 molecular epidemiology. Thanks to the multidisciplinary collaboration and innovative
 techniques, the investigators wish to study the impact of the mode of contamination on the
 outcome of ICU patients, intubated and ventilated for more than 72 hours.
The presence of environmental reservoirs can cause infections and multidrug-resistant P.
 aeruginosa colonization with P. aeruginosa is itself a prognostic factor, but the impact of
 the route of infection on the evolution of the history and future of the infectious patient
 is not established.

 A second factor that may influence the evolution infectious is the degree of genetic
 heterogeneity of the bacterial population. Multiple exposure pathways could also influence
 the genetic diversity.
Inclusion Criteria:

 -  Patients> 18 years

 -  hospitalized in the intensive care unit

 -  with more than 72 hours of mechanical ventilation

 -  Presenting a positive bacteriological sample P. aeruginosa.

 Exclusion Criteria:

 -  Minors.

 -  Pregnant or lactating women.

 -  Patients under guardianship, under judiciary placement, or hospitalized without their
 consent.

 -  Patients not affiliated to a social security scheme.

 -  Long-term corticosteroid therapy (> 2mg/kg or> 1 month before the onset of established
 infection suspected)

 -  Ongoing chemotherapy, AIDS, transplant patient under immunosuppressive drugs.

 -  Bedridden patient or therapeutic decision at ICU arrival