Application of GeneXpert on Bronchoscopic Samples in the Clinical Management of Patients Suspicious of TB
Application of GeneXpert on Bronchoscopic Samples in the Clinical Management of Patients Suspicious of TB
This is a case control observational study using Bronchial-alveolar lavage (BAL) as specimen
 for GeneXpert, a real time polymerase chain reaction (PCR) test for detection of tuberculosis
 (TB). Patients suspicious of TB, who require bronchoscopy as part of the investigation
 procedures, will be recruited for this study over a period of 18 months. Clinical likelihood
 of TB will be systematically scored according to various clinical parameters. This will
 determine the pre-test probability. Clinician will decide if anti-TB treatment is to be
 started and patients will be followed up for a minimal of 18 months. Sensitivity,
 specificity, positive, negative predictive values and the respective likelihood ratio will be
 calculated accordingly.
Inclusion Criteria:

 1. Patients > 18 years old suspected of TB clinically.

 2. Patients suspected of TB but both smear microscopy and TB PCR of sputum are negative
 or sputum cannot be produced.

 3. BAL samples obtained following standard investigation procedure

 4. CXR or CT imaging available.

 Exclusion Criteria:

 1. Bronchoscopy is not planned as a necessary procedure for investigation.

 2. Diagnosis of active TB has been made by other means.