Diagnostic Accuracy of Polymerase Chain Reaction for Mycobacterium Tuberculosis Using EBUS-TBNA Samples
Diagnostic Accuracy of Polymerase Chain Reaction for Mycobacterium Tuberculosis Using EBUS-TBNA Samples
The purpose of this study is to compare the diagnostic efficacy of nested and realtime
 polymerase chain reaction for Mycobacterium tuberculosis using EBUS-TBNA samples in patients
 with isolated intrathoracic lymphadenopathy.
Although endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) has
 been widely used to perform mediastinal lymph node sampling, little information is available
 on polymerase chain reaction for Mycobacterium tuberculosis (TB-PCR) using EBUS-TBNA samples
 in patients with isolated intrathoracic lymphadenopathy. In addition, two methods of TB-PCR
 (nested PCR and realtime PCR) are available for the detection of Mycobacterium tuberculosis
 and which method is superior for detection of Mycobacterium tuberculosis has not been
 established in patients with isolated intrathoracic lymphadenopathy.

 Thus, this study was design to compare the diagnostic efficacy of nested and realtime TB-PCR
 using EBUS-TBNA samples in patients with isolated intrathoracic lymphadenopathy in South
 Korea, where the incidence of tuberculosis is intermediate (97/100,000 per year).
Inclusion Criteria:

 -  Patients with isolated intrathoracic lymphadenopathy

 Exclusion Criteria:

 -  Suspicion of primary lung cancer on CT or PET scan

 -  Suspicion of pulmonary tuberculosis on CT or PET scan

 -  Suspicion of sarcoidosis of lung parenchyma on CT or PET scan