Chromatic Pupillometry to Assess the Melanopsin-Light Pathway in Progressive Supranuclear Palsy
Chromatic Pupillometry to Assess the Melanopsin-Light Pathway in Progressive Supranuclear Palsy
The specific aim of this study is to investigate rod, cone and melanopsin driven pupillary
 light response in individuals with progressive supranuclear palsy (PSP), age-matched healthy
 controls and individuals with other neurodegenerative diseases using chromatic pupillometry,
 with special interest in assessing melanopsin-driven post-illumination pupil response (PIPR)
 as an identifier for PSP.

 The study addresses the following hypotheses:

 1. Chromatic pupil responses, including rod/cone-driven rapid phase constriction and
 melanopsin-driven PIPR, are reduced in subjects with PSP compared to age-matched normal
 healthy control subjects,

 2. Pupil parameters of the melanopsin-driven PIPR are abnormal in PSP subjects without
 supranuclear palsy, which is indicative of a subclinical physiological deficit of the
 OPN in the early stages of PSP.

 If these hypotheses are upheld, chromatic pupillometry to measure the PIPR promises to be a
 reliable in vivo, non-invasive, convenient and inexpensive technique to detect asymptomatic
 pupillomotor impairment in advance of diagnostic oculomotor signs and deterioration of
 cognitive function.
In 1963, Richardson, Steele and Olszewski published a landmark clinical report on 8 cases of
 supranuclear ophthalmoplegia, pseudobulbar palsy, nuchal dystonia and dementia and
 established the syndrome of heterogeneous system degeneration as a clinicopathological entity
 now known as PSP. The disease has a characteristic onset in the sixth decade (range 45 to 75
 years) with some combination of impaired balance, abrupt falls, visual disturbances, slurred
 speech, dysphasia and vague changes in personality.

 Slowing of voluntary vertical saccades, either down, up or both are a diagnostic marker of
 PSP and later impairment of voluntary horizontal saccades are characteristic in more than
 half of the cases. However, a proportion of PSP patients do not demonstrate these eye signs
 for a year or more after the onset of the disease.

 This pilot study will use chromatic pupillometry to determine whether such a novel
 methodology may be used as an objective in vivo identifier of PSP. The rationale for the
 study is based in part on:

 1. Clinicopathological correlation between the key clinical signs of a supranuclear gaze
 palsy with pathological verification that the degenerative process affects the pretectum
 and rostral midbrain,

 2. The melanopsin-signaling pathway from ipRGCs (intrinsically photosensitive retinal
 ganglion cells) in the eye projects to the OPN (olivary pretectal nucleus) in the
 midbrain,

 3. Chromatic pupillometry is a non-invasive technique suitable for elderly subjects with or
 without dementia.
Inclusion Criteria:

 1. Individuals that meet the clinical criteria for PSP. Core features include:

 -  Recurrent falls and unsteady gait

 -  Axial and nuchal rigidity

 -  Pseudobulbar palsy

 -  Bilateral lid retraction

 -  Supranuclear vertical gaze palsy

 -  Atrophy of the midbrain tegmentum (the hummingbird sign on brain MRI,

 2. Individuals that fit the criteria for the second PSP phenotype (which resembles PD)
 that has asymmetric findings, tremors and poor responses to treatment with Levodopa,

 3. Individuals that meet the clinical criteria for PD with:

 -  Progressive bradykinesia

 -  Postural instability and frequent falls

 -  Festinating gait with loss of associated movements

 -  Cogwheel rigidity and mask-like face

 -  Rest tremor,

 4. Individuals who carry a diagnosis of Alzheimer' disease who present with progressive
 impairment of memory and cognitive domains such as language and visuospatial
 perception.

 Diagnoses will be confirmed by the review of health/medical records of patients recruited
 from the Frontotemporal Disorders Unit clinic. In the case of participants recruited from
 research studies, diagnoses will be confirmed by the review of the research diagnoses
 indicated on the individuals' research records.

 Exclusion Criteria:

 1. Individuals who are frail or in questionable health,

 2. Individuals with cataracts or with posterior pole ocular pathology such as age-related
 macular degeneration and optic neuropathies, including open angle high intraocular
 pressure glaucoma,

 3. Individuals with photophobia (i.e., painful light sensitivity) when exposed to bright
 light, including those with ophthalmological conditions such as keratitis (herpes
 simplex), uveitis or Achromatopsia,

 4. Individuals with advanced dementia with inability to sit erect, hold the eyes open,
 incontinence,

 5. Individuals with epilepsy,

 6. Individuals diagnosed with major depression or other severe psychiatric disorders