Trial of Exercise to Prevent HypeRtension in Young Adults
Trial of Exercise to Prevent HypeRtension in Young Adults
TEPHRA investigates the effect of exercise on blood pressure and other cardiovascular factors
 in young adults with different birth histories. TEPHRA will recruit 200 participants from
 18-35 years old with elevated blood pressure (100 participants pre-term born and 100
 full-term born). Half of the participants from each birth group will be randomised into a 16
 week supervised aerobic exercise intervention trial and the other half will be controls.

 Participants will complete 3 main study visits:

 -  Visit 1: Baseline visit conducted at beginning of study

 -  Visit 2: 16 weeks into study (upon completion of structured exercise intervention for
 the exercise group)

 -  Visit 3: End of study (52 weeks) Each visit will repeat the same set of cardiovascular
 measures including CPET, echo, blood pressure, vascular stiffness, and other measures.

 50 participants from each group will complete an MRI sub-study of the heart, brain, and
 liver.
DESIGN TEPHRA is a randomized control trial investigating the cardiovascular effects (blood
 pressure, structure, and function) of aerobic exercise intervention in young adults with
 elevated blood pressure. The trial will compare outcomes in premature born participants vs
 full-term born participants.

 SCIENTIFIC JUSTIFICATION 1 in 4 adults develop high blood pressure, a risk factor for heart
 disease and stroke. Although high blood pressure is becoming more common in younger adults,
 recent literature review shows that most research on exercise and blood pressure has focused
 on older populations. The effect of exercise on the blood pressure of preterm born young
 adults has not been well studied or reported. Most babies are born full term (between 37-42
 weeks gestation) but 1 in 10 babies are born early (preterm). Evidence suggests that preterm
 birth may be associated with higher blood pressures and differences in the size, shape and
 function of the heart and blood vessels. These differences may alter how a person's blood
 pressure and cardiovascular system respond to exercise. These factors contributed to the
 investigators pursuing this research study.

 The trial is a vehicle to learn more about hypertension in young adults and how exercise
 helps reduce blood pressure. It will investigate the structure and function of the
 cardiovascular system and how it responds to exercise. It will specifically seek to improve
 understanding of how birth histories, such as being born premature may influence blood
 pressure and response to exercise training.

 This trial seeks to illuminate clinically relevant information for one of the most prevalent
 diseases in the UK (hypertension).

 BACKGROUND AND AIMS

 1 in 10 babies are born preterm and studies have identified preterm birth to be associated
 with increased risk of high blood pressure and reduced exercise capacity. This trial aims to
 advance the understanding of how aerobic exercise affects blood pressure and cardiovascular
 structure and function in adults aged 18-35 with different birth backgrounds.

 PARTICIPANTS:

 200 participants (100 preterm born & 100 full term born) aged 18-35 with elevated blood
 pressure.

 RECRUITMENT:

 1. Open advertising

 2. Hypertension Services at the John Radcliffe Hospital

 3. Previous study participants

 4. Local GP surgeries

 RATIONALE AND FUNDING The study rationale and design has been peer-reviewed to secure
 external funding from the Wellcome Trust and has been substantially informed by significant
 interaction with the public and patient involvement group.

 ACRONYMS AE Adverse Event CI Chief Investigator CRF Case Report Form CTRG Clinical Trials &
 Research Governance, University of Oxford GCP Good Clinical Practice GP General Practitioner
 ICF Informed Consent Form NHS National Health Service PI Principal Investigator PIL
 Participant/Patient Information Leaflet R&D NHS Trust R&D Department RCT Randomised Control
 Trial REC Research Ethics Committee SOP Standard Operating Procedure

 RECRUITMENT

 Participants will be recruited via four approaches:

 1. Outpatient Hypertension Clinic, John Radcliffe Hospital, Oxford.

 2. Open advertising targeting Oxford residents and students

 3. Contacting those who gave consent to be contacted for research from previous ethically
 approved studies

 4. Local GP surgeries

 Each approach has been considered from the perspective of the participant and discussed with
 our public and patient involvement group. Collaboration from the clinical team running the
 hypertension service will mirror the process used in the YACHT2 study (NHS REC Form
 Reference:

 14/SC/0275 IRAS Version 3.5 Date: 06/05/2014

 i)Participant Consent The informed consent process is intended to facilitate participants'
 understanding of the study. Full study information will be provided to participants in
 advance of seeking consent, allowing time to interpret the information and ask related
 questions. Trained study investigators will facilitate the informed consent process.
 Participants lacking capacity to provide informed consent will be excluded.

 ii)Participant Safety Prior to enrollment in the study participants will be given opportunity
 to fully consider the study procedures. The study team places emphasis on participants
 understanding that study procedures involve performing a maximal exercise tests and wearing
 monitoring devices (blood pressure, and physical activity) in their home environments as part
 of the study. The team will also emphasize that participants will be randomly placed into
 either the exercise or control group and the exercise group in particular will require a
 substantial commitment of time and physical effort to complete the 16 week intervention
 goals. The intervention support team will provide individual guidance and support to the
 exercise group to support participants in achieving their exercise goals in the safest manner
 possible. The exercise intervention component is designed at an intensity level (60-80% of
 max heart rate) and frequency (3 times per week) that is consistent with standard guidelines
 for safely building aerobic fitness.

 Investigators will emphasize that the MRI sub-study involves undergoing magnetic resonance
 imaging with strong magnetic fields and requires additional screening for contraindications.
 All participants will be fully screened to ensure there are no contraindications to safely
 participating in the study. The study procedures are safe and commonly used in both research
 and clinical practice. Participation in the study does not pose significant risk or burden to
 the participants.

 CONFIDENTIALITY All information and documents collected will be stored securely and kept in
 strict confidence in compliance with Caldicott Principles and the Data Protection Act.

 v)Study samples, investigation results and incidental findings. The use of the participants'
 study samples and investigations are clearly defined in the participant information leaflet
 and will be explained during the informed consent process. The investigations used in the
 study are purely for research purposes but standard operating procedures are in place in the
 unlikely event of incidental findings that are significant to the participants' well-being.
 It is possible that some samples will be used for genetic research. The genetic tests would
 involve looking at common variations in genes that affect how blood vessels work. We do not
 propose to test for inherited genetic diseases. There is no evidence to suggest that the
 results of these genetic studies are likely to have significant implications for the
 participants.

 C. Completion of the Study The participant information leaflets and informed consent process
 clearly explain the intended outcomes of the study and how participant information will be
 stored. At the end of the study, the study team will coordinate dissemination of data from
 this study via journal abstracts, articles, and oral/written presentations. Any scientific
 publications arising from the study will be available on request to all participants as well
 as a summary of the main findings.

 CONSIDERATION OF NULL HYPOTHESES A null hypotheses that "sustained aerobic exercise does not
 reduce blood pressure in preterm born young adults" was not selected. Rather, studies in
 older cohorts (birth backgrounds not assessed) strongly suggests that regular aerobic
 exercise has a blood pressure reducing effect. Previous research has identified differences
 in the structure and function of the heart and blood vessels of preterm born young adults vs
 full term born. Accordingly, the hypothesis that "regular aerobic exercise will elicit
 different blood pressure lowering effects in premature born young adults with elevated blood
 pressure vs full-term born young adults with elevated blood pressure" will be tested in this
 study.

 SAMPLE SIZE SELECTION Recent systematic review and meta-analysis of randomised control trials
 identified an average decrease of 5.4mmHg in systolic blood pressure (SBP) post-intervention
 via supervised exercise in young adults with prehypertension (mean systolic blood pressure at
 baseline 126 mmHg). The post intervention standard deviation of change was 8.7 mmHg. The
 investigators' available cross-sectional pilot data suggests that change will be higher in
 the preterm born participants and participants exposed to maternal hypertension during
 pregnancy. To calculate the sample size, the investigators used an estimated change of 5 mmHg
 following 4 months of intervention. The investigators used a SD of 11.3 from the pooled SD
 for ambulatory systolic blood pressure from cross-sectional pilot data. To observe a
 treatment effect on systolic blood pressure of 5mmHg, powered to 80% (p=0.05) requires a
 total sample size of 164 participants. The SD deviation for the pooled 24 hour awake
 diastolic blood pressure is 8.3 mmHg. To observe a treatment effect on diastolic blood
 pressure of 5mmHg, powered to 80% (p=0.05) requires a total sample size of 114 participants.
 The power calculations for systolic blood pressure are used to determine the final sample
 size, with adjustment to 200 participants to allow for 18% attrition. To ensure primary
 objectives for the study are answered the study team may recruit additional participants to
 replace those who drop out of the study.

 WHY THE DESIGN AND METHODOLOGY WAS SELECTED The TEPHRA design and methodology was selected to
 build on two closely related ongoing studies, EXPRESSO and YACHT2 (summarised below).
 EXPRESSO and YACHT2 collect data that provides significant insight into better characterizing
 the activity norms and cardiovascular systems of young adults with various birth backgrounds.
 TEPHRA advances these research themes by employing an intervention arm (aerobic exercise) to
 investigate the effects of exercise on blood pressure and the structure and function of the
 cardiovascular system.

 JUSTIFICATION FOR INCLUDING A CONTROL ARM Previous studies have identified anatomical and
 functional differences in the cardiovascular systems of preterm born young adults compared to
 full-term born controls. The investigators seek to understand the clinical significance of
 these differences, the response to treatment (in this case exercise) can only be properly
 assessed by comparing the preterm born cohort against controls.

 MINIMISATION OF RESEARCHER EFFECTS AND BIAS

 Due to the nature of the intervention (supervised exercise sessions vs "normal" activity
 levels), TEPHRA is an unblinded trial. However, numerous measures will be taken to minimize
 investigator effects including:

 -  All MRI sub-study images will be assessed by investigators who are blinded to
 participant details

 -- All other images (ie. Ultrasound of heart and small blood vessel imaging) will be
 assessed by investigators who are blinded to participant details

 -  Analysis of all blood samples will be completed by laboratories or investigators who are
 blinded to participant details

 -  Intervention support protocols are standardised for all participants

 -  The intervention team field lead (conducts gym visits etc.) is blinded to participant
 details and does not collect primary study measures

 STUDY PROCEDURES AND DATA COLLECTION

 SCREENING VISITS Screening Visit Procedures (Visit 0, total time 1 hour) Height, weight, body
 composition, and blood pressure will be collected. The investigators will also ask candidates
 to provide information about their medical history and physical activity, and birth history.

 -  Electrocardiogram (ECG) (20 Minutes) An ECG, will be accomplished to check basic heart
 function by placing 12 sticky pads on the chest to record the electrical signals the
 heart makes when it beats. If desired, the investigators will arrange for a candidate's
 preference of a male or female investigator to set up the ECG. The ECG will take about
 20 minutes and will be reviewed by a trained physician. Provided the candidate is happy
 to continue and there are no exclusionary criteria met, an investigator would then fit
 the candidate with a 24 hour blood pressure monitor.

 -  Fitting of 24 hour monitoring equipment (5 minutes) If the screening indicates that the
 candidate may be a good fit for the trial, the study team will also ask the candidate to
 wear a blood pressure monitor for 24 hours. It consists of a blood pressure cuff worn on
 the upper arm that easily fits under clothing. The cuff is attached by a lead to a small
 box which is slightly larger than a deck of cards. This can be placed in a pocket or
 attached to a belt provided by the research team. The cuff will inflate and deflates
 every 30 minutes during the day and every 60 minutes at night to measure blood pressure.
 It is unlikely that it will disturb sleep and participants are asked to power off the
 monitor while driving (the research team will show them how to turn the device on and
 off). A postage paid, addressed envelope will be provided to return the monitor. If all
 the screening measures indicate that the candidate is a good, safe match for the trail
 and would like to proceed, the investigators will arrange a convenient time for them to
 meet for their main trial visits at the Cardiovascular Clinical Research Facility at the
 John Radcliffe Hospital in Oxford.

 MAIN STUDY VISITS COMPLETION OF STUDY VISITS 1-3 (3 hours per visit)

 There are three main visits planned for each participant:

 -  Visit 1: conducted at the beginning of trial (baseline)

 -  Visit 2: conducted ~16 weeks into trial (this visit coincides with completion of the
 structured exercise intervention)

 -  Visit 3: conducted after 52 weeks (at the end of the trial)

 Note - If Visit 1 is within 30 days of the screening and the participant has had no change in
 medical or physical activity status, some basic physical measures (ie. 24 hour blood pressure
 and body composition) will not be re-assessed. Apart from this noted exception, the same
 assessments will be repeated at trial visits 1-3. Participants will be asked to have only
 water for 4 hours before each visit

 -  Questionnaire (30 minutes) This will collect more detailed information about physical
 activities and also lifestyle information such as daily routines and use of alcohol and
 tobacco.

 -  Physical Measures (15 minutes) (as noted, if the baseline visit is within 30 days of
 screening, some measures will not be repeated). THe investigators will measure blood
 pressure and ask participants to walk on the flat for 10 meters while wearing a
 belt-mounted sensor. The participant will be asked to give a urine sample to test for
 the presence of protein as an indicator of cardiovascular and blood vessel function
 related to blood pressure.

 -  Cardiovascular Measures (45 minutes)

 -  Small vessel imaging The investigators will use a special camera to take a picture
 of the blood vessels in the participants' retina. This is a simple, non-invasive
 procedure which uses a special camera that looks through the pupil to observe the
 blood vessels in the back of the eye. This is painless and does not require any
 physical contact with the eye. The investigators may also use a special microscope
 to take video images of the small blood vessels on the back of a finger. This is
 also a painless, non-invasive procedure.

 -  Vascular stiffness measurement The investigators will measure stiffness of the
 blood vessels by placing an inflatable cuff on one arm and also above one knee.
 This is also a painless, non-invasive procedure.

 -  Echocardiogram The investigators will perform an ultrasound (Echocardiogram or
 "ECHO") of the heart. Participants will be asked to disrobe down to the waist and
 put on a hospital gown that is untied at the front to allow the study investigators
 access to place the ultrasound probe on the chest. Participants will lie on a
 padded treatment table on their left side. The probe is placed on chest and
 lubricating jelly is used so the probe makes good contact with the skin. Ultrasound
 waves then create images of the heart on the scanner monitor. The research team are
 trained to perform echocardiograms, and will ensure that privacy is maintained
 throughout the visit. Participants can request that a same gender echocardiographer
 perform the echocardiogram.

 -  Cardiopulmonary Exercise Testing (50 minutes) During the exercise testing, participants
 will be asked to donate a total of up to 150mLs (8 to 9 tablespoons) of blood at each
 trial visit. The blood samples will be stored and analysed for natural compounds and
 genes involved in blood vessel function and cardiovascular disease development. To allow
 for collection of blood samples, a cannula will be placed in a vein in the participants'
 hand or arm. The cannula is a very small, flexible plastic tube that is inserted using a
 needle and allows for collection of blood without the need for multiple needle pricks.
 It is also possible to have conventional blood sampling, involving a number of needle
 pricks, if preferred by the participant.

 Exercise testing will allow assessment of heart and lung function during exercise and is the
 same test used on athletes to see how well they respond to exercise. The exercise test will
 be performed on a stationary bike. We will ask participants to wear appropriate clothing to
 exercise in such as shorts or loose fitting track trousers and a comfortable top (hospital
 scrubs available if needed). Participants will breathe through an opening in a mask worn over
 their mouth and nose whilst exercising which will allow the measurement of oxygen use and
 fitness. Participants may stop the test at any time for any reason. As they cycle on the
 bike, their body response such as heart rate and blood pressure will be recorded.
 Participants will wear a blood pressure cuff on one arm which will be inflated occasionally
 throughout the cycle test. The investigators will place three small sticky pads on
 participants' back to record and ECG during the test.

 The exercise testing lasts approximately 10-15 minutes and participants will pedal against
 gradually increasing resistance. The test is designed to be increasingly difficult as it
 progresses and will be very physically demanding for the last 3-4 minutes. This is likely to
 make participants feel quite exhausted, breathe heavily and their leg muscles may ache
 (particularly if they are not accustomed to exercise). After the test participants will pedal
 against minimal resistance for a cool down.

 --Blood Sampling Before, immediately following, and 20 minutes after the exercise test the
 cannula in the back of the hand or arm will be used to take a sample of blood. This will
 allow the study team to measure changes in blood associated with exercising.

 DATA MONITORING An independent Data Monitoring Committee will monitor and audit the research
 project and data collected.

 ANALYSIS The analysis will be carried out on the basis of intention-to-treat (ITT). This is,
 after randomisation, participants will be analysed according to their allocated intervention
 group irrespective of what they actually receive.

 Patient demographic characteristics and other baseline information will be summarised by
 treatment group. Numbers (with percentages) for binary and categorical variables and mean
 (standard deviation), or median (interquartile or full range) for continuous variables will
 be presented. Normality of variables will be assessed by visual assessment of the normality
 curves and the Shapiro-Wilk test. The analysis of the primary outcome will be assessed using
 analysis of covariance (ANCOVA) adjusting for baseline values and minimisation factors used
 in the randomisation process. Results will be presented as adjusted mean difference in change
 in ambulatory blood pressure between randomised groups at 4 months with 95% confidence
 intervals (CI) and associated two-sided p value. The analysis of secondary outcomes will also
 be done using analysis of covariance (ANCOVA) to establish a co-variant model to examine the
 effect of cardiac structure, vascular function and lifestyle behaviours on exercise capacity.
 Outcomes measured on more than one occasion will be analysed using a mixed effects model. If
 the model assumptions are not met and evidence of departure from Normality is observed,
 transformations of the data will be employed or non-parametric tests will be carried out.
 Statistical analysis will be carried out using STATA or SPSS statistical software.
Inclusion Criteria:

 -  Participant is willing and able to give informed consent for participation in the
 study.

 -  Male or Female, from 18 to 35 years old.

 -  Verified birth history: preterm birth (<37 weeks) or full-term birth (>37 weeks)

 -  Ability to access and use computer/internet

 -  Willing to complete duration of intervention, follow-up and attend study visits at the
 John Radcliffe Hospital (participants may still withdraw at any time without providing
 an explanation or rationale)

 -  24 hour awake ABP greater than 115/75 mmHg

 -  Able (in the investigator's opinion) and willing to comply with all study
 requirements.

 Exclusion Criteria:

 -  Clinic blood pressure greater than 159mmHg systolic and/or 99mmHg diastolic at initial
 screening

 -  Pregnancy

 -  24 hour awake ABP greater than 150mmHg systolic and/or 95mmHg diastolic

 -  Clinic blood pressure greater than 140mmHg systolic and/or 90 mmHg diastolic plus
 evidence of end organ damage secondary to hypertension

 -  Simultaneous participation in another human or clinical randomized trial (if there is
 any possibility of compromising health, safety, or well-being, or any possible
 compromise of study data)

 -  Unable to walk briskly on the flat for 15 minutes

 -  Those currently maintaining levels of cardiovascular fitness and activity at or above
 the levels required for the intervention arm

 -  Unable to attend the regular supervised exercise sessions

 -  Use of beta-blockers such as atenolol or equivalent

 -  BMI >35 kg/m2

 -  Major contra-indications to exercise participation

 -  Evidence of cardiomyopathy

 -  Evidence of inherited cardiac conduction abnormalities

 -  Evidence of congenital heart disease or significant chronic disease relevant to
 cardiovascular status

 The following are exclusion criteria for the MRI sub-study only:

 -  a permanent pacemaker • shrapnel injuries

 -  metal clips in blood vessels of the brain • other metal or electronic implants
 affected by the magnetic field

 -  Unsuitable for MRI based on responses on the MRI safety screening form

 -  an injury to the eye involving fragments of metal