Effects of In-utero Cord Blood Collection on Post-operative Hemoglobin Levels
Effects of In-utero Cord Blood Collection on Post-operative Hemoglobin Levels
Umbilical cord blood (UCB) collection and storage is a rapidly advancing field in hematology
 due to UCB's use for treatment of many hematological diseases, including but not limited to,
 Fanconi anemia, thalassemia, leukaemia and metabolic storage diseases. Primarily, there are
 two ways for UCB harvesting, first in-utero collection and second ex-utero collection.
 Depending on the amount of UCB collected, in-utero harvesting practice prolongs the operative
 time of cesarean delivery and speculatively increases intraoperative blood loss. The aim of
 this study is to determine UCB collection's effect on intraoperative bleeding in women
 undergoing elective cesarean delivery.
Elective cesarean deliveries that occurred in Ankara University Department of Obstetrics and
 Gynecology between March 2014 and February 2015 were enrolled in the study. Deliveries were
 divided into two groups depending whether cord blood was collected or not. All cesarean
 deliveries were performed via Joel-Cohen incisions. Cord blood was harvested in-utero, i.e.
 while leaving the placenta inside the uterus, after delivery of the baby and clamping of the
 cord. After UCB collection, placenta was delivered and hysterotomy site was repaired in one
 layer with continuous locking. Postpartum hemorrhage prophylaxis were performed with one dose
 of 0.2 mg methylergonovine maleate. Postoperatively patients were hydrated with 100cc per
 hour infusion of isotonic liquids and bloods were drawn at eight hours postoperatively for
 control blood counts. Amount of blood loss was estimated by subtracting preoperative
 hemoglobin and hematocrit levels from postoperative hemoglobin and hematocrit. Delta
 hemoglobin and hematocrit levels were compared between two groups. Correlation between amount
 of UCB collected and blood loss was also analysed.

 Patients without a preoperative blood count within 30 days prior to delivery were excluded
 from the study, along with patients with history of bleeding 30 days prior to delivery.
 Emergent cesarean deliveries, postpartum bleeding due to uterine atony, retained placenta,
 cesarean deliveries performed due to placental adhesion abnormalities (placenta previa,
 placenta accreta), patients on anticoagulation regimens (aspirin, low molecular weight
 heparin, heparin), patients with a post-operative haemoglobin level higher than pre-operative
 levels were all excluded from the study. Patients were selected at random from birth register
 by two people who are blind to pre and post-operative blood count results.
Inclusion Criteria:

 -  Elective cesarean deliveries

 -  Women with a blood count results maximum 30 days prior to delivery

 Exclusion Criteria:

 -  Emergent cesarean deliveries

 -  Cesarean deliveries due to placental adhesion abnormalities or placenta previa

 -  Patients on anticoagulation regimens

 -  Postpartum bleeding due to uterine atony, retained placenta

 -  Patients with a history of bleeding 30 days prior to delivery