Identification of Biomarkers in Ischemic Stroke - Clinical Trial
Identification of Biomarkers in Ischemic Stroke - Clinical Trial
The objective of the study is to determine RNA blood biomarker based on 9 genes already
 identified in experimental studies, whose expression would be significantly increased in
 patient with ischemic stroke compared to controls.
This is a monocentric study. It's a case-control study with case being first-ever ischemic
 stroke and controls being healthy (stroke free) subject patients paired for age, sex and
 cardiovascular risk or hemorrhagic stroke paired for age and sex.

 Patients are followed for one year. Blood samples will be taken at inclusion, 12h, 24h, 48h,
 7d, 3m and 1y. Microvesicles samples will be taken at inclusion, 7d and 3M.
Inclusion Criteria:

 -  For ischemic stroke :

 -  Age > 18-year-old

 -  Ischemic stroke diagnosed on clinical presentation and cerebral imaging (CT or MRI
 imaging)

 -  Inclusion inferior to 6 hours from stroke onset

 -  Initial NIHSS score > 0 at the time of clinical examination

 -  Patients with multimodal imaging either through MRI or CT perfusion and supra-aortic
 vessels and intra-cerebral vessels (Willis circle) imaging <0

 -  Procedure of signed consent in situation of emergency : consent of the patient if he
 has the possibility to sign or his representative if he is present

 -  For hemorragic stroke :

 -  Age > 18 year-old

 -  Hemorrhagic stroke diagnosed on clinical presentation and cerebral imaging (CT or MRI
 imaging)

 -  Inclusion inferior to 6 hours from stroke onset

 -  Initial NIHSS score > 0 at the time of clinical examination

 -  Hemorrhagic patients are paired for age and sex with ischemic patients

 -  Procedure of signed consent in situation of emergency : consent of the patient if he
 has the possibility to sign or his representative if he is present

 For healthy controls :

 -  Age > 18 year-old

 -  Stroke-free standardized questionnaire

 -  Initial NIHSS score = 0

 -  Rankin score = 0

 -  High risk cardiovascular subjects

 -  Controls are paired for age, sex and cardiovascular risk measured by a score (European
 Heart Score) with ischemic patients

 Exclusion Criteria:

 -  Not affiliated to social security

 -  Patient under legal protection or deprived of liberty by a judicial or administrative
 decision

 -  Patient whose follow-up will be impossible

 -  Prior stroke

 GROUP FOR ISCHEMIC STROKE :

 - Patients with TIA and a negative cerebral CT or MRI

 GROUP FOR HEMORRAGIC STROKE :

 -  Cerebral hemorrhage related to subarachnoid hemorrhage

 -  Post-traumatic hemorrhage

 -  Hemorrhagic transformation in patients with ischemic stroke

 GROUP FOR HEALTHY CONTROLS :

 - Contraindication MRI