Study to Determine if Serum BNP Levels Are Elevated in Pregnant Women With Pre-Eclampsia
Study to Determine if Serum BNP Levels Are Elevated in Pregnant Women With Pre-Eclampsia
Preeclampsia is a common disease state occurring in the third trimester of pregnancy, with an
 incidence of approximately 5-10% in the US. Hypertension (high blood pressure), a primary
 symptom of pre-eclampsia, may be present in women who were hypertensive prior to becoming
 pregnant.B-type (also known as brain) natriuretic peptide (BNP) is known to be made and
 released from the heart ventricles when the heart is strained. There is also evidence that
 BNP is secreted in the placenta, and may increase in preeclampsia and chronic hypertension in
 pregnancy.The purpose of the study is to determine if a maternal blood sample analyzed for
 the quantity of BNP is helpful in differentiating between pre-eclampsia and chronic high
 blood pressure.
Preeclampsia is a frequent complication of pregnancy. The chief concern is that preeclampsia
 may progress to end-organ damage, with resultant deterioration of both the mother and the
 fetus. As a result, women with significant preeclampsia may have their labor accelerated or
 even require caesarian section. The diagnosis is made based on a constellation of
 non-specific symptoms, including headache, vision changes, proteinuria, and hypertension. The
 diagnosis becomes even more difficult to establish when the patient has chronic hypertension
 prior to her pregnancy.

 In many Emergency Departments across the country, emergency physicians are faced with
 patients that have had either sporadic or non-existent prenatal care. Patients may often not
 know what their blood pressure was before their pregnancy. Records may not exist or be
 available for the emergency physician to make an accurate decision. Patients are often
 transferred to a tertiary care center for obstetrical admission when the diagnosis is
 unclear, as outlying areas are increasingly without obstetrical coverage. The availability of
 a sensitive and specific test for the presence of preeclampsia, or gestational hypertension
 superimposed on chronic hypertension, would allow the emergency physician to avoid costly
 hospital admissions or transfers while provide safe and effective care for the patient.

 B-type or brain type natriuretic peptide (BNP) is a 32-amino acid peptide secreted primarily
 by the cardiac ventricles under conditions of ventricular wall strain. In addition, it has
 been demonstrated that BNP is also secreted in the human amnion during pregnancy. Small
 studies in the international literature suggest that serum BNP is elevated in preeclamptic
 patients and patients with chronic hypertension in pregnancy. A novel fluorescent immunoassay
 technique, the Biosite Triage BNP assay, utilizes murine fluorescent labeled antibodies to
 detect and quantify serum BNP levels. The Triage assay is designed for clinical use, with an
 estimated laboratory turnaround time of fifteen minutes (Biosite Triage BNP product insert).
 The Triage assay has gained acceptance as a highly sensitive and specific tool to assist in
 the rapid diagnosis of congestive heart failure exacerbation; its utility in the preeclamptic
 population has not been assessed.
Inclusion Criteria:

 -  Pregnant women age 18 or older at 34 or more weeks of pregnancy

 -  Control arm:normal blood pressure during pregnancy

 -  Chronic HTN arm: known to have HTN predating this gestation

 -  Pre-eclampsia arm:meet ACOG diagnostic criteria for pre-eclampsia this pregnancy and
 present to labor and delivery for evaluation and/ or treatment

 Exclusion Criteria:

 -  minors, prisoners,unable to provide consent

 -  multiple gestation

 -  currently in labor

 -  Control arm:presence of hypertension, a history of gestational hypertension, current
 therapy with an anti-hypertensive medication, any history of congestive heart failure

 -  Chronic HTN arm:evidence of superimposed preeclampsia at the time of enrollment
 (headache, proteinuria, peripheral edema, or visual changes), any history of
 congestive heart failure

 -  Pre-eclampsia arm:history of congestive heart failure, pretreatment with
 anti-hypertensive medication