Enumeration and Function Analysis of Treg Cells in Peripheral Blood of HCC Patients Before and After Ablation Therapy
Enumeration and Function Analysis of Treg Cells in Peripheral Blood of HCC Patients Before and After Ablation Therapy
The purpose of this study is to determine the enumeration and function changes of regulatory
 t cells in peripheral blood of hepatocellular carcinoma patients before and 1 week, 4 weeks
 after ablation therapy.
Regulatory T cells，which are also called Treg cells，play an important role in suppressing
 anti-tumor immunity.Accumulated evidences indicate that Treg cells are elevated in peripheral
 blood,however,there are also reports that decreased Treg cells are found in hepatocellular
 patients.This study focuses on the changes of Treg cells ratio in peripheral blood of
 hepatocellular carcinoma patients before and 1 week, 4 weeks after ablation ,also its
 functional cytokines,such as TGF-β,IL-10,IFN-γ,and inhibition function when co-cultured with
 CD4+CD25-、CD8+ cells. The investigators speculate that a decreased Treg ratio will be found
 in patients who receive ablation therapy,and its function cytokines,moreover,CD4+CD25-、CD8+
 cells' proliferation and function could be inhibited when co-cultured with Treg cells.
Inclusion Criteria:

 -  hepatocellular patients diagnosed through biopsy;or either dynamic imagine with a
 diagnosis of hepatocellular carcinoma and alphafetoprotein>400μg/L；or two or more
 dynamic imagine with a diagnosis of hepatocellular carcinoma

 -  Child-Pugh A or B

 -  well preserved renal and hematopoietic Function

 -  receive ablation therapy through percutaneous radiofrequency ablation or microwave
 ablation or ethanol injection ablation or any kind of combination of them.

 -  achieve complete ablation accessed by contrast-enhanced CT

 Exclusion Criteria:

 -  incomplete ablation

 -  remote metastasis

 -  Child-Pugh C

 -  general infection

 -  autoimmune diseases

 -  suffer from other tumors concurrently or in last five years

 -  patients with immune deficit or infected by HIV

 -  receiving glucocorticoid or other medicine inhibiting immune system