A Trial to Assess Haploidentical T-depleted Stem Cell Transplantation in Patients With SCD
A Trial to Assess Haploidentical T-depleted Stem Cell Transplantation in Patients With SCD
HSCT is currently the only curative option for SCD but less than 20% of SCD patients have a
 MD donor available. So far, all curative approaches beyond a MSD HSCT at young age are
 non-satisfactory. With the lack of a suitable donor for the vast majority of patients, the
 major question of this trial is, if a haploidentical αß/CD19+ T-cell depleted HSCT can be a
 valid alternative to a MSD HSCT. The main challenge in non-malignant diseases is to offer a
 safe and GvHD-free HSCT without rejection.
Can an α/ß depleted T-Haplo-HSCT with regard to disease free survival, adverse events and
 safety be considered equivalent to a matched sibling donor transplantation (MSD), in order to
 offer cure for the majority of patients with sickle cell disease.

 The main questions of this trial are:

 -  Safety of a α/ß T-depleted haploidentical HSCT

 -  Incidence of acute and chronic GvHD

 -  Rate of rejection

 -  Immune reconstitution

 -  Fertility It is expected that the use of TCRαβ+ and CD19+ depleted haploidentical cell
 grafts in combination with the less aggressive and well tolerated conditioning regimen
 needed for patient preparation will be associated with a low risk of grade II-IV aGVHD
 and no extensive cGvHD, no graft failure and increase speed, spectrum and functionality
 of immune system reconstitution. This is supposed to reduce the incidence of severe
 infections leading to lower rates of transplantation related mortality (TRM).
Inclusion Criteria:

 -  Age 1yr to 35yrs

 -  Homozygous hemoglobin S disease or heterozygous hemoglobin SC or S 0/+

 -  Study specific consent given

 -  Preexisting severe or moderate SCD related complications:

 -  Clinically significant neurological event (stroke) or deficit

 -  Silent crisis, neurocognitive deficit

 -  Pathological angio-MRI with TOF Sequence

 -  TCD velocity >200 cm/s at 2 occasions >1 month apart

 -  More than 5 vaso-occlusive crises (VOC) in the past 1 year or more than 20 VOC in
 a lifetime

 -  Two or more episodes of acute chest syndrome (ACS) in a lifetime or one episode
 of ACS in the past 24 months

 -  Chronic transfusion requirement or more than 8 transfusions or one exchange
 transfusion in a lifetime

 -  Transfusion-refractory allo-immunization

 -  More than five SCD-related hospitalizations in a lifetime

 -  Beginning pulmonary hypertension

 -  Osteonecrosis at more than 2 sites

 -  Beginning SCD Nephropathy

 -  Recurrent priapism (>2)

 Exclusion Criteria:

 -  Karnofsky or Lansky Performance Score < 70%

 -  Patients with donor-specific antibodies (DSA) against the potential stem cell donor by
 either

 -  Cell-based crossmatched assays (Complement-dependent cytotoxicity; CDC) or

 -  Flow cytometry crossmatch test or

 -  Solid-phase immunoassays (SPI) or

 -  Modified SPI such as C4d and C1q assays Whichever method the participating center
 is experienced in.

 -  Patients with major AB0 incompatibility defined according to EBMT Handbook, Edition
 2019 Tab 23.1.:

 ABO incompatibility Recipient Donor Major O A O B O AB A AB B AB

 -  Cardiac function:

 -  Ejection fraction at rest <45.0% on echocardiography or

 -  Shortening fraction of ≥ 27.0% by echocardiogram or radionuclide scan (MUGA)

 -  Patients with > grade II hypertension by Common Toxicity Criteria (CTC)

 -  Renal function:

 -  Estimated creatinine clearance (for patients > 12 years) greater than 50.0
 mL/minute

 -  for pediatric patients (> 1 year to 12 years), GFR estimated by the updated
 Schwartz formula ≥ 90.0 mL/min/1.73 m2. If < 90 mL/min/1.73 m2, renal function
 must be measured by 24-hour creatinine clearance or nuclear GFR and must be >
 70.0 mL/min/1.73 m2 or

 -  Creatinine clearance below threshold defined for stem cell transplantation
 according to local clinical standard

 -  Pulmonary function:

 -  DLCO >50% (adjusted for hemoglobin), and FVC and FEV1≥50%; children unable to
 perform for PFTs, O2 saturation <92% on room air.

 -  Liver function:

 -  Total bilirubin > 2x the upper limit of normal (unless elevated bilirubin is
 attributed to Gilbert's Syndrome) and ALT/AST > 2.5x the upper limit of normal.

 -  Chronic active viral hepatitis

 -  Women who are pregnant (positive serum or urine βHCG) or breastfeeding. Note: Women of
 childbearing potential must have a negative serum pregnancy test at study entry.

 -  Adults of reproductive potential not willing to use an effective method of birth
 control during study treatment and for at least 12 months thereafter,

 -  History of uncontrolled autoimmune disease or on active treatment

 -  Patient unable to comply with the treatment protocol

 -  Prior autologous or allogeneic hematopoietic stem cell transplant

 -  Vaccination with a live virus vaccine during the trial

 -  HIV infection

 -  Patients with a history of psychiatric illness or a condition which could interfere
 with their ability to understand the requirements of the study (this includes
 alcoholism/drug addiction)

 -  Patients unwilling or unable to comply with the protocol or unable to give informed
 consent.

 -  Concurrent severe or uncontrolled medical disease (e.g. uncontrolled diabetes,
 congestive heart failure, myocardial infarction within 6 months prior to the study,
 unstable and uncontrolled hypertension, chronic renal disease, or active uncontrolled
 infection) which by assessment of the treating physician could compromise
 participation in the study