Brain P-gp and Inflammation in People With Epilepsy
Brain P-gp and Inflammation in People With Epilepsy
Background:

 -  The brain is protected by a barrier that keeps toxins in the blood from reaching the
 brain. However, this barrier can also keep useful medications from reaching the brain.
 P-glycoprotein (P-gp) is a brain protein that is part of the blood-brain barrier. The
 level of P-gp is higher in people with epilepsy than in people without epilepsy. These
 different levels of P-gp may explain why some people have seizures that do not respond
 well to medications. Researchers want to see if P-gp can affect the response to epilepsy
 medications.

 -  Epilepsy may also be associated with brain inflammation. Researchers also want to look
 at the part of the brain affected by epilepsy to see if inflammation is present.

 Objectives:

 -  To see if P-gp can affect the response to epilepsy medications.

 -  To see if inflammation is present in the part of the brain affected by epilepsy.

 Eligibility:

 -  <TAB>Individuals between 18 and 60 years of age who have temporal lobe epilepsy. We plan
 to study some patients whose seizures are well controlled by drugs, and some whose
 seizures are not controlled.

 -  <TAB>

 -  Healthy volunteers between 18 and 60 years of age.

 Design:

 -  This study requires four or five visits to the NIH Clinical Center over the course of a
 year. The visits will be outpatient visits and will last from 2 to 5 hours.

 -  Participants will be screened with a physical exam and medical history. Blood and urine
 samples will be collected.

 -  All participants will have two positron emission tomography (PET) scans. The scans will
 take place during different visits. Different drugs will be used in each scan. One drug
 will be used to temporarily block the effect of P-gp in the brain. The other drug will
 show areas of inflammation in the brain.

 -  Participants with epilepsy will have a third PET scan. This scan will also look at P-gp
 activity in the brain. However, it will not use the drug that blocks the effect of P-gp.

 -  All participants will also have one magnetic resonance imaging scan. This scan will help
 show brain function.
Objectives:

 1. To study the role of inflammation in focal epilepsy

 2. To characterize the BBB state in patients with focal epilepsy using MRI, and compare the
 results with PET imaging of inflammation

 3. To study test-retest replicability of [11C]PBR28 PET scanning.

 Study population:

 50 participants with drug-resistant focal epilepsy, 25 participants with drug-responsive
 focal epilepsy and 25 healthy volunteers.

 Design:

 Screening of enrolled participants will include a medical history, physical exam, and blood
 and urine laboratory testing. Blood samples will also be used for genetic polymorphism study.
 Healthy volunteers will receive one or two brain positron emission tomography (PET) scans
 with [11C]PBR28. Epilepsy participants will receive one or two PET scans with [11C]PBR28).
 Everyone will receive a brain magnetic resonance imaging (MRI). Some participants will also
 have a second MRI with gadolinium infusion to measure blood-brain barrier permeability.

 Outcome measures:

 The primary outcome measure will be the amount of differential [11C]PBR28 uptake between the
 epileptic focus and the homologous contralateral region. [11C]PBR28 distribution volume (VT)
 will be measured using an arterial input function. We want to quantify the tracer VT in
 regions of the brain distant from the epileptic focus, which may be affected by the disease.

 We hypothesize that focal epilepsy will be associated with brain inflammation and, therefore,
 that [11C]PBR28 uptake in the affected side of the brain will be higher than in the
 contralateral side.

 We will study the polymorphism of the translocator protein (TSPO), because TSPO polymorphism
 has an influence on [11C]PBR28 binding. This polymorphism is due to the non-conservative
 amino-acid substitution at position 147 from alanine to threonine (Ala147Thr) in the fifth
 transmembrane domain of the TSPO protein.
-  INCLUSION CRITERIA:

 For patients

 -  Age 18-60

 -  Able to give written informed consent.

 -  Drug resistant participants will be defined as having clinically documented partial
 seizures with consistent EEG evidence as defined by the 2010 Revised terminology and
 concepts for organization of seizures and epilepsies and refractory to standard
 antiepileptic treatment for at least one year prior to enrolling in this study. This
 criterion will be established by preliminary screening in the NINDS Clinical Epilepsy
 Section outpatient clinic under protocol 01-N-0139, and if necessary, inpatient
 video-EEG monitoring. Seizure focus localization will be determined by standard
 clinical, neurophysiologic, and imaging studies.

 -  Drug-responsive participants will be defined as having clinically documented partial
 seizures with consistent EEG evidence as defined by the 2010 Revised terminology and
 concepts for organization of seizures and epilepsies, seizure-free on standard
 antiepileptic treatment for at least three months prior to enrolling in this study.
 This criterion will be established by preliminary screening in the NINDS Clinical
 Epilepsy Section outpatient clinic under protocol 01-N-0139, and if necessary,
 inpatient video-EEG monitoring. Seizure focus localization will be determined by
 standard clinical, neurophysiologic, and imaging studies.

 -  No prior diagnosis of drug or alcohol abuse or dependence.

 For healthy volunteers

 -  Age 18-60.

 -  Able to give written informed consent.

 -  No prior diagnosis of drug or alcohol abuse or dependence.

 EXCLUSION CRITERIA:

 For patients

 -  Previous research radiation exposure (X-rays, PET scans etc.) that, together with
 study procedures, would exceed the NIH RSC 5 rad per year research limit.

 -  Claustrophobia to a degree that the subject would feel uncomfortable in the MRI
 machine.

 -  History of brain disease other than epilepsy.

 -  Cannot lie on their back for at least two hours.

 -  Serious medical illness, other than epilepsy.

 -  Clinically significant laboratory abnormalities.

 -  Brain abnormality such as a brain tumor, infection, stroke, brain damage from head
 trauma or blood vessel abnormalities, on an MRI scan.

 -  Pregnancy or breast feeding.

 -  Able to get pregnant but does not use birth control.

 -  Risk for MRI scan, such as a pacemaker or other implanted electrical devices, brain
 stimulators, some types of dental implants, aneurysm clips (metal clips on the wall of
 a large artery), metallic prostheses (including metal pins and rods, heart valves, and
 cochlear implants), permanent eyeliner, implanted delivery pump, or shrapnel
 fragments. Welders and metal workers are also at risk for injury because of possible
 small metal fragments in the eye of which they may be unaware.

 -  PBR28 non-binders.

 -  For drug-responsive subjects: occurrence of a seizure within the last three months.

 -  NIH employee or staff member

 For healthy volunteers

 -  Clinically significant laboratory abnormalities.

 -  Unable to have a MRI scan.

 -  History of neurologic illness or injury with the potential to affect study data
 interpretation.

 -  History of seizures, other than in childhood and related to fever.

 -  Previous research radiation exposure (X-rays, PET scans etc.) that, together with
 study procedures, would exceed the NIH RSC 5 rad per year research limit.

 -  Inability to lie flat on camera bed for at least two hours.

 -  PBR28 non-binders

 -  Pregnancy or breast feeding.

 -  Able to get pregnant but does not use birth control.

 -  NIH employee or staff member