Functional Consequences of T-bet Expression in Flu-specific Memory B Cells After Live Attenuated Influenza Vaccine (LAIV)
Functional Consequences of T-bet Expression in Flu-specific Memory B Cells After Live Attenuated Influenza Vaccine (LAIV)
As yet the investigators do not understand if there are biomarkers of immune protection after
 the Flumist or Live Attenuated Flu Vaccine (LAIV). Here the investigators test the hypothesis
 that the T-bet expressing fraction of flu-specific B cells after live attenuated influenza
 vaccination also serves as an early biomarker of long-lived antibody responses after
 vaccination. In this study the investigators will be providing the LAIV to up to 10 healthy
 subjects and assaying their immune response and then providing the intramuscular influenza
 vaccination and testing to see if the immune protection after the LAIV also protects after
 the intramuscular influenza vaccination.
Previously (1) the investigators established that a fluorochrome labeled reagent with the
 influenza antigen hemagglutinin accurately identified flu-specific B cells after inactivated
 influenza vaccination and we established that a subset of these flu-specfiic B cells that
 express the lineage defining master transcriptional regulator, T-bet, correlate with long
 lived antibody responses. As yet the investigators do not understand if there are biomarkers
 of immune protection after the Flumist or Live Attenuated Flu Vaccine (LAIV). Here the
 investigators test the hypothesis that the T-bet expressing fraction of flu-specific B cells
 after live attenuated influenza vaccination also serves as an early biomarker of long-lived
 antibody responses after vaccination. In this study the investigators will be providing the
 LAIV to up to 10 healthy subjects and assaying their immune response and then providing the
 intramuscular influenza vaccination and testing to see if the immune protection after the
 LAIV also protects after the intramuscular influenza vaccination.
Inclusion Criteria:

 -  Missed getting the flu vaccine in the last year

 -  No history of reactive airway disease

 -  No history of allergy to LAIV

 -  No history of allergy to IIV

 -  Self-identify as healthy without an immunocompromising condition defined as cancer,
 autoimmune disease, organ transplant or receipt of steroids.

 Exclusion Criteria:

 -  History of reactive airway disease

 -  History of allergy to LAIV or IIV

 -  Do not self-identify as healthy