Amyloidosis Typing Using Mass Spectrometry-based Proteomics: a New Accurate Tool for Difficult Typing
Amyloidosis Typing Using Mass Spectrometry-based Proteomics: a New Accurate Tool for Difficult Typing
The main objective is to establish that a new technique of amyloidosis typing by Proteomics
 (based on nanoLC-MS/MS mass spectrometry) allows typing in > 90% of observations in patients
 whose Amyloidosis is reported to have failed typing (impossible typing, uncertain or
 inconsistent) with the traditional anatomopathological approach, and this in a cohort of 40
 patients identified consecutively in the Department of pathological anatomy and cytology of
 the University Hospital of Toulouse and included prospectively.
Inclusion Criteria:

 -  Diagnosis of Amyloidosis by Red Congo staining and birefringence in polarized light on
 tissue sampling

 -  Immunolabeling Amyloidosis typing

 1. impossible (no frozen sample available)

 2. or inconclusive (doubtful)

 3. or inconsistent with clinical, biological, genetic and iconographic data

 -  Signature of the informed consent form

 Exclusion Criteria:

 -  Insufficient tissue material to perform the new technique

 -  Person placed under judicial protection

 -  Pregnant and breastfeeding women