Lung Deposition of Beclometasone Dipropionate/Formoterol Fumarate/Glycopyrronium Bromide Administered Via pMDI in Healthy, Asthmatic and COPD Patients
Lung Deposition of Beclometasone Dipropionate/Formoterol Fumarate/Glycopyrronium Bromide Administered Via pMDI in Healthy, Asthmatic and COPD Patients
The purpose of this study is to investigate the lung deposition and distribution pattern of
 Beclometasone dipropionate/Formoterol Fumarate/Glycopyrronium Bromide using a
 Gamma-scintigraphic technique after inhalation of a single dose of 99mTc radiolabelled CHF
 5993 Administered via pMDI in healthy volunteers, asthmatic and COPD patients.
Inclusion Criteria for all subjects:

 1. Subject's written informed consent obtained prior to any study-related procedure

 2. Body mass index (BMI) between 18 and 32 kg/m2

 3. Ability to understand the study procedures and the risks involved and ability to be
 trained to use the devices correctly at screening and pre-dose.

 4. Normal vital signs (Diastolic BP 60-90 mmHg, Systolic BP 90-140 mmHg or 90-150 if >45
 yrs) at screening and pre-dose.

 5. 12-lead digitalised ECG considered as normal: 60 ≤ heart rate ≤ 100 bpm, 120 ms ≤ PR
 ≤210 ms, QRS ≤ 120 ms, QTcF ≤ 450 ms (male) and ≤ 470 ms (female) at screening and
 pre-dose

 6. Males fulfilling one of the following criteria:

 1. Males with non-pregnant Women of childbearing potential (WOCBP) partners: they
 and/or their partner of childbearing potential must be willing to use a highly
 effective birth control method in addition to the male condom from the signature
 of the informed consent and until 90 days after the follow-up visit. Subjects
 must not donate sperm during the study and for 90 days after the follow-up visit
 or

 2. Males with pregnant WOCBP partner: they must be willing to use male contraception
 (condom) from the signature of the informed consent and until 90 days after the
 follow-up visit. Subjects must not donate sperm during the study and for 90 days
 after the follow-up visit or

 3. Non-fertile male subjects (contraception is not required in this case) or

 4. Males with partner not of childbearing potential (contraception is not required
 in this case).

 7. WOCBP fulfilling one of the following criteria:

 1. WOCBP with fertile male partners: they and/or their partner must be willing to
 use a highly effective birth control method with low user dependency from the
 signature of the informed consent and until 30 days after the follow-up visit or

 2. WOCBP with non-fertile male partners (contraception is not required in this
 case).

 8. Female patients of non-childbearing potential defined as physiologically incapable of
 becoming pregnant (i.e. post-menopausal or permanently sterile). Tubal ligation or
 partial surgical interventions are not acceptable. If indicated, as per investigator's
 request, post-menopausal status may be confirmed by follicle-stimulating hormone
 levels (according to local laboratory ranges)

 Additional Inclusion criteria for Healthy /volunteers and Asthmatic patients:

 9. Males and females aged 18-55

 10. Non- or Ex-smokers who smoked < 5 pack years (pack-years = number of cigarette packs
 per day times the number of years) and stopped smoking > 1 year

 Additional Inclusion Criteria only for Healthy volunteers:

 11. Lung function within normal limits: FEV1 ≥ 80% of predicted (according to the Global
 Lung Function Initiative, ERS Task Force Lung Function Reference Vales) and FEV1/FVC
 ratio > 0.70 at screening

 Additional Inclusion Criteria only for Asthmatic patients:

 12. Pre-bronchodilator 60% ≤ FEV1 ≤ 80% of predicted (according to the Global Lung
 Function Initiative, ERS Task Force Lung Function Reference Vales) and FEV1/FVC ratio
 > 0.70 at screening. If this criterion is not met at Screening, the test can be
 repeated once before Day -1.

 13. Positive reversibility test as defined by an increase in FEV1 ≥ 12% and at least 200
 ml compared to the initial value 20-30 minutes after inhalation of 400 μg Salbutamol
 at screening. If this criterion is not met at Screening, the test can be repeated once
 before Day -1.

 Additional Inclusion Criteria only for COPD patients:

 14. Males and females aged 40-75

 15. Current or ex- smokers (stopped smoking > 1 year ) minimum smoking history of 10
 pack-years (packyears = number of cigarette packs per day times the number of years)

 16. 30% ≤ FEV1 < 50% of predicted and FEV1/FVC < 0.70 after inhalation of 400 μg
 Salbutamol. If this criterion is not met at Screening, the test can be repeated once
 before Day -1.

 Exclusion Criteria for all subjects:

 1. Pregnant or lactating women

 2. Documented history of drug abuse (within 12 months before screening) and/or positive
 urine drug test at screening and/or at treatment period

 3. History of hypersensitivity to M3 Antagonists, β2-agonist, corticosteroids or any of
 the excipients contained in any of the formulations used in the trial;

 4. Any clinically relevant abnormal laboratory value at screening suggesting an unknown
 disease and requiring further clinical investigation or which may impact the safety of
 the subject or the evaluation of the result of the study according to the
 investigator's judgment

 5. Clinically relevant and uncontrolled hepatic, gastrointestinal, renal, genitourinary,
 endocrine, metabolic neurologic, or psychiatric disorder that may interfere with
 successful completion of this protocol

 6. Serology at the screening positive to HIV1 or HIV2 and positive results for Hepatitis
 which indicates acute or chronic Hepatitis B (i.e. positive HB surface antigen HBsAg
 positive and/or positive HB core antibody anti-HBc) or Hepatitis C (HCV antibody);

 7. Subjects with history of sustained and non-sustained cardiac arrhythmias (ECG
 demonstrated) and subjects with a family history of sudden cardiac death

 8. Subjects who have cardiovascular condition such as, but not limited to unstable
 ischemic heart disease, NYHA Class III/IV left ventricular failure, acute ischemic
 heart disease in the last year prior to study screening, which may impact the safety
 of the subject or the evaluation of the result of the study according to the
 investigator's judgment

 9. Medical diagnosis of narrow-angle glaucoma, prostatic hypertrophy or bladder neck
 obstruction that in the opinion of the investigator would prevent use of
 anticholinergic agents"

 10. Significant blood loss ≥ 400 ml within 3 months prior screening and between screening
 and Day 1;

 11. Participation in another clinical trial with an investigational drug in the previous 3
 months before the administration of the study drug; a longer and more appropriate time
 could be considered by the principal investigator based on the elimination of the
 half-life and/or long term toxicity of the previous investigational drug;

 12. Radiation exposure, including that from the present study, excluding background
 radiation but including diagnostic x-rays and other medical exposures, exceeding 5 mSv
 in the last 12 months or 10 mSv in the last 5 years. No occupationally exposed worker,
 as defined in the Ionising Radiation Regulations 1999, shall participate in the study.

 13. Unsuitable veins for repeated venipuncture

 14. Any not allowed concomitant medication

 Additional Exclusion criteria for Healthy volunteers and Asthmatic patients:

 15. Current use of any nicotine or nicotine replacement product

 Additional Exclusion criteria for Asthmatic and COPD patients:

 16. Use of systemic steroids in the 4 weeks prior to screening and between screening and
 Day 1 (injectable depot steroids 6 weeks)

 17. Life-threatening/unstable respiratory status including upper or lower respiratory
 tract infection, within the previous 30 days before screening and between screening
 and Day 1

 18. Requirement for continuous oxygen therapy (supplemental oxygen not exceeding 2 l/min,
 at night only and/or during exercise is allowed)

 Additional Exclusion criteria only for asthmatic patients:

 19. Change in dose or type of any medications for asthma within 4 weeks prior to the
 screening visit

 20. Asthma exacerbation within the 4 weeks prior screening and between screening and Day 1

 21. History of COPD or any chronic respiratory disease other than Asthma

 Additional Exclusion criteria only for COPD patients:

 22. Change in dose or type of any medications for COPD within 4 weeks prior to the
 screening visit

 23. COPD exacerbation within the 4 weeks prior screening and between screening and Day 1

 24. History of asthma or any chronic respiratory disease other than COPD