A Pilot Study of Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) in Generalized Myasthenia Gravis
A Pilot Study of Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) in Generalized Myasthenia Gravis
The purpose of this study is to determine whether the drug Leukine (GM-CFS) is safe and
 tolerated by patients with autoimmune myasthenia gravis (MG).
Twelve patients aged 18-80 with symptomatic generalized autoimmune MG that are not being
 treated with medication that suppresses their immune system, other than prednisone, will
 enter the study at UIC over a two year period. The study will involve a screening visit and
 visits at baseline and at days 5, 15, 30, 45, 60, 90, and 120. The study drug, Leukine
 (GM-CFS), is given by injection. Subjects will give themselves one dose of GM-CSF every day
 for 10 days. Study visits will include muscle testing, immunologic studies and
 quality-of-life studies.
Inclusion Criteria:

 -  Must be between 18 and 80 years of age

 -  Established diagnosis of myasthenia based on: the presence of fatigable weakness of
 ocular, oropharyngeal, and/or limb muscles AND the presence of abnormal acetylcholine
 receptor binding antibodies ≥ 0.4 nmol/l.

 -  Patients of childbearing potential must agree to use a medically acceptable form of
 contraception defined by consistent use of oral contraceptive medications or history
 of tubal ligation or men who are in sexual relationship with such women during and for
 at least 8 weeks following completion of the study.

 -  Patient or designee must have the ability to self-inject investigational product

 -  If thymectomized, the procedure must have been performed at least one year prior to
 screening.

 -  Dose of current anticholinesterase drugs must be constant for 2 weeks prior to
 screening.

 -  If taking prednisone, dose must be stable for ≥4 weeks prior to screening.

 Exclusion criteria:

 -  exclusively ocular MG (MGFA Class I)

 -  severe respiratory and/ or swallowing muscle weakness (MGFA Class Vb or V)

 -  presence of thymoma

 -  Must not have received plasm exchange or IVIG within 4 weeks of screening

 -  Must not have received immuno-modulating agents within the 4 weeks of screening,
 including Azathioprine (Imuran), Cyclosporine (Sandimmune, Neoral), Mycophenolate
 mofetil (CellCept), GM-CSF (Filgrastim; Neupogen; pegfilgrastim, sargramostim), or any
 other chronic immunosuppressive agent

 -  History of tuberculosis or evidence of latent tuberculosis (positive PPD skin test or
 a chest X-ray with evidence of tuberculosis)

 -  vital capacity of less than 1.2 liters or on supplemental oxygen therapy.

 -  severe comorbidities including lung disease, stroke, congestive heart failure of any
 severity, myocardial infarction, EKG abnormalities, uncontrolled hypertension -
 (sitting systolic BP <80 or > 160 mm Hg or diastolic BP > 100 mm Hg, unstable angina
 pectoris, hepatic or renal disease, insulin-dependent diabetes mellitus, history of
 cancer (other than in-situ cervical cancer or resected, cutaneous basal cell or
 squamous cell carcinoma), open cutaneous ulcers, known hepatitis B surface antigen
 (HbsAg) or hepatitis C virus (HCV) positive, or any other concurrent medical
 condition, which would make it unsafe for subjects to participate in the trial or
 interfere with the interpretation of the results.

 -  Laboratories values which, at the time of the screening visit or at any time during
 the study that in the opinion of the Investigator would preclude participation in the
 study including: serum creatinine > 2.5 mg/dL, serum potassium < 3.5 mmol/L or > 5.5
 mmol/L, serum aspartate transaminase (AST), alanine transaminase (ALT), or alkaline
 phosphatase (ALP)> 3 times the upper limit of normal, platelet count < 100,000/mm3,
 WBC count < 3,000 cells/mm3, Hemoglobin, hematocrit, or red blood cell count outside
 30% of the upper or lower limits of normal

 -  Receipt of a live vaccine within 3 months of screening

 -  participation in another investigational drug study within 90 days of screening.

 -  known hypersensitivity to GM-CSF or any of its components

 -  Known HIV-positive status or known history of any other immuno-suppressing disease.

 -  Any mycobacterial disease.

 -  Active severe infections within 4 weeks before screening visit, or between the
 screening and baseline visits.

 -  Untreated Lyme disease.

 -  History of TB or TB exposure, chronic hepatitis B or hepatitis C, SLE, history of
 multiple sclerosis, transverse myelitis, optic neuritis or epilepsy.

 -  History of recent alcohol or substance abuse (< 1 year)

 -  Pregnant or lactating females

 -  History of non-compliance with other therapies

 -  abnormal mental status sufficient to exclude informed consent

 -  History of any opportunistic infection - to include but not limited to Pneumocystis
 carinii, aspergillosis, histoplasmosis, or atypical mycobacterium

 -  History of Sickle cell disease.