Chronic Intermittent Cold Exposure on Weight Loss
Chronic Intermittent Cold Exposure on Weight Loss
1. To test whether chronic intermittent cold exposure is more effective in programmed body
 weight loss when compared to conventional programmed weight loss.

 2. To measure the metabolic responses (e.g. in the white and brown adipose tissue, muscle
 and circulating markers) of treatment with chronic intermittent cold exposure and
 conventional treatment

 3. To measure tissue specific changes of weight loss and the treatment with chronic
 intermittent cold exposure in glucose uptake rate in adipose organ (white and brown
 adipose tissue), liver, myocardium, skeletal muscle and brain

 4. To measure tissue specific biochemical indicators before and after treatment with
 chronic intermittent cold exposure from white adipose tissue and skeletal muscle.

 5. To compare changes in body composition and fat distribution after weight loss using
 chronic cold exposure or using conventional method
Inclusion Criteria:

 -  BMI > 30 (inclusive)

 -  Body weight less than 120 kg

 -  Weight stability for 3 months

 -  Plasma triglyceride concentration less than 3.0 mmol/l

 -  Plasma HDL-cholesterol more than 0.9 mmol/l in males and more than 1.0 mmol/l in
 females

 -  Blood pressure less than 140/90 mmHg (inclusive)

 -  Normal glucose tolerance or impaired glucose tolerance but not diabetes (fasting
 glucose < 7.0 mmol/l or 2 h glucose in the oral glucose tolerance test < 11.1 mmol/l)

 Exclusion Criteria:

 -  Smoking

 -  Frequent weight loss attempts

 -  Pregnancy or lactation

 -  Diabetes mellitus

 -  Hypo/hyperthyreosis

 -  Cardiovascular disease, stroke or neurological disorder

 -  Medication (antihypertensive and antidiabetic drugs, lipid lowering drugs,
 antidepressants, corticosteroids)

 -  Claustrophobia

 -  Any other condition that in the opinion of the investigator could create a hazard to
 the subject safety, endanger the study procedures or interfere with the interpretation
 of study results.

 -  Presence of any ferromagnetic objects that would make MRI imaging contraindicated