Use of Faecal Calprotectin to Predict Enteropathy of the Preterm Neonates
Use of Faecal Calprotectin to Predict Enteropathy of the Preterm Neonates
This study aims to evaluate a clinico-biological predictive score, associating the faecal
 calprotectin, for the diagnosis of enterocolitis and enteropathy of the preterm neonates.
Enteropathy and necrotizing enterocolitis (NEC) are digestive emergencies in premature
 neonates which represent a major concern for the vital prognosis. It is therefore important
 to propose a tool for early diagnosis of these intestinal complications in order to avoid
 extended interruptions of enteral feeding at a period of life where growth (and digestive
 maturation) is in the foreground. Currently, the diagnosis of digestive impairment is based
 on classical clinical, biological (CRP, procalcitonin, fecal calprotectin, NFS) and
 radiological (abdomen without preparation) approaches, which induce on one hand a prolonged
 interruption of enteral feeding sometimes useless and even harmful for the intestinal
 maturation and, secondly, the establishment of a parenteral nutrition which is not risk-free.
 The study propose to dose the fecal calprotectin, a non-invasive marker of digestive
 inflammation, in order to validate the threshold decision proposed in a pilot study (350 µg/g
 of feces). Measurement of fecal calprotectin levels will be used as an integrated tool to
 build a powerful screening score combined to the usual parameters used in routine care for
 the diagnosis of and enteropathy and necrotizing enterocolitis (NEC) in premature neonates. A
 cohort of preterm infants born at a gestational age of 33 weeks or less will be included from
 birth. A stool sample will be collected from the diaper once a week, from birth until
 discharge from the hospital, and every day during gastrointestinal events (interruption of
 enteral feeding over 48 hours). The study will evaluate the performance of the rapid assay of
 fecal calprotectin by quantitative immunochromatography compared to the reference method by
 enzyme-linked immunosorbent assay (ELISA).
Inclusion Criteria:

 -  Infants prematurely born at a gestational age of 33 weeks or less (or before the 7th
 month of pregnancy).

 -  Patient whose parents or holders of parental authority signed an informed consent.

 Exclusion Criteria:

 -  Patient with a malformation.

 -  Lack of signature of the consent by parents or holders of parental authority.