A2 AR as a Novel Biomarkers for Physician Decision-making Improvement Evaluation's Patients With Suspected Acute Coronary Syndrome But Negative Troponin.
A2 AR as a Novel Biomarkers for Physician Decision-making Improvement Evaluation's Patients With Suspected Acute Coronary Syndrome But Negative Troponin.
One proposed strategy is the stratification of troponin-negative patients with biomarker
 testing at presentation to facilitate the clinically-appropriate rapid discharge from the
 emergency department of patients who present with low-intermediate risk chest pain, and
 conversely to triage appropriate Non sustained ST elevation acute coronary syndrome
 (NSTE-ACS) patients to Cardiology beds, stress and non-invasive imaging modalities.
 Biomarkers such as high-sensitivity troponin (hs-cTn), heart-type fatty acid-binding protein
 (H-FABP), CRP, brain natriuretic peptide (BNP); and copeptin and ischemia-modified albumin
 are an important advance for diagnostic testing for ACS (4). Regarding novel biomarker
 testing at presentation, the addition of these biomarkers demonstrated increased sensitivity
 at an acceptable QALY threshold, but more evidence is needed (5,6). A reliable method for the
 diagnosis of minimal cardiac ischemia would meet a strong demand for the sensitive diagnosis
 of coronary artery disease in patients with chest pain but unremarkable ECGs and biomarkers.
 Adenosine is an endogenous nucleoside cardioprotective agent. Its cardiovascular effects are
 mediated throught the activation of A2A Receptor (A2 AR) and play a major role in the
 regulation of Coronary flow CF. As altered coronary blood flow occurs in patients with CAD,
 it has been showed that that A2AR expression and functional activity play a role in CAD. In a
 previous studies the team have developped an agonist-like monoclonal antibody to study
 expression level of this receptor and their functional activity. Recently , Gariboldi
 demonstrated that measuring the expression level of A2AR on peripheral blood mononuclear
 cells ( PBMC) represents a good tool to address in situ expression in coronary tissues of CAD
 patients.
In the diagnostic setting of ACS, expression and functionnaly activity of A2AR could be a
 sensitive detection method for ischemia. It would be a powerful tool for risk stratification
 of patients presenting with chest pain but unremarkable ECG and blood tests. the
 investigateors therefore designed a blind multicentrique prospective study to evaluate
 expression and functionnaly activity of A2AR in the management of undifferentiated chest pain
 The objective of the study was to evaluate diagnostic accuracy between this novel biomarkers
 A2AR and invasive and non -invasive evaluation of patients with suspected coronary artery
 disease (CAD).
Inclusion Criteria:

 -  A subject with a symptomatology consistent with acute coronary syndrome for at least
 15 min and less than 6 hours (eg discomfort, tightness or chest pain, pain radiating
 to the left arm or both arms, pain in the jaw, Pain in the back / neck / stomach,
 dyspnea, cold sweats, nausea / vomiting, dizziness)

 -  Man or woman,

 -  over 18 years,

 -  Patient agreeing to participate in the study and having signed informed consent.

 Exclusion Criteria:

 -  Minor Patient

 -  Patient not having signed informed consent (refusal, physical or mental incapacity
 ...) --Patient with an evolutive septic process, neoplasia undergoing treatment,
 dialysed renal insufficiency, history of surgery or coronary angioplasty less than six
 months old.

 Cardiac, renal or hepatic transplantation

 -  elevation of troponin

 -  Cardiac arrest

 -  A subject whose symptomatology clearly eliminates acute coronary syndrome (penetrating
 trauma, traumatic lesion by crushing ...)

 -  Patient who died between the time of inclusion and arrival in cardiology intensive
 care (ICU)

 -  Patient withdrawing consent during study

 -  Vulnerable people unable to fully integrate the objectives, benefits and risks of
 research and to give their informed consent

 -  Pregnant women

 -  Persons deprived of their liberty

 -  Persons admitted to health and social facilities for therapeutic purposes

 -  Protected persons