A Study Comparing Amorphous Calcium Carbonate (ACC) Versus Crystalline Calcium CCS) in Hypoparathyroidism Patients
A Study Comparing Amorphous Calcium Carbonate (ACC) Versus Crystalline Calcium CCS) in Hypoparathyroidism Patients
Primary objective:

 Phase I Proof of concept: treatment with smaller doses of elemental calcium from ACC compared
 to CCS can maintain target serum calcium (corrected for albumin) values (7.0-10.0 mg/dL).

 Phase II To test the hypothesis that treatment with smaller doses of elemental calcium from
 ACC compared to CCS can maintain target serum calcium (corrected for albumin) values
 (7.0-10.0 mg/dL).

 Secondary objectives:

 Phase I

 -  ACC dose selection - to confirm the conversion factor of ACC from CCS

 -  To determine the effect of food on ACC absorption

 Phase II

 -  To test the hypothesis that treatment with smaller doses of elemental calcium from ACC
 compared to CCS will not cause an increase in hypercalciuria in patients with
 hypoparathyroidism

 -  To test the hypothesis that smaller doses of elemental calcium from ACC can reduce the
 side effects related with high calcium consumption.

 Amorphical has a strong basis to believe that the ACC product is better absorbed compared to
 the commercially available CCS products and therefore, can maintain desirable target albumin
 corrected calcium values in serum (CA) with smaller doses of elemental calcium from ACC. As
 results, the burden of taking high doses of calcium supplementation along with the side
 effects of the standard therapy (gastrointestinal discomfort and hypercalciuria) will be
 reduced.

 Testing serum CA and urine calcium values in subjects with hypoparathyroidism may provide a
 straightforward method to test this hypothesis.

 The study is designed to be conducted with extra precaution in order to avoid disturbing the
 fragile balance between CA levels in serum and calcium levels urine. The crossover design of
 phase II of the study allows a more accurate and reliable comparison of results attributable
 to the specific treatment within the same individual. In addition, the subjects will continue
 consuming all their routine medication throughout the trial. The subjects in the control arm
 will consume their routine calcium supplement doses thus, will be treated with a standard of
 care.
Eligible subjects will be treated as follows:

 Phase I Ten (10) subjects previously diagnosed and chronically treated for primary
 hypoparathyroidism will be enrolled. The daily CCS intake will be gradually replaced by
 reduced amount of elemental calcium from ACC. Five (5) subjects will consume the ACC before
 having a meal and the other five (5) subjects will consume the ACC after having a meal. The
 safety and the efficacy of the treatment will be closely monitored throughout this phase.

 The absorption of ACC will be evaluated using weekly serum calcium corrected for albumin (CA)
 value tests. Excretion of calcium in urine will be tested at screening and at the end of
 phase I.

 I-Day -21 (+/-17) Screening: Subjects with a diagnosed primary hypoparathyroidism (see
 section 4.1 for definitions), and who are treated with calcium and vitamin D supplementation
 at least 1 year prior to the beginning of the study and are without major renal or hepatic
 disease, will be invited to the Clinical Research Center. At the clinic, subjects will be
 interviewed, their medical history and their current medication will be documented and they
 will sign an informed consent form (ICF). Subjects will be referred to perform blood tests
 for serum calcium, P, creatinine and albumin levels. Calculation of albumin corrected calcium
 (CA) will be performed. Subjects will be instructed to perform 24 hour urine collection for
 Ca, P and creatinine. Subjects will be asked to fill out a food and medication diary for 3
 consecutive days to evaluate their daily dietary calcium intake. Women of childbearing age
 will undergo a urine pregnancy test. Eligible subjects, complying with all inclusion criteria
 and having none of the exclusion criteria will be enrolled to the study.

 Subjects will be informed by phone or on site whether they are eligible to enter the study.

 I-Day 0: Eligible subjects will arrive at the Clinical Research Center where they will be
 asked about any changes in their medical condition since their last visit. Blood tests will
 be performed to define serum calcium, P and albumin baseline values. Calculation CA at
 baseline will be performed.

 Subjects will receive a pack of ACC tablets, each tablet containing 50, 100 or 200 mg
 elemental calcium (according to the daily total amount of calcium supplementation, 14 day
 supply + 5 spare tablets). The replacement of CCS with ACC will be calculated according to
 the following formula:

 NTDC = ITDC - [0.1×ITDC (mg CCS)] + [0.05×ITDC (mg ACC)]

 *ITDC - Initial total daily calcium intake (mg)

 **NTDC - New total daily calcium intake (mg)

 10% (in mg) out of the initial total daily intake of elemental calcium will be replaced by 5%
 of elemental calcium from ACC (in mg, calculated out of the initial total daily intake). The
 daily intake of vitamin D will remain the same.

 The calculation for the number of tablets per day will be performed specifically for each
 subject (according to the daily dosage of calcium supplementation) by the doctor.

 Subjects will be instructed to take XXX ACC tablets a day, according to their individual
 calculated NTDC:

 1. Five subjects will be instructed to take XXX tablets in the morning after a meal, XXX in
 midday after a meal and XXX in the evening after a meal.

 2. Five subjects will be instructed to take XXX tablets in the morning before having a
 meal, XXX in midday before having a meal and XXX in the evening before having a meal.

 Subjects will be instructed to continue their routine medications consumption during the
 trial.

 I-Day 3 (±1): Subjects will arrive at the Clinical Research Center and their serum calcium, P
 and albumin levels will be tested. Calculation of CA will be performed to exclude
 hypocalcaemia (Ca<7.0 mg/dL). If CA values are within the desired target range (7.0-10.0
 mg/dl), subjects will continue to take the calcium doses that were instructed on I-day 0. If
 CA values are below 7.0 mg/dl or above 10.0 mg/dl, changes to the calcium intake will be
 made, according to the doctor's decision.

 I-Day 7 (±1): Subjects will arrive at the Clinical Research Center and their serum calcium, P
 and albumin levels will be tested. Calculation of CA will be performed:

 1. Conversion factor 0.5:

 If CA = baseline, then 20% (in mg, calculated out of the initial total daily intake) of
 elemental calcium will be replaced by 10% of elemental calcium from ACC (in mg,
 calculated out of the initial total daily intake).

 NTDC = ITDC - [0.2×ITDC (mg CCS)] + [0.1×ITDC (mg ACC)]

 2. Conversion factor 0.75:

 If CA < baseline, then 10% (in mg, calculated out of the initial total daily intake) of
 elemental calcium will be replaced by 7.5% of elemental calcium from ACC (in mg,
 calculated out of the initial total daily intake).

 NTDC = ITDC - [0.1×ITDC (mg CCS)] + [0.075×ITDC (mg ACC)] CA <7.00 mg/dl will enforce
 end of treatment.

 3. Conversion factor 0.25:

 If CA > baseline, then 10% (in mg, calculated out of the initial total daily intake) of
 elemental calcium will be replaced by 2.5% of elemental calcium from ACC (in mg, calculated
 out of the initial total daily intake).

 NTDC = ITDC - [0.1×ITDC (mg CCS)] + [0.025×ITDC (mg ACC)] Subjects will be asked about any
 side effects or AEs that may have occurred and changes in concomitant medications since their
 last visit. Subjects will be asked about symptoms and signs related with a change in serum
 calcium levels (tetany, facial grimacing, paresthesias, muscle aches, arrhythmia,
 depression). Subjects will receive instructions regarding the new doses of ACC and will be
 reminded to take XXX tablets a day (according to their individual calculated NTDC), XXX in
 the morning, XXX in midday and XXX in the evening, before or after a meal (based on their
 initial assignment). Subjects will be reminded to continue their routine medications
 consumption during the trial.

 I-Day 10 (±1): Subjects will arrive at the Clinical Research Center and their serum calcium,
 P and albumin levels will be tested. Calculation of CA will be performed to exclude
 hypocalcaemia (Ca<7.0 mg/dL). If CA levels are within the desired target range (7.0-10.0
 mg/dl), subjects will continue to take the calcium doses that were instructed on I-day 7. If
 CA levels are below 7.0 mg/dl or above 10.0 mg/dl, changes to the calcium intake will be
 made, according to the doctor's decision.

 I-Day 14 (±1): Subjects will arrive at the Clinical Research Center and their serum calcium,
 P and albumin levels will be tested. Calculation of CA will be performed to exclude
 hypocalcaemia (Ca<7.0 mg/dL). If none of the conversion formulas (a-c, I-Day 7) resulted in
 serum calcium values within the desired target range (7.0-10.0 mg/dL), the study will be
 terminated.

 Subjects will be asked about any side effects or AEs that may have occurred and changes in
 concomitant medications since their last visit. Subjects will be asked about symptoms and
 signs related with a change in serum calcium levels (tetany, facial grimacing, paresthesias,
 muscle aches, arrhythmia, depression).

 50% of the initial daily supplementation of CCS will be replaced by ACC based on the
 conversion factor found in I-day 7 (formulas a-c):

 1. Conversion factor 0.5: 50% (in mg, calculated out of the initial total daily intake) of
 elemental calcium will be replaced by 25% of elemental calcium from ACC (in mg,
 calculated out of the initial total daily intake).

 NTDC = ITDC - [0.5×ITDC (mg CCS)] + [0.25×ITDC (mg ACC)]

 2. Conversion factor 0.75: 50% (in mg, calculated out of the initial total daily intake) of
 elemental calcium will be replaced by 37.5% of elemental calcium from ACC (in mg,
 calculated out of the initial total daily intake).

 NTDC = ITDC - [0.5×ITDC (mg CCS)] + [0.375×ITDC (mg ACC)]

 3. Conversion factor 0.25: 50% (in mg, calculated out of the initial total daily intake) of
 elemental calcium will be replaced by 12.5% of elemental calcium from ACC (in mg,
 calculated out of the initial total daily intake).

 NTDC = ITDC - [0.5×ITDC (mg CCS)] + [0.125×ITDC (mg ACC)] Subjects will receive a pack of ACC
 tablets, each tablet containing 50, 100 or 200 mg elemental calcium (according to their
 individual calculated NTDC, 14 day supply + 5 spare tablets). Subjects will receive
 instructions regarding the new doses of ACC and will be reminded to take XXX capsules a day,
 XXX in the morning, XXX in midday and XXX in the evening, before or after a meal (based on
 their initial assignment). Subjects will be reminded to continue their routine medications
 consumption during the trial.

 I-Day 21 (±1): Subjects will arrive at the Clinical Research Center and their serum calcium,
 P and albumin levels will be tested. Calculation of CA will be performed to exclude
 hypocalcaemia (Ca<7.0 mg/dL). Subjects will be asked about any side effects or AEs that may
 have occurred and changes in concomitant medications since their last visit. Subjects will be
 asked about symptoms and signs related with a change in serum calcium levels (tetany, facial
 grimacing, paresthesias, muscle aches, arrhythmia, depression). If CA levels are below 7.0
 mg/dl, or above 10.0 mg/dl, subject will be excluded from the study (based on the doctor's
 decision). If CA levels are within the desired target range (7.0-10.0 mg/dL), a complete
 replacement of the daily supplementation of CCS with ACC will be performed, based on the
 conversion factor found in I-day 7 (formulas a-c):

 1. Conversion factor 0.5: 100% (in mg) of the elemental calcium initial total daily intake
 will be replaced by 50% of elemental calcium from ACC (in mg, calculated out of the
 initial total daily intake).

 NTDC = ITDC - [ITDC (mg CCS)] + [0.5×ITDC (mg ACC)]

 2. Conversion factor 0.75: 100% (in mg) of the elemental calcium initial total daily intake
 will be replaced by 75% of elemental calcium from ACC (in mg, calculated out of the
 initial total daily intake).

 NTDC = ITDC - [ITDC (mg CCS)] + [0. 75×ITDC (mg ACC)]

 3. Conversion factor 0.25: 100% (in mg) of the elemental calcium initial total daily intake
 will be replaced by 25% of elemental calcium from ACC (in mg, calculated out of the
 initial total daily intake).

 NTDC = ITDC - [ITDC (mg CCS)] + [0.25×ITDC (mg ACC)] Subjects will receive instructions
 regarding the new doses of ACC and will be reminded to take XXX tablets a day, XXX in the
 morning, XXX in midday and XXX in the evening, before or after a meal (based on their initial
 assignment). Subjects will be reminded to continue their routine medications consumption
 during the trial.

 Subjects will receive a container to perform 24 hour urine collection prior to their next
 scheduled visit.

 I-Day 27 (±1) by phone: Subjects will be reminded to perform 24 hour urine collection.

 I-Day 28 (±1) - Termination of phase I: Subjects will arrive at the Clinical Research Center
 and their serum calcium, P and albumin levels will be tested. Calculation of CA will be
 performed. Subjects will provide the container of 24 hour urine collection for Ca, P and
 creatinine to test calciuria. Subjects will be asked about any side effects or AEs that may
 have occurred and changes in concomitant medications since their last visit. Subjects will be
 asked about symptoms and signs related with a change in serum calcium levels (tetany, facial
 grimacing, paresthesias, muscle aches, arrhythmia, depression).

 Phase I results will be examined before deciding whether or not to embark on the crossover
 portion of the study planned for Phase II. Phase I data will be summarized, showing for each
 subject, by arm and overall the relationship between CA levels by amount of ACC replacement
 of CCS received.

 Phase II Ten (10) subjects previously diagnosed and chronically treated for primary
 hypoparathyroidism will be enrolled. Subjects participated in phase I will be offered to
 participate in phase II as well. If needed, new subjects will be enrolled.

 The subjects will be randomly assigned to one of the following treatments for 6 weeks:

 1. Standard of care (CCS) - The same elemental calcium dosage that was used routinely prior
 to the study.

 2. ACC - The established dosage of elemental calcium from ACC (based on the conversion
 factor and the fed/fasted conditions found in phase I of the study).

 The two formulations will be administered with the regular daily dosage of vitamin D (1-alfa
 D3).

 At the end of the treatment, each group will receive the alternative formulation for another
 6 weeks.

 The superior absorption of ACC will be evaluated using weekly blood tests to calculate serum
 CA values. Excretion of calcium in urine will be tested at screening and in the end of each
 treatment.

 II-Day -21 (+/-17) Screening: Subjects with a diagnosed primary hypoparathyroidism (see
 section 4.1 for definitions), and who receiving calcium and vitamin D supplementation at
 least 1 year prior to the beginning of the study and are without major renal or hepatic
 disease, will be invited to the Clinical Research Center . At the clinic, subjects will be
 interviewed, their medical history and their current medication will be documented and they
 will sign an informed consent form (ICF). Subjects will be referred to perform blood tests
 for Calcium, P and albumin levels. Calculation of CA will be performed. Subjects will be
 instructed to perform 24 hour urine collection for Ca, P and creatinine levels. Subjects will
 be asked to fill out a food and medication diary for 3 consecutive days to evaluate their
 daily dietary calcium intake. Women of childbearing age will undergo a urine pregnancy test.
 Eligible subjects, complying with all inclusion criteria and having none of the exclusion
 criteria will be enrolled to the study.

 Subjects will be informed by phone or on site whether they are eligible to enter the study.

 II-Day 0: Eligible subjects will arrive at the Clinical Research Center where they will be
 asked about any changes in their medical condition since their last visit. Blood tests will
 be performed to define serum calcium, P and albumin baseline values. CA values will be
 calculated at baseline.

 Subjects will be randomly assigned to one of the following treatments:

 1. Standard-of-care (CCS) - The same elemental calcium dosage that was used routinely prior
 to the study.

 2. ACC - The established dosage of elemental calcium from ACC (based on the conversion
 factor and the fed/fasted conditions found in phase I of the study).

 Subjects that were assigned to the ACC treatment arm, will receive a pack of ACC tablets,
 each tablet containing 200 mg elemental calcium (35 day supply + 5 spare tablets). Subjects
 assigned to the CCS treatment arm will continue to take their routine calcium
 supplementation.

 The exact dosage of calcium supplementation will be determined for each subject according the
 known medical history.

 Subjects will be instructed to continue their routine medications consumption during the
 trial.

 II-Day 3 (±1): Subjects will arrive at the Clinical Research Center and their serum calcium,
 P and albumin levels will be tested. Calculation of CA will be performed to exclude
 hypocalcaemia (Ca<7.0 mg/dL). If CA values are within the desired target range (7.0-10.0
 mg/dl), subjects will continue to take the calcium doses that were instructed on II-day 0. If
 CA levels are below 7.0 mg/dL or above 10.0 mg/dL, changes to calcium intake will be made,
 according to the doctor's decision

 II-Day 7 (±1): Subjects will arrive at the Clinical Research Center and their serum calcium,
 P and albumin levels will be tested. Calculation of CA will be performed. Subjects will be
 asked about any side effects or AEs that may have occurred and changes in concomitant
 medications since their last visit. Subjects will be asked about symptoms and signs related
 with a change in serum calcium levels (tetany, facial grimacing, paresthesias, muscle aches,
 arrhythmia, depression). Adjustments of ACC or CCS intake will be performed if necessary.

 Subjects will be reminded to take the calcium supplementation according to their assignment
 instructions.

 Subjects will be reminded to continue their routine medications consumption during the trial.

 II-Day 10 (±1): Subjects will arrive at the Clinical Research Center and their serum calcium,
 P and albumin levels will be tested. Calculation of CA will be performed to exclude
 hypocalcaemia (Ca<7.0 mg/dL). If CA levels are within the desired target range (7.0-10.0
 mg/dl), subjects will continue to take the calcium doses that were instructed on II-day 7. If
 CA levels are below 7.0 mg/dL, or above 10.0 mg/dL, changes to the calcium intake will be
 made, according to the doctor's decision

 II-Day 14 (±1): Subjects will arrive at the Clinical Research Center and their serum calcium,
 P and albumin levels will be tested. Calculation of CA will be performed. Subjects will be
 asked about any side effects or AEs that may have occurred and changes in concomitant
 medications since their last visit. Subjects will be asked about symptoms and signs related
 with a change in serum calcium levels (tetany, facial grimacing, paresthesias, muscle aches,
 arrhythmia, depression).

 Subjects will be reminded to take the calcium supplementation according to their assignment
 instructions.

 Subjects will be reminded to continue their routine medications consumption during the trial.

 II-Day 21 (±1): Subjects will arrive at the Clinical Research Center and their serum calcium,
 P and albumin will be tested. Calculation of CA will be performed. Subjects will be asked
 about any side effects or AEs that may have occurred and changes in concomitant medications
 since their last visit. Subjects will be asked about symptoms and signs related with a change
 in serum calcium levels (tetany, facial grimacing, paresthesias, muscle aches, arrhythmia,
 depression).

 Subjects will be reminded to take the calcium supplementation according to the instructions.

 Subjects will be reminded to continue their routine medications consumption during the trial.

 Subjects will receive a container to perform 24 hour urine collection prior to their next
 scheduled visit.

 II-Day 34 (±1) by phone: Subjects will be reminded to perform 24 hour urine collection.

 II-Day 35 (±1): Subjects will arrive to the Clinical Research Center and their serum calcium,
 P and albumin levels will be tested. Calculation of CA will be performed. Subjects will
 provide the container with 24 hour urine collection for Ca, P and creatinine to test
 calciuria. Subjects will be asked about any side effects or AEs that may have occurred and
 changes in concomitant medications since their last visit. Subjects will be asked about
 symptoms and signs related with a change in serum calcium levels (tetany, facial grimacing,
 paresthesias, muscle aches, arrhythmia, depression).

 Subjects previously assigned to the CCS treatment arm will receive a pack of ACC tablets,
 each tablet containing 200 mg elemental calcium (35 day supply + 5 spare tablets). Subjects
 previously assigned to the ACC treatment arm, will be instructed to resume their regular CCS
 supplementation.

 The exact dosage of calcium supplementation for each subject will be determined by the known
 medical history.

 Subjects will be reminded to continue their routine medications consumption during the trial.

 II-Day 38 (±1): Subjects will arrive at the Clinical Research Center and their serum calcium,
 P and albumin levels will be tested. Calculation of CA will be performed to exclude
 hypocalcaemia (Ca<7.0 mg/dL). If CA levels are within the desired target range (7.0-10.0
 mg/dl), subjects will continue to take the calcium doses that were instructed on II-day 35.
 If CA levels are below 7.0 mg/dl or above 10.0 mg/dl, changes to the calcium intake will be
 made, according to the doctor's decision

 II-Day 42 (±1): Subjects will arrive at the Clinical Research Center and their serum calcium,
 P and albumin levels will be tested. Calculation of CA will be performed. Subjects will be
 asked about any side effects or AEs that may have occurred and changes in concomitant
 medications since their last visit. Subjects will be asked about symptoms and signs related
 with a change in serum calcium levels (tetany, facial grimacing, paresthesias, muscle aches,
 arrhythmia, depression). Adjustments of ACC or CCS intake will be performed if necessary.

 Subjects will be reminded to take the calcium supplementation according to their assignment
 instructions.

 Subjects will be reminded to continue their routine medications consumption during the trial.

 II-Day 45 (±1): Subjects will arrive at the Clinical Research Center and their serum calcium,
 P and albumin levels will be tested. Calculation of CA will be performed to exclude
 hypocalcaemia (Ca<7.0 mg/dL). If CA levels are within the desired target range (7.0-10.0
 mg/dl), subjects will continue to take the calcium doses that were instructed on II-day 42.
 If CA levels are below 7.0 mg/dl or above 10.0 mg/dl, changes to the calcium intake will be
 made, according to the doctor's decision

 II-Day 49 (±1): Subjects will arrive at the Clinical Research Center and their serum calcium,
 P and albumin levels will be tested. Calculation of CA will be performed. Subjects will be
 asked about any side effects or AEs that may have occurred and changes in concomitant
 medications since their last visit. Subjects will be asked about symptoms and signs related
 with a change in serum calcium levels (tetany, facial grimacing, paresthesias, muscle aches,
 arrhythmia, depression).

 Subjects will be reminded to take the calcium supplementation according to their assignment
 instructions.

 Subjects will be reminded to continue their routine medications consumption during the trial.

 II-Day 56 (±1): Subjects will arrive at the Clinical Research Center and their serum calcium,
 P and albumin levels will be tested. Calculation of CA will be performed. Subjects will be
 asked about any side effects or AEs that may have occurred and changes in concomitant
 medications since their last visit. Subjects will be asked about symptoms and signs related
 with a change in serum calcium levels (tetany, facial grimacing, paresthesias, muscle aches,
 arrhythmia, depression).

 Subjects will be reminded to take the calcium supplementation according to their assignment
 instructions.

 Subjects will be reminded to continue their routine medications consumption during the trial.

 Subjects will receive a container to perform 24 hour urine collection prior to their next
 scheduled visit.

 II-Day 69 (±1) by phone: Subjects will be reminded to perform 24 hour urine collection.

 II-Day 70 (±1) - Termination of phase II: Subjects will arrive at the Clinical Research
 Center and their serum calcium, P and albumin levels will be tested. Calculation of CA will
 be performed. Subjects will provide the container with 24 hour urine collection for Ca, P and
 creatinine to test calciuria. Subjects will be asked about any side effects or AEs that may
 have occurred and changes in concomitant medications since their last visit. Subjects will be
 asked about symptoms and signs related with a change in serum calcium levels (tetany, facial
 grimacing, paresthesias, muscle aches, arrhythmia, depression).
Inclusion Criteria:

 -  Primary Hypoparathyroidism - low levels of intact PTH during hypocalcemia at
 diagnosis.

 -  Subjects receiving calcium and vitamin D supplementation at least 1 year prior to the
 beginning of the study.

 -  Subjects able to adhere to the visit schedule and protocol requirements and be
 available to complete the study.

 -  Subjects who provide written informed consent to participate in the study.

 -  Age: 18-80, inclusive.

 Exclusion Criteria:

 -  Calcium (albumin corrected) serum values below 7.0 mg/dL or above 10.0 mg/dL

 -  Any known diseases affecting the absorption from the gastrointestinal tract:

 -  Inflammatory bowel disease (e.g., Crohn's disease, ulcerative colitis)

 -  Chronic diarrhea

 -  Subjects with neuropsychiatric disease.

 -  Subjects with impaired renal function (glomerular filtration rate, ≤40 mL/min)

 -  Subjects with other severe chronic disease requiring long-term therapy.

 -  Impaired liver function (Liver enzymes> x3 upper limit of normal).

 -  Subjects with history or presence of kidney stones

 -  Recurrent urinary tract infections

 -  Subjects taking drugs which might affect calcium levels such as:

 -  Fusid

 -  Anticonvulsants

 -  Carbonic anhydrase

 -  Adrenocorticosteroids

 -  Subjects who are non-cooperative or unwilling to sign consent form.

 -  Pregnant or breast-feeding women.