HIV Accelerated Liver Disease in Uganda
HIV Accelerated Liver Disease in Uganda
Background:

 - Liver disease is a leading cause of death in people who have the human immunodeficiency
 virus (HIV). It especially affects those who have both HIV and hepatitis B or C viruses. Most
 research on HIV-related liver disease has been conducted in North America and Europe.
 However, HIV-related liver disease in Uganda and other African nations may involve other
 diseases that are not common in the West, and may not involve hepatitis B or C. Researchers
 want to study HIV-related liver disease in Uganda to learn more about the differences between
 Western and African trends of this disease.

 Objectives:

 - To study HIV-related liver disease in rural Uganda.

 Eligibility:

 -  Individuals at least 18 years of age who were tested for possible liver disease. Some
 participants will have HIV infection; others will be uninfected.

 -  All participants will be from rural areas of Uganda.

 Design:

 -  Participants will have at least two study visits.

 -  Participants will have a physical exam and medical history. They will complete a
 questionnaire about health and quality of life. Blood, urine, and stool samples will be
 collected. Participants will also have a liver scan to check for liver scarring, and an
 ultrasound to take images of the liver.

 -  Participants who may have liver disease will visit a local hospital for more tests. A
 liver biopsy will be performed to collect liver tissue samples.
With improved survival following the introduction of highly active antiretroviral therapy
 (HAART), liver disease has become a leading cause of death among HIV-infected persons in
 Western cohorts, primarily affecting those co-infected with hepatitis B or C viruses (HBV,
 HCV). However, data are sparse on liver disease in HIV-infected populations from Uganda and
 other African nations, where the etiologies of liver disease are broader and include
 aflatoxin, schistosomiasis and other infectious and environmental agents. Our previous
 noninvasive study in rural, Rakai, Uganda indicates that the prevalence of significant liver
 fibrosis is high among HIV-infected individuals (17%) and is 50% higher than in
 HIV-uninfected persons, although the prevalence of viral hepatitis B co-infection is low
 (5%). The study presented here is a biopsy-based study that follows up on these results with
 the objectives of defining the etiology of liver disease and describing the mechanisms of
 HIV-accelerated liver fibrosis in this setting.
-  INCLUSION CRITERIA:

 1. Adults aged 18 and older

 2. Persons who are able and willing to provide informed consent

 3. Persons who have a transient elastography score >=9.0 kPa or have other evidence
 of liver disease, and who are willing to undergo an ultrasound and liver biopsy

 i) Other evidence of liver disease: Persons with an LSM <9.0 kPa who demonstrate
 liver transaminases at least 2-times greater than the upper limit of normal;
 those with persistently abnormal liver

 transaminases over a period of three months during the year preceding enrollment;
 those who show abnormalities on their ultrasound; those with other laboratory
 tests indicating the possibility of liver disease; and/or those who have a
 clinical or medical indication for a liver biopsy.

 4. Persons who are willing to have tissue samples undergo genetic testing

 5. Persons who agree to have samples stored for the purpose of future research

 EXCLUSION CRITERIA:

 1. Women who are pregnant

 2. Persons with a cardiac device (i.e., pacemaker)

 3. Participants who are not able to follow study instructions

 4. Safety laboratory data indicating possible excess risk of bleeding including platelets
 <75,000 and an INR>=1.5. These safety laboratory values will be ascertained by
 obtaining a Complete Blood Count (CBC) and a Prothrombin Time (PT).

 5. Evidence of decompensated liver disease including ascites, or hepatic encephalopathy

 6. Persons who have any condition deemed, by the investigators, to be a contraindication
 to study participation