Endometrial Cancer and Array CGH
Endometrial Cancer and Array CGH
Medical context:

 Several molecular studies showed chromosomal alterations in patients with endometrial cancer,
 with gains in 1q, 19p, 19q, 8q, 10q and 10p and loss of 4q, 16q and 18q. Several genes of
 interest have been dentified (P53, PTEN, PIK3CA, ß-catenin, K-ras ...). It is thus
 conceivable that like that enable genomic tools used in breast cancer today (Oncotype DX,
 MammaPrint), correlation between the tumor profile and life project in the case of cancer the
 endometrium could be done. A study is already underway at the Reims University Hospital with
 funding from the League against cancer and AOL in 2010 CHU Reims. It should identify the
 specific alterations of nosologic continuum of pathology and characterize areas of interest
 on the genome. To date, 39 patients with endometrial cancer and 15 patients with endometrial
 hyperplasia (patients 'cases') were included in the study. For this study, 10 patients
 exhibiting neither cancer nor endometrial hyperplasia were also included. Samples of these 10
 patients 'witnesses' were pooled to serve as a reference for analyzing patients 'cases'.

 objectives: In continuation of the study began in November 2009, refine the study of genomic
 imbalances highlighted hyperplasia and endometrial cancer by studying the in correlation
 between genomics and proteomics by immunohistochemical studies and analyzing the
 microsatellite instability.

 To study the prognostic role of genetic factors in patients carriers of a disease endometrial
 (cancer or hyperplasia).

 Material and methods :

 Experimental Design: Cross-sectional study in inclusion prospective multi-center followed by
 a cohort study of patients 'cases'.

 Population / patients: in total, it is planned to include 72 patients with hyperplasia or
 endometrial cancer (22 patients included in the pre-study part funded by the League against
 cancer 50 patients included in the scope of the study funded by the 2010 AOL Chu Reims).

 Plan of investigation: the study includes two phases:

 Sectional study:

 The inclusion of 10 patients "witnesses" has already been completed in the first project.

 During the consultation in the obstetrics and gynecology department of the Reims University
 Hospital and the surgical department of the institute Jean Godinot, terms and objectives of
 the study have been and will be presented to patients where cancer or hyperplasia endometrial
 was diagnosed by achieving endometrial biopsy.

 If the patient agrees to participate in the study, the management of its pathology
 (hysterectomy, hysteroscopy and curettage resection) will not be changed.

 The samples taken during the surgery were analyzed and will be as provided in the first
 research project. Tumor karyotype, DNA extraction are performed on each sample fresh. A
 comparative genomic hybridization is conducted using the DNAs thus obtained.

 Additional analyzes (immunohistochemistry and analysis of microsatellite instability) will be
 performed on all samples already obtained and on future withdrawals.
Inclusion Criteria:

 -  Having a pathological diagnosis of hyperplasia or endometrial cancer

 -  consent to participation in the study

 Exclusion Criteria:

 -  Not affiliated to a social security scheme