Effects of Dietary Carbohydrate and Fat on Hormones
Effects of Dietary Carbohydrate and Fat on Hormones
This study will try to determine if intake of dietary fats or carbohydrates influences the
 tendency to gain weight or accumulate body fat. It will examine how the hormones that
 regulate weight may change with a shift from a balanced diet to one that is low in fat or
 carbohydrate, irrespective of caloric intake.

 Men and women between the ages of 18 and 40 years with a body mass index of 30 to 49 kg/m2
 may be eligible for this study. Candidates will be screened with a history, physical
 examination, blood tests and questionnaires regarding activity, prior diets, and eating
 patterns.

 Participants will follow two separate diet regimens for 5 days each. The first will be a
 balanced diet, and the second will be either a low-fat/high-carbohydrate diet or a
 low-carbohydrate/high-fat diet. The diets are not designed to produce weight loss.
 Participants will receive all their meals and snacks for the two diets from the National
 Institutes of Health and, for the first 3 days of each diet, will be seen as outpatients. For
 days 4 and 5 of each diet, they will be admitted to the Clinical Center and will undergo the
 following procedures:

 -  Blood tests - Blood samples will be collected every 30 minutes over a 24-hour period
 through an indwelling catheter in the arm. A total of about 15 tablespoons will be drawn
 for each of the two diets.

 -  24-hour urine collection - One 24 hour urine collection will be done at home the day
 before admission to the Clinical Center, and the other will be done during the hospital
 stay.

 -  Urinalysis and pregnancy test - A small urine sample will be taken and women will
 provide an additional sample for a pregnancy test.

 -  Body measurements - Height and weight will be measured using scales. Body fat will be
 measured by two methods: skin fold thickness, measured with calipers in 5 separate
 places; and body circumference measurements, in which body parts are measured with a
 tape measure.

 -  Bod pod measurement - Proportions of fat and non-fat tissue in body weight are measured
 while the participant sits in a capsule-like device for 5 minutes.

 -  Bioelectric impedance analysis - Proportions of body fat are measured based on
 conduction of a small electric current.

 -  Total body water estimate -The proportion of body weight that is water is determined,
 using a method that involves drinking a cup of a special kind of "heavy" water
 (deuterium) and then measuring the amount of heavy water in a urine sample taken after 4
 hours.

 -  Exercise - Participants will maintain their usual exercise routine, as it was before
 beginning the study.
Caloric restriction triggers a number of homeostatic endocrine responses including
 suppression of leptin, gonadotropins, sex steroids, thyroid hormones, insulin and insulin
 like growth factors (IGFs) and elevation of growth hormone (GH) and cortisol. Some of these
 effects may be disadvantageous in terms of achieving loss of adipose tissue. There is little
 information on the role of specific macronutrients in the regulation of these important
 endocrine responses to food restriction. The present study aims to determine if dietary
 carbohydrate and/or lipid composition has significant effects on these hormonal regulators of
 body composition and metabolism, independent of total calorie supply. This will be achieved
 by comparing the short-term effects of isocaloric, weight maintenance diets restricted in
 either fat or carbohydrate with a standard balanced diet. Protein contribution as well as
 calorie content will be held constant in the 3 diets. Study subjects will be obese women and
 men (BMI 30-34.9 kg/m2) aged 18-40 years. Major study outcome parameters include: leptin,
 gonadotropin, GH and cortisol secretory dynamics; sex steroid, IGF, fasting insulin and
 glucose levels.
INCLUSION CRITERIA:

 Ages 18-40.

 Men and women.

 BMI 30-34.9 kg/m2.

 EXCLUSION CRITERIA:

 Major medical or psychiatric diagnoses active within the past 3 years.

 Endocrine diseases like, thyroid disease, DM, pituitary, ovarian/testicular and adrenal
 problem

 Acute infections

 Irregular menstrual cycles (greater than 7 day menses, intervals greater than 35 or less
 than 21 days)

 A weight change of greater than 2% within the past 2 months.

 Use of prescription medications or other drugs/supplements including acetaminophen or
 alcohol that would, in the judgement of study investigators, interfere with study
 objectives

 Major (greater than 3 hours/week) athletic/physical exercise activities.

 Eating patterns or dietary preferences which would hinder compliance with study diets

 Tran meridian travel within 2 weeks preceding study periods

 Inability to comply with the study protocol

 Pregnancy

 Anemia. Hemoglobin less than 11.5 for women and less than 12.7 for men.