Inflammatory Mediators and microRNA Analysis in Premenstrual Asthma
Inflammatory Mediators and microRNA Analysis in Premenstrual Asthma
A small number of women with asthma have an increase in asthma symptoms during the
 premenstrual or menstrual period. This study is being done to investigate the possible causes
 of this premenstrual increase in asthma symptoms and to help asthma care providers identify
 those patients who may suffer from this condition.
It is well established that women suffer more asthma symptoms and worse health-related
 quality of life than men with the same level of asthma severity. The etiology for these
 sex-related differences in unknown, but a subset of women has premenstrual asthma (PMA) with
 worsening of their asthma symptoms either prior to or during menstruation. Previous small
 trials have suggested that an increase in the host inflammatory response may correlate with
 PMA symptoms. We have evidence that small inhibitory ribonucleic acids, microRNAs,
 circulating in the peripheral blood of human patients may be expressed in different patterns
 in certain disease states when compared to healthy individuals.

 We plan to compare the patterns of microRNA expression in a well characterized group of women
 with PMA to those without PMA to determine if alterations in these microRNA patterns play a
 role in increased asthma symptoms in the premenstrual period. In addition, we plan to compare
 the levels of inflammatory markers in these populations to better define the specific subset
 of women that may be at risk for premenstrual asthma. By better characterizing these women,
 we hope to identify clinically relevant predictors that may guide therapy for women who
 suffer from PMA.

 Procedures to be used in this study include questionnaire administration, pulmonary function
 assessment, exhaled nitric oxide evaluation, urine sampling for pregnancy, and blood draws
 for microRNA and serum leukotriene evaluation. These techniques are utilized widely in
 clinical asthma research, and are associated with minimal risk.
Inclusion Criteria:

 -  Diagnosis of asthma

 Exclusion Criteria:

 -  Are pregnant

 -  Take hormonal medication

 -  Have an illness with fever (> 38.0 ˚ C or 100.4 ˚ F) within 24 hours of Visit 1 or
 between Visits 1 and 2

 -  Are participating in another interventional research trial

 -  Have other major chronic illnesses that would interfere with participation

 -  Are taking the following medications: leukotriene receptor antagonists, oral steroids,
 anticoagulants, or insulin

 -  Are unable to provide consent