Bacteriophage Effects on Pseudomonas Aeruginosa
Bacteriophage Effects on Pseudomonas Aeruginosa
Pulmonary phage therapy to treat bacterial infections of the respiratory tract have been
 investigated in animals. The aim of the present study is to evaluate the efficacy of
 bacteriophages in infecting Pseudomonas aeruginosa (PA) strains present in sputum samples. A
 cocktail of 10 bacteriophages will be applied on 60 sputum samples obtained from cystic
 fibrosis (CF) patients during 6 hours.We will determine the bacteria and bacteriophages
 strains in sputum samples collected. Then the sensitivity of individual colony will be
 tested.
Inclusion Criteria:

 -  Informed consent

 -  Affiliated or benefit from a disease insurance regimen

 -  Men and women

 -  Aged from at least 6 years old

 -  Confirmed Diagnosis of cystic fibrosis based on presence of 2 mutations of CFTR gene
 and/or 2 positive tests of sweat chloride and/or 2 measures of pathologic difference
 of nasal potential associated to cystic fibrosis clinical signs.

 -  Patients able to produce sputum

 -  Pseudomonas aeruginosa Chronic infected patients

 Exclusion Criteria:

 -  Simultaneous participation to another project on anti-infection, anti-inflammatory or
 modificating agents

 -  Subjet in exclusion period

 -  Law protected patient

 -  Realisation of sputum production is contra-indicated