Biomarker Assay Validation in Healthy Smokers and COPD Smokers and Ex-smokers
Biomarker Assay Validation in Healthy Smokers and COPD Smokers and Ex-smokers
This study will collect sputum samples from healthy smokers, COPD smokers and COPD ex-smokers
 to analyse biomarkers of inflammation
Inclusion Criteria for Healthy Smoking Subjects

 1. Must have signed an informed consent indicating that they understand the purpose of
 and procedures required for the study and are willing to participate in the study.

 2. Be between 18 and 75 years of age, inclusive, at informed consent.

 3. Healthy as determined by a physician, based on medical history and physical
 examination.

 4. Must have smoked regularly in the 12-month period preceding the screening visit and
 have a pack history of ≥ 5 pack years (number of pack years = number of cigarettes per
 day/20 x number of years smoked).

 Inclusion Criteria for All COPD Subjects

 1. Must have signed an informed consent indicating that they understand the purpose of
 and procedures required for the study and are willing to participate in the study.

 2. Aged between 40 and 75 years of age inclusive, at the time of signing the informed
 consent.

 3. COPD diagnosis: Subjects with a diagnosis of COPD as defined by the American Thoracic
 Society (ATS)/European Respiratory Society (ERS) guidelines (Celli, 2004). Symptoms
 must be compatible with COPD for at least 1 year prior to screening and
 post-bronchodilator spirometry readings at screening:

 -  Post-bronchodilator FEV1/FVC ratio of <0.7

 -  Post-bronchodilator FEV ≥40 % and ≤80 % of predicted normal values calculated
 using NHANES reference equations.

 Additional Inclusion for Smoking COPD Subjects 1. Must have smoked regularly in the
 12-month period preceding the screening visit and have a pack history of ≥ 5 pack years
 (number of pack years = number of cigarettes per day/20 x number of years smoked).

 Exclusion Criteria for Healthy Smoking Subjects Any potential subject who meets any of the
 following criteria will be excluded from the participating study.

 1. Upper or lower respiratory tract infection within 4 weeks of the screening visit.

 2. Positive test for alcohol at screening.

 3. Taking prescription medication in the 14 days before screening.

 4. Subjects whose primary consumption of tobacco is via methods other than cigarettes
 (manufactured or self-rolled). Primary methods of tobacco consumption that are
 excluded include, but are not limited to pipes, cigars and e-cigarettes.

 5. Subjects who are unable to produce a total weight of at least 0.1 grams (g) of
 selected sputum at screening

 6. Urinary cotinine levels at screening < 30 ng/ml.

 7. Subject is mentally or legally incapacitated.

 8. Subject is an employee of the Sponsor or contract research organization (CRO), or a
 relative of an employee of the Sponsor or CRO.

 9. Any other reason that the Investigator considers makes the subject unsuitable to
 participate.

 Exclusion Criteria for COPD Subjects Any potential subject who meets any of the following
 criteria will be excluded from the participating study

 1. Upper or lower respiratory tract infection within 4 weeks of the screening visit.

 2. Positive test for alcohol at screening.

 3. Subjects who are unable to produce a total weight of at least 0.1g of selected sputum
 at screening.

 4. Subject is mentally or legally incapacitated.

 5. Subject is an employee of the Sponsor or contract research organization (CRO), or a
 relative of an employee of the Sponsor or CRO.

 6. Any other reason that the Investigator considers makes the subject unsuitable to
 participate.

 7. A history of life-threatening COPD including Intensive Care Unit admission and/or
 requiring intubation.

 8. Taking theophylline in the 28 days before screening

 9. Change in COPD medication in the 28 days before screening

 10. Taking oral steroids in the 28 days before screening

 Additional Exclusion Criterion for Smoking COPD Subjects

 1. Subjects whose primary consumption of tobacco is via methods other than cigarettes
 (manufactured or self-rolled). Primary methods of tobacco consumption that are
 excluded include, but are not limited to pipes, cigars and e-cigarettes.

 2. Urinary cotinine levels at screening < 30 ng/ml.

 Additional Exclusion Criterion for Ex-Smoking COPD Subjects

 1. The subject is a smoker (regular or irregular), or has smoked or used
 nicotine-containing products within the 6 months prior to screening.