Characteristics of Lower Respiratory Tract Escherichia Coli Isolates in Mechanically Ventilated Intensive Care Patients
Characteristics of Lower Respiratory Tract Escherichia Coli Isolates in Mechanically Ventilated Intensive Care Patients
Prospective, multicenter observational study to collect Escherichia coli (E. coli) isolates
 originating from mechanically ventilated intensive care unit (ICU) patients; in order to
 characterize phenotype and genotype of E. coli strains retrieved from the lower respiratory
 tract of ventilated patients.
Prospective, observational, multiple center study performed in 14 ICUs in France to collect
 Escherichia coli (E. coli) isolates originating from mechanically ventilated intensive care
 unit (ICU) patients; in order to characterize phenotype and genotype of E. coli strains
 retrieved from the lower respiratory tract of ventilated patients. All E. coli isolates
 identified in the microbiology lab and retrieved from a lung specimen (either tracheal
 aspirate, bronchoalveolar lavage, or telescopic plugged catheter) originating from an ICU
 patient will be kept, and stored at -80°C in brain-heart infusion broth containing glycerol
 20 %. They will be then centralized in the investigators' research unit for further analysis
 that includes determination of Antimicrobial susceptibility, E. coli phylotype , O-type, and
 virulence factor gene content.

 These isolates will be compared to those of two previously published collections, one from
 the stools of community subjects, considered as commensal strains, the other from the blood
 of bacteraemia patients.
Inclusion Criteria:

 -  adult, admitted to the intensive care unit

 -  under invasive mechanical ventilation

 -  presence of Escherichia coli in lower respiratory tract specimen

 Exclusion Criteria:

 -