Eradication of Gut Microbiota
Eradication of Gut Microbiota
The aim of the study is to assess the effect of eradication of gut microbiota on 1) glucose
 metabolism including postprandial plasma responses of the incretin hormones GIP and GLP-1,
 insulin, C-peptide and glucagon, 2) metabolomic profiles and resting energy expenditure (REE)
 3) appetite, satiety, food intake, gastric emptying and gall bladder emptying, 4) levels of
 markers of bone formation and resorption as well as serotonin, 5) markers of systemic
 inflammation, and 6) on the (prospective) composition of bacteria in faeces, blood and
 saliva. Thus, the overall objective is to provide detailed knowledge on the physiological
 role of gut microbiota combined with bioinformatic analyses of the functional implications of
 changes in bacteria composition on the level of both species and phylum.
Inclusion Criteria:

 -  danish caucasian ethnicity

 -  informed consent

 -  normal fasting plasma glucose

 -  normal HbA1c (<6 %)

 -  normal serum lipids

 -  normal thyroid function

 -  normal danish diet

 -  non-smoking

 -  normal stool habits

 Exclusion Criteria:

 -  known bone disease

 -  liver disease (ALAT or ASAT >2 upper normal value)

 -  kidney disease (serum creatinine >130 μM)

 -  anaemia

 -  BMI <18.5 kg/m2 or BMI >25 kg/m2

 -  known gastrointestinal disease (including prior bariatric surgery,lactose
 -intolerance, celiac disease, inflammatory bowel disease) or known familial
 disposition for lactose intolerance, celiac disease, inflammatory bowel disease

 -  antibiotic treatment within 6 months prior to study including malaria prophylaxis

 -  medication which cannot be on hold for the study period

 -  contraindications against/allergy towards the used antibiotics (including prior
 allergic reactions related to beta-lactam antibiotics, aminoglycosides or vancomycin)