Maternal Singing During Kangaroo Care in Preterm Infants
Maternal Singing During Kangaroo Care in Preterm Infants
Study objective is to evaluate the additive effect of maternal singing during skin to skin
 contact (Kangaroo Care) on anxiety reduction both to infants and their mothers.
Maternal singing to preterm infants during Kangaroo Care intervention, is it beneficial?

 Protocol

 Maternal voice is a predominant source of multimodal stimulation for the developing fetus
 that is largely lost for the preterm infant amidst the unfiltered levels of auditory stimuli
 in the neonatal intensive care unit (NICU). These elevated levels of auditory stimulation in
 the NICU can interfere with an infant's sleep and have a negative effect on vital signs and
 oxygen saturation. Common sources of sound for the preterm infant are derived from routine
 incubator noise, monitoring alarms, and the speech sounds of healthcare team, all of which
 they would not typically encounter if life in the uterus had continued to term. In the midst
 of these additional sounds, preterm infants lose significant contact with an auditory
 stimulus they would typically be exposed to until full-term birth (their mothers' voice).

 Skin to skin contact care (Kangaroo care) was found to produce cardiorespiratory stability,
 decrease apneic and periodic breathing, and improve sleep patterns and weight gain in preterm
 infants. This technique has also been shown to help mothers of preterm infants to develop and
 strengthen self-confidence and attachment behaviors, leading to reduced stress response. Live
 music was shown to have a beneficial effect on psychophysiological responses in both mothers
 and preterm infants during their stay in the NICU.

 Aim: To evaluate the combined influence of live maternal singing during kangaroo care (KC) on
 maternal anxiety and stable preterm infants' responses.

 Participants

 Inclusion criteria clinically stable infants

 postmenstrual age <37 weeks

 normal hearing confirmed by measurement of distortion product otoacoustic emission (DPOAE).

 Exclusion criteria

 observed hyperresponsiveness to live music

 congenital anomaly that mainly affects hearing

 medication intake (phenobarbital, furosemide, gentamycin) that might interfere with the
 reaction to musical stimuli

 brain anomalies associated with neurological disorders (grade 3-4 intraventricular
 hemorrhage, periventricular leukomalacia).

 Maternal inclusion criteria

 ability to hear

 literary ability to read and answer an anxiety questionnaire

 Maternal exclusion criteria

 signs or medical history of postpartum depression.

 Procedure A within-subject, crossover, repeated design will be used with participants acting
 as their own controls. Before starting the research, mothers will get a professional guidance
 of singing lullabies to their offspring, by a music therapist (E.G). The mothers will be
 sitting at the infants' bedside and the child will be placed in the kangaroo position with
 skin-to-skin contact with the mother. Each therapy session will start 30 minutes after
 feeding to get a relaxed infant and to test the intervention with no disturbances such as
 hunger. As recommended the infants will receive either KC alone for 30 minutes or KC combined
 with maternal singing for 30 minutes. According to the randomization schedule, both therapies
 will be performed in the afternoon after finishing the medical and nursing rounds, in
 alternating order 3 to 5 days apart to allow for a washout period, and included the same
 music. Monitor alarms will be silence and the door closed.

 Objective Sound Measurements Music and background sound levels will be checked 10 cm from the
 infant's ear with a sound analyzer and a decibel scale filter (407790 Octave Band Sound
 Analyzer, type 2 Integrating Sound Level Meter and Decibel-A Scale Filter, respectively;
 Extech Instruments, Melrose, MA, USA).

 Infant and Maternal Measures For accuracy, neonatal and maternal heart rates, oxygen
 saturation levels, and respiratory rates will be recorded continuously and documented every 2
 minutes during all sessions. A 7-point scale to assess infants' behavioral state (deep sleep,
 light sleep, drowsy, quiet awake or alert, actively awake and aroused highly aroused, upset,
 or crying, and prolonged respiratory pause > 8 seconds) and a self-report questionnaire
 comprising 20 descriptive statements for measuring maternal anxiety symptoms (state-trait
 anxiety inventory [STAI] scale will be used at the beginning and the end of each
 intervention. Higher scores indicate greater anxiety, with a possible score of 20 to 80
 points. Additional maternal measures will be age, ethnicity (Arab/Jews), education (8 years,
 elementary school; 8-12 years, high school; and >13 years, college/university degree), and
 affinity for music (a 5-point scale: 1, 2, 3, 4, and 5 hours, measuring the length of time
 spent a day listening to music).

 Data will be recorded by a single physician who will remain at the bedside throughout each
 session. Data will be analyzed in a blinded fashion another researcher who will be unaware of
 treatment allocations.

 Power Analysis:

 To achieve a power of 0.8 at α = 0.05 and one-tailed with a medium effect size of 0.610 and a
 medium correlation (r = 0.50) among two repeated measures using the F-test, a sample size of
 30 mother-infant dyads will be needed.
Inclusion criteria clinically stable infants

 -  postmenstrual age <37 weeks

 -  normal hearing confirmed by measurement of distortion product otoacoustic emission
 (DPOAE).

 Exclusion criteria

 -  observed hyperresponsiveness to live music

 -  congenital anomaly that mainly affects hearing

 -  medication intake (phenobarbital, furosemide, gentamycin) that might interfere with
 the reaction to musical stimuli

 -  brain anomalies associated with neurological disorders (grade 3-4 intraventricular
 hemorrhage, periventricular leukomalacia).

 Maternal inclusion criteria

 -  ability to hear

 -  literary ability to read and answer an anxiety questionnaire

 Maternal exclusion criteria

 -  signs or medical history of postpartum depression