A Laser Detection for Bladder Cancer by (Photodynamic) Spectra of Urine
A Laser Detection for Bladder Cancer by (Photodynamic) Spectra of Urine
Research Problem:

 Bladder cancer is one of the major health concerns of the world. The present methods of
 diagnosis are: Ultra sound, Cystoscopy, CT scan and urine cytology. All these are stressful
 to the patients, particularly Cystoscopy which is commonly employed for the follow up of
 Bladder cancer patients.

 Research Significance:

 The present study will employ a new photodynamic diagnostic procedure to quantify a certain
 cancer specific biomarker called Porphyrin, which selectively binds on to the bladder cancer
 tissues. In this context the present technique offer viable, very easy and reliable table top
 instrumentation for diagnosis and continual monitoring of disease regression through urine.

 Research Objectives:

 -  To quantify bladder cancer specific biomarkers such as Porphyrin using photodynamic
 diagnostic procedure

 -  To find out whether this technique might be a new and easy tool for bladder cancer
 diagnosis only by urine.

 Research Methodology:

 The bladder cancer patients is required to swallow a chemical called ALA (5 Amino levulinic
 Acid hydrochloride), about 10mg/kg body weight which will play a role of biological
 indicator. ALA gets metabolized into certain types of porphyrins which selectively bind on to
 the tumor tissues (for a longer time than the normal tissues). 5ml of blood and one urine
 samples will be taken before using ALA. The patient must drink water then the urine will be
 collected after 4, 8 and 12 hours of taking ALA and the samples will be analyzed by
 photodynamic diagnostic procedure.
Bladder cancer is one of the most common urologic cancers in Saudi Arabia. its cases
 represent a significant and challenging part in the daily practice for the majority of
 urologists practicing. The present methods of diagnosis are: Ultra sound, Cystoscopy, CT-scan
 etc. All these are stressful to the patients, particularly Cystoscopy which is commonly
 employed for the follow up of Bladder cancer patients.

 In recent years, fluorescence cystoscopy, in contrast to conventional white light cystoscopy,
 has been investigated as a tool to enhance detection of occult papillary lesions and
 carcinoma in situ. Recent fluorescence photo detection strategies have used 5-aminolevulinic
 acid (5-ALA); a precursor of heme biosynthesis.

 5-Aminolevulinic acid-enhanced cystoscopy does appear to have improved sensitivities in
 detecting nonmuscle invasive bladder tumors such as carcinoma in situ . Aminolevulinic acid
 (ALA) may have a role in other applications in surgical oncology based on its ability to
 discriminate neoplastic tissue from adjacent normal tissue. Aminolevulinic acid is not a
 photosensitizer, but rather a metabolic precursor of porphyrin, which is a photosensitizer.

 The present study will employ photodynamic diagnostic procedure to quantify only in urine a
 specific cancer biomarker Porphyrin which selectively binds on to the bladder cancer tissues
 for a longer time than the normal tissues. In this context, the present study will offer
 viable, very easy and reliable table top instrumentation for diagnosis and continual
 monitoring of disease regression.
Inclusion Criteria:

 -  Patients: Urothelial (transitional cell) carcinoma. Early and advanced stages of with
 transitional cell carcinoma of the bladder before transurethral resection of bladder
 Tumor (TURBT) .

 -  Controls: Healthy volunteer's adults matched with age.

 Exclusion Criteria:

 -  Urinary tract infection (UTI) patients

 -  Cardiac patients