Multi-4SCAR-T Therapy Targeting Breast Cancer
Multi-4SCAR-T Therapy Targeting Breast Cancer
The purpose of this study is to assess the feasibility, safety and efficacy of multiple 4th
 generation CAR-T cells targeting Her2, GD2, and CD44v6 surface antigen in breast cancer.
 Another goal of the study is to learn more about the activities of the multi-CAR T cells and
 their persistency in the patients.
Breast cancer is one of the most frequent cancer types in women. The average risk of a woman
 in the United States developing breast cancer in her life is about 13%. That means that there
 is a 1 in 8 chance she will develop breast cancer. Human epidermal growth factor receptor-2
 (HER2) is one of the more well-researched genes in breast cancer so far. It has been reported
 that HER2 gene is overexpressed in 20% to 30% of breast cancer patients. HER2 is an important
 target for tumor gene therapy. In 2003, scientists confirmed the existence of breast cancer
 stem cells. In 2012, ganglioside GD2 was confirmed as an emerging marker of breast cancer
 stem cells. Targeting therapies for GD2 may help improve the survival rate and cure rate of
 breast cancer patients. Invasion and metastasis of tumor cells is the main cause of cancer
 death. CD44v6 is an adhesion molecule on the cell surface, which not only promotes
 epithelial-mesenchymal transition, degradation and remodeling of extracellular matrix, but
 also participates in organ-specific metastasis of tumor cells. Studies have shown that
 overexpression of CD44v6 is an important factor for the invasion and metastasis of breast
 cancer, and is closely related to the tumor size of the breast cancer, tumor staging, and
 lymph node metastasis. Therefore, CD44v6 may be an important target for the treatment of
 breast cancer. Using Her2-, GD2- and CD44v6-specific CAR-T cells may effectively improve the
 immunotherapy treatment, prevent tumor cells from escaping treatment, and achieve the effect
 of long-term disease relief.
Inclusion Criteria:

 1. Patients with stage III, IV or relapsed breast cancer confirmed by histology and
 biopsy.

 2. Age: ≥ 18 years and ≤ 75 years.

 3. 2 weeks at least since last chemotherapy or radiotherapy and 2 weeks at least since
 last systemic steroid hormone and other immunosuppressive therapy.

 4. Side effects of chemotherapy have subsided.

 5. The target antigens GD2, CD44v6, or Her2 is expressed in malignancy tissues by
 immuno-histochemical or flow cytometry.

 6. Eastern Cooperative Oncology Group (ECOG) PS of 0 or 1.

 7. Expected survival ≥ 12 weeks.

 8. Initial hematopoietic reconstitution with neutrophils (ANC) ≥ 1×10^6/L; platelet (PLT)
 ≥ 1×10^8/L.

 9. Proper renal and hepatic functions (ULN denotes "upper limit of normal range") with
 serum creatinine ≤ 2×ULN; serum bilirubin ≤ 3×ULN; AST/ALT ≤ 2.5×ULN.

 10. Oxygen saturation ≥ 90%.

 11. Written, informed consent obtained prior to any study-specific procedures.

 Exclusion Criteria:

 1. Airway obstruction caused by tumor.

 2. History of epilepsy or other central nervous system diseases.

 3. Patients who require systemic corticosteroid or other immunosuppressive therapy.

 4. History of prolonged or serious heart disease during QT.

 5. Current or recent treatment (within the 28-day period prior to Day 0) with another
 investigational drug or previous participation in this study.

 6. Inadequate liver and renal function with serum creatinine > 1.5 mg/dl; serum (total)
 bilirubin > 2.0 mg/dl; AST & ALT > 3 x ULN.

 7. Pregnant or lactating females.

 8. Serious active infection during screening.

 9. Active HIV, hepatitis B virus (HBV), hepatitis C virus (HCV) infection or uncontrolled
 infection.

 10. Patients, in the opinion of investigators, may not be eligible or not able to comply
 with the study.