Potential Biomarkers of the Severity of Endothelial Damage in End-stage Chronic Kidney Failure
Potential Biomarkers of the Severity of Endothelial Damage in End-stage Chronic Kidney Failure
The principal objective of this study is to qualify markers of oxidative stress in
 inflammatory cells (monocytes) in patients with stage 3 kidney failure (diabetic or not), and
 patients with end-stage kidney failure (diabetic or not), who require dialysis. The
 evaluation of these markers will be done by the activation and localization of proteins
 implicated in vascular tone and oxidative stress in monocytes, correlated with the
 distribution of cholesterol sphingomyelin within planar rafts and caveolae. The aim is to
 describe their evolution under treatment, which could lead to interventional studies.
Inclusion Criteria:

 -  Patient who have been informed about the research

 -  Patients with national health insurance cover

 -  Patients aged between 18 and 75 years

 -  Patients with stage 3 kidney failure (30 ml/min < cl creat < 59 ml/min) diabetic or
 not

 -  Patients with stage 5 kidney failure (cl creat < 15 ml/mn) diabetic or not and
 requiring dialysis.

 Exclusion Criteria:

 -  Patients under guardianship

 -  Pregnant or breast-feeding women

 -  Infection (including peritonitis in peritoneal dialysis, infection of the catheter
 insertion site),

 -  Neoplastic disease,

 -  Systemic diseases in flare,

 -  Patients positive for Human Immunodeficience Virus (HIV),

 -  Patients on immunosuppressants

 -  Patients taking antioxidants (selenium, vitamin C and/or E)

 -  Patient on statins