One Year Study to Evaluate Three Different Adjuvanted Doses of the Recombinant Plague Vaccine (rF1 and rV Antigens)
One Year Study to Evaluate Three Different Adjuvanted Doses of the Recombinant Plague Vaccine (rF1 and rV Antigens)
One hundred and five subjects will be recruited into three groups. Each subject will receive
 two doses of recombinant plague vaccine at one of three dose levels (rF1 and rV recombinant
 antigen proteins).
Plague is an infection occurring in small rodents and mammals caused by the gram-negative
 bacterium Yersinia pestis (Y. pestis). Transmission from rodent to man is usually by a flea
 vector leading to the characteristic swelling of the lymph nodes draining the region of the
 bite, followed by a septicaemic illness (classic bubonic plague). Human-to-human transmission
 can occur via droplet nuclei spread by coughing of patients with bubonic or septicaemic
 plague who have developed pulmonary lesions (pneumonic plague). However, cases of pulmonary
 transmission have also been described from household pets. In pneumonic plague symptoms of a
 respiratory infection develop first followed by an acute onset septicaemic illness. In the
 military context, the likely exposure is via the inhalation route, as a Biological Warfare
 Agent (BWA), and therefore protection against pneumonic plague is the paramount requirement.
 This is a phase 1, parallel group, single-blind study of 105 healthy adult aged 18-55,
 randomly assigned to one of the three cohorts and will receive the same dose of vaccines (2x)
 and then re-randomized at 6 months to receive either a third dose or placebo, in order to
 determine the safety and tolerability associated with different primary immunization doses of
 recombinant IM plague vaccine (rF1 and rV) antigen proteins for optimum safe dose, assess
 responses (both antibody and cell-mediated) following immunization, investigate the
 correlation between cell-mediated and antibody titers and to assess the duration of the
 immune responses to antigens following a third dose.
Inclusion Criteria:

 1. Healthy males or females.

 2. Aged between 18-55 years (inclusive) on Day 0 of the Study.

 3. Of a body mass index (BMI) of 18-35 inclusive.

 4. Able to understand the informed consent form and other documents required to be read
 by the subject.

 5. Willing to give signed informed consent

 6. Able to give a medical history without major organ pathology (e.g. cardiac,
 immunological, psychiatric, endocrine or neurological disorders, cancer or other
 wasting diseases - (adequately treated actinic keratosis, or basal cell carcinoma
 [BCC], or carcinoma in situ [CIS] of the cervix are permitted).

 7. In the case of female subjects, they may be enrolled if one of the following criteria
 applies:

 Either Is not pregnant or breast feeding AND is routinely using adequate injectable or
 transdermal (administered at the recommended frequency) or oral contraception (at a
 stable dose for at least three months prior to the first dose of vaccine) and will
 continue to do so during the study, augmenting this contraceptive measure with a
 barrier method OR is sexually abstinent OR is monogamous and has a partner who has had
 a vasectomy (>1 month previously) OR is using a commonly recognised copper and hormone
 implanted intrauterine device (IUD) such as TCu-380A, TCu-220C, MLCu-375, Nova-T or
 LNG-20. In addition, the subject must have a negative blood pregnancy test prior to
 enrolment into the study (see also Criterion 9 below).

 Or Is post menopausal (defined as a female with no menstrual cycle for at least the
 previous 24 months AND is of menopausal age (>45 years) Or Has not had a menstrual
 cycle for between 12 and 24 months AND is of menopausal age (>45 years) AND has had a
 negative blood pregnancy test prior to enrolment into the study and a negative urine
 pregnancy test pre-dose.

 Or Has been surgically sterilised (confirmed by review of medical record). Or Has had
 a total hysterectomy at least 3 months prior to the start of the study (confirmed by
 review of medical record).

 8. A male may be enrolled if willing to use barrier methods of contraception and whose
 partner is using an acceptable form of contraception for 3 months after each dose.

 9. A female subject must have a negative urine pregnancy test prior to each dosing
 (unless post-menopausal, surgically sterilised, or has had a total hysterectomy, as
 defined in Criterion 7 above).

 10. 12-lead electrocardiogram (ECG) recording without signs of pathology and conduction
 disturbances and with a QTc interval of < 450 msec for males and < 470 msec for
 females. ECGs will be analysed automatically for study entry purposes and will also be
 analysed by a cardiologist within 24 hours. QT intervals will be recorded
 automatically by the ECG machines used.

 Exclusion Criteria:

 1. Presence of any clinically significant medical condition as determined by the
 Investigator.

 2. History of clinically significant hypersensitivity or idiosyncratic reaction related
 to any medical product, including vaccines and aminoglycoside antibiotics (such as
 kanamycin).

 3. History or evidence of drug misuse.

 4. Participation in a clinical study of an investigational vaccine within 3 months prior
 to the start of the study (Day 0) or an investigational drug product within 30 days
 prior to the start of the study.

 5. Use of any prescription or non-prescription medication within 7 days prior to the
 first dosing with the exception of over-the-counter (OTC) antihistamines,
 non-steroidal anti-inflammatory drugs (NSAID) including aspirin, acetaminophen, OTC
 decongestants, herbal medicines (except St. John's Wort), oral/injectable/transdermal
 contraceptives, OTC multi-vitamin preparations (not high-dose preparations) or oral
 iron supplements. Any medication taken within 7 days of the first dosing will be
 documented.

 6. History or suspicion of inability to co-operate adequately.

 7. Donation of blood or blood products during the 4 weeks prior to participation in the
 study (Day 0).

 8. Immunodeficiency or clinically active autoimmune disease.

 9. Positive urine alcohol and/or drug screen for drugs of misuse (opiates, methadone,
 cocaine, amphetamines, cannabinoids, and barbiturates).

 10. Positive serological test for human immunodeficiency virus (HIV), and/or hepatitis B
 virus and/or hepatitis C virus.

 11. Vaccination(s) with a live vaccine in the 4 weeks prior to participation in the study,
 (or 'flu vaccine in the 2 weeks prior to participation), or killed / inactivated /
 sub-unit vaccines in the previous 3 weeks.

 12. Receipt of blood or plasma transfusions, or pooled gamma globulin, in the 3 months
 prior to participation in the study (Day 0) and/or a need for future blood or plasma
 transfusions during this study.

 13. Prior receipt of any plague vaccine.

 14. Prior history of plague infection (confirmed or suspected) or significant exposure to
 Y. pestis (e.g. a laboratory worker who regularly handles Y. pestis) as judged by the
 investigator.

 15. Clinically relevant abnormal findings on routine physical examination.

 16. Clinically significant out-of-range laboratory tests at screening including:
 urinalysis, serum creatinine, serum electrolytes (sodium, potassium, chloride and
 bicarbonate), liver function tests (ALT, AST and GGT), lactate dehydrogenase (LDH),
 white blood cell count, absolute neutrophil count, platelet count, clotting and blood
 haemoglobin. Minor, out-of-range laboratory test results for the above tests may be
 allowable, at the discretion of the Investigator, if such results are within 10% of
 the normal ranges and considered to be not clinically significant.

 17. Twelve-lead ECG recording with clinically relevant signs of pathology and conduction
 disturbances as judged by the investigator.

 18. Presence of tattoos that cover or partially cover the upper arm, and which would limit
 adequate assessment of the injection site.