A Study of Intracellular Signaling in Muscle and Fat Cells During Ketosis
A Study of Intracellular Signaling in Muscle and Fat Cells During Ketosis
Hypothesis

 1. To define whether stimulation of ATGL and suppression of G0/G1 switch gene occur in the
 initial phases of diabetic ketoacidosis and thus can be identified as the primary
 mechanisms behind this life threatening condition.

 2. Make a human model for studying ketoacidosis.

 The investigators plan to reduce in their regular insulin over night. In the morning we
 administer endotoxin, which together with a relative lack of insulin will initiate
 ketogenesis - a state of ketoacidosis. On another occasion strict glycemic control is imposed
 by means of intravenous insulin. The testing is done two separate days with at least 3 weeks
 in between and patients are admitted to hospital the evening before the day of testing. The
 investigators use isotopic tracers to determine metabolic fluxes and analyse fat (ATGL, G0/G1
 switch gene) and muscle biopsies.
Inclusion Criteria:

 -  Diabetes type 1

 -  19 < BMI < 26

 -  minimal or negative C-peptide

 -  written consent

 Exclusion Criteria:

 -  Severe comorbidity

 -  regular medication apart from insulin