Genomic and Proteomic Study of Richter Syndrome (CGPSR)
Genomic and Proteomic Study of Richter Syndrome (CGPSR)
Biological study on Richter Syndrome (RS), an agressive lymphoma that arises from Chronic
 Lymphocytice Leukemia (CLL). RS presents with the same histological aspect as primitive
 Diffuse Large B-Cell Lymphoma (DLBCL), but is associated with a poor prognosis, due to
 chemorefractoriness.

 This study aims at understanding the biological determinants of chemotherapy resistance in
 Richter Syndrome.
With the help of the French National Research Group on CLL (FILO / French Innovative Leukemia
 Organization), the investigators are currently gathering fresh frozen cell pellets at CLL
 stage, and lymph node biopsies at Richter stage. The investigators also gathered lymph node
 biopsies from DLBCL, as a reference group.

 The investigators will perform genomic and proteomic comparative studies between CLL and
 Richter, as well as between Richter and primitive DLBCL, to understand the biological
 determinants of clonal evolution and chemorefractoriness of Richter Syndrom.
Inclusion Criteria:

 -  Diagnosis of a Diffuse Large B-Cell Lymphoma arising in the context of a Chronic
 Lymphocytic Leukemia (group 1) or diagnosis of a primitive Diffuse Large B-Cell
 Lymphoma (group 2), or diagnosis of a Diffuse Large B-Cell Lymphoma arising in a
 context of small cells lymphoma, excluding CLL (group 3), or benefit from a diagnostic
 lymph node biopsy that did not reveal any tumor involvment (primitive or metastatic)
 (group 4).

 -  Patients must benefit from a lymph node biopsy at diagnosis.

 -  Patients must be followed by a FILO (French Innovative Leukemia Organization) member

 -  Histology of Diffuse Large B-Cell Lymphoma or Hodgkin histology.

 -  Suitable clinical data available.

 -  Samples must meet the following requirement :RIN (RNA Integrity Number) > 5 et DIN
 (DNA Integrity Number) > 6.5.

 Exclusion Criteria:

 • Samples that do not meet the inclusion criteria (insufficient clinical data, analysis
 impossible due to insufficient sample quality).