Quantifying Airway Inflammation With Radiologic Tests
Quantifying Airway Inflammation With Radiologic Tests
In this randomized, double-blind, placebo controlled trial we used positron emission
 tomography to determine if lovastatin or recombinant human activated protein C exhibit
 anti-inflammatory effects in humans following intrabronchial installation of
 lipopolysaccharide (LPS or endotoxin).
Quantitative, noninvasive biomarkers for lung-specific inflammation have yet to be developed
 but can potentially contribute significantly to the development of therapies to treat lung
 inflammation. The purpose of this study was to demonstrate that positron emission tomographic
 (PET) imaging with [18F}fluorodeoxyglucose (FDG-PET) can be used to quantify the change in
 lung inflammation in healthy volunteers.
Inclusion Criteria:

 -  Healthy, man or woman, any race or ethnicity, age 19 - 44 years old

 -  Screening FEV1 and FVC must be > 80% of predicted.

 -  Screening oxygen saturation by pulse oximetry is >97% on room air.

 -  Research volunteer must be capable of lying still and supine within the PET scanner
 for ~2 ½ hours.

 -  Research volunteer must be capable of fasting for 6 hours.

 Exclusion Criteria:

 -  Pregnancy (confirmed by a qualitative urine hCG pregnancy test)

 -  Lactation.

 -  Actively menstruating at time of randomization

 -  History of tobacco use or has smoked other illicit drugs (marijuana, cocaine) in the
 past year.

 -  Research volunteer is currently taking any prescription medications.

 -  Research volunteer is at increased risk for radiation exposure (e.g. flight
 attendants)

 -  Research volunteer is enrolled in another research study of an investigational drug.

 -  Research volunteer has a known allergy to both trimethoprim/sulfamethoxazole and
 amoxicillin.

 -  Research volunteer has a known allergy to drugs routinely used during bronchoscopy.

 -  Research volunteer has a known allergy to lovastatin or rhAPC

 -  Fasting glucose at time of PET study > 150 mg/dl.

 -  Exclusion criteria related to use of rhAPC:

 -  Active or history of internal bleeding within the past 3 months

 -  History of hemorrhagic stroke within the past 3 months.

 -  History of intracranial or intraspinal surgery, or severe head trauma, within the
 past 3 months

 -  History of trauma with an increased risk of life-threatening bleeding within the
 past 3 months

 -  History of receiving thrombolytic therapy within the past 3 months.

 -  History of receiving oral anticoagulants or glycoprotein IIb/IIIa inhibitors
 within the past 3 months.

 -  History of using aspirin > 650 mg/d or other platelet inhibitors within the past
 7 days.

 -  Any history of intracranial arteriovenous malformation or aneurysm

 -  Any history of a known bleeding diathesis

 -  Any history of chronic severe hepatic disease

 -  Presence of an epidural catheter

 -  Any history of intracranial neoplasm or mass lesion or evidence of cerebral
 herniation

 -  Use of heparin during past 7 days

 -  Platelet count <100,000 x 106/L

 -  Prothrombin time-INR > 1.5

 -  SGOT >47 IU/L, SGPT > 53 IU/L, or bilirubin > 1.1 mg/dl

 -  Any other condition in which bleeding constitutes a significant hazard or would
 be particularly difficult to manage because of its location.

 -  Exclusion criteria related to use of lovastatin:

 -  History of chronic active liver disease or acute liver disease within the past 3
 months

 -  SGOT >47 IU/L, SGPT > 53 IU/L, or bilirubin > 1.1 mg/dl.