Study Confirming A Human Challenge Model and Investigating The Safety Of VLA1701
Study Confirming A Human Challenge Model and Investigating The Safety Of VLA1701
This is a single-center, double-blind, placebo-controlled, Phase II vaccination and challenge
 study designed to confirm a human challenge model with E. coli strain LSN03-016011/A.
This is a single-center, double-blind, placebo-controlled, Phase II vaccination and challenge
 study designed to confirm a human challenge model with E. coli strain LSN03-016011/A (LT+
 (Labile toxin), ST- (Stable toxin), CS17), as well as collect expanded safety and
 immunogenicity data.

 The study will be carried out in two phases:

 Vaccination phase: up to 34 subjects will be randomized 1:1 to receive 2 doses of either
 VLA1701 or placebo orally. The doses will be given 7 days apart and subjects will be followed
 as an outpatient for safety.

 Challenge Phase: 30 Subjects, out of the 34 subjects, will be challenged.

 After challenge, subjects will be monitored for diarrhea and other signs/symptoms of enteric
 illness by daily medical checks, vital sign determinations, grading and weighing of all
 stools.
Inclusion Criteria:

 1. Healthy male and non-pregnant female subjects aged 18 to <50 years;

 2. BMI of 19.0 to 35.0 kg/m2

 3. Willingness to participate after informed consent has been obtained from the subject
 prior to any study related procedures.

 4. Completion of a training session and demonstration of comprehension of the protocol
 procedures and knowledge of ETEC-associated illness by passing a written examination.

 5. If subject is of childbearing potential:

 1. Negative pregnancy test at screening with understanding to not become pregnant
 within 28 days after challenge;

 2. Subject has practiced an effective method of contraception during the 30 days
 before screening (Visit 0);

 3. Subject agrees to employ adequate birth control measures for the duration of the
 study.

 Exclusion Criteria

 1. Participated in research involving investigational product within 30 days before
 planned date of first vaccination or planned use through Day 44;

 2. Any prior exposure to ETEC (including LSN03-016011/A) or cholera occupationally or
 received LT (Or any mutant forms of LT (e.g., LTR192G, LTR192GL211A), ETEC, or cholera
 vaccine);

 3. Subjects with known abnormal stooling patterns (fewer than 3 per week or more than 3
 per day);

 4. Known allergies to any component of the vaccine;

 5. Subjects with known allergies to more than 1 planned antibiotics:

 6. History of diarrhea while traveling in a developing country within the last 3 years;

 7. Subjects whose occupation involves handling of ETEC or cholera bacteria;

 8. Women who are pregnant or breastfeeding;

 9. Significant medical conditions including chronic, immunosuppressive, malignant, or
 gastrointestinal diseases (e.g. History of Irritable Bowel Syndrome (as defined by the
 Rome III criteria or medical diagnosis) or gastric ulcer disease) or enteric,
 pulmonary, cardiac, liver or renal disease. Some medical conditions which are
 adequately treated and stable may be acceptable in the study (e.g. hypertension);

 10. Significant abnormalities in screening lab hematology or serum chemistries;

 11. Use of any medication known to effect the immune system (e.g. systemic
 corticosteroids) within 30 days of vaccination or planned use during active study
 period (excluding inhaled steroids);

 12. Evidence of confirmed infection with HIV, Hepatitis B or Hepatitis C;

 13. Subjects with IgA (Immunoglobulin A) deficiency (serum IgA < 7 mg/dl or limit of
 detection of assay);

 14. Regular use of antacids, antidiarrheal, loperamide, bismuth subsalicylate,
 diphenoxylate or similar medication less than 2 weeks prior to enrolling in the study
 and through the inpatient portion of the study;

 15. Known or suspected alcohol abuse or illicit drug use within the last year, positive
 urine toxicology for drugs of abuse;

 16. Persons who are committed to an institution (by virtue of an order issued either by
 the judicial or the administrative authorities);

 17. Persons who are in a dependent relationship with the sponsor, an investigator or other
 study team members, or the study center.

 18. Any other criteria which, in the investigator's opinion, would compromise the ability
 of the subject to participate in the study, the safety of the study, or the results of
 the study.