Gamma Delta T Cells in AML
Gamma Delta T Cells in AML
The Royal Marsden NHS Foundation Trust is committed to improving patient experience; this
 research is being undertaken to try to develop a novel treatment for patients with Acute
 Myeloid Leukaemia (AML). Researchers aim to develop a new therapy which uses a patient's own
 immune cells called T cells to treat AML. In this study, numbers and properties of T cells
 which can be collected from the blood of patients with AML at various points throughout their
 treatment will be investigated. Blood samples will be collected at the same time as the
 patient's bone marrow test.

 If patients need further bone marrow tests during their course of treatment to assess the
 status of disease, the research team would ask that additional samples are taken at the same
 time as the bone marrow and blood will be collected at the same time as the routine blood
 draw.

 Following collection of blood samples, they will be used to purify a population of blood
 cells called Gamma Delta T cells which have been shown to have a potential role in control of
 cancers. In addition the researchers plan to determine whether it is possible to put a novel
 receptor called a chimeric antigen receptor (CAR) to potentially directly target leukaemia
 cells. Currently this is only an exploratory study and none of the samples collected will be
 used for treatment and is only to assess whether or not this strategy is feasible. This may
 however lead on to studies in the future looking at the safety and effectiveness of this
 strategy. This hopefully will lead in the future to improvements in treatment and outcome for
 patients with AML.

 If patients need further bone marrow tests during their course of treatment to assess the
 status of disease, the research team would ask that additional samples are taken at the same
 time as the bone marrow and blood will be collected at the same time as the routine blood
 draw.

 Following collection of blood samples, they will be used to purify a population of blood
 cells called Gamma Delta T cells which have been shown to have a potential role in control of
 cancers. In addition the researchers plan to determine whether it is possible to put a novel
 receptor called a chimeric antigen receptor (CAR) to potentially directly target leukaemia
 cells. Currently this is only an exploratory study and none of the samples collected will be
 used for treatment and is only to assess whether or not this strategy is feasible. This may
 however lead on to studies in the future looking at the safety and effectiveness of this
 strategy. This hopefully will lead in the future to improvements in treatment and outcome for
 patients with AML.
Inclusion Criteria:

 1. Patients over the age of 18 at time of diagnosis or at time of relapse of disease

 2. Patients with Acute Myeloid Leukaemia (excluding M3) at presentation, remission or
 with refractory or relapsed disease.

 3. Patients must have given informed written consent to participate in this study.

 Exclusion Criteria:

 1. Uncontrolled systemic infection

 2. Currently receiving corticosteroids or other immune-suppressants treatment (except in
 cases where the patient is receiving treatment with replacement doses for adrenal
 insufficiency)

 3. Treatment with bisphosphonates, for instance zoledronate, in the previous 3 months or
 throughout the trial

 4. Active, known or suspected autoimmune disease such as Ulcerative Colitis /
 Inflammatory bowel disease, Addison's disease

 5. Pregnancy or lactation before or during the study

 6. Substance abuse, medical, psychological or social conditions that may interfere with
 the subject's participation in the study

 7. Patients with active Hepatitis B, C or HIV will be excluded from this study