Study of Gene Activity in Fat and Muscle in Diabetics and Healthy Controls
Study of Gene Activity in Fat and Muscle in Diabetics and Healthy Controls
The aim of the present protocol is to study gene activity in fat and muscle tissue in type 2
 diabetics and healthy volunteers after injection of E. coli endotoxin.We hereby hope to gain
 insight in some mechanisms behind the association between inflammation and insulin
 resistance.
Numerous studies have shown an association between low-grade inflammation and
 insulin-resistance in type 2 diabetics. It is also well-known that septic patients often
 develop insulin-resistance. The pathogenetic mechanisms behind the association between
 inflammation and insulin-resistance is not fully understood.

 In this study we create an experimental inflammation by giving E. coli endotoxin 0,3 ng/kg iv
 to type 2 diabetics and healthy controls. Muscle biopsies are taken at 0, 2, 4 and 6 hours
 after injection, while fat biopsies are taken 0, 2, 4, 6 and 8 hours after endotoxin. mRNA
 for adiponectin, leptin, PPAR-gamma, PGC-1, PAI-1 and cytokines in tissue is later measured
 by RT-PCR.

 Blood samples are drawn on an hourly basis until 8 hours after endotoxin injection for common
 biochemical analyses including white blood cell count. Plasma is spared and later analyzed
 for cytokines, PAI-1 and VCAM-1/ICAM-1.
Inclusion Criteria:

 -  Healthy

 -  Type 2 diabetes

 Exclusion Criteria:

 -  Heart failure

 -  Lung disease

 -  Anti-coagulation treatment