PCR Technic Evaluation in the Microbial Diagnostic of Septicemia in Hemodialysis Patients With Catheter.
PCR Technic Evaluation in the Microbial Diagnostic of Septicemia in Hemodialysis Patients With Catheter.
The aims of this study are to evaluate the PCR on whole blood approach to determine rapidly
 both the susceptibility and the identification of Staphylococcus responsible of septicemia in
 patients under hemodialysis with catheter. The results obtained with this novel experimental
 approach will be compared to those obtained on the same clinical samples by the routine
 diagnostic laboratory.
The outlook of current trends indicates that maximum effort is needed to tailor the initial
 antimicrobial therapy in patients under hemodialysis with catheter. Specific emphasis is put
 on early availability of antimicrobial susceptibility results by Diagnostic laboratories to
 optimize the management of those difficult infections.

 Direct whole blood PCR could allow a rapid determination of bacterial susceptibility to
 antibiotics. The study is designed to assess the concordance of a direct whole blood PCR
 targeting staphylococcus and its susceptibility to oracillin with the classical
 microbiological approach of samples from hemodialysis patients with catheter suspected of
 septicemia. This innovative approach should allow a faster diagnostic allowing the clinicians
 to better tailor the initial empirical antimicrobial therapy which has been proven crucial
 for a good clinical management of the patients.

 Moreover, the use of an adequate antimicrobial therapy early in the management of the
 patients, should not only lead to a better clinical outcome but also avoid unnecessary
 prescriptions of drugs which are the main drivers of the emergence and spread of
 antimicrobial resistance.
Inclusion Criteria:

 -  patients over 18 year old,

 -  suspicion of septicemia in hemodialysis patient with catheter,

 -  patient oral consent prior to inclusion in the study.

 Exclusion Criteria:

 -  patients who went through the A&E department,

 -  patients who have received an antimicrobial therapy more than 8 hours long,

 -  patient under legal protection.