Vascular Function and Biomarker Assessments in Healthy Volunteers and in Patients With Type 2 Diabetes Mellitus
Vascular Function and Biomarker Assessments in Healthy Volunteers and in Patients With Type 2 Diabetes Mellitus
The purpose of this study is to evaluate the vascular function and biomarkers in healthy
 volunteers and type 2 diabetic patients.
The present interventional study will be performed in 3 groups of healthy volunteers of
 different ages and in type 2 diabetic patients without an investigational medicinal product
 administrated.
Inclusion Criteria:

 Healthy volunteers:

 -  Age [18 -30], [50-59] and [60-70] years

 -  Male and female healthy volunteers, except with skin types 5 and 6

 -  Body weight ≥ 50 kg and BMI between [18.0 -28.0] kg/m^2 inclusive

 -  Non or ex-smokers (defined as someone who completely stopped smoking for at least 1
 month before the beginning of this study)

 -  No clinically relevant findings in the medical history and physical examination,
 especially with regards to cardiovascular system, lung, liver and renal function

 -  Normal blood and urine laboratory tests

 Patients with type 2 diabetes (T2D) mellitus:

 -  Male and female patients, except with skin types 5 and 6

 -  Age [50 - 70] years

 -  BMI ≤35 kg/m2

 -  T2D patients (according to American Diabetes Association (ADA) criteria i.e. HbA1c >
 6.5% or fasting plasma glucose > 126 mg/dL (7.00 mmol/L) or 2-hour glucose ≥ 200mg/dL
 (11.1mmol/L) after 75g oral glucose or glucose ≥200mg/dL (11.1mmol/L) at any time on
 two separate occasions (historic values and/or at selection)

 -  Currently treated with Standards of Medical Care in T2D at stable doses for at least 3
 months

 -  Antihypertensive drugs allowed except beta-blockers and calcium antagonists

 -  Non or ex-smokers (defined as someone who completely stopped smoking for at least 1
 month before the beginning of this study)

 Non inclusion Criteria:

 Healthy volunteers:

 -  Unlikely to co-operate in the study,

 -  Participation in another interventional study, including last study drug intake, at
 the same time or within the 3 months preceding the selection visit; participation in
 non-interventional registries or epidemiological studies is allowed,

 -  Deprived of his freedom by administrative or court order or under guardianship,

 -  History or evidence of acute or chronic abuse of alcohol consumption of> 21 alcohol
 units per week for males and >14 units per week for females (1 alcohol unit = 100 mL
 of 12% wine; = 30 mL of 40% spirits; = 250 mL of 6% beer),

 -  Positive alcohol breath test,

 -  Positive cotinine test,

 -  Known or suspected to be drug-dependent,

 -  Positive result in urinary screening for drug abuse,

 -  Pregnancy, breastfeeding or lactating,

 -  Regular use of prescribed and non-prescribed drugs in the last 60 days before the day
 of investigations into the study except hormonal contraception (e.g. pill or hormonal
 intrauterine device (IUD) or hormonal implants or Nuva Ring) (if applicable),

 -  Any drug intake of prescribed or non-prescribed drugs including over-counter drugs
 (except acetaminophen), herbal remedies or nutritional supplements such as
 multivitamins in the 2 weeks before the day of investigations unless the investigator
 deems this drug intake to be irrelevant for the purpose of this study and that can be
 temporarily suspended,

 -  Intake of dipyridamol (contraindicated with adenosine infusion)

 -  Known hypersensitivity to adenosine injection,

 -  Any acute or chronic illness or clinically relevant findings (i.e. liver, kidney,
 cardiovascular diseases) in the selection visit examinations,

 Patients with type II diabetes mellitus (T2D):

 -  Unlikely to co-operate in the study,

 -  Participation in another interventional study, including last study drug intake, at
 the same time or within the 3 months preceding the selection visit; participation in
 non-interventional registries or epidemiological studies is allowed,

 -  Deprived of his freedom by administrative or court order or under guardianship,

 -  Unwilling to allow his or her primary care practitioner to be notified of
 participation in the study and for information on a participant's medical history to
 be obtained from the general practitioner,

 -  History or evidence of acute or chronic abuse of alcohol consumption of> 21 alcohol
 units per week for males and >14 units per week for females (1 alcohol unit = 100 mL
 of 12% wine; = 30 mL of 40% spirits; = 250 mL of 6% beer),

 -  Positive alcohol breath test,

 -  Positive cotinine test,

 -  Known or suspected to be drug-dependent,

 -  Positive result in urinary screening for drug abuse,

 -  Pregnancy, breastfeeding or lactating,

 -  Intake of dipyridamol (contraindicated with adenosine infusion)

 -  Known hypersensitivity to adenosine injection,

 -  Any acute or chronic illness or clinically relevant findings (i.e. liver,kidney,
 cardiovascular diseases) in the selection visit examinations.