The Evaluation of the Diagnostic Properties of Intra-operative In-situ Thyroglobin Levels of Cervical Lymph Nodes, in the Discrimination Between Benign and Malignant Lymph Nodes in Thyroid Cancer: a Prospective Multicentre Study.
The Evaluation of the Diagnostic Properties of Intra-operative In-situ Thyroglobin Levels of Cervical Lymph Nodes, in the Discrimination Between Benign and Malignant Lymph Nodes in Thyroid Cancer: a Prospective Multicentre Study.
Cytological examination of punctured lymph nodes is the gold standard for confirming
 metastatic lymph node spread of differentiated thyroid cancers. In order to increase the
 diagnostic sensitivity of fine-needle cyto-punctured lymph nodes, an assessment of Tg levels
 of the aspirate could be included. Although this technique has been well proven, many
 uncertainties remain, especially with regards to a pathological cut-off value and its
 clinical utility when the thyroid is still intact. This uncertainty is mainly due to
 discordancy between low Tg levels found in cytopunctured lymph nodes with normal cytology,
 and their final histopathological analyses.

 To eliminate this uncertainty, cyto-punction will be performed intra-operatively after
 localizing and isolating the target lymph nodes for assessment of cytology and Tg values. The
 thyroid gland might be present or absent (already operated) depending on the case. Finally,
 the cyto-punctured lymph nodes will be excised for complete histopathological analysis. In
 order to determine whether the Tg values are appropriate in cases where the thyroid is
 intact, a control group has been included (First operation for thyroid cancer or benign
 pathology). To eliminate the possible iatrogenic risks of lymph node dissection and resection
 in patients for whom it is not indicated, only lymph nodes found along the incision path for
 neuromonitoring of the recurrent laryngeal nerve (performed systematically) will be analysed
 and excised.
Inclusion Criteria:

 -  Subject aged 18 years and above, male or female, with a valid social security
 coverage;

 -  Subject willing to participate in the study with a signed informed consent;

 -  Subject being operated for a papillary thyroid cancer, re-operated for
 persistent/relapsed lymphadenopathy, or for benign thyroid pathology (Graves disease,
 multinodular goitre, toxic nodule);

 -  Subject having had a blood test for plasma Tg levels and circulating anti-Tg
 antibodies in the week preceding the surgical intervention.

 Exclusion Criteria:

 - Subjects presenting with thyroid cancers of non follicular origin