Topical Chemoprevention of Skin Cancer Biomarkers
Topical Chemoprevention of Skin Cancer Biomarkers
This research study will test how well one topical medications work to prevent the
 development of non-melanoma skin cancers by reversing certain biomarkers in the skin. This
 study is also looking at the optimal dose of a medication in a small number of people.
 Biomarkers are molecules that are found in the body and inside of cells. Some biomarkers are
 associated with specific diseases such as skin cancer. In this study, one topical medication
 will be evaluated; diclofenac. Diclofenac and is approved by the Food and Drug Administration
 (FDA) for other uses. 24 patients will be enrolled in this study by University of Alabama at
 Birmingham.
Men and women (≥ 18 yo)who have been seen as patients in the Dermatology Clinic at the
 University of Alabama at Birmingham with a history of basal cell or squamous cell carcinoma
 of the skin and at least 8 actinic keratoses on the upper extremities are potentially
 eligible for study participation.

 We propose to examine one topical medication which is already FDA approved,with a placebo
 comparator. The purpose of the study is to see if diclofenac applied daily will affect levels
 of specific biomarkers in the skin that are associated with risk of developing skin cancer.
 We hope to see these levels decrease with once daily use of this medication. Results from
 this study will help guide us in a second study where we will look at longer term use of
 these medications and how they are associated with changes in skin biomarkers that are
 related to skin cancer. The second longer study will use the dose (once or twice daily
 topical application of diclofenac and DMFO) that resulted in a decrease in biomarkers, as
 discovered in this currently proposed study.
Inclusion Criteria:

 o Ability to understand and willingness to sign a written informed consent document

 -  ECOG performance status 0-1

 -  Willing and able to participate for the full duration of the study

 -  Greater than 4 weeks from:

 Prior major surgery for any indication Prior chemotherapy, hormonal therapy or radiation
 therapy for cancer o Willing to abstain from: The application of topical medications
 including prescription and over the counter preparations (e.g. Topical preparations
 containing corticosteroids or vitamin A derivatives) to areas of actinic damage for the
 duration of the study. Use of moisturizers/emollients and sunscreens on these areas is
 allowed.

 Chronic (defined as > 3 times/week for more than 2 consecutive weeks/year) NSAID and COX-2
 inhibitor use (other than cardioprotective doses of aspirin < 100 mg po QD) for the
 duration of the study. For routine analgesia, subjects may take acetaminophen as necessary.

 -  Normal organ and marrow function defined as laboratory values falling within the
 specified ranges for the following tests (performed within 14 days of registration)
 Hematologic • WBC > 3,000/ul • Hemoglobin > lower limit of normal • Platelet count >
 100,000/ul Hepatic

 • Total bilirubin < 1.5 X ULN

 • AST (SGOT) < 1.5 X ULN

 -  ALT (SPGT) < 1.5 X ULN Renal

 -  Serum creatinine < 1.5 X ULN

 -  BUN < 1.5 X ULN

 -  Females of childbearing potential must:

 Have been using adequate contraception (abstinence, IUD, birth control pills or spermicidal
 gel with diaphragm or condom) since their last menses Have a documented negative serum
 pregnancy test within 14 days prior to the first dose of study medication Females are not
 considered to be of childbearing potential if they are at least 1 year post-menopausal or
 have had a tubal ligation, bilateral oophorectomy or hysterectomy.

 o The effects of topical DFMO + topical diclofenac on the developing fetus are unknown.
 Therefore all females of childbearing potential must agree to use adequate contraception
 (abstinence, IUD, birth control pills, or spermicidal gel with diaphragm or condom) for the
 duration of study participation.

 Exclusion Criteria:

 o Within 6 months prior to randomization: Use of oral or intravenous corticosteroids for
 more than 2 consecutive weeks Use of inhaled corticosteroids for more than 4 consecutive
 weeks

 o Any of the following in the 4 weeks (or as indicated) prior to randomization: Major
 surgery for any indication Cytotoxic chemotherapy for any indication (including
 methotrexate for arthritis) Anti-cancer treatment of any type other than for a stage 0-2
 non-melanoma skin cancer Hormonal therapy for cancer prevention (including tamoxifen) Note:
 treatment with finasteride/dutasteride for BPH does not render a participant ineligible.

 Radiation therapy Topical medications for the treatment of actinic keratosis or skin cancer
 (retin A, 5-FU, imiquimod) in the 6 months prior to randomization.

 Laser resurfacing, dermabrasion, cryotherapy, chemical peel and electrodissection ±
 curettage in the 6 months prior to randomization.

 Nasally inhaled corticosteroids (except mometasone - Nasonex) Aspirin (>100 mg/day) - Note:
 cardioprotective doses (< 100mg/day) are acceptable.

 NSAIDs (other than aspirin < 100mg/day) or COX-2 inhibitors > 3 times/week for more than a
 two week period Topical steroids

 o Any personal history of: Invasive cancer diagnosed or treated within the past 5 years.
 Participants who have been in remission for 5 years or more and have not required treatment
 in the past 5 years may be eligible if the principal investigator believes there is little
 to no risk of recurrence.

 Solid organ or bone marrow transplant Keloid formation Photosensitivity disorder
 Hypersensitivity or adverse reactions to nonsteroidal anti-inflammatory agents or to DFMO
 Any disease that predisposes to NMSC An immunodeficiency disorder or the use of an
 immunosuppressive drug Any skin disease that would interfere with interpretation of results

 -  Any family history of Ornithine diaminotransferase deficiency in a first degree
 relative

 -  Concurrent use of the following medications or treatments Anticoagulants including
 warfarin and heparin Other NSAIDs (other than aspirin <100 mg/day) on a daily basis
 Topical chemotherapy, cryotherapy, radiotherapy or any other skin lesion treatment to
 areas of skin being followed in this study Systemic therapy with psoralens,
 immunotherapy, retinoids, or radiation therapy Cytotoxic chemotherapy for any reason
 (including methotrexate for arthritis) Laser resurfacing, dermabrasion or chemical
 peels Topical or systemic immunosuppressive therapy.

 -  Females who are pregnant or lactating. Should a woman become pregnant or suspect she
 is pregnant while she is participating in this study she should notify the study
 physician immediately.

 -  Uncontrolled concurrent illness including ongoing or active infection, psychiatric
 illness/social situations that would limit compliance with study requirements or other
 underlying serious medical condition which, in the investigator's opinion, might
 preclude study participation.