Establishment of a Cohort of Patients With Sepsis Encephalopathy
Establishment of a Cohort of Patients With Sepsis Encephalopathy
In this study, the investigators aimed to initially explore and describe the expression
 profiles, pathological processes, and molecular mechanisms of cytokine differential
 indicators such as NLRP3, NLRP1, and AIM2 in patients with SAE by establishing a cohort of
 SAE patients; and anchoring by enrichment analysis Risk cytokines and pathways related to the
 occurrence of SAE patients; provide theoretical guidance and basis for in-depth study of SAE
 occurrence mechanism and screening of early warning indicators and treatment targets.
According to the inclusion and exclusion criteria of this study, relevant patients who
 attended the emergency department of our hospital were collected, and the SAE cohort was
 established. participants were divided into SAE group and non-SAE group based on whether SAE
 occurred after discharge and one year follow-up. Another 20 patients with non-infected SIRS
 were selected as the control group.For the above three groups of patients, at the same time
 as the biochemical, routine, and culture examinations of lumbar puncture and cerebrospinal
 fluid, 5 ml of cerebrospinal fluid was retained for cytokine detection such as NLRP1, NLRP3,
 AIM2, and NURRI.All were given active anti-shock, and actively sought for infections through
 traditional imaging and quantum dot spectroscopy techniques for targeted anti-infective
 therapy, maintenance of circulation and stable internal environment.Research main
 indicators:Patient baseline data, GCS, CAM-ICU, RASS, APACHE-II, SOFA, IL, IL6, CPR, PCT,
 WBC, N%, liver and kidney function indicators, NLRP1, NLRP3, AIM2 and other indicators,
 follow-up of patient survival rate.
Inclusion criteria:

 1. sepsis 3.0 diagnostic criteria;

 2. Estimated length of hospital stay> 24h;

 3. ages 18-89;

 4. Acute brain dysfunction: delirium, coma, epilepsy, focal neurological deficit;

 5. Patients who meet the non-infectious SIRS diagnostic criteria

 Exclusion criteria:

 1. Diagnosis of patients with brain injury before admission, including Alzheimer's
 disease, craniocerebral injury, etc .;

 2. Primary brain injury (cerebral hemorrhage, cerebral infarction, etc.), secondary brain
 injury (liver brain, lung brain, uremia encephalopathy, pancreatic encephalopathy,
 metabolic encephalopathy, Wake encephalopathy, etc.);

 3. pregnant and lactating women;

 4. Those who have undergone bypass surgery in the past 3 months;

 5. Hearing and vision impairment;

 6. mental illness and melanoma;

 7. Abnormal coagulation, active bleeding

 8. Patients with infection at lumbar puncture site

 9. Meningeal leukemia patients

 10. Patients with other types of encephalopathy