EffectS of Non-nutritive sWeetened Beverages on appetITe During aCtive weigHt Loss
EffectS of Non-nutritive sWeetened Beverages on appetITe During aCtive weigHt Loss
The effects of non-nutritive sweetened (NNS) beverages on weight management and psychological
 indices of appetitive behaviour have not been assessed in both weight loss and maintained
 weight loss phases over the long term to determine whether NNS beverages may assist in
 efforts to lose weight and maintain weight loss and how this affects appetite expression
 (satiety, food choice, craving, ease of dieting etc.). Thus the present research will
 determine the impact of NNS beverages as compared to water on weight loss and weight loss
 maintenance, satiation, satiety, cravings for sweet, exercise efforts and mood state across
 both a period of weight-loss as well as a weight-maintenance phase. To determine if the
 effects of NNS on appetite are beneficial or detrimental to successful weight management, the
 study will also systematically compare the effects of NNS beverages, water and caloric
 beverages (CBs) on appetite (hunger, satiety, liking and wanting) and energy intake
 (including food choice and caloric compensation) across the whole day during active weight
 management in a subset of participants.
The study will use a parallel randomized design with two arms; participants will either be
 randomized to a non-nutritive sweetener (NNS) beverage condition (active) or a water
 condition (control). A total of 432 overweight and obese participants (Body Mass Index; BMI
 range: 27-35 kg/m2) will be enrolled in the study. A 3-month weight loss diet phase will
 initially be completed, with participants receiving nutritional advice at weekly group
 sessions whilst additionally incorporating the active or control products into their diet
 (weeks 0-12). This programme is based on a model successfully used in an American sample
 (Peters et al., 2014) which will be adapted for a UK sample. This will be followed by a
 9-month weight maintenance phase (weeks 13-49) in which participants will be advised to
 follow a general healthy diet in accordance with dietary guidelines (continuing to
 incorporate the active or control beverages) but will only attend monthly group sessions.
 This will be followed by a one year post assisted weight maintenance period in which
 participants will continue to consume the active or control beverages every day and will be
 asked to complete questionnaire measures, cognitive tasks, biomarkers of health (blood
 samples, if willing) and body composition data (weight, sagittal diameter and hip and waist
 circumference measures; DXA (for Subset 2)) at the end of this period. A subset of
 participants (Subset 1) will additionally undergo appetite probe days (n = 116), attending
 the Study Centre at 15 points throughout the trial (3 sessions at baseline, 3 sessions at the
 start of the weight loss programme, 3 sessions at the end of the weight loss programme, 3
 sessions at the end of assisted weight maintenance and 3 sessions at the end of the one year
 post assisted weight maintenance period). A separate subset (Subset 2) will complete
 biological assessment measures (n =50) involving body composition (DXA) scans at 4 points
 throughout the trial (at baseline, end of weight loss, end of assisted weight maintenance,
 and at a year post assisted weight maintenance visit) and all participants will be asked to
 provide blood samples at these four points but will not be excluded from the trial should
 they not wish to.

 Both groups will be asked to ensure that they consume at least two servings (330 ml) of their
 elected beverages dependent on condition (NNS vs. water). All beverages will be picked by
 participants (from a permitted range) and then delivered to them in bi-weekly batches using
 an online delivery-style system. Primary study visits for body weight, waist circumference,
 hip circumference, sagittal diameter, and blood pressure assessments will be carried out at
 various stages throughout the trial. Initial assessments will be at baseline (week -2),
 monthly over the weight loss period (weeks 4, 8 and 12), monthly over the assisted weight
 maintenance period (weeks 16, 20, 24, 28, 32, 36, 40, 44 and 48) and at a 1 year post
 assisted weight maintenance visit with Subset 2 additionally providing body composition (DXA)
 scans at baseline, at the end of the weight loss period, at the end of the assisted weight
 maintenance period and at a 1 year post assisted weight maintenance visit. During the weight
 loss phase, participants will meet in groups (10-12 participants) with a dietician-trained
 researcher every week (12 visits) followed by monthly meetings during the assisted weight
 maintenance phase (9 visits). Participants will additionally be asked to complete a series of
 questionnaires throughout the intervention period and complete a series of diet diaries
 (3-day diet diaries and food frequency questionnaires; FFQs) to assess compliance throughout
 the trial. Furthermore, empty product packaging (labels or bottles) will be collected as an
 additional compliance measure. The probe days to be completed by Subset 1 will involve
 participants attending the Study Centre at various times over an 8-hour day period for 3
 meals (breakfast, lunch and dinner) and 1 drink (NNS, sugar sweetened beverage (SSB) or
 water) for a total of 15 sessions. Adverse events and concomitant medicine will be registered
 continuously throughout the study in relevant forms. For each participant, the total study
 duration is 100 weeks (not including recruitment and screening) and includes a total of 39
 visits (with additional visits for Subsets 1 and 2).

 12-week weight loss programme. The 12-week Weight Loss Programme (WLP) will be based on the
 current successful UK-based dietician-designed nutritional knowledge and group work programme
 used within the Kissileff Laboratory, the content of which will be enhanced by a social
 cognition based programme currently used in Colorado. This employs a group based approach
 (10-12 participants) with frequent meetings (one per week) for a one hour session with a
 dietician trained researcher who will supervise the sessions and who will provide materials
 and 'home work' for the participants to complete before their next session. This programme
 will be tailored for the UK and specific behavioural components such as advice on
 self-monitoring, stimulus control, stress management, slow eating rate and related behaviours
 will be covered. An exercise component will also be included in which exercise activities are
 noted in a log and an additional online daily drinking log will also be included. The
 exercise log will be completed weekly and brought to each session as a means of monitoring.
 Weight will be measured at the start and end of this phase and weekly during the WLP as part
 of group sessions (at the end of the group session) and will be conducted privately with each
 participant in a separate room. Participants will also be asked to complete food frequency
 questionnaires (FFQs) once per month and online 3-day diet diaries before beginning the WLP
 and at the end of the WLP and will be given instructions on how to do so. In week 12 a
 further visit (independent from group sessions) will be scheduled individually with each
 participant to complete additional questionnaire and cognitive measures (Visit 13).

 36-week assisted weight maintenance phase. Participants will be asked to attend one hour
 monthly group sessions with a dietician-trained researcher (10-12 participants per group)
 during the 36-week assisted weight maintenance phase running from week 13-48. These meetings
 will provide an opportunity for participants to discuss any concerns and to provide further
 reminders of nutritional advice. Body weight will be measured at the end of each group
 session and will be conducted privately with each participant in a separate room.
 Participants will also be asked to complete a series of questionnaires measuring appetite
 control and ease of dieting and measures of dietary compliance alongside providing the online
 daily beverage log and weekly paper exercise log. Participants will also be asked to complete
 FFQs monthly during this period. At the end of this phase further visits (independent from
 group sessions) will be scheduled to complete the final measures of appetite and food choice
 and to provide their final online 3-day diet diary.

 Fasting blood samples will be analysed for glucose, insulin, lipids, triglycerides, high
 density lipoprotein (HDL), low density lipoprotein (LDL), total cholesterol profiles and
 Hba1.

 One year post assisted weight maintenance and visit After the final study visit
 post-maintenance (week 48), participants will continue to be provided either NNS or water
 beverages for a 12-month duration. They will be asked to return their empty beverage
 packaging monthly during this phase (weeks 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96)
 and will complete retrospective appetite questionnaires alongside additional questionnaires
 upon returning their packaging. They will also complete daily online beverage logs online
 over the duration of the 12-month period and will complete a final online 3-day food diary
 which they present at Visit 39 (week 100). Participants in Subset 1 will additionally undergo
 a final set of probe days in weeks 98-99 (Visits 38a, 38b, and 38c). Subset 2 will also
 provide their final DXA scan at the one year post assisted weight maintenance period visit
 (Visit 39). At this final visit participants will be asked to provide a fasting blood sample
 as well as complete a range of computer tasks, questionnaires and food diaries. Their body
 weight will be measured and their waist, hip and sagittal diameter will be taken in a
 separate room along with their blood pressure

 Subsets Subset 1 - Appetite Probe Days A voluntary subset of randomly selected participants
 (n=116) will be invited to participate in the appetite probe day sessions to assess changes
 in appetite response to caloric and non-caloric beverages (water, NNS and caloric beverages;
 CB). These probe days will be measured at baseline (Visits 2a, 2b & 2c; weeks -2 to 0), at
 the start of the weight loss programme (Visits 3a, 3b and 3c; weeks 1 to 2), at the end of
 the weight loss period (Visits 13a, 13b and 13c; weeks 11 to 12) at the end of the assisted
 weight maintenance period (Visits 24a, 24b, 24c; weeks 47 to 48) and at the end of the 1 year
 post assisted weight maintenance period (Visits 38a, 38b, & 38c). These sessions will be
 scheduled a minimum of 3 days after one another (minimum completion of 2 weeks) and must all
 be completed within a maximum of 3 weeks. At each point one probe day will examine the
 response to a caloric beverage (ie a sugar sweetened beverage; SSB) and then the second and
 third the response to NNS and water (the order of all three drinks will be counterbalanced
 across participants and probe days). These will be provided after breakfast and 1 hour before
 lunch (approximately 12pm). Compensation will then be tracked at an ad libitum test lunch and
 an evening meal. Participants will be blinded to the study condition (no information will be
 provided about the drinks during the probe days) however the sensory characteristics of the
 beverages may be evident. Cognitive and sensory cues cannot be matched but are important
 determinants of real world appetite responses.

 The gold standard 8-hour design will be used. The appetite probe day will be conducted at the
 Kissileff Laboratory study centre and will involve participants attending the centre for a
 fixed breakfast (breakfast is calibrated based on weight bands which will be determined at
 the Screening session; prior to the end of WLP and prior to the end of the assisted weight
 maintenance phase to ensure accurate weight banding), the study beverage, ad libitum lunch
 and ad libitum dinner as well as a take-home snack pack for the remainder of the evening.
 Participants will complete appetite VAS pre- and post- each meal as well as completing these
 ratings at hourly intervals throughout the test day. They will be free to leave the Study
 Centre after consuming their meals and will be provided with water as well as a specified
 time to return for their next meal.

 Subset 2 - DXA Scans In addition to trial participation, Subset 2 (n = 50) be asked to
 provide fasting full body DXA scan at dedicated visits 1d, 14a, 25a, and 39 (weeks 0, 12, 48
 and the one year post assisted weight maintenance visit respectively). The DXA scan will be
 used to assess changes in body composition during the trial.

 Participants will be reminded during the Screening session that they do not have to take part
 in the additional testing (Subsets 1 or 2) or provide blood samples to be eligible for
 enrolment in the trial.
Inclusion Criteria:

 -  BMI ≥ 27.0 and ≤ 35.0 kg/m2

 -  Healthy

 -  Age between 18 and 65 years (inclusive)

 -  Regular consumer of cold NNS or water beverages (>3 per week but < 2 litres per day)

 -  Acceptance of randomization to either a water or NNS beverage condition

 -  Willing to abstain from consuming NNS beverages (if randomized to the water condition)
 and SSBs for the duration of the trial.

 -  Provided voluntary written informed consent

 -  Not planning on moving outside of the local area in the next 24 months

 -  Two individuals from the same household complying with the inclusion and exclusion
 criteria will be permitted to participate in the trial however, in such instances, the
 individuals will be randomized to the same group

 Exclusion Criteria:

 -  Not willing to discontinue drinking NNS beverages for the duration of the trial should
 they be assigned to the water group and vice versa;

 -  Not willing to consume NNS beverages or water for the duration of the trial;

 -  Expressing a significant dislike of NNS beverages (at least moderately likeable on a 5
 point likert scale);

 -  Excess consumer of chilled beverages or NNS beverages (more than 2 litres a day);

 -  A BMI below 27 or above 35 kg/m2;

 -  Below the age of 18 or above 65 years;

 -  A first year University Student (to avoid the 'freshman 15' phenomenon of weight gain
 within the first year of attending University);

 -  Significant health problems (including a history of cardiovascular diseases);

 -  Gastro-intestinal problems or diabetes;

 -  Taking any medication or supplements known to affect appetite or weight within the
 past month and/or during the study;

 -  Stated major psychiatric disorder (made clear by medicines specified);

 -  Current depression (made clear by medicines specified);

 -  Currently being treated for a psychological condition;

 -  Being pregnant, planning to become pregnant, breastfeeding or less than 6 months
 post-partum;

 -  Planning on moving away from the local area within the next two years;

 -  History of anaphylaxis to food; known food allergies or food intolerance;

 -  Dislike of > 25% of the study foods offered on the appetite probe day (Subset 1 only);

 -  Non breakfast eaters (subset 1 only);

 -  Smoking or having ceased smoking in the last six months;

 -  Currently dieting, having ceased a diet in less than 4 weeks, or having lost
 significant amounts of weight in the previous year;

 -  Engaging in regular intense exercise;

 -  Having significantly changed their physical activity patterns in the past 4 weeks or
 intending to change them during the study (other than to adhered to advice given in
 the behavioural intervention);

 -  Inability to adhere to the programme such as inability to increase activity level;

 -  Receiving systemic or local treatment likely to interfere with evaluation of the study
 parameters;

 -  Working in appetite or feeding-related disciplines;

 -  Being on specific food avoidance diets;

 -  Having had bariatric surgery;

 -  Having abnormal eating behaviour (restrained eaters measured by the Dutch Eating
 Behaviour Questionnaire - Restraint Scale [DEBQ-R] with a cut-off point of more than 4
 (van Strien et al,1986); and binge eaters measured by the Binge Eating Scale with a
 cut-off point of 27 or over (Gormally et al, 1982))