Rapid and Accurate Detection of Major Mycobacterium Species in Pulmonary Infection Based on GenSeizer Platform
Rapid and Accurate Detection of Major Mycobacterium Species in Pulmonary Infection Based on GenSeizer Platform
Comparison of rapid detection methods of mycobacterial pathogens, GenSeizer v.s. PCR reverse
 hybridization.
The investigators developed a new platform called GenSeizer, which combines bioinformatics
 analysis of a large dataset with multiplex PCR-based targeted gene sequencing, to identify
 eleven major Mycobacterium species implicated in human disease. Simultaneous detection of
 certain antibiotic resistance genotypes, i.e., erm(41) and rrl in M. abscessus, is also
 feasible. This platform detected both reference strains and clinical isolates with a high
 degree of specificity and sensitivity.

 In this study, the investigators plan to verify the detection efficiency of GenSeizer in
 clinical samples (sputum, alveolar lavage fluid) and compare it with existing methods on the
 market (PCR reverse hybridization) and gold standard method (target sequencing after
 incubation)
Inclusion Criteria:

 -  Suspected cases of mycobacterial lung infection

 Exclusion Criteria:

 -  No sputum

 -  Refused or unable to tolerate bronchoscopy