A Phase 1 Study of the EZH2 Inhibitor Tazemetostat in Pediatric Subjects With Relapsed or Refractory INI1-Negative Tumors or Synovial Sarcoma
A Phase 1 Study of the EZH2 Inhibitor Tazemetostat in Pediatric Subjects With Relapsed or Refractory INI1-Negative Tumors or Synovial Sarcoma
This is a Phase I, open-label, dose escalation and dose expansion study with BID (suspension)
 and TID (tablet) oral dose of tazemetostat. Subjects will be screened for eligibility within
 14 days of the planned first dose of tazemetostat. A treatment cycle will be 28 days.
 Response assessment will be evaluated after 8 weeks of treatment and subsequently every 8
 weeks while on study.

 The study has two parts: Dose Escalation and Dose Expansion.

 Dose escalation for subjects with the following relapsed/refractory malignancies:

 -  Rhabdoid tumors:

 -  Atypical teratoid rhabdoid tumor (ATRT)

 -  Malignant rhabdoid tumor (MRT)

 -  Rhabdoid tumor of kidney (RTK)

 -  Selected tumors with rhabdoid features

 -  INI1-negative tumors:

 -  Epithelioid sarcoma

 -  Epithelioid malignant peripheral nerve sheath tumor

 -  Extraskeletal myxoid chondrosarcoma

 -  Myoepithelial carcinoma

 -  Renal medullary carcinoma

 -  Other INI1-negative malignant tumors (e.g., dedifferentiated chordoma) (with Sponsor
 approval)

 -  Synovial Sarcoma with a SS18-SSX rearrangement Dose Escalation cohorts are closed to
 enrollment.

 Dose Expansion at the MTD or the RP2D

 -  Cohort 1 - ATRT (closed to enrollment)

 -  Cohort 2 - MRT/RTK/selected tumors with rhabdoid features (closed to enrollment)

 -  Cohort 3 - INI-negative tumors:

 -  Epithelioid sarcoma

 -  Epithelioid malignant peripheral nerve sheath tumor

 -  Extraskeletal myxoid chondrosarcoma

 -  Myoepithelial carcinoma

 -  Renal medullary carcinoma

 -  Chordoma (poorly differentiated or de-differentiated)

 -  Other INI1-negative malignant tumors (e.g., dedifferentiated chordoma) with Sponsor
 approval

 -  Cohort 4 - Tumor types eligible for Cohorts 1 through 3 or synovial sarcoma with
 SS18-SSX rearrangement (closed to enrollment)
Inclusion Criteria:

 1. Age (at the time of consent/assent): ≥6 months to <18 years

 - Cohort 4 only: ≥10 years to <18 years

 2. Performance Status:

 -  If <12 years of age: Lanksy Performance Status >50%

 -  If ≥12 years of age: Karnofsky Performance Status >50% NOTE: If subject is unable
 to walk due to paralysis, but is mobile in a wheelchair, subject is considered to
 be ambulatory for the purpose of assessing their performance status.

 3. Has provided signed written informed consent/assent

 4. Has a life expectancy of >3 months

 5. Has relapsed or refractory disease and no standard treatment options as determined by
 locally or regionally available standards of care and treating physician's discretion

 6. Is ineligible or inappropriate for other treatment regimens known to have effective
 potential

 7. Has a documented local diagnostic pathology of original biopsy confirmed by a Clinical
 Laboratory Improvement Amendments (CLIA)/College of American Pathologists (CAP) or
 equivalent laboratory certification

 8. Has all prior treatment (i.e., chemotherapy, immunotherapy, radiotherapy) related
 clinically significant toxicities resolve to ≤ Grade 1 per CTCAE, version 4.03 or are
 clinically stable and not clinically significant, at time of enrollment

 9. Has completed a prior therapy (ies) according to the criteria below:

 -  Other investigational study agent (any medicinal product that is not approved in
 the country of treatment for any indication, adult or pediatric) (At least 30
 days or five half-lives, whichever is longer, since last dose prior to the first
 dose of tazemetostat)

 -  Chemotherapy: cytotoxic (At least 14 days since last dose of chemotherapy prior
 to first dose of tazemetostat)

 -  Chemotherapy: nitrosoureas (At least 6 weeks since last dose of nitrosoureas
 prior to first dose of tazemetostat)

 -  Chemotherapy: non-cytotoxic (e.g., small molecule inhibitor) (At least 14 days
 since last dose of non-cytotoxic chemotherapy prior to first dose of
 tazemetostat)

 -  Monoclonal antibody (ies) (At least 28 days since the last dose of any monoclonal
 antibody prior to first dose of tazemetostat)

 -  Immunotherapy (e.g., tumor vaccine) At least 6 weeks since last dose of
 immunotherapy agent(s) prior to first dose of tazemetostat)

 -  Radiotherapy (RT) (At least 14 days from last local site RT prior to first dose
 of tazemetostat/At least 21 days from stereotactic radiosurgery prior to first
 dose of tazemetostat/At least 12 weeks from craniospinal, ≥ 50% radiation of
 pelvis, or total body irradiation prior to first dose of tazemetostat)

 -  Hematopoietic growth factor (At least 14 days from last dose of hematopoietic
 growth factor prior to first dose of tazemetostat)

 -  Hematopoietic cell transplantation (At least 60 days from infusion of
 hematopoietic cells prior to first dose of tazemetostat)

 10. Has adequate hematologic (bone marrow and coagulation factors), renal and hepatic
 function as defined by criteria below:

 -  Hematologic (BM Function):

 -  Hemoglobin ≥ 8 g/dL

 -  Platelets ≥100,000/mm^3 (≥100 x 10^9/L)

 -  ANC ≥1,000/mm^3 (≥1.0 x 10^9/L)

 -  Hematologic (Coagulation Factors):

 -  INR/ PTd ≤1.5 ULN

 -  PTT ≤1.5 ULN

 -  Fibrinogen ≥0.75 LLN

 -  Renal Function (creatinine clearance or serum creatinine):

 -  Calculated creatinine clearance ≥50 mL/min/1.73m^2

 -  Serum creatinine 6 months to 1 year: male 0.6 mg/dL (53 µmol/L) female 0.5
 mg/dL (44 µmol/L)

 -  Serum creatinine 1 to < 2 years: male 0.6 mg/dL (53 µmol/L) female 0.6 mg/dL
 (53 µmol/L)

 -  Serum creatinine 2 to < 6 years: male 0.8 mg/dL (71 µmol/L) female 0.8 mg/dL
 (71 µmol/L)

 -  Serum creatinine 6 to <10 years: male 1 mg/dL (88 µmol/L) female 1 mg/dL (88
 µmol/L)

 -  Serum creatinine 10 to <13 years: male 1.2 mg/dL (106 µmol/L) female 1.2
 mg/dL (106 µmol/L)

 -  Serum creatinine 13 to <16 years: male 1.5 mg/dL (133 µmol/L) female 1.4
 mg/dL (125 µmol/L)

 -  Serum creatinine ≥16 years: male 1.7 mg/dL (150 µmol/L) female 1.4 mg/dL
 (125 µmol/L)

 -  Hepatic Function:

 -  Total bilirubin <1.5 x ULN

 -  ALT or AST <3 x ULN Eligibility can be determined by either total or
 conjugated bilirubin

 11. For subjects with CNS involvement: Subjects must have deficits that are stable for a
 minimum of 14 days prior to enrollment, or seizures that are stable, not increasing in
 frequency or severity and controlled on current anti-seizure medication(s) for a
 minimum of 7 days prior to enrollment

 NOTE: Subjects with leptomeningeal disease or brian tumors with positive cerebral
 spinal fluid cytology are eligible for this study. Subjects may receive
 glucocorticoids (at stable or tapering dose) to control CNS symptoms prior to
 enrollment; however, subjects should receive a stable or tapering dose for at least 7
 days prior to enrollment.

 12. Has a shortening fraction of >27% or an ejection fraction of ≥50% by echocardiogram or
 multi-gated acquisition scan and New York Heart Association Class<2

 13. Has a QT interval corrected by Fridericia's formula (QTcF) ≤450 msec

 14. Is able to swallow and retain orally administered medication and does not have any
 uncontrolled gastrointestinal (GI) condition such as nausea, vomiting, or diarrhea, or
 any clinically significant GI abnormalities that may alter absorption such as
 malabsorption syndromes, hereditary fructose intolerance, glucose-galactose
 malabsorption, sucrose-isomaltase insufficiency, or major resection of stomach and/or
 bowels NOTE: Nasogastric and gastrostomy tube administration of the oral suspension
 formulation of study drug is permitted.

 15. Has sufficient tumor tissue (slides or blocks) available for central confirmatory
 testing of immunohistochemistry and/or cytogenetics/fluorescence in situ hybridization
 (FISH) and/or deoxyribonucleic acid mutation analysis (required for study entry but
 enrollment based on local results)

 16. Is willing and able to comply with all aspects of the protocol as judged by
 Investigator

 17. For female subjects of childbearing potential: Subject must:

 -  Have a negative beta-human chorionic gonadotropin (β-hCG) pregnancy test at time
 of Screening and within 72 hours prior to planned first dose of tazemetostat
 (urine or serum test is acceptable however, positive urine tests must be
 confirmed with serum testing), and

 -  Agree to use effective contraception, as defined in Section 8.6.11 start of
 Screening until 6 months following the last dose of study treatment and have a
 male partner who uses a condom, or

 -  Practice true abstinence (when this is in line with the preferred and usual
 lifestyle of the subject, see Section 8.6.11, or

 -  Have a male partner who is vasectomized with confirmed azoospermia

 18. For male subjects with a female partner of childbearing potential: Subject must:

 -  Be vasectomized or

 -  Agree to use condoms as defined in Section 8.5.11 from first dose of tazemetostat
 until 3 months following the last dose of tazemetostat, or

 -  Have a female partner who is NOT of childbearing potential

 For Dose Escalation Only:

 To be eligible for enrollment in dose escalation, a subject must meet ALL of the following
 criteria in addition to the inclusion criteria listed above for all subjects:

 1. Has evaluable disease as defined as lesions that can be accurately measured at least
 in one dimension by radiographic examination or physical examination and other lesions
 such as bone lesions, leptomeningeal disease, ascites, hepatosplenomegaly from
 disease.

 2. Has one of the following histologically confirmed tumors: (NOTE: Evidence of
 diagnostic pathology of original biopsy confirmed by a CLIA/CAP certified laboratory
 must be available)

 -  Rhabdoid tumor:

 -  ATRT

 -  MRT

 -  RTK

 -  Selected tumors with rhabdoid features

 -  NI1-negative tumor:

 -  Epithelioid sarcoma

 -  Epithelioid malignant peripheral nerve sheath tumor

 -  Extraskeletal myxoid chondrosarcoma

 -  Myoepithelial carcinoma

 -  Renal medullary carcinoma

 -  Other INI1-negative malignant tumors (e.g., dedifferentiated chordoma) with
 Sponsor approval

 -  Synovial sarcoma with SS18-SSX rearrangement (NOTE: Evidence of diagnostic
 pathology of original biopsy confirmed by a CLIA/CAP certified laboratory must be
 available) (Closed to enrollment)

 3. For subjects with ATRT, MRT, RTK, or selected tumors with rhabdoid features only: the
 following test results must be available: Morphology and immunophenotypic panel
 consistent with rhabdoid tumor and Loss of INI1 or SMARCA4 confirmed by IHC, or
 Molecular confirmation of tumor bi-allelic INI1 or SMARCA4 loss/mutation when INI1 or
 SMARCA4 IHC is equivocal or unavailable

 4. For subjects with INI1 negative tumor only:

 the following test results must be available: Morphology and immunophenotypic panel
 consistent with INI1-negative tumors, and Loss of INI1 confirmed by IHC, or Molecular
 confirmation of tumor bi-allelic INI1 loss/mutation when INI1 IHC is equivocal or
 unavailable

 5. For subjects with synovial sarcoma only:

 The following test results must be available:

 Morphology consistent with synovial sarcoma, and Cytogenetics or FISH and/or molecular
 confirmation (e.g., DNA sequencing) of SS18 rearrangement t(X;18)(p11;q11)

 Dose Escalation cohorts are closed to enrollment.

 For Dose Expansion Only:

 Note: To be eligible for enrollment in Dose Expansion, a subject must meet ALL of the
 following criteria in addition to the inclusion criteria for ALL subjects listed above

 1. Has measurable disease

 2. Has one of the following histologically confirmed tumors:

 -  Cohort 1 - ATRT (Closed to enrollment)

 -  Cohort 2 - MRT/RTK/selected tumors with rhabdoid features (Closed to enrollment)

 -  Cohort 3 - INI-negative tumors:

 -  Epithelioid sarcoma

 -  Epithelioid malignant peripheral nerve sheath tumor

 -  Extraskeletal myxoid chondrosarcoma(EMC)

 -  Myoepithelial carcinoma

 -  Renal medullary carcinoma

 -  Chordoma (poorly differentiated or de-differentiated)

 -  Other INI1-negative malignant tumors (e.g., dedifferentiated chordoma) with
 Sponsor approval

 -  Cohort 4 - Tumor types eligible for Cohorts 1 through 3 or synovial sarcoma with
 SS18-SSX rearrangement (Closed to enrollment) NOTE: Evidence of diagnostic
 pathology of original biopsy confirmed by a CLIA/CAP or other Sponsor-approved
 certified laboratory must be available.

 3. For subjects with ATRT/MRT/RTK only - have the following test results available:

 -  Morphology and immunophenotypic panel consistent with rhabdoid tumor, and

 -  Loss of INI1 or SMARCA4 confirmed by IHC, or

 -  Molecular confirmation of tumor bi-allelic INI1 or SMARCA4 loss/mutation when
 INI1 or SMARCA4 IHC is equivocal or unavailable

 4. For subjects with INI1-negative tumors only: The following test results must be
 available:

 -  Morphology and immunophenotypic panel consistent with INI1-negative tumors, and

 -  Loss of INI1 confirmed by IHC, or

 -  Molecular confirmation of tumor bi-allelic INI1 loss/mutation when INI1 IHC is
 equivocal or unavailable

 5. For subjects with synovial sarcoma with SS18-SSX rearrangement (in Cohort 4 ONLY -
 Closed to enrollment): The following test results must be available:

 -  Morphology consistent with synovial sarcoma, and

 -  Cytogenetics or FISH and/or molecular confirmation (e.g., deoxyribonucleic acid
 [DNA] sequencing) of SS18 rearrangement t(X;18)(p11;q11)

 6. For subjects to be enrolled in Cohort 4 (Closed to enrollment): Able to swallow and
 retain orally administered tablets

 Exclusion Criteria:

 1. Has had prior exposure to tazemetostat or other inhibitor(s) of EZH2

 2. Is being actively treated for another concurrent malignancy or is less than five years
 from completion of treatment for another malignancy

 3. Has participated in another interventional clinical study and received investigational
 drug within 30 days or 5 half-lives, whichever is longer, prior to the planned first
 dose of tazemetostat

 4. Has had major surgery within 2 weeks prior to enrollment NOTE: Minor surgery (e.g.,
 minor biopsy of extracranial site, central venous catheter placement, shunt revision)
 is permitted within 2 weeks prior to enrollment.

 5. Has thrombocytopenia, neutropenia, or anemia of Grade ≥3 (per CTCAE 4.03 criteria) or
 any prior history of myeloid malignancies, including myelodysplastic syndrome (MDS).
 Has abnormalities known to be associated with MDS (e.g. del 5q, chr 7 abn) and MPN
 (e.g. JAK2 V617F) observed in cytogenetic testing and DNA sequencing.

 Note: Bone marrow aspirate/biopsy will be conducted following abnormal peripheral
 blood smear morphology assessment conducted by central lab at screening. Cytogenetic
 testing and DNA sequencing will be conducted following an abnormal result of bone
 marrow aspirate/biopsy.

 6. Has a prior history of T-LBL/T-ALL.

 7. Has clinically active heart disease including prolonged corrected QTcF (>450 msec)

 8. Is currently taking any prohibited medication(s) as described in Section 7.3.

 9. Is unwilling to exclude grapefruit juice, Seville oranges and grapefruit from the diet
 and all foods that contain those fruits from time of enrollment to while on study

 10. Has an active infection requiring systemic treatment

 11. Is immunocompromised (i.e. congenital immunodeficiencies), including subjects known
 history of infection with human immunodeficiency virus (HIV)

 12. Has known history of chronic infection with hepatitis B virus (hepatitis B surface
 antigen positive) or hepatitis C virus (detectable HCV RNA)

 13. Has had a symptomatic venous thrombosis within the 14 days prior to study enrollment
 NOTE: Subjects with a history of a deep vein thrombosis 14 days prior to study
 enrollment who are on anticoagulation therapy with low molecular weight heparin are
 eligible for this study

 14. For subjects with CNS involvement (primary tumor or metastatic disease): Have any
 active bleeding, or new intratumoral hemorrhage of more than punctate size on
 Screening MRI obtained within 14 days of starting study drug,or known bleeding
 diathesis or treatment with anti-platelet or anti-thrombotic agents 15.15. Has known
 hypersensitivity to any of the components of tazemetostat or other inhibitor(s) of
 EZH2, or hypersensitivity to Ora-sweet or methylparaben

 16. Has an uncontrolled intercurrent illness including, but not limited to, uncontrolled
 infection, or psychiatric illness/social situations that would limit compliance with study
 requirements 17. For female subjects of childbearing potential: Is pregnant or nursing For
 male subjects: Is unwilling to adhere to contraception criteria from time of enrollment in
 study to at least 30 days after last dose of tazemetostat.