A Study of Circulating Exosome Proteomics In Gallbladder Carcinoma Patients
A Study of Circulating Exosome Proteomics In Gallbladder Carcinoma Patients
This research will be the first study for exosome purified from blood in gallbladder
 carcinoma patients. Proteomics studies will be done in both tumor tissue and the circulating
 exosome from blood specimens. Then, the potential prognostic and predictive biomarkers will
 be searched by bioinformatics to find the correlations between exosome biomarkers and
 gallbladder carcinoma.
Background:

 Exosomes are formed by inward budding of late endosomes, producing multivesicular bodies
 (MVBs), and are released into the environment by fusion of the MVBs with the plasma membrane.
 It has been demonstrated that the content and function of exosomes depends on the originating
 cell and the conditions under which they are produced. Tumor exosome production, transfer and
 education of bone marrow cells supports tumor growth and metastasis.

 Aims of the study:

 Proteomics studies will be done in both tumor tissue and the circulating exosome from the
 gallbladder carcinoma patients. Then, the potential prognostic and predictive biomarkers will
 be searched by bioinformatics to find the correlations between exosome biomarkers and
 gallbladder carcinoma.

 Materials and Methods:

 The investigators will conduct a case-control study in 11 hospitals from China. Cases will be
 the patients with newly-diagnosed gallbladder carcinoma. The controls will be matched （1：1）by
 sex，race and age which will be selected from the patients receiving cholecystectomy due to
 gallstones from the same hospital with the cases. A total of 50 patients with gallbladder
 carcinoma will be recruited. The investigators will obtain the blood and tumor tissue samples
 for further analysis of proteomics studies. Exosome from blood specimens will be isolated and
 purified by sucrose gradient ultracentrifugation. The potential prognostic and predictive
 biomarkers will be searched by bioinformatics to find the correlations between exosome
 biomarkers and gallbladder carcinoma. Data will be collected from January 2018 to December
 2018.
Inclusion Criteria:

 -  Diagnosed with gallbladder carcinoma by imaging tests (cases) Diagnosed with chronic
 cholecystitis by imaging tests (hospital controls)

 Exclusion Criteria:

 -  The pathology diagnosis and imaging does not matched Patients with other malignant
 tumors Patients with other severe systemic diseases