Microbiome and Genetic Analysis of Familial IBD
Microbiome and Genetic Analysis of Familial IBD
Inflammatory bowel disease(IBD) is a chronic inflammatory condition for gastrointestinal
 tract.

 Regarding its pathogenesis, there has been numerous studies to reveal the complex association
 between genetic and environmental factors.

 In Korea, the incidence of IBD is growing rapidly but genetic studies solely including
 patients with Korean descent were not sufficient enough.

 Therefore, the investigators planned to conduct genetic and fecal microbial analysis for the
 60 individuals from 30 Korean IBD families to find out the pathogenesis of IBD.
Under the hypothesis that more risk variants will be observed among the familial IBD patient
 than in IBD patients without any other affected family members, the investigators designed
 genetic and fecal microbiome analysis for 60 patients form 30 families.

 After extracting the DNA from blood samples, whole genome sequencing will be performed and
 data will be comprared with the previously reported variances. Novel variances or incidence
 of specific variances will be measured.

 Genome-wide single nucleotide polymorphism array using Immunochip will be performed to search
 common genetic variants and to calculate genetic risk score of IBD.

 In this study, fecal microbiome is a surrogate marker for the enviromental aspect of
 pathogenesis of IBD. The investigators assumed that family members are sharing similar mode
 of lifestyle therefore we're presumed that their fecal microbial composition is alike.

 Comparing genetic and microbial datas altogether with the data from unaffected family
 member(Healthy internal control), investigators expecting to explain the genetic and
 enviromental aspect of IBD pathogenesis.
Inclusion Criteria:

 -  60 individuals from 30 families of Crohn's disease or ulcerative colitis.

 -  Unaffected 30 individuals from each family as healthy internal control.

 Exclusion Criteria:

 -  Person with history of using antibiotics or probiotics within previous 4 weeks.