Comparison of Pre-procedural PCR and Culture-based UTI Assessment Prior to Intravesical Botox Injection
Comparison of Pre-procedural PCR and Culture-based UTI Assessment Prior to Intravesical Botox Injection
This study is a retrospective record review to determine whether a novel multiplex PCR assay
 can be utilized as a screening method for UTI in patients receiving Botox injection to treat
 overactive bladder (OAB).
The novel multiplex real-time PCR assay with mixed floral antibiotic resistance profiling
 offers a higher degree of sensitivity and specificity than conventional culture methods in
 the identification of UTI pathogens as determined by a previous prospective comparison study.
 This is a retrospective record review comparing PCR and culture-based assessments for UTI in
 patients receiving Botox injection to treat overactive bladder (OAB). Investigators aim to
 determine whether M-PCR/P-AST can be utilized as a screening method for UTI in these patients
 and whether whether antibiotic prophylaxis, peri-procedural antibiotics, and post-injection
 antibiotic therapy based on patients' pre-procedural M-PCR/P-AST results in improved response
 to Botox injection compared to standard of care. The attributes and advantages of PCR testing
 over the use of traditional urine cultures, and the potential identification of polymicrobial
 infections with complicated resistance sharing mechanisms, may significantly improve patient
 care.
Inclusion/Exclusion Criteria:

 -  Subject is male or female

 -  >18 years of age at date of injection

 -  Subject receiving BoNT injection for symptoms of OAB (including urgency, urinary
 incontinence, and detrusor overactivity) refractory to first-line therapies