Analysis of the Results From Influenza Rapid Test A Positive and H1N1 Real Time RT-PCR
Analysis of the Results From Influenza Rapid Test A Positive and H1N1 Real Time RT-PCR
The first outbreak of a new H1N1 influenza pandemic originated in the North America in April
 2009. As of July 1, 2009, a total of 77,201 cases were accumulated in 103 countries around
 the world and the mortality rate of was about 0.43%. Alignment and analysis on gene sequences
 of the new H1N1 influenza virus found that it contains extremely homologous gene composition
 with that of the swine influenza viruses (swine flu) identified in Europe and North America
 in last century. Thus the virus strain was later renamed as a novel influenza H1N1. In
 general, the symptoms caused by the new influenza H1N1 infection was very similar to those
 resulted from seasonal influenza viral infection. Thus, it is difficult to distinguish the
 various influenza strains responsible for the infections only by clinical appearance. To
 compare the accuracy, specificity and sensitivity and speed in identifying the new influenza
 H1N1 in suspected cases, the investigators extracted RNA from influenza A-positive reactive
 specimens identified by a Influenza Rapid Test, for a Real-time PCR method to further detect
 the presence of swine H1 gene. In addition, the titer of H1N1 virus, the color development on
 the test stripe and clinical symptoms in patients were significantly associated. Finally,
 Real-time PCR products were subjected to sequence determination to explore potential new
 influenza pathogenicity, transmissibility and drug usage.
Inclusion Criteria:

 -  Influenza Rapid Test Influenza A(+) B(-)

 -  Influenza Rapid Test Influenza A(+) B(+)

 -  Influenza Rapid Test Influenza A(-) B(-) was negative control

 Exclusion Criteria:

 -  Influenza Rapid Test Influenza A(-) B(+)