Study of Pap Smears From Patients Enrolled on Clinical Trial GOG-171
Study of Pap Smears From Patients Enrolled on Clinical Trial GOG-171
RATIONALE: Studying Pap smears in the laboratory from women with atypical glandular cells of
 unspecified significance may help doctors learn more about changes that may occur in DNA and
 identify biomarkers related to cancer.

 PURPOSE: This research study is looking at Pap smears from women enrolled on clinical trial
 GOG-171.
OBJECTIVES:

 Primary

 -  To determine the diagnostic accuracy of using previously reported morphometric criteria
 for chromatin distribution, shortest distance between nuclei, and/or the ratio of
 nucleoli area to nucleus area in a conventional Pap smear from patients with a cytologic
 diagnosis of atypical glandular cells of unspecified significance (AGUS) to predict the
 presence of significant glandular lesions (i.e., adenocarcinoma in situ [AIS] or
 invasive cancer) and/or squamous lesions (i.e., cervical intraepithelial neoplasia [CIN2
 or CIN3]) of the cervix.

 Secondary

 -  To optimize the diagnostic accuracy of using chromatin distribution, shortest distance
 between nuclei, and/or the ratio of nucleoli area to nucleus area in a conventional Pap
 smear from patients with a cytologic diagnosis of AGUS to predict the presence of
 significant glandular lesions (i.e., AIS or invasive cancer) and/or squamous lesions
 (i.e., CIN2 or CIN3) of the cervix.

 OUTLINE: Previously collected slides from Pap smears of patients enrolled on clinical trial
 GOG-171 are assessed for nuclear chromatin distribution, the shortest distances between the
 central points of neighboring cell nuclei, and nucleoli area/nucleus area (N/N) ratio via
 morphometric analysis.
DISEASE CHARACTERISTICS:

 -  Cytologically confirmed atypical glandular cells of unspecified significance

 -  Original Pap smear used to define the diagnosis must be available for review

 -  Previously enrolled by GOG-JAPAN onto clinical trialGOG-171

 -  Must have been eligible and evaluable for the primary objective of GOG-171

 -  Gave permission to use specimens for future cancer research in GOG-171

 -  Underwent a loop electrosurgical excision procedure/large loop excision of the
 transformation zone (LEEP/LLETZ) procedure on clinical trial GOG-171 to determine the
 presence of cervical intraepithelial neoplasia (CIN-2 or CIN-3), significant glandular
 lesions (AIS), or invasive cancer

 PATIENT CHARACTERISTICS:

 -  Not specified

 PRIOR CONCURRENT THERAPY:

 -  See Disease Characteristics