The Impact of Insulin Therapy on Protein Turnover in Pre-Diabetic Cystic Fibrosis Patients
The Impact of Insulin Therapy on Protein Turnover in Pre-Diabetic Cystic Fibrosis Patients
Insulin replacement therapy may be effective in breaking the cycle of protein catabolism,
 undernutrition and overall clinical deterioration in pre-diabetic, insulin insufficient CF
 youth because of its potent anabolic effect. A significant number of CF patients might
 benefit from this therapeutic approach with a substantial impact on morbidity and mortality.
Insulin insufficiency related to pancreatic fibrosis and β-cell dysfunction is present in
 almost every cystic fibrosis (CF) patient. Progressive abnormalities in insulin secretion
 begin in childhood, and, in adults, CF related diabetes (CFRD) is eventually present in more
 than half of the CF population. CFRD is associated with weight loss, protein catabolism, loss
 of lean body mass (LBM), and early death from lung disease and malnutrition. The negative
 consequences of diabetes are just the "tip of the iceberg", since clinical deterioration has
 been documented to begin in the pre-diabetic period. Non-diabetic glucose tolerance
 abnormalities in CF are associated with protein catabolism, weight loss and lung function
 decline, all of which correlate with the severity of insulin secretory defects, suggesting a
 key pathologic role for insulin insufficiency. Insulin is a potent anabolic hormone, critical
 for maintenance of body weight and muscle mass. In a placebo-controlled clinical trial,
 insulin therapy improved body mass index (BMI) and LBM in patients with very early CFRD (CFRD
 without fasting hyperglycemia), and this is now standard care for these patients. There is
 growing preliminary evidence that insulin therapy is beneficial even earlier, in CF patients
 with pre-diabetes due to insulin insufficiency. Given the universal prevalence of insulin
 insufficiency in CF, the high lifetime risk of developing diabetes, the clinical impact of
 insulin insufficiency on protein catabolism and survival in CF, and the critical importance
 of maintaining body weight and LBM in this population, there is an urgent need to determine
 whether insulin replacement therapy should be instituted for anabolic purposes prior to the
 actual onset of diabetes and, if so, to ascertain the optimal regimen. The current protocol
 describes a double-blind, placebo-controlled trial to determine whether insulin therapy
 improves protein catabolism in youth with CF and abnormal glucose tolerance, and to explore
 differences in efficacy between multiple daily pre-meal insulin dosing (as is currently
 standard for early CFRD) versus a more convenient once daily basal insulin dose (as has been
 used in small uncontrolled pilot studies). The findings of this study will provide a
 mechanistic rationale for instituting insulin in youth with CF and pre-diabetes, and will
 inform both research studies and clinical practice as to the best regimen for insulin
 delivery in this population.
Inclusion Criteria:

 1. Diagnosis of cystic fibrosis, age 10-25 years

 2. A standard routine annual OGTT performed within 12 months of randomization

 3. Abnormal glucose tolerance, with a fasting glucose level <126 mg/dl and

 -  The 1-hr OGTT glucose is ≥200 mg/dl but the 2-hr glucose is <140 (INDET), OR

 -  The 2-hour OGTT glucose is 140-199 mg/dl (impaired glucose tolerance, IGT).

 Exclusion Criteria:

 1. Diagnosis of CFRD, Consensus Conference definition (45)

 2. Previous organ transplant, or transplant imminent during study period

 3. BMI percentile >95

 4. Treatment with systemic glucocorticoids (nasal or inhaled glucocorticoids are
 acceptable)

 5. Therapy with growth hormone or Megace

 6. Nighttime continuous drip gastrostomy/jejunostomy feedings

 7. Pregnancy or breast-feeding or plans to become pregnant during study period

 8. Any change in medications during the 3 months prior to the study

 • Exception: the new corrector/potentiator combination drug lumacaftor/ivacaftor is
 expected to get FDA approval in early 2015, and most CF patients with severe
 genotypes, including many eligible for this proposal, will receive this drug. This is
 not a contraindication to participation in the current proposal (and participation in
 other studies is not contraindicated in the PROSPECT post-marketing drug study).
 Though the primary effects of the combination therapy appear to be apparent after 1
 month, we will wait 6 months after initiation of lumacaftor/ivacaftor before
 enrollment in this study to make sure subjects are in a steady state.

 9. Any anticipated change in medication during the 3 month study period

 10. Acute illness in the 6 weeks prior to enrollment