Non Invasive Diagnosis of Pneumocystis Pneumonia
Non Invasive Diagnosis of Pneumocystis Pneumonia
Incidence and morbi-mortality of Pneumocystis pneumonia (PCP) are increasing. Early and fast
 diagnosis and treatment improve PCP prognosis. Biological diagnosis is based on the detection
 of Pneumocystis jirovecii, mainly by PCR, in broncho-alveolar lavage (BAL) obtained from
 bronchial fibroscopy. However this invasive exam is not always possible in emergency in
 suspected patient and others non invasive (sputa) and/or non-targeted (bronchial aspiration)
 are sent to the laboratory (25% of cases, data from the Grenoble University Hospital).
 Diagnosis performances of these non invasive/non-targeted samples are not clearly
 established.

 In this study, the investigators aimed to establish the diagnosis value of non-invasive
 and/or non-targeted respiratory samples (oral fluids, sputa and bronchial aspiration) for the
 PCP diagnosis, compared to the gold-standard (Pneumocystis PCR on BAL, beta-D-glucans testing
 on serum and radio-clinical records).
Inclusion Criteria:

 -  Immunocompromised patient with (i) clinical and/or radiological suspicion of PCP, and
 (ii) bronchial fibroscopy with contributive BAL

 -  No immediate life-threatening conditions (estimated life expectancy >12h)

 -  No PCP treatment or PCP treatment < 48h

 -  Patient hospitalized in the Grenoble Alpes University Hospital with medical insurance

 -  Informed and written consent of the patient or its related

 Exclusion Criteria:

 -  Pregnancy, breastfeeding

 -  Exclusion period of another clinical trial

 -  Deprivation of liberty