Dipyridamole/Magnesium To Improve Sickle Cell Hydration
Dipyridamole/Magnesium To Improve Sickle Cell Hydration
The purpose of this study is to determine the benefits as well as side effects of giving
 drugs called dipyridamole and magnesium to patients with sickle cell anemia (SCA).
Vaso-occlusive episodes are the most common problem experienced by patients with SCA and the
 most frequent reason for hospital admissions as well as visits to the clinic and emergency
 department. Many cellular, humoral, and vascular factors influence the initiation and
 propagation of vaso-occlusion by sickle cells. Among these is the tendency of sickle cells
 (SS RBC) to become dehydrated with accompanying increase in the hemoglobin (Hb)
 concentration. Since sickle hemoglobin (Hb S) concentration controls the rate of
 polymerization, cellular dehydration plays a key role in sickle cell pathology.

 Two separate but interdependent cation transport mechanisms affect sickle cell hydration, the
 first involving abnormal KCl cotransport (KCC), and the second a sickle-induced (SI) passive
 leak which permits the influx of calcium ions (Ca++) that activates the Gardos pathway, a
 Ca++-dependent K channel. Early investigations aimed at inhibiting KCC with magnesium (Mg)
 and the Gardos pathway with clotrimazole met with partial success. We have recently shown in
 vitro that dipyridamole also inhibits the SI pathway. Strategies designed to block the
 formation of these dense, dehydrated cells would offer important therapeutic options that
 might decrease the number and severity of the vaso-occlusive episodes in patients. Drawing on
 the information gained from two decades of research on cation transport in SS RBC, including
 the unique discovery made at this Center that dipyridamole inhibits the SI cation leak, we
 now propose a study of combined therapy using two transport inhibitors aimed at reducing SS
 RBC dehydration.
Inclusion Criteria:

 -  Patients with homozygous sickle cell (Hb SS) confirmed by hemoglobin electrophoresis
 or HPLC

 -  Patients with adequate cardiac, renal, and liver function

 -  Patients with baseline fetal hemoglobin (Hb F) level of 10% or less

 -  Patients with at least 6% dense cells or higher at initial screening visit

 -  Patients with no history of coronary heart disease

 -  Patients with normal baseline ECG

 -  Patients with no history of hypotension or hypotensive episodes

 Exclusion Criteria:

 -  Patients who are pregnant, trying to become pregnant, or breast feeding

 -  Patients who are on a chronic transfusion program

 -  Patients who are unable to take oral medications

 -  Patients who have significant cardiac, renal, or liver dysfunction

 -  Patients who are on hydroxyurea

 -  Patients who have a fetal hemoglobin (Hgb F) level of greater than 10%, or have less
 than 6% dense cell on initial screen

 -  Patients who are taking a supplement which contains magnesium

 -  Patients who are taking aspirin, ibuprofen on a daily basis, or anti-coagulant such as
 Coumadin on a daily basis

 -  Patients who have a known underlying coagulopathy (acquired or congenital) or have
 prolonged PT or PTT at the time of initial screen

 -  Patients who have had a hypersensitivity to either of the study medications

 -  Patients who are taking any other study medication(s). Patients will not be excluded
 if they are on penicillin prophylaxis or folic acid, or use ibuprofen intermittently

 -  Patients taking tetracycline or sodium polystyrene sulfonate

 -  Patients on concomitant medications and other therapy must have a wash out period
 prior to study entry and/or study drug dosing

 -  Patients with abnormal baseline ECG

 -  Patients with a history of hypotension or hypotensive episodes