Effects of Proportioning Meat and Plant-based Protein-rich Foods on Cardiovascular Disease Risk Factors (S58)
Effects of Proportioning Meat and Plant-based Protein-rich Foods on Cardiovascular Disease Risk Factors (S58)
This project will assess the effects of consuming different proportions of red meat (RM) and
 plant-based, protein-rich foods (nuts, seeds, and soy products - NSS) incorporated into a
 U.S. Healthy Eating Pattern (HEP) on cardiovascular disease risk factors in adults at high
 risk of developing a heart-related disease.
Using a randomized, cross-over (balanced incomplete block) experimental design, Forty-eight
 middle-aged adults who are overweight and have high blood total cholesterol and LDL-C
 concentrations will be recruited. Participants will consume a HEP - all foods provided -
 during 5-week controlled feeding periods. The three HEP interventions will be: high RM, low
 NSS; moderate RM, moderate NSS; and low RM, high NSS. Each participant will complete two of
 the three controlled feeding periods, separated by four weeks when participants will consume
 their usual unrestricted diet (washout). The HEP consumed during the controlled feeding
 periods will be the same except for the amounts of RM (1, 5, or 9, 3-oz servings/wk) and NSS
 (high, moderate, and low, with amounts adjusted to isocalorically offset changes in RM energy
 intakes). Poultry, egg, and legume intakes will be the same among the three HEP. The
 investigators will measure clinically important cardiovascular disease risk factors before
 (usual unrestricted diet) and during the last week of each HEP intervention. The
 cardiovascular disease risk factors will include, but are not limited to, comprehensive lipid
 and lipoprotein profile (changes in low-density lipoprotein cholesterol, LDL-C; primary
 outcome), lipoprotein fractionation and particle numbers, and blood pressure. The
 investigators will compare improvements in heart disease risk factors and consumer
 satisfaction among the three HEP.
Inclusion Criteria:

 -  Male or female;

 -  Age 30-69 y;

 -  BMI: 25.0-37 kg/m2;

 -  Have hypercholesterolemia (total and LDL cholesterol 200-259 and 130-189 mg/dL,
 respectively),

 -  Systolic/diastolic blood pressure <140/90 mm Hg;

 -  Triglycerides <399 mg/dL, fasting glucose <109 mg/dL

 -  Body weight stable for 3 months prior (±3 kg);

 -  Stable physical activity regimen 3 months prior;

 -  Medication use stable for 6 months prior;

 -  Non-smoking;

 -  Non-diabetic;

 -  Not acutely ill;

 -  Females not pregnant or lactating;

 -  Participants must be willing and able to consume the prescribed diets and travel to
 testing facilities.

 Exclusion Criteria:

 -  BMI <25 or >37

 -  Total cholesterol >259 mg/dL, low-density lipoprotein cholesterol >189 mg/dL,

 -  Triglycerides >400 mg/dL, fasting glucose >110 mg/dL

 -  Body weight changes in previous 3 months (±3 kg)

 -  Changes in physical activity regimen in the previous 3 months

 -  Medication changes in the previous 6 months

 -  Smoking

 -  Diabetic

 -  Acute illness

 -  Pregnant or lactating