Effect of an Inhaled Corticosteroid on Airway Gene Expression in Asthma
Effect of an Inhaled Corticosteroid on Airway Gene Expression in Asthma
The purpose of this study is to determine whether an inhaled corticosteroid (fluticasone)
 alters the expression of any gene expressed in the lining of the airways of asthmatics. The
 study uses high density gene chips which allow the study investigators to measures all gene
 in the human genome. We hypothesize that this approach will identify novel genes that are
 affected by steroids in asthmatics.
This is a 10 week, randomized, double blind, prospective study comparing the effects of
 inhaled fluticasone or inhaled placebo on measures of airway function, airway remodeling and
 airway gene expression in asthmatic subjects. Enrollment has been completed as have all study
 visits. We are now in the data analysis phase. The study design was as follows: Following a
 one-week run-in/characterization period, subjects were randomized to receive 2 puffs BID of
 fluticasone (250µg/puff) or matching placebo for 8 weeks. Beginning with the run-in period,
 subjects recordes in a daily diary their peak flow measurements twice daily, (symptoms of
 cough, sputum production, wheeze, dyspnea, and chest tightness. They visited the laboratory
 for an interval diary review and spirometry and for medication dispensing. Bronchoscopy was
 performed at baseline (week 1 of the run-in), and 1 week after starting the study drug Weekly
 telephone contact will be made during the treatment period to monitor subject well being and
 to ensure compliance with study medication. There was a one-week run-out to allow monitoring
 of subjects after discontinuation of the study drug.
Inclusion Criteria:

 -  Male and female patients with a history of asthma between the ages of 18 and 70 years.

 -  PC20FEV1 Methacholine ≤ 8.0 mg/mL.

 -  At least one of the following symptoms, beta agonist use, or FEV1 criteria:

 -  Asthma symptoms on at least two days per week or Beta agonist use on at least two days
 per week or FEV1 < 85% predicted

 -  Subjects must be non-smokers (patients who have never smoked or patients who have not
 smoked for 1 year and have a total pack-year smoking history < 15 packs).

 Exclusion Criteria:

 -  History of oral or inhaled steroid use in the past 4 weeks.

 -  FEV1 < 60% predicted.

 -  Lung disease other than asthma.

 -  Patients with a history of a respiratory tract infection in the 4 weeks preceding the
 study.

 -  Patients who have experienced a significant exacerbation in their asthma in the 6
 weeks prior to the study.

 -  Patients receiving hyposensitization therapy with the exception of those who are on a
 stable dose for the last three months.

 -  Patients with cardiovascular disease (active) peptic ulcer disease or diabetes
 mellitus.

 -  Females who are lactating or who are pregnant.