A Biochip for Rapid Diagnosis of Complicated Urinary Tract Infection
A Biochip for Rapid Diagnosis of Complicated Urinary Tract Infection
The purpose of this proposal is clinical validation of an electrochemical biochip for rapid
 pathogen identification and antibiotic susceptibility determination.
Point-of-care identification of pathogens and determination of antibiotic susceptibility will
 significantly improve the clinical management of urinary tract infection. We have previously
 developed a biochip based on microfabrication technology capable of rapid detection of
 pathogens. The specific objectives of the current proposal are: 1) Determination of microbial
 constituents in spinal cord injury (SCI) patients and development of additional
 species-specific probes against these pathogens; 2) Development of a rapid antibiotic
 susceptibility and molecular pyuria assay using the electrochemical biochip; and 3) Clinical
 validation of the biochip as a diagnostic test for urinary tract infection.

 Within a single protocol, two non-interventional studies were conducted at different time
 points to achieve the aforementioned objectives. Sensitivity and specificity of the
 electrochemical biosensor based assay was demonstrated in each study.
Inclusion Criteria:

 -  Patients suspected or at risk for complicated urinary tract infections

 Exclusion Criteria:

 -  Gross contamination of urine samples at the time of collection