Microbiome and Sarcopenia in Patients With Liver Cirrhosis
Microbiome and Sarcopenia in Patients With Liver Cirrhosis
Protein-energy malnutrition (PEM) occurs in 65-90% of patients with liver cirrhosis. Severity
 of malnutrition correlates with progression of liver disease and leads to sarcopenia in
 30-70% of cirrhotic patients. Malnutrition and sarcopenia are associated with an increased
 risk of complications and mortality.

 In cirrhosis the gut microbiome is altered leading to increased gut permeability, bacterial
 translocation and inflammation. Since the microbiome is involved in nutrient uptake and
 metabolism, it is hypothesized that microbiome alterations contribute to sarcopenia. A
 prospective controlled cohort study to investigate the interrelation of microbiome changes
 and sarcopenia in cirrhosis will be conducted. Furthermore the effect of nutritional
 interventions on the microbiome in cirrhosis will be studied. From this study information on
 how the gut microbiome composition and sarcopenia are associated in cirrhosis and if
 modulation of the gut microbiome by nutritional interventions is feasible will be collected.
Scientific background

 Protein-energy malnutrition (PEM) occurs in 65-90% of patients with chronic liver disease.
 PEM is caused by various factors including poor dietary intake, loss of appetite, decreased
 hepatic protein synthesis, malabsorption and hypermetabolism. It is associated with an
 increased risk of complications including ascites, hepatic encephalopathy, variceal bleeding,
 hepatorenal syndrome and mortality. There is a direct relation between the progression of the
 liver disease and the severity of malnutrition.

 Malnutrition and sarcopenia in liver cirrhosis patients

 PEM leads to sarcopenia as a common, but frequently overlooked, complication. Sarcopenia is
 defined as a decrease in muscle mass two standard deviations below the healthy young adult
 mean. Sarcopenia is associated with aging, chronic diseases and malignancy. To determine the
 severity of muscle wasting, computed tomography scan (CT) or magnetic resonance imaging (MRI)
 are an objective and reproducible technique. Sarcopenia negatively impacts on survival,
 correlates with the risk of infections, increases surgical risk and leads to a poor quality
 of life. Besides PEM also inflammation is of importance in the development of sarcopenia.

 Diversity in the microbiome in patients with liver cirrhosis and association with sarcopenia.

 The gut microbiome of liver cirrhosis patients is altered compared to healthy individuals.
 Dysbiosis leads to an increased gut permeability, bacterial translocation and inflammation.
 This contributes to fibrogenesis and may also be related to hepatocarcinogenesis. Hence, new
 treatment approaches in cirrhosis focus on changing the microbial landscape.

 Modulation the gut microbiome may also be a strategy to reverse sarcopenia by reducing
 systematic inflammation.

 Hypothesis and aims

 There is an association between gut microbiome composition, gut permeability and the
 existence of sarcopenia in cirrhotic patients.

 Primary hypothesis: Diversity of the gut microbiome is reduced in liver cirrhosis patients
 with sarcopenia compared to those without sarcopenia or healthy controls.

 Secondary hypotheses: There is an association between gut microbiome composition, biomarker
 of gut permeability and bacterial translocation with the presence of sarcopenia in cirrhosis.
 Oral nutrition supplements (ONS) can influence the composition of the gut microbiome, gut
 permeability, bacterial translocation and inflammation. Sarcopenia can be diagnosed from
 patients portraits.

 Aims: to investigate:

 -  the composition of the gut microbiome

 -  biomarkers of gut permeability, bacterial translocation and inflammation

 -  the incidence and severity of sarcopenia

 -  the impact of oral nutrition supplements (ONS) on the gut microbiome

 -  whether artificial intelligence can be used to diagnose sarcopenia from face portraits.
Inclusion Criteria:

 -  Hospitalized patients for any reason with clinical/radiological/histological diagnosis
 of cirrhosis

 -  Age >18y

 -  Informed consent

 -  CT/MRI scan within +/-14 days of the baseline study visit

 Exclusion Criteria:

 -  Hepatic encephalopathy > grade 2 and or other cognitive disorder not allowing informed
 consent

 -  advanced hepatocellular carcinoma

 -  Any other condition or circumstance, which, in the opinion of the investigator, would
 affect the patient's ability to participate in the protocol