Study of Circulating Levels of Glicentin
Study of Circulating Levels of Glicentin
Hormones derived from proglucagon represent a family of peptides produced by the alpha cells
 of the pancreas and by the intestinal L cells. In the pancreas, the maturation of proglucagon
 mainly leads to the synthesis of glucagon, while in the intestine, the cleavage of
 proglucagon allows the synthesis of different peptides including glicentine, oxyntomodulin,
 Glucagon Like Peptide-1 (GLP-1) and Glucagon Like Peptide-2 (GLP-2).

 Glicentin is produced by L cells throughout the digestive tract, from the small intestine to
 the rectum, with a majority secretion in the colon. Studies in humans and animals have shown
 its role in the intestinal mucosa. It has a stimulating effect on the proliferation of the
 intestinal mucosa as well as an effect on smooth muscle cells and regulates trophicity and
 intestinal motility. Its circulating rate could be modified in case of intestinal ischemia.
 Mesenteric ischemia is a major diagnostic problem with high morbidity and mortality,
 particularly in the event of delayed treatment.

 The sensitivity and specificity of current markers are low. The identification of new
 biomarkers of the disease would improve the diagnosis and management of patients with the
 disease.

 The objective of the project is to determine a difference in circulating glicentin levels in
 patients with intestinal ischemia versus a control group.

 On this prospective monocentric study, 40 patients with digestive ischemia will be included
 in the Emergency Department of the University Hospital of Nice. A control group of 40
 patients with abdominal pain will be formed. The circulating glicentin levels will be
 measured on serum by Elisa technique at the Biochemistry Laboratory of the University
 Hospital of Nice, work that has been published in 3 scientific journals allowing us to
 develop and validate the technique.The staff will determine whether patients with digestive
 ischemia have impaired serum glicentin levels.

 The evaluation of the interest of new biological markers of mesenteric ischemia such as
 glicentine would constitute a definite diagnostic advance. This project could eventually
 offer new diagnostic and/or therapeutic perspectives in the management of these patients.
Inclusion Criteria:

 -  For patients with intestinal ischemia

 -  The combination of abdominal pain, altered general condition with abdominal defence
 and venous or arterial hyperlactatemia will be signs suggestive of a diagnosis of
 digestive ischemia.

 -  Patients suspected of digestive ischemia with the following comorbidities may be
 included as clinical suspicion and excluded post-operatively: emboligenic heart
 disease, arteriopathy, aortic and/or digestive atheromatosis.

 -  Patients with suspected digestive ischemia and functional scanning ileus.

 -  Patients with intestinal ischemia proven by CT scan with contrast injection: arterial
 abnormality such as dissection of an artery for digestive use, thrombosis or embolism
 of an artery for digestive use; intestinal or colonic thickening suggestive of
 reversible ischemic suffering of the digestive tract; parietal pneumatosis;
 gastrointestinal parietal enhancement abnormality (hypo-density ranges or total
 absence of enhancement).

 The following pathologies will be taken into account: mesenteric ischemia by embolism or
 thrombosis.

 -  Age over 18 years old

 -  Able to understand the study

 -  Affiliation to a social security system

 -  Signing of an informed consent

 -  Accept participation in the study (collection of a Non-Opposition)

 For the control group

 -  Age over 18 years old

 -  Able to understand the study

 -  Affiliation to a social security system

 -  Signing of an informed consent

 -  No personal history of colorectal cancer and obesity

 -  Admission to the Emergency Department of the University Hospital of Nice for abdominal
 pain with EVA > 3/10 of non-intestinal origin: nephritic colic, hepatobiliary
 pathology, gynaecological disease, etc.

 -  Abdominal CT excluding digestive tract pathology

 -  Accept participation in the study

 Exclusion Criteria:

 -  History of bariatric or digestive surgery (stomach, small intestine, colon or rectum)

 -  History of chronic inflammatory bowel disease

 -  Obese patients (BMI> 30kg/m2)

 -  History of type 1 diabetes or insulin treatment