The 2000 Oklahoma State Cowboys football team represented the Oklahoma State University during the 2000 NCAA Division I-A football season. They participated as members of the Big 12 Conference in the South Division. They played their home games at Lewis Field in Stillwater, Oklahoma. They were coached by head coach Bob Simmons, who resigned as the head coach of the Cowboys on November 6, 2000.
The 2000 OK State Cowboys had one of the best defenses in the Big 12. 