llluminating your books to greatness!
"As your ghostwriter, I listen carefully to your needs, stay true to your voice, illuminate your copy with methaphors, dialogue, humor and riveting poignancy, and can usually turn out your book in 4-5 weeks at a price far less than most ghostwriters!
For COMPUTER users, click the word "More" in the header above to view my various webpages! For those of you viewing on your PHONE, click the set of horizontal lines for the various pages.
A new comedy show by Pat Hurley!


Jonas Kopp was born in Buenos Aires, Argentina in 1981. His career as a producer was launched when he was 19 years old. After seven years of hard work we find one of the most representative argentine talents for the House and techno global scene.
Jonas is very well depicted by the constant hypnotic flavour in his tracks, the techno arrangements, deep basslines that really fill the surrounding space, the use of effects like reverb ,delays and LFO's and his faithful attachment to his Detroit and Chicago roots as creative influences .
His live performances are two hours of plenty of hipnotic groove, creating different states all along the set. His DJ sets are a perfect analog-digital fusion, using tracktor scratch and vinyls / Cds.. Artists such as Mattew Dear, Adam Beyer , Sven Vath , Chris Liebing, Par Grindvik, Ben Klock , Luke Slater , rank his tracks in their latest charts.
He founded Traut Muzik, a Label in which he curates the best techno and House artists from the argentine and global techno scene.


Gary Comer College Prep (GCCP), located in the Grand Crossing neighborhood on Chicago's south side, was conceived from the Comer Science and Education Foundation's core mission: to improve urban education and the lives of children. The 45,000-square-foot, state-of-the-art charter high school was opened in May of 2008 and is designed to be used in conjunction with the adjacent Gary Comer Youth Center.
The two-story structure is comprised of classrooms, a lecture room, and administrative offices for GCCP, to be operated by the Noble Network of Charter Schools in Chicago. The school shares facilities with the adjacent youth center, where students use the gymnasium, cafeteria, music rooms, art rooms, and computer lab space.
As of August 2014, GCCP is at full capacity serving 755 students in grades 9-12. GCCP students participate in youth center programming after school, on weekends, and during the summer.
Most classrooms include several interior and exterior windows providing excellent use of daylight and the benefits of an open design concept for students and staff. However, school administrators are responsible for providing a safe, stable and innovative learning environment for GCCP students. Parents count on GCCP leadership to be prepared for any situation where immediate privacy is needed.
– All the motorized shades are powered by ILT motors connected to the facility's data network, allowing staff access to the shades in the entire facility from any device connected to the network.
– All room-darkening fabric is No Lite Fiberglass, an opaque fabric with no view through.
– All fascia is 4″ white aluminum to match the classroom decor.
IWS designed a privacy shade system that is integrated with and controlled by the school's data network, which allows appropriate staff access to the privacy shades in the entire facility from any device connected to the network.
A total of 24 Illinois Window Shade Company motorized privacy shade systems were placed in classroom windows throughout the facility.
These systems are expertly fitted with No-Lite Fiberglass opaque fabric and seamlessly concealed by 4" white aluminum fascias to match the classroom decor.
The blackout shade successfully blocks all solar and visible light transmittance, providing a perfect environment for clients seeking optimum privacy levels or for those utilizing audiovisual equipment.
The privacy solution at Gary Comer College Prep looks fantastic, is easy for facility staff to operate and produces instant results with respect to the privacy and light levels in the facility.
Educational institutions and architects across the country are working to design sustainable solutions that provide enhanced security measures for school staff and students. The proper window shade addresses these challenges in a cost effective and practical manner. Well-designed motorized shading solutions not only save energy and money, but also provide another layer of privacy and security when needed.


After you see a Sports podiatrist Passaic NJ to your foot and ankle desires, you will see that you are going to sense lots higher.
it's miles estimated that people spend round four hours of every day on their ft and, in that time, they take about 8,000 to 10,000 steps. This all provides up to a mixed force equal to a few hundred tons an afternoon.
The reality is that a lot of us are reducing our toe nails incorrect.


text:
@interface CONStub : UICollectionViewController

- (id)initWithObject:(id)object originalSelector:(SEL)originalSEL stubSelector:(SEL)stubSEL;

- (void)andCallThrough;

- (void)andReturn:(void *)returnValue;

- (void)andCallFake:(id)block;

@end

redpajama_set_name: RedPajamaGithub


This is Sarge from Cars. I picked up this kids toy for €2,- and thought I might be able to use him for my USMC Army and now that he's done I'm not dissapointed.
The canopy is a bit off but doesn't bother me. I am going to use him as a transport jeep since he doesn't have a MMG. He also doesn't have an interior but the canopy is made of plastic and comes off easily so if you're a handy hobby man you could convert this model.
I just took off the headset (which was the hard part), filled some gaps with greenstuff and repainted him.
A Willy's jeep can transport up to 3 men so he comes in handy for transporting small weapon teams or a 1st or 2nd LT. Wheeled transports like these are a fast way to get your men where they are most needed.
First picture is the finished Jeep, second picture for size comparison and third pic is the original as I bought it.
Sarge is a character in Cars and Cars 2. His model is a 1942 or 1941 WWII (World War 2) Willy's Army Jeep. He is a WWII veteran.


Finished the 2013 Buller Gorge Recreational Walk in 03:22:44 in F5564 class!
She came 56th in class and 271st overall!
No photos of Trish Clothier in 2013 , we may still be uploading/processing, check back later.


text:
La saison 1989-1990 de Division 2 est la  du Championnat d'Algérie de football de deuxième division, regroupant 16 clubs. Au terme de la saison, les promus en Division 1 sont le WA Tlemcen et le CS Constantine.

Classement final
 Le classement est établi sur le barème de points suivant : une victoire vaut , un match nul 1 point et une défaite 0 point.
 Ce classement tient compte des forfaits de l'E Collo et du WAT (défalcation d'un point pour forfait conte MBB) ainsi que des matchs gagnés par ce dernier sur tapis vert respectivement contre sig (3-0) (incorporation de l'équipe de sig d'un joueur suspendu, score initial 1-0 pour CCS) et contre Aïn Oussera (3-0), (partie arrêtée à la suite de l'invasion du terrain par les supporteurs de l'équipe locale).
 Le samedi  le WA Tlemcen accède en national 1 à la suite de la décision de la Fédération algérienne de football (F.A.F.) d'attribuer le gain du match contre le CRB Ain Ouassera / WAT sur tapis-vert et sur le score (0-3).

Calendrier

Résultats

(*) CSC-USMB match retard joué le vendredi 3/11/1989.

(*) CSC-ASOC / OMM-USMB, matchs retards joués le lundi .

(*) IRBEH-WAT match retard joué le jeudi  à Annaba

(*) Le WAT ne c'est pas présenté au stade en guise de protestation au caillassage du car, transportant l'équipe, à l'entrée de la ville de Batna.

La FAF a jugé que ce retrait n'était pas justifié et sanctionnait le club tlemcenien d'un match perdu (0-3) et d'un retrait d'un point au classement.

(*) Le terrain de ES Guelma été suspendu.
(**) Le stade de Ain Ouassera été suspendu.
IRBH-GCM match retard joué le vendredi  à 14h00.

(*) CCS-WAT joué au stade opow  de Sidi Bel-Abbés en raison de la suspension du stade de l'équipe siguoise.

(*) CRBAO-WAT match arrêté à la  minute sur le score de 0-0 à la suite de l'envahissement du terrain par les supporteurs du CRBAO.
(**) CSC-OMM joué au stade  de Constantine, CRBAO-WAT joué au stade communal de Ain Ouassera, ESG-MBB joué au stade OPOW de Guelma, USMAB-JSMS joué au stade  de Ain Beida, CAB-GCM joué au stade OPOW de Batna .

|}

Meilleurs buteurs
Source

Notes et références
Archive de M'hamed.

Voir aussi ;
 Championnat d'Algérie de football 1989-1990
 Championnat d'Algérie de football de troisième division 1989-1990

Articles connexes

1989-1990
Algérie 1989-1990
Algérie 1989-1990
Sport en Algérie en 1989
Football en Algérie en 1990

redpajama_set_name: RedPajamaWikipedia


text:
Why the 'Catholicate is so precious to us' by Catholicos Baselius Marthoma Mathews I
Posted on July 17, 2016 by Orthodoxy Cognate PAGE
88th successor to the Holy Apostolic Throne of St. Thomas and Catholicos of the East and Malankara Metropolitan
MOTV– July 2016
'Why the Catholicate is so precious to us' by late lemented H.H. BASELIUS MARTHOMA MATHEWS I – CATHOLICOS OF THE EAST
Your Holiness Patriarch Ilya, Your Eminences, Your Excellencies, Your Graces, Honourable Ministers, and our beloved people.
Our heart is truly tilled with joy and gratitude on this occasion. Not only because God Almighty has been gracious to us, and has fostered this Catholicate here in India for the last seventy years. For that our gratitude to God knows no bounds.
But even more, our joy is fulfilled because you from our sister churches from all over the world have arrived here to share our joy. Ever since St. Thomas established this church here in the first century, the circumstances of history and geography have conspired to isolate us from other Christian Churches. Why did our sister church in China disappear? Mainly because of a similar isolation from the sister churches of the world.
The last seventy years mark not only the history of our re-established Catholicate but also a period of more helpful and more frequent contacts with sister churches all over the world. We remember especially the visit of one of our distinguished predecessors to the Faith and Order Conference in Edinburgh in 1935. We became founder members of the World Council of Churches, in 1948, and our bishops, priests and lay people have represented us in all important World Council meetings and other ecumenical gatherings. It is a matter of no small pride to us that the first Woman President of the World Council of Churches was our beloved daughter Miss Sarah Chacko, who later served the W.C.C. as its first Secretary for the Department of Cooperation between Men and Women and died in harness. Later our Fr. Paul Varghese served the W.C.C. as Associate General Secretary. Others like Dr. K. C. Joseph and Mr. C. I. Itty served on the staff in Geneva. The World Council of Churches became in many ways the forum through which our frequent and friendly contacts with the non-Roman churches were built up and maintained.
We are therefore particularly grateful that one of the Presidents of the World Council is present with us on this occasion – its Orthodox President H.H. Patriarch Ilya Catholicos Patriarch of all Georgia.
With the opening of the Second Vatican Council and from the time of pope John XXIII of blessed memory, our relations with the Roman Catholic Church also were put on a new basis of ecumenical co-operation. Our delegates were present at the Vatican Council as delegated observers. Our immediate predecessor had the privilege of greeting and embracing Pope Paul VI in Bombay. His Eminence Cardinal Willebrands, Fr. Pierre Duprey, Archbishop Jerome Hamer, Fr. John Long and others visited us, and opened and maintained a new set of genuinely sisterly relationships between our churches. It gives us particular pleasure that Pope John Paul II has delegated Fr. Pierre Duprey to represent His Holiness on this joyous occasion.
His Holiness Patriarch Athenagoras of blessed memory, that angelic ecumenical pastor, whom we never had the privilege of meeting, also maintained very close relations with our church and we are very happy to welcome the delegate of Patriarch Dmitrios his successor, in the person of Metropolitan Emilianos of Silibria.
All our distinguished international and Indian guests have already been welcomed. We wish only to add our personal welcome and gratitude to all of you.
In requesting to inaugurate the celebration of the 70th anniversary of the re-establishment in India of the Catholicate of the East, we wish to make one or two points clear.
First, ours is an autocephalous Orthodox Church of apostolic origin, and we want to develop our life in sisterly ecumenical co-operation with all our sister churches in the world. We are fully committed to the ecumenical movement – a prayerful dedication to make manifest our unity in Christ as His One Body. We are anxious to engage in active dialogue with our sister churches in India as well as abroad. We have officially communicated our desire to begin dialogue with the Catholic Bishops' Conference of India. We are still awaiting an official response. We have also communicated our desire to begin dialogue with the federation of the three evangelical churches: the Mar Thoma Syrian Church, the Church of South India and the Church of North India. We have now been in dialogue for some years with the Lutheran churches in India, and we are pursuing it. We hope the other two dialogues can also begin soon.
Secondly, I want to say a brief word about our relations with the Syrian Orthodox Church in the Middle East. Our experience in the past twelve years has been bitter. We have faced both Portughese and British intrigue and treachery in the past-instances in which our ecumenical hospitality has been sinfully misused to disrupt our Church from within. What the late Syrian Orthodox Patriarch did to our Church was something worse. We pray that God may forgive him. We allowed him inside our Church in 1958 in good faith. Since 1970 he consist ently betrayed that good faith and interfered in our internal affairs. His successor, the present Patriarch, came here recently, without even the courtesy of informing us, and began entering our churches and misleading our people.
He did not take one step towards a settlement of our disputes beyond certain very offensive paternalistic declarations of self-righteous willingness to forgive those whom his predecessor had hurt. We had cabled him before his departure from Syria to delay his visit in order that we could prepare the stage for a settlement of our disputes. To this date he has not had the courtesy to respond to our telegram.
We want to make this clear. We are anxious for a settlement. We are prepared to ask our deputies to sit down and discuss such a settlement with duly authorised people. We shall, of course, not compromise the autocephaly and independence of our Church. Nor can we allow any other church to interfere in the affairs of our church. Those are our two cherished values. We have sometimes compromised these values in the past for the sake of peace. We cannot do so in the future. We have learned our lesson.
We have a long history of association with our sister church of Antioch. We are grateful for their help on occasions in the past when exclusive heritage of the Orthodox Christians. St. Thomas belongs to the whole of India. In fact Jawaharlal Nehru once said whcn he was participating in the 1900 years' celebrations of St. Thomas, that St. Thomas belongs the whole Indian heritage. We are not making an exclusive claim of St. Thomas.
But there is a special point for this community. While for other Christians, it may be a part of history, while for some others it may be a part of heritage, for orthodox Syrian Christians, it is a part of their emotion, something which we have imbibed from the milk of mothers something which we want to transfer to our children and to our childrens' children as treasure to be preserved with awe and pride. This is the special feature about St. Thomas as far as we. Christians are concerned. I have always wondered why St. Thomas chose to come to this ancient Land of ours, which is the cradle for the four religions of the world, Hinduism, Jainism, Budhism and Sikhism and I have the answer for that.
In this ancient holy land of ours, Christianity out of necessity has to come from the authentic hands of the apostle of Christ himself. Imagine what would have been the inadequecy of Christianity, if our ancestors have been converted by the missionaries who came with the Portughese, the French or the Dutch. It was one of the justifications of the historical necessity and inevitable part of history that in this land, the authenticity of Christianity should be established by the disciple of Christ himself. When we speak about the authenticity of Christianity, we should also remember another thing and that is to preserve the faith of our fathers.
(Inaugural Address delivered by His Holiness Baselius Marthoma Mathews I Catholicos at the Sapthathi Meeting, Ernakulam)
This entry was posted in Featured News, News and tagged His Holiness Patriarch Athenagoras, Pierre Duprey, South India, Syrian Orthodox Church, World Council by Orthodoxy Cognate PAGE. Bookmark the permalink.

redpajama_set_name: RedPajamaCommonCrawl


text:
<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML3.2 EN">

<META NAME="GENERATOR" CONTENT="DOCTEXT">
<TITLE>MPI_Op_create</TITLE>

MPI_Op_create

Creates a user-defined combination function handle

Synopsis

int MPI_Op_create(MPI_User_function *function, int commute, MPI_Op *op)

Input Parameters

function
user defined function (function)

commute
true if commutative;  false otherwise. (logical)

Output Parameter

op
 operation (handle)

Notes on the user function

The calling list for the user function type is

 typedef void (MPI_User_function) ( void * a,
               void * b, int * len, MPI_Datatype * );

where the operation is <TT>b[i] = a[i] op b[i]</TT>, for <TT>i=0,...,len-1</TT>.  A pointer
to the datatype given to the MPI collective computation routine (i.e.,
<TT>MPI_Reduce</TT>, <TT>MPI_Allreduce</TT>, <TT>MPI_Scan</TT>, or <TT>MPI_Reduce_scatter</TT>) is also
passed to the user-specified routine.

Thread and Interrupt Safety

This routine is thread-safe.  This means that this routine may be
safely used by multiple threads without the need for any user-provided
thread locks.  However, the routine is not interrupt safe.  Typically,
this is due to the use of memory allocation routines such as <TT>malloc
</TT>or other non-MPICH runtime routines that are themselves not interrupt-safe.

Notes for Fortran

All MPI routines in Fortran (except for <TT>MPI_WTIME</TT> and <TT>MPI_WTICK</TT>) have
an additional argument <TT>ierr</TT> at the end of the argument list.  <TT>ierr
</TT>is an integer and has the same meaning as the return value of the routine
in C.  In Fortran, MPI routines are subroutines, and are invoked with the
<TT>call</TT> statement.

All MPI objects (e.g., <TT>MPI_Datatype</TT>, <TT>MPI_Comm</TT>) are of type <TT>INTEGER
</TT>in Fortran.

Notes on collective operations

The reduction functions (<TT>MPI_Op</TT>) do not return an error value.  As a result,
if the functions detect an error, all they can do is either call <TT>MPI_Abort
</TT>or silently skip the problem.  Thus, if you change the error handler from
<TT>MPI_ERRORS_ARE_FATAL</TT> to something else, for example, <TT>MPI_ERRORS_RETURN</TT>,
then no error may be indicated.

The reason for this is the performance problems in ensuring that
all collective routines return the same error value.

Errors

All MPI routines (except <TT>MPI_Wtime</TT> and <TT>MPI_Wtick</TT>) return an error value;
C routines as the value of the function and Fortran routines in the last
argument.  Before the value is returned, the current MPI error handler is
called.  By default, this error handler aborts the MPI job.  The error handler
may be changed with <TT>MPI_Comm_set_errhandler</TT> (for communicators),
<TT>MPI_File_set_errhandler</TT> (for files), and <TT>MPI_Win_set_errhandler</TT> (for
RMA windows).  The MPI-1 routine <TT>MPI_Errhandler_set</TT> may be used but
its use is deprecated.  The predefined error handler
<TT>MPI_ERRORS_RETURN</TT> may be used to cause error values to be returned.
Note that MPI does not guarentee that an MPI program can continue past
an error; however, MPI implementations will attempt to continue whenever
possible.

MPI_SUCCESS
 No error; MPI routine completed successfully.

See Also

 MPI_Op_free

Location:op_create.c


Did You Catch These Star Wars Easter Eggs?
Any big blockbuster movie is going to have a tonne of hidden details, easter eggs, and awesome little moments that you can watch a hundred times and still miss. But Star Wars: The Force Awakens, which has 40 years of fandom and history behind it and which is now officially the highest grossing movie of all time (domestically), probably has more Easter Eggs than any of those movies combined. Every time you watch it, there's something new to pick out—but sometimes you have to know where to look first.
"I haven't talked about it very much, but there is a scene where Rey's climbing around in the Star Destroyer, and as she balances on one of the beams and then leaps off the beam–in the shot where she leaps off, there's actually a cap with an Empire logo stitched on it, which is something that is really hard to see in the shadows," Tubach said.
There's something so satisfying about the way people get knocked off their feet when hit by blasters in Star Wars—and while the pew-pew shooty lasers look kind of retro now, they're definitely going to keep looking like that in the future.
Longtime fans of Star Wars have definitely seen MSE (or "mouse," as they're more frequently called) droids before, even if they didn't know it—they're these teeny tiny blocks of Imperial cuteness that roam the halls of the Death Star, and now the First Order. During your next viewing of The Force Awakens, watch very closely for these little guys, because they do some hilarious stuff.
There was also a practical mouse droid on set, as built by the guys who constructed the models of R2-D2 that were also used—and those guys wanted that droid to die real badly.
Not every Easter Egg is one that works in-universe. Like, for example, trees on Starkiller Base, which contain all sorts of secrets.
"When they built the snow set, in the barks of the tree there are certain shapes and images that the construction department and art department placed in there, which were all homages back to the original A New Hope." said Scanlan.


Around half of people suffering with drug addiction problems in Limerick are women.
That's according to the CEO of the Ana Liffey Drugs Project, as the group opens its new office in Limerick.
Their new home is located at Steamboat Quay.
Tony Duffin says there's still plenty of demand for Ana Liffey's services locally, with a higher proportion of women seeking help than elsewhere in the country.


T-Ball and A-Ball: Team placement assigned by the level commissioner.
AA Junior, AA Senior, AAA, and Majors: Team placement assigned by draft.
T-Ball and A-Ball: We do recognize that it is fun and comforting for younger children to be placed with existing friends. Therefore, requests for specific teammates and coaches are accepted at registration. Please note, though, that although we will make reasonable efforts to accommodate requests, we cannot guarantee that all requests will be honored. As a practical matter, if you request more than one or two teammates, it becomes more difficult to assemble teams if one of the players has been included with another person's request.
AA Junior, AA Senior, AAA, and Majors: Beginning at the AA Junior level, to help balance the competition, parent requests to have their child placed with another player are reviewed, but are less likely to be honored because of the priority placed on competitive balance.


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Tourism Minister Yariv Levin with Chinese tourists in the Old City of Jerusalem.
As the Jewish new year approaches, Tourism Minister Yariv Levin says there is good news to report, with tourist entries having increased by 16.5 percent since the beginning of this calendar year, injecting some $3.9 billion into Israel's economy.
According to the Central Bureau of Statistics, 278,800 tourist entries were recorded in August 2018, about 10 percent more than August 2017. In the period January – August 2018, about 2.6 million tourist entries were recorded, a 16.5 percent increase over the same period last year. Revenue from tourism in August reached about $414 million, and from the period January – August 2018, $3.9 billion.
"Month after month we are witnessing new achievements and record highs in incoming tourism," Levin says. "This is proof of the success of the innovative, marketing activities that we are implementing, together with the incentives for airlines and investments in infrastructure.
"The tourism industry continues to be a significant factor in making exceptional impact and contributions to the Israeli economy and workforce. I believe and hope that the new year will be a prosperous one for the tourism industry," he said.


text:
Efficient and Simple Aliquotting Automation
Sponsored Content by Andrew Alliance S.A.Jul 12 2019
In different fields, the term "aliquot" has different meanings: in chemistry and biology it refers to a smaller fraction of an entire sample. In the most part, an experiment only requires these fractions.
Therefore, aliquoting must be done before other productive tasks can be started. In some cases, aliquotting is just the simple transfer of the same volume from one source to numerous destinations. However, it can also involve a more complex distribution from numerous sources to numerous destinations, at different concentrations and different volumes.
The task can be further complicated if replicates are required for each different aliquot. As the complexity of the task increases, the hands-on time required from lab personnel also increases. More complex tasks also increase the risk of protocol execution errors and variability. Furthermore, repetitive aliquotting also exposes users to the risk of Repetitive Stress Injuries (RSI), as well as being costly and impacting on the quality of laboratory activities. There are many traditional automated liquid handling systems available currently.
However, systemic use of these is often neither accessible nor cost-effective for aliquotting activities. It is time-consuming to setup these expensive and complex devices and most automated liquid handlers need specific and expensive labware designed for automation. This is an unjustified extra expense if the aliquotting samples are to be used in manual experiments.
Aliquotting is probably used most commonly to extract the original stock samples into smaller portions. This means that different analytical tests of the same materials can be done, whilst at the same time preserving the original stock for future use. This is a daily occurrence at biobanks, where thousands of body fluid samples like urine, saliva and blood are delivered in tubes that need to be aliquoted into various cryo-vials.
Another common application is to aliquot master mixes of buffers and reagents during sample preparation for genetic and biochemical tests in molecular diagnostic, pre-clinical and forensic assays. These include PCR, RT-qPCR and ELISA. In these situations, aliquots are commonly taken from individual tubes and put into a range of smaller tubes, 384- or 96- well microplates.
Aliquotting can also be used to avoid repeated freeze-thaw or open-close cycle of reagents and this helps prevent changes in the physiochemical characteristics, for example heat-liable enzymes, easily oxidized chemistries of dNTPs, volatile solvents, delicate living cells and light-sensitive fluorescence conjugating moieties.
Only small aliquots of these reagents are required at a time, and so by using individual aliquots only when required, quality degradation is minimized. This in turn minimizes the negative consequences of failed experiments and the expenses that come with having to repeat work.
Most aliquotting applications only have one set volume that is dispensed repeatedly. However, some applications require volumes to be continuously changed. For example, lyophilized primer samples, which are normally synthesized at different quantities in microtubes, need to dissolved at different volumes to ensure that the concentrations of all primer stock solutions are equal. Another common example is the normalization of different protein, RNA or DNA samples to the same concentration. This requires aliquotting different volumes of buffer or water to the original sources or to different destinations.
Aliquotting variable volumes is particularly time consuming, tedious and prone to error. Human variability and simple errors in aliquotting can be very detrimental and lead to experimental results that are unusable and the waste of valuable reagents or samples.
Manual Aliquotting Completely Eliminated by Andrew
Automated aliquotting is the most convenient and obvious solution to eliminate the problems associated with manual pipetting, including pipetting mistakes, decreased lab efficiency, increased occurrence of RSI and working days that are too busy. However, most automated systems available currently are only compatible with limited consumables, specifically designed for each system, disregarding the variety of course and destination labware actually used in laboratories.
Consequently, additional transfer steps and reformatting of samples is required. Due to its highly adaptable working deck, the bench-top pipetting robot Andrew is the most suitable and affordable solution for small to medium throughput activities. The Andrew robot provides consumable flexibility that is not achieved with any other traditional robotic stations.
Andrew's working deck is made up of an innovative system of magnetic tiles, known as Domino blocks. These can be adapted to practically any type of regular consumable used in aliquotting tasks. As a result, Andrew can aliquot in almost any arrangement defined by users and also perform complex liquid handling tasks including cross-reactions, serial dilutions, array re-formatting, normalization, cherry picking and creation of master plates.
Andrew uses the same single channel pipettes present in most life-science laboratories and is operated from the user-friendly software, Andrew Lab. It only takes a few minutes to set up aliquotting protocols using Andrew Lab and this minimizes the time spent designing protocols. Furthermore, by importing and exporting data from and to ELNs, LIMS, or any other spreadsheet format, error-free traceability and documentation can be achieved.
How to Aliquot with Andrew? Three Examples
1. Aliquotting from One Source Tube to Multiple Destination Tubes with the Same Volume
One single aspirated volume of a solution can be dispensed rapidly in multiple destination tubes, using the "Repetitive" mode of Andrew. Andrew uses standard, classical mechanical pipettes to fill up the pipette tip to the highest capacity before dispensing multiple aliquots of the same volume one after the other, without touching the liquid. Therefore, it can return to source to refill for subsequent multi-dispensing cycles, without the risk of contamination. In order to ensure both precision and accuracy, Andrew discards the first and last aliquots of the dispensed series as these are normally affected by the largest error.
For instance, three 200 mL aliquots of a set stock sample can be made in one aspiration step using a 1000 mL pipette (Figure 1). The advantage of the repetitive mode becomes obvious when this principle is applied to 18 aliquots of 50 mL done in a single aspiration step: the time needed to aliquot compared to single dispensing is significantly shorter and consumption of tips is minimized (see the Andrew Alliance Application Note on Repetitive dispensing in Reference).
Figure 1: Schematic of the Repetitive pipetting mode of Andrew.
Two common procedures exemplify the productivity of Andrew in aliquotting from one source to multiple destination tubes. These procedures, preparing aliquots of urine and whole blood samples, are commonly performed in diagnostic labs (Figure 2). Using the virtual lab bench space in Andrew Lab, the mouse cursor is used to indicate the pipetting direction from the source tube "blood" to the first destination tube "cryo".
Figure 2: Example of an Andrew Lab protocol for aliquotting blood and urine samples with Andrew.
Holding down the Ctrl key whilst selecting additional tubes will include additional aliquotting targets. The aliquotting volume is then simply entered into the pop-up screen. The process can be repeated for aliquotting from the "urine" source tube to the four destination tubes "vial". This takes less than two minutes in Andrew Lab. Furthermore, protocols in Andrew Lab can be saved and used again for recurrent aliquotting tasks. This improves efficiency when handling lots of destinations.
2. Aliquotting from One Source Tube to Multiple Destination Tubes with Different Volumes
Another situation that is very common in the lab is aliquotting of variable volumes to multiple destination tubes from one source. Preparing primer stocks is a good example of this scenario. Synthesis yields vary between oligonucleotide sequences and experiments and so researchers need to dissolve the lyophilized primer stocks with different volumes of buffer or water so that they are all at the same concentration. The process becomes very simple and free from errors when Andrew Lab and Andrew are used.
The process begins with the creation of the desired consumables corresponding to the source (buffer or water) and destination vials in the virtual bench space of Andrew Lab (Figure 3A). This is followed by the pipetting step which can be accomplished easily, either by clicking on the "Pipetting" action from the "Add step" panel or by dragging the mouse cursor from the source tube to one of the destination tubes. Multiple volumes can be chosen by entering the volumes of buffer to be pipetted to each primer tube in the list, which is displayed after activating the "Multi-volume mode" option (Figure 3B).
Volumes can be entered in descendent or ascendant order to optimize time by minimizing the time needed for Andrew to change volumes and tips. By copying and pasting from a list previously designed in a spreadsheet or other document, the values can be introduced all at once (Table 1). The graphical and textual description of this aliquotting activity will be shown as a single instruction (Figure 3C).
Table 1: Primer list before and after being sorted according to volume.
Sorted Primer
Sorted Volume
primerR1 124 primerR1 124
primerR2 180 primerF3 140
primerF1 225 primerR2 180
primerF2 169 primerF5 187
Figure 3: Main steps in designing an aliquotting experiment from one source to many destination tubes in Andrew Lab.
Andrew can also be used to automate the resuspension step that follows. This is done by introducing a mixing action at the destination tubes, under the "Mixing" panel. The speed of mixing can be adapted (fast, medium or slow), to fit the experimental requirements or user preference. Introducing this mixing step can remove the need for the vortexing and centrifuging steps that are often required during resuspension of the primers. This substitution leads to better reproducibility and saves time. This aliquot protocol only took two minutes to design, and seven minutes to execute using Andrew (with the "on-the-fly" mode of pipetting activated).
For a total aliquot number of 100, the hands-on time increases to 10 minutes for protocol design and an additional five minutes to set up the consumables for Andrew. Manual aliquotting of 100 different volumes however, takes about 50 minutes of hands-on time to complete. Therefore, primer stick preparation using Andrew reduces hands-on time for repetitive aliquotting (using a pre-designed experimental protocol) by a factor of 10 (from 50 minutes to five minutes, Figure 4).
Figure 4: Workflow for the preparation of primer stocks either manually or by using the pipetting robot Andrew.
This is still the case, even if tubes need to be manually uncapped and accommodated using the Andrew physical work bench. Automation also allows more samples to processed in a reproducible and uniform manor.
3. Aliquot from many Source Tubes to many Destinations with Replicates
Technical replicates as well as biological repeats of the sample are critical in real-time PCR experiments. These replicates are essential for the establishment of the experimental error due to the amplification and analysis technique, and this allows confidence levels to be set for what is meant to be significant data. However, the preparation for the experiment becomes more cumbersome and complex because it involves aliquotting activities that are time consuming and need to be done extremely carefully.
Therefore, automating this task is desirable. Eight different DNA samples from four different biological conditions with two biological replicates each are tested in this example. For each sample, three technical repeats are included, resulting in the preparation of 24 aliquots in total, from variable sources (Figure 5). Moreover, these reactions need to be randomly distributed in a 96-well microplate in order to reduce bias error.
Figure 5: Experimental design of a real-time PCR experiment including three technical replicates per sample.
Similar to the example above, the volumes to be pipetted are sorted in order to reduce the volume changes, and consequently the execution time of the experiment. Next, the source and destination tubes are dropped in triplicates from the virtual bench to the consumable section (Figure 6A). Then, the two lists, of the destination well positions and volumes, are copied and pasted into the corresponding Andrew Lab fields (Figure 6B).
All of the 24 aliquot pipetting steps are included in one instruction (Figure 6C). Normally, Andrew will change the tip for each pipetting step. However, if the user wishes to reduce consumable costs, fewer tips can be used (for example one single tip for each set of triplicates). In this instance, the protocol can be designed with an independent pipetting instruction for each sample, and this gives the user full control over tip choice for the aliquotting of each sample (Figure 7).
Figure 6: Design of an aliquotting experiment involving many sources and many destination wells in Andrew Lab. A) Source and destination tubes are dropped in the Andrew Lab virtual bench. B) The list of volumes to be pipetted and destination wells positions can be directly introduced or simply pasted from a spreadsheet into the Andrew Lab columns dedicated for this information (green highlighted area). C) At the end of this procedure, all pipetting steps are consolidated in a single instruction (yellow highlighted area).
Figure 7: Aliquotting triplicates in an independent pipetting step for each source tube with Andrew Lab.
If the sources also come from multiple wells in another microplate, or even in the same microplate, the entire source plate can be dropped into Source. The sorted list of source well positions, corresponding to the correct aliquot volume, can then be copied and pasted during the Configuration step. If a consumable representing a rack of tubes in any format is dragged onto the virtual bench, the same procedure can be followed. This makes designing the experiment easier and faster.
This experiment takes about 5.5 minutes to design in Andrew Lab, if the tips are changed for every single aliquot. Andrew completes this protocol fully unattended and with no mistakes in only 10 minutes. Although executing the protocol manually may take approximately the same amount of time, manual execution is prone to possible errors and is entirely dependent on the operator.
The robot Andrew improves aliquotting efficiency significantly by fully eliminating human variations and errors, minimizing labor time, adapting to a diverse range of labware and integrating multiple aliquotting strategies. The intuitive Andrew Lab software facilitates simple-to-design protocols and improved documentation and traceability of the aliquotting steps and materials.
In addition, the personal health of the user is protected by avoiding the direct handling of chemically or biologically hazardous liquids and the risk of developing muscular disorders such as RSI is reduced (see the Andrew Alliance Application Note on pipetting ergonomics in Reference).
Q.A. Ngo. Automatic accurate repetitive pipetting with standard pipettes by Andrew robot. Application Note No. 6 – 20160418 v3. Andrew Alliance. 2016.
J. Denyes & N. Pennese. Pipetting Ergonomics: risks and solutions. Application note No. 1 - 20160912 v2. Andrew Alliance. 2016.
About Andrew Alliance S.A.
Andrew Alliance is an independent, privately financed company, based in Geneva, Boston and Paris. The company was created in March 2011.
Andrew Alliance is dedicated to advance science by working with scientists to create a new class of easy-to-use robots and connected devices that take repeatability, performance, and efficiency of laboratory experiments to the level required by 21st-century biology.
Start with meeting customer needs, end with customer feedback.
Andrew Alliance delivers solutions that are focused on customer needs, both today and in the future. Our products are manufactured to the highest standards, using a range of carefully selected, proven, and sustainable technologies, that ensure both high performance and reliability. We actively seek continuous customer feedback, in order to guarantee the best possible design outcomes.
Sponsored Content Policy: News-Medical.net publishes articles and related content that may be derived from sources where we have existing commercial relationships, provided such content adds value to the core editorial ethos of News-Medical.Net which is to educate and inform site visitors interested in medical research, science, medical devices and treatments.
Last updated: Jul 22, 2019 at 7:57 AM
Andrew Alliance S.A.. (2019, July 22). Efficient and Simple Aliquotting Automation. News-Medical. Retrieved on January 23, 2020 from https://www.news-medical.net/whitepaper/20190712/Efficient-and-Simple-Aliquotting-Automation.aspx.
Andrew Alliance S.A.. "Efficient and Simple Aliquotting Automation". News-Medical. 23 January 2020. <https://www.news-medical.net/whitepaper/20190712/Efficient-and-Simple-Aliquotting-Automation.aspx>.
Andrew Alliance S.A.. "Efficient and Simple Aliquotting Automation". News-Medical. https://www.news-medical.net/whitepaper/20190712/Efficient-and-Simple-Aliquotting-Automation.aspx. (accessed January 23, 2020).
Andrew Alliance S.A.. 2019. Efficient and Simple Aliquotting Automation. News-Medical, viewed 23 January 2020, https://www.news-medical.net/whitepaper/20190712/Efficient-and-Simple-Aliquotting-Automation.aspx.
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You've achieved a strange level of irony, expressing admiration for Killer7, yet concern that Red Steel 2 might be too linear.
Mafia 2 will be the best game of 2010, mark my words : ).
I agree with lot of those - Fallout New Vegas is my current number 2 anticipated game, the third one is fifth Hitman.I wish they would finally show him, but I guess they Eidos will now concentrate on the new Batman game.
But since the release dates for Hitmans were 2000,2002,2004,2006, and now it is 09 and the game is not even announced, I wonder what takes so long.Kane and Lynch was made by second internal team I think.
I stopped completely in love at the last guardian. How charming is that?
I just want to jump right in and play.


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Pico4ML Brings Machine Learning To the Raspberry Pi Pico
By Les Pounder published 10 March 21
Tiny Machine Learning Platform
(Image credit: Arducam)
The Raspberry Pi Pico wouldn't be the first board that comes to mind for machine learning, but it seems that the $4 may be a viable platform for machine learning projects. The Pico4ML from Arducam is an RP2040 based board with an onboard camera, screen, and microphone that looks to be the same size as the Raspberry Pi Pico.
Arducam is probably better known for its range of cameras for the Raspberry Pi and Nvidia Jetson boards, but since the release of the Raspberry Pi Pico, they have been tinkering with machine learning projects powered by the Pico. The Arducam Pico4ML is their first RP2040-based board and the first board to feature an onboard camera, a microphone that you can use for "wake word" detection, a screen, and an Inertial Measurement Unit (IMU) that can detect gestures.
The Pico4ML is intended for machine learning and artificial intelligence projects based around Tiny Machine Learning (TinyML). The TensorFlow Lite Micro library has been ported to the RP2040, opening up a whole new world of projects for the $4 microcontroller. The Arducam Pico4ML is at its heart still a Raspberry Pi Pico, and so it should be compatible with accessories designed for the Pico.
The Pico4ML can detect two persons in real-time, and in the latest demo video, we see it in action with a real person and a Mario action figure. Pico4ML reacts with a percentage value to show how certain it is that an image is a person while providing a live camera feed of the subject in the frame.
At this time we don't know how much and when this board will be available, but we do know that Tom's Hardware will receive one for review in the next few weeks.
Les Pounder
Les Pounder is an associate editor at Tom's Hardware. He is a creative technologist and for seven years has created projects to educate and inspire minds both young and old. He has worked with the Raspberry Pi Foundation to write and deliver their teacher training program "Picademy".
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