text:
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Polymerase Chain Reaction And Gene Therapy
The role of polymerase chain reaction(PCR) and Gene therapy in medicine.
PCR was developed by Kary Mullis in the 1980s. It is a method in which a single copy of DNA is amplified. Using this technique which has a specific sequences ,millions of copies of DNA can be produced. In this process, many other conditions including several enzymes and primers are involved. This application of PCR extent from research to the commercial sector. Denaturation:-
The reaction mixture is heated up to 367 to 371 Kelvin for 20 to 30 minutes. It produces the softening of the double-stranded DNA by cracking the hydrogen bonds which holds the two templates together. Then the DNA molecule turns into a single-stranded one.
Annealing:-
When the DNA strands …show more content…
For this purpose, Taq polymerase is used often.
Elongation:-
A temperature of 343 to 347 Kelvin is usually necessary for this step to make sure complementary strands have been founded by all the single-stranded DNA templates .
Advantages of PCR technique.
1.Amplifies DNA: PCR is a great technique for DNA testing.Very tiny samples are being used for identifying the DNA of individuals. This device helps in amplifying whichever small amounts of the DNA attained.
2. Law enforcement: PCR is the best technique for crimes and legal investigations in which only a small amount of DNA are acquired.PCR duplicates the samples available through transcription.It develops the chance of recognizing the source of the DNA samples. Utilizing the technique of PCR, many offenses have been solved prosperously.
3. Better HIV test: Since PCR is more sensitive and accurate successfully, it is also quite useful in HIV testing. Rather than searching for the presence of HIV antibodies, PCR directly gazes for pro-viral DNA. At 29 days from a surmised exposure or contact ,the complexity of this test makes it 98% accurate.
Limitations of polymerase chain reaction …show more content…
If reverse PCR is done,then living organisms can be identified. Examples; diagnosis of viral infections like cytomegalovirus, hepatitis , and Influenza (flu).
2.Medico-legal cases:
PCR permits the Deoxyribonucleic acid(DNA) from a blood cell or hair follicle to be examined.Example: suspected homicides,suicides,and cases referred from the court.
3.PCR application in the identification of genetic disorder:
The Polymerase chain reaction technology has been most commonly used to amplify the gene segments which contain kenned mutations for diagnosis of inherited diseases like beta thalassemia, sickle-cell anemia,cystic fibrosis,etc.
4. PCR is especially useful for the prenatal diagnosis of inherited diseases where cells obtain from fetus by amniocentesis,etc.
5.Detection of infectious diseases; Example: tuberculosis,AIDS.
Principles of gene therapy.
The aim of gene therapy is to introduce the transgenes or gene into appropriate target cells to restore normal function, therefore, treating and preventing the disease .Similar technologies are being used in gene immunotherapy and DNA vaccines against cancer and other infectious diseases. For a long period of time, current focus with gene therapy is the need for high level of gene expression and to minimize safety issues such as immune reaction against transgene product or transfer vectors and associated cancer
Polymerase Chain Reaction (PCR)
Extension - temperatures of the reaction is raised to extend the primers and synthesize new strands of DNA by Taq polymerase. This is carried at a temperature of 720c (161.50F). The cycle is repeated by denaturing and synthesizing the new DNA strands with multiple copies. PCR cycle takes few hours if the cycle is automated. The thermocycler is a directed machine to program the alteration of the temperature in the reaction for denaturing and synthesizing.…
Words: 916 - Pages: 4
A Polymerase Chain Reaction (PCR)
• The fragments undergo autoradiography where they are revealed to DNA probes—pieces of synthetic DNA that are made radioactive. • An X-ray film the fragments. When a radioactive probe attaches to the fragments, a dark mark will be displayed. This pattern of marks is analyzed. Applications • Used for the paternity…
Analysis Of The Origami Of Dna
The article had a lot to do with DNA and genes and how looping and folding of other.DNA allows cells to function correctly. Cells are basically a huge map for proteins and different things. In the Article shows how there were a lot of experiment taking place into the origami of DNA.Another Experiment that took place in the article was the HI-C method they found that there was 4.9 billion points in the genome in one segment. Looping were combine with histone that have been turn on by genes. At the same time the loops contained tiny parts where a protein is called CTCF.Researcher found large parts of gene looped together.…
Influence Of Genetic Differences In Living Organisms
As the DNA is unwound, this creates a replication fork. Since replication occurs in a bi-directional way, a leading and lagging strand are produced. The leading strand is made in the same direction as the replication fork, nucleotides are added continuously. The lagging strand is made in the opposite direction of the replication fork. This discontinuous synthesis of DNA causes short segments of DNA called Okazaki fragments to be formed.…
Words: 1415 - Pages: 6
Cancer Immunotherapy Research Paper
Researchers have developed monoclonal antibodies (drugs) for CTLA-4 to activate the CD28/B7 T cell activation and elicit an immune response against tumors (Alegre & Fallarino 2006). Monoclonal antibody therapy is a type of immunotherapy that recognizes and attaches to cells or proteins. It can stimulate the immune system to attack cancer cells. The most recent monoclonal antibody drug approved for metastatic melanoma is…
Dna Synthesis Essay
The nucleotides is basically the place where the synthesis of a new strand will be made. Each nucleotide has only one base. Which would either be A, T, G, or C. These bases tells you the code of DNA which tells you the sequence of the…
Plasmid Isolation Lab Report
The thermocycler is where PCR will occur. It consists of several cycles, about 35 cycles that amplify a single DNA sequence to over 2¬¬¬35 copies. Once the DNA is amplified, it can be analyzed on a gel. My products in the +RT tube were analyzed; as expected I found a bright band around the 1100 bp, indicating that my amplified sequence is as expected. However, in the –RT, a dim band was found around the 1300 bp, indicating a possible genomic DNA contamination.…
Dna Synthesis
At this stage, the main objective is unwinding the double-helical structure of the DNA as proposed by Watson and Crick in 1953 and then use both strands as a template to continue the replication process (Weaver, 2012). According to Lodish (2008), it begins with the enzyme helicases steadily unravelling the strands at the replication origins by breaking the hydrogen bonds between the nitrogen bases of the DNA and continue to form a replication fork as it separates the strands into two parental strands (Reece, Reece & Campbell, 2011). During this time, Reece et. al (2011) explain that to prevent the recoiling of the strands, single strand binding protein firmly attached to the free-bound DNA nucleotides, hence inhibiting the formation of hydrogen bonds between the nitrogen bases of the DNA. Subsequently, since the action of unravelling and untwisting the helixes create supercoiling, a torsional pressure along the replication fork, topoisomerase is fully utilized to subdue the supercoiling (Lodish, 2008).…
What Is Oyster Aquaculture?
Agarose gel electrophoresis is the common and easy way to analyze DNA where that DNA is visualized in the gel. There is a problem may be occur during PCR reaction and electrophoresis such as DNA product appear variable in non-specific on agarose gels. Sometimes no product form at all. (Lorenz,2012). Other is mutation occur during amplication that result in a heteregoneous population of PCR products.…
DNA Modification In Genetic Engineering
There are many different methods of DNA modification under testing, but two processes in particular have been gaining attention in the medical world. The first is that of Luhan Yang who was mentioned earlier. Yang and her colleagues have been making crucial headway on an innovation called CRISPR. CRISPR involves replacing parts of the DNA with proteins and is already widely used by biologists as a tool to alter DNA down to the level of a single letter (Pak). Its precision has the potential to offer the best approach for gene therapy in people with destructive illnesses.…
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Dragon Stompy is a tried and true strategy from the world of Legacy, but Suczi is taking it to the next level using this build to dominate a recent Classic Premier Event. The premise of the deck is simple: use accelerated mana in the form of Ancient Tomb, City of Traitors, and temporary mana effects like Seething Song to speed out a super-fatty or super-disruptive spell as early as the first turn. Many a mage's blood has turned cold at the sight of a first turn Magus of the Moon shutting down their deck's manabase.


text:
using System;
using System.Collections.Generic;
using System.Linq;
using System.Text;
using System.Reflection;
using Newtonsoft.Json.Serialization;
using Newtonsoft.Json;
using System.Collections.Specialized;

namespace Bocai.Common.Utilities
{
    /// <summary>
    /// 重写属性名称方法。增加压缩版实体解析
    /// </summary>
    class PropertyNameContractResolver : DefaultContractResolver
    {
        /// <summary>
        /// 是否启用自定义名称
        /// </summary>
        private readonly static bool isUseCustomName = (System.Configuration.ConfigurationManager.AppSettings["isUseCustomName"] ?? "0") == "1";

        /// <summary>
        /// Creates properties for the given <see cref="JsonContract"/>.
        /// </summary>
        /// <param name="type">The type to create properties for.</param>
        /// /// <param name="memberSerialization">The member serialization mode for the type.</param>
        /// <returns>Properties for the given <see cref="JsonContract"/>.</returns>
        protected override IList<JsonProperty> CreateProperties(Type type, MemberSerialization memberSerialization)
        {
            List<MemberInfo> members = GetSerializableMembers(type);
            if (members == null)
                throw new JsonSerializationException("Null collection of seralizable members returned.");

            JsonPropertyCollection properties = new JsonPropertyCollection(type);

            foreach (MemberInfo member in members)
            {
                JsonProperty property = CreateProperty(member, memberSerialization);
                if (property == null)
                {
                    continue;
                }
                if (isUseCustomName)
                {
                    property.PropertyName = property.UnderlyingName;
                }
                property.PropertyName = FormatStringForJava(property.PropertyName);
                properties.AddProperty(property);

            }

            IList<JsonProperty> orderedProperties = properties.OrderBy(p => p.Order ?? -1).ToList();
            return orderedProperties;
        }
        /// <summary>
        /// 格式化变量名,提供给java规范使用
        /// </summary>
        /// <param name="value"></param>
        /// <remarks>扩展方法必须在非泛型静态类中定义</remarks>
        /// <returns></returns>
        private string FormatStringForJava(string value)
        {
            string newValue = string.Empty;
            if (value.Length > 0)
            {
                newValue = value.Substring(0, 1).ToLower() + value.Substring(1);
            }
            return newValue;
        }

    }
    /// <summary>
    /// json序列号助手
    /// </summary>
    public static class JsonHelper
    {
        /// <summary>
        /// 是否启用压缩版本
        /// </summary>
        private readonly static bool isZip = (System.Configuration.ConfigurationManager.AppSettings["isZipJson"] ?? "0") == "1";

        /// <summary>
        /// Json序列化设置
        /// </summary>
        private static JsonSerializerSettings settings = new JsonSerializerSettings { ContractResolver = new PropertyNameContractResolver() };

        /// <summary>
        /// 序列化对象
        /// </summary>
        /// <param name="obj"></param>
        /// <returns></returns>
        public static string SerializeObject(Object obj)
        {
            Formatting f = isZip ? Formatting.None : Formatting.Indented;
            return JsonConvert.SerializeObject(obj, f, settings);
        }
        /// <summary>
        /// 反序列化对象
        /// </summary>
        /// <param name="json">json字符串</param>
        /// <param name="type">实体类型</param>
        /// <returns></returns>
        [Obsolete("使用泛型方法,DeserializeObject<T>")]
        public static object DeserializeObject(string json, Type type)
        {
            return JsonConvert.DeserializeObject(json, type);
        }
        /// <summary>
        /// 反序列化对象
        /// </summary>
        /// <typeparam name="T">对象类型</typeparam>
        /// <param name="json">json字符串</param>
        /// <returns></returns>
        public static T DeserializeObject<T>(string json)
        {
            return JsonConvert.DeserializeObject<T>(json);
        }
    }

    /// <summary>
    /// 给Form表单数据提供序列化的方法
    /// </summary>
    public static class JsonExtensionForNameValueCollection
    {
        /// <summary>
        /// 将From表单参数转化成对象数组容器
        /// </summary>
        /// <param name="nvc">Form表单</param>
        /// <returns>对象数组</returns>
        public static Dictionary<string, object> ToDictionary(this NameValueCollection nvc)
        {
            var result = new Dictionary<string, object>();
            foreach (string key in nvc.Keys)
            {
                string[] values = nvc.GetValues(key);
                if (values.Length == 1)
                {
                    result.Add(key, values[0]);
                }
                else
                {
                    result.Add(key, values);
                }

            }
            return result;
        }
        /// <summary>
        /// 将实体转换成传输的POST字符串
        /// </summary>
        /// <param name="nv">请求实体</param>
        /// <returns>字符串</returns>
        public static string ToQueryString(this NameValueCollection nvc)
        {
            Dictionary<string, object> list = nvc.ToDictionary();
            StringBuilder queryStr = new StringBuilder();

            if (list != null)
            {
                foreach (var k in list)
                {
                    queryStr.AppendFormat("&{0}={1}", k.Key, k.Value.ToString());
                }
            }
            return queryStr.ToString().TrimStart(new char[] { '&' });
        }
        /// <summary>
        /// 将From表单参数序列化成Json字符串
        /// </summary>
        /// <param name="nvc">Form表单</param>
        /// <returns>对象数组</returns>
        public static string ToJsonString(this NameValueCollection nvc)
        {
            return JsonHelper.SerializeObject(nvc.ToDictionary());
        }
    }

}

redpajama_set_name: RedPajamaGithub


Regular updates from the world of luxury watches with just one click.
Panerai celebrates the french-diving champion Guillaume Néry with its pre-SIHH launch, the new Submersible Chrono - Guillaume Néry edition.
Guillaume Néry, an ambassador of the sea, two-time world champion and four-time record-holder for weight free-diving, is also a true paneristi. The Florence-based brand, Officine Panerai, celebrates this ambassador with the latest addition to their Submersible family, the Submersible Chrono - Guillaume Néry edition.
Resonating the brand's nautical past, this edition is a true professional diver's watch with its trademark unidirectional rotating bezel and luminous white markers. Water-resistant to 300 meters, the timekeeping instrument comes in a titanium case which integrates well with the blue ceramic bezel, the blue hands and the blue rubber strap.
The Submersible Chrono – Guillaume Néry edition comes fitted with a P.9100 automatic calibre, housing a power reserve of three days. The 47mm chronograph also comes with a flyback function and the device for zeroing the seconds hand to enable perfect synchronisation with a reference signal.
Phillips presents the "Don Pancho"
Launched in 2012, WatchTime India is the result of a collaboration between America's most-read watch magazine, WatchTime and, India's leading media house, Malayala Manorama. With an aim to popularise and celebrate the evolving watch culture of the country, the publication is your one-stop destination for everything related to fine luxury watches. From the latest tests to reviews, to exclusive features on the history and horological heritage of some of the most spectacular watch brands of the world, the WatchTime India portal has a lot to offer. Stay tuned for an exciting journey, through the fascinating world of watches!
Monthly home delivery, with free shipping of the print issues. Plus an exclusive access to the online archive on our E-Magazine edition!


Q: What does your spa pedicure include?
If you're looking for relaxation, this is it! Soak in an amazing foot spa, toes, cuticles, foot file (reduction of callus), foot scrub, foot massage all included. Sit back and enjoy a luxury pedicure station complete with a side table to put your beverage or magazine as well as a fabulous full back massage.
Q: What is the difference between your spa pedicure and your mini pedicure?
With a mini pedicure you get everything listed above except the foot file and foot scrub. The mini pedicure is great for the budget, if you don't have the time for a full hour pedicure, or if you still want the joys of a pedicure but don't need a foot scrub or file.
Q: What is a gel manicure?
Gel polish is the only difference. Gel polish is a gel but it is soakable and is not an enhancement (you can not add length with this product). The wonderful part about gel polish is you will leave dry and stay shiny! The product will stay (and still shine) for up to 2 weeks. No drying time, no worries about smudging! Who wouldn't want that convenience?
Q: Do you offer gel polish changes?
NO the removal and application process for a gel color change is the same length of time and procedure as a gel manicure.
Q: How many nail and gel polishes do you have?
A lot....over 200, so if you can't decide we'll be more than happy to assist. Still can't make up your mind? Well then it's a good thing it's not a tattoo ;) You can choose to view the polish on the rack or browse through those same polishes on a color palette where you will see the way it will really come out once it is on your nails.
Q: What brand of gel and acrylic products do you use?
Only the very best and that would be - Young Nails. Winner of 2009 best Acrylic and Gel by Reader's Choice in Nails Magazine. We also carry Cuccio, China Gelaze, Orly, and QRS.
Q: What brand of nail polish do you use?
Q: How often should I come in for a gel manicure and or gel/acrylic fill?
We suggest every 2 weeks.
Q: How often should I come in for a pedicure?
Summer time - plan to come in about every 4 weeks. During the winter it will be roughly every 6-8 weeks. Yes, you should take care of your feet all year - they take care of you, don't they?
Q: What is the best way to prepare for a pedicure?
Q: Does keeping gel polish or any enhansment on for to long damage your nails.
NO! Not at all. I have said this for over 15 years. It's not the tool its the fool! If the product being used is a professional product it will not do any damage to your nails. It's the over filing of the nail that does the damage. Make sure your nail professional is well trained and you will be just fine.
Yes, we accept walk-in's. However, we may not always be able to accommodate you at that time. It is always best to call ahead or make an appointment.
Q: How long does it take for a pedicure?
A spa pedicure takes about 1 hour and a mini pedicure takes about 30min.
Q: Do you sell OPI nail polish?
We sell Caption Polish and nail strengtheners.
Q: Should I shave my legs right before my pedicure?
Q: I am a nail/cosmetology student/professional. Can you teach me?


Exclusively available at BB.com. Four wear everywhere bands can be mixed and matched with everything in your repertoire. Two simple slender gold bands are complemented by an organic band and a cool chain link making this the perfect set of four. EVERYDAY FINE COLLECTION: Hand-crafted using 18K gold and sterling silver, it stands up to wear, tear, and tarnish better than many wildly expensive brands. Items come complete with a luxe black storage pouch.
18K gold plated sterling silver casting.
1st band width: .09". 2nd band width: .06". 3rd band width: .04". 4th band width: .02".


Receiving a diagnosis of cancer can have a huge impact on most people. Feelings of depression, anxiety, and fear are very common and are normal responses to this life-changing experience. These feeling can result from changes in body image, changes to family and work roles, feelings of grief at these losses, and physical symptoms such as pain, nausea, or fatigue. People might also fear death, suffering, pain, or all the unknown things that lie ahead. But, cancer diagnosis is not a death sentence. Over half of the people diagnosed with cancer are still alive 10 years later and this number is rapidly improving. It is estimated that 14,483,830 adults and children with a history of cancer alive in the United States today. So, there are a vast number of cancer survivors.
Surviving cancer carries with it a number of problems. "Physical, emotional, and financial hardships often persist for years after diagnosis and treatment. Cancer survivors are also at greater risk for developing second cancers and other health conditions." National Cancer Survivors Day. Unfortunately, most of these residual problems go untreated. Psychologically, cancer survivors frequently suffer from anxiety, depression, mood disturbance, Post-Traumatic Stress Disorder (PTSD), sleep disturbance, fatigue, sexual dysfunction, loss of personal control, impaired quality of life, and psychiatric symptoms which have been found to persist even ten years after remission.
Zhang and colleagues examine the published research literature investigating the effectiveness of mindfulness training for anxiety and depression in cancer patients. They report that the most common form of mindfulness training used in the published research for cancer were 8-week Mindfulness-Based Stress Reduction (MBSR) programs involving meditation, body scan and yoga practices. All studies examined contained a control condition, most commonly a treatment as usual wait-list group.
They found that the literature made a clear case that mindfulness based therapies produce significant improvements in both anxiety and depression in the cancer patients. Since mindfulness training involves training to focus on the present moment, it is easy to see how it could be effective against anxiety and depression. Anxiety involves fear of potential future problems while depression involves rumination about the past. The focus on what's happening now, produced by mindfulness training, prevents thinking about the past producing depression and thinking about the future producing anxiety.
The findings in the research literature are important as depression causes great distress, impairs functioning, and might even make the person with cancer less able to follow their cancer treatment plan. In addition, high levels of anxiety are stressful, depleting the patient's energy and reducing their ability to fight the cancer or other potential infections. Hence the ability of mindfulness training to reduce the depression and anxiety is important for not only the patients' mental health but also for their physical ability to fight the cancer.
So, reduce anxiety and depression among patients with cancer with mindfulness.
Anxiety and depression are common among patients with cancer, and are often treated with psychological interventions including mindfulness-based therapy.
The aim of the study was to perform a meta-analysis of the effectiveness of mindfulness-based interventions for improving anxiety and depression in patients with cancer.
Medline, the Cochrane Library, EMBASE, and Google Scholar were searched. The randomized controlled trials designed for patients diagnosed with cancer were included. Mindfulness-based interventions were provided.
The outcomes assessed were the changes in anxiety and depression scores from before to after the intervention. The treatment response was determined by calculating the standardized mean difference (SMD) for individual studies and for pooled study results. Subgroup analyses by cancer type, type of therapy, and length of follow-up were performed.
Seven studies, involving 469 participants who received mindfulness-based interventions and 419 participants in a control group, were included in the meta-analysis. Mindfulness-based stress reduction and art therapy were the most common interventions (5/7 studies). All studies reported anxiety and depression scores. The pooled SMD of the change in anxiety significantly favored mindfulness-based therapy over control treatment (−0.75, 95% confidence interval −1.28, −0.22, P = 0.005). Likewise, the pooled SMD of the change in depression also significantly favored mindfulness-based therapy over control (−0.90, 95% confidence interval −1.53, −0.26, P = 0.006). During the length of follow-ups less than 12 weeks, mindfulness-based therapy significantly improved anxiety for follow-up ≤12 weeks after the start of therapy, but not >12 weeks after the start of therapy.
There was a lack of consistency between the studies in the type of mindfulness-based/control intervention implemented. Patients had different forms of cancer. Subgroup analyses included a relatively small number of studies and did not account for factors such as the severity of anxiety and/or depression, the time since diagnosis, and cancer stage.
Mindfulness-based interventions effectively relieved anxiety and depression among patients with cancer. However, additional research is still warranted to determine how long the beneficial effects of mindfulness-based therapy persist.


the jeremy kyle show - All In London Forum - All In London.
does anybody know the contact number for the jeremy kyle show to appear as a guest?
tried clicking on the link you'd sent below before, but not working anymore..
Look how old the thread is.
Surely they have a website?
"Love all. Trust a few. Do wrong to none."


text:
A 20 415-ös számú mellékút egy rövid, öt számjegyű mellékút Pest megyében, Vác közigazgatási területén. Az M2-es autóút 39-es kilométerszelvénye közelében található váci csomópont négy átkötő ágának egyike: az M2-esről vezeti a Budapest felől érkező forgalmat a 2106-os úton kialakított körforgalomba. Lényegében egyenes folytatása onnét a Vácduka felé vezető 21 113-as út.

Teljes hossza az országos közutak térképes nyilvántartását szolgáló kira.gov.hu adatbázisa szerint 269 méter.

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 kira.gov.hu, lekérdezés időpontja: 2019. április 15.

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redpajama_set_name: RedPajamaWikipedia


Conditions shallow were somewhat like what we saw at the Breakwater the previous day. Lots of particulate in the water shallow, but still decent enough viz. Deeper the particulates cleared out to a respectable 30'-40' viz. Several people said it their gauges were reading 46 or so, but I sure didn't feel cold the whole dive. At the start at the dive, we saw an absolutely huge 'dorid' type nudibranch. It had to have been at least a 10" long. Toward the end of the dive, I found two larger Albus' mating. David got some really nice footage of this with his camera. Deco was uneventful, but a bit boring. We ended up doing 130' for 35 minutes w/ 25 minutes of deco.
At the 10 foot stop we could really feel the waves pushing us about and the surface had gotten a quite a bit more sporty. We ditched our gear and got out of there. Lunch was at Siamese Bay.
Sunday was unbelievably calm. Neither Nils nor myself had been to Butterfly house so we thought it would be a perfect day to check it out.
I'd heard that approach wasn't all that much fun. The reality wasn't all that bad, even though the next day my legs were a little sore from hauling doubles up and down the rocky path.
The plan was to drop at the the end the small rocky point and to follow a 210 heading to deeper water.
The entry is through a small, shallow cove with lots of kelp and rocks. We dropped in about 5' of water to go under some of the kelp and to better watch for rocks. Once we were under we made our way out of the cove and hit an interesting, relatively shallow wall where we spent quite a bit of time poking around for slugs. I found a Triopha catalinae just floating mid-water. Nils spotted a nice Okenia rosacea, and I found another on an egg mass shortly thereafter.
It was a pretty cool dive. I'd like to go back and try to explore the deeper areas.
Sue and I had a Fundies class reschedule for the weekend so we decided to do get some Lobos diving done. We had a fish survey/research goal for the dive so we headed to Twin Peak and checked on the 2 Juv Yellow Eye rockfisht that live at the peaks. To our surprise there was a 3rd one in the crack now :-). It's cute... just like 1 inch long.
On the way back we set some survey lines and actually surveyed them on the second dive with the help of Dionna, Mark and HarryB.
It's was pretty cold but it was a lot of fun!
Great Vis! I'd been wanting to improve my mental map of Lobos. Scooters were a great tool to tie together many of the places I've been to as isolated dives, but hadn't placed in relation to eachother. I have a much better feel for the area now!
From the ramp we headed to the hole in the wall for a quick look. From there I attempted a round trip to the lone metridium and back. We deviated a little into the area behind the hole in the wall on the way back and had to back track before finding our way.
Next we jumped E. across the sand channel to Middle Reef, and followed it to the overhang near the end. The plan from this point was to head over to granite point wall. I wanted to find a patch of hydrocoral that Allison showed me last weekend in the granite point area.
We jumped 60 degrees from the overhang at middle reef to granite point wall. Followed the wall left 'til it turned a corner. From there we jumped north across a sand gap to a 2nd wall, and followed it left to the hydrocoral patch! I was very excited to have found it - it is a very pretty corner covered with hydrocoral/anemonies.
By this time I was starting to get cold. We backtracked to middle reef with a slight detour to the left side of the overhang. We stopped for a second to visit the war bonnet.
We cut across to HITW for fun on the way home and ran into Allison, Rob and Jonathan. Some kelp antics ensued.
It was an incredibly beautiful day topside. After staging bottles in the water, we dropped right at the ramp and scooted on out. Within what just seemed a short amount of time we had passed Crossroads and were on Montana. We ended up checking out the north western side and exploring a couple of cracks that run through the face of the reef and looking at the gently waving gorgonians on the face of the reef.
Saw some really nice spanish shawls around 140' of depth. All of a sudden they seem to be everywhere.
Dionna, Susan and I went diving at Flintstone today. The ocean was like a lake with weak S winds. We headed for Flinstone and had a great time diving the pinnacle. We saw many gorgonias gardens, a lot of rock fish, one touchina very large 10inches long, and a very nice cabezon at the top of the reef.
Vis was 40ft at the bottom 15 in the shallow with a cold 44-48degrees water :-0, which felt like 40F after a week of diving in Mexico. For that reason we cut it short at 30min vs 40min that was the plan.
Diving Flintstone is the right way to get back to local diving :-).
We anchored at Deep Hopkins and started preparing our gear when Jim from the Escapade motored by asking how the vis was. He was bringing in a group that had already had one dive and was planning another in the bay. We had only heard that the vis was about 10' so we past that on. After putting all our deco bottles in the water we geared up, dropped over the side, and clipped the 3 bottles on. Vis at the surface was pretty poor and green. No real current to speak of and the waves/surge was light. We dropped into the darkness with Harry leading. After we tied the reel onto the anchor we set off for a 15min out and 15min in dive. While swimming around I spotted a couple of big Triopha catalinae, 1 Acanthodoris hudsoni but the yellow spots were much bigger than what I have seen before, and a few yellow dorids. Vis on the bottom opened out to about 20' so we were happy. The temp definitely dropped however, it was about 48f and you could feel it getting colder as you decended. Harry almost used all his line going out the 15mins. We turned the dive at the 1/2 way point and we switched from our 32 stages when we reached 1500psi. On back gas we made our way back to the anchor, detached the line, and prepared to ascend. Since the bottom was around 78' we did our switch to the 70' bottle at 60' and rotated the 32% stage back and brought up the O2. At 30' we switched back to back gas and at 20' to O2 then made our way up. The anchor line was definitely difficult to see at 20' but the BBQ sandwiches were calling my name so we quickly made our way up, relaxed for a bit on the boat then hauled ourselves back for lunch. Fun fun fun !!!
Anyways after our gear checks were completed we looked down to see that the viz wasn't too bad and decided to drop right at the ramp. Our path took us out past middle reef and then a jump over to Granite Point Wall and skirting the edge of the rocks until we reached our destination.
After waving hello to our greeting party of rockfish, we clipped off and swam around the reef on the western side. Quite a bit of life was on the reef and I ended up seeing several different types of nudibranchs among the Gorgonians. At one point we were shadowed by a large Ling cod who apparently wasn't too happy with divers invading his turf. Several times he would pick a rock in front of us and strike a pose for us. Viz at the site was a decent 30 feet.
On the way back in we picked up a third buddy when a sea lion decided to dive bomb us right before our first deco stop. The Sea lion liked what she saw as she reappeard to keep us company for our 20' stop later in the deco.
Susan and I went diving today on board the Escapade :-).
Our weekends have been pretty busy with teaching so we decided to create a new diving style. It's called 'Weds with Jim and Ed'. The idea is to take some Weds off and go do some fun diving on the Escapade. Today was the pilot program day.
We woke up before the 6:30 alarm due to some very heavy rain splattering on our window. It looked like the Gods didn't want to cooperate with our diving adventure. Nonetheless we decided to get breakfast, feed Naia (our dog) and head for Monterey.
The drive down was slow with heavy rain and a lot of traffic. I kept telling Susan that the clouds and rain were going to disappear any time soon, but was starting to question the decision to dive today myself. The rain fell harder as we continued to drive south.
The moment we drove into the wharf parking lot, the rain stopped and the sky started to open :-). We met with Jim and Ed whom had gotten pretty wet while running from their car to the boat, where all their water proof gear was stored. Without saying much about the improving weather (so as not to jinx it) we headed out to sea.
The usual conversation about where to go started. Jim Capwell said he didn't bother to look for a good dive site as Susan will most likely tell him where to go anyway. We offered that due to the weather we could try to stay in the Bay, but captain's orders were to head out to sea and see what we get. Although his heart was set on diving in the bay, Beto relented and we drove south.
Half way to Point Pi~nos the sky was pretty blue and the swells were only 6 to 7ft so we headed onward to Carmel. The swells were breaking pretty good on shore so we decided to do something in the Outer Yankee Point area away from the breakers.
We settled on Mt. Chamberlain which is a huge structure with a 800ft long east-west wall. We didn't have our scooters with us so we picked a nice shallow spot to put the downline, at about 135ft. We watched the buoy for several minutes and it didn't look like we had any noticeable current. We got all our gear ready and jumped in near the float.
The moment we hit the water we noticed a deceivingly strong current from the South so we starting kicking towards the float and descended while doing a quick check at 20ft. We had to kick quite a lot to stay within visual range of the line, and we really never got to it but it were close enough to use it as target for our descent. At about 110ft we stopped trying to stay on the line and headed for the bottom.
We reached the bottom at about 140ft on the north end of a sand channel that cut the wall perpendicularly. We located the side of the canyon and headed South. The terrain was amazing. There were gorgonians everywhere; it looks like weed fields but full of red little animals. It was a bit dark due to some brown water near the surface but we had a good 20-30ft vis. We slowly moved against the current as we swam in and out of small cracks and arches on the sides of the canyon.
We located a 4 inche juvenile yellow eye rock fish, and we covered our light and were able to get very close to it and had a nice look with the ambient light. The moment we moved one of our lights close, it swam away and hid in a small crack.
After 15min we finally made it to the main east-west wall. We leveled-off at about 190ft and enjoyed the view for several minutes. The wall goes from 120ft down to 220ft and it's a massive structure. We saw a very large china rockfish and very nice vermillion that were swimming around us, as well as a resident ling cod.
After a very short 20min we headed back inside the wall using a parallel canyon and it was a lot of fun to ascend up the narrow crack from 190 to about 150ft. Once we settled at that depth we went for a short drift dive. We saw a rock crab in a crack, plenty of decorator crabs, and a white umbrella crab on a ridge.
The current at 190 was not very strong so we were able to swim out, but as we swam shallower it was much stronger so we just relaxed and enjoyed the drift towards the center of the canyon. We found the lingcod again and some very nice nudibranchs as we moved north. Everything was covered with corynactus and elephant ear sponges :-). What a beautiful area.
As we drifted with the current I remembered there is a shallow spot (80ft) about 300ft away from the wall and for a moment we let the current take us north hoping to see the peak. As we moved north there was a squirrely current coming from opposite directions which we didn't like, so we headed back to the area where we dropped the down-line and looked for a 130ft peak there. After another 20min in the area we headed for the shallow spot and deployed our bag to let the boat know we were about to start drifting with the current.
The decompression was uneventful until we got to about 30ft. At that depth there were tons of moon jellies swimming around. We looked at them for a while, and then noticed a few broken pearl strands of tiny bubbles floating up from depth. Hmmmm. Where could those tiny bubbles have come from? When we finally surfaced to say hello to Capt Jim and his crew, we asked if by chance a whale might have swam under us during our deco hang. Sure enough, a whale had been swimming in our 'line' of drift... and although we didn't see it, we saw evidence of a gray whale friend!
At the surface we found great conditions, sunny sky and moderate swell. We got back on the boat, removed our gear and went whale watching for a couple of hours. There were rumors of a big pod of dolphin somewhere near Point Joe but we were not able to find them. Better luck next time!
All in all it was a grand day out and a perfect way to break up the week.
We had grantious plan of going to remote locations but the weather gods provides us with a different plan. Defiant the gods we still headed out of the bay and venture towards the Yankee Point but half way there we realized it was not going to happen. We check the NW side of Point Lobos looking for some protection from the swells, and after a while we decided to dive Montana pinnacle outsite of Whaler's Cove. Here the weather finally looked like we will be able to get wet.
Montana is a popular site for cooter divers departing from Whalers cove.However, diving it from a boat allows for more time at the site than travelling on the scooters.
the pinnacle. Sue, Karl and myself descended and head to the NW side of the pinnacle. We visited the sand interphase where we found good colonies of sea pens and some big lincond on the sand. Susan also found a couple of brittle start atached to some gorgonias.
a large GPO but we didn't have much luck.
We saw several large lingcods hanging on the East wall, and swell as many decorate crabs.
After about 20min on the deeper part of the pinnacle we headed up shallowe and torwards the norh peak. In that area we found a pair of large dinora albolineat and Clinton point a Bocachio to us.
deco while looking at jellies and their parasites.


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text:
Past Featured Bands
How Merchcore Works
THIS SHIRT WILL REST IN PEACE
Asfirefalls – Boise, ID
From melodic and harmonious piano tracks to hard hitting dominant guitars, AsFireFalls is definitely a band you want to listen to and don't want to miss live. Although the band has been around since 2011, it was re-formed in late 2013 in Boise, Idaho and quickly became a hard-hitting, energetic, unstoppable metal machine and they definitely know how to show it on stage.
Original music is described by fans as Metal or Electronic/Post Hardcore. When AFF is not playing shows or working on new music, the band enjoys hanging out together as friends, but as soon as they step on a stage and stop being four people and become one band, it quickly becomes apparent that their performance is their true outlet and haven.
About Merchcore
Merchcore.com features one band every day with one exclusive shirt to be sold for 24 hours to never be seen again!

redpajama_set_name: RedPajamaCommonCrawl


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Unfortunately it is not that easy to delete old Support Desk messages.
tion flag (status I0076) for every message you do not need any more.
lete the CRM messages anymore using the report.
Will you be able to guide me on how to set the deletion flag (status I0076) for the messages that i do not need anymore.
One more question. If i use this method will the solutions that i have maintained in the solution database will be erased as well?
According to SAP , it is not possble to delete service desk messages since it will lead to inconsistency in the system.


'Abortion' is the term used when someone chooses to go through a medical process to end their pregnancy. It is also known as a 'termination' or a 'termination of pregnancy'.
The decision to have an abortion is an important one. This page will give you information on the choices you have and what is involved.
an abortion is not the same as a miscarriage, which is where the pregnancy ends without medical intervention.
about a third of women in the UK will have an abortion by the time they are 45.
If you are in a relationship, you might want to talk to your partner about your options. You may want to consider your circumstances together. But even if your partner is the person who you became pregnant with, that doesn't mean they can tell you what decision to make. It's your body and your choice.
Abortion is a very safe procedure when carried out under the care of a hospital or licensed clinic. The earlier an abortion is carried out, the safer it is.
Lots of studies have found that abortion is unlikely to affect your chances of becoming pregnant in future.
you may find it helpful to talk to someone you trust - this might be a friend or family member, or a health professional or support worker. There are lots of organisations that can give you informed, confidential advice and support (see below).
you should seek immediate medical help after having an abortion if you experience heavy bleeding, severe pain or have a high temperature.
Women on web questions and answers on abortion.
If you want to have an abortion through the NHS, you'll usually need to be referred to a specialist service that deals with abortion. You can ask your GP to refer you or you can go to your local sexual and reproductive health clinic. Find your nearest clinic.
In some areas of England you can refer yourself. To find out if you can self-refer for NHS-funded abortion care, call Marie Stopes on 0345 300 8090 (24 hours).
Generally, an abortion should be carried out as early in the pregnancy as possible, usually before 12 weeks and ideally before 9 weeks where possible.
In the UK, it is legal to have an abortion up to 24 weeks. If there is a serious health risk to a mother or baby, this can be extended.
During your first appointment with your GP or clinician, you should be given the opportunity to talk about your situation and which method of abortion is suitable for your stage of pregnancy. You will also be advised about any related risks and complications.
You should also be tested for sexually transmitted infections (STIs).
You may also be given information and advice about your contraceptive choices after the abortion.
complete a finger prick blood test to check which blood group you are.
You will be asked to sign a consent form before the abortion is carried out.
There are several different procedures, depending on what stage of pregnancy you have reached, read about those here.
How is the 'how many weeks pregnant' calculated?
From the first day of your last period. For women with a menstrual cycle of average length, that day is usually about two weeks before conception, which explains why pregnancies are said to last 40 weeks.
Will I be able to see the embryo during an early stage abortion (up to 9 weeks)?
Depending on the length of the pregnancy, a small pregnancy sac may be visible.
Most of the time women can see blood and tissue in their sanitary pad or in the toilet. The (very small) embryo is usually passed within this blood and tissue, and so often goes unnoticed.
At 8-9 weeks pregnant, you might see a sac in the blood and it may be possible to see the embryo (at this stage the embryo is about 2.5 cm) this can be distressing. It is best to flush the toilet and throw sanitary pads away as usual.
An early stage abortion is possible without an internal procedure. Three clinic appointments are needed.
You will be asked to take two different medicines 36-48 hours apart.
On your first visit you will be given an abortion pill to block the hormone that makes the lining of the womb suitable for the fertilised egg. A few women will have mild cramps and a little bleeding at this stage. If you have heavy bleeding or significant pain, you should contact the hospital or clinic where you had your appointment. If it is out-of-hours, you should go to the nearest A&E department.
Two days later, you will be given the second medicine and within four to six hours, your womb will expel the pregnancy. This part of the process can be painful, but you can take a painkiller. Bleeding may be heavy and you may notice some clotting. Some women can find this distressing so you should talk to your clinician in advance about what to expect.
Some services have facilities for this process to happen at the clinic. Some women prefer to go through this process at home. This can be your choice.
This method uses gentle suction to remove the foetus from the womb. The procedure usually takes 5 to 10 minutes and can be carried out in a day surgery unit under either sedation local anaesthetic or general anaesthetic. If you have sedation or general anaesthetic you will need to have someone with you after the procedure and you will not be able to drive home yourself.
You will usually be able to go home the same day.
a small, plastic suction tube connected to a pump will then be inserted into your womb and used to remove the foetus and surrounding tissue.
Following the procedure, you will usually experience vaginal bleeding for up to 21 days.
In most cases, the bleeding will be quite heavy for two to three days before settling down. You may experience cramps for which you can take painkillers at home.
This is a surgical procedure carried out under general anaesthetic. It usually takes 10-20 minutes and, if you are healthy and there are no complications, you may be able to return home the same day.
Your cervix will be gently stretched and dilated. Forceps and a suction tube will be used to remove the foetus and tissue within the womb.
You may experience vaginal bleeding for up to 21 days afterwards.
This method uses the same medication as an early medical abortion but will take longer and more than one dose of medication may be needed.
You will usually be able to return home on the same day. However, sometimes an overnight stay in hospital may be required.
In a small number of cases (less than 1 in 20), the placenta or afterbirth does not pass. In this case, you may need to have a small operation under a general anaesthetic to remove the placenta.
There are two options and both require an overnight stay in hospital and are carried out under general anaesthetic.
stage two (carried out the next day) involves removing the foetus and surrounding tissue.
your cervix will be gently stretched and dilated and forceps and a suction tube will be used to remove the foetus and tissue within the womb.
All of these procedures can feel invasive and distressing, so you may want to consider having someone nearby to support you when you leave hospital. It's also important to talk to your clinician about what to expect during and after any procedure.
What is and an ultrasound scan?
An ultrasound scan uses high frequency sound waves to create an image of part of the inside of the body. Ultrasound scans can give a more accurate estimation of the length of the pregnancy than counting the weeks from the last period.
What does the law say about abortion in the UK?
The Abortion Act 1967 covers England, Scotland and Wales but not Northern Ireland.
there is substantial risk that the child would be born with serious physical or mental disabilities.
How would I know if I was going to have a baby with a high chance of serious genetic or physical abnormality?
This is usually picked up during the routine ultrasound screening tests that are part of antenatal care.
Does an abortion go on my health record?
Yes, the abortion will usually go on your health record if you have been referred by your GP. If you self refer, the clinic may encourage you to allow them to inform your GP but this is not automatically done as it is a confidential service.
Does my fertility reduce after having an abortion?
No, your fertility should return to normal after an uncomplicated abortion. In fact, women can be experienced an increase in their fertility soon after a termination so it is important to start contraception straight away to prevent another pregnancy from happening.


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A Bibliophile's Reverie
Biblophile: A cocktail of unparalleled un-orthodoxy and intoxicating imagination
Steampunk Month:Interview with Becket & Review of Becket's Key The Steampunk Vampire Girl and the Dungeon of Despair
Posted by fantastyfreak on March 14, 2015 March 14, 2015
Amazon (Kindle)/ Amazon (Paperback)/Barnes & Nobles/Kobo/Goodreads
Fitting with both the Steampunk aesthetic of the book, along with the prevailing mood of the story, here is a techno-violin piece from independent musician/writer Emilie Autumn
With the deftness of a skilled storyteller, Key the Steampunk Vampire Girl excels in having much to say, while being written first and foremost as a deeply entertaining fantasy tale, geared for readers of all ages. The story utilizes subtle writing, to carefully sculpt the feelings of all the colorful main characters and side characters that populate the creative, imaginative world that Becket skillfully molds in this story.
Separated from her parents and the warm, nourishing household they provided, the main character of this story Key the Steampunk Vampire Girl (an ordinary girl, before being turned into a vampire) is thrown into a world of literal despair, or the underground kingdom, where the vampires of this world live. The emotional travails of the main character Key is poignant and sensitively-expressed, and there is an ultimate cathartic arc for Key's growth as a character, proving the skill of Becket as a storyteller to provide meaning and emotional depth to the story. Conversely, There is no comfort here unlike her family's abode, and readers that may have faced feelings of alienation, separation from somewhere where they are loved unconditionally at some point in their livers will greatly empathize with Key's characters at this stage, and learn about the deeper magic of strength-building tools we all have in our spiritual arsenal like perseverance.
Enhancing the writing of story are beautiful, detailed drawings by artist Raven Quinn, whose awesome Steampunk story art bring the crucial moments of Key's tale to life. The drawings never detract from the dramatic flow of the story, rather they benefit the story by really enlivening the feel and texture of the story, giving it a powerfully surreal element that really adds to the dramatic tone that the story does a great job at delivering to the reading.
Also, there are moments of levity and downright brilliant wordplay that linguist nerds, like myself, will enjoy. Different names of places and of characters have a Dickensian feel to them, as the names themselves offer both a satirical and facetious dimension to the many vibrant places or eccentric characters that are a large element of this story's success as a richly-layered, entertaining tale.
Above all, the story has the mystifying, edifying magic of a true allegory, in that the story has both philosophical and ethical depth that will most definitely have a strong, sobering effect on the reader, forcing them to reflect on the different emotions they've felt during rough, taxing moments of their lives, and to be reminded of the deeper magic that lays buried within the deepest parts of our minds or lives, waiting to be discovered and work its healing magic on the reader. Becket is truly a skilled wordsmith, whose stories deserve to be read and enjoyed by readers of all ages. Key: the Steampunk Vampire Girl: Dungeon of the Despair comes with a very high recommendation from me. I cannot wait to start the sequel, and become once again transported to the vibrant, complex Steampunk world where Key and others reside.
Interview with Becket: (Author of the Key the Steampunk Vampire Books, and Assistant for Writer Anne Rice)
1. BR:When did you first discover the Steampunk genre, and what do you think explains its popular appeal among so many people, especially within the next decade or so?
Becket: I think that the steampunk subculture has been building up for many decades now. I first noticed it watching reruns of the old television show The Wild Wild West when I was perhaps ten years old. I remember being incredibly influenced by all the gadgets used by James West and Artemus Gordon; and I even tried to make some myself and take them to school, but teachers confiscated them. I did not know that I had a love for steampunk at that time, because the term was probably coined around then, and I was too young to know it. But my love for it was further fueled by reading H. G. Wells and Mary Shelley, and other authors who basically wrote Victorian science fiction. It makes me wonder if readers centuries from now will look on our contemporary science fiction and consider it to be their own version of steampunk. For me, I see steampunk as a blending of Gothic romance and Victorian science fiction; and I believe that people are so attracted to it because it uniquely combines the rules and regulations of Victorian romance and science fiction, it also allows the reader to conceive of greater possibilities within those rules and regulations.
Property of Raven Quinn (artist of this work) & Becket
2. BR:What inspired the idea behind the novel, Key the Steampunk Vampire Girl?
Becket: I had always wanted to write a vampire story, ever since I first read Anne's novels in my early teens. I fell in love with the vampire literature at that time, reading anything from Bram Stoker's Dracula to Michael Romkey's I, Vampire. Back then had a dearth of quality vampire literature; and I recall how I lamented that fact, as I strolled through the local Barnes & Noble, which was still a novelty then. Also, I have always been a fan of books for middle graders, such as books by Roald Dahl, Lewis Carroll, or Norton Juster. Because I love the lightheartedness and imagination of that particular genre, I wanted to infuse it with my love for vampire literature and the steampunk subculture. The title of the book actually came to me after I watched an interview with Joss Whedon, explaining how he came up with the title Buffy the Vampire Slayer. He said that each word in the title was specific to describing the character. She was a slayer, so she had power; there were vampires, so there was danger; and her name was Buffy, which infused the character with that Whedonesque humor many of us grew up with, since the name Buffy was synonymous with being an airhead. Key the Steampunk Vampire Girl followed suit. She is a Girl to show her vulnerability; she is a Vampire to show her ferocity; she is Steampunk to illustrate the propensity of her taste; and her name is Key to show that she herself is the key to the answers in her own life.
3. BR:As the story is geared for the Middle-Grade Crowd (though appeals to a very wide audience), did you intentionally, or unintentionally set out to include a powerful allegorical core that is really important to the emotional effectiveness of the story?
Becket:First off, thank you for calling Key's allegorical core powerful. I want everything I write to be meaningful, so I always set out to write a story that has heart, humor, and an important message. Sometimes, when I'm writing the first draft, in the same way that I have a vague idea of the whole narrative, I also vaguely see the potential it has to be a powerful allegory. But it is usually after the third draft that I clearly see the power my story has intrinsically. Once I see that, the plots in all subsequent drafts are shaped around it, but at that point there usually is not too much drastic shaping because the plots and the allegorical power are pretty tightly woven together by then.
4. BR: Do any more adventures lie on the horizon for Key the Steampunk Vampire Girl?
Becket: As a matter of fact, Key's next adventure is titled KEY THE STEAMPUNK VAMPIRE GIRL AND THE FLOATING MANSION (due April 7, 2015). In this third installment of her adventures, she reunites with many of her old friends, including Mr. Fuddlebee, Miss Broomble, Tudwal the immortal puppy-wolf; and she also meets several other characters such as Tedric Tinker and the Tinker Family, as well as ARTH-R (A Really Thoughtful Help-Robot). Also, from Good the Goblin Queen, there is even a crossover character: The Clock Goblin makes an appearance toward the end to help save the day! This is one of my most favorite adventures of Key the Steampunk Vampire Girl.
5. BR:Is there anything else you wish to say for readers of A Bibliophile's Reverie, who may be interested in reading this or your many other top-notch books?
Becket:If you enjoyed the first six installments of the Blood Vivicanti, several more will be coming in the fall this year, 2015. Mary Paige has a whole new adventure before her, and there are more steampunk elements in these new episodes of her story, as we see just how old the Blood Vivicanti can grow, just how powerful she can be, and just how wonderfully the whole world can evolve in the coming centuries!
Thanks so much Becket for your thoughtful,interesting answers for my questions, and for sharing your feelings on the steampunk genre/the development of Key the Steampunk Vampire Girl with your many fans that read this blog!!
If you are interested in learning more about Becket's published works, be sure to visit either his Facebook Fan Page, or website
Be sure to checkout a review of Key the Steampunk Vampire Girl and the Tower Tomb of Time next week!
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text:
use std::ops::BitOr;

#[allow(unused)]
use crate::Arg;
#[allow(unused)]
use crate::Command;

// Third party
use bitflags::bitflags;

#[doc(hidden)]
#[derive(Debug, Copy, Clone, PartialEq, Eq)]
pub(crate) struct AppFlags(Flags);

impl Default for AppFlags {
    fn default() -> Self {
        AppFlags(Flags::COLOR_AUTO)
    }
}

/// Application level settings, which affect how [`Command`] operates
///
/// **NOTE:** When these settings are used, they apply only to current command, and are *not*
/// propagated down or up through child or parent subcommands
///
/// [`Command`]: crate::Command
#[derive(Debug, PartialEq, Copy, Clone)]
#[non_exhaustive]
pub(crate) enum AppSettings {
    IgnoreErrors,
    AllowHyphenValues,
    AllowNegativeNumbers,
    AllArgsOverrideSelf,
    AllowMissingPositional,
    TrailingVarArg,
    DontDelimitTrailingValues,
    InferLongArgs,
    InferSubcommands,
    SubcommandRequired,
    AllowExternalSubcommands,
    Multicall,
    SubcommandsNegateReqs,
    ArgsNegateSubcommands,
    SubcommandPrecedenceOverArg,
    ArgRequiredElseHelp,
    NextLineHelp,
    DisableColoredHelp,
    DisableHelpFlag,
    DisableHelpSubcommand,
    DisableVersionFlag,
    PropagateVersion,
    Hidden,
    HidePossibleValues,
    HelpExpected,
    NoBinaryName,
    #[allow(dead_code)]
    ColorAuto,
    ColorAlways,
    ColorNever,
    Built,
    BinNameBuilt,
}

bitflags! {
    struct Flags: u64 {
        const SC_NEGATE_REQS                 = 1;
        const SC_REQUIRED                    = 1 << 1;
        const ARG_REQUIRED_ELSE_HELP         = 1 << 2;
        const PROPAGATE_VERSION              = 1 << 3;
        const DISABLE_VERSION_FOR_SC         = 1 << 4;
        const WAIT_ON_ERROR                  = 1 << 6;
        const DISABLE_VERSION_FLAG           = 1 << 10;
        const HIDDEN                         = 1 << 11;
        const TRAILING_VARARG                = 1 << 12;
        const NO_BIN_NAME                    = 1 << 13;
        const ALLOW_UNK_SC                   = 1 << 14;
        const LEADING_HYPHEN                 = 1 << 16;
        const NO_POS_VALUES                  = 1 << 17;
        const NEXT_LINE_HELP                 = 1 << 18;
        const DISABLE_COLORED_HELP           = 1 << 20;
        const COLOR_ALWAYS                   = 1 << 21;
        const COLOR_AUTO                     = 1 << 22;
        const COLOR_NEVER                    = 1 << 23;
        const DONT_DELIM_TRAIL               = 1 << 24;
        const ALLOW_NEG_NUMS                 = 1 << 25;
        const DISABLE_HELP_SC                = 1 << 27;
        const ARGS_NEGATE_SCS                = 1 << 29;
        const PROPAGATE_VALS_DOWN            = 1 << 30;
        const ALLOW_MISSING_POS              = 1 << 31;
        const TRAILING_VALUES                = 1 << 32;
        const BUILT                          = 1 << 33;
        const BIN_NAME_BUILT                 = 1 << 34;
        const VALID_ARG_FOUND                = 1 << 35;
        const INFER_SUBCOMMANDS              = 1 << 36;
        const CONTAINS_LAST                  = 1 << 37;
        const ARGS_OVERRIDE_SELF             = 1 << 38;
        const HELP_REQUIRED                  = 1 << 39;
        const SUBCOMMAND_PRECEDENCE_OVER_ARG = 1 << 40;
        const DISABLE_HELP_FLAG              = 1 << 41;
        const INFER_LONG_ARGS                = 1 << 43;
        const IGNORE_ERRORS                  = 1 << 44;
        const MULTICALL                      = 1 << 45;
        const EXPAND_HELP_SUBCOMMAND_TREES   = 1 << 46;
        const NO_OP                          = 0;
    }
}

impl_settings! { AppSettings, AppFlags,
    ArgRequiredElseHelp
        => Flags::ARG_REQUIRED_ELSE_HELP,
    SubcommandPrecedenceOverArg
        => Flags::SUBCOMMAND_PRECEDENCE_OVER_ARG,
    ArgsNegateSubcommands
        => Flags::ARGS_NEGATE_SCS,
    AllowExternalSubcommands
        => Flags::ALLOW_UNK_SC,
    AllowHyphenValues
        => Flags::LEADING_HYPHEN,
    AllowNegativeNumbers
        => Flags::ALLOW_NEG_NUMS,
    AllowMissingPositional
        => Flags::ALLOW_MISSING_POS,
    ColorAlways
        => Flags::COLOR_ALWAYS,
    ColorAuto
        => Flags::COLOR_AUTO,
    ColorNever
        => Flags::COLOR_NEVER,
    DontDelimitTrailingValues
        => Flags::DONT_DELIM_TRAIL,
    DisableColoredHelp
        => Flags::DISABLE_COLORED_HELP,
    DisableHelpSubcommand
        => Flags::DISABLE_HELP_SC,
    DisableHelpFlag
        => Flags::DISABLE_HELP_FLAG,
    DisableVersionFlag
        => Flags::DISABLE_VERSION_FLAG,
    PropagateVersion
        => Flags::PROPAGATE_VERSION,
    HidePossibleValues
        => Flags::NO_POS_VALUES,
    HelpExpected
        => Flags::HELP_REQUIRED,
    Hidden
        => Flags::HIDDEN,
    Multicall
        => Flags::MULTICALL,
    NoBinaryName
        => Flags::NO_BIN_NAME,
    SubcommandsNegateReqs
        => Flags::SC_NEGATE_REQS,
    SubcommandRequired
        => Flags::SC_REQUIRED,
    TrailingVarArg
        => Flags::TRAILING_VARARG,
    NextLineHelp
        => Flags::NEXT_LINE_HELP,
    IgnoreErrors
        => Flags::IGNORE_ERRORS,
    Built
        => Flags::BUILT,
    BinNameBuilt
        => Flags::BIN_NAME_BUILT,
    InferSubcommands
        => Flags::INFER_SUBCOMMANDS,
    AllArgsOverrideSelf
        => Flags::ARGS_OVERRIDE_SELF,
    InferLongArgs
        => Flags::INFER_LONG_ARGS
}


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While a contributor day in the core team gets you familiar with the technical parts of contributing to WordPress core, it usually does not provide further guidance for those who are interested in following up and contributing more than the first taster. Finding your spot among the core contributors and spending your contributing time efficiently can be a tough and tedious challenge. Furthermore, you may run into roadblocks or get frustrated quickly.
If you are interested in becoming a long-term contributor and influencing WordPress core sustainably, this talk is for you – even if you haven't attended a contributor day before. The session will be a personal story that highlights benefits of contributing, provides recommendations on how to find your spot and build trust, and gives insights on which traits are particularly valuable to have or pick up. You will furthermore learn how contributing to core is by far not only about writing code. The goal of this session is to prepare you for a beneficial long-term relationship as a core contributor so that you can make a significant impact over time.


text:
Studying breast cancer on a genomic scale using DNA microarrays
D Botstein1 &
PO Brown1
Genome-wide studies of human gene expression have become possible in recent years because of the availability of most of the sequences of protein-encoding genes. Technology has been developed in our group to obtain and analyze patterns of expression of many thousands of genes at once. We have begun to apply cDNA microarray technology to the study of cancer, focusing on breast cancer. We characterized the variation in patterns of gene expression in a set of 62 surgical specimens of human breast tumors from 40 different patients, using cDNA microarrays representing 8102 different human genes. The observed gene expression patterns provided a remarkably distinctive molecular portrait of each tumor. Twenty of the tumors had been sampled twice, both before and after a 16-week course of doxorubicin chemotherapy, and two tumors were paired with a lymph node metastasis from the same patient. The gene expression patterns observed in the serial samples from the same tumor, and the tumor/metastasis pairs, were almost always more similar to each other than either was to any other samples. Clusters of co-expressed genes were identified, for which variation in mRNA levels could be related to specific features of physiological variation, or to variation in the cellular constituents of the tumors. The tumors could be classified into subtypes that were distinguished by pervasive differences in their gene expression patterns. Such classifications were robust, and reflected changes in gene expression in the epithelial cells as well as differences in expression derived from the populations of non-epithelial cells in the tumors. These results suggest that gene expression patterns can provide distinctive and recognizable molecular portraits of individual tumors, and perhaps a basis for a new molecular classification of cancers.
Stanford University School of Medicine, Stanford, California, USA
D Botstein & PO Brown
D Botstein
PO Brown
Botstein, D., Brown, P. Studying breast cancer on a genomic scale using DNA microarrays. Breast Cancer Res 2, S.36 (2000). https://doi.org/10.1186/bcr196
Gene Expression Pattern
Human Gene
cDNA Microarray


Koga's Koffing - Pokémon Planta Comum - 79/132 - Gym Challenge!
Smelly Gas - Flip a coin. If heads, this attack does 10 damage to each Benched Pokémon (including your own). (Don't apply Weakness and Resistance for Benched Pokémon).


Equal Housing Opportunity– What everyone should know about equal opportunity in housing.
The sale and purchase of a home is one of the most significant events that any person will experience in his lifetime. It is more than the simple purchase of housing, for it includes the hopes, dreams, aspirations and economic destiny of those involved in it. Read exactly what Equal Housing means to you as a buyer or seller of real estate here.
REALTORS® are subject to disciplinary action and sanctions if they violate the duties imposed by the Code of Ethics. The Code of Ethics is a detailed document that spells out the professional responsibilities of every REALTOR®. Click below to read the 2016 REALTOR® Code of Ethics in it's entirety.
At Home With Diversity® is an educational experience designed to present a picture of the changing face of the real estate industry. Since 1998, the At Home With Diversity® program has prepared over 20,000 REALTORS® to work effectively with and within today's increasingly diverse pool of homebuyers.


The colposcopic appearance of the cervix during pregnancy depends on gestational age. During pregnancy, the cervix enlarges, becomes soft and congested, and is covered with copious mucus. The columnar epithelium becomes everted and hypertrophied. The SCJ is easily visualized. During the second and third trimesters, the stroma of the cervix may undergo focal decidual change, which appears as a raised whitish plaque or as a pseudopolyp. The blood vessels on the cervix are prominent during pregnancy.
In advanced pregnancy, the cervix becomes so hypertrophied and soft that performing colposcopy satisfactorily becomes difficult. Even metaplastic changes and low-grade lesions look dense white after application of acetic acid. As a result, there may be over diagnosis of low-grade abnormalities.
The goal of colposcopy during pregnancy is to rule out invasive cancer. Biopsies should be obtained only if invasive cancer is suspected. Biopsy of suspected precancerous lesions or treatment of precancerous lesions should be contemplated 6–12 weeks after delivery, when colposcopy can be repeated.


If I were to believe my daughter, earrings are like handbags, a lady can never have enough. On this page you will find studs, dangles, hoops, huggies drop and earring jackets. So many different styles, all with beauty and value.
I do not specialize in solitaire diamonds, but I do have a small selection of diamond studs available (on the diamond earrings page) which are all at extraordinary prices. Again, if you believe my daughter, no earring collection is complete without a pair of diamond studs.
A wonderful pair of vintage earrings, full of character and uniqueness.
Lovely pair of dangle earrings with some millgrain work to give them a classic feel. 1.37 carat of lively sphene's with a halo of diamonds.
14k Stamped Yellow Gold Blue Sapphire Diamond Earrings.
High-end blue sapphire approximately .6 carat each with a halo of 10 dazzling diamonds of approximately .3 carat each. Come with secure silicone and 14k gold backs.
As classic and as classy as it gets.
Stamped 14k Yellow Gold Black Pearl and Diamond Earrings.
Cute dangle black pearl earrings with 5 diamonds in each earring. Pearls are 6.5mm.
Stamped 18 Yellow Gold Sapphire, Ruby, Emerald and Diamond Earrings.
Unique earrings in the shape of a ball. High quality gemstones and diamonds make these earrings very desirable.
14k Stamped Yellow Gold Multi-Gem Diamond Earrings.
Delightful huggie style blue sapphire, ruby, emerald and diamond earrings.
Lovely quality and classic elegance.
14 Yellow Gold Sphere Earrings.
Seen here with matching ring Item No AB7 USD445. The set can be purchased together for USD1,100.
High quality earrings with 5 carat of garnet and .33 carat of sparkling diamonds in each earring.
WOW ! Custom made with super high quality materials. Approximately 1.55 pink sapphire in each earrings along with approximately .25 carat of high quality diamonds and 11.6mm perfectly round and blemish free south sea pearls, all set in 18K white gold.
Supremely elegant and substantial earrings by "Rosato" with .29 very high quality diamonds.
Came with the original price tag of $3,360.
Exquisitely made by New York artisans, these delightful earrings have .24 carat of lovely bright diamonds.
Enamel is never inexpensive, except at Trillion Jewels of course.


Most people these days don't have nearly enough time to go outdoors and fill their lungs with some fresh air, for a change. If you happen to have some spare days and you're looking to spend some quality time with your family, it might be a good idea to organize a camping trip.
It's very tempting to leave the kids playing video games on the weekends because they keep them busy and happy. But let's face it. Sitting in front of the computer or cooped up in the house all day long is anything but healthy, especially when the world outside has so much to offer.
We made a list of fun activities you can organize for your next camping trip. Have a look at them below.
Board games are fun, and that's why they've become so popular over time. They engage everyone, depending on the number of players allowed by the rules or the pieces. But if you don't have the convenience of a board game, worry not.
You can play charades, or I Spy, or even Hide & Seek, but make sure that the area you camp in is safe from dangers. The last thing you'd want to do is have the whole family look for one of your kids.
If nothing else crosses your mind, you can even do with some paper and a pencil. You'll be able to play Dots, Hangman, or Tic-Tac-Toe. It's that easy — so you just have to use your imagination to make do with all of the basic resources you might have.
According to some sources, one in eight British individuals has never seen a real cow in their life. Don't you think that's tragic? Even if none of your family members or yourself want to become a farmer one day, exploring nature should be an important part of your lives. You can watch animals, birds, collect bugs, gather flowers, pine cones, and rocks, and even leaves for your collection.
If you are passionate about photography, you can take really good nature pictures.
So maybe not everyone is passionate about music, in general, but surely all of us have hummed to a song in the car or while listening to the radio when running or cleaning the house. It doesn't take that much to learn how to play an instrument, and one of the easiest ones is the ukulele. You can even go for an acoustic guitar and play some of the best-known blues and country songs ever to have been written.
Think about it. How cozy would it be for you and your friends and family to gather around a campfire and sing some songs?
Just make sure that you take all the safety precautions so that fire doesn't get out of control. We all know how camping might involve beer drinking, so it's important for at least one person to remain sober and ensure that everything is okay before everyone goes to sleep.


Baby girls abound at Rustici Software right now. Mike's daughter Amelia was born on October 11th and my daughter Lindy was born on October 8th. Both girls were perfectly healthy and are doing great.
Please be patient with us as we adjust to the new schedules.


text:
Q&A Sep 1, 2021
Q&A: Talking with Toyin Ogunfolaju, Infrastructure and Social-Economic Inclusion Leader
Toyin Ogunfolaju, Infrastructure and Social-Economic Inclusion Leader at Jacobs, explains what social-economic inclusion (SEI) means, how to understand and engage a community, and why SEI is integral to Jacobs' work.
Communities demand to be understood – to have their thoughts, perspectives, and history incorporated long before an infrastructure project breaks ground. However, social-economic inclusion is not just a box to check, but a process integral to work that benefits the communities we work in and creating a more equitable world.
We talked with Toyin Ogunfolaju, Infrastructure and Social-Economic Inclusion (SEI) Lead at Jacobs, to learn more.
Can you explain your role with Jacobs?
I currently serve as a program/project manager for several infrastructure projects throughout the mid-Atlantic and Northeast regions of the U.S.
Within Jacobs, I serve as the Social-Economic Inclusion (SEI) Lead, for the North, including Canada, Midwest, Northeast and Mid-Atlantic regions. In that role, I partner with sales, project delivery, even internal operations leaders to make sure that as the company pursues and delivers work, we're maximizing social economic value.
How would you define equity and inclusion in the context of infrastructure and transportation?
I define equity in infrastructure and transportation as making sure systems are set up in a way so that everyone is working from the same baseline. For example, so that financial systems are not partial to certain societal groups, or it could be how we expose, educate, recruit & retain, hire and promote our workforce.
Then, once everyone is on the same baseline (equity), inclusion is about making sure there's a seat at the table – the decision-making table, the influence table – giving them voice and room so that different perspectives are heard.
Why is it important for clients to consider equity and inclusion in this space?
Social economic inclusion in the infrastructure space is not mutually exclusive with delivering infrastructure projects. You don't build or run a transportation system in a silo. Equity and inclusion are not just a fad, a feel-good gesture or a nice-to-have. Communities and stakeholders are demanding this understanding and incorporation of their thoughts, perspectives, background and history into why we move forward the way we do in infrastructure. Eventually, there won't be a need for a subcommittee on it – the question will be "why wasn't this system equitable or inclusive before?"
How do we approach, for example, transit planning through an equity lens?
We think about it from a quantitative (data) and a qualitative (humanistic) standpoint. There's so much information out there that we're collecting around who's doing what, where and how, especially in transportation – ridership, vehicle miles driven, whether you take a train or a plane, why a community is more likely to use a certain system. There's a pattern and a narrative that data is telling us. We really need to connect the dots to discover that story which a community may not necessarily verbalize. On the humanistic side – because after all, it's about the humans using these systems – it's about optimizing community-based, community-led design on infrastructure projects.
What are some other/different ways to engage communities and increase the positive impact a project can have on a community?
To bring stakeholders to the table, you have to plan ahead of time. You don't break ground on a project and then suddenly think about how to be inclusive – it's about early intervention. It's often difficult to bring the community to the table. Why? Because people work! They're focused on their families, getting to work, and keeping a roof over their heads – they don't always have time to fill up the community meetings and read about your projects. It's about knowing your community well enough to engage with them proactively and in ways convenient to them. For example, we may know that in a given community, many people go to farmers markets on Saturday. So, we're figuring out ways to approach and engage the community in a more organic (no pun intended) way – they're already there. Once safe during the pandemic, block parties are another great opportunity to engage with folks. It's also about maximizing the A for Arts in in STEAM – thinking about more ways to include diverse perspective on a traditionally more technical field and make this conversation for everyone.
How is Jacobs thinking differently about the way it engages with clients and communities on these issues?
One of the great partnerships that Jacobs has formed is with Simetrica, a U.K. based company, which brings a lot of our expertise around social value. Social value is the monetization of the outcomes that we see when a certain policy is put in place or a program invested in. This tool allows us to develop robust measurements around the actual social value or impact you're having on society and stakeholders with your project – and its entirely data-driven.
What norms do you hope to challenge in this space in the future?
I want to dispel this idea that SEI is just a fad and we'll look back at this time and remember it as such. One of the sub-areas of my SEI role is to help Jacobs better partner with small, diverse firms – not just to check the box, but to really partner with them in more meaningful ways. For example, when we partner with other firms on projects, we're offering a more significant mentor and protégé relationship that folks can really sink their teeth into and build their capabilities around.
We're not focusing on this just because clients require it, but because we understand that this is how we'll grow our business. I think that someday, firms like Jacobs will still acquire technical firms but also will be acquiring smaller, community-based firms and organizations who know the local geography and stakeholders, who look like and understand their local communities.
Why is it important to stay curious?
I've always been intellectually curious and asked questions. You don't know what you don't know. If you're not asking questions, you can't identify gaps. You can't identify a perspective that maybe you hadn't considered. A true conversation and public discourse, and the best way to learn, is to question something. In inclusion, this means if someone is coming to the table with a perspective that I have not considered or factored into my decision, you ask, "why didn't I include that?" Being curious means you're willing to acknowledge that you don't know everything, and you may not always get it right.
What is the most fulfilling part of your career?
After I had my son, I realized that I wanted to work in a role and at a company in which my work would impact the folks that I engage with every day in my community. So, the most fulfilling thing about my career today is that every year, and every new role or responsibility I take on, helps me better show how dynamic people are, what people bring to the table, and that we're all human. We all have similar baseline desires – to love and be loved, to be happy, to belong and be accepted and understood – I get to work every day on projects to help people feel understood. I get to work with awesome, smart people who care. I'm at the point in my career where I get to really impact people and work for a company that provides me the resources to be able to do that.
What drives you drives us as we work to build a better world – together. At Jacobs, every day is an opportunity to make the world better, more connected, more sustainable.
We're always looking for dynamic and engaged people to join our team. Bring your passion, your ingenuity and your vision. Let's see the impact we can create, together.


text:
Home » Brentwood- PEN/Bellwether Prize Winner Ron Childress discusses and signs "And West is West"
Brentwood- PEN/Bellwether Prize Winner Ron Childress discusses and signs "And West is West"
DIESEL, A Bookstore in Brentwood welcomes 2014 PEN/Bellwether Prize winner Ron Childress to the store to discuss and sign his debut novel, And West is West, on Thursday, October 8th at 6:30pm. Joining Ron in conversation will be the 2008 PEN/Bellwether Prize winner, Heidi W. Durrow.
The Pen/Bellwether Prize, which was established in 2000 by Barbara Kingsolver and is funded entirely by her, was created to promote fiction that addresses issues of social justice and the impact of culture and politics on human relationships. The prize is awarded biennially to the author of a previously unpublished novel of high literary caliber that exemplifies the prize's founding principles.
And West Is West is an inspired novel about the devastating power of new technology to corrupt innocent lives. When Jessica, a young Air Force drone pilot in Nevada, is tasked with launching a missile against a suspected terrorist halfway across the world, she realizes that though women and children are in the crosshairs of her screen, she has no choice but to follow orders. Ethan, a young Wall Street quant, is involved in a more bloodless connection to war when he develops an algorithm that enables his company's clients to profit by exploiting the international instability caused by antiterrorist strikes. Though only minor players, the actions of these two people have global ramifications that tear lives apart including their own. When Jessica finds herself discharged from the service, and Ethan makes an error that costs him his job, both are set adrift, cast out by a corrupt system and forced to take the blame for decisions they did not make. Childress has crafted a terrifyingly real scenario in which characters navigating different worlds are bound together by forces beyond their control.
Ron Childress started his work life in boatyards up and down the New England coast, but at nineteen, he enrolled in community college and went on to earn his BA, MA, and PhD in literature. Childress worked for several years as a communications manager for a professional association near Washington, DC, before joining his wife in her tech marketing agency. In 2000, he left the business to pursue fiction writing full time. And West Is West, winner of the 2014 PEN/Bellwether Prize for Socially Engaged Fiction, is his first novel.
Heidi W. Durrow is the New York Times bestselling author of The Girl Who Fell From the Sky and 2008 recipient of the PEN/Bellwether Prize. She is a graduate of Stanford, Columbia's Graduate School of Journalism and Yale Law School.
Originally from Portland, Oregon, Heidi has worked as a corporate litigator and as a Life Skills trainer to professional athletes of the National Football League and National Basketball Association. Durrow's writing has appeared in the New York Times, Ebony Magazine, Alaska Quarterly Review, The Literary Review, Smokelong Quarterly, Callaloo, Poem/Memoir/Story, The Yale Journal of Law and Feminism, Essence magazine, and Newsday.
Thursday, October 8, 2015 - 6:30pm to 7:30pm
And West Is West (Hardcover)
By Ron Childress
Published: Algonquin Books - October 13th, 2015
The Girl Who Fell from the Sky (Paperback)
By Heidi W. Durrow
Published: Algonquin Books - January 11th, 2011