PhotoClub Scorer enables Android and IOS mobile phones and tablets to provide scoring inputs, sent over WiFi, to be transferred in real time to a Windows laptop. The PhotoClub Scorer software has been developed by Alan Boothman, who has been the Competition Secretary, and technical organiser of the South Devon International Salon, for Newton Abbot Photographic Club, in Devon UK, for many years. Any form of image presentation software can be used to progress the images, and the raw results can be further displayed in Excel. PhotoClub Scorer transfers scores directly into either Excel or DiCentra from Wilbur Imaging.
A free download, of a Windows application from my website, visiclef.net, and the low cost purchase of PhotoClub Scorer apps, from the Google Play Store and the App Store, provides clubs with a system to support mult-judge scoring, with the potential to expand to scoring by all their members as ownership of mobiles grows in the future.
Use of PhotoClub Scorer at Newton Abbot started in 2015. We use the application in conjunction with Excel spreadsheet templates, which are available in a DemoPack, or with DiCentra. The applications operate on the same desktop screen, with image scoring / image navigation driven from buttons in PhotoClub Scorer.
We run an Images of the Year competition, for both prints and PDI, making this special by using more than one judge. Often we use our own members, but, as our old electronic scoring system could not block self scoring, we did not allow our "judges" to enter. Since PhotoClub Scorer removes self-scoring, they can now enter the competition. This will be extended, in the future, to as many members as we wish, when all club members are likely to own smart devices.
We have used PhotoClub Scorer in a local four club inter-club annual battle, where each club provides one judge for the panel. We have the choice of setting the score pads to many alternative values, but choose the Salon scoring range of 2, 3, 4, 5 points from each scorer. Self-scoring, by club representatives, is again ignored, and the collected scores have been processed in either Excel or DiCentra on different occasions.
Over the years, we have run a number of different types of competition, at Newton Abbot Photographic Club, where prints have been displayed around the room, often in multi-categories, and the members score the work that we enter. In all cases, the major problem used to be speedy calculation of the results, within the time available on a club evening.
Using a screen matrix in PhotoClub Scorer, members with devices are now able to record their scores, in free time, as they walk around the room, sending them directly to our club laptop, and others, who do not have a device, use paper scoring sheets which are then transferred by those using devices. The result is then instantly available on the club laptop.
Competition preparation is minimal, since the print entries, (unknown until the night), are recorded at the door using the PhotoClub Scorer android app.
The ownership of mobile devices is becoming increasingly universal, so now is the time to give your club members the opportunity to score their fellow members work, by adding PhotoClub Scorer to your club laptop.


Cost: $5,000 yuan, $1,000 can.
Course times are Local-Time whenever possible.
Dates: Mondays from October 12 to November 30th Inclusive.
Click here for a printable PDF version of Mary Swain's Combining Intution & Therapy Doc.
Dates: Monday to Tuesday - May 1, 2017 - May 2, 2017.
This workshop will teach you how to heal relationships between husband/wife, boyfriend/girlfriend, parents and children.
Relationships have problems for several reasons. For example, we have relationship problems when we do not meet each other's expectations - you might expect your children to do certain things and they do not want to; or, you expect to have a romantic kind of relationship with your mate while s/he expects you to take care of him/her as if you were his/her mother or father. This creates problems! Relationship problems are supposed to help us improve our relationships. Once we know what to do, we can turn the crisis into greater happiness with our loved ones than we have every known before.
You will learn the causes for intimate and parent-child relationship problems. You will learn simple and practical methods to recognize why you are having relationship problems and how to cure them.
The workshop is hands-on – you will have practical experience during the workshop. For this reason, it is good if both you and family members you are having problems with can attend the workshop. If this is not possible, come by yourself, you will learn how to solve the problems on your own. This will help everyone involved.
Content of workshop includes: The 4 main causes and cures for all relationship problems How to heal relationships with husband/wife and boyfriend/girlfriend. How to heal relationships between parents and children. Why negative emotions are a part of a vital relationship for most people Why we experience emotional traumas in relationships and how to heal them You will learn how to open your heart and thereby get rid of your relationship problems. A truly open heart never has relationship problems. Why abusive relationships happen, and how to prevent them How to prevent loneliness What to do if you feel belittled or betrayed. Memories of hurts we had when we were children stay in our hearts and bodies and create our present and future relationship problems until we heal the memories. Workshop will teach you how to heal these memories.
Dates: Friday to Sunday - May 5, 2017 - May 7, 2017.
Therapists can use 4 types of knowledge: First, THEORETICAL (which does not understand an individual person or his problem). Second, LOGIC & ANALYSIS, which can only organize what we already know. Third, as a competent therapist gains experience, he uses INTELLIGENT PERCEPTION + KNOWLEDGE HE HAS GAINED THROUGH HIS PRACTICAL EXPERIENCE. Fourth, INTUITIVE KNOWLEDGE. Intuition brings new awareness. Non-intuitive psychotherapy can't get "outside the box". Intuition + psychotherapy opens therapist & client to new ways of understanding, new solutions to problems & awareness at global levels. Intuition + psychotherapy enables evolution. Evolution = true healing.
Helps clients get suffering's gifts, uses suffering to help clients evolve, not just feel better which many other forms of psychotherapy focus on. E.g. each type of painful emotion and physical disease shows us exactly how we need to evolve – once we allow the emotion/disease to help us evolve, we no longer need the negative emotion/disease.
Treats & heals all levels (physical, emotional, mental, karmic, inter-dimensional, soul, planetary, systemic, cosmic). Causes & cures are not (only) at level of symptoms – e.g. most physical diseases have emotional causes & cures; emotional problems can have physical, emotional, mental, karmic, soul, planetary, systemic and/or cosmic causes & cures.
Helps open hearts & love in best way. We suffer emotionally and mentally ONLY when our heart is not open.
Other qualities needed by intuitive psychotherapist and how to develop them (e.g. Higher Mind).
Disease and Emotional trauma help us gain greater awareness. Greater awareness is more than understanding – it is greater beingness.
Disease and emotional trauma are roads to our True Self/soul.
You will learn how to Turn Emotional Trauma into Greater Awareness & Beingness, and use it to become happier than you have ever been before.
•	Everyone has from 3-5 chronic negative emotions. Chronic negative emotions have special things to teach us.
•	It's not us who feel negative emotions, it's part of us we identify with but have outgrown. How to use negative emotions to grow into True Self.
1X PER WEEK FOR 10 WEEKS.
Dates: Day for course will be either Tuesday mornings or Wednesday mornings Montreal time, evenings China time. Day will depend when we start the class.
Identify & understand the causes. The causes of relationship problems.
What I have to say is very simple - disease is our friend. You will learn how to use the disease process to become happier than you have ever been before, understand the group nature of disease, and discover the causes and cures for diseases including the common cold, epidemics, cancer, etc.
Relationship between painful and negative emotions and physical disease. The body areas, systems and organs and their specific emotional traumas. How to take care of the body and heal painful emotions – specific R. & R., nutrition, cleansing, movements and forms of exercise for each kind of negative emotion.
29 therapies and therapeutic specifics/methods to heal painful and negative emotions: nutrition, aromatherapy, dreamwork, humour, homeopathy, colour, sound, creative therapies, pet therapy, specific bodywork methods, Bach and Australian Bush Flowers, love, journaling, self- psychotherapy and other exercises, useful books, etc.
Ultimate Cause & Cure and Emotional Trauma & Children It is not us who feel negative emotions, it is one of our personas, i.e. part of us that we are identified with, that we have outgrown. To heal, we must determine which persona or personas is/are involved, and figure out what we need to do in order to evolve it/them. This book shows how. Children do not experience emotional trauma/negative emotions the same way adults do. This part of the booklet identifies what children need in order to heal emotionally, and also gives specific methods.
INTUITIVE ART THERAPY was written to link you to the intuitive level, where both great works of art and healing on the soul level come from. The art exercises in this book will open you to intuitive awareness and self healing through feeling, colour, texture, form, and the creative process.
Spiritual beings ("guides"), rocks and crystals, essential oils for specific healing purposes. Planetary healing colours, and the colours of love. Healing mineral, plant, animal and human kingdoms. Air, water, earth, fire metal and wood elements.
Healing individuals - Diagnostics, treatments for physical, etheric, emotional and mental levels/bodies, spine clearing, chakra work.
Emotional Healing – methods, exercises and meditations to remove individual, family, national (and other group), regional and planetary fear, rage, guilt.
Healing karma – Individual, group (family, cultural, racial, social, religious, national etc.) and planetary shadow clearing. Heart, soul and shadow integration.
Introduction to soul level work – Soul to soul on-site healing. Individual, human and planetary soul healing methods. Healing the planetry etheric. Light and colour healing. Healing cell memory trauma. Cosmic solar karmic healing.
The nature of the heart. 3-fold division within the 4 areas of heart awareness. Love in the atriums and ventricles. Forms of self-centred love. Specific ways to open human hearts. Heart-opening meditation. From heart to soul. The relationship between the human heart and the current world situation. Ways to open the planetary heart.
SELF WORK FOR HEALERS Necessary areas of self work for healers and group healers, and how-to's for each area. The 3 types of karma and their relevance to healers. Chakra development specifics for healers and group healing. Effective self-help books.
1. How to use colour to evolve as a healer. What kind of colour therapist are you? What is your speific relationship to light?


So this show was better than I thought it would be, for some reason the synopsis made it seems like there would be a lot of fanservice yada yada etc. but in fact there was barely any at all.
Best OP so far this season, and really nice animation.
Of course I have to wonder if the Japs honestly think Dr. Pepper is an intellectual drink or something 'cause it's prevalent in this show as well, only with a more Japanesey name.
I tried to watch the first ep, but I dunno it never charged me and I lost interest. Lemme know how it goes.
I guess it was interesting but at the same time I had a hard time staying interested. So... maybe?
Another great episode, I wish it had gone on for an hour like the first ep but I guess I have to wait another week to see how this case turns out. Also, I realized that I can't remember the last time JC Staff made a show with an OP/ED that I didn't like, always great choices in music.


text:
Bernard Khoury
Principal architect and founder at DW5
Nadine Khalil: What does the terrible explosion say about the wider Lebanese predicament?
Bernard Khoury: I would have loved to say: Game over. Time to move on…
Nadine Khalil: Do you see it as a defining turning point in the history of the country?
BK: My sense of history for this country may be subjective, but if we are to depict it in general and abstract terms, I would say that we have been turning in circles with no set direction and most of us feel horribly dizzy and terribly disoriented.
NK: How can the creative and artistic communities move forward from now?
BK: I wish that the creative communities would start producing meaning instead of representing or aestheticizing what they observe. Artistic postures are too often built on simplistic abstractions. I am not interested in artistic practices that keep a comfortable distance from their subjects. The overwhelming majority of artists, designers and architects have the tendency to consider their practice as an autonomous discipline. This is when art limits itself to basic representations of the more complex realities that precede it. I still believe that architecture should be a political act. We have to have the courage and interest to tackle and manipulate other realities that transgress the very limited territories of our respective practices.
NK: Revisiting your 1991 conceptual project Evolving Scars, which radically commented on Solidere's reconstruction of downtown Beirut as a form of erasure, what new perspectives could the recent explosion bring?
BK: We conceive each and every project at a particular moment in time and in a very specific context. We build our schemes on our limited understanding of present realities and can only speculate and gamble on future conditions. Architects were always wrong about their predictions of the future. If many of our buildings still stand when we are six feet under, they certainly won't be playing the roles we imagined for them at the time of their conception. This is an awkward reality that most architects have a difficult time understanding because they're stuck in the Stone Age. Most of us think of buildings as permanent and impermeable structures and Bernard Khoury. PLOT #450. 2020. © DW5 Bernard Khoury. Photography by Wissam Chaaya Bernard Khoury. Evolving Scars. 1991. © DW5 Bernard Khoury that's wrong. I've helplessly seen and survived the radical and most unpredictable recuperation of many of my buildings. This has triggered in me a growing interest in intervening in those transformations. The rehabilitation of our buildings, which were affected by the blast on 4 August, will hopefully be a meaningful exercise.
NK: Should Beirut-based architects seek to recreate or restore what was there before or plan for a brave new city of the future?
BK: I believe that these projects should not be brought back to their so-called original condition. Some practitioners like mummies; others may have the courage to include the present in the making of history. When the fabric of a city and its buildings gets deeply wounded by an abrupt incident, those in charge of its recovery can resort to drastic methods. In such situations, I often compare architecture to healing with reconstructive surgery. Architectural prostheses and stitches can be effective and meaningful but also very significant.ilities.

redpajama_set_name: RedPajamaCommonCrawl


#Kitchen Idea Of The Day: Traditional Two Tone Kitchens.
2 Toned Kitchens Trendy? Too Much For Small Spaces?
Two Tone Kitchen Design With Gray Green And Light Gray Cabinets And Island.
Brown And White Two Toned Kitchen, Oh So Sophisticated!


Katharine: Love your blog and glad to hear everything is going well down there – poisonous mushrooms notwithstanding. You are very missed up here in dreary old Manhattan but its nice to get a glimpse into your new life. Take care and keep writing!


text:
Pelloloma freidbergi är en tvåvingeart som beskrevs av Kassebeer 2000. Pelloloma freidbergi ingår i släktet Pelloloma och familjen blomflugor.

Artens utbredningsområde är Kenya. Inga underarter finns listade i Catalogue of Life.

Källor

Blomflugor
freidbergi

redpajama_set_name: RedPajamaWikipedia


43 Lovely Meuble Tv Chene Ceruse is free HD wallpaper. This wallpaper was upload at September 27, 2017 upload by Diane Garcia in meubles.
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text:
Self Archiving Policies
Special Fee Waivers and Discounts
Development, Validation and Application of RP-HPLC Method: Simultaneous Determination of Antihistamine and Preservatives with Paracetamol in Liquid Formulations and Human Serum
Najmul Hasan1, *, Mathurot Chaiharn2, Umair Ali Toor1, Zulfiqar Ali Mirani3, Ghulam Sajjad4, Nawab Sher5, Mubashir Aziz6, Farhan Ahmed Siddiqui7
1 Department of Environmental Sciences and Biotechnology, Hallym University, Chuncheon, Gangwon do 200-702, South Korea
2 Division of Biotechnology, Faculty of Science, Maejo University, Chiang Mai 50290, Thailand
3 Microbiological Analytical Centre, Pakistan Council of Scientific and Industrial Laboratories Complex, Karachi, Pakistan
4 Quality Assurance, Taj Fisheries (Pvt) Ltd, Fish Harbour, West Wharf, Karachi, Pakistan
5 Department of Chemistry, Faculty of Science, University of Karachi, Karachi 75270, Pakistan
6 Department of Pathobiology, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, Pakistan
7 Faculty of Pharmacy, Federal Urdu University of Arts, Science & Technology, Karachi 75300, Pakistan
Identifiers and Pagination:
First Page: 33
Last Page: 43
Publisher Id: TOMCJ-10-33
DOI: 10.2174/1874104501610010033
Article History:
Received Date: 19/10/2015
Revision Received Date: 03/8/2016
Acceptance Date: 6/8/2016
Electronic publication date: 31/08/2016
Collection year: 2016
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Full-Text HTML Views: 2865
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© Hasan et al.; Licensee Bentham Open
open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
* Address correspondence to this author at the Department of Environmental Sciences and Biotechnology, Hallym University, Chuncheon, Gangwon do 200-702, South Korea; Tel/Fax: +82-101-2616-7470; E-mail: najam.edu.pak@gmail.com
In this article we describe development and validation of stability indicating, accurate, specific, precise and simple Ion-pairing RP-HPLC method for simultaneous determination of paracetamol and cetirizine HCl along with preservatives i.e. propylparaben, and methylparaben in pharmaceutical dosage forms of oral solution and in serum. Acetonitrile: Buffer: Sulfuric Acid (45:55:0.3 v/v/v) was the mobile phase at flow rate 1.0 mL min-1 using a Hibar® Lichrosorb® C18 column and monitored at wavelength of 230nm. The averages of absolute and relative recoveries were found to be 99.3%, 99.5%, 99.8% and 98.7% with correlation coefficient of 0.9977, 0.9998, 0.9984, and 0.9997 for cetirizine HCl, paracetamol, methylparaben and Propylparaben respectively. The limit of quantification and limit of detection were in range of 0.3 to 2.7 ng mL-1 and 0.1 to 0.8 ng mL-1 respectively. Under stress conditions of acidic, basic, oxidative, and thermal degradation, maximum degradation was observed in basic and oxidative stress where a significant impact was observed while all drugs were found almost stable in the other conditions. The developed method was validated in accordance with ICH and AOAC guidelines. The proposed method was successfully applied to quantify amount of paracetamol, cetirizine HCl and two most common microbial preservatives in bulk, dosage form and physiological fluid.
Keywords: Cetirizine HCl, Oral solution, Paraben, Paracetamol, Preservatives, RP-HPLC.
View Abstract Download PDF Download ePub
Pharmaceutical liquid preparations are particularly vulnerable to microbial growth because of the nature of their ingredients. Such preparations are protected by the addition of preservatives that prevent alteration and degradation of product formulation [1]. Hence antibacterial and antifungal properties of preservatives i.e. parabens, make them an integral part of liquid dosage formulation. Parabens that include Methylparaben (MP Fig. (1)) and propylparaben (PP, Fig. (1)), are a group of the alkyl esters of p-hydroxybenzoic acid, have a low toxicity profile and a long history of use. MP and PP are the well-known preservatives that are used primarily for their bactericidal and fungicidal properties [2]. However usage of MP is toxic at higher concentrations and has an estrogenic effect [3]. The estrogenic activity of para-bens increases with the length of the alkyl group and it is believed that PP is estrogenic to a certain degree as well [4]; therefore finished product release specifications should include an identification test and content determination test with acceptance criteria and limits for each antimicrobial preservative present in the formulation [5, 6]. These indispensable requirements encourage our team to develop new stability indicating method for simultaneous estimation of APIs along with preservatives in liquid formulations to provide driving force in today's pharmaceutical industry and clinical labs.
Fig. (1). Chemical Structure of Methylparaben (MP), Paracetamol (PCM), Propylparaben (PP) and Cetirizine HCl (CTZ).
Cetirizine dihydrochloride (CTZ, Fig. 1) is a piperazine derivative and metabolite of hydroxyzine. It is described as a long acting non-sedating antihistamine and is used for the symptomatic relief of allergic conditions including rhinitis and chronic urticaria [7]. CTZ provides prompt relief to runny nose, itching of nose or throat due to respiratory allergies, urticaria. In some allergic circumstances it is essential to co-prescribe CTZ with paracetamol (PCM, Fig. 1), in conditions such as allergic rhinitis where antihistamines are the primary medication to be used [8]. PCM, a para-aminophenol derivative, has analgesic, antipyretic and weak anti-inflammatory activity [9]. PCM combination and co-administration with CTZ is often used as anti-allergic and antipyretic, and for the treatment of severe allergies and cold [10]. Majority of cold medicines contain several active ingredients that include antipyretics, analgesics, antitussive agents, mucolytic agents and antihistamines etc. [11]. such a combination of CTZ with PCM along with MP & PP is usually found in pediatric syrups. New analytical troubles are generated by the development of new pharmaceutical forms with classical active compounds. That is the case of oral solution containing PCM, CTZ with essential presence of MP and PP as preservatives.
Literature survey reveals some analytical methods are reported for simultaneous determination of paracetamol and cetirizine HCl by high-performance liquid chromatographic (HPLC) with isocratic elution [10, 12-14], with gradient HPLC method [15-17] and capillary zone electrophoresis [18], paracetamol and parabens by HPLC [19, 20], and the one and only method for CTZ with parabens (other than PP) [21], however our extensive research discloses that till now no stability indicating isocratic HPLC method is available which can determine the four ingredients concomitantly, therefore an endeavor was made to develop a simple, sensitive and validated stability indicating RP-HPLC method using UV detection to determine PCM and CTZ along with its essential microbial preservatives in liquid dosage forms and physiological fluid. Present work deals with method development, method validation and forced degradation study of the ingredients. The applicability of the method was confirmed for analysis in pharmaceutical products and body fluid studies. The results of analysis were validated in accordance with International Conference on Harmonisation (ICH) and Association of Official Analytical Chemists (AOAC) guidelines [22, 23].
The proposed method is designed to be simple to use, sensitive, rapid with easy sample preparation for selected API's and preservatives ingredients. Separation and quantification in pharmaceutical drug formulations and blood were achieved with an isocratic elution.
2.1. Reagents and Chemicals
Cetirizine HCl, paracetamol, methylparaben and propylparaben were kind gifts from national pharmaceuticals, whereas sulphuric acid, sodium heptane sulphonate, acetonitrile (HPLC grade) were purchased from Merck (Germany). From commercial source pharmaceutical dosage forms containing the respective ingredients were obtained including Novopyrin® (Specific Research Laboratories), Rigix Oral Solution® (AGP (Pvt) Ltd.), Linazine® (Alina Combine Pharmaceuticals (Pvt) Ltd.), and Pedrol®(Stanley Pharmaceuticals (Pvt) Ltd.).
2.2. Instruments
For chromatography a SIL 20AHT auto injector HPLC system comprising of CBM-20A controller, SPD 20A prominence UV/VIS detector, and Shimadzu LC 20 AT Prominence LC pump with software LC Solutions version 1.25 was used. Separation was performed on a Hibar® Lichrosorb® ODS C18 HPLC column, (4.6 x 250 mm; 10µm bead size) maintained at 25°C. A UV-visible Shimadzu 1650 PC spectrophotometer with UV Probe V2.0 software, ultrasonic cleaner (Elmasoni E 60 H), pH meter (Jenway 3240), shaker (Labnet Orbit 1000), centrifuge machine (HERMLE Z200A) and analytical balance (Sartorious TE2145) were used in the research work. Throughout the work only Class-A (Pyrex®) calibrated volumetric flasks and pipettes were used, as guided by Chan, Chung Chow et al. [24].
2.3. Chromatographic Conditions
HPLC analysis was carried out at ambient temperature. Compounds were chromatographed isocratically with a mobile phase consisting of acetonitrile: sodium n-heptane sulfonate 0.01M: sulfuric acid 0.1M (45:55:0.3 v/v/v) with apparent pH 3.0 ± 0.1 (pH adjusted if required using sulfuric acid 0.1M). Mobile phase was filtered by passing through a 0.45 µm membrane filter (Millipore, Bedford, MA, USA). Flow rate of mobile phase was 1.0 mL min-1, and injection volume of testing solution was 20µL. The effluent was monitored spectrophotometrically at wavelength of 230nm. Mobile phase was used as dilution solvent, throughout the analytical work.
3. EXPERIMENTAL
3.1. Standard Preparation
3.1.1. Stock Solutions
In a 100 mL class-A volumetric flask 15mg of PP reference standard was accurately weighed and dissolved in dilution solvent via sonication for few seconds (usually 10-15 seconds) to have a stock solution-A of 150 µg mL-1. Similarly in three other flasks 10mg, 135mg and 100mg of CTZ, MP and PCM were dissolved in diluent to get stock standard solutions of 100 µg mL-1, 1350 µg mL-1 and 1000 µg mL-1 concentration respectively. These stock solutions were further diluted for performing different experiments in the research work. For dilution and other solution preparation purposes calibrated class-A (Pyrex®) minimal volume pipette of 10 mL was used [24].
3.1.2. Lab. Prepared Mixture Solutions
For laboratory mixture preparation 15mg of PP reference standard was accurately weighed and dissolved in dilution solvent via sonication for few seconds to have a stock solution-A of 150 µg mL-1 . While in another class-A 100 mL volumetric flask, CTZ & MP reference standards were accurately weighed as 10mg and 13.5mg added by 10 mL of stock solution-A and dissolved in diluent and designated as stock solution-B. Similarly in another 100 mL volumetric flask, accurately 10mg of PCM was weighed and dissolve in 50 mL of dilution solvent; added by 10 mL of the stock solution-B and filled up to the mark with dilution solvent to get stock solution-C containing 10 µg mL-1 of CTZ, 100 µg mL-1 of PCM, and 13.5 µg mL-1 of MP and 1.5 µg mL-1 of PP. This working solution was used in further analytical steps.
3.2. Sample Preparation
3.2.1. Marketed Samples
For making a sample from syrups, one bottle of each brand was opened and content was mixed to get an evenly homogenized stock sample to contain 100 µg mL-1, 1350 µg mL-1, 1000 µg mL-1 and 150 µg mL-1 of CTZ, MP, and PCM & PP respectively. Sample volume of 10 mL was accurately taken in 100 mL volumetric flask and 50 mL of diluent was added. Then sample was mixed thoroughly by stirring for 10 minutes and added by diluent up to the mark and designated as stock sample-A; second dilution was prepared by dissolving 10 mL of stock sample-A in 100 mL diluent and then filtered through 0.45 µm filter and injected into HPLC system.
3.2.2. Sample for Serum
By a skilled clinical laboratory technician blood samples were collected from healthy volunteers in evacuated glass tube. Volunteers were confirmed to be not involved in any medication, smoking, and strenuous activity. In order to separate out serum, blood was shaken with the aid of shaker (Labnet Orbit 1000) at 200 rpm and centrifuged by centrifuge machine at 10,000 rpm for 15 minutes. Acetonitrile was added to serum (90:10, v/v) and centrifuged again at 10,000 rpm for 10 min to deprotonate it. The serum thus obtained was used for analysis and was stored at 20°C. For making working sample 10 mL of stock sample-A was taken in 100 mL flask, followed by 15 mL of serum. The sample thus obtained was stirred for 10 minutes and added with diluent up to the mark. Triplicate solutions were prepared for each working solution for analysis in serum, and were filtered through 0.45 µm filter prior to inject into HPLC system.
3.2.3. Forced Degradation Conditions
For this purpose 10 mL of stock solution-A was diluted in four individual 100 mL volumetric flasks and 15 mL of degrading agent were added to each flask individually, with exception of one to which only diluent was added; these included 0.1M HCL, 0.1M NaOH, 3%H2O2 and then to each flask diluent was added up to the mark. All the four samples were placed in water bath at 60°C for one hour. Samples were then filtered through 0.45 µm filter paper and injected into HPLC system.
3.2.4. Stability Studies
For stability studies commercially available samples were placed at accelerated conditions of temperature and humidity that is at 40°C with 75% relative humidity and at ambient conditions of 30°C temperature with 65% relative humidity in environmental chamber for six months. A stability protocol was followed for six months and assays were performed as described previously.
3.3. Method Validation
Method validation was performed following ICH and AOAC guidelines [22, 23] according to which assay validation was performed via various procedures including specificity, linearity, range, accuracy, intra-day & inter-day precision and system suitability etc. To study linearity, twenty dilutions were prepared from stock solution-B to give standard solutions in range of 10% to 200% of all ingredients content for marketed products. Standard calibration curve was generated using regression analysis. For specificity commonly used excipient in syrup preparation were spiked in a pre-weighed quantity of drugs and then peak areas were measured and calculated to determine the quantity of the drugs recovered. Precision was performed by analyzing corresponding standard daily for a period of three days (Inter-day precision or reproducibility), and three times a day with an interval of 08 hours (Intra-day precision or repeatability) against freshly prepared standard solutions. For determining accuracy reference standards were accurately weighed and added to previously analyzed syrup sample, to get three different concentration levels i.e. 110%, 120%, 130% of the API's. At each level, samples were prepared in triplicate and recovery percentage was determined.
Limit of detection (LOD) and limit of quantification (LOQ) for the method were established by sequentially diluting standard solutions at decreasing concentrations, in range of 100 ng mL-1 to 0.01 ng mL-1 and injected onto chromatographic system. Limit of detection was defined as the concentration for which a signal-to-noise ratio of 3:1 was obtained and, for quantification limit; a signal-to-noise ratio of 10:1 was considered. Robustness was studied by analyzing same samples of syrup by deliberate variation in method parameters, such as in the chromatographic conditions, like mobile phase, pH, flow rate, temperature etc. System suitability of the method was evaluated by analyzing symmetry of the standard peaks, resolution and theoretical plates of the column.
HPLC method development and its validation are the obligatory requirements for any drug available in the market to have high quality products. A few methods are available for determination of CTZ and PCM as described earlier, but many of them are used only for certain definite objectives and none can be generalized for simultaneous determination of CTZ and PCM with mentioned preservatives in pharmaceutical products and serum. In order to solve the problems of the gradient elution, we developed and validated an isocratic HPLC method for the simultaneous determination of the four ingredients.
Similarly none of the mentioned methods [10, 17] is as much sensitive as ours; in terms of its precision, accuracy, %recovery, limit of detection LOD and limit of quantification LOQ. Moreover this method is sensitive enough to be used for pharmacokinetic studies as its LOD & LOQ are in nano gram range, and provide best resolution of drugs. This section of the research work is part of our future article to be published soon afterwards.
4.1. Method Development & Optimization
For developing a fit-to-purpose method for analysis, parameters, such as detection wavelength, mobile phase composition, optimum pH and concentration of the standard solutions were comprehensively studied. All the ingredients were diluted in dilution solvent and then run through UV spectrophotometer (Shimadzu 1650 PC) in UV range of 190nm-400nm to get maximal wavelengths, where maximum absorbance was attained i.e. 230nm, 220nm, and 254nm for CTZ, PCM and MP & PP respectively. However considering the difference of concentration of the ingredients and intensity of their absorbance a single wavelength method was adopted, that is 230nm at which all the molecules gave satisfactory absorbance. The chromatographic parameters were evaluated using a Hibar® Lichrosorb® C18 column. An ion-pairing mobile phase already developed by our research team [25] was selected in terms of its components & their proportions and was modified for better representable chromatography. The mobile phase composed of Acetonitrile: Buffer of previously mentioned proportion promoted a short run time (10 min) as shown in Fig. (2), so this condition was adopted in accompanying analysis.
4.2. Validation Studies
The linearity was determined in the range of 10% - 200% of claimed content for all API's in marketed formulation. The assay was judged to be linear as the correlation coefficient was greater than 0.997 in all cases, calculated by the least-square method. A linear correlation was found between the peak areas and the concentrations, in the assayed range. The regression analysis data are presented in Table 1.
Table 1. Calibration curve data and validation parameters.
Linearity range (µg mL-1) 10-200 1.35 27 0.15 - 3 1 - 20
Correlation coefficient (r) 0.9998 0.9984 0.9997 0.9977
Regression equation (y=mx+c) Slope (m) 0.096948 0.099248 0.096796 0.10153
Intercept (c) 0.140771 -43487 0.153070 -0.2371
Limit of detection LOD (ng mL-1) 0.5 0.8 0.1 0.5
Limit of quantification (LOQ) (ng mL-1) 2 2.7 0.3 2
Chromatogram shown in Fig. (2b) proves specificity or selectivity of the assayed method, as the chromatograms in samples were found identical with standard chromatogram (Fig. 2a) and no interference peak was observed either in pharmaceutical formulation or in physiological fluid. Peak purities higher than 97.0% (Tables 2 and 3) were obtained from assays of sample solutions, demonstrating that other compounds did not co-elute with peaks of interest. The chromatogram obtained with the mixture of the syrup excipient (Fig. 2b) proves that there is no interference from excipients thus peak of interest fulfills all the requirements of symmetrical peak, and hence the specificity is established.
Precision of an analytical method is the degree of coherence among individual test results when the method is applied repeatedly to multiple sampling of homogeneous bulk. Intra-day precision of the method was evaluated at three different independent concentrations i.e. 80%, 100%, and 120% for the drugs (n=3) by determining their assays. Inter-day precision of the method was tested for 3 days at the same concentration levels. Solutions for calibration curves were prepared every day on freshly basis. Since the inter-day and intra-day precision obtained %RSD was less than 2% it assures that the proposed method is quite precise and reproducible as shown in Table 2.
Table 2. Inter-day and Intra-day precision and recovery studies.
Nominal concentration of active drugs (µg mL-1)
Inter-Day RSD%
PCM 40 99.8 99.2 99.5 99.51 0.33
50 100.9 98.2 99.3 99.47 1.36
60 100.7 101.4 101.0 101.02 0.35
Mean 100.48 99.59 99.93
Inter-Day RSD% 0.57 1.65 0.91
MP 1.08 99.5 98.9 99.3 99.23 0.31
1.35 100.6 99.1 99.7 99.80 0.76
1.62 99.7 99.3 100.3 99.77 0.50
Mean 99.93 99.10 99.77
PP 0.12 98.7 99.1 97.9 98.57 0.62
0.15 99.6 98.5 98.1 98.73 0.79
CTZ 0.8 98.7 99.1 99.5 99.10 0.40
1 99.3 99.7 98.8 99.27 0.45
1.2 99.0 99.3 100.8 99.70 0.97
Table 3. Contents of PCM, MP, PP and CTZ in commercial samples.
Commercial Samples
Content (%) ± S.D.*
Rigix Oral Solution® N/A 98.2± 0.75 99.1± 0.39 98.7± 0.44
Linazine® N/A 97.17 ± 0.29 99.84 ± 0.76 98.79 ± 0.87
Pedrol[REMOVED HYPERLINK FIELD]® 99.67± 0.95 100.59± 0.68 100.46± 0.74 N/A
Novopyrin ® 100.88 ± 0.87 99.37 ± 0.85 98.65 ± 0.64 N/A
Accuracy was investigated by means of addition of reference standards to a mixture of the syrup excipients. Recovery studies of the drug were carried out for the accuracy parameter at three different concentration levels i.e. multiple level recovery studies. A known amount of API's standards were added into pre-analyzed sample and subjected to proposed HPLC method. The mean recovery (n = 9) was 99.0% - 99.7% (RSD=0.61%) for CTZ, 99.51% - 101.02% (RSD=0.68%) for PCM, 99.23% - 99.93% (RSD=0.52%) for MP and for PP 99.0% -98.47% (RSD=0.61%), demonstrating the accuracy of the method. Percentage recoveries for marketed products were found to be within the limits (Table 3).
The statistical analysis showed no significant difference between results obtained employing the analytical conditions established for the method and those obtained in the experiments in which variations of some parameters were introduced. The parameters used in system suitability test were symmetry of peaks, tailing factor, resolution and RSD of peak area for replicate syrup. Thus, the method showed to be robust for changes in mobile phase acetonitrile proportion, mobile phase pH, flow rate, and column temperature (Table 4).
Fig. (2). (a) Typical Chromatogram of standard (b) marketed sample 1-Pracetamol (PCM) 2-Cetirizine HCl (CTZ) 3-Methylparaben (MP) and 4-Propylparaben (PP).
Table 4. Robustness of method.
Chromatographic Conditions
Retention time (Minutes)
Temprature (ºC) 23 3.42 4.64 6.63 7.609
27 3.57 4.69 6.8 7.701
Flow rate (mL min-1) 0.8 3.75 4.56 6.48 7.31
Vol. of Acetonitrile (%) 43 3.67 4.71 6.75 7.83
47 3.37 4.54 6.56 7.55
4.3. Forced Degradation Studies
Forced degradation is a process that involves degradation of drug products and drug substances at conditions more severe than accelerated conditions for stability and thus generates degradation products that can be studied to determine stability of the molecule. ICH guideline states that stress testing is intended to identify the likely degradation products which further helps in determination of the intrinsic stability of the molecule, establishing degradation pathways and to validate the stability indicating procedures used [22]. Forced degradation has to demonstrate specificity when developing stability-indicating methods and for this reason, it should be performed prior to implementation of stability studies to assure that analytical methods are stability-indicating [26], hence a forced degradation study was performed. During this study, upon treatment of CTZ, PCM, MP and PP with alkali (0.1M NaOH), acid (0.1M HCl), hydrogen peroxide (3%) and heat, it was observed that CTZ and PCM with preservatives are fairly stable to alkaline, oxidative and thermal stress conditions as the degradation was less than 10% that could be considered as allowed value for a product whose shelf life limits is in 90% - 110% range [27, 28]. In Fig. (3) samples showed different grades of degradation based on forced degradation conditions, in some cases higher than the individual components due to formation of some degradation products by combination of several of them.
Fig. (3). Forced degradation studies: (a) Alkali treated (b) Acid treated (c) Heat treated sample (d) H2O2 treated.
In our study PCM proved to have stability on thermal stress condition and no extra peak or increased area was observed which is in agreement with previous studies [29]. While oxidation with peroxide gave an extra peak, supposed to be benzoquinine-imine derivative (Fig. 3d), however, about 13% increased which is much more than observed by earlier researchers [26, 29] whereas about 4% decrease was observed for CTZ, MP and PP each under same stress condition. In alkaline conditions more PCM degradation was observed, which infers that greater oxidative degradation of PCM is attributed to oxidative vulnerability pushed up by electron withdrawing group in the form of amide as compared to hydrolysis. Whereas the other ingredients showed somewhat same behavior as in oxidative stress condition. A negligible degradation was observed for all the APIs (Fig. 3b) when acidic hydrolysis was performed which shows the same pattern as in the thermal stress condition. Esters of MP, PP and salt of carboxylic acid showed thermal stability which is similar to the results obtained in an earlier work [2]. Although parabens do possess carboxyl group but more drastic conditions are required to get them degraded as compared to PCM and CTZ. They have been found comparatively stable in all the provided conditions however as shown in the Fig. (3a and b) strong hydrolysis with both acid and base along with heating for longer time is required to decarboxylate the molecules to some extent, always not found on common experimental condition.
As shown by the chromatograms (Fig. 3) acidic and basic hydrolysis of paracetamol degraded peaks appear to be on the same retention time that can be assumed to be amino phenol which is the outcome of decarboxylation reaction after hydrolysis. The data in Table 5 also revealed that PCM and CTZ are usually more vulnerable to the stress conditions as compared to MP and PP. Further in Fig. (3a), base treated sample, degraded peaks of PCM is the Clear evidence of base hydrolysis of carboxylic group. Extremely high absorbance PCM in peroxide treated sample (Fig. 3d) showing the sensitivity of carboxylic salt being oxidized. The degraded peak appears to be on the same retaining time which can be assumed to be amino phenol and which is the outcome of decarboxylation reaction after hydrolysis.
Table 5. Summary of forced degradation results.
Stress conditions
Assay of active substance affected due to stress condition (%)
Assay of active substance under normal conditions (%)
Acid hydrolysis
(0.1 M HCl) 60 101.49 101.7 103.4 102.6 99.467 99.800 98.733 99.267
Base hydrolysis
(0.1 M NaOH) 60 110.276 102.243 104.335 105.096
(3% H2O2) 60 113.784 96.722 96.245 96.688
Thermal (60ºC) 60 100.53 105.47 104.38 105
4.4. Stability Studies
Stability testing is an imperative part of the process of drug product development. Major purpose of stability testing is to provide documented data of how the quality of a drug substance or drug product varies with time under a variety of environmental conditions, for example temperature, humidity, and light and enables recommendation of storage conditions, retest periods, and shelf life to be established. It is a well-established fact that main aspects of drug product that play an important role in shelf-life determination are assay of the active drug and the degradation products generated during stability studies. Therefore it has become mandatory to perform stability studies of new drug moiety before filing in registration dossier. The stability studies include long term studies (12 months) and accelerated stability studies (6 months). But intermediate studies (6 months) can be performed at conditions milder than that used in accelerated studies [30]. The proposed assay method was applied to stability study of commercially available syrups, for which the samples were placed at specified condition and study was proceeded according to stability protocol as described in previous section (Table 6). Samples were analyzed and percentage of contents was measured. According to results obtained CTZ and PCM with preservatives were found to be stable at applied conditions of temperature and relative humidity, and were accurately analyzed with the proposed method.
Table 6. Summary of stability studies.
TEST (Claimed content)
RSD%
Stability Studies at Accelerated (40ºC+75%H)
PCM 99.467 98.769 98.628 98.591 97.286 98.139 97.528 98.344 0.767
MP 99.800 99.576 99.783 100.546 99.826 98.763 98.375 99.524 0.732
PP 98.733 98.527 98.386 97.672 97.482 97.135 96.628 97.795 0.800
CTZ 99.267 99.626 99.295 99.386 98.492 99.184 98.783 99.148 0.388
Stability Studies at Long Term (30ºC+65%H)
MP 99.800 99.536 99.638 99.318 99.107 98.836 98.937 99.310 0.369
A new HPLC method is proposed for simultaneous determination of cetirizine HCl, paracetamol with preservatives methylparaben and propylparaben in serum and liquid dosage forms. The proposed HPLC method described provides a simple, convenient and reproducible approach for simultaneous identification and quantification of four ingredients in human serum and pharmaceutical formulations with good separation and resolution. In addition, this method has the potential application to clinical research of drug combination. Analytical results are accurate and precise with good recovery and lowest detection limit values. In short, the developed method is simple, sensitive, easy, and efficient having short chromatographic time and can be used for routine analysis in QC laboratory and therapeutic monitoring. Due to the presence of different interferents in solution formulations such as coloring agents, flavoring agents, sweetening agents and preservatives other than PP and MP, the proposed method should be re-evaluated prior usage for commercial solution products containing ingredients other than those used in this work.
(AOAC) = Association of Official Analytical chemists
(CTZ) = Cetirizine HCl
(HPLC) = High Performance Liquid Chromatography
(ICH) = International Conference on harmonization
(MP) = Methylparaben
(PCM) = Paracetamol
(PP) = Propylparaben
The authors confirm that this article content has no conflict of interest.
Declared none.
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The Open Medicinal Chemistry Journal
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Dr. Ernest A. Adeghate is presently associated with United Arab Emirates University (Department of Human Anatomy, College of Medicine and Health Sciences), Al-Ain, United Arab Emirates. His major subject areas are Biochemistry, Genetics, Molecular Biology, Pharmacology, Toxicology, Pharmaceutics, Neuroscience, Agricultural and Biological Sciences, Chemistry, Immunology, Microbiology, etc. Dr. Adeghate has contributed more than 200 publications, having over 3,225 total citations.
Department of Anatomy, College of Medicine & Health Sciences, UAE University, Al Ain, United Arab Emirates
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And I will be seeing it again, feeling that shiver of excitement, on our Travel Club Tour Antarctica. It's the experience of a lifetime.
I've led a group there before. I can't wait to do it again.
After time in the beautiful city of Buenos Aires, which I know well, we'll head south to Ushuaia, at the southern tip of South America. It's one of my favourite places, with its "end of the world" feel.
Then we'll board a great expedition ship and cross the Drake Passage to the Antarctic Peninsula.
The Drake — that "pinch point" in the great rotation of the great oceans. I love being on deck watching the wandering albatrosses and perhaps seeing whales pass and penguins speed past like wind-up toys ... dozens of them flying in little arcs over the water, vanishing underneath, then back again.
Once we have arrived on the continent, there will be penguins, of course.
They're brilliant, and there are some 300,000 breeding pairs of gentoos.
There will be crabeater and Weddell seals and, quite possibly, an encounter with a leopard sea. They swim like a torpedo.
We will be out and about in Zodiacs, among the icebergs and brash ice and landing on the Peninsula.
Of course, a great part of an Antarctic voyage for many is photography.
I'll run two sessions before we go — one general event to prepare people for the journey and a specific photography workshop for those who want to prepare their skills.
We'll be aboard a fantastic ship — the National Geographic Explorer — and accompanied by some of the world's best Antarctic experts, who will be keen to share their knowledge and experiences.
And I will be there, every step of the way, enjoying the great, white continent.
Journey to Antarctica: The White Continent is in partnership with Collette.
I will be with the group throughout.
The whole journey includes Buenos Aires, Ushuaia, Drake Passage, authentic expedition ship, Zodiac forays, penguin colony close-ups, summit hike, kayak excursions, photography guidance and on-board information sessions.
After flying to Buenos Aires (day 1), we will stay in the Alvear Art Hotel (days 2-3), continuing to Ushuaia to join the National Geographic Explorer (days 4 to 13) and returning to Buenos Aires (day 14).
Antarctica is a once-in-a- lifetime experience, and this is a fully stabilised, very comfortable expedition ship with world-class experts, and fresh and varied cuisine. With a maximum of 148 passengers, it is a great base.
The 15-day trip includes flights, 36 meals, one of the world's best expedition cruise ships and an expert team. The all-inclusive fare is from $24,395 per person, twin share.


Watch the What's in My Baby's Hospital Bag video here!
If you saw yesterday's post you'll know that I just showed you guys what I packed in my hospital bag for labour & delivery...well here's what's in Emilia's bag! :) Hers is much prettier and fun, plus I had a way better time packing it. All of her stuff is just so cute!
I had so much fun packing Emilia's bag and hopefully you guys will find this post interesting or helpful if you're in a similar situation! :) Now I just can't wait for her to get here!!
Lovely things! You may find johnson will cause allergy. Then it is good to hi for oilatum stuff for babies!
Wow. How long will you be staying in the hospital. Here in the US it's 2 days for a vaginal delivery and 3 days for a c-section. Of course if there is complications it's longer but the baby normally stays in the hospital issued onsie. I'll be having my third in November. I never needed any of that stuff. They will bathe the baby once and then you can do it once you get home or a few days after. Too many baths so young will dry out their skin.


We want it all. Have fun, relax, time to get our work done, work out, and sleep. Even though sleep is last in that order, it should be at the top of your list. Without a healthy amount of sleep you are likely to lack or show up with out the right energy to the rest of your activities. If you can't get to sleep on time, here are some tips to help make the time you do sleep, better.
If you are a coffee drinker, do your best to stay away from it after lunch. Caffeine wakes up your body and not your brain so by taking it you will only make yourself restless later on.
Netflix is the downfall of many a procrastinators motivation. With the automatic queue you can finish a whole season in a day. But when it's time to go to bed, get rid of anything that produces a lot of light as well as calls your attention. This will help bring the z's.
the middle of the night to use the restroom.
The last tip is tricky. Use your internal alarm clock. When you have your routine its simple. Sleep when your body is telling you to. When you sleep better, waking up is easier.
If you want your dreams to come true, get better rest so you can have the energy to make those dreams a reality.


text:
Tilt  is a poker term for a state of mental or emotional confusion or frustration in which a player adopts a suboptimal strategy, usually resulting in the player becoming overly aggressive. Tilting is closely associated with another poker term, "steam".

Placing an opponent on tilt or dealing with being on tilt oneself is an important aspect of poker.  It is a relatively frequent occurrence due to frustration, animosity against other players, or simply bad luck.

The term originated from pinball where physically tilting the machine causes some games to flash the word "TILT" and freeze the flippers.

Tilt is also common in other games, especially chess and esports. Tilting in esports causes players to "lose control due to anger". The most extreme reaction is termed a "ragequit", angrily leaving the match or quitting the game, physically turning off the device or breaking a part like a monitor or control device by smashing it.

Common causes of tilt
The most common cause of tilt is losing, especially being defeated in a particularly public and humiliating fashion. In poker, a bad beat can upset the mental equilibrium essential for optimal poker judgment, causing frustration. Another common cause of tilt is bad manners from other players causing frustration which eventually leads to tilting.

Though not as commonly acknowledged or discussed, it is also quite possible to go on "winner's tilt" as a result of a positive trigger: such as winning unexpectedly, or going on a string of good luck. Strong positive emotions can be just as dizzying and detrimental to one's play as negative ones. Tilting and winner's tilt can both lead to the same habits.

Advice when tilted
For the beginning player, the elimination or minimization of tilt is considered an essential improvement that can be made in play (for instance in the strategic advice of Mike Caro). Many advanced players (after logging thousands of table-hours) claim to have outgrown "tilt" and frustration, although other poker professionals admit it is still a "leak" in their game.

One commonly suggested way to fight tilt is to disregard the outcomes of pots, particularly those that are statistically uncommon. So-called "bad beats," when one puts a lot of chips in the pot with the best hand and still loses, deserve little thought; they are the product of variance, not bad strategy. This mindset calls for the player to understand poker is a game of decisions and correct play in making the right bets over a long period of time.

Another method for avoiding tilt is to try lowering one's variance, even if that means winning fewer chips overall. Therefore, one may play passively and fold marginal hands, even though that may mean folding the winning hand. This may also imply that one plays tightly— and looks for advantageous situations.

Once tilt begins, players are well-advised to leave the table and return when emotions have subsided. When away from the table, players are advised to take time to refresh themselves, eat and drink (non-alcoholic) if necessary, and take a break outside in the fresh air.

If none of these work in lessening tilt, players are advised to leave the game and not return to playing until they have shaken off the results that led to the tilt.

The intent of the advice is to prevent the upset person from letting negative emotions lead to bigger losses that can seriously hurt one's bankroll.

Tilt must be taken seriously, requiring immediate attention following its presence. The progression in poker for chronically tilted players may be significantly hindered as their judgement becomes progressively impaired as agitation becomes more prominent. Paying close attention to playing statistics can assist in preventing this, as a statistical overview of recent hands can reduce the players likelihood to play impulsive hands habitually.

Tilting others

The act of putting an opponent on tilt may not pay off in the short run, but if some time is put into practicing it, a player can quickly become an expert at "tilting" other players (with or without using bad manners). In theory, the long-run payoff of this tactic is a monetarily  positive expectation.

Common methods of putting a table on tilt include:
 Playing junk hands that have a lower chance of winning in the hope of either sucking out and delivering a bad beat (which can be an enjoyable occasional style which will make the table's play "looser") or bluffing the opponent off a better hand (with the option of showing the bluff for maximum tilting effect).
 Victimising individuals at the table, (which is often considered a more old-fashioned tactic, identified with 1970s "verbal" experts such as Amarillo Slim.)
 Pretending intoxication, i.e. hustling, excellently demonstrated by Paul Newman against Robert Shaw in The Sting (although his technique included cheating).
 Constant chattering, making weird noises and motions whenever you win a hand, or other erratic behavior is a "tilting" or "loosening" approach first discussed by Mike Caro.
 Taking an inordinate or otherwise inappropriate amount of time to announce and show your hand (also called "slow-rolling") at the showdown. (Such deliberate breaches of etiquette have the side effect of slowing play and risking barring, thereby limiting the earnings of the expert player. For this, and other social reasons, such tactics are mostly associated with novices.)

These antics can upset the other players at the table with the intention of getting them to play poorly.

See also
Cognitive bias
Glossary of poker terms
Tilting at windmills

References

External links

 Help for problem gambling at NHS (UK)

Poker gameplay and terminology


text:
Q: How to banned a token in LexikJWTAuthenticationBundle? When a user wants to quit the application, it is required to ban his token, since it is still valid.

A: This is just the approach I am using for my application
Just provide a new authenticator for all or the desired routes and rewrite its loadUser. This is how I do it
Provide your authenticator for any firewall in your security.yml
    firewalls:
    #...
    #some firewalls
    #...

    main:
        pattern: ^/
        stateless: true
        switch_user_stateless: true
        guard:
            authenticators:
                - mylexik_jwt_authentication.security.guard.jwt_token_authenticator
        anonymous:    false

As you can see you can define  your own authenticator to any firewall or all of them( I do it). In this case the one I provided is an exact copy of the lexik authenticator, but with the loadUser method redefined( Perhaps is possible redefine just the UserProvider instead the whole authenticator I dont know).
Register your authenticator as a service in services.yml
       mylexik_jwt_authentication.security.guard.jwt_token_authenticator:
       class: SeguridadBundle\DependencyInjection\MyJWTTokenAuthenticator
       arguments: ["@lexik_jwt_authentication.jwt_manager", "@event_dispatcher", "@lexik_jwt_authentication.extractor.chain_extractor"]
       calls:
            - [setContainer, ["@service_container"]]

And this is the code of my authenticator, notice is exactly the same except for the loadUser method. (Better extend from JWTTokenAuthenticator and just redefine the loadUser method, cleaner solution)
   <?php

namespace SeguridadBundle\DependencyInjection;

use AplicacionBaseBundle\Controller\EmpresaController;
use Lexik\Bundle\JWTAuthenticationBundle\Event\JWTAuthenticatedEvent;
use Lexik\Bundle\JWTAuthenticationBundle\Event\JWTExpiredEvent;
use Lexik\Bundle\JWTAuthenticationBundle\Event\JWTInvalidEvent;
use Lexik\Bundle\JWTAuthenticationBundle\Event\JWTNotFoundEvent;
use Lexik\Bundle\JWTAuthenticationBundle\Events;
use Lexik\Bundle\JWTAuthenticationBundle\Exception\ExpiredTokenException;
use Lexik\Bundle\JWTAuthenticationBundle\Exception\InvalidPayloadException;
use Lexik\Bundle\JWTAuthenticationBundle\Exception\InvalidTokenException;
use Lexik\Bundle\JWTAuthenticationBundle\Exception\JWTDecodeFailureException;
use Lexik\Bundle\JWTAuthenticationBundle\Exception\MissingTokenException;
use Lexik\Bundle\JWTAuthenticationBundle\Exception\UserNotFoundException;
use Lexik\Bundle\JWTAuthenticationBundle\Response\JWTAuthenticationFailureResponse;
use Lexik\Bundle\JWTAuthenticationBundle\Security\Authentication\Token\JWTUserToken;
use Lexik\Bundle\JWTAuthenticationBundle\Security\Authentication\Token\PreAuthenticationJWTUserToken;
use Lexik\Bundle\JWTAuthenticationBundle\Security\User\JWTUserProvider;
use Lexik\Bundle\JWTAuthenticationBundle\Services\JWTTokenManagerInterface;
use Lexik\Bundle\JWTAuthenticationBundle\TokenExtractor\TokenExtractorInterface;
use SeguridadBundle\Controller\UsuarioController;
use SeguridadBundle\DependencyInjection\Helpers\GroupHelper;
use SeguridadBundle\DependencyInjection\Helpers\UserHelper;
use Symfony\Component\DependencyInjection\ContainerAwareInterface;
use Symfony\Component\DependencyInjection\ContainerAwareTrait;
use Symfony\Component\EventDispatcher\EventDispatcherInterface;
use Symfony\Component\HttpFoundation\Request;
use Symfony\Component\Security\Core\Authentication\Token\Storage\TokenStorage;
use Symfony\Component\Security\Core\Authentication\Token\Storage\TokenStorageInterface;
use Symfony\Component\Security\Core\Authentication\Token\TokenInterface;
use Symfony\Component\Security\Core\Exception\AuthenticationException;
use Symfony\Component\Security\Core\Exception\UsernameNotFoundException;
use Symfony\Component\Security\Core\User\UserInterface;
use Symfony\Component\Security\Core\User\UserProviderInterface;
use Symfony\Component\Security\Guard\AbstractGuardAuthenticator;

/**
 * JWTTokenAuthenticator (Guard implementation).
 *
 * @see http://knpuniversity.com/screencast/symfony-rest4/jwt-guard-authenticator
 *
 * @author Nicolas Cabot <n.cabot@lexik.fr>
 * @author Robin Chalas <robin.chalas@gmail.com>
 */
class MyJWTTokenAuthenticator extends AbstractGuardAuthenticator implements ContainerAwareInterface
{
    use ContainerAwareTrait;
    /**
     * @var JWTTokenManagerInterface
     */
    private $jwtManager;
/**
 * @var EventDispatcherInterface
 */
private $dispatcher;

/**
 * @var TokenExtractorInterface
 */
private $tokenExtractor;

/**
 * @var TokenStorageInterface
 */
private $preAuthenticationTokenStorage;

/**
 * @param JWTTokenManagerInterface $jwtManager
 * @param EventDispatcherInterface $dispatcher
 * @param TokenExtractorInterface $tokenExtractor
 */
public function __construct(
    JWTTokenManagerInterface $jwtManager,
    EventDispatcherInterface $dispatcher,
    TokenExtractorInterface $tokenExtractor
)
{
    $this->jwtManager = $jwtManager;
    $this->dispatcher = $dispatcher;
    $this->tokenExtractor = $tokenExtractor;
    $this->preAuthenticationTokenStorage = new TokenStorage();
}

public function supports(Request $request)
{
    return false !== $this->getTokenExtractor()->extract($request);
}

/**
 * Returns a decoded JWT token extracted from a request.
 *
 * {@inheritdoc}
 *
 * @return PreAuthenticationJWTUserToken
 *
 * @throws InvalidTokenException If an error occur while decoding the token
 * @throws ExpiredTokenException If the request token is expired
 */
public function getCredentials(Request $request)
{
    $tokenExtractor = $this->getTokenExtractor();

    if (!$tokenExtractor instanceof TokenExtractorInterface) {
        throw new \RuntimeException(sprintf('Method "%s::getTokenExtractor()" must return an instance of "%s".', __CLASS__, TokenExtractorInterface::class));
    }

    if (false === ($jsonWebToken = $tokenExtractor->extract($request))) {
        return;
    }

    $preAuthToken = new PreAuthenticationJWTUserToken($jsonWebToken);

    try {
        if (!$payload = $this->jwtManager->decode($preAuthToken)) {
            throw new InvalidTokenException('Invalid JWT Token');
        }

        $preAuthToken->setPayload($payload);
    } catch (JWTDecodeFailureException $e) {
        if (JWTDecodeFailureException::EXPIRED_TOKEN === $e->getReason()) {
            throw new ExpiredTokenException();
        }

        throw new InvalidTokenException('Invalid JWT Token', 0, $e);
    }

    return $preAuthToken;
}

/**
 * Returns an user object loaded from a JWT token.
 *
 * {@inheritdoc}
 *
 * @param PreAuthenticationJWTUserToken Implementation of the (Security) TokenInterface
 *
 * @throws \InvalidArgumentException If preAuthToken is not of the good type
 * @throws InvalidPayloadException   If the user identity field is not a key of the payload
 * @throws UserNotFoundException     If no user can be loaded from the given token
 */
public function getUser($preAuthToken, UserProviderInterface $userProvider)
{
    if (!$preAuthToken instanceof PreAuthenticationJWTUserToken) {
        throw new \InvalidArgumentException(
            sprintf('The first argument of the "%s()" method must be an instance of "%s".', __METHOD__, PreAuthenticationJWTUserToken::class)
        );
    }

    $payload = $preAuthToken->getPayload();
    $identityField = $this->jwtManager->getUserIdentityField();

    if (!isset($payload[$identityField])) {
        throw new InvalidPayloadException($identityField);
    }

    $identity = $payload[$identityField];

    try {
        $user = $this->loadUser($userProvider, $payload, $identity);
    } catch (UsernameNotFoundException $e) {
        throw new UserNotFoundException($identityField, $identity);
    }

    $this->preAuthenticationTokenStorage->setToken($preAuthToken);

    return $user;
}

/**
 * {@inheritdoc}
 */
public function onAuthenticationFailure(Request $request, AuthenticationException $authException)
{
    $response = new JWTAuthenticationFailureResponse($authException->getMessageKey());

    if ($authException instanceof ExpiredTokenException) {
        $event = new JWTExpiredEvent($authException, $response);
        $this->dispatcher->dispatch(Events::JWT_EXPIRED, $event);
    } else {
        $event = new JWTInvalidEvent($authException, $response);
        $this->dispatcher->dispatch(Events::JWT_INVALID, $event);
    }

    return $event->getResponse();
}

/**
 * {@inheritdoc}
 */
public function onAuthenticationSuccess(Request $request, TokenInterface $token, $providerKey)
{

    return;
}

/**
 * {@inheritdoc}
 *
 * @return JWTAuthenticationFailureResponse
 */
public function start(Request $request, AuthenticationException $authException = null)
{
    $exception = new MissingTokenException('JWT Token not found', 0, $authException);
    $event = new JWTNotFoundEvent($exception, new JWTAuthenticationFailureResponse($exception->getMessageKey()));

    $this->dispatcher->dispatch(Events::JWT_NOT_FOUND, $event);

    return $event->getResponse();
}

/**
 * {@inheritdoc}
 */
public function checkCredentials($credentials, UserInterface $user)
{
    return true;
}

/**
 * {@inheritdoc}
 *
 * @throws \RuntimeException If there is no pre-authenticated token previously stored
 */
public function createAuthenticatedToken(UserInterface $user, $providerKey)
{
    $preAuthToken = $this->preAuthenticationTokenStorage->getToken();

    if (null === $preAuthToken) {
        throw new \RuntimeException('Unable to return an authenticated token since there is no pre authentication token.');
    }

    $authToken = new JWTUserToken($user->getRoles(), $user, $preAuthToken->getCredentials(), $providerKey);

    $this->dispatcher->dispatch(Events::JWT_AUTHENTICATED, new JWTAuthenticatedEvent($preAuthToken->getPayload(), $authToken));
    $this->preAuthenticationTokenStorage->setToken(null);

    return $authToken;
}

/**
 * {@inheritdoc}
 */
public function supportsRememberMe()
{
    return false;
}

/**
 * Gets the token extractor to be used for retrieving a JWT token in the
 * current request.
 *
 * Override this method for adding/removing extractors to the chain one or
 * returning a different {@link TokenExtractorInterface} implementation.
 *
 * @return TokenExtractorInterface
 */
protected function getTokenExtractor()
{
    return $this->tokenExtractor;
}

/**
 * Loads the user to authenticate.
 *
 * @param UserProviderInterface $userProvider An user provider
 * @param array $payload The token payload
 * @param string $identity The key from which to retrieve the user "username"
 *
 * @return UserInterface
 */
protected function loadUser(UserProviderInterface $userProvider, array $payload, $identity)
{

/*
*Fetch user from database, check if blocked or blacklisted or whatever
 *Return the user or false
 *
*/
}

}
If you want to check the token instead the user do all of the above but the last step and override the getUser method with your custom logic, instead of the loadUser as said.
Hope it helps.

redpajama_set_name: RedPajamaStackExchange


text:
I keep meaning to update my blog with things that I think are interesting or recommendations of Films, music and TV but laziness always gets the better of me. Tonight I'm going to try again with recommendations of two programs that I discovered this year that I would highly recommend to anyone.
Written by Aaron Sorkin, the genius behind The West Wing, Studio 60 on the Sunset Strip and The Social Network. The series takes place in a fictional American Network's news centre. I love the way they work real life news stories into the world of these characters.
It's witty, poignant and sometimes a bit cheesy. It also features the classic Sorkin style of every character being able to talk at 1000 words per minute and rarely being lost for words. But that's something I love about it.
It garnered a lot of criticism from the media when it was released but mainly due to the fact that it was them being scrutinised for a change and being shown what an idealistic version of the news would look like.
Anyway, if you like original, funny and intelligent drama look no further. Hopefully this will get picked up on terrestrial TV at some stage so everyone can enjoy.
Secondly:
Trust me, this show is nothing like the first four episodes of the first season, they are dire, drab and highly forced, but all of a sudden, everything clicks and this show shows it's funny side.
Based in a community college and featuring what look like a clichéd bunch of misfits but are actually very intelligently written and often hilarious characters.
Over it's 3 (and counting) seasons the writers have become more confident in their style and the show has blossomed into one of my favourite comedies of all time.
If you like arrested development, give it a try. It has also suffered a similar treatment at the hands of NBC as they drop it, cut seasons short and move it around schedules as they see fit.
It took me months of convincing Lucy to watch it with me and once we got passed the awkward beginning I can safely say she loves it too and we managed to watch about 30 episodes in one day! (Don't judge, we were having a duvet day!)
One of the standout things for me are the multiple references to pop culture such as the Goodfellas style episode and the now legendary paintball episodes directed by Fast & Furious director Justin Lin.
Now if only NBC don't cancel it…..SixSeasonsAndAMovie.
December 13, 2012 December 13, 2012 haveyoumetmattCommunity, recommendations, The Newsroom
Previous Previous post: The Boss! Music and other interests….
Next Next post: My Favourite Films 2012


A small, curious kitty trapped in the wall of a home will get a chance to live out its eight other lives thanks to a Palm Bay Fire Rescue crew.
Firefighters were called out to a home Sunday in the 300 block of Meadowbrook Road in the northeast section of the city. The homeowner told firefighters his cat had somehow gotten caught behind a wall in the kitchen and could hear him meowing.
"The kitty was walking across the upper kitchen cabinets and fell into a void area that wasn't accessible," Palm Bay Fire Rescue Battalion Chief Gaius Hall said.
Firefighters arrived within minutes and set up equipment as the trapped kitten continued to purr behind the wall of the kitchen.
"The area was only accessible by cutting the drywall. The firefighters had to figure out the stud pattern of the wall before cutting. But they were able to reach the cat," Hall said. Once a portion of the wall was removed, firefighters reached inside to free the feline.
Hall said the cat was roaming inside when the incident happened. "Cats being cats, they do weird stuff," Hall said.


In years past "only" 2 weeks on the market wouldn't have meant anything….that hasn't been the case over the past two years with homes in Fusion often going into escrow in the first week and some selling before they even hit the market. The average days on market for the homes that sold in Fusion in 2018 was 11. With one home currently for sale and on the market for 15 days and another on the market for over three weeks, does that indicate a slight slowdown or just a temporary blip due to the time of year and weather? This is normally a slower time of year so we'll know more as to whether the market heats up along with the weather.
As of 1/31 there are two active listings in Fusion. The first is a "C" floorplan at 5406 W 149th Pl # 18 that is priced at $735,000 and the other an "E" floorplan at 5403 W 149th Place Unit#4 currently priced at $799,000. E floorplans had been one of the highest demand layouts over the past 2 years.
Want and easier way of keeping current with new listings. Tell us just a little about your home search and received free email listing alerts when a new home hits the market in Fusion.


From September 4 to 15, 2017 social consultations with the citizens of Kamionka district will be held. Consultations refer to adopting a statute of the district.
Consultations will be run online – a form to be downloaded at http://bip.mikolow.eu/index.php/dokumenty/9600 and in paper – forms to be taken and cased at the Town Office.
Kindly invite you to voting.


text:
Bagrat of Tao may refer to:

 Bagrat I of Tao (died 945), Georgian prince and son of Adarnase IV
 Bagrat II of Tao (died 966), Georgian prince and son of Adarnase II Kuropalates


We're big fans of the guys over at YM360 and thankful for their continued support of the Download Youth Ministry Show. Youth Ministry 360 has a great Christmas freebie vault you should check out for great small group stuff, lessons and more Christmas goodness. Check them out!


1" x 30' (thirty foot) rolls of our Reflexite V92 - 5 year rated DOT conspicuity tape available in the popular 7 inch white by 11 inch red pattern, a 6" red by 6" white pattern or in solid white.
This is an extremely bright commercial grade tape. The brightness and the contrast between the two colors is what makes this tape stand out day and night.


text:
Self-Consciousness in Its Relation to Character
by F.U.
This is a self-conscious age. In all walks of life; in politics, in literature, even in philanthropy, the age is subjective. Education in its various branches tends to the development of individualism, and the tendency of individualism is to be conscious of self. This is, however, to a great extent the inevitable attribute of an active condition. It is very beautiful to see an entire absence of self-consciousness as is usually the case in little children, but when not spoilt by over-indulgence, or worse by over-severity, but when the child is a little older, and awakes to its relations with things outside itself and immediate surroundings, the self-conscious age is reached, and is shown usually by sudden shyness and apparent stupidity, or by the rooted conviction in the child that he knows better and more on every possible subject than his elders. Trying though this last phase certainly is, there is still so much in it of promise, so much evidence of existing character, that friends and relations may well wait with patience, and hope for self-consciousness to give place to self-possession-that true possession of the soul, which Matthew Arnold saw regretfully was so often missed in the hurry and bustle of life-
"We see all sights from pole to pole
And glance, and nod, and bustle by
And never once possess our soul
Before we die."
The absence of self-consciousness, so beautiful wherever met with, is then we see usually characteristic of childhood, [An instance of painfully precocious self-consciousness in childhood may be found in the "Life of the late Lord Lytton," who, when very young, startled his mother enquiring if she were conscious of her own identity!] while its existence is often a product of growth and education. Is it then to be termed a fault of the age, or is it not rather a sign of progress? To both of these questions I think the answer should be in the affirmative. Self-consciousness is undoubtedly a faulty condition, but it may also belong to a transitional stage of real progress.
Among savage tribes it is entirely absent; its development is unmistakably a product of civilization, and like most other such products has both a good and a bad side. To realize oneself, to be conscious of one's own individuality is a step towards further growth, but to stop there would be at once to put a limit to growth. An infant, when it begins to awaken to the consciousness of innate powers, endeavours at once to put them to the test. It becomes, for example, conscious of powers which shall enable him to speak and to walk, and this realization leads to crowing and kicking-the first advance towards speaking and walking. It is the same with us in sleep-in deep sleep at any rate-self is lost in unconsciousness, but with our waking comes again the consciousness of self, and with the consciousness the call to action. The realization of self is the first step towards greatness, but to become greatness it must pass rapidly over the self-conscious stage, and translate being and doing, and to do this involves conquest of that very self which has cost at first so much to develop.
"Self-reverence, self-knowledge, self-control,
These three alone lead life to Sovereign power,"
Says Tennyson in "CEnone," and this is the whole secret. The child conscious of the power of speech has to learn to speak, and that done must learn to control speech. The man who has learned the great and awful truth of his own individuality, must first learn the way in which he can use it, and then how to conquer it. "Resolve," says Matthew Arnold,
"To be thyself, and know that he
Who finds himself, loses his misery,"
but this is only a half-truth, for to truly find oneself, one must also lose oneself. Life is full of paradoxes, and being one's best self, is the result alike of self-development and of self-repression. It is interesting in this connection to compare two great modern poets--Browning and Tennyson. Browning's note is always one of aspiration.
"A man's reach should exceed his grasp,
Aspire to be, to develop what is in you. This is his message to man.
Tennyson on the other hand bids men struggle to repress evil that good may grow,
"Acting the law we live by without fear,"
but both alike point to men finding their true being ultimately in loving, and it is in loving truly and really that self-consciousness must inevitably die, though the love may perhaps be deeper and stronger for that previous self-knowledge of which we have been speaking. To again quote words of Tennyson's--
"Love took up the harp of Life, and smote on all the chords with might,
Smote the chord of Self, that trembling, pass'd in music out of sight."
And certain it is that unless self-consciousness can evaporate in some such way as that, it will become that deadliest of moral foes, Egotism. In many cases self-consciousness is of course, merely a form of Egotism, pure and simple, while in others, as shown in the case of the strange Autobiography of Marie Bashkirtseff, it may become the acme of morbid sensibility, but we are considering it here more as that sense of individuality which truly may grow into Egotism or morbid sensitiveness, but which may also ripen into self-conquest, and in speaking of the characteristics of the age in which we live, we may, I think, say thankfully, that Egotism pre se is less a fault characteristic of our nation now than ever before. Men are more ready to-day to give themselves, and to sacrifice themselves for the good of others and the welfare of their country, but they are conscious of what it is they are doing. The old abandon of personal interests which led men silently to go forth to death or victory in the early and middle ages, has given place to a higher-because more personal-spirit of self-sacrifice, but takes generally (individually there are, of course, very many nobles exceptions), there is less silence about what is being done. Men are waking more than ever before to the realization of power in and around them, and above all to the recognition of a common brotherhood-which is, in truth, the product of the highest individualism-but they talk about what they feel, and think, and do, as they did not previously. It is an age of experiments, of systems, of theories; educational, political, and social discussions are the very air we breathe, but in all these there is life. Like the school boy, the world thinks it knows nearly everything, and applies itself to exercise its knowledge on the great questions of life. And rightly so. But like the school boy, it is, perhaps, only passing through a phase which may be the passport to fuller knowledge.
Self-consciousness then, if it be a fault of the age in which we live, is still, if we can use the antithesis, a good fault, for it bears in it the seeds of life and of progress, which may in time blossom into a noble self-forgetfulness, and at the same time into a truer "possessing" of our souls than ever before. With this belief we may well regard with patience and with hope its often presence in the young, recognizing it as an earnest of possible strength and nobility of character in the future.
F.U.