text:
A novel CASR mutation ...
A novel CASR mutation (p.Glu757Lys) causing autosomal dominant hypocalcaemia type 1
in Endocrinology, Diabetes & Metabolism Case Reports
Authors: Benjamin Kwan 1 , 2 , Bernard Champion 1 , 3 , Steven Boyages 1 , 4 , Craig F Munns 1 , 5 , Roderick Clifton-Bligh 1 , 6 , Catherine Luxford 1 , 6 and Bronwyn Crawford 1 , 2
1 University of Sydney, Sydney, New South Wales, Australia
2 Department of Endocrinology, Concord Repatriation General Hospital, Sydney, New South Wales, Australia
3 Department of Clinical Medicine, Macquarie University, Sydney, New South Wales, Australia
4 Department of Endocrinology, Westmead Hospital, Sydney, New South Wales, Australia
5 The Children's Hospital at Westmead, Sydney, New South Wales, Australia
6 Cancer Genetics Laboratory, Kolling Institute, Royal North Shore Hospital, Sydney, New South Wales, Australia
https://doi.org/10.1530/EDM-18-0107
Volume 2018: Issue 1
Abstract/Excerpt
Autosomal dominant hypocalcaemia type 1 (ADH1) is a rare familial disorder characterised by low serum calcium and low or inappropriately normal serum PTH. It is caused by activating CASR mutations, which produces a left-shift in the set point for extracellular calcium. We describe an Australian family with a novel heterozygous missense mutation in CASR causing ADH1. Mild neuromuscular symptoms (paraesthesia, carpopedal spasm) were present in most affected individuals and required treatment with calcium and calcitriol. Basal ganglia calcification was present in three out of four affected family members. This case highlights the importance of correctly identifying genetic causes of hypocalcaemia to allow for proper management and screening of family members.
ADH1 is a rare cause of hypoparathyroidism due to activating CASR mutations and is the mirror image of familial hypocalciuric hypercalcaemia.
In patients with ADH1, symptoms of hypocalcaemia may be mild or absent. Basal ganglia calcification may be present in over a third of patients.
CASR mutation analysis is required for diagnostic confirmation and to facilitate proper management, screening and genetic counselling of affected family members.
Treatment with calcium and activated vitamin D analogues should be reserved for symptomatic individuals due to the risk of exacerbating hypercalciuria and its associated complications.
ADH1 is a rare disorder arising from activating mutations of the calcium-sensing receptor gene (CASR). It is the mirror image of familial hypocalciuric hypercalcaemia and is characterised by hypocalcaemia with low or inappropriately normal PTH. To date, more than 70 mutations have been reported in association with ADH1 (1). Treatment with activated vitamin D analogues and calcium should be reserved for symptomatic patients, due to the risk of hypercalciuria and severe complications such as nephrocalcinosis, nephrolithiasis and renal impairment. We describe an Australian family with a novel heterozygous missense mutation causing ADH1.
A 40-year-old Caucasian woman presented with muscle spasms affecting the hands, feet, abdomen and chest. She had been diagnosed with hypocalcaemia at age 23 years during her second pregnancy. At that time, she was noted to have basal ganglia calcification on CT imaging performed for investigation of Bell's palsy. She had a history of childhood petit mal seizures. Her medications included calcitriol 0.25 µg daily, calcium 600 mg daily and cholecalciferol 1000 IU daily. Despite this, she had symptomatic hypocalcaemia with an inappropriately low-normal PTH: serum calcium 1.94 mmol/L (reference range 2.15–2.55 mmol/L), PTH 1.7 pmol/L (reference range 1.6–6.9 mmol/L). Serum magnesium, phosphate and creatinine levels were in the normal range. A 24-h urine calcium while on treatment was 7.2 mmol/day (reference range 2.5–7.5 mmol/day).
There was a family history of hypocalcaemia also in her brother, nephew and father (Fig. 1). Her 43-year-old brother had a history of recurrent muscle spasms since youth. After being diagnosed with type 1 diabetes mellitus at age 28 years, he decided to undergo a whole-body CT scan (as part of a general health check), which showed basal ganglia calcification. Serum calcium was low (1.93 mmol/L), as was PTH (0.7 pmol/L). He was subsequently treated with oral calcium, calcitriol 0.5 µg bd and magnesium. In 2007, when his 3-year-old son required a blood test for an unrelated, acute febrile illness, his father requested serum calcium be measured. The serum calcium was 1.82 mmol/L, PTH 1.0 pmol/L, phosphate 2.25 mmol/L (reference range 0.80–1.50), with normal serum creatinine and magnesium. A spot urine calcium to creatinine ratio was 0.26 (reference range 0.05–1.1). There was no calcification seen on a brain CT at age 4 years. During childhood, he developed muscle cramps at night after prolonged activity and episodes of jaw tetany. He also had a history of attention deficit hyperactivity disorder and obsessive compulsive disorder. There was no history of seizure disorder. He was treated with calcitriol 0.75 µg bd and calcium 600 mg daily, with symptomatic relief and maintenance of serum calcium between 1.8–1.9 mmol/L. Serial renal tract ultrasounds have not demonstrated nephrolithiasis.
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Family pedigree. The arrow indicates the proband. Filled symbols, individuals with hypocalcaemia; dotted symbols, status unknown; slash, deceased; E, CASR gene mutation analysis.
Citation: Endocrinology, Diabetes & Metabolism Case Reports 2018, 1; 10.1530/EDM-18-0107
The index patient's 65-year-old father had asymptomatic hypocalcaemia discovered incidentally after he presented with an electrocution injury many years ago. He had no symptoms of hypocalcaemia. He had a history of type 2 diabetes mellitus, chronic obstructive pulmonary disease, vitamin B12 deficiency and gastroesophageal reflux disease. His serum calcium was 1.92 mmol/L, PTH 2.7 pmol/L and 24-h urine calcium 1.9 mmol/day. A brain MRI showed bilateral basal ganglia and cerebellar dentate nuclei calcification. He was not treated with any calcium or calcitriol.
The index patient underwent genetic testing. Chromosomal analysis with fluorescence in situ hybridisation was arranged primarily to exclude 22q11.2 deletion syndrome (DiGeorge syndrome) and was normal. Sanger sequencing revealed a heterozygous CASR missense variant (c.2269G>A, p.Glu757Lys). The affected family members (father, II-1; brother, III-2; nephew, IV-1 – see Fig. 1) were subsequently referred for CASR mutation analysis and each found to harbour the same CASR variant (c.2269G>A, p.Glu757Lys) (Table 1). This variant has not yet been reported to be associated with ADH1 and has not been identified in normal population cohorts. Its pathogenicity is supported by segregation with phenotype in our kindred, its absence from population cohorts and in silico prediction tools (Polyphen-2, MutationTaster) (2, 3).
Characteristics of affected individuals.
Pedigree numbera
Age, years
Serum calcium (corrected), mmol/Lb
PTH, pmol/Lc
Basal ganglia calcification
CASR gene analysis
Proband III-1 F 40 1.93 1.7 Calcitriol, calcium Yes Muscle spasmsChildhood petit mal seizures Heterozygous c.2269G>A
Father II-1 M 65 1.92 2.7 None Yes Asymptomatic Heterozygous c.2269G>A
Brother III-2 M 43 1.93 0.7 Calcitriol, calcium Yes Muscle spasms Heterozygous c.2269G>A
Nephew IV-1 M 13 1.78 1.0 Calcitriol, calcium No Muscle spasmsFatigue Heterozygous c.2269G>A
aPedigree number as appears on family pedigree in Table 1. bSerum calcium reference range, 2.15–2.55 mmol/L. cPTH reference range 1.6–6.9 mmol/L.
Outcome and follow-up
To date, there have been no adverse effects of treatment, such as nephrolithiasis, nephrocalcinosis or renal impairment in affected individuals treated with calcium and calcitriol. They are monitored with annual biochemistry (serum calcium, phosphate, creatinine), annual 24-h urine calcium measurement and second yearly renal tract ultrasound.
All affected individuals received genetic counselling. Laboratory and genetic testing was offered to the index patient's asymptomatic sister, who has declined. Our index patient's four offspring and her brother's unaffected offspring are currently asymptomatic (see Fig. 1). Serum calcium will be measured opportunistically whenever they should require laboratory testing for other reasons.
We describe three generations of a family with ADH1 due to a novel heterozygous CASR missense mutation (c.2269G>A, p.Glu757Lys). ADH1 is a rare disorder arising from activating CASR mutations, characterised by hypocalcaemia and hypoparathyroidism due to a left-shift in the set point for extracellular calcium (4). Most reported mutations causing ADH1 are heterozygous missense mutations (www.casrdb.mcgill.ca) (1). Relative hypercalciuria may occur due to reduced PTH and lower renal calcium reabsorption, as well as increased activation of the mutated CASR in the distal renal tubule (5). The clinical presentation tends to be variable, ranging from asymptomatic hypocalcaemia to mild neuromuscular symptoms, and in severe cases, may manifest as seizures (6). Basal ganglia calcification is seen commonly in ADH1. In one series, 36% of patients were observed to have basal ganglia calcification, with the youngest patient being 5 years of age (7). In our cohort, several kindred had basal ganglia calcification seen at diagnosis, consistent with this being a complication of the disease rather than its treatment.
Accurate diagnosis of ADH1 with confirmatory genetic testing is important to allow for appropriate screening and genetic counselling of family members and to avoid unnecessary treatment. Treatment of asymptomatic individuals should be avoided due to the risk of exacerbating hypercalciuria and its associated complications (nephrocalcinosis, nephrolithiasis and renal impairment). In symptomatic individuals, the aim of therapy should be to achieve symptom relief using the lowest possible dose of calcium and activated vitamin D analogues rather than aiming to achieve normocalcaemia. Thiazide diuretics may be used alongside calcitriol to reduce renal calcium excretion.
This case reports a novel CASR mutation causing ADH1. In patients presenting with hypoparathyroidism, a positive family history of hypocalcaemia should prompt clinicians to consider rarer familial causes, as these diagnoses will have important implications on management, screening and genetic counselling of affected individuals.
The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of this case report.
This research did not receive any specific grant from any funding agency in the public, commercial or not-for-profit sector.
All data have been fully anonymised and there is no identifying information in this manuscript. As such, written informed consent was not obtained but can be pursued at the journal's request.
Author contribution statement
B Kwan reviewed the literature and prepared the manuscript. B Champion, S Boyages and C Munns were treating physicians and reviewed the manuscript. R Clifton-Bligh and C Luxford assisted in reviewing the manuscript. B Crawford was the treating physician for the index patient and oversaw the preparation of the manuscript.
PidashevaSD'Souza-LiLCanaffLColeDEHendyGN. CASRdb: calcium-sensing receptor locus-specific database for mutations causing familial (benign) hypocalciuric hypercalcemia, neonatal severe hyperparathyroidism, and autosomal dominant hypocalcemia. Human Mutation 2004 24 107–111. (https://doi.org/10.1002/humu.20067)
PidashevaSD'Souza-LiLCanaffLColeDEHendyGN. CASRdb: calcium-sensing receptor locus-specific database for mutations causing familial (benign) hypocalciuric hypercalcemia, neonatal severe hyperparathyroidism, and autosomal dominant hypocalcemia. Human Mutation 2004 24 107–111. (https://doi.org/10.1002/humu.20067)1524179110.1002/humu.20067)| false
AdzhubeiIASchmidtSPeshkinLRamenskyVEGerasimovaABorkPKondrashovASSunyaevSR. A method and server for predicting damaging missense mutations. Nature Methods 2010 7 248–249. (https://doi.org/10.1038/nmeth0410-248)
AdzhubeiIASchmidtSPeshkinLRamenskyVEGerasimovaABorkPKondrashovASSunyaevSR. A method and server for predicting damaging missense mutations. Nature Methods 2010 7 248–249. (https://doi.org/10.1038/nmeth0410-248)10.1038/nmeth0410-24820354512)| false
SchwarzJMCooperDNSchuelkeMSeelowD. MutationTaster2: mutation prediction for the deep-sequencing age. Nature Methods 2014 11 361–362. (https://doi.org/10.1038/nmeth.2890)
SchwarzJMCooperDNSchuelkeMSeelowD. MutationTaster2: mutation prediction for the deep-sequencing age. Nature Methods 2014 11 361–362. (https://doi.org/10.1038/nmeth.2890)2468172110.1038/nmeth.2890)| false
ConigraveAD. The calcium-sensing receptor and the parathyroid: past, present, future. Frontiers in Physiology 2016 7 563. (https://doi.org/10.3389/fphys.2016.00563)
ConigraveAD. The calcium-sensing receptor and the parathyroid: past, present, future. Frontiers in Physiology 2016 7 563. (https://doi.org/10.3389/fphys.2016.00563)28018229)| false
RoszkoKLBiRDMannstadtM. Autosomal dominant hypocalcemia (hypoparathyroidism) types 1 and 2. Frontiers in Physiology 2016 7 458. (https://doi.org/10.3389/fphys.2016.00458)
RoszkoKLBiRDMannstadtM. Autosomal dominant hypocalcemia (hypoparathyroidism) types 1 and 2. Frontiers in Physiology 2016 7 458. (https://doi.org/10.3389/fphys.2016.00458)27803672)| false
TanYMCardinalJFranksAHMunHCLewisNHarrisLBPrinsJBConigraveAD. Autosomal dominant hypocalcemia: a novel activating mutation (E604K) in the cysteine-rich domain of the calcium-sensing receptor. Journal of Clinical Endocrinology and Metabolism 2003 88 605–610. (https://doi.org/10.1210/jc.2002-020081)
TanYMCardinalJFranksAHMunHCLewisNHarrisLBPrinsJBConigraveAD. Autosomal dominant hypocalcemia: a novel activating mutation (E604K) in the cysteine-rich domain of the calcium-sensing receptor. Journal of Clinical Endocrinology and Metabolism 2003 88 605–610. (https://doi.org/10.1210/jc.2002-020081)10.1210/jc.2002-020081)| false
RaueFPichlJDörrHGSchnabelDHeidemannPHammersenGJaursch-HanckeCSantenRSchöflCWabitschMet al. Activating mutations in the calcium-sensing receptor: genetic and clinical spectrum in 25 patients with autosomal dominant hypocalcaemia - a German survey. Clinical Endocrinology 2011 75 760–765. (https://doi.org/10.1111/j.1365-2265.2011.04142.x)
RaueFPichlJDörrHGSchnabelDHeidemannPHammersenGJaursch-HanckeCSantenRSchöflCWabitschM, et al. Activating mutations in the calcium-sensing receptor: genetic and clinical spectrum in 25 patients with autosomal dominant hypocalcaemia - a German survey. Clinical Endocrinology 2011 75 760–765. (https://doi.org/10.1111/j.1365-2265.2011.04142.x)2164502510.1111/j.1365-2265.2011.04142.x)| false
Gland/Organ
Parathyroid
Genetics and mutation
Condition/ Syndrome
Hypocalcaemia
Country of Treatment
Paraesthesia
Calcium (serum)
Calcium (urine)
Calcium to creatinine clearance ratio
Creatinine (serum)
Molecular genetic analysis
Phosphate (serum)
Calcitriol
Cholecalciferol
Case Report Type
New disease or syndrome: presentations/diagnosis/management
This is an Open Access article distributed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. 2018
ELocation ID:
Received Date:
Accepted Date:
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PubMed Citation
Article by Benjamin Kwan
Article by Bernard Champion
Article by Steven Boyages
Article by Craig F Munns
Article by Roderick Clifton-Bligh
Article by Catherine Luxford
Article by Bronwyn Crawford


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New market insight from Mintel identified key ingredient and food and beverage product trends that will influence the global market over the next year. 2017 will be a "year of extremes"—from "ancient" products including grains, recipes, practices and traditions to the use of technology to create more and better tasting plant-enhanced foods.
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In Tradition We Trust: Consumers seek comfort from modernized updates of age-old formulations, flavors and formats. The trust in the familiar emphasizes the opportunity for manufacturers to look to the past as a dependable source of inspiration such as "ancient" product claims including ancient grains and also ancient recipes, practices and traditions.
Power to the Plants: The preference for natural, simple and flexible diets will drive further expansion of vegetarian, vegan and other plant-focused formulations. More packaged products and recipes for home cooking will leverage fruits, vegetables, nuts, seeds, grains, botanicals and other plants as a way to align with consumers' nearly omnipresent health and wellness priorities.
Time is of the Essence: The time investments required for products and meals will become as influential as nutrition or ingredient claims. Time is an increasingly precious resource and our multitasking lifestyles are propelling a need for shortcut solutions that are still fresh, nutritious, and customizable. In 2017, the time spent on—or saved by—a food or drink product will become a clear selling point, inspiring more products to directly communicate how long they will take to receive, prepare or consume.
The Night Shift: The increasingly hectic pace of modern life is creating a market for food and drink that helps people of all ages calm down before bedtime, sleep better, and restore the body while they rest. There is potential for more evening-focused innovations formulated for relaxation, satiety, and, taking a cue from the beauty industry, food and beverages that provide functional benefits while the consumer sleeps.
Balancing the Scales—Health for Everyone: Healthy food and drink are not "luxuries." Many lower-income consumers want to improve their diets but the access to—and the cost of—healthy food and drink is often an impediment. More campaigns and innovations are to be expected that will make it easier for lower-income consumers to fulfill their healthy ambitions, including apps to help people make use of ingredients that are on sale.


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Partners for Better Futures
Build A School In Burma
Myanmar Humanitarian Relief Fund
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APC School
APC school - Philippines
About APC:
The Apu Palamguwan Cultural Education Center (APC) is an indigenous-people-based educational institution located in the village of Sitio Bendum. In 2004, the Department of Education formally recognized and granted APC a permit to operate as an upland elementary school for all children.
The mission of APC is to provide an integrated, holistic, and culture-based education for the indigenous children and youth along the Pantaron Range. We achieve this by promoting and deepening the children's understanding of their unique culture, while providing knowledge and skills that enable them to confidently relate with mainstream Philippine society. Given this foundation laid in the community, there is hope that the cultural life of the Pulangiyen who are dedicated to restoring and sustaining their forest, water, biodiversity and their agricultural resources, in all their diversity, will thrive.
The APC Program Directors:
Shirley and Mark Kirchen have been retired since 1999, traveling to places like Beijing, Bangkok, and Anchorage volunteering as teachers of English as a second language and in GED programs for those seeking a high school diploma, as well as serving in a soup kitchen and food pantry when home in the San Francisco Bay Area. In 2010 they met Fr. Pedro Walpole, S.J. at the home of Shirley's sister in Manila.
Per Mark and Shirley Kirchen:
"We were very impressed with what we heard about the village school Pedro had founded at the village of Bendum in the hill country of Mindanao, so we accepted his invitation to visit the APC school. The unique program of this school is the most effective we have seen for sustaining indigenous language and cultural values, and has now become the main focus of our volunteer efforts and will remain so. We visited the school again in April, 2016 and witnessed significant growth: from a single grade school to the addition of four connecting schools, K through third grade (in temporary facilities) and a new intergrated high school (in a permanent facility); from room and board for 20 students from neighboring villages to the same support for 80. The combined schools support more than 300 children at $1 per day each.
The curriculum is integrated with the cultural traditions of this forest people, preserving their Pulangiyen language and indigenous culture of forest preservation and self-sustaining agriculture. It also addresses engagement with the world at large through the acquisition of speaking, reading, and writing skills in Visayan, the regional language, Filipino, the national language, and English, the international language. The more than $40,000 per year contributed through our efforts, when added to the community's own self support efforts and other contributors, has been a vital resource for the ongoing operation of the school. We remain committed to matching all contributions dollar for dollar in the hope of sustaining a vital community of interest for this important program."
APC Leadership:
The partnership with the Pulangiyen people began in 1992, when the community of Sitio Bendum sought the help of Father Pedro Walpole, SJ to set up a school. With Pedro leading, the Environmental Science for Social Change (ESSC) assisted the community in setting up the Cultural Empowerment Center in 1992. Young volunteers from the Year of Service (YOS) and Jesuit Volunteer (JVP) Programs began to organize literacy classes with help from community members. They developed a full-fledged elementary curriculum teaching the local culture, with the mother tongue as a subject and as the language of instruction in the lower grades. Cultural knowledge and local community concerns were also incorporated in the curriculum. In later grades three more languages are added to provide contact with national and international concerns. There are now four local village schools connected to the main campus, and the first three years of high school are in operation. The school draws on local residents for both teachers and administrators. This is truly a community based and supported school.
Donating to APC:
The Apu Palamguwan Cultural Education Center (APC School) on Mindanao in the Philippines provides education in their indigenous culture to over 400 students in grades K through 12. The goal of the school is to put these forest dwellers in control of the future of their own culture. The children rely on support from those who know about them and care for them. You are able to sustain the education and welfare of a student at the rate of $1 per day, and we who sponsor this effort will match your contribution dollar for dollar. Thus your $30 results in support for two students for a month, while a donation of $350 does so for two children for an entire year. Any amount yields disproportionate results and is deeply appreciated by these children.
All contributions are tax deductible and are matched. They can be made by check or credit card. Checks avoid a 5% bank processing fee and should be made payable to: APC School – Philippines.
Donations by check can be mailed to :
1275 Fairway Drive
Your gift will support holistic education for indigenous children in Mindanao,Philippines.
Online donation to APC School
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UPS Worldwide Express expands to 14 new countries
Nov 13, 2018 | E-Commerce, News, Parcel | 0
Fourteen new countries are now being served by UPS Worldwide Express, part of UPS's international suite of shipping services that guarantees time- and day-definite delivery for urgent shippers.
The expansion of global services also includes later pick-up times, improved time-in-transit and Saturday delivery in seven countries in ISMEA, all part of the company's desire to better connect businesses to its smart global logistics network, while demonstrating the company's strategic focus on international high-growth markets.
"We're excited to announce that customers can now choose UPS to better compete globally with the time-definite services competitive businesses need," said Nando Cesarone, president, UPS International. "By improving time-in-transit and increasing the number of countries served, our customers have more time-of-day delivery options to better meet their customers' critical needs."
The guaranteed time- and day-definite delivery improvements include South and Central America, including Panama where customers can benefit from the Colón Free Trade Zone, plus Europe, the Middle East and Africa, including Nigeria, the largest economy in Africa by GDP and population.
UPS also grew its express services footprint within key markets by adding new postal codes in 26 European countries, as well as in other high-growth markets such as South Korea, China, Hong Kong, Indonesia and Singapore, all of which have exceeded annual per capita GDP growth of 3.5% for the last 50 years.
"In addition to expanding its service area, UPS improved time in transit to 24 countries, primarily emerging markets, thereby increasing customers' speed to market," said Jean-Francois Condamine, UPS president of Growth and Emerging Markets. "This includes India, where UPS recently announced an investment to consolidate and strengthen its position by attaining full ownership in its express services in one of the world's fastest growing economies."
For customers with urgent freight needs, UPS Worldwide Express Freight Midday now offers time-definite delivery of international palletized shipments to over 39,000 new postal codes in Europe. The additional postal codes increases the footprint of this guaranteed service by five times, enabling shipments to over 35 countries and territories and the ability to receive packages from over 70 countries and territories.
"UPS wants to give its customers more options to deliver faster, earlier, on the weekend, and with later pick-up and cut-off times," added Cesarone. "We provide importer and exporters with greater flexibility and guaranteed services to fit their time-sensitive needs around the world."
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The Telecom Ministry is accepted to have looked for elucidation from Ringing Bells for promoting its "Freedom251" mobile phone without BIS confirmation and have requested that Uttar Pradesh government check its certifications, according to BJP MP Kirit Somaiya.
Kirit Somaiya has said that there is a huge scam involved in 'Flexibility 251'. After going through all papers of the organization, he discovered that the 'Freedom 251' doesn't have a permit.
This is a huge scam, that is the reason I experienced every one of the papers of the company. The government has informed Uttar Pradesh government to check the bonafide of the promoter. This is a Ponzi bogus company scam," Somaiya told.
The BJP MP has approached telecom minister, telecom operator TRAI, consumer ministry, Sebi, corporate service, account service, RBI and state governments to check different concerns that he has on the company offering the mobile phone for Rs 251– which is demonstrating the world's cheapest cell phone.
He also scrutinized the foundation, the money related status, the owners of Ringing Bells Pvt Ltd, saying that he was "a long way from persuaded" with the reaction given by Ashok Chadha, President of Ringing Bells. Chadha, in the guard of his company and product, had said that cost was conveyed down because of local amassing and online marketing.
Bollywood super star Shah Rukh Khan (SRK) in the last couple of days, the state police of Gujarat decided to strengthen the security spread on the arrangements of his up coming film 'Raees'. Senior officials said that particular region and city police chief have been ordered that reviewed the situation and give satisfactory security spread at lodgings and spots of shooting.
The expanded security spread was given when the group of the film went Adalaj stepwell for shooting. Gandhinagar locale police had sent more than 150 personnal, including a deputy superintend of police, two reviewers and 10 sub-inspectors.
"Security of the actor and the film group has been checked on and police organization has been expanded for the future occurrences too," said Shivanand Jha, city police official.
SRK has confronted protest by Vishwa Hindu Parishad (VHP) at Bhuj and Ahmedabad. From the get-go morning, bike borne young people had pelted stones at the hotels where the actor and film group were staying in which an car using by unit had got harm. A group of BJP Yuva Morcha had also tried to burn representation of the actor outside the hotel before which the group was arrested by Vadaj police.


The Police in Enugu State have arrested a 21-year-old student for allegedly killing her baby soon after delivery.
The spokesman for the Police Command in Enugu State, SP Ebere Amaraizu, in a statement on Sunday said the suspect (name withheld) allegedly carried out the action at Royal Lodge, Agbani, Nkanu West Local Government Area, on Saturday.
"It was gathered that the mother of the late infant was said to have given birth to the baby on November 17 and allegedly killed the child immediately after birth inside her room before the arrival of neighbours.
"It was further gathered that the cry of a baby was heard in the suspect's room, which attracted neighbours to her room, but they could not gain access until they forced themselves in.
"The neighbours only discovered after gaining access into the room that the baby that was delivered had been allegedly killed by the mother,'' he said.
"The dead body of the baby was also recovered by the policemen,'' he said.


text:
TV Lies
Series: Turn On The News
Subtitle: Weekly newscast from the fictional Radio Network, with parody radio coverage of the radio and its headlines.
Featured Speakers/Commentators: Songs and works from Husker Du ("Turn On the News"), Big Star ("Don't Lie to Me"), Billy Joel ("Honesty"), The Castaways ("Liar, Liar"), and The Disposable Heroes of Hiphoprisy.
Contributor: Wave Farm/WGXC 90.7-FM [Contact Contributor]
License: Attribution Non-commercial Share Alike (by-nc-sa)
Summary: "Turn On The News" is the weekly newscast from the fictional Radio Network, with parody radio coverage of the radio and its headlines. Now with computerized news readers, and fewer meddling reporters, plus aggregated reporting, and automated music. Tune in "Turn On The News" each week for the latest news, radio art, and more from our robot reporters, making sure you hear both sides -- good and evil -- every time you "Turn On The News."
Credits: Clips and excerpts from Seth Meyers; Rachel Maddow; Donald Drumpf; Abe Simpson; Jen Psaki; Ron Johnson; Tucker Carlson; and Nicolle Wallace.
Notes: "Turn On The News" is the weekly newscast from the fictional Radio Network, with parody radio coverage of the radio and its headlines. Now with computerized news readers, and fewer meddling reporters, plus aggregated reporting, and automated music. Tune in "Turn On The News" each week for the latest news, radio art, and more from our robot reporters, making sure you hear both sides -- good and evil -- every time you "Turn On The News." This week: "TV Lies." This week there were weighty issues on trial, and a verdict came in. Songs and works from Husker Du ("Turn On the News"), Big Star ("Don't Lie to Me"), Billy Joel ("Honesty"), The Castaways ("Liar, Liar"), and The Disposable Heroes of Hiphoprisy ("Television The Drug Of The Nation"). Clips and excerpts from Seth Meyers; Rachel Maddow; Donald Drumpf; Abe Simpson; Jen Psaki; Ron Johnson; Tucker Carlson; and Nicolle Wallace. Episode 014.
Version 1: TV Lies
Description: Weekly newscast from the fictional Radio Network, with parody radio coverage of the radio and its headlines.
Location Recorded: Recorded at Wave Farm in the Hudson Valley in New York.
1 turnonnews014 00:28:00 128Kbps mp3


The MAT is given online in the Testing Center by appointment only. Call the Testing Center at (580)581-2502 to make an appointment, at least seven days prior to your desired test date.
On the day of the exam, arrive at the Testing Center, fifth floor, North Shepler, at least ten minutes before your scheduled exam. The fee is $65.00 and is to be paid with a check or money order made payable to Cameron University. Two forms of I.D. are required, one must be a photo I.D.. Acceptable form of a photo I.D. include a driver's license, military I.D., or current passport.


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An 11 a.m. funeral service for Patricia Ann Thomas, aka Big Red, 82, of Albany, Oregon, formerly of Spirit Lake, will be held at the Turner Jenness Funeral Home in Spirit Lake on Thursday, March 14, 2019, with the visitation beginning at 10 a.m.
Patricia Ann Thomas, the daughter of Rubin C. White and Jewel Edna (Doyel) White was born Sept. 11, 1936, in Marianna, Arkansas. After graduating from Marianna High School, Patricia attended Memphis State.
On Sept. 8, 1959, Patricia was married to Christopher Mark Thomas. While Patricia lived in Miami, she spent several years working for Eastern Airlines as a Flight Attendant and later as a reservations agent. After moving to Iowa in 1970, she served her community for several decades as a Realtor in the Iowa Great Lakes region. In 2013, Patricia moved to Oregon to live with her daughter and family.
Patricia loved her children and the children of Spirit Lake. She spent several years as a school bus driver making sure that children had a safe ride to school and back again. She often drove the bus to her children's events, ensuring that she would get to attend their games and tried to attend as many of their events as possible.
Patricia loved her flower and vegetable gardens. She spent her summers tending her gardens only to give much of it away to her cherished family, neighbors and friends. Sharing the fruits of her labor with others gave her great joy. She kept a sandbox and swing set in her backyard long after her grandchildren had outgrown them as she loved having the neighborhood kids come over to play. She often kept popsicles in the freezer for them so when they stopped by, they could have a treat.
Patricia passed away Feb. 28, 2019, at a care facility in Oregon.
Patricia is survived by her four children, Sam (Doris) Thomas of Atlanta, Mark Thomas of Atlanta, Kim Solko (Bob) of Brookings, South Dakota and Tricia Larson (Ryan) of Albany, Oregon; seven grandchildren, Megan Thomas, Paige Thomas, Jake Thomas, Kyle Hanson-Arenas (Ariel), Emilee Hanson, Jordan Hanson and Randy Larson; sister, Gwendolyn White of San Diego, and her nephew, Steven Wedeking II.
She was preceded in death by her parents, Rubin and Jewel White and her husband, Chris Thomas.


Felicia Korman, 25, working at the Moorish Pavilion as grapich designer and with marketing/communication.
Born in the small coastal village Åhus in the south-east of Sweden. Moved to Malmö in 2006 to study a bachelor of Media and Communication and is now writing her master thesis in Culture & Media Production. Enjoys photography, painting, writing music and perfoming as a singer /guitarist.


It's still standing. Somehow. There's not a shard of glass left on the structure and the doors were removed a long time ago. It's no longer a shelter, no longer a square metre of space you could go to talk without distractions and yet it is still functional. Pick up the receiver and after a silent second the repetitive purr of the dial tone still sounds in your ear. Listen carefully though and you'll hear the final breaths of the condemned. The booth knows that it is finished, carrying a sickness that nobody wants to treat. Soon it will be taken away and buried.
It had a good life. We all have memories of important conversations we have had on public payphones, perhaps talking to loved ones from afar whilst the windows of the booth steamed up around us. Now it is obsolete. We have moved on. We communicate from miniature phone booths in our pockets or on other forms of social media, and no longer want to buy cards and queue in the cold before making an important call.
"What would the cinema have been like without me?" it asks. It gave anonymity to the wicked and trapped the innocent. It was the principal character in a film not even ten years ago, but that may as well be ancient history today. It's a washed up old actor now and nobody would give it creedence anymore. Younger, smaller characters have taken over, rapid and mobile and completely disposable. There will be no comeback.
"Look at me" it asks, "take one last long look for soon I will be gone and you won't even remember that I existed. You certainly won't remember what I looked like".
"I will always remember you" I say, "but I don't want to remember you like this".
One of my fondest memories of Paris is from the very first time. I was sitting in a bistrot having a baguette and café, when a little white van drove up. The driver got out his cleaning materials and proceeded to wash down the cabine téléphonique. I had never before (or since, for that matter) seen anything like that.
I miss the old phone booths. I loved the privacy and the feeling of being in your little world on a street corner.
...and, on a different note, I will always remember them from the movie Ascenseur pour l'echafaud for the scene where Jeanne Moreau desparately tries to contact her lover.
Un très beau texte pour une réflexion très juste : pourquoi des téléphones publiques alors que l'individualisme se fait forcené ?
Lorsque j'étais étudiante, j'appellais mes parents depuis une cabine à la poste, en PCV. Parfois une fois par semaine, parfois une fois par mois.
Alors tant pis pour les personnes qui comme moi n'ont pas de téléphone mobile (ni de montre).
Lenox, Cergie, Gina: You all sum up the unique attraction of the phone booth. A phone call was an event, and the booth was the stage. Before the call there was a build up of excitement, stress, fear or dread, with a seemingly endless stretch of time before the person picked up at the other end. You were then cocooned in that little stuffy space for the duration of the call. When the receiver was placed back in position the moment was over and you could go back to the real world. It is no wonder that it was used so often in the cinema. There is no drama in a mobile phone call.
On a related note, I was in England recently and although the famous old red metallic booths have almost disappeared, there are quite a few of a new generation where people can send text messages or e-mails too. In France though, a country that likes to see itself as being at the forefront of telecommunications technology, it seems that they have already signed the death warrant for the phone booth.
What is the situation in your country?


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La Marque celtic bagpipers
Whether celebrating new love at a wedding or commemorating a loved one at a funeral, Celtic Bagpipers can beautifully carry on your culture's musical tradition. Read reviews and book any of the top-rated musicians near you in the La Marque, TX area.
La Marque, TX Celtic Bagpipers
Please note these Celtic Bagpipers will also travel to Hitchcock, Texas City, Dickinson, Santa Fe, Arcadia, Bacliff, Galveston, League City, Kemah, Seabrook, Webster, Alvin, Friendswood, Liverpool, La Porte, Port Bolivar, Crystal Beach, Danbury, Pasadena, Pearland, Manvel, Deer Park, South Houston, Beach City, Cove, Baytown, Rosharon, Arcola, Gilchrist, Angleton
Top Celtic Bagpipers Near La Marque, TX
Are you planning a wedding in La Marque, TX? We can help you find the best vendors and entertainers for your upcoming wedding.
Richard Kean
Bagpipes from Houston, TX (36 miles from La Marque, TX)
My name is Richard Kean, and I am a professional bagpiper living in the south Texas area (close to Houston). I have been playing the bagpipes for 30 years and perform music at a very high standard. In addition to bringing an experienced respect for the Scottish musical tradition, I wear the traditional Scottish attire for events. If possible, I will play requested songs, and I am more than happy to speak with you regarding questions you may have about bagpipes and about playing bagpipes at... (more)
Toddytone Bagpipes
Lyric Todkill is a professional piper and Pipe Band Director at St. Thomas' Episcopal School. Under his instruction the band has won two American Pipe Band Championships, the North American Pipe Band Championship, and the World Pipe Band Championship in Glasgow. Lyric competes with the current World Champion St. Thomas' Alumni Pipe Band who were recently upgraded to grade one, the highest competitive level in pipe bands. Lyric is also a certified adjudicator for the Eastern United States Pipe... (more)
Lary Fowler Aka Leghorn The Piper
Celtic Bagpipes from Brenham, TX (103 miles from La Marque, TX)
Lary is an award winning competition level performance bagpiper.He has performed at most every traditional bagpipe venue but enjoys piping in non-traditional venues such as baseball games and motorcycle rallies. Both public and private schools have asked him to perform at multi-cultural programs, most recently performing in the Rio Grande Valley of Texas. His band experience includes several traditional bagpipe bands as well as guest performances with The Rogues, Scottish Mayhem and... (more)
Jimmy Mitchell
Bagpipes from Forney, TX (249 miles from La Marque, TX)
Jimmy has been playing the bagpipes for over twenty-four years. He has performed at numerous occasions over the years including weddings, funerals, and parties. Since he is a full time bagpiper he can be available on short notice and can be wherever you need him. Jimmy has performed all over the United States and has played in Canada and Scotland. Having learned under some of the finest teachers in the world, Jimmy is a first class player who you can be confident will provide that... (more)
Jared Malone
Bagpipes from Garland, TX (264 miles from La Marque, TX)
Bagpiper for all occasions. I have played many events including weddings and funerals. I have been asked to join a band that is playing in Glasgow, Scotland at the World Pipe Band Championship 2011. I will be in Scotland competing against the best pipers in the world. I am also a former member of The North Texas Caledonian Pipes and Drums and have played at venues such as the State Fair of Texas and the North Texas Irish Festival - just to name a few. Time constraints required me to turn in... (more)
Celtic Bagpipes from Denver, CO (916 miles from La Marque, TX)


Built in 1927 and situated in the heart of one of Santiago's most charming neighborhoods, this city mansion has been renovated and converted into a boutique hotel with excellent personal service. Museums, galleries, trendy shops, antique stores, and innovative restaurants are close by. The hotel offers a restaurant and lounge, swimming pool, and charming interior courtyard.
Location was fantastic. Neighborhood really charming and the room at the top, with the outside patio, was very comfortable. Really like the backyard garden/swimming pool area, although we had little time to enjoy it. Felt like a great place to relax.
We especially enjoyed the backyard garden and pool of the Lastarria Hotel in Santiago, as well as our private decks and the location--near wonderful restaurants.
Lastarria Hotel: very nice; good service; great location.
Lastarria Boutique - excellent accommodations, very helpful staff, very good breakfast, excellent location.


This paper proposes that OPA 90's current limits for ships are realistic, adequate, allow for necessary increases, and incorporate well-tested, proactive spill response mechanisms. The removal of the shipowner's right of limitation would cut across the 'polluter pays' principle, the bedrock of OPA 90, which preserves a balance between shipowner and cargo owner/receiver liability. A shipowner must be able to insure its liabilities otherwise he cannot trade and revising any part of this equation could prejudice the continuance of oil imports to the United States.


The comfortable 2-Room-Apartment is located on the top floor of a Bavarian style home next to the city centre of Garmisch and its pedestrian zone. All shopping facilities and bus stop are nearby, hiking trails, ice skating rink, indoor swimming pool with sauna and fitness centre, tennis courts and cable railways are within walking distance. Even located at a very busy road the apartment is quiet, because the bedroom and the living room are situated at the back of the house.
The comfortably furnished apartment has a nice balcony with views the south and an inspiring mountain panorama. It has a living room with a studio couch, a bedroom with a double bed (180x200), a fully equipped kitchen with dishwasher and breakfast nook and a bathroom with bathtub and toilet.
The apartment is fully equipped for maximum 2 persons and a baby. Colour Cable TV and radio are available. In the basement there is a washing machine for the use of our guests. There is parking in the yard for our guests (in front of the litter container).
The prices are for 4 persons. Every additional person costs € 10,00. End cleaning is included when guests stay a week or longer.
We can provide you with bedlinen, 1 large and 1 small towel (cleaning fee € 7,20 per set), a baby bed (€10,00) and a high chair (€ 5,00). Kitchen towels and cleaning material are in the apartment.


Take on the chilly weather in style with the Women's Jack Murphy Dot Quilted Jacket. A new addition to the collection, this jacket boasts a country inspired diamond quilted outer, as well as contrasting baffle quilting to the outer collar, cuffs and pocket panels to keep you looking on-trend through this season and beyond. Sure to be your perfect partner whether you are heading out on a country pursuit or you are simply popping into town, the Dot Jacket has been crafted from a water resistant outer to keep those unpredictable showers at bay. With pockets to the front and interior, this practical jacket is finished with an adjustable drawcord to the waist so that you can create a feminine, figure flattering silhouette, or a more relaxed feel, ideal for those days when more layers are needed.


(Chicken) Antibodies 101 (Chicken) Antibodies 101 – Aves Labs, Inc.
What is the difference between the "IgY Fraction" and the "Affinity-Purified Antibody Fraction?"
How much IgY is present in chicken eggs?
How does chicken IgY compare methodologically with mammalian IgG?
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Blind, impartial panel tastings (seventeen posted so far – ninety-nine wines tasted in total) of B.C. pinot noirs are currently held approximately every three weeks with wines, thus far, provided mostly by us. Enthusiasm for this project necessarily entails a certain casual disregard for personal finances and you may notice the web site has "no visible means of support". To sample and keep current with over two hundred B.C. pinot noirs by tasting six wines every three weeks makes for a long tasting cycle. If additional wines were available for review by way of trade samples, the tastings would be expanded and/or held more frequently.
Vintage variation, the age of the vineyard, the vagaries of weather at a particular site, storage and transportation, changes in winemaking practice and a range of other factors present in a particular bottle can affect how a wine shows in our panel tastings. We do our utmost to ensure that every BC pinot noir (as well as our "guest pinot") shows its best. Wines for our tastings are stored quietly at cellar temperature (in some cases for years) before being in a tasting. We also do not publish a review of a wine that upon opening, we agree has a serious technical fault.
Library wines , vertical tastings, discontinued/experimental bottlings (e.g. combined block wines that have subsequently been bottled as separate blocks).
Submitted trade samples of B.C. pinot noir, will be panel tasted according to the panel tasting process and the results of the tasting published on the web site and in social media. Wine samples will generally be evaluated in the order in which they are received and submitted trade samples will be given priority in the tasting cycle. The tasting order will however also be in balance with the overall goal of tasting pinot noirs from every B.C. winery and the grouping of tastings by year or theme. We will clearly state within the tasting notes that the wine was received as a sample. Please submit 2 samples of each wine in case of spoilage (this has happened several times at our panel tastings).
There is no gain in being harshly critical, cutting or dismissive in a wine review but be aware that depending on what is found, the praise may be faint and that any criticism proceeds from a spirit of providing an objective, honest and constructive reflection of the quality and value of BC pinot noir both for the industry and for the world at large.
Samples of non-pinot noir wines from B.C. are also accepted but will be individually tasted (i.e. not by the full panel) and if a review is published it will be in a separate non-pinot blog posting on the web site. If there are certain aspects of any wine that you are looking for feedback on, please indicate that when sending the wine. Note that if we do not care for your wine we may not post a review at all.
We are not accepting international wines for samples at this time (there is a separate web -based project coming from us for international wines). We do accept submissions related to wine accessories, food and wine related travel. We do however, make no guarantees that all products will be reviewed.
Winemaker notes or details about the wine (electronic version preferred) including biodynamic, organic, dry farmed, vinification techniques, technical information etc.
Extra information you submit will be included as notes on the wine in our reviews.
Ste. 1002 – 5805 Balsam St.


The eye check mirror is a great tool to watch your students eyes as they navigate the roadways. It is 3 inches in diameter with a small suction cup to adhere to the glass. It is sold as a set with a small rear view mirror. The rear view mirror is 5.5" x 2" and also has a small suction cup to adhere to the glass.
Use the drop down menu to order, then add to cart.


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Miami-Dade delegation already discussing immigration legislation
Miami-Dade's legislative delegation yesterday discussed immigration reform — another signal that the controversial issue will be important in Florida's 2012 legislative session.
The Miami Herald reports today:
The Miami-Dade delegation met for the second time this month on Wednesday — not [to] take positions on controversial issues, but to listen to nonprofits asking for lawmakers' help in Tallahassee.
Yet the first group to speak asked about immigration, perhaps the most contentious issue in this year's session. And that prompted Democratic state Rep. Luis Garcia to "demand" that his colleagues vote to stand against any anti-immigrant proposals that could crop up in session. Gov. Rick Scott has said the issue is still one of his legislative priorities.
Scott, who agrees that state law enforcement must be allowed to ask suspects about their immigration status, signed an executive order requiring that all state agencies — and all companies that enter into contracts with state agencies — use E-Verify to check the employment elligibility of their workers.
The Herald adds:
State Rep. Carlos Lopez-Cantera, a Republican and the delegation's chairman, said legislators would meet in Miami-Dade later this year to vote on their priorities. He pointed out that most, if not all, of Miami-Dade lawmakers had either taken a public position or voted against immigration proposals in the past — but warned that the issue would likely not, in his view, qualify as a delegation priority.
In its spring session, the Florida Legislature failed to pass immigration enforcement bills that would have allowed local law enforcement among other things to to ask suspects about their immigration status.
Two failed immigration enforcement bills — state Rep. William Snyder's H.B. 7089 and Sen. Anitere Flores' S.B. 2040 — included provisions that would make programs such as Secure Communities a permanent part of law enforcement in Florida.
Problems with Secure Communities, a federal immigration enforcement program, has already prompted elected officials from other states to opt out of the program. The program counts the Florida Department of Law Enforcement and all of Florida's counties (.pdf) as participants.
Secure Communities allows local law enforcement agencies to check the fingerprints of people they detain and match them up with federal immigration and criminal databases, with the stated goal of deporting criminals. Immigration and Customs Enforcement, ICE, announced last week that the federal government will no longer rely on agreements with states to operate the controversial program.
A report released in March indicated that according to ICE data, five Florida jurisdictions where Secure Communities is being implemented are among the top 20 jurisdictions with the highest numbers of deportations of non-criminals. In Miami-Dade and Hillsborough counties, 66 percent of Secure Communities deportations were of non-criminals.
Feds release state health exchanges guidelines as Florida continues to stall implementation
Occupy Miami picks up union support
Occupy Wall Street supporters arrested in Tampa and Orlando
Last week, protesters with Occupy Tampa and Occupy Orlando were arrested. The groups were both rallying in solidarity with the Occupy Wall Street movement that has spread all over the country.
Florida seafood industry poses questions for Feinberg: News. Politics. Media
The Gulf Coast Claims Facility has been paying claims to tourism businesses affected by the BP oil spill, but a fishing industry representative told the Florida Senate Agriculture Committee that another group of businesses — seafood wholesalers — may not be faring as well.
CAIR: 'Anti-Shariah' law shows Sen. Hays 'disconnected' from 'real problems'
Responding to news that state Sen. Alan Hays, R-Umatilla, has filed what one right-wing organization is dubbing an anti-Shariah law, Nezar Hamze, executive director of the South Florida chapter of the Council on American-Islamic Relations, tells The Florida Independent that Hays' bill could impact believers in all faiths and that its filing shows Hays is disconnected from his constituents and Florida's real problems.
Gulf Coast residents in dire financial straits waiting for BP claims
Christine Watson, of Destin, Fla., has to come up with $1,900 this week or she'll be evicted from…
Residents of historically black Miami neighborhood demand jobs
The Overtown Alliance and dozens of residents met face to face with representatives of the University of Miami…
Rep. Alan Grayson to Obama: Fire McChrystal
Rep. Alan Grayson, D-Orlando, via The Huffington Post: Let's face it. McChrystal has destroyed his working relationship with…