L'ÉCOLE Van Cleef & Arpels, first of its kind in the world, made its début in Hong Kong on October 16th, 2014; the nomadic school of jewelry & watchmaking took up residence at PMQ for the next 17days. L'ÉCOLE Van Cleef & Arpels offered a range of courses and conversations designed to illuminate and to inspire to lovers of beauty, enlightened connoisseurs and professionals. The Maîson requested the designer to conceive a total branding for L'ÉCOLE Van Cleef & Arpels, which shall capture the essence of L'ÉCOLE Van Cleef & Arpels in Paris with the embodiment of local éclat wherever the school is visiting. The selection of materials, color tones and details was carefully fine-tuned to set L'ÉCOLE Van Cleef & Arpels apart from the boutiques without losing its heritage.


text:
Non Toxic Living with Sisel International
Last Updated on March 1, 2018 by Katie Sisel Distributor
***DISCONTINUED****
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This blend of powerful ingredients was created to deliver incredible thermogenic support. Thermogenesis, or the creation of heat, is a process concurrent with the burning of calories, and as anyone who has struggled with diet and exercise knows, burning calories is not easy!
With Accelerator it can be!
Not only do the ingredients in Accelerator work synergistically with each other, but as a formula Accelerator works in concert with our other thermogenic product, ThermoCharge. Because these two formulas are so different, their ingredients function through entirely different mechanisms of action to power up your calorie and fat burning efforts.
Accelerator can be the perfect supplement to initially jumpstart your weight loss efforts, and when possible should be among the first of the five products included in Sisel's Slim & Slender Rapid Weight Loss System that you use.
Throughout the world, only water is consumed in higher quantities than tea, and few plants have been studied more for their potential health benefits. Green tea has active antioxidant properties and has been shown to have thermogenic effects in humans. In addition, it may suppress fat accumulation. In fact, in a 90-day study, human participants consuming 150 mg of green tea extract lost 14 kg compared to a loss of only 5 kg in the control group.
This naturally occurring alkaloid, found in tea, cacao, kola trees and dozens of other plants, is commonly consumed around the world. As a central nervous system stimulant, caffeine my increase resting energy expenditure and promote thermogenisis. In humans, 200 mg of caffeine has been shown to increase metabolic rate by 7% in just three hours.
Derived from red chili peppers, capsicum is thought to work on the sympathetic nervous system to increase thermogenesis. When combined with green tea, this effect may be sustained for a longer period of time. Capsicum has also been proven, in a laboratory environment, to promote lipid mobilization from adipose tissue.
As a bioavailability enhancer, black pepper may assist in the absorption of other ingredients. However, by itself, it has been shown to increase energy expenditure by promoting ATP activity in the mitochondria of cells in the body. It may also support increased pancreatic function in order to enhance digestive capacity. It is also a powerful antioxidant in its own right.
This water-soluble vitamin may be a metabolically active substance because it has been shown to be scarcer in obese individuals. It is also utilized in the body for energy metabolism and electron transport chain activities.

redpajama_set_name: RedPajamaCommonCrawl


text:
'Bachelor in Paradise': Five Thoughts After Week 3
Home » 'Bachelor in Paradise': Five Thoughts After Week 3
Alyssa Barbieri
When Bachelor in Paradise closes the beach after this sixth season, there's a spinoff that's just begging to be created: Stagecoach.
Every time a woman walks into Paradise, Blake seems to not only like her, but know her. And not just from anywhere. From Stagecoach.
The California-centered country music festival has been the stomping grounds of Blake developing into one of the biggest turds in Bachelor Nation, where he's either been kissing, having sex or talking, and hoping it leads to the former, with pretty much every girl that's walked onto this show.
This is either the best free advertising or the most brilliant PR stunt The Bachelor franchise has ever developed.
Here are five thoughts after the third week in Paradise.
1. Does Blake know every woman that comes to Paradise?
I'm so beyond over the Blake saga that I dread each week because I know that Blake will probably meet a new girl he met at Stagecoach. Seriously. It's like the producers decided to bring in everyone at Stagecoach — and Blake just happened to hook up with a lot of them. If he wasn't the most vile thing I've ever seen in Paradise…
2. Dylan and Hannah are the cutest thing in Paradise
It's amazing how you don't realize you have a good thing until it's gone. Luckily for Hannah, Dylan was so amazing that she didn't have to lose him to realize she'd made a mistake teetering between him and Blake. As far as opposites go, Dylan is the antithesis of Blake in all the right ways. He's been patient, he's been honest, he's been open, he's been vulnerable and he actually wants a relationship instead of a Stagecoach Jr. hookup. I'm just glad Hannah realized it. Now, we're treated with the two of them acting like adorable puppies, and I'm emotionally compromised.
3. There's a fine line between reality TV drama and violence
Bachelor in Paradise had done a lot to hype up the fight between Jordan and Christian, which escalated on Monday when the two went at each other's throats. While it was good TV, it was evident immediately that it was more heated than Paradise producers might've realized. Christian had to be held back by multiple security guys as he tried to go after Jordan, who knocked over a pinata when Christian was trying to woo Nicole. But Jordan and Christian's history goes back to their Bachelorette days, where the two went at it at the Men Tell All. These producers definitely were in way over their heads on this.
4. Demi and Kristian open the door in Bachelor franchise as first same-sex couple
The Bachelor franchise hasn't exactly been diverse in ways that it should. It took years for them to have their first black Bachelorette, Rachel Lindsay, and there hasn't been another since. Mike appears to be the most likely next option. As for same-sex couples, it never seemed like something the show would explore. Perhaps Paradise was the perfect place for the franchise to give it a go. Demi revealed last week that she had been dating a woman back home before deciding to come to Paradise, and it was a great conversation between her and kinda-boyfriend Derek. This week, Kristian came to Paradise to show Demi that she wanted to pursue their relationship. It was like the heavens shone down on The Bachelor franchise, as it offered representation in a way it hadn't before.
5. Derek just auditioned for The Bachelor and may or may not realize it
While Derek might've gotten his heart stomped on — for the millionth time — in The Bachelor franchise, it might not have been without a benefit. Usually, we don't get The Bachelor announcement until after Paradise, in case of any interesting developments out of Paradise prompt a new direction. Like Nick Viall a couple years ago. While Tyler C. doesn't appear to be interested in being The Bachelor — dating Gigi Hadid — that leaves Peter and Mike, the latter of which is also on Paradise. But there might be a dark horse in the competition in Derek, who showed that he was a forgiving and understanding man when Demi came out being bisexual and breaking things off with him to pursue a relationship with her girlfriend. Derek's understanding — and the maturity with which he handled it — certainly bode well for his chances, even if they are slim.
Bachelor in Paradise airs Mondays and Tuesdays at 8/7c on ABC.
Tags ABC Bachelor in Paradise Reality TV The Bachelor The Bachelorette
Alyssa is the Managing Editor for Bears Wire. You can reach her at alyssaabarbieri@gmail.com or follow her on Twitter @AlyssaBarbieri
'Younger' 6x10 Review: "It's All About Money, Honey"
'Villains' Trailer Premiere Delivers on the Crazy and Unexpected
'The Rookie' 3×03 Review: "La Fiera"
'The Bachelor' 25×02 Roundtable: "The Queen" Must Go
'The Bachelorette' Star Zac Clark Talks Staying Sober Through Filming
'The Bachelor' 25×01 Roundtable: And We're Back…


As a publisher producing online-only journals, securing the long-term archiving of all published articles is a central issue for us at Shared Science Publishers. In fact, scholarly knowledge represents a precious good, the preservation, protection and propagation of which needs to be ensured in the future, including beyond a publisher's lifetime.
Specifically for an electronic journal, it is vital that a version of record (VoR) is deposited at an official and independent repository. A VoR is as defined by the National Information Standards Organization, in partnership with the Association of Learned and Professional Society Publishers, "a fixed version of a journal article that has been made available by any organization that acts as a publisher by formally and exclusively declaring the article 'published'. This includes any 'early release' article that is formally identified as being published even before the compilation of a volume issue and assignment of associated metadata, as long as it is citable via some permanent identifier(s). This does not include any 'early release' article that has not yet been 'fixed' by processes that are still to be applied, such as copy-editing, proof corrections, layout, and typesetting." Depositing such a VoR guarantees that any reader will have perpetual access to the unaltered content of the original publication.
The VoRs of all articles published by Shared Science Publishers journals are protected by our partnership with and deposition of content at the Austrian National Library and Pubmed Central.


Beechcraft Corporation, a subsidiary of Textron Aviation Inc., a Textron Inc. company, has launched a new demonstrator dedicated to showcasing the Intelligence, Surveillance and Reconnaissance (ISR) capabilities of its Baron G58 piston-engine aircraft.
The demo aircraft is scheduled to begin its tour in North America this month with its first stop at the Airborne Law Enforcement Association Expo July 16-19 in Phoenix, Ariz. The aircraft will also attend the Latin American Business Aviation Conference & Exhibition (LABACE) Aug. 12-14 in Brazil to begin a tour of South America, before completing the year with a tour throughout the UK and Europe.
Beechcraft has long served the ISR markets with its range of King Air turboprops and first introduced a version of its twin-engine piston Baron G58 aircraft with ISR capabilities in 2012 at the Farnborough International Airshow. The company delivered the first Baron G58 with an ISR package in 2013 to the Fuerzas Unidas de Rápida Acción (FURA), an agency within the Puerto Rico Police Department that uses the aircraft for law enforcement surveillance missions within the region.
The Baron G58 ISR package includes a FLIR 230-HD electro-optical/infrared (EO/IR) camera system, an operator's console that houses the mapping/mission management computer, a recorder and a multi-band communications radio system and data link for special mission operators. The 230-HD EO/IR camera has the ability to virtually see both day and night, over land and water, in both good and poor weather. The mission mapping system provides the camera operator with a visual representation of the target location. The multi-band communications radio gives the flight crew and the sensor operator the ability to communicate directly with the military, police vehicles, coast guard and other maritime vessels and the recorder provides court admissible evidence for prosecution.
All of the surveillance mission equipment for the Beechcraft Baron G58 ISR is commercially available. While the physical integration of the mission sensors to the airframe is highly specialized, the mission package integration is done without customized software, making it easy to troubleshoot and maintain in the field.


This collection of High Potency Omega-3 products are the most potent and pure fish oils on the market today. With any of these it is very easy to achieve your daily goals of EPA and DHA-usually about 3000mg for most people based on our typical diets. All of them are among the lowest cost supplements per mg of EPA and DHA while also being some of the cleanest oils from the most sustainable fisheries on the planet!


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Home News 30 Years Ago: Metallica's Old Bassist Cliff Burton Dies In Tragic Bus Accident
30 Years Ago: Metallica's Old Bassist Cliff Burton Dies In Tragic Bus Accident
Metallica have shared an emotional video from the family of former bassist Cliff Burton on the 30th anniversary of his death, as well as launching a Facebook and Instagram page in his memory.
Burton was killed in a bus accident when the band were travelling through Sweden on tour on 27 September 1986. To mark three decades since his tragic passing, the band have shared a video of an appeal from his father Ray.
"I just want to talk to you very briefly about the anniversary coming up of the accident that took Cliff away from us," he said. "It'll be a little history. Some of it is from the beginning when Cliff was born in 1962 and I will try to convey some things that I found extremely loving of the young man. He was quite a young man to say the least.
"From a very, very young age he showed his mother and me examples that he was pretty extraordinary – even as a young child in school."
To remember the late bassist, fans are invited to share their tributes on a new Facebook page set up in his memory.
"We would like your comments, if you have photographs or want to play music," said Ray. "We just want to wish you the best and have a happy new year."
via: nme.com
Oct 9, 2016 Metallica Online
Which Metallica Album Are You?7 Less Known Facts About Metallica You Probably Didn't Know
Watch, Metallica's Amazing Live Performance of "FUEL" From Their Latest Show ( 05.19.2017)
Metallica Bridge Their Past and Future at Electrifying Club Gig Performance
October 9, 2016	News, Old MembersCliff Burton death, Cliff Burton Family, Cliff Burton Metallica
Watch, ROBERT TRUJILLO Pays Tribute To CHRIS CORNELL During Foxborough, Massachusetts Concert
KIRK HAMMETT On TRUMP Presidency: "I Don't Know What The F**k's Gonna Happen"
This Man Plays Metallica's "Kill Em' All" Album In Major Key, And We Can't Get Over How Perfect It Sounds!
Subscribe to our Metallica mail list and get important news and updates before eveyone.
Watch: Kirk Hammett Credits Soundgarden As Inspiration for "Enter Sandman" Riff
Watch Video Of 'For Whom The Bell Tolls' Performance From ROCK ON THE RANGE
Metallica Are Copping It For Their Cover Of Prince's 'When Doves Cry'
TikTok Star Shreds Metallica Songs After Being Mocked For Wearing One Of Their Shirts
Hear The HU Deliver A Mongolian Metal Cover Of Metallica's 'Sad But True'
Watch Metallica, Members Of Soundgarden And More Cover Alice In Chains During Tribute Concert


Learning to Eat Allergy-Free: Lots of Winners!
First, I must thank all of you for supporting this blog, and for helping to vote me into the Top 25 Food Allergy Blogs at Circle of Moms. I consider myself very lucky to be within the top 10, and very grateful for everyone's support. I am in exceedingly good company with some truly awesome bloggers. Be sure to check out the whole list!
Oh! Lucky winners, you are going to love this prize!! I have e-mailed each of you. Please send me with your mailing address no later than Friday, November 25th.
And (yup, I'm not done giving way stuff) – if anyone is interested, I still have one coupon for a free Goodbelly product to be given away. The first person to e-mail me here with their address gets it.


text:
Q: android: how does facebook messenger interact with my activitity lifecycle? I'm trying to learn how to develop Android and I'm currently following this tutorial on the activities lifecycle: https://developer.android.com/training/basics/activity-lifecycle/pausing.html
I created a very simple code to check the triggering of the onPause() and onResume() functions, e.g. for onPause() I just wrote the following in one of my activities (and I did the same kind of stuff for the onResume() function):
@Override
protected void onPause() {
    super.onPause();
    Toast.makeText(this, "ON PAUSE", Toast.LENGTH_LONG).show();
    Log.i("STATUS_ON_PAUSE", "ON PAUSE");
}

When I start/stop the activity or when I switch from one activity to another I do see the corresponding Toasts and logs. But I don't see anything when I overlay a facebook-messenger conversation over my activity. No Toast when opening the conversation (I would have expected to see "ON PAUSE"), no Toast when giving the focus back to my application.
I tried to inspect the log-files on a broader level (my device) but I'm still not good enough to really understand what goes on there (way too much information).
So, my question is: how come my activity is not paused/resumed when I give/remove focus to a chat-head? Thanks!

A: The simplest answer would be:
Messenger is adding a view to a Window instead of starting a new activity.
This is why Messenger needs SYSTEM_ALERT_WINDOW permission and you can see that when you see it's details:
"draw over other apps" permission.
You can see THIS ARTICLE

*

*You won't get onPause or onResume when someone is drawing over your
window.

*You will always get onPause and onStop when your activity is covered
with other activity.

*You will always get onPause and no onStop when your activity is
covered with a dialog styled activity (so that your activity is
still visible in the background).

*You will not get onPause or onStop when your activity is covered by a DialogFragment - this may look exactly the same as a dialog styled activity but there is a difference in lifecycle and implementation.

redpajama_set_name: RedPajamaStackExchange


text:
Q: How can I make discord not show what game I'm playing? Discord will show what game you're playing, but I don't want it to do that.
Is it possible to make discord not show the game currently being played?

A: From this post:

User settings > Games > untick Display Currently running game as a status message

A: The web version will show the game you are in.  The Web version does not have the Games tab that allows you to change this options.


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text:
Gemeinschaft Home
Racial Equality Statement
Employees & Staff
Residential >
Day Reporting Center (DRC)
Self-Pay Program
A Taste of Home: Soul Food for A Cause
Brenda Leigh provides Gemeinschaft Home residents with family-style meals, made daily from scratch with fresh, high-quality ingredients.
Food Services Manager, Brenda Leigh
The sweet and savory aromas of home cooking quickly envelope the senses of anyone who walks through the front doors of Gemeinschaft Home in the early afternoon. Dinner is served daily at 4PM, but the irresistible scents that reach into every corner of the house often lure residents to the dining area earlier. Even some of the administrative staff members, whose offices are located on the first floor of the house, admit to venturing back into the kitchen periodically in search of one fragrance or another swirling in the air.
Food manager, Brenda Leigh, established the practice of preparing fresh, home-cooked meals for residents, when she joined the Gemeinschaft Home staff just over three years ago. At first, Leigh took charge of the kitchen on a temporary basis, and she characterized her role at that time as just "helping out" the organization while they hired someone to fill the position. However, shortly thereafter, she was offered the job permanently, and the Gemeinschaft Home community has enjoyed the benefits of her culinary skill ever since.
Leigh, who is known affectionately among the residents as "Ms. B."—not just because her first name begins with the letter B, but she is often seen "as busy as a bee around the kitchen," according to one former resident—is not professionally trained in the culinary arts, but she brings a wealth of experience to her work at Gemeinschaft Home. Much of her skill comes from her past work in the food industry, the lessons she learned as a child in her grandmother's kitchen, and the years she spent raising children and preparing family meals.
Because the residents' work schedules differ greatly, and many are at their job locations during breakfast and lunchtime, Ms. B. prepares brown-bag meals for residents each day as well, and she maintains a well-stocked cupboard for residents (who cook for the house on the weekends) to prepare meals.
Ms. B.'s commitment to the wellbeing of the residents is most evident in her philosophy of soul food. To her, having access to good food is an integral part of the life recovery program at Gemeinschaft Home. The majority of residents arrive with at least one year of prison food behind them, and she argues that a little taste of home can help to ease some of the anxiety and stress that many individuals experience upon leaving prison.
Prior to her arrival three years ago, residents consumed meals prepared mostly from pre-packaged, processed, and frozen ingredients, but Ms. B. saw an opportunity to give residents tastier, more nutritious foods, including many of her own recipes as well as those shared by residents—"They teach me!" she proclaims proudly. Her reputation is well known among some prospective residents, who have heard about her home-cooked meals from their friends or acquaintances who have lived at Gemeinschaft Home.
Ms. B. stresses that good food is healing for the soul, and she points out that no matter what issue an individual resident may face, he knows that he will eat well today and every day he lives at Gemeinschaft Home. In addition to the daily meals, Ms. B. also prepares special dinners on major holidays such as Thanksgiving, Christmas, New Years Day, and Easter.
The gratitude from residents is readily apparent, according to Ms. B., who claims she often hears comments such as "This tastes just like my mom used to make!" or "This is the same way my grandmother made it!"
While she has made more money working in other positions, Ms. B. says that no other job has ever been as rewarding. The residents make her job worthwhile, and she is happy to contribute to their success in the program.
While she knows that residents have a long journey ahead of them, with a number of issues confronting them, she asks herself, "If I were in their shoes, how would I want to be treated?" The answer can be found in the main ingredient of her cooking—love—and she always dishes out an extra helping.
1423 Mt. Clinton Pike Harrisonburg, VA 22802
Phone: 540-434-1690
Fax: 540-432-3086
© 2014-2020 Gemeinschaft Home, 501(c)(3) nonprofit
Website by J.L. Jacovitch & Associates


text:
Lubrizol buys Dow's TPU business
Ohio-based Lubrizol Corporation has announced that it has purchased the thermoplastic polyurethane (TPU) business f...
January 5, 2009	Canadian Plastics
Ohio-based Lubrizol Corporation has announced that it has purchased the thermoplastic polyurethane (TPU) business from Midland, Mich.-based Dow Chemical Company.
The acquisition will help Lubrizol expand it's Estane brand engineered polymers business. Dow's TPU business had 2007 revenues of approximately US$85 million. The deal closed on December 31, 2008, and the terms of the deal were not disclosed.
Dow's TPU business is located in La Porte, Tex., and the product line includes everything from elastomeric to rigid TPU properties.
"We are excited to be adding Dow's TPU capabilities and products to our existing Estane Engineered Polymers business," said Mike Vaughn, Lubrizol vice president, Estane Engineered Polymers. "Given Dow's strong TPU brand recognition in the U.S., this addition further supports our strategy of growth through geographic expansion as it will allow us to introduce new products and technology to international markets where Dow's TPU business has had limited penetration."
The agreement includes all commercial, production and research and development assets of the Dow TPU business. Lubrizol will assume approximately 40 full-time employees, and Dow's brand names will be retained and "unified" under the Estane Engineered Polymers business.
Schulman buys Perrite Group to boost its engineered plastics business in Asia
Lubrizol to shut Niagara Falls site in 2009
Xaloy buys Dynisco extrusion business (March 28, 2006)
Tough road ahead for Canadian manufacturers: CME survey
CPIA announces energy conservation initiative


For sale: building for a hotel, constructions and land, turnkey. District Tangalle, Rekawa, Deep South of Sri Lanka.
Direct deed, without intermediaries, with the hotel owner, a great friend of Lankarus.
The property is located in Rekawa, one of the most prestigious resorts in Sri Lanka, about 210 km. away from Colombo.
The main building is located just 30 meters from the Rekawa beach with golden sand, and is undoubtedly one of the most beautiful beaches in Sri Lanka, being a mass tourist infrastructure untouched place. The property is framed by a beautiful lagoon.
This exclusive hotel was started to built in 1998 by Russian author. A unique complex consists of 2 blocks — central and beach villas.
In the main unit on the ground floor- provides space for 4 spacious rooms, each with its own bathroom / shower. Two rooms of 4 turned towards the ocean, the other two rooms have spectacular views over the protected lagoon. The first floor is constructed equally to the ground floor. The second floor construction provides a flexible completion of the project — from 2 to 4 rooms (possible according to plan of the ground and 1-st floors). Additional heightened playground on the 2nd floor can be multi-purpose. For example, the completion of the largest and most spacious room or terrace for relaxing or high-altitude restaurant. Completion is possible within a 5-storey building, as construction of the structure has a solid foundation platform from which to make sufficiently large load without building offset threats, deformation or cracking of the walls.
The main building is equipped with auxiliary multi-purpose rooms.
The hotel offers well maintained pool in the middle of a beautiful green garden with exotic flora, which blooms all year round. The swimming pool with breathtaking views of the surrounding area and the Rekawa beach.
Each room can be equipped with a king size bed and a bathroom with a shower with hot and cold water, and be able to offer its own private veranda with views of the ocean or lagoon.
The owner has a full package of permits for construction 6 additional buildings within the land — the beach villas. One of them on its own architectural plan is almost completed on the ground floor and needed decorating and furniture on the 1st floor of the beach villa.
Information on request to Lankarus.
Full support of the deed from the negotiation to the delivery of a package of property ownership documents.
Division of the land property into 3 parts possible. Acquisition of plot with buildings is possible as a whole property, as well as its parts.
Если вас заинтересовал "Hotel For Sale in Tangalle Area", оставьте заявку в произвольной форме. Мы свяжемся с вами в ближайшее время.


Sticky is coming for the project baby.
(AllHipHop News) Sticky Fingaz is coming in hot for Kodak Black.
After hearing "Bust Down," it's clear he's upped the bar.
The rap legend takes his time to address the false accusations from Kodak claiming Fingaz punched him on the tour bus.
In fact, the Onyx rapper claims the story is completely fabricated.
Sticky Fingaz shared the track where he raps, "Picture that, that f##kboy Kodak Black/Punched Sticky Fingaz in the face, well where the f##k was I at?"
Honestly, Sticky did his thing. Can't wait to hear what Kodak has to say!


text:
package edu.duke.oit.solr.test

import org.specs2.mutable._
import org.apache.solr.common.SolrInputDocument
import org.apache.solr.client.solrj.SolrQuery
import org.apache.solr.common.SolrDocumentList

import edu.duke.oit.vw.solr._
import edu.duke.oit.vw.models._
import edu.duke.oit.test.helpers.TestServers

// use scala collections with java iterators
import scala.collection.JavaConversions._

class SolrModelSpec extends Specification with Tags {
  val widgetSolr = TestServers.widgetSolr
  val vivoSolr   = TestServers.vivoSolr

  "A Solr Model" should {

    object TestModel extends SolrModel

    "retrieve a document by id from the supplied solr server" in {
      val doc1 = new SolrInputDocument()
      doc1.addField("id","http://faculty.duke.edu/test/1")
      doc1.addField("json","a string for testing")
      doc1.addField("group","DUKEU")
      widgetSolr.add(doc1)
      widgetSolr.commit()
      val doc2 = new SolrInputDocument
      doc2.addField("id","http://faculty.duke.edu/test/2")
      doc2.addField("json", "this should be found ing1")
      doc2.addField("group","DUKEU")
      widgetSolr.add(doc2)
      widgetSolr.commit()
      TestModel.getDocumentById("http://faculty.duke.edu/test/2",widgetSolr).get("json").toString must_== "this should be found ing1"
    }

    "retrieve a document with a search string from the supplied server" in {
      val result = TestModel.search("southern asia",vivoSolr)
      println(result.numFound)
      println(result.groups)
      println(result.items)
      println(result.items.map(_.group))
      println(result.toJson)
      failure
    }.pendingUntilFixed("Failing currently")
  }

  "The Person Object" should {

    val testPersonJson = """
        {
          "uri": "http://vivo.duke.edu/person1",
          "label" : "Smith J",
          "vivoType" : "http://xmlns.com/foaf/0.1/Person",
          "title" : "Professor of Testology",
          "publications": [
            {
              "uri": "http://vivo.duke.edu/test1",
              "vivoType": "http://purl.org/ontology/bibo/Article",
              "label": "Programming Tips",
              "attributes": {
                "issue": "13",
                "year": "2005",
                "other_uri": "http://vivo.duke.edu/test2323423"
                "authorList": "Lawrence GL, Smith J"
              }
            }
          ]
        }
    """

    // "
    "find an indexed person by uri and return some person instance" in {

      val doc1 = new SolrInputDocument()
      doc1.addField("id","http://vivo.duke.edu/person1")
      doc1.addField("json",testPersonJson)
      doc1.addField("group","DUKEU")
      widgetSolr.add(doc1)
      widgetSolr.commit(true,true)

      val po = Person.find("http://vivo.duke.edu/person1",widgetSolr)
      val p = po.get
      p.uri  must_== "http://vivo.duke.edu/person1"
      p.label must_== "Smith J"
      p.publications(0).label must_== "Programming Tips"
    }

    "not find a non-indexed person and return None" in {
      Person.find("no_chance_this_has_been_indexed",widgetSolr) must_== None
    }

    "know all of its URIs" in {
      val person = PersonExtraction(testPersonJson)
      person.uris must_== List("http://vivo.duke.edu/person1",
                               "http://vivo.duke.edu/test1",
                               "http://vivo.duke.edu/test2323423")
    }

    "contain the uri that matches a value in the extra items that starts with \"http\"" in {
      val person = PersonExtraction(testPersonJson)
      person.uris.contains("http://vivo.duke.edu/test2323423") must_== true
    }
  }
}

class SolrExtractionSpec extends Specification {

  "Extract person name" in {
    val person = PersonExtraction(testPersonJson)
    person.label must_== "Smith J"
  }

  "Extract publications" in {
    val person = PersonExtraction(testPersonJson)
    person.publications mustEqual List(Publication( "http://vivo.duke.edu/test1",
                                                   "http://purl.org/ontology/bibo/Article",
                                                   "Programming Tips",
                                                   Some(Map("issue" -> "13","year" -> "2005"))) )
  }

  "Extract value from publications " in {
    val person = PersonExtraction(testPersonJson)
    val issue = person.publications.head \ "issue"
    issue mustEqual "13"
  }

  val testPersonJson = """
    {
      "uri": "http://vivo.duke.edu/person1",
      "label" : "Smith J",
      "vivoType" : "http://xmlns.com/foaf/0.1/Person",
      "title" : "Professor of Testology",
      "publications": [
        {
          "uri": "http://vivo.duke.edu/test1",
          "vivoType": "http://purl.org/ontology/bibo/Article",
          "label": "Programming Tips",
          "attributes": {
            "issue": "13",
            "year": "2005"
          }
        }
      ]
    }
  """
}

// "
class SolrJsonProducingSpec extends Specification {
  import edu.duke.oit.vw.solr._
  import edu.duke.oit.vw.utils.Json

  "Produce json for attributes" in {
    val ei = new VivoAttributes("uri", "type", "label", Option(Map("a" -> "b", "c" -> "d")))
    ei.toJson must_== """{"attributes":{"a":"b","c":"d"}}"""
  }

  "Produce json from the Json object" in {
    val ei = new VivoAttributes("uri", "type", "label", Option(Map("a" -> "b", "c" -> "d")))
    Json.toJson(ei) must_== """{"attributes":{"a":"b","c":"d"}}"""
  }

  "Produce publication json" in {
    val pub = Publication("http://vivo.duke.edu/test1",
                          "http://purl.org/ontology/bibo/Article",
                          "Programming Tips",
                          Option(Map("issue" -> "13","year"->"2005")))
    pub.toJson must_== """{"uri":"http://vivo.duke.edu/test1","vivoType":"http://purl.org/ontology/bibo/Article","label":"Programming Tips","attributes":{"issue":"13","year":"2005"}}"""
  }
}

redpajama_set_name: RedPajamaGithub


Andy Sullivan has his sights on a South African hat-trick as he launches his challenge for the Africa Open title in East London.
The fast-rising Nuneaton pro has captured two European Tour titles in the space of eight weeks and joked: "I think if I did I'd become one of the most hated people down here, so I'm not sure if I want to win this week. But obviously it would be great to get a victory down here as well.
"It's quite hard because we go from week to week so it still has not sunk in yet. I am on the crest of a wave and just want to keep riding it and hopefully keep going this week. I'm looking forward to the task at hand, I think the wind is going to keep the scoring pretty fair and it should be fun.
Four of the players who finished runner-up to Sullivan in last week's Joburg Open last week – David Howell, Jaco van Zyl, Wallie Coetsee and Kevin Phelan – are in the field at East London, along with Oliver Fisher, who lost out in a play-off to Aiken 12 months ago.
Home players have won the Africa Open every year since it began in 2010, with former Masters champion Charl Schwartzel taking the inaugural event followed by fellow major winner Louis Oosthuizen (twice), Darren Fichardt and Thomas Aiken.
Aiken's win 12 months ago helped him top the Sunshine Tour Order of Merit and secure a place in the field for the WGC-Cadillac Championship, which is also being staged this week at Doral.
That leaves the likes of Fichardt and George Coetzee to lead the home challenge, with Coetzee keen to keep the trophy in South African hands.
Coetzee, who finished 24th in the defence of his Joburg Open, said: "The Africa Open always attracts a strong contingent of local and international players and t The crowds are always fantastic, because they are lively but very neutral. They just want to watch some great golf and they cheer for everyone.


text:
Belén är en ort i Mexiko.   Den ligger i kommunen Apaseo el Alto och delstaten Guanajuato, i den centrala delen av landet,  km nordväst om huvudstaden Mexico City. Belén ligger  meter över havet och antalet invånare är .

Terrängen runt Belén är platt västerut, men österut är den kuperad. Den högsta punkten i närheten är Cerro Godoy,  meter över havet,  km sydost om Belén. Runt Belén är det ganska tätbefolkat, med  invånare per kvadratkilometer. Närmaste större samhälle är Apaseo el Alto,  km nordväst om Belén. Omgivningarna runt Belén är en mosaik av jordbruksmark och naturlig växtlighet.

Ett tropiskt höglandsklimat råder i trakten. Årsmedeltemperaturen i trakten är  °C. Den varmaste månaden är april, då medeltemperaturen är  °C, och den kallaste är december, med  °C. Genomsnittlig årsnederbörd är  millimeter. Den regnigaste månaden är juli, med i genomsnitt  mm nederbörd, och den torraste är januari, med  mm nederbörd.

Kommentarer

Referenser

Orter i Guanajuato

redpajama_set_name: RedPajamaWikipedia


From August 17-21, 2009, the Udall Foundation will host a Parks in Focus trip in Maine with a group of sixth and seventh graders from the Boys & Girls Club of Manchester (New Hampshire). The trip will take them to Acadia National Park. This is the second year that the Udall Foundation has hosted a Parks in Focus trip in Maine.
The Udall Foundation trip leader is Robert Filbin, a 2004 Udall Scholar. Intern leader Jessie Comba, a 2008 Udall Scholar, will be joining the trip. The participants also will be accompanied by representatives from the Boys & Girls Clubs of Manchester.


The Versys-X 300 is the new first rung on the ladder of the Versys Range. With a 300cc parallel twin engine tuned for low and mid-range power, the styling and ride is very much "trail" inspired.
The existing Supercharged Ninja H2 and Ninja H2R machines are now joined by a third even more exclusive model in 2017, the Ninja H2 Carbon.
Kawasaki has created the Ninja ZX-10RR, a machine focused on podium potential and street exclusivity. With its RR logo on the right side engine cover, this machine is set apart from its peers.
For 2017 Kawasaki has fundamentally reviewed the overall Z1000SX package and refined both its sporting and touring prowess.
Kawasaki has unveiled the Ninja 650 for the 2017 model year, a twin cylinder, water-cooled machine targeting a wide spectrum of potential owners.
Kawasaki has announced a new colour option for the formidable Ninja ZX-10R for 2017, the machine currently dominating in the World Superbike Championship.
Kawasaki are offering a free Tourer Upgrade on the Versys 650 and Z1000SX for customers who buy between 23 June 2016 and 5 September 2016.


text:
Q: Converting numerical date back to date in Python I created a dataframe with the first column as 'date', the rest our numerical columns.
date    transportation  pm25    pm10    ozone   no2 so2 co  final_aqi   label
719163       21         162     193     24      40   2   16    193     119.0
....
589 rows × 10 columns

Here I converted the dates to numeric form using
import datetime as dt

df['date'] = pd.to_datetime(df['date'])

df['date'] = df['date'].map(dt.datetime.toordinal)

I wish to use linear regression on my code, will my predictions be altered if my date is numeric(date column is a feature)? Also how should I go back to the date format?

A: If date is not one of the input features, then there is no effect at all because it will not be included in your features matrix.
If date is an input to your model. i.e. one of the input features, then keep it numerical as it's.
If your model is predicting something that is related to a specific date, then it's better to include the distance from that date. i.e. (date - specific date)


text:
Dragon Ball Z: Kakarot Has Akira Toriyama's Seal of Approval, Teases Backstories Not in the Manga
Goku Won't Be the Only Playable Character in Dragon Ball Z: Kakarot
Dragon Ball Z: Kakarot Gameplay Video Has a Ton of Easter Eggs, Including a Forgotten Fan-Favorite!
Dragon Ball: Project Z is Now Dragon Ball Z: Kakarot, Comes Out in Early 2020
By Nico Parungo | More Articles Comic Book Fan and Lazy Gamer
Dragon Ball Z: Kakarot is going to be an interesting game for the franchise, one that seems to be solely focused on Son Goku. Fans are a bit worried since Goku is actually inactive during a large part of Z but the developers are promising a lot of stories some might not remember, along with some new ones. Dragon Ball creator Akira Toriyama decided to give his seal of approval for the game and told fans a couple of interesting tidbits.
On Twitter, Toriyama revealed that the game would have backstories for characters that weren't done in the manga. While most fans will see this as a cheap way to make filler seem exciting, the fact that fan-favorites like Launch and Android 8 aka Eighter are in the game shows that filler can still be entertaining.
We're excited to share a message from Dragon Ball creator Akira Toriyama about DRAGON BALL Z: KAKAROT! #DBZKAKAROT
Are you ready to experience this fresh new take on Goku's story? Pre-Order your copy today: https://t.co/9HQl4Brct8 pic.twitter.com/Q4zR6NX13D
— Bandai Namco US (@BandaiNamcoUS) June 24, 2019
Many of us are still hoping that we get more story details, specifically, what arc the game will end with. The trailers have made fans think that it will conclude with the Frieza Saga, which is baffling since those videos basically spoiled how that battle will go. They even showed Goku blasting Frieza with to smithereens while shouting the now iconic "YOU FOOL!"
Since previous interviews claimed that it would be Goku's story through all of Z, the game could end in the Buu Saga. After all, Goku has a lot of important battles there, from his long-awaited rematch with Vegeta to his epic fight with Kid Buu, there's just so much for the Saiyan to do. Please end with Buu.
Hell, end with Beerus. It's called Dragon Ball Z: Battle of Gods after all.
Dragon Ball Z: Kakarot is coming to PS4 and Xbox One in early 2020.
Read: Dragon Ball Z: Kakarot Gameplay Video Has a Ton of Easter Eggs, Including a Forgotten Fan-Favorite!
Nico Parungo graduated with a film degree from the De La Salle College of St. Benilde. He loves movies, comic books, video games and the WWE. He also likes to check out games and animated shows that are deemed underrated.
@Nico Parungo | parungonicolo@gmail.com
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text:
Página 13 dos resultados de 4394 itens digitais encontrados em 0.011 segundos
The National Academy of Sciences
The American Society of Human Genetics
Korean Society of Medical Biochemistry and Molecular Biology
Wiley Subscription Services, Inc., A Wiley Company
Mary Ann Liebert, Inc.
American Society for Biochemistry and Molecular Biology
MyJove Corporation
‣ Mutator phenotypes of yeast strains heterozygous for mutations in the MSH2 gene
Drotschmann, Karin; Clark, Alan B.; Tran, Hiep T.; Resnick, Michael A.; Gordenin, Dmitry A.; Kunkel, Thomas A.
Fonte: The National Academy of Sciences Publicador: The National Academy of Sciences
Publicado em 16/03/1999 Português
Heterozygosity for germ-line mutations in the DNA mismatch repair gene MSH2 predisposes humans to cancer. Here we use a highly sensitive reporter to describe a spontaneous mutator phenotype in diploid yeast cells containing a deletion of only one MSH2 allele. We also identify five MSH2 missense mutations that have dominant mutator effects in heterozygous cells when expressed at normal levels from the natural MSH2 promoter. For example, a 230-fold mutator effect is observed in an MSH2/msh2 diploid strain in which Gly693, which is invariant in MutS homologs and involved in ATP hydrolysis, is changed to alanine. DNA binding data suggest that mismatch repair is suppressed by binding of a mutant Msh2–Msh6 heterodimer to a mismatch with subsequent inability to dissociate from the mismatch in the presence of ATP. A dominant mutator effect also is observed in yeast when Gly693 is changed to serine. An early onset colorectal tumor is heterozygous for the analogous Gly → Ser mutation in hMSH2, and a second hMSH2 mutation was not found, suggesting that this missense mutation may predispose to cancer via a dominant mutator effect. The mutator effects of the deletion mutant and the Gly → Ala missense mutant in yeast MSH2 are enhanced by heterozygosity for a missense mutation in DNA polymerase δ that reduces its proofreading activity but is not a mutator in the heterozygous state. The synergistic effects of heterozygosity for mutations in two different genes that act in series to correct replication errors may be relevant to cancer predisposition.
‣ Saccharomyces cerevisiae pms2 mutations are alleles of MLH1, and pms2-2 corresponds to a hereditary nonpolyposis colorectal carcinoma-causing missense mutation.
Jeyaprakash, A; Das Gupta, R; Kolodner, R
Fonte: PubMed Publicador: PubMed
Publicado em /06/1996 Português
#Research Article
A number of mutant Saccharomyces cerevisiae strains having phenotypes consistent with defects in DNA mismatch repair have been described, but not all have been extensively characterized. In this study we demonstrate that the pms2-1 and pms2-2 alleles arise from missense mutations in the MLH1 gene which inactivate MLH1. One of these alleles, pms2-2, causes the same amino acid substitution in a highly conserved region of the known MutL homologs as that caused by a proposed missense mutation observed in a Swedish hereditary nonpolyposis colorectal carcinoma kindred. This observation supports the functional significance of missense mutations found in hereditary nonpolyposis colorectal carcinoma kindreds and indicates that in some cases S. cerevisiae can serve as a useful model system for the analysis of such mutations.
‣ Precise missense and silent point mutations are fixed in the genomes of poliovirus mutants from persistently infected cells.
Borzakian, S; Pelletier, I; Calvez, V; Colbere-Garapin, F
Poliovirus mutants selected in persistently infected human neuroblastoma cells have a modified cell tropism and can establish a secondary persistent infection in nonneural cells, such as HEp-2c cells. Nucleotide sequence analysis revealed that the genome of a persistent mutant, S11, differed from that of the parental lytic Sabin 1 poliovirus strain by 31 point mutations. Three mutations occurred in the noncoding regions. The other mutations resulted in 12 amino acid substitutions; 1 substitution occurred in a nonstructural protein (3A), while the other 11 substitutions were clustered in the capsid proteins VP2 and VP1. The same missense mutations, as well as many of the silent mutations that we observed in mutant S11, also accumulated in the genome of two other persistent viruses isolated from independent infections. This finding indicates that both missense and silent mutations are selected during the persistent infection of neuroblastoma cells and suggests that the secondary structure of RNA in the coding region may play a role in viral infection.
‣ Molecular basis of loss-of-function mutations in the glp-1 gene of Caenorhabditis elegans.
Kodoyianni, V; Maine, E M; Kimble, J
The glp-1 gene encodes a membrane protein required for inductive cell interactions during development of the nematode Caenorhabditis elegans. Here we report the molecular characterization of 15 loss-of-function (lf) mutations of glp-1. Two nonsense mutations appear to eliminate glp-1 activity; both truncate the glp-1 protein in its extracellular domain and have a strong loss-of-function phenotype. Twelve missense mutations and one in-frame deletion map to sites within the repeated motifs of the glp-1 protein (10 epidermal growth factor [EGF]-like and 3 LNG repeats extracellularly and 6 cdc10/SWI6, or ankyrin, repeats intracellularly). We find that all three types of repeated motifs are critical to glp-1 function, and two individual EGF-like repeats may have distinct functions. Intriguingly, all four missense mutations in one phenotypic class map to the N-terminal EGF-like repeats and all six missense mutations in a second phenotypic class reside in the intracellular cdc10/SWI6 repeats. These two clusters of mutations may identify functional domains within the glp-1 protein.
‣ Human MutL homolog (MLH1) function in DNA mismatch repair: a prospective screen for missense mutations in the ATPase domain
Ellison, Aaron R.; Lofing, Joan; Bitter, Grant A.
Fonte: Oxford University Press Publicador: Oxford University Press
390.2187%
#Articles
Germline mutations in the DNA mismatch repair (MMR) genes MSH2 and MLH1 are responsible for the majority of hereditary non-polyposis colorectal cancer (HNPCC), an autosomal-dominant early-onset cancer syndrome. Genetic testing of both MSH2 and MLH1 from individuals suspected of HNPCC has revealed a considerable number of missense codons, which are difficult to classify as either pathogenic mutations or silent polymorphisms. To identify novel MLH1 missense codons that impair MMR activity, a prospective genetic screen in the yeast Saccharomyces cerevisiae was developed. The screen utilized hybrid human-yeast MLH1 genes that encode proteins having regions of the yeast ATPase domain replaced by homologous regions from the human protein. These hybrid MLH1 proteins are functional in MMR in vivo in yeast. Mutagenized MLH1 fragments of the human coding region were synthesized by error-prone PCR and cloned directly in yeast by in vivo gap repair. The resulting yeast colonies, which constitute a library of hybrid MLH1 gene variants, were initially screened by semi-quantitative in vivo MMR assays. The hybrid MLH1 genes were recovered from yeast clones that exhibited a MMR defect and sequenced to identify alterations in the mutagenized region. This investigation identified 117 missense codons that conferred a 2-fold or greater decreased efficiency of MMR in subsequent quantitative MMR assays. Notably...
‣ Mutations in the Transmembrane Natriuretic Peptide Receptor NPR-B Impair Skeletal Growth and Cause Acromesomelic Dysplasia, Type Maroteaux
Bartels, Cynthia F.; Bükülmez, Hülya; Padayatti, Pius; Rhee, David K.; van Ravenswaaij-Arts, Conny; Pauli, Richard M.; Mundlos, Stefan; Chitayat, David; Shih, Ling-Yu; Al-Gazali, Lihadh I.; Kant, Sarina; Cole, Trevor; Morton, Jenny; Cormier-Daire, Val
Fonte: The American Society of Human Genetics Publicador: The American Society of Human Genetics
The homodimeric transmembrane receptor natriuretic peptide receptor B (NPR-B [also known as guanylate cyclase B, GC-B, and GUC2B]; gene name NPR2) produces cytoplasmic cyclic GMP from GTP on binding its extracellular ligand, C-type natriuretic peptide (CNP). CNP has previously been implicated in the regulation of skeletal growth in transgenic and knockout mice. The autosomal recessive skeletal dysplasia known as "acromesomelic dysplasia, type Maroteaux" (AMDM) maps to an interval that contains NPR2. We sequenced DNA from 21 families affected by AMDM and found 4 nonsense mutations, 4 frameshift mutations, 2 splice-site mutations, and 11 missense mutations. Molecular modeling was used to examine the putative protein change brought about by each missense mutation. Three missense mutations were tested in a functional assay and were found to have markedly deficient guanylyl cyclase activity. We also found that obligate carriers of NPR2 mutations have heights that are below the mean for matched controls. We conclude that, although NPR-B is expressed in a number of tissues, its major role is in the regulation of skeletal growth.
‣ Complete Loss of P/Q Calcium Channel Activity Caused by a CACNA1A Missense Mutation Carried by Patients with Episodic Ataxia Type 2
Guida, Serena; Trettel, Flavia; Pagnutti, Stefano; Mantuano, Elide; Tottene, Angelita; Veneziano, Liana; Fellin, Tommaso; Spadaro, Maria; Stauderman, Kenneth A.; Williams, Mark E.; Volsen, Stephen; Ophoff, Roel A.; Frants, Rune R.; Jodice, Carla; Frontali
#Report
Familial hemiplegic migraine, episodic ataxia type 2 (EA2), and spinocerebellar ataxia type 6 are allelic disorders of the CACNA1A gene (coding for the α1A subunit of P/Q calcium channels), usually associated with different types of mutations (missense, protein truncating, and expansion, respectively). However, the finding of expansion and missense mutations in patients with EA2 has blurred this genotype-phenotype correlation. We report the first functional analysis of a new missense mutation, associated with an EA2 phenotype—that is, T→C transition of nt 4747 in exon 28, predicted to change a highly conserved phenylalanine residue to a serine at codon 1491, located in the putative transmembrane segment S6 of domain III. Patch-clamp recording in HEK 293 cells, coexpressing the mutagenized human α1A-2 subunit, together with human β4 and α2δ subunits, showed that channel activity was completely abolished, although the mutated protein is expressed in the cell. These results indicate that a complete loss of P/Q channel function is the mechanism underlying EA2, whether due to truncating or to missense mutations.
‣ Missense and nonsense mutations in the lysosomal alpha-mannosidase gene (MANB) in severe and mild forms of alpha-mannosidosis.
Gotoda, Y; Wakamatsu, N; Kawai, H; Nishida, Y; Matsumoto, T
alpha-Mannosidosis is an autosomal recessive lysosomal-storage disorder caused by a deficiency of lysosomal alpha-mannosidase activity. This disease shows a wide range of clinical phenotypes, from a severe, infantile form (type I), which is fatal at <3-8 years of age, to a less severe, late-onset form (type II), which ultimately may involve hearing loss, coarse face, mental retardation, and hepatosplenomegaly. To elucidate the molecular mechanism underlying this disease in both types of patients, we have used PCR, followed by either SSCP analysis or direct sequencing, to analyze the 24 exons and intron/exon boundaries of the alpha-mannosidase gene (MANB) from five patients. Two amino acid substitutions-H72L and R750W, in exons 2 and 18, respectively-and two nonsense mutations-Q639X and R760X, in exons 15 and 19, respectively-were identified in four type II patients. One amino acid substitution, P356R, was identified in exon 8 from a type I patient. This patient and three of the type II patients were homozygous for their mutations (H72L, P356R, R750W, and R760X) and one type II patient was heterozygous for the Q639X and R750W mutations. Transfection experiments of COS 7 cells, using the alpha-mannosidase cDNA containing one of the missense mutations-H72L...
‣ Three different frameshift mutations of the tyrosinase gene in type IA oculocutaneous albinism.
Oetting, W S; Mentink, M M; Summers, C G; Lewis, R A; White, J G; King, R A
Mutations in the gene for the pigment-producing enzyme tyrosinase are responsible for type IA (tyrosinase-negative) oculocutaneous albinism (OCA). Most reported mutations have been single base substitutions. We now report three different frameshift mutations in three unrelated individuals with type IA OCA. The first individual has a single base deletion within a series of five guanidines, resulting in a premature stop codon in exon I on one allele and a missense mutation at codon 382 in exon III on the homologous allele. The second individual is a genetic compound of two separate frameshift mutations, including both the same exon I single base deletion found in the first individual and a deletion of a thymidine-guanidine pair, within the sequence GTGTG, forming a termination codon (TAG) in exon I on the homologous allele. The third individual has a single base insertion in exon I on one allele and a missense mutation at codon 373 in exon III on the homologous allele. The two missense mutations occur within the copper Bbinding region and may interfere with either copper binding to the enzyme or oxygen binding to the copper. These five different mutations disrupt tyrosinase function and are associated with a total lack of melanin biosynthesis.
‣ Defective cellular trafficking of missense NPR-B mutants is the major mechanism underlying acromesomelic dysplasia-type Maroteaux
Hume, Alistair N.; Buttgereit, Jens; Al-Awadhi, Aydah M.; Al-Suwaidi, Sarah S.; John, Anne; Bader, Michael; Seabra, Miguel C.; Al-Gazali, Lihadh; Ali, Bassam R.
Natriuretic peptides (NPs) comprise a family of structurally related but genetically distinct hormones that regulate a variety of physiological processes such as cardiac growth, blood pressure, axonal pathfinding and endochondral ossification leading to the formation of vertebrae and long bones. The biological actions of NPs are mediated by natriuretic peptide receptors (NPRs) A, B and C that are located on the cell surface. Mutations in NPR-B have been shown to cause acromesomelic dysplasia-type Maroteaux (AMDM), a growth disorder in humans and severe dwarfism in mice. We hypothesized that missense mutations of NPR-B associated with AMDM primarily affect NPR-B function by the arrest of receptor trafficking at the endoplasmic reticulum (ER), due to conformational change, rather than an impairment of ligand binding, transmission of signal through the membrane or catalytic activity. Twelve missense mutations found in AMDM patients and cn/cn mice were generated by site-directed mutagenesis and transiently overexpressed in HeLa cells. Confocal microscopy revealed that 11 out of 12 mutants were retained in the ER. Determination of the ligand-dependent cGMP response confirmed that ER-retained NPR-B mutants are non-functional. Meanwhile, the only cell surface-targeted NPR-B missense mutant (D176E) displayed greatly reduced enzymatic activity due to impaired ligand binding. Thus...
‣ Infection-Triggered Familial or Recurrent Cases of Acute Necrotizing Encephalopathy Caused by Mutations in a Component of the Nuclear Pore, RANBP2
Neilson, Derek E.; Adams, Mark D.; Orr, Caitlin M.D.; Schelling, Deborah K.; Eiben, Robert M.; Kerr, Douglas S.; Anderson, Jane; Bassuk, Alexander G.; Bye, Ann M.; Childs, Anne-Marie; Clarke, Antonia; Crow, Yanick J.; Di Rocco, Maja; Dohna-Schwake, Christ
Fonte: American Society of Human Genetics Publicador: American Society of Human Genetics
Acute necrotizing encephalopathy (ANE) is a rapidly progressive encephalopathy that can occur in otherwise healthy children after common viral infections such as influenza and parainfluenza. Most ANE is sporadic and nonrecurrent (isolated ANE). However, we identified a 7 Mb interval containing a susceptibility locus (ANE1) in a family segregating recurrent ANE as an incompletely penetrant, autosomal-dominant trait. We now report that all affected individuals and obligate carriers in this family are heterozygous for a missense mutation (c.1880C→T, p.Thr585Met) in the gene encoding the nuclear pore protein Ran Binding Protein 2 (RANBP2). To determine whether this mutation is the susceptibility allele, we screened controls and other patients with ANE who are unrelated to the index family. Patients from 9 of 15 additional kindreds with familial or recurrent ANE had the identical mutation. It arose de novo in two families and independently in several other families. Two other patients with familial ANE had different RANBP2 missense mutations that altered conserved residues. None of the three RANBP2 missense mutations were found in 19 patients with isolated ANE or in unaffected controls. We conclude that missense mutations in RANBP2 are susceptibility alleles for familial and recurrent cases of ANE.
‣ Effects of a chemical chaperone on genetic mutations in α-galactosidase A in Korean patients with Fabry disease
Park, Jung-Young; Kim, Gu-Hwan; Kim, Sung-Su; Ko, Jung Min; Lee, Jin-Joo; Yoo, Han-Wook
Fonte: Korean Society of Medical Biochemistry and Molecular Biology Publicador: Korean Society of Medical Biochemistry and Molecular Biology
#Original Article
Fabry disease is an X-linked inborn error of glycosphingolipid catabolism that results from mutations in the gene encoding the α-galactosidase A (GLA) enzyme. We have identified 15 distinct mutations in the GLA gene in 13 unrelated patients with classic Fabry disease and 2 unrelated patients with atypical Fabry disease. Two of the identified mutations were novel (i.e., the D231G missense mutation and the L268delfsX1 deletion mutation). This study evaluated the effects of the chemical chaperones 1-deoxygalactonojirimycin (DGJ) on the function of GLA in vitro, in cells containing missense mutations in the GLA gene. Nine missense and a nonsense mutations, including one novel mutation were cloned into mammalian expression vectors. After transient expression in COS-7 cells, GLA enzyme activity and protein expression were analyzed using fluorescence spectrophotometry and Western blot analysis, respectively. DGJ enhanced GLA enzyme activity in the M42V, I91T, R112C and F113L mutants. Interestingly, the I91T and F113L mutations are associated with the atypical form of Fabry disease. However, DGJ treatment did not have any significant effect on the GLA enzyme activity and protein expression of other mutants, including C142W, D231G, D266N, and S297F. Of note...
‣ Lynch Syndrome-associated Mutations in MSH2 Alter DNA Repair and Checkpoint Response Functions In Vivo
Mastrocola, Adam S; Heinen, Christopher D
Fonte: Wiley Subscription Services, Inc., A Wiley Company Publicador: Wiley Subscription Services, Inc., A Wiley Company
#Mutation in Brief
The DNA mismatch repair (MMR) pathway is essential in maintaining genomic stability through its role in DNA repair and the checkpoint response. Loss of DNA MMR underlies the hereditary cancer disease Lynch Syndrome (LS). Germline mutations in MSH2 account for approximately 40% of LS patients and of these, 18% are missense variants. One important clinical challenge has been discriminating between missense variants that are pathogenic and those that are not. Current analysis of missense mutations in MSH2 is performed using a combination of clinical, biochemical, and functional data; however, suitable cell culture models to test the various functions of the DNA MMR proteins are lacking. Here, we have generated human cell lines stably expressing a subset of MSH2 missense mutants and tested their effect on DNA repair and checkpoint response functions. We have expanded on previous biochemical and functional analyses performed in non-human systems to further understand defects conferred by this subset of single amino acid alterations. The functional characterization of MSH2 missense mutants combined with clinical and biochemical data is essential for appropriate patient management and genetic counseling decisions. ©2010 Wiley-Liss, Inc.
‣ Fibulin-5 mutations link inherited neuropathies, age-related macular degeneration and hyperelastic skin
Auer-Grumbach, Michaela; Weger, Martin; Fink-Puches, Regina; Papić, Lea; Fröhlich, Eleonore; Auer-Grumbach, Piet; El Shabrawi-Caelen, Laila; Schabhüttl, Maria; Windpassinger, Christian; Senderek, Jan; Budka, Herbert; Trajanoski, Slave; Janecke, Andreas
To identify the disease-causing gene responsible for an autosomal dominantly inherited Charcot–Marie–Tooth neuropathy subtype in a family excluded for mutations in the common Charcot–Marie–Tooth genes, we used array-based sequence capture to simultaneously analyse the disease-linked protein coding exome at chromosome 14q32. A missense mutation in fibulin-5, encoding a widely expressed constituent of the extracellular matrix that has an essential role in elastic fibre assembly and has been shown to cause cutis laxa, was detected as the only novel non-synonymous sequence variant within the disease interval. Screening of 112 index probands with unclassified Charcot–Marie–Tooth neuropathies detected two further fibulin-5 missense mutations in two families with Charcot–Marie–Tooth disease and hyperextensible skin. Since fibulin-5 mutations have been described in patients with age-related macular degeneration, an additional 300 probands with exudative age-related macular degeneration were included in this study. Two further fibulin-5 missense mutations were identified in six patients. A mild to severe peripheral neuropathy was detected in the majority of patients with age-related macular degeneration carrying mutations in fibulin-5. This study identifies fibulin-5 as a gene involved in Charcot–Marie–Tooth neuropathies and reveals heterozygous fibulin-5 mutations in 2% of our patients with age-related macular degeneration. Furthermore...
‣ Identification of Two Novel Mutations of the HOMEZ Gene in Chinese Patients with Isolated Ventricular Septal Defect
Xuan, Chao; Jia, Ke-Gang; Wang, Bin-Bin; Bai, Xiao-Yan; Gao, Ge; Yang, Qin; Wang, Xiu-Li; Liu, Xiao-Cheng; Ma, Xu; He, Guo-Wei
Fonte: Mary Ann Liebert, Inc. Publicador: Mary Ann Liebert, Inc.
#Original Articles
Objectives: Ventricular septal defect (VSD) is the most common congenital heart disease (CHD). Genome-wide linkage analysis revealed a potential CHD susceptibility locus in the homeodomain leucine zipper-encoding (HOMEZ) gene in a South Indian population. The present study aimed to identify potential pathogenic mutations for HOMEZ and to provide insights into the etiology of isolated VSD in the Chinese population. Methods: Case–control mutational analysis was performed in 400 patients with isolated VSD and 400 healthy controls. Protein-coding exton of HOMEZ and their flanking sequences were amplified by polymerase chain reaction and sequenced on an ABI3730 Automated Sequencer. CLC workbench software was used to compare the conservatism of the HOMEZ protein with other multiple species. The ExPASy-ProtScale online tool was used to predicate the alignment of the hydrophobic features. Results: Two novel heterozygous missense mutations (c.116 C>T; c. 630T>A) were identified in HOMEZ gene exon-2. The two mutations lead to alanine to valine substitution at position 39 and serine to arginine at position 210, which are highly conserved among many species. The hydropathicity of the valine and arginine residue at the position 39 and 210 were significantly different from the wild type. Conclusions: We have identified two novel heterozygous missense mutations in HOMEZ gene exon-2 in isolated VSD patients in the Chinese population and have found that these two mutations resulted in alteration of the hydropathicity of the HOMEZ protein. Therefore...
‣ Investigation of Endoglin Wild-Type and Missense Mutant Protein Heterodimerisation Using Fluorescence Microscopy Based IF, BiFC and FRET Analyses
Förg, Tassilo; Hafner, Mathias; Lux, Andreas
Fonte: Public Library of Science Publicador: Public Library of Science
The homodimeric transmembrane receptor endoglin (CD105) plays an important role in angiogenesis. This is highlighted by mutations in its gene, causing the vascular disorder HHT1. The main role of endoglin function has been assigned to the modulation of transforming growth factor β and bone morphogenetic protein signalling in endothelial cells. Nevertheless, other functions of endoglin have been revealed to be involved in different cellular functions and in other cell types than endothelial cells. Compared to the exploration of its natural function, little experimental data have been gathered about the mode of action of endoglin HHT mutations at the cellular level, especially missense mutations, and to what degree these might interfere with normal endoglin function. In this paper, we have used fluorescence-based microscopic techniques, such as bimolecular fluorescence complementation (BiFC), immunofluorescence staining with the endoglin specific monoclonal antibody SN6, and protein interaction studies by Förster Resonance Energy Transfer (FRET) to investigate the formation and cellular localisation of possible homo- and heterodimers composed of endoglin wild-type and endoglin missense mutant proteins. The results show that all of the investigated missense mutants dimerise with themselves...
‣ Naturally Occurring Mutations Alter the Stability of Polycystin-1 Polycystic Kidney Disease (PKD) Domains*
Ma, Liang; Xu, Meixiang; Forman, Julia R.; Clarke, Jane; Oberhauser, Andres F.
Fonte: American Society for Biochemistry and Molecular Biology Publicador: American Society for Biochemistry and Molecular Biology
#Protein Structure and Folding
Mutations in polycystin-1 (PC1) can cause autosomal dominant polycystic kidney disease, which is a leading cause of renal failure. The available evidence suggests that PC1 acts as a mechanosensor, receiving signals from the primary cilia, neighboring cells, and extracellular matrix. PC1 is a large membrane protein that has a long N-terminal extracellular region (about 3000 amino acids) with a multimodular structure including 16 Ig-like polycystic kidney disease (PKD) domains, which are targeted by many naturally occurring missense mutations. Nothing is known about the effects of these mutations on the biophysical properties of PKD domains. Here we investigate the effects of several naturally occurring mutations on the mechanical stability of the first PKD domain of human PC1 (HuPKDd1). We found that several missense mutations alter the mechanical unfolding pathways of HuPKDd1, resulting in distinct mechanical phenotypes. Moreover, we found that these mutations also alter the thermodynamic stability of a structurally homologous archaeal PKD domain. Based on these findings, we hypothesize that missense mutations may cause autosomal dominant polycystic kidney disease by altering the stability of the PC1 ectodomain, thereby perturbing its ability to sense mechanical signals.
‣ Toward Classification of BRCA1 Missense Variants Using a Biophysical Approach
Rowling, Pamela J. E.; Cook, Rebecca; Itzhaki, Laura S.
Carriers of germ line mutations in breast cancer susceptibility gene BRCA1 have an increased risk of developing breast and ovarian cancers; missense mutations have, however, been difficult to assess for disease association. Here we have used a biophysical approach to classify these variants. We established an assay for measuring the thermodynamic stability of the BRCA1 BRCT domains and investigated the effects of 36 missense mutations. The mutations show a range of effects. Some do not change the stability, whereas others destabilize the protein by as much as 6 kcal mol−1; one-third of the mutants could not be expressed in soluble form in Escherichia coli, and we conclude that these destabilize the protein by an even greater amount. We tested several computer algorithms for their ability to predict the mutant effects and found that by grouping them into two classes (destabilizing by less than or more than 2.2 kcal mol−1), the algorithms could predict the stability changes. Importantly, with the exception of the few mutants located in the binding site, none showed a significant reduction in affinity for phosphorylated substrate. These results indicate that despite very large losses in stability, the integrity of the structure is not compromised by the mutations. Thus...
‣ Seven novel mutations in the methylenetetrahydrofolate reductase gene and genotype/phenotype correlations in severe methylenetetrahydrofolate reductase deficiency.
Goyette, P; Frosst, P; Rosenblatt, D S; Rozen, R
5-Methyltetrahydrofolate, the major form of folate in plasma, is a carbon donor for the remethylation of homocysteine to methionine. This form of folate is generated from 5,10-methylenetetrahydrofolate through the action of 5,10-methylenetetrahydrofolate reductase (MTHFR), a cytosolic flavoprotein. Patients with an autosomal recessive severe deficiency of MTHFR have homocystinuria and a wide range of neurological and vascular disturbances. We have recently described the isolation of a cDNA for MTHFR and the identification of two mutations in patients with severe MTHFR deficiency. We report here the characterization of seven novel mutations in this gene: six missense mutations and a 5' splice-site defect that activates a cryptic splice site in the coding sequence. We also present a preliminary analysis of the relationship between genotype and phenotype for all nine mutations identified thus far in this gene. A nonsense mutation and two missense mutations (proline to leucine and threonine to methionine) in the homozygous state are associated with extremely low activity (0%-3%) and onset of symptoms within the 1st year of age. Other missense mutations (arginine to cysteine and arginine to glutamine) are associated with higher enzyme activity and later onset of symptoms.
‣ Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1
Parvin, Jeffrey; Chiba, Natsuko; Ransburgh, Derek
Fonte: MyJove Corporation Publicador: MyJove Corporation
#Cell Biology
The functional analysis of missense mutations can be complicated by the presence in the cell of the endogenous protein. Structure-function analyses of the BRCA1 have been complicated by the lack of a robust assay for the full length BRCA1 protein and the difficulties inherent in working with cell lines that express hypomorphic BRCA1 protein1,2,3,4,5. We developed a system whereby the endogenous BRCA1 protein in a cell was acutely depleted by RNAi targeting the 3'-UTR of the BRCA1 mRNA and replaced by co-transfecting a plasmid expressing a BRCA1 variant. One advantage of this procedure is that the acute silencing of BRCA1 and simultaneous replacement allow the cells to grow without secondary mutations or adaptations that might arise over time to compensate for the loss of BRCA1 function. This depletion and add-back procedure was done in a HeLa-derived cell line that was readily assayed for homologous recombination activity. The homologous recombination assay is based on a previously published method whereby a recombination substrate is integrated into the genome (Figure 1)6,7,8,9. This recombination substrate has the rare-cutting I-SceI restriction enzyme site inside an inactive GFP allele, and downstream is a second inactive GFP allele. Transfection of the plasmid that expresses I-SceI results in a double-stranded break...


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"Our Constitution, by including environmental rights as fundamental, justiciable human rights, by necessary implication requires that environmental considerations be accorded appropriate recognition and respect in the administrative processes in our country. Together with the change in the ideological climate must also come a change in our legal and administrative approach to environmental concerns." (Director: Mineral Development, Gauteng Region v Save the Vaal Environment)
The constitutionally protected environmental right has increased the awareness of environmental law in every aspect of decision making which might have an impact on the environment. Companies' operating within the South African environmental sphere have an obligation to adhere to the legal obligations, norms, standards, principles and guidelines as contained in South Africa's multifaceted network of environmental legislation. Stemming from the vast array of environmental legislation that companies have to contend with, almost on a daily basis, it has become vital for companies to obtain strategic environmental legal advice in this unique field.
Legislation which may be applicable to companies includes, but is not limited to: Constitution of the Republic of South Africa, 1996 (Act No. 108 of 1996); National Environmental Management Act, 1998 (Act No. 107 of 1998);
National Water Act, 1998 (Act No. 36 of 1998);
Environment Conservation Act, 1989 (Act No. 73 of 1989);
Mineral and Petroleum Resources Development Act, 2002 (Act No. 28 of 2002); Hazardous Substances Act, 1973 (Act No. 15 of 1973);
Conservation of Agricultural Resources Act, 1983 (Act No. 43 of 1983); National Heritage Resources Act, 1999 (Act No. 25 of 1999);
National Environmental Management: Biodiversity Act, 2004 (Act No. 10 of 2004);
National Environmental Management: Protected Areas Act, 2003 (Act No. 57 of 2003);
National Environmental Management: Air Quality Act, 2004 (Act No. 39 of 2004); Atmospheric Pollution Prevention Act, 1965 (Act No. 45 of 1965);
Mine Health and Safety Act, 1996 (Act No. 29 of 1996);
Occupational Health and Safety Act, 1993 (Act No. 85 of 1993); and
Regulations, Ordinances and Bylaws.
GCS can provide the following services to enable companies to legally operate their activities in an environmentally sound manner: Environmental and Occupational Health and Safety Legal Compliance Audits.
Assessment of environmental legislative compliance and authorisation investigations relevant to new project developments.
Assisting clients with the identification of environmental legal risks and liabilities during the planning and pre-feasibility,
feasibility, operational and closure stages of projects. Due diligence investigations.
Legal Review for Scoping documents and Environmental Impact Assessment Report.
General environmental legal advice and legal assistance.
Monitoring and updates of legal developments and the opportunity to provide input into legislative and policy developments if applicable.
Assessment of the environmental legislative framework for African countries.
Environmental Legal Compliance Audits for African countries.
GCSAdmin / Posted on 13 March 2018 13 October 2020 / 0


I finally took Mark's advice and grabbed up some Chroma Atelier Interactive paint.
I was very pleased with the blending and work-ability of this paint.
Though I have much more to explore, thought I would share my first painting with this paint and hopefully show some of the techniques Mark has taught me.


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Both Dallas, Seattle Riding Crazy 14-1 Streaks
The Cowboys have won 14 of their last 15 games in Week 3 of the season, dating back to the 2003. Technically, a couple of the wins have occurred in the following week, but because of a bye week, the Cowboys have still won all but one of their last 15 in the third game of the season.
2017: Cowboys 28, Cardinals 17: In a game overshadowed by pre-game protests with the Cowboys uniting together as an organization before the kickoff, the Cowboys made some second-half plays to rally past the Cardinals, thanks to long plays from Brice Butler, who signed with the Cardinals this offseason but is now back with the Cowboys as of this week.
2016: Cowboys 31, Bears 17: It wasn't just a coming-out party for Ezekiel Elliott, but a going-up game as well, as he showed off his leaping ability, along with the running skills that made him the No. 4 overall pick.
2015: Falcons 39, Cowboys 28: Brandon Weeded took over for an injured Tony Romo and looked to have the offense rolling in the first half with four touchdowns, including three by Joseph Randle. But Julio Jones and the Falcons were too strong in the second half and rolled to the only victory by a Cowboys opponent in Week 3 since 2002.
2014: Cowboys 34, Rams 31: In one of the largest comebacks in Cowboys history, Dallas came back from a 21-0 hole to rally for a tough road win. In what turned into an offensive shootout, the defense came on strong late, with two interceptions, including a game-clinching play from Morris Claiborne.
2013: Cowboys 31, Rams 7: DeMarco Murray just loved playing the Rams. He not only set the franchise single-record vs. St Louis two years earlier with 253 yards, but rushed for 175 in this Week 3 victory.
2012: Cowboys 16, Bucs 10: In a defensive struggle, the Cowboys' best defender was a difference maker as DeMarcus Ware led the team with two sacks in the low-scoring affair.
2011: Cowboys 18, Redskins 16: Despite not scoring a single touchdown, the Cowboys were able to survive the Redskins, thanks to a rookie kicker named Dan Bailey, who accounted for all the points with six field goals.
2010: Cowboys 27, Texans 13: An 0-2 start to the season didn't affect the Cowboys, who found Texas Longhorn Roy Williams on two long touchdowns to spark a 14-point win.
2009: Cowboys 21, Panthers 7: After losing the first-ever game in AT&T Stadium in Week 2, the Cowboys came back the next Monday night and shut down the Panthers for the first NFL victory in Arlington.
2008: Cowboys 27, Packers 16: Before blowing up in Kansas City the next year, Miles Austin had his first real breakout moment with two big plays in Green Bay as the Cowboys went to 3-0.
2007: Cowboys 34, Bears 10: A close game in the third quarter, the Cowboys scored the final 24 points, including a second TD run from Marion Barber, along with Anthony Henry's Pick-6.
2006: Cowboys 45, Titans 14: There were plenty of storylines before, during and after this game. From T.O., to Andre Gurode to playing Vince Young in his debut, the Cowboys rolled past Tennessee for an easy win.
2005: Cowboys 34, 49ers 31: A few of Bill Parcells' former players in other spots put it together when it counted the most as Drew Bledsoe, Terry Glenn and Keyshawn Johnson sparked the Cowboys for an overtime victory.
2004: Cowboys 21, Redskins 18: Veteran Vinny Testaverde led the Cowboys to an upset road win, but he wasn't the only one throwing TDs and veteran fullback Richie Anderson shocked Washington with a TD throw.
2003: Cowboys 17, Jets 6: Bill Parcells got his second victory in New York in a row, as the Cowboys had already edged the Giants in Week 2, but then steamrolled the Jets on the road the next game.
Rank'Em: Parsons Leads Top 10 Cowboys vs. Saints
The Cowboys used a team effort to get past the Saints on Thursday but who stood out the most? Check out this week's Top 10 standouts from the win in New Orleans.
"Dressed For Success" Cowboys vs 49ers | 2021
Views of the Dallas Cowboys "Dressed for Success" as they host the San Francisco 49ers in a Wild Card playoff game at AT&T Stadium, presented by Jack Black.
Wild Card Round: Cowboys vs 49ers | 2021
Views of the NFC Wild Card matchup between the Dallas Cowboys and the San Francisco 49ers at AT&T Stadium.


The woman sits in a park, legs crossed, smoking, relaxed. Two men watch her from a window above the park, and they discuss her, looking down on her. "If she were six, you would say she was sulky," one of the men says. "Since her incarceration, her group of friends has been destroyed." She is meeting the two men, and one says, "She won't come in a moment before it's time." It is an eloquent exchange, and even more eloquently filmed, the woman seen in long shot, from above. She knows she is being watched. Her "relaxation," then, is an act of public theatre.
This is the opening scene in Christian Petzold's effective psychological and political thriller "Barbara," and it sets up the tension and atmosphere with precision and a taut elegance. It is East Germany, 1980. Barbara, the woman, played by Petzold's regular muse, Nina Hoss, is a chilly alert blonde. Barbara is a doctor and has been placed in a rural medical clinic as punishment for applying for an exit visa. Given an apartment by the State, Barbara is under constant surveillance by the Stasi. Andre, the doctor who runs the clinic (played by the gentle, hunky Ronald Zehrfeld), is an informer for the Stasi, and yet he does not seem devoted or fanatical in that role. His primary priority is his patients. He would also like to get to The Hague someday, to see Rembrandt's "Anatomy Lesson" in person, although his hope for being allowed such a trip is dim.
Andre recognizes Barbara's gift at her job during her first shift, when she correctly diagnoses a teenage girl (Jasna Fritzi Bauer) with meningitis, the signs of which the rest of the doctors had missed. Barbara, the cool character self-consciously smoking in the park, is an entirely different person at her job. The girl has escaped from Torgau, a Socialist work camp nearby, and Barbara treats her with a mischievous warm camaraderie, and reads to her at night. Barbara's choice of book is significant: The Adventures of Huckleberry Finn.
In Mark Twain's classic, Huck and Jim, on the run, take to the Mississippi River in a raft, loosening the fetters that bind them on the mainland, all while plunging deeper and deeper into slave country. Barbara, who has secret rendezvous in a nearby forest with a lover from West Germany, smokes expensive black market cigarettes, and has mysterious meetings with people who surreptitiously hand her packets of money, is a flight risk: you can sense that in her tense posture, and in her wild-looking eyes flicking out the window at the sound of every car horn.
Nina Hoss is a magnificent actress, watchful, tense, and spikily intelligent. When a smile cracks open on her face, the warmth and beauty is heartbreaking, because of the contrast. Hoss' work in all of Petzold's films is good, and while he has used her continuously, she never shows up as quite the same character. The woman in "Yella" is not the same as the one in "Jerichow" or in "Barbara." Nina Hoss can do anything.
In East Germany, in 1980, no one is to be trusted, and Petzold creates that sense of oppression with meticulous sound design (never has a doorbell sounded so frightening), and yawning gaps in between conversations, gaps filled with sound: wind, echoing footsteps in a hallway, trees rustling, the crunch of gravel under bicycle wheels. There is no score. The only music comes from the radio, once, and when Barbara plays her newly-tuned piano alone in her apartment. Barbara's landlady stares at her with cold expressionless eyes, a piano tuner shows up unexpectedly, freaking Barbara out (he must be there to inform on her), and every interpersonal interaction is charged with suspicion. Even the romantic and sexual tension in between Barbara and Andre seems loaded and dangerous.
"Barbara" is extremely suspenseful, without using the common genre trappings. The workings of the clinic are observed in a detailed fashion. Andre has set up a lab where he makes his own serum, a fact that impresses Barbara, accustomed as she is to better big-city hospitals. Petzold's films with Hoss are often about different sub-cultures of work, as well as about the long shadow left by Germany's split: in "Yella," Petzold examines the risky thrill-seeking world of corporate negotiations; in "Jerichow" it's the ins and outs of snack bar ownership, and in "Barbara," the challenges of running an out-of-the-way clinic, short on supplies and staff. There are decisions to be made about the patients (the teenage girl with meningitis, and another teenager, a failed suicide), and these decisions require cooperation and openness, anathema in a police state.
Andre, after examining the boy who attempted suicide, confides to Barbara, "I don't know. Something is wrong. I can feel it." One could say that about the entirety of the world portrayed in "Barbara," a world filled with everyday objects: bathtubs, pianos, bicycles, cigarettes, all of which take on portentous significance when seen in the context of totalitarian culture. Something is wrong in East Germany, and everyone can feel it. Doctors examine a patient and make a guess at a diagnosis.
The reality of Barbara's world, and the world of all East Germans, is one of constant surveillance, omnipresent informers, and a huge gap between private and public behavior. Petzold is a master at creating the kind of tension that can be felt on a subterranean level, a sort of acute uneasiness that can't be easily diagnosed, fixed, or even acknowledged by the characters. This is well-trod ground for Petzold, but never has it been so fully realized, so palpable, as in "Barbara."


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Home » Contact Us » Driving Directions
Truck Delivery Directions To Jamestown Electro Plating
From The North (Buffalo NY) – Follow 90 West to Dunkirk/Fredonia exit (exit 59). After exiting toll booths, turn left onto route 60 South. Follow route 60 to Gerry (approximately 19 miles). In Gerry, turn slight right to stay on route 60 South. Continue to follow route 60 South to Jamestown (approximately 6 miles). After you reach Jamestown, follow route 60 into town. Once you reach Sixth Street, turn right and follow sixth street for a few blocks until you reach a street light at Washington Street. Turn left onto Washington Street and proceed down the hill and across the Washington Street bridge. After the bridge, Washington Street will bend to the left. Approximately 200 yards after the second street light (at Main Street), there will be a turn to the left onto Institute and then a quick turn to the right onto Allen Street. Follow Allen Street by WCA Hospital to the Stop sign. Turn slightly to the left and continue on Allen Street until the next Stop sign (at The 7-Eleven). Turn slightly to the left again and follow Winsor Street one block to Water Street. Turn left onto Water Street. Follow Water Street around the curve to the left and at the next curve (to the right), Jamestown Electro Plating will be on the right.
From The West (Erie PA) – Follow route 86 East to Jamestown Exit (Exit 12). At the bottom of the off-ramp turn right and follow route 60 into Jamestown. Once you reach Sixth Street, turn right and follow sixth street for a few blocks until you reach a street light at Washington Street. Turn left onto Washington Street and proceed down the hill and across the Washington Street bridge. After the bridge, Washington Street will bend to the left. Approximately 200 yards after the second street light (at Main Street), there will be a turn to the left onto Institute and then a quick turn to the right onto Allen Street. Follow Allen Street by WCA Hospital to the Stop sign. Turn slightly to the left and continue on Allen Street until the next Stop sign (at The 7-Eleven). Turn slightly to the left again and follow Winsor Street one block to Water Street. Turn left onto Water Street. Follow Water Street around the curve to the left and at the next curve (to the right), Jamestown Electro Plating will be on the right.
From The East (Olean) – Follow route 86 West to Jamestown Exit (Exit 12). At the end of the off-ramp, turn left and follow route 60 into Jamestown. Once you reach Sixth Street, turn right and follow sixth street for a few blocks until you reach a street light at Washington Street. Turn left onto Washington Street and proceed down the hill and across the Washington Street bridge. After the bridge, Washington Street will bend to the left. Approximately 200 yards after the second street light (at Main Street), there will be a turn to the left onto Institute and then a quick turn to the right onto Allen Street. Follow Allen Street by WCA Hospital to the Stop sign. Turn slightly to the left and continue on Allen Street until the next Stop sign (at The 7-Eleven). Turn slightly to the left again and follow Winsor Street one block to Water Street. Turn left onto Water Street. Follow Water Street around the curve to the left and at the next curve (to the right), Jamestown Electro Plating will be on the right.
From The South (Warren) – From Warren, follow route 62 North into New York. A few miles after you enter New York, you will come to a street light at a T in the road. Turn left onto route 60 North and follow until you reach Jamestown. Once you reach the Jamestown city limits, continue to follow route 60 past McDonalds and proceed down the hill. After McDonalds, you will go through a light at the intersection of Newland Avenue and then turn slight right onto Foote Avenue. Follow Foote Avenue past WCA Hospital and through the light at the intersection of Allen Street. Shortly after Allen street, turn right onto Water Street. Follow Water Street around the curve to the left and Jamestown Electro Plating will be on the left.