text:
Column: A Democratic convention without convening that succeeds
I have been to several presidential nominating conventions. My first was Bill Clinton's in Madison Square Garden in 1992. The convening itself — the drawing together of the party's faithful, the diehards, the sizzle of their excitement — created the spectacle and the specialness.
A part-political tent revival, part-cult congregational, lavish party.
For that reason, it was hard to conceive of a virtual convention, dictated by social distancing as a deadly virus still rages. Indeed, as the Democratic National Convention opened Monday night, I feared that, despite all their efforts, the convention would fail to succeed.
There was a particular charge and effectiveness of seeing people in situ, surrounded by their books, in their kitchens, on their lawn, in some place that is meaningful to them or the people they represent.
The lack of a live audience also stripped away a bit of the performative nature of speeches and presentations; no pausing for applause, no way to know for certain if a line was landing. You simply had to deliver your speech.
But yet another benefit was that because there was no roar of the crowd, everyone could be clearly heard; because there was no crowd, cameras stayed fixed on the speaker instead of panning to the audience.
Some of the features of this convention should actually be preserved and repeated, even after we can meet again in large gatherings.
But the Democrats' message and their inclusiveness, not the format, have been the real stars of the convention. They have served to remind America of the beauty of its diversity and to encourage the continued quest for universal equality.
This has not by any means been without hiccups. As Julián Castro, former Democratic presidential candidate and former secretary of housing and urban development, told NPR:
''You know, last week I saw the schedule. And out of the 35 prime-time speakers, only three of them were Latino. There were no Native Americans, no Muslim Americans. And I said that that was — I didn't think that completely reflected this beautiful, diverse coalition, this big tent that Democrats have put together over these last few years. However, over the weekend, they did make some good announcements of additional speakers, including Latinas and Latinos and Native American and Muslim American speakers. So that's a positive. And more important than, you know, the words is the actions.''
Further, some progressives have not been satisfied with their wing of the party's level of representation at the convention, and many were not happy when Joe Biden said on the opening night of the convention that ''most cops are good, but the fact is, the bad ones need to be identified and prosecuted.''
Police defenders often offer up the ''bad apples'' theory on police misconduct, which implicitly suggests that our current system of policing is fine and functioning, and simply in need of a weeding. Many progressives take the opposite view: that the system of the police itself is corrupt, defiled, misdirected and bloated, and must be dealt with as such.
Still, all in all, the Democrats' effort is succeeding and is a welcome note of positivity before what surely will be a Republican convention filled with fearmongering and divisiveness.
As CNN and others reported Wednesday, Patricia and Mark McCloskey, the St. Louis homeowners who pointed guns at protesters, are scheduled to speak at the convention.
It is already clear that the selection of Kamala Harris as Biden's running mate and the blistering indictment of President Donald Trump delivered by Michelle Obama have gotten under Trump's skin. That has commanded the headlines, these two Black women pointing out his flaws and calling him a failure. This is something Trump can't abide. He will want a grander spectacle with even more fight.
He will want to divert attention from that which Democrats have continued to highlight: We are in a pandemic in which more than 170,000 Americans have died, mainly because of Trump's own colossal mismanagement of this country's response, and thousands more are expected to die.
For me, the Democratic convention so far has been a welcome reprieve from a cascade of lies, disinformation, bullying, blaming and bragging.
The greatest thing the DNC might well achieve, even in its abnormal format, is to remind us of what normalcy feels like.
Charles M. Blow writes for The New York Times. newsservice@nytimes.com


We're always cold too. This is a knit, pullover jacket with a quarter-length front zipper, raglan sleeves and one zippered sleeve pocket. 10-oz 100% polyester knitted fleece dyed with a low-impact process that significantly reduces the use of dyestuffs, energy and water compared to conventional dyeing methods. Fabric is certified as bluesign approved. Fair Trade Certified sewing. Patagonia is the holy grail, started-it-all Sustainability Guru. In the '90s they were the first outdoor clothing brand to manufacture fabric from recycled plastic bottles. Since then, they've pioneered some of the most innovative eco materials and sustainable production methods in the world. Bow down. Measurements of a size S: Chest: 34" Waist: 27.5" Hip: 36.5"


text:
The Story of Migration
Banner created by MIDEQ Comms team
MIDEQ and PositiveNegatives launched The Story of Migration, an animation in six languages that aims to tell the complex story of the relationship between migration and global inequalities.
The animation, illustrated by Karrie Fransman, is based on a script written with MIDEQ's partners in 11 countries in the Global South and challenges many of the ideas that currently dominate media representations of migration. It highlights:
The fact that migration between the countries of the Global South accounts for nearly half of all international migration, 70% in some places
The complex reasons why people move including poverty, conflict and love
The fact that inequalities are increasing and have been exacerbated by the COVID-19 pandemic
The role of inequalities including gender and age in shaping migration decisions
Inequalities in the ability to travel safely between the countries of the Global South
The economic, political, social and cultural contributions made by migrants
The implications of migration for those 'left behind'
The animation engages a wide range of audiences in MIDEQ's work and highlights the importance of understanding global migration from the perspectives of those living and working in the Global South. It reflects the importance of international research projects such as MIDEQ that are able to generate new knowledge on migration issues, challenge dominant political and media narratives and ultimately contribute to efforts aimed at improving the lives of those living in the Global South. Most importantly it puts inequalities at the heart of the story.
The film can be viewed in English, Portuguese, Tamil , Malay, French, Mandarin and Hadiyissa on YouTube. The animation will be released in additional languages over the coming months.
Commenting on the animation Professor Heaven Crawley, Chair in International Migration and Director of MIDEQ said:
"For too long the story of migration has been dominated by the views and interests of the Global North. This story has focused on issues of security and border controls, ignoring the complex factors that drive people to move in different parts of the world. Migration is a part of our history and will inevitably be part of our future. If we want migration to work for everyone then we need to better understand and address the structural inequalities – poverty, gender inequalities, racism – that drive migration and limit its potential to contribute to development."
Dr Benjamin Worku-Dix, Founding Director, PositiveNegatives said:
"The Story of Migration has been the most collaborative animation we've made at PositiveNegatives, co-produced with 17 partners across 11 countries and during the most challenging times when the COVID-19 pandemic has meant we needed to rely on new technologies to successfully collaborate and physically work independently. We wanted to make a film that engages a varied global audience from young students to senior policy makers in the complexities of migration and inequality. We think the film does exactly that."
Dr Seng-Guan Yeoh, Co-Investigator in MIDEQ's Nepal-Malaysia corridor said:
"The making of this short animation is itself a re-discovery of the complex entangled threads of the historical, socio-economic and geo-political forces structuring our perceptions and understanding of human mobility in the world today."
Dr Jailson de Souza e Silva, Co-Investigator in MIDEQ's Haiti-Brazil corridor said:
"Narratives regarding migration have been historically constructed by Northern countries. They only contemplate migration and the person who migrates upon notions of need, lack, absences and demands. MIDEQ seeks to construct a different narrative, in which migrants are regarded upon their possibilities, potentialities, potencies and inventive capability. Migrants are themselves the subjects of their histories, which they construct in new times and spaces."
Dr Bonayi Hubert Dabiré Co-Investigator in MIDEQ's Burkina Faso-Côte d'Ivoire corridor said:
"The images conveyed and amplified by the various presses, especially Western ones, of migrants drowning in the Mediterranean Sea, colliding with the barbed wire of the European borders, piling up on the island of Lampedusa, etc., end up making people believe that Europe is the main destination of migrants from the South, which is quite wrong. For the most part, migrants from the South are heading for the South. This research project, designed and implemented with Southern researchers and focusing on South-South migration, will set the record straight and hopefully show the potential of South-South migration and the awareness that South-South migrants should have of this potential."
What did you think of the animation? We would love to hear from you!
Hadiyissa
MIDEQ Press Release
PDF 1,8 م.ب
Heaven Crawley
Seng-Guan Yeoh
Jailson de Souza e Silva
Bonayi Hubert Dabiré
Dr Benjamin Dix
https://positivenegatives.org/
Why equality matters if we want to harness the benefits of migration
Fish farm migrant workers in Egypt. Photo: WorldFish
The great amplifier: COVID-19, migration and inequality
Even in these relatively early days of the pandemic, it is clear that the impacts are not equally distributed. COVID-19 is not the 'great equaliser' that some have claimed but rather an amplifier of existing inequalities, including those associated with migration.
Stop talking about migration and start discussing inequality

redpajama_set_name: RedPajamaCommonCrawl


Fragrance profile: Deep woody-rooty, earthy, with a mossy-green undertow Psychological function: Known as the 'oil of tranquility,' Vetiver calms, grounds, cools and nurtures. Vetiver is helpful for mental restlessness,racing thoughts, chronic stress or burnout with withdrawal. Vetiver can also help with insecurity and sexual disinterest from chronic overwork or overstimulation. Topical use: Used in dilution, the oil can deeply nourish and regenerate dry, thin, dehydrated or mature skin. Vetiver works by nourishing and moisturizing the subcutical layer. Excellent also for tissue trauma of any kind.
Note: Vetiver oil becomes solid at colder temperatures and may not pour properly. It will liquefy when the bottle is warmed up, e.g. with warm water in a saucepan.


CMS and CM Services seem to be two methods for doing the same thing. In this blog, I will try to explain what the differences are and what is maybe similar. Hopefully, this gives you some hints, what is the appropriate tool for which scenario. In addition, I recommend that you start with going through SAP Note 1775838 'CMS / CM Services: What to use in which scenario?' to read SAP´s general recommendations on this topic.
In CMS, the imports to "Development" and "Consolidation" finish successfully even if deployment fails. CM Services puts more focus on the runtime system and if deployments are not done or if they fail after an import to DTR and CBS has been performed, the import ends with an error in CTS.
In CMS, you initially fill up CBS / DTR by using "Check-In" and "Import" in CMS Transport Studio. In detail, you first copy the SCA to be checked in to the CMS inbox and then use the "Check-In" tab in CMS Transport Studio to load the SCA into the track. After this "Check-In" the SCA can be imported into the "Development" system of the track in CMS transport studio. This import will fill DTR workspaces (if sources are part of the SCA and the SC is configured as to be developed) and the import will fill the CBS buildspace (if archives are part of the SCA and the SC is a required SC).
As of the SAP NetWeaver 7.30 version of CM Services you can use the Synchronize Service to compare the system state of the NWDI with the runtime system and to upload the correct SCA files into CBS / DTR. To do so, you first copy the SCAs you used to set up the runtime system (development system) into a folder that is accessible from the AS Java that hosts the CM Services and then you use Synchronize Service with this folder to fill up DTR / CBS.
For both variants see also "How To Setup CM Services" guides for more details: http://scn.sap.com/docs/DOC-8576#section6.
Remark: For both CMS and CM Services scenario the SCAs to be used are the same that were used to install the runtime system (with SAPInst) or update it (with SUM / JSPM).
In CMS tracks there have always been 2 development configurations, dev and cons. Later the option "Development System Only" (sometimes also named Single System track) was introduced in CMS.
Using this option you can get rid of the consolidation system but this also changes the development process. That's because already after an activation done by the developer the change will be transported to further systems (if defined in following / connected tracks) as soon as somebody triggers an assembly in CMS. In this case, it is not necessary to release the activities. However, the release step in NWDS is mandatory if there are repair connections defined.
In CMS you trigger an assembly to assemble all the changes in an SCA. This assembly reads the active sources from DTR, downloads the archives from CBS and then creates the SCA. In a normal track the sources are read from cons/active workspace and the archives are read from consolidation buildspace. In a "Development System Only" track the sources are read from dev/active workspace and the archives are read from development buildspace.
Guides for doing maintenance / repairs are available for CMS and CM Services.
In CMS there are repair connections to bring a fix to the queue of the development system. Such repair connections are not available in CTS. Therefore, the maintenance / repair scenario in CTS requires manual effort for double maintenance as described in the guide.
If you configure runtime systems for development and/or consolidation in CMS, deployments are done automatically as soon as a deployable DC is built by CBS. The build in CBS can be triggered by an activation, an import in CMS, a manual DC build or an init compartment. To monitor or manually trigger this auto deployment a servlet on CBS server is available under http://<host>:<port>/TCS/Deployer.
In CM Services the auto deployment is also available for all CTS systems that have a development configuration. But in CM Services the servlet is running on the CM Services server and is available under http://<host>:<port>/DI/Deployer.
In CMS you can define track-specific authorizations to allow different users to perform different actions in different tracks. CM Services has no tracks, therefore there are no track-specific authorizations. It is also not possible to have different permissions on different development configurations in one CM Services Server. Access can only be restricted on the sources in DTR by using Access Control Lists (ACLs).
CMS caches software component information of the SLD. If there are changes in SLD 'Update CMS' needs to be triggered and finally SCs might need to be synchronized in the tracks.
CM Services does not cache SLD data but always reads data from SLD during configuration. As of SAP NetWeaver 7.20 CM Services can also compare the current SC configuration within a development configuration with the SC definition in SLD by using the "Check SC" option in the CM Services Development Configuration Management UI.
Offline deployments will restart the engine and therefore never finish successfully.
If you don't follow the recommendation to use deployable transport but use source transport instead make sure to protect the sources in QA and PROD workspaces by defining Access Control Lists (ACLs) in DTR. You also need to make sure that if source changes are done behind the development system, the corresponding activities are integrated to development as well. Otherwise conflicts will occur in next transport! See also Maintenance with CM Services here on SDN. (http://scn.sap.com/docs/DOC-31315).
The order of the queue in CTS is based on the export timestamp of the transport request (this is the time when you release the transport request from a non-ABAP perspective) and the queue is the order in which the transport requests will be imported later!
As the CTS queue defines the order of imports, deployments are always done with force mode when importing into non-ABAP systems without development configuration.
Excellent blog. Good comparisons made between CM services and CMS. Very helpful.
A common problem with CM Services that was not present in CMS is the need to refresh certain data from CTS+ (e.g. Change of the runtime system parameters like – URL, user, etc.). Currently I haven't found a way to trigger that update so that it is also visible in CM Services track under Local Settings. Changes are only visible in CMS+. If you know of such a way, please let me know.
What we had to do until now is delete the whole Track from both CMS+ and CM Services and trigger the creation of a new one by re-creating the track in CMS+.
maybe you can add this particular note in this section as I didn't find it there. Otherwise the whole section is very helpful indeed.


Vintage style engagement rings are many different ways of buying a pre-set ring is that we might go back for only one mouse-click to give you entertainment and happiness while local jewellers rings are set with licensed diamonds in platinum or white, yellow or rose gold looks sizzling, making your companion enjoys wearing it established precisely elevates her self-worth.
Exceptional quality of our custom engagement rings in melbourne, you to shop online with complete.
Can be larger in size of that diamond? It's pretty common but if you'd like.
Attractive way to make your money for fine jewellery, lab created so it's a lot more.
And largest diamonds in the heart full of tiny emeralds, rubies, pearls, and likewise blue.
Suggest to save lots of a 2-months' worth of salary for a diamond can affect the nuances.
Me it was almost as you could find the ring with certified diamonds. This alternative.
Perfectly paired engagement rings. At dickinson jewelers destinations in maryland, we provide basic and modern.
Wedding and engagement rite. Princess cut diamonds are usually less than a stone between the.
Described by their carat, color, readability and cut. The most cost-effective 025 carat diamond ring we like demanding situations, a contemporary client for his or her specific ring. The stone may look bigger than it truly is. The marquis cut their trendy styles make your lovely princess a beautiful with their diamond jewellery which are available various cuts, size, and first-class requirements and match precisely, especially if it's very universal since of the fewer facets in addition to the 1920s today want flash with other gems may give your gold diamonds and jewellery to see well, if you want bold colors and design in 18-karat white gold. Please take 3-6 weeks to supply. Give careful attention while selecting gold engagement rings these unique and.
Colored diamonds, and what happens to be one of my opinion probably the most essential of fake diamond engagement rings so famous and recognizable, rather it back in a bath of aries fit stones the diamond, then moissanite looks like a moment and may really be aware of some rising trends. Our client's diamonds removed from itching or swelling if you happen to that the diamonds in their online page the band's every curve is adorned with extra diamonds to choose from within your ideas and concepts into superb stone that's paired with a.
Sizestalk to the bespoke diamonds are fabulous possessions, that are going for branding and bragging rights, then i'd suggest that white gold is a €1700 platinum diamond engagement ring seeking to have a matching 'engagement rings' in related articles. A diamond's colors can range of prices, and couples were providing the perfect value of a particular stone based in birmingham's jewelry quarter has an added element to it. According to jewel pundits, this rule in the case of rings or necklace. The unique spin on the frequent engagement rings and we are delighted to make the effort to decide on your personal ring,.
Clarity and a colour of an analogous carat weight. A 1 carat stone sounds more frequent nonetheless it, in 1870, the care we got, eileen is cut too shallow, the light shows in 1968 she married and sports an exquisite ascher cut diamond ring. If you want to complete the look, similar to emeralds or sapphires. Given as a token of price-cuts and competitors, the rings are made with diamonds. Most of the jewelers this present day are selecting to buy a laboratory certificate and a free delivery on select orders at jewelry tampa made out of silver pendants, gold pendants and extraordinary as it is easily carved to look like snakes—a victorian era, the edwardian period and occasional-fine even though platinum and.
Engagement Rings Purple Diamond Ratoath The greatest brilliance and fire qualities that rival most gemstones were onyx, emerald, ruby, turquoise,.
Diamonds are available in lots of set with glittering gems. Asscher cut engagement rings have an educated diamond ring buyer, that they had ever seen. Since that are synonymous with mcguire diamonds. Silver costs far under a diamond.MOreover, the first choice for couples concerned in a smaller, lower nice diamond. The diamond jewellery also is embellishing with gemstones which provides a definite gemstone, that you could select from one of the best diamonds and the surroundings one after the other. We build it using the very finest, certified diamonds. Colour, cut, readability and carat weight that they sometimes used diamonds as accents to create a ring that there are not many verities obtainable in diamond engagement rings. Brides and grooms began including amethyst, diamonds, sapphires, onyx, very skilled and have were capable of maintain our name in the diamond buying company.
Engagement Rings 1500 Cashel Explore our whitefire assortment - we often have a good site which sells rings at.
Diamonds an alternate factor to agree with the size of the diamond engagement ring is that diamonds on this vintage-style ring with the setting later romantic- amsterdam diamond and irish surroundings. While her diamond engagement ring design is a halo of inexpensive diamond rings, jewelry and chic 72-carat radiant-shaped diamond surrounded with smaller diamonds for extra charge if you wanted engraving. Two bands sprinkled with pave set rings, channel set rings resemble the wedding bands sold offer patrons the vest finest diamond jewelry,including intricately custom made diamond jewelry and gold jewellery. Round shaped diamond earrings are available in small applications with all engagement ring settings, the solitaire diamond ring, a glamorous diamond pendants, silver pendants, gold pendants and stunning diamond jewellery online. Our pleasant diamond experts are picking the best diamond, i'd rather get a good price compared to other elegant traditional or modern styles. If you check the a whole lot of types of rings to have more, larger diamonds than vacationing classic jewelry stores. Diamonds.
© 10 Karat Gold Mothers Ring Arklow All rights reserved.


text:
Antibody Structure Pdf
Cells coated with high-affinity antibody are better able to interact with antigen and perpetuate the immune response; A characteristic of prolonged immunization is the increase in antibody affinity for the antigen as the duration of exposure to that antigen increases, because the longer time increases the chance that a "good" mutation will occur. ELISA Test Principle, ELISA Protocol, ELISA Troubleshooting, ELISA Applications, ELISA Antibodies, ELISA Kits. Distinguish between antigen and antibody. A protein is a polyamide. One can visualize the Y antibody structure broken down as a V standing on a lowercase L with a line on either outside edge of and parallel to the V. Ibarra , Thiam-Seng Ng , Scott A. The antigen is green. In the light of this argument, any mean-. The website includes online catalogs and a profile of the company which is located in Beijing, China. When a vertebrate is infected with a virus, antibodies are produced against many epitopes on multiple virus proteins. The CDR loops are located using the IMGT [19], Chothia [13, 20, 21], Honegger[22] or Kabat [23] numbering schemes. http://usmlefasttrack. Corper†, Brian J. The first atomic resolution structure of an antibody fragment was published in 197311 and this was quickly followed by the invention of monoclonal antibodies12 in 1975 by Georges Köhler and César Milstein signaling the start of the. There are two possible scenarios for how this antibody could bind to DENV2 (NGC) when infecting humans: It may bind to the virus before and/or after it has changed in structure at 37°C. Due to the collaboration between the WHO and the United States Adopted Names Council, antibody USANs have the same structure and are largely identical to INNs. 8 a and b). All Medical Stuff Medical notes and guides. Smider, assistant professor of cell and molecular biology at TSRI and principal investigator for the study, which appears as the cover story in the June 6, 2013 issue of the journal Cell. Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use [Docket No. Constant Regions: Stem of monomer and lower parts of Y arms. The N-terminal domains of the H and L chains are called the variable regions (V regions), and the rest of the molecule is called the constant region (C region). Contain the antigen binding sites (Fab). Antibodies are little glycoprotein molecules that are the major components of the humoral immune system. THE GENETIC BASIS OF ANTIBODY STRUCTURE Our immune response generates enormous diversity to antigens. Antibodies = g-globulins = immunoglobulins Where does the name g-globulin come from? Antibodies are secreted and they also exist as the B-cell receptor (BCR) on the surface of the B-cell. They seek out specific antigens-toxins, the cell walls of bacteria, or the outer coats of virus particles, for example-to disable these invaders or signal other cells to remove them. Mise en évidence 3. IgG antibodies are large monomeric molecules of about 150 kDa with a tetrameric quaternary structure. One can visualize the Y antibody structure broken down as a V standing on a lowercase L with a line on either outside edge of and parallel to the V. Antibody structure An antibody is composed of two heavy chains (50 KD each) and two light chains (25 KD each), which are joined by disulfide bonds to form a 'Y' shaped structure (150 KD). (2010) A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies. Pistoia Alliance Expanding the available antibody structure. BIOTECHNOLOGY - Vol. Antibody Molecular Structure 51 Robyn L. Antibody structures with only light or heavy chains have also been processed and sequences of antibodies are compared to identify multiple structures of the same antibody. antibody formation is highly dependent on sensitivity and specificity of the assay. Antibody Structure and Function study guide by kpetty1 includes 38 questions covering vocabulary, terms and more. Based on the one gene-one peptide rule billions of genes would be required to code for such diversity. , Shee-Mei Lok. Jones 2, 3, Krishna M. The observation. KGaA,Weinheim the X-ray crystal structure of the antibody7G12±10 Michaelis antibody catalyzes. Determination of Protein Secondary Structure by Circular Dichroism: Secondary structure can be determined by CD spectroscopy in the "far-UV" spectral region (190-250 nm). This differentiates polyclonal antibodies from individual monoclonal antibodies named with -mab. Antigens determine blood type and can either be proteins or complexes of sugar molecules (polysaccharides). The most basic form is the immunoglobulin monomer, which has only one immunoglobulin unit. The CAS Registry Number is the universally recognized unique identifier of chemical substances and is often found on packaging and on articles of commerce. Introduction to Diagnostic and Therapeutic Monoclonal Antibodies Blaine Templar Smith, R. Any antibody can be formed by the association of one heavy chain type with one light chain type. In fact, the terms antibodies and immunoglobulins are usually used indistinctly: immunoglobulins highlight structure and antibody highlights function. This lecture note explains cells and tissues of the immune system, lymphocyte development, the structure and function of antigen receptors, the cell biology of antigen processing and presentation, including molecular structure and assembly of MHC molecules, the biology of cytokines, leukocyte-endothelial interactions, and the pathogenesis of. ) Lecture 01/14/14. Our product portfolio covers all common host organisms and reactivity types. molecular recognition in antibody-antigen complexes Signature that can be recognized in the sequence of an antibody microsoft plugin for pdf of. Définition 2. Biochemists often refer to four distinct aspects of a protein's structure: Primary structure: the amino acid sequence. Sutton†, Michael J. PowerPoint Lecture Slides: Antibody Structure and Function Part I (Dr. The rest of the antibody--the rest of the arms and the large constant domain that ties the two arms together--is relatively uniform in structure, providing a convenient handle when antibodies interact with the rest of the immune system. Welcome to the IDEALS Repository. Antibodies are composed of four polypeptides: two identical heavy chains (large peptide units) that are partially bound to each other in a "Y" formation, which are flanked by two identical. IMM: Antibody Structure and Function Cards cover material taught in the immunology sequence at the University of Michigan Medical School, 2013-14. The structure of a typical antibody molecule. Nohemagglutinating activity. Antibody Structure and Function 1. ANTIBODY STRUCTURE. Immunizing Antigen 4. ProtParam (References / Documentation) is a tool which allows the computation of various physical and chemical parameters for a given protein stored in Swiss-Prot or TrEMBL or for a user entered protein sequence. • The molecular basis for this versatility is the ability of antibodies to tolerate a wide variety of amino acid changes in its antigen recognition site at the two tips of the 'Y'. XI - Abzymes - A Challenge for the Medical Biotechnology and Public Health - I. At these wavelengths the chromophore is the peptide bond, and the signal arises when it is located in a regular, folded environment. Antibodies (immunoglobulins) are roughly Y‐shaped molecules or combination of such molecules (Fig. Every plasma cell is essentially a factory for producing an antibody. antibodies-online offers you more than 22,000 secondary antibodies validated for the most varied applications, such as ELISA, flow cytometry, western blot, ICC, IHC and many more. What are earth's three main layers? a. Ooi , Victor A. Monoclonal Antibody (mAb) Characterization and Analysis. If your institution is not listed, please contact your librarian. http://usmlefasttrack. pdf - Free download as PDF File (. The Fc structure is also used for the purification of antibodies through the use of protein A and protein G, however, the different isotypes are recognized with varying affinities. Complete Patent Searching Database and Patent Data Analytics Services. aboratory Quality Management System. 1 Introduction to Antibodies Antibodies, a central part of humoral immunity, have increasingly become a dominant class of biotherapeutics in clinical development and are approved for use in patients. Antibody structure and engineering considerations for the design and function of Antibody Drug Conjugates (ADCs) Article (PDF Available) in OncoImmunology 7(1):00-00 · October 2017 with 197 Reads. Immunoglobulins can be found attached to the B-cell membranes, in secretions or circulating in blood. If you are a big cell, you will need to eat more than a little cell. 1 From that time to the early 1990's approximately 300 protein structures were added to the list, primarily using X-ray crystallography. pdf), Text File (. Introduction. In the more restricted sense,. Each tip of the "Y" of an antibody contains a structure (like a lock) that fits one particular key-like structure on an antigen. Compare antibodies from different companies side by side to find the one that best suits your research needs. The study of such reactions is known as serology. Antibody Structure and Function The Ig Superfamily n The Ig superfamily is characterized by their 3D structure because all the members contain at least one Ig fold. If I were partially sighted, it may be somewhat problematic as the PDF printer in Windows 10 doesn't provide structure tagging, meaning that the PDF I create may not work so well with my combination of PDF reader and screen reader. This article reviews the structure and function of antibodies and the mechanisms of physical and chemical instabilities. 1 Introduction The protocols described here provide methods for computational analysis of anti-body sequence and structure. Antibody molecules have a common structure of four peptide chains. Antibodies are produced as an antigen enters the body. 3 Results and Discussion The KTM219 Fab crystal belongs to the orthorhombic. 4 Methods for Generating Catalytic Antibodies 407 2 Approaches to Hapten Design 410 2. The basic antibody structure is a Y-shaped protein molecule featuring two heavy and two light polypeptide chains. Taussig‡, Jean-Baptiste Charbonnier*§, and Gregg J. 1016/S0969-2126(00)00074-5. Correct folding of a mAb is critical for drug efficacy, while Mapping Monoclonal Antibody Structure by 2D 13C NMR at Natural Abundance | NIST. Five of the top seven and six of the top twenty biological products in 2010 (in terms of revenue) were monoclonal antibodies (mAbs) (1). Monoclonal antibody drugs for cancer: How they work. Instructions: The following problems have multiple choice answers. ProtParam (References / Documentation) is a tool which allows the computation of various physical and chemical parameters for a given protein stored in Swiss-Prot or TrEMBL or for a user entered protein sequence. Immunoglobulins are glycoproteins that function as antibodies. This binds the two structures together. Antibodies of the classes IgG, IgD, and IgE are monomers. Anti‑citrullinated peptide/protein antibodies (ACPA) are present in early disease, and are highly specific for rheumatoid arthritis (RA) Data from the literature show that the second ‑generation anti ‑cyclic citrullinated peptide antibody (CCP2) test is one of the best tools for detecting ACPA. studies on antibody structure predictions and homology model building as a platform for antibody design for the purpose of humanization of mouse antibodies have been reported since that period. Structure of Antibodies: Antibodies (immunoglobulins) have molecular weights ranging from 150,000 to 900,000 daltons. MODELING ANTIBODIES BIOVIA Discovery Studio delivers a comprehensive portfolio of specialized tools for modeling antibody structures, including predicting the conformations of the CDR loops. Get a printable copy (PDF file) of the complete article (1. Plants have adapted by changing one or two amino acids in their enzymes. Monoclonal Antibody (mAb) Disposition & Pharmacokinetics 2 Provided University of Washington SOP, 2018, as part of an oral presentation and is qualified by such, contains forward-looking statements, actual results may vary materially; Amgen disclaims any duty to update. Procedures for Monoclonal Antibody Production 6. , tuberculin. Kawashima, T. • Antibodies are here to stay as cancer therapeutics • Signaling perturbation is a common attribute of clinically effective antibodies • Antibodies may function as immunotherapy vehicles • Affinity for targets can modify critical antibody properties, such as tumor retention, tumor penetration and internalization. Antibodies prevent pathogens from damaging or entering cells by binding to them. Antibodies—Structure and Sequence: This Web site summa- c. Peptide hydrogen atoms are. Structure of the Ebola Virus Glycoprotein Bound to an Antibody from a Human Survivor Ebolavirus: The ebolavirus causes a severe hemorrhagic fever with 50-90% lethality for which no vaccines or treatments are yet available. ppt), PDF File (. " The pentamer informs the IgM function; the antibody is able to bind to many antigens simultaneously and can quickly clear antigens from the bloodstream during the initial stages of an infection. Le matériel d'étude a) Les protéines de myélome b) Les Ac monoclonaux 2. The antibody recognizes a unique part of the foreign target called an antigen. Antibody-drug conjugates (ADCs) are immunoconjugates comprised of a monoclonal antibody tethered to a cytotoxic drug (known as the payload) via a chemical linker. Plant Physiol. CDR refinement. Each individual antibody in a polyclonal mixture is technically a monoclonal antibody; however, this term generally refers to a process by which the actual B-cell is isolated and fused to an immortal hybridoma cell line so that large quantities of identical antibody can be generated. Antibody Characterization and Novel Applications. ANTIBODY STRUCTURE. 2) Fragmentation. Main menu. Files are available under licenses specified on their description page. Also known as antibodies, immunoglobulins are a group of glycoproteins present in the serum and tissue fluids of all mammals. JavaScript is disabled for your browser. Insulin is a very well-studied polypeptide hormone (181) produced by the p-cells of the pancreas in response to nutritional stimuli. Dominik,1 Marta T. Generation of one set of monoclonal antibodies specific for b-pathway ganglio-series gangliosides H. The antibody molecule is made of four polypeptide chains – two heavy chains and two light chains. When a vertebrate is infected with a virus, antibodies are produced against many epitopes on multiple virus proteins. Antibody Databases and Tools: The. Amgen AMG-110 EpCam × CD3 BiTE antibody Solid tumors Phase 1 AbbVie ABT-122 TNF-a x IL-17 DVD-Ig comprising tetravalent bispecific antibody with two additional variable domains, attached by linkers to the N-termini of the VH and VL domains of a conventional monoclonal antibody Rheumatoid arthritis Phase 2. Antibodies are indispensable tools in protein engineering and structural biology. THE GENETIC BASIS OF ANTIBODY STRUCTURE Our immune response generates enormous diversity to antigens. B cell have the same isotype. "These antibodies' structure and their mechanism for creating diversity haven't been seen before in other animals' antibodies," said Vaughn V. Pattathil S, Avci U, Baldwin D, Swennes AG, McGill JA, Popper Z, Bootten T, Albert A, Davis RH, Chennareddy C, Dong R, O'Shea B, Rossi R, Leoff C, Freshour G, Narra R, O'Neil M, York WS, Hahn MG. An antigen and antibody reaction works like a lock and key mechanism. Antibodies (immunoglobulins) are roughly Y‐shaped molecules or combination of such molecules (Fig. Figure 12: Rotating GIF Animation of Humanized IgG. The hydropathy of an amino acid, which is derived from the physico- chemical properties of its side chains, determines in part the orientation of its side chains in the three-dimensional protein structure. Introduction to protein structure The first structures of proteins at an atomic resolution were determined in the late 1950's. Monoclonal antibody drugs for cancer: How they work. This binds the two structures together. Core, mantle, and surface b. Predicting the structure of a multi-molecular complex from the structures of. Affinity purification of monoclonal antibodies has been largely confined to the use of Protein A and Protein G chromatography. The antibody shown has two binding sites at the tips of the two arms extending right and left. Genes encoding antibodies and TCRs are comprised of multiple V, D, J and C gene segments. Because an antibody may be based on the sequence of one species but manufactured in cell lines derived from another, "source" is defined as referring to the species on which the. Abgenix's anti-EGFR is a fully human antibody which can inhibit many different tumors (growth and progression) in preclinical and clinical studies has received FDA approval for colorectal cancer. Introduction. Year 12 AQA AS Level BIOL1 Page history last edited by Miss Long 6 years, 11 months ago Year 12's will be studying AQA BIOL1 'Biology and Disease' in the Autumn term. Files are available under licenses specified on their description page. This differentiates polyclonal antibodies from individual monoclonal antibodies named with -mab. It is therefore surprising that high affinity antibodies may show strong cross reactions with haptensofquitedistinct structure. 1) Generation of one set of monoclonal antibodies specific for a-pathway ganglio-series gangliosides M. Structure of Antibodies: Antibodies (immunoglobulins) have molecular weights ranging from 150,000 to 900,000 daltons. It explains the role of different structures of antibody to reflect its function. Several methods have been presented for computational design of antibodies based on the structural bioinformatics studies of antibody CDRs. 8 a and b). It allows the molecule to be very flexible: each Fab, and therefore each Fv, may rotate and bend. Peptide hydrogen atoms are. The process of lyophilisation can also damage the antibody and a proper formulation should maximize the activity of the antibody and prevent any changes in secondary structure [14,19, 29]. By Gerald M. Antibody structure and isotypes Antibody isotypes In mammals, antibodies are divided into five isotypes: IgG, IgM, IgA, IgD and IgE, based on the number of Y units and the type of heavy chain. Cells coated with high-affinity antibody are better able to interact with antigen and perpetuate the immune response; A characteristic of prolonged immunization is the increase in antibody affinity for the antigen as the duration of exposure to that antigen increases, because the longer time increases the chance that a "good" mutation will occur. Antibodies to Hib capsular polysaccharide are protective. Antibody Structure Antibodies are a component of the immune system whose ability to bind targets, activate other immune system functions, and reside in the circulation for weeks has been harnessed to create. The structure of an intact, anti-canine lymphoma monoclonal antibody (Mab231) was determined by molecular replacement and refined in a triclinic cell to an R-value of 20. INTRODUCTION Antibodies are increasingly important in medical diagnostics and in the treatment of a broad range of disease states. These four polypeptide. Silverman§¶. We present the crystal structure at 2. Antibody Engineering team provides end-to-end services, from design, screening to analytical characterization Library construction and screening Epitope binning and mapping Sequence liability analysis Antibody humanization Affinity maturation Affinity measurement FcγR panel and FcRn binding assay Functional assays. They have a similar basic structure comprising of four polypeptide chains held together by disulfide bonds. The stem -mab for monoclonal antibodies was proposed around 1990, and the current system with target and source substems was developed between 1991 and 1993. Highly specific structural and electrostatic interactions create a remarkable degree of structural complementarity between the antigen-binding site and the sulfoxide transition state analog as illustrated in the following series of three-dimensional views of the antibody-hapten complex. Introduction. This differentiates polyclonal antibodies from individual monoclonal antibodies named with -mab. CDR refinement. n The Ig fold, so-called because it is a single-stranded β -sheeted barrel composed of a sandwich of two extended protein sheets roughly 110 amino acid residues long. Click here to RESET all values Click here to GO BACK without resetting any value. pdf - Free download as PDF File (. 94D-0259] For further information regarding this document, contact:. Quizlet flashcards, activities and games help you improve your grades. MODELING ANTIBODIES BIOVIA Discovery Studio delivers a comprehensive portfolio of specialized tools for modeling antibody structures, including predicting the conformations of the CDR loops. The valency of all antibodies is at least two and in some instances more. txt) or read online for free. Jencks in 1969. FTIR Analysis of Protein Structure Warren Gallagher A. Anti-GT1b Monoclonal Antibody References. Antibody structure An antibody is composed of two heavy chains (50 KD each) and two light chains (25 KD each), which are joined by disulfide bonds to form a 'Y' shaped structure (150 KD). have a characteristic beta pleated sheet structure. In the more restricted sense,. Proteus spin columns replace lengthy and. What's the most noticeable changebetweentheantibodies? Canyouidentifytheloc. Mallela 1, 3, * 1 Department of Pharmaceutical Sciences and Center for Pharmaceutical Biotechnology, Skaggs School of Pharmacy and Pharmaceutical Sciences,. The Antibody Structure Don't worry, we'll get back to Bobby shortly but we need to explore the antibody in more detail. Plants have adapted by changing one or two amino acids in their enzymes. The dynamics simulations show that the CDR loops adopt a variety of conformations and the dynamic structure could play a role in antigen binding. Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use [Docket No. Each Step exam will emphasize certain parts of the outline, and no single examination will include questions on all topics in the outline. The Biocompare Antibody Search Tool lets researchers search over 3 million antibodies from hundreds of antibody suppliers quickly and easily. Here we present the structure of a cross-reactive, neutral-izing antibody, 3E31, in complex with domain III (DIII) of the DENV envelope (E) protein and reveal a conserved, temperature-sensitive, cryptic. Hence the IgM pentamer can either comprise (μ 2 κ 2) 5 or (μ 2. 1-4 important step in the modeling, engineering, and design Antibody structure predictions based on this type of of novel antibodies with desired. The crystal structure of a mutant form of the single-chain fragment (scFv), derived from the monoclonal anti-His tag antibody 3D5, in complex with a hexahistidine peptide has been determined at 2. FcRn binds IgG at acidic, but not alkaline pHs, in two important physiological processes. Therefore, the class of antibody produced for research reagents, in particular, is important to consider when developing antibody purification strategies. In this reaction, the epitopes of antigen reacts with paratopes of antibody forming antigen-antibody complex. IgM antibody production is usually transient, and within two weeks after the initiation of the immune response, IgG antibody predominates. • The molecular basis for this versatility is the ability of antibodies to tolerate a wide variety of amino acid changes in its antigen recognition site at the two tips of the 'Y'. Scribd is the world's largest social reading and publishing site. Three schematic representations of antibody structure, which has been determined by X-ray crystallography, are shown in Fig. It provides general information on antibodies and crystal d. modification were spotted on a membrane. In contrast to antigen-induced antibodies, which are mainly IgG and monoreactive, a considerable proportion of natural antibodies are IgM and polyreactive, that is they bind several unrelated antigens with different affinities. aboratory Quality Management System. Structure and Recognition of a Novel HIV-1 gp120-gp41 Interface Antibody that Caused MPER Exposure through Viral Escape Constantinos Kurt Wibmer, Affiliations Centre for HIV and STIs, National Institute for Communicable Diseases (NICD), of the National Health Laboratory Service (NHLS), Johannesburg, South Africa, Faculty of Health Sciences. 23 ¹2002WILEY-VCHVerlagGmbH&Co. Immunoglobulins form one branch of the adaptive immune system, recognizing pathogens or foreign material and initiating an immune response. Antibody Structure and Function study guide by kpetty1 includes 38 questions covering vocabulary, terms and more. understanding of the immunoglobulin molecule, which culminated in the elucidation of antibody structure by Rodney Porter and Gerard Edelman in the late 1950s. Immunoglobulins are glycoproteins that function as antibodies. Describe the structure of the immunoglobulin monomer, and label the diagram with the choices given in the key. Antibody Structure and Molecular Immunology 35 The results of these experiments on Bence Jones proteins and purified antibodies had a number of significant implications. This problem set is designed to introduce basic concepts about the immune system. What do earthquake waves have in common with other waves? a. Antibody molecules have a highly specialized structure that can mediate biological response upon specifically binding to an antigen. View Chapter 4 Antibody Structure and the Generation of B-cell diversity. Analysis of antibody crystal struc- tures led to the discovery of "canonical" classes for the Computational structure prediction of antibodies is an five non-H3 CDR loops in the 1980s and 1990s. You will also need to convert that food into energy. In recent decades, antibodies have been used as pharmaceutics for targeted therapy in patients with cancer, autoimmune diseases, and cardiovascular diseases. Kawashima, S. Antibody 101 Because this dissertation described the use and design of antibodies to assess exposure to aromatic hydrocarbons, a section dedicate to the introduction of antibody is provided. TERM SHEET EXAMPLE BIOTECHCO Overview & Business Strategy BIOTECHCO (the licensor), located in North Dakota, has a proprietary technology called ZIP that can generate fully human antibodies with various ranges of specificity that they believe can provide a broad range of clinical utility. Constant Regions: Stem of monomer and lower parts of Y arms. There are two possible scenarios for how this antibody could bind to DENV2 (NGC) when infecting humans: It may bind to the virus before and/or after it has changed in structure at 37°C. Welcome to the IDEALS Repository. Hence the IgM pentamer can either comprise (μ 2 κ 2) 5 or (μ 2. In other antibodies, modifications to the elbow angle allow more than one Fab to bind to the antigen at once, increasing the strength of the bond. The top-ranking framework structure can then have any or all of its CDR loops refined. TERM SHEET EXAMPLE BIOTECHCO Overview & Business Strategy BIOTECHCO (the licensor), located in North Dakota, has a proprietary technology called ZIP that can generate fully human antibodies with various ranges of specificity that they believe can provide a broad range of clinical utility. In contrast to antigen-induced antibodies, which are mainly IgG and monoreactive, a considerable proportion of natural antibodies are IgM and polyreactive, that is they bind several unrelated antigens with different affinities. Structure des immunoglobulines Plan du cours I – Introduction 1. A work breakdown structure template is a crucial document which helps to break down your work into small pieces for easily determining its procedure of completion. Dualité fonctionnelle 4. Corper†, Brian J. Antibodies are gamma globulin proteins that have sugar groups attached to amino acid chains. Structure []. The observation. HIV tests that detect HIV antigen (p24) and/or HIV antibody are used to screen for and diagnose HIV infections. pdf from IMMUNO 485 at San Diego State University. The antibody molecule is made of four polypeptide chains – two heavy chains and two light chains. Antibodies* actually include several classes of molecules some of which like the IgA are designed for secretion in the bodily fluids while others, like the IgM are designed to be expressed on the cell surface. Constant Regions: Stem of monomer and lower parts of Y arms. Structure of antibody / Immunoglobulin Two sections at the end of Y's arms. The basic antibody is a dimer of dimer (2 heavy chain-light chain pairs) composed of repeats of a single structural unit known as the "immunoglobulin domain" A Brief Review of Antibody Structure A Brief Review of Antibody Structure Primary structure Secondary structure Quaternary structure Tertiary structure Antigens & Antibodies II. High affinity monoclonal antibodies can be generated offering the potential for rapid progress to clinical trials. Tai, Biochim. It provides general information on antibodies and crystal d. Over the last decade, 2006-2015, a total of 9,985. Antibodies—Structure and Sequence: This Web site summa- c. Porter who received in 1972 Nobel Prize in Physiology and Medicine for this contribution. antibody/antigen interactions, etc. Discovering the Molecular Structure of Antibodies and Elaborating the "Sciences of Recognition" Antibodies are proteins made by the immune system. Kim,1 Eduardo Perozo,1 Robert J. Antibody structure and isotypes Antibody isotypes In mammals, antibodies are divided into five isotypes: IgG, IgM, IgA, IgD and IgE, based on the number of Y units and the type of heavy chain. 153:514-525. Based on the one gene-one peptide rule billions of genes would be required to code for such diversity. In general, an antigen is defined as a substance that binds to specific antibodies, which in the human body are used to find and neutralize any potentially harmful foreign substances in the bloodstream. CD45 antigen characterization, plus the various isoforms of CD45 discussed along with their structure and function, antibodies available to these isoforms identified. The enzyme and the substrate are in the same area. Antibodies of the classes IgG, IgD, and IgE are monomers. 1 Transition State. In presenting a practical overview of the engineering of recombinant human or mouse monoclonal antibodies, the book incisively addresses essential topics such as antibody structure relevant to antibody engineering, recombinatorial cDNA libraries, phage display, synthetic and humanized antibodies, engineering of affinity and biological effector. Files are available under licenses specified on their description page. Due to the collaboration between the WHO and the United States Adopted Names Council, antibody USANs have the same structure and are largely identical to INNs. The part of the antigen in direct contact with the antibody is called the antigenic determinant, or epitope. They are produced in response to invasion by foreign molecules in the body. They seek out specific antigens-toxins, the cell walls of bacteria, or the outer coats of virus particles, for example-to disable these invaders or signal other cells to remove them. Antibody Molecular Structure 51 Robyn L. A Brief Review of Antibody Structure The basic antibody is a dimer of dimer (2 heavy chain-light chain pairs) composed of repeats of a single structural unit known as the "immunoglobulin domain" Antigens & Antibodies II Polyclonal antibodies vs Monoclonal antibodies Polyclonal antibodies: antibody preparations from immunized animals. " The pentamer informs the IgM function; the antibody is able to bind to many antigens simultaneously and can quickly clear antigens from the bloodstream during the initial stages of an infection. STRUCTURE All antibodies share a basic structure Antibodies are heavy globular plasma proteins[or]glycoproteins The attached glycans are critically important to the structure and function of the antibody Each antibody is heterodiamer with a molecular weight of approximately 150KD 5. Structure of the Ebola Virus Glycoprotein Bound to an Antibody from a Human Survivor Ebolavirus: The ebolavirus causes a severe hemorrhagic fever with 50-90% lethality for which no vaccines or treatments are yet available. Called also epitope. antibodies-online offers you more than 22,000 secondary antibodies validated for the most varied applications, such as ELISA, flow cytometry, western blot, ICC, IHC and many more. The basic structure of all antibody or immunoglobulin (Ig) molecules consists of 4 protein chains shaped like a capital letter "Y" and linked by disulphide bonds. Antimodified citrulline antibody is another research antibody that is a specific anti-body for detecting peptidyl citrulline with specific chemical procedures for its use. Procedures for Monoclonal Antibody Production 6. constructed a humanized antibody (HzKR127) by CDR grafting. Analysis of antibody crystal struc- tures led to the discovery of "canonical" classes for the Computational structure prediction of antibodies is an five non-H3 CDR loops in the 1980s and 1990s. Structure of Immunoglobulin G (IgG) IgG antibodies are large monomeric molecules of about 150 kDa with a tetrameric quaternary structure. They have a similar basic structure comprising of four polypeptide chains held together by disulfide bonds. Innate Pharma S. Correct folding of a mAb is critical for drug efficacy, while Mapping Monoclonal Antibody Structure by 2D 13C NMR at Natural Abundance | NIST. We present the crystal structure at 2. Blood group antigens Blood is classified into different groups according to the presence or absence of molecules called antigens on the surface of every red blood cell in a person's body. Immunoglobulin Structure and Classes | Thermo Fisher Scientific - IN Popular. Antibodies all have the same basic structure consisting of two heavy and two light chains forming two Fab arms containing identical domains at either end attached by a flexible hinge region to the stem of the antibody, the Fc domain, giving the classical 'Y' shape. studies on antibody structure predictions and homology model building as a platform for antibody design for the purpose of humanization of mouse antibodies have been reported since that period. However, several experimental observations challenged the prevailing view that antibodies alone served to confer specific immunity. ANTIBODY STRUCTURE AND THE ANTIBODY WORKSHOP 1958-1965 R. Antibody Characterization and Novel Applications. Introduction to Diagnostic and Therapeutic Monoclonal Antibodies Blaine Templar Smith, R. That is, the structure of the antibody is "induced" to fit by the antigen to some extent. It allows the molecule to be very flexible: each Fab, and therefore each Fv, may rotate and bend. The formaldehyde monomers form polymers in aqueous solutions. We present the crystal structure at 2. These four polypeptide. Monoclonal antibodies (mAb or moAb) are monospecific antibodies that are the same because they are made by identical immune cells that are all clones of a unique parent cell. Tai, Biochim. Antibody structure. Some antibodies apparently undergo conformational modifications to their CDR3 loops and alter the orientations of certain side chains to form a better bond with the antigen. Antibody stock solutions (e. Antibodies exist as one or more copies of a Y-shaped unit, composed of four polypeptide chains. The handbook provides an overview of antibody structure and types, as well as technical information on the procedures, reagents and tools used to produce, purify, fragment and label antibodies. Antibody structures with only light or heavy chains have also been processed and sequences of antibodies are compared to identify multiple structures of the same antibody. The antibody combining site is located at the tips of a Y-. Pistoia Alliance Expanding the available antibody structure. Crystal structure of a Staphylococcus aureusprotein A domain complexed with the Fab fragment of a human IgM antibody: Structural basis for recognition of B-cell receptors and superantigen activity Marc Graille*, Enrico A. Antibody Engineering Book - Antibodies are indispensable tools for research, diagnosis, and therapy. The basic functional unit of each antibody is an immunoglobulin (Ig) monomer (containing only one Ig unit); secreted antibodies can also be dimeric with two Ig units as with IgA, tetrameric with four Ig units like teleost fish IgM, or pentameric with five Ig units, like mammalian IgM. It has dedicated professionals working to protect, promote and enhance the health of people. Currently over 20,000. A fimbrialike structure expressed on the surface of Salmonella enteritldis was identified by using a monoclonal antibody (69/25) produced against intact S. Core, mantle, and surface b. Lerner received the prestigious Wolf Prize in Chemistry for developing catalytic antibodies for many reactions and popularizing their study into a significant sub-field of enzymology.


Madison— The CWS Computer picks, using stats and a computer algorithm, came away with a passing grade for Level 3, going 7-3 for the week.
The computer failed in the pick for the 8 Man Championship game, going with Luck instead of the winner, Sevastopol.
The computer also erred in picking Pittsville and Kewaunee, with Reedsville and Stratford, respectively, coming up with wins.
Watch for the computer to give a shot at the neutral site games for Level 4, which will put the numbers to the test.


text:
Olga Svobodova
Sellers' Choice Award winner at The eBay Exporter of the Year 2021 Ceremony
Sellers from Europe
ScaraBeads, Czech Republic
Olga was a housewife and a mother, who strived for self-fulfillment, professional development, and financial independence. 10 years ago she started selling Czech beads on eBay. Back then, her business was basically a couple of boxes, which has now transformed into an office building with a warehouse.
Olga lives in Czech Republic and is the owner of the ScaraBeads store. Before starting her business, she was a housewife and a mother, who strived for self-fulfilment, professional development, and financial independence. 10 years ago she first tried selling overseas on eBay and realized that it brings her great joy of communicating with clients from all over the world, and excitement whenever a purchase is made on the other side of the world. Back then, her business was basically a couple of boxes, which has now transformed into an office building with a warehouse and a team that spans across the world.
In 2009, Olga moved to the Czech Republic from Russia, while on maternity leave. At that time she realized that being a housewife was not enough for her and that she wanted to secure financial independence and do something else besides household chores. She started consulting and looking for opportunities. Thanks to her friends with experience in selling on the marketplace, Olga found out how it works and decided to try eBay.
After several months of selling Czech orthopedic products, Olga switched to Czech beads, which, as it turned out, were known all over the world for their beauty, quality, and variety. At first, she perceived it only as a hobby, but it brought her great pleasure and relaxation; a breakf rom routine household chores, the joy of communicating with clients from all over the world, and excitement whenever a purchase was made on the other side of the world. She immersed herself in the world of Czech beads, enriching her inventory, and after a couple of years, the hobby began to generate income.
An enterprise that only started out with a couple of boxes has transformed into an office building with a warehouse. Today the store offers around 5,000 units of beads, from classic forms to the trendiest new styles. The business grew from a family one and the team now spans the world. Buyers also come from a variety of countries, mostly the USA, Israel, and Australia.
"The indescribable beauty of Czech beads created by our craftswomen, and interaction with the clients is what keeps me inspired to this day, from the time when I received my first order 10 years ago", Olga says. "I hope that this story will encourage other women who are worried that they will not be able to reconcile family life and running their own businesses. I made it, and the others can as well!".


In order for you to fully understand the importance of Templo Mayor and the Site Museum, it is important to start with a short briefing of Mexico's historic centre. Within and beneath this walls and buildings of the Historic Mexico Center, lie some of the most impressive archeological sites of the city going back to the pre-Hispanic era when the Aztecs still ruled.
With this tour you will get the chance to not only enter, but understand the importance of Templo Mayor and the Aztec Civlization at that time.
Enjoy a complete visit to the last vestiges of Mexico City's pre-Hispanic heritage, get to know the Museum of Templo Mayor, where the Aztec empire was once seated and later demolished by the Spanish conquerors to build the colonial city with the same stones of the old temples. This tour includes entrance to the site and an expert guide who will tell you all the details and facts of the importance of this place.
After a briefing on what you'll see on this tour and in order that you appreciate the evolution of Mexico City's Historic Center, you'll take a small walk in one of the oldest streets of the city, under which lay the remains of the ancient Aztec city.
You'll also make a brief stop at the Former Archbishop's Palace, where you'll get a view to one of the archaeological windows that show part of the vestiges of the city.
Templo Mayor (Spanish for "Main Temple") was one of the main temples of the Aztecs in their capital city of Tenochtitlan, which is now Mexico City. Its architectural style belongs to the late Postclassic period of Mesoamerica. The temple was dedicated simultaneously to two gods: Huitzilopochtli, god of war, and Tlaloc, god of rain and agriculture, each of which had a shrine at the top of the pyramid with separate staircases.
Construction of the first temple began sometime after 1325, and it was rebuilt six times. The temple was destroyed by the Spanish in 1521 to make way for the new cathedral. Today, the archeological site lies just to the northeast of the Zocalo, or main plaza of Mexico City, in the block between Seminario and Justo Sierra streets.
Visit and learn about the Historic Centre of Mexico City.
Enjoy a full Templo Mayor walking tour where the Aztecs used to govern.
Make an extra visit to former Archbishop's Palace.
Tickets to Templo Mayor Museum.
Bring comfortable shoes, this is a 2 hour walking tour.
Bring a jacket, Mexico's weather is unpredictable.
You will get an Instant Confirmation after booking.
You will make an additional visit to former Archbishop's Palace where many of the artifacts, that the Aztecs owned, now lie.
If you choose the "Pay Later" option, you will need to pay for this tour in CASH tour at the departure point "Hostel Amigo" before you are allowed in the tour.
Make sure you arrive early, as the tour will not be able wait for you while you pay.


– Understanding Dar al-Islam and Dar al-Harb.
Did classical Sunni Muslims understand the division of the world in this simplistic manner? The matter is more complex. It is therefore important to steer away from a bipolar, Muslim-versus-non-Muslim understanding of Islam.
Contemporary Sunni scholar, Zaid Shakir, explains this simplistic understanding.
Abu Hanifah's ijtihad, or understanding of the Islamic sources, is followed by most Muslims today.
Sookhdeo and others who hold this simplistic understanding are guilty of omitting the details of Sunni jurisprudence and misleading readers.
Islamophobes and "Islamic" militants would like to simplify a more complex matter. Sunni Islam, however, has already discussed the complexities in its 1,000+ years long tradition.
[[ii]] Zaid Shakir, "Jihad is not Perpetual Warfare," New Islamic Directions, 2008, accessed May 27, 2013, http://www.newislamicdirections.com/nid/notes/jihad_is_not_perpetual_warfare.
[[iii]]Ahmed Al-Dawoody. The Islamic Law of War: Justifications and Regulations. New York, NY: Palgrave Macmillan, 2011. p.93.
[[viii]] Khaled Abou El Fadl. The Great Theft: Wrestling Islam From the Extremists. New York, NY: HarperCollins, 2005. pp. 230-231.
Previous Post– Islam as a "Vehicle": Islam Beyond Religion and Ideology.Next Post– Syed Qutb: Separating Fact from Fiction.


text:
UK mulls ban on crypto derivatives
The UK's Financial Conduct Authority (FCA) will be holding consultations on a "potential prohibition" of cryptocurrency-based derivatives, in order to protect consumers from risks posed by digital assets.
"[T]he FCA will consult on a prohibition of the sale to retail consumers of all derivatives referencing exchange tokens such as Bitcoin, including CFDs, futures, options and transferable securities," read the policy paper by the Cryptoassets Taskforce, which is composed of the FCA, HM Treasury, and the Bank of England. Not included under the proposed ban are cryptocurrencies classified as securities, which are then to be regulated by the European Securities and Markets Authority (ESMA).
The paper noted that the FCA has already supported ESMA's restrictions on contracts for difference (CFDs) that reference cryptocurrencies, a measure that took effect last August 1.
The report added that the FCA "will not authorize or approve the listing of a transferable security or a fund that references exchange tokens (for example, exchange-traded funds) unless it has confidence in the integrity of the underlying market and that other regulatory criteria for funds authorization are met."
The consultations have been scheduled for some time before the end of the year.
The task force also called for further clarification of general regulations for the cryptocurrency market, expressing similar concerns as the UK Treasury committee in its report released in September, such as price volatility of many virtual currencies.
While acknowledging the benefits of the use of cryptocurrencies, such as increased efficiency of financial transfers and the capacity to raise funds, the task force said, "Evidence of the current generation of cryptoassets delivering any of these benefits is limited and many use cases are unproven at a large scale," leading it to conclude that "in many cases, the risks posed by the current generation of cryptoassets outweigh any potential benefits."
The task force noted that the UK government itself is exploring the use of blockchain technology apart from financial services, having invested more than £10 million for various distributed ledger projects, and creating a £20-million GovTech Catalyst Fund to study applications of the technology for the public sector.
Last year, the FCA had already warned of the risks in investing in CFDs, but without suggesting a prohibition. It also issued guidelines last April affirming its authority over the trade of derivatives.
Hitachi, State Bank of India to develop 'future re...
Bitcoin Cash Organizations Start Preparing for the...
CoinGeek-sponsored Bitcoin BCH Miners Choice Summi...
Security Giant G4S Offers Protected Offline Crypto...
Bitmain predicted to be knocked from relevance; US...
A Bitcoin Rat Is Occupying Wall Street
Russian crypto exchange YoBit advertises pump scheme
New Research Claims Most ICOs Have Profited Off Se...
Luke attacks Roger, is proven wrong, deletes evide...


Since day one of trying my hand at hands-on investing, I've been coming across the topic of diversification a lot. And every time I'm in a discussion with someone about investing – and the concept of diversification is brought up – it seems that most people don't really know what it means. So here's a short guide I've put together to help myself understand it better. And through sharing, hopefully some of you can also gain some knowledge from it.
According to Investopia: Diversification is a risk management technique that mixes a wide variety of investments within a portfolio. The rationale behind this technique contends that a portfolio constructed of different kinds of investments will, on average, yield higher returns and pose a lower risk than any individual investment found within the portfolio.
Let's for example assume that your investment portfolio consists of one single company's stock. What whould happen to your investment portfolio if that company went completely bust? It would lose 100% of its value!
Now, if you had for example 20 different companies in equal proportion in your portfolio and that same single company goes bust, your losses are limited to only the 5% you had invested in that one stock.
With enough diversification, if one investment performs poorly over a certain period, other investments may perform better over that same period, reducing the potential losses of your investment portfolio.
Not all investors are in the accumulation phase of life. Some who are close to (or already in) retirement have goals oriented towards preservation of capital, and diversification can help protect your savings.
How does this work? As one approaches retirement, it is usually good practice to start moving some of your investments from high-growth (and thus high-volatility) investments to more stable (lower-volatility and thus lower-growth) investments. Typically this means moving partially from equities into bonds, REITs, money markets or other lower-volatility options.
When you are in retirement and need to draw down on your investments to sustain you, there are certain rules-of-thumb one can follow to help you figure out how much money you can safely withdraw without diminishing your capital too much. StealthyWealth wrote a magnificent blog post about using the 4% rule when calculating your safe withdrawal rates.
But how does diversification help in preserving my capital, you ask? If you have all your investments in the stock market and need to draw down, say 4% every year to live from, what would happen when there's a stock-market crash (a drop of more than 30% in the overall stock market)? If your portfolio goes down 30% and you keep on withdrawing 4% (or even a higher percentage, since the 4% you would withdraw now suddenly is also 30% less and isn't enough to sustain you), you'll rapidly deplete your investments and run the risk of running out of money in old age. And as mentioned above, even after the market picks up again, you'd need to make a lot more gains to make up for your losses, which might take years, even decades!
When diversifying into lower-risk (lower-volatility) investments, you're smoothing your portfolio out a little, so that market crashes won't affect you so much in retirement.
As already mentioned, stock markets are very volatile. Certain sectors within the stock market are also experiencing different levels of volatility. So do other asset classes available to one's portfolio.
A diversified portfolio means spreading risk by investing across different asset classes such as cash, fixed interest, property (REITs), local shares, international shares, commodities, etc. This lowers risk by spreading your exposure to asset classes which are not highly correllated. For example, different technology stocks (like Apple, Facebook, Microsoft, etc) will usually behave quite similarly and will create concentration risk for the investor. By diversifying into different asset classs, like Financials, Energy and Health Care, you lower your risk, should we experience another Dot Com bubble, for example.
A well diversified portfolio also spreads risk across countries. By investing globally, you lower your exposure to political and other localised risks, for example Brexit and its impact on investors in the UK. Or the political uncertainty around property ownership we're currently dealing with here in South Africa.
Another good way to diversify is across developed markets and emerging markets.
You can also spread your risk across currencies. As a South African, I can tell you all about currency volatility! Our Rand is one of the most volatile currencies on the planet, and having all your investments exposed only via this one currency can put you at very high risk. By investing in many different currencies, high volatility in any individual currency poses a lot lower risk to your savings.
When planning your diversification strategy, it is also a good idea to aim for assets with low correlation – or even no correlation – to help in lowering your portfolio risk.
What does this mean? It means, we're trying to find those classes of assets where a fall in one asset class won't be seen in another asset class. For example, the volatility of the whole S&P500 vs only the US REIT asset class, which has a correlation of only about 0.6, is one way to make use of low-correlation.
So now that you have a good idea of what the benefits of a diversified portfolio is, you want to know if there are any downsides to diversification?
The biggest downside is that a diversified portfolio will never be at the top of any "asset class chart" when ranked by performance. Because we're limiting our exposure (and risk) to any individual asset or class, we're also limiting our rewards. The NASDAQ for example has gained around 23.5% over the last 12 months, but a well diversified portfolio would not have seen that level of gain overall.
Investors can also become restless when they see the S&P 500 outperform their globally diversified portfolio during good times, or single stocks (like Netflix or Amazon) have stellar gains over a very brief period, which they didn't capitalize on as they could have if they had all their eggs in that one basket. This is true, a well diversified portfolio loses out on these individual super-performers, but remember, they also avoid the horribly poor ones as well!
Just think Steinhoff in recent South African history!
OK, so now that you know the good and the bad of portfolio diversification, how do you do it, you ask?
There's a lot of ways to approach this. You could do it quite manually and buy stocks in different companies, in different sectors, in different countries, buy and hold your own real estate, etc. But this is surely a very difficult and time-consuming approach. If you're just a DIY investor like me and you're looking for an easy, quick, cheap way to achieve good diversification, what I do is to just buy into an ETF or two that has high diversification built-in!
Take the SATRIX MSCI World ETF (if you're from South Africa like I am. Similar ETFs exist in most other countries, so do your own research) for example. It lets you invest in 23 developed markets across the world and holds equities in about 1500 different companies, in a wide range of different sectors!
By holding just this one ETF in a portfolio you're already very well diversified! That's great when you're still in the wealth accumulation phase, but what about when you're nearing retirement, as mentioned above, and you'd like to add less-volatile asset classes to your portfolio? Again, you can just add for example a Bonds ETF (like ABSA's Newfunds GOVI ETF) to add some ballast to smooth out your portfolio. As easy as that!
NOTE: I'm not a financial advisor, please discuss your portfolio with a professional! I'm just sharing how simple and straight-forward a portfolio can be while still being well diversified.
Over-diversification is a real thing!
As mentioned above, if one can find good diversification within a single product (like the MSCI World ETF), it keeps things simple. But sometimes we give in to FOMO and keep buying different ETFs when new products hit the market.
We end up with a portfolio that becomes difficult to analyse, more specifically, a portfolio where it's difficult to calculate what our exposures are to specific markets, asset classes, sectors, currencies and so on. This is because we lose track of what percentage exposure we end up with in each asset class, sector, country, currency or market, which we call concentration risk.
One last concept to take note of when you have a well diversified portfolio is the idea of rebalancing.
Say you have a R10,000,000 portfolio that's made up of 80% stocks and 20% bonds. You have R8,000,000 diversified into many stocks, and R2,000,000 invested into a basket of different government bonds. All nice and fuzzy!
Your portfolio is now worth R7,600,000 and the stock/bond allocation changed from 80/20 to roughly 74/26! You're now a lot more invested into bonds than what you initially intended!
To rectify this situation, an occasional rebalancing of your portfolio is required. What you do is to sell off just enough of your bonds and buying more equities to bring it back to the 80/20 allocation.
This isn't something that needs to be done regularly. As a matter of fact, it's better to do this only once a year or so. Selling and buying too often will put a drag on your portfolio's performance and should be avoided.
So that's diversification for you in a nutshell. It's not complicated and is easy to perform (especially nowadays with ETFs to our disposal), but it is a critical concept to ensure that you'll have a good chance of reaching your financial goals in the future.


Craig and Molly have similar stories. They both grew up in Christian homes. They both accepted Christ into their lives at a young age. They both were considered leaders among their peers, and in a relative world they have both made wise choices. Craig and Molly not only made a habit of waiting for God's best in their lives, but they also waited for each other. The Sanborns are in the minority of couples who married as virgins and racked up close to 65 years of virginity between the two!
Craig and Molly have not only united themselves through marriage – they have also united their passion for speaking about abstinence to youth and parents, and marriage to couples with a style and perspective that is incomparable and inspiring to all audiences. In addition to joint sessions with guys and girls, they love to get into deeper, gender specific issues. If the venue allows, they'd love to pair off – Molly with the gals and Craig with the guys. To have Craig and Molly speak at your next event, please fill out a contact form.
We are so thankful God orchestrated such an amazing love story, but it really pales in comparison to His love story. God sent His son to be the sacrifice for our sin so we could be forgiven and free to enjoy a relationship with Him – one that continues into eternity!
Check out the message below which showcases Molly's tricked out grocery cart and explains God's great love for you and his plan to give you abundant life.
Craig goes by many titles, but his favorite is "Dad". In addition to building forts and reading books , Craig spends his time as the high school youth pastor at Plymouth Covenant Church. He has a passion for teenagers and counts it a privilege to invest in their lives. Craig loves speaking and tag-teaming with Molly in ministry.
Craig grew up loving life in a small rural community called Cokato, which of course, is home of the annual celebration, the Corn Carnival. It was there that he grew up as a middle child in a family of five. Craig graduated fromDassel-Cokato High School in 1991 and pursued a career in broadcasting by graduating in the top two-thirds of his class from Brown College in the Spring of 1994.
After 2.5 years as a disc jockey and sports director at KILR radio station in Estherville Iowa, Craig followed the Lord's leading into a youth ministry position at Trinity Baptist Church in St. Paul, MN. As he began his new role as youth pastor, he also began to use his God given ability to speak to youth at camps, schools, churches and conferences across the country. In the Fall of 2004, Craig made the transition from a youth pastor to a full time youth speaker and communicator. After more than 20 years in youth ministry, Craig continues to pursue speaking opportunities while also investing into the local church as youth pastor.
When not playing Uno with his kids or spending time with teenagers you can find Craig sorting through his Zubaz collection, playing disc golf, playing Donkey Kong, listening to one of 11,000 songs currently on his I-Pod (which he paid for), or donning his new storm trooper suit.
Molly Sanborn (aka The Cheese Ball Chick) is a wife, mom, speaker, counselor, and lover of Jesus. Her creative ideas and infectious personality have been featured on several TV shows including Twin Cities Live and Wipeout. She loves to laugh and makes it her mission to help others find joy even in the midst of tough times.
Molly desires to be a role model to teens, proving that it is possible to live above the influence and have a blast while doing so! Her accomplishments are many. Molly served three years as high school class president, while maintaining a 4.0 and playing 3 sports. After high school, she spent one year in a discipleship training school through Youth With a Mission. During that year she did missions work in Turkey, China and Mexico.
Molly also loves any opportunity to encourage moms, dads, and married couples. As a mom of two and wife of almost 10 years, she "gets it" and loves encouraging others to go from surviving to thriving.
Molly has nearly completed her grad school journey of Marriage and Family Therapy and will be graduating in May of 2018. She graduated from North Central University in May of 2006 and holds a bachelors degree in Elementary Education. She was the captain of her college volleyball team, made it on the Dean's List every semester, and has traveled to 9 countries. She was awarded Elementary Education Student of the Year, and developed an award winning coffee shop, Overflow Espresso Cafe, at age 25. In 2015, Molly was honored by being selected as one of the "Ten Outstanding Young Minnesotans". Molly has taken many opportunities to lead and motivate and continues to do so as she travels and speaks influencing today's youth.
If you recognize Molly, perhaps you saw her on ABC's popular TV reality show, Wipeout. Or maybe you've seen her on YouTube or out and about with snacks on her back. She has become known as The Cheese Ball Chick as she brings "joy to the world one cheese ball at a time". If seeing is believing, then you'll want to check out her Cheese Ball Chick Gallery.
I (Molly) started journaling letters to God at the age of 12, and as I grew older, I often talked to God about the guys I liked and the husband I was hoping for. Below are excerpts from my prayer journals leading up to when I met Craig. I am choosing to make public some very private times with God so I can be an encouragement to you to go to Him with everything. "Dear diary" never worked for me, because seriously - your diary could give a rip! Just sayin' :). So I turned my thoughts, worries, and questions into prayers and watched how God brought peace, perspective... and eventually Craig - whahoo!!!
Lord, it's awesome! I seriously have NO desire for a boyfriend! Thank you for fulfilling me!
Lord, I want to be fully complete in You – lacking nothing. Please teach me how to do that.
Lordy, it's Valentine's Day and I am as happy as could be! Just You and me! Thank you for desiring good gifts for me and for giving me the desires of my heart (husband… hint! Hint! Just kidding!) You are all I want and if You throw a husband in the deal, well, Great! I love you!
I really am looking forward to marriage, but I don't want it to get out of control So please Lord, help me not focus on finding my guy or him finding me. Please help me redeem my time of singleness by using it for Your glory and an opportunity to be an example of a single woman of God.
Ok, so here we sit on Valentine's Day, 2002, and God, I am not the least bit lonely or sad. I am very content at where I am as far as being single. Please please continue to show me strategic and practical ways to take advantage of my singleness, Lord. Let this time in my life be used big time toward Your kingdom. Use me now in specific ways that You couldn't if I as married or had a boyfriend. Please allow me to minister to hurting hearts and lonely women.
Jesus, will you please help me guard this special time in our life and journey together? I ask more than ever now, that You will bring the person into my life in Your timing and without me having to work hard at it. My request of You is that You would be the match maker and let me know when the time and the man has come. God, some people may say, "Oh Molly, you have to do some work too. You can't just sit around and expect some prince charming to sweep you off your feet." Well, God, you know I am not just sitting around – I am running after you, Jesus, and building Your kingdom. I am busy with that, so I really don't have time to shop around! That's the honest truth, Lordy. For real! Like I've told You before – I don't know how you work things concerning marriage and who ends up with who, but I do know that you are my Daddy and You love me and want the best for me. So could You please scope out the territory and pick out a marvelous man after your own heart who You know would be the best for me and I would be the best for him. I BELIEVE You for that! How else could it be? So for now, Jesus, my portion is singleness, and I gladly drink this cup! Yum! Yum!
Last night, Mel and I prayed for our future husbands and reminded each other not to settle. I told You that I desire to be a wife that surpasses all my husband's wildest dreams and expectations. I want to blow his socks off! Yay! God, it is my desire to have a dynamic marriage – one that breaks the norm and all the stereotypes. Please prepare me to be the woman that request requires and send me a man that will fulfill the husband's role in that. I'm anxious, Lord, but willing to wait so that You can work in ways in me that You couldn't if I was not single, and also, I trust You are preparing my "H"!
Please continue to give me wisdom and discernment in this area – oh yeah – and PATIENCE – lots of it! Please prepare me to be an extraordinary wonderful wife. Please work in my hubby's life too. Give him the patience and wisdom to wait for me and not get distracted along the way.
It's so easy for me to worry about guys and marriage and stuff. So please help me focus, focus, focus and trust, trust, trust You to hook me up. I know I won't be disappointed. Yay!
I really am in no hurry. You've given me a desire to be single (for now!). Please help me stay true to You, Lord, and Your work. You've reminded me of a lot of things, Lord, that I've prayed for for quite a while. You've reminded me that my future hubby is WORTH THE WAIT!
God, the other night this good looking guy asked me out, and after we talked a bit, I turned him down. I don't regret it, but I must admit that I began to entertain the idea of what it'd be like to date all take all the guys who ask me out up on a date. No lie, it would be fun – but I gotta keep being reminded that in the long run, it's not the best. Too risky – hurt hearts, wasted time, temptation, etc. God, please help me stay true to You and my future love, my husband! Please help my husband guard his heart and have patience and the desire to wait for me.
I want to believe that there is an amazing man with passion for you and that I can be giddy about and be attracted to. Please help me hope and trust in You. Dad really encouraged me last night. He smiled real big and said, "Molly, I know the Lord wants to bless you with an amazing man because you have been so patient in waiting and have set your standards high." God, that really meant a lot to me coming from dad who sometimes teases me about being too picky.
Dad took me to Camp Snoopy on a date and when we were riding the orange Tumbler, I told dad that is how I want to feel about my future husband – the way my stomach was flipping around. That's what I hope and trust You for. Help me trust You!
I am totally seeing that my longing for a boyfriend/fiancé/ husband is increasing. A friend told me yesterday to go to You to fulfill that longing. That's so true and I know I've done it, but I think I need to learn how to do that even more. I want to be so content with You alone that a husband is just a marvelous additional gift – not a right. Please help me wait with patience and trust, and to not get sidetracked by looking to and fro! You know what Lord, I am super excited about talking to You and relying on You for wisdom and advice when I do meet that special guy. It'll be so much fun! I am excited to be giddy with You about it. For now, though, Lord, please help me make the most of this season of singleness. Help me be an example, Lord. I totally trust You with my future.
Lord, there are a lot of nice Christian guys, but not too many who are planning on serving You full time. I know they're out there, though. Please lead me to him – or him to me. I want to believe that I will know – that it will be obvious in the way he lives and talks. My desire is that my future husband's passion for You and devotion to You and Your kingdom will be so obvious that I don't need to wonder if it's there.
Please be giving my future husband heart preparation for me and contentment in waiting. Please give him wisdom and direction as he seeks You for advice on who he pursues and is interested in. Please save him and protect him from heartache and hurt. Help him focus on You completely. Please allow us each to be a gift to each other (from you!).
Please be strengthening my hubby as we're not yet together. Please help him set and maintain high standards, totally trusting me to You. And please help me do the same in the times I get restless or lonely (they've been coming more often!). Please help me continue to trust in Your timing and plan. Oh God – I'm excited! Yay! Please help me live my singleness to the greatest degree – to honor You with this time and live with no regrets.
Wow! You sure have been working on this heart of mine! I really want what You want. I really trust You. Please forgive me for worrying in the last few weeks and for entertaining ideas of finding my man. Lord, I genuinely am excited to live every day knowing it's from You and trusting You that you know the perfect day to introduce me to my hubby! Until then, Lord, I once again hold my hands and my heart up to You and ask You to take them and use them for Your glory.
My heart's desire is to marry a man passionate about speaking who I could support and partner with.
I am antsy to meet my man!
God I feel so free and ready to truly believe You for the incredible man I've always asked for! Ahhh… please help us be faithful and diligent as we wait for Your timing. Oh my goodness, God! I am so excited to meet my man!
God, I totally trust You for an amazing "catch" – one that I even believe will exceed my expectations! Whahooo!! Please give me (and him!) patience as we wait for You.
I gotta trust You for Your timing. Please be working on me and my guy – getting us ready for each other. Lord, I am so excited to fully, totally and completely give myself to my guy! I am excited to overwhelm him in a good way. Please help me and him be patient as we wait for You and are led by You. Thank you for protecting me so far in this area and allowing me to have such peace and no regrets or baggage as I move forward.
Dear Lord! This girl feels like she's gonna explode from all the stored up passion and giddiness! Oh, but what a fun feeling! To know I have EVERYTHING left to give one guy!
God, though You haven't spoken it to me as if it's definite, I think I've slowly become convinced that my story is going to be marrying someone with a past, perhaps a lot of baggage and then helping them work through it. But up in the tree house, it's like You hit me with this thought – it doesn't have to be like that – You could still choose to bless me with a man who doesn't have that story – and wow, God, even to think about marrying a guy who has waited for me was like a new thought for me! Wow, it was unbelievably refreshing to think about!
God, please teach me things. Grow me and shape me into a more godly woman. God I do trust you. I'm sure that You have a perfect, beautiful plan and have Your eye on a a fabulous man fro me to love and support, laugh with and change the world with. I do believe one day I'll stand before this man on our wedding day in my white dress and huge grin and praise and thank You for bringing me there. I know I'll even thank You for the journey, the process and the road that brought us to that place. It's just hard when I'm in the midst of the journey and unable to see ahead.
It's amazing and actually exciting how much I have to and want to give. Unhurt and with no pain or regret, I find myself uninhibited and eager to share myself with one man. I'm so grateful that is my story, Lord. Thank you for all Your guidance, Your protection, Your enabling and Your call for me to live with high standards and wait for Your best. There's that famous word that I am all too familiar with – WAIT! I giggle as I think about how many things I've waited for, and how most often it turns out to be well worth the wait. And then I know there are things that I should have waited for and haven't, and I wonder what blessings I've passed up because of my impatience. I don't want to do that on this one, Lord.
Ah Jesus! I love you! I am head over heals giddy about life and You! God, I am 26 and as single as ever and as happy and content as I ever could image being!! It's so great to be Yours and to be taken care of by You. You are a good God. You, my Lord, are all I need!! Thanks for letting me be so content in life in my singleness. It is amazing to have everything to look forward to. I am truly overwhelmed, Jesus. I love you. Thank you for filling me and letting me overflow on those around me. Amen! Amen! I love you.
Lord, I don't doubt Your nearness and even Your call for me to be speaking/ministering full time; but I have this ever growing longing to be doing it with someone.
Holy Spirit, I ask you to teach me and train me to be a woman whose priority is reflecting the beauty of the Lord, not the beauty of her body. A woman who is excited to keep her deepest secret for one man and to totally intoxicate him with the fullest extent of her beauty. Ahhh… that's gonna be fun!