The SKY65135-31 is a Microwave Monolithic Integrated Circuit (MMIC) Power Amplifier (PA) with superior output power, linearity, and efficiency. These features make the SKY65135-31 ideal for Wireless Local Area Network (WLAN) applications. The high linearity (low EVM) and high efficiency of this device makes it ideal for use in the transmit chain of WLAN access points or modems.
The SKY65135-31 is fabricated using Skyworks high reliability Heterojunction Bipolar Transistor (HBT) InGaP process, which allows for single supply operation while maintaining high efficiency and good linearity. The device is internally matched and mounted in a 20-pin, 6 x 6 mm Multi-Chip Module (MCM) Surface-Mounted Technology (SMT) package, which allows for a highly manufacturable low cost solution.
Please note: SKY65135-31 is not recommended for new design.


text:
Hands off my family
And my choice, Lance O'Sullivan. On 4th June, 2017, you appeared on Maori TV . You said words to the effect of, "If a person chooses not to vaccinate and gets that disease and it results in hundreds of thousands of dollars worth of treatment, as a member of society, I don't want to have to pay for that." So Lance, please answer these questions: If some of...
Dissolving Illusions.
Hilary Butler - Sunday, July 28, 2013
An Amazon.com company has announced today, the release of a new book called Dissolving Illusions, which reviews social history and it's impact on infectious diseases.
The coming adult needle cushion.
Hilary Butler - Saturday, March 19, 2011
Do you, like some other kiwis I know, roll up and get your five yearly "pneumonia shot"? You did know, of course, that it was useless, right? Of course not. I mean, if that was the case, your doctor wouldn't have given it to you, right? You did know that the Cochrane Collaboration, medical doyen of evidence based medicine, has stated that the polysaccharide pneumococcal vaccine is useless since...
Gardasil - in the quest for evidence.
Hilary Butler - Tuesday, February 08, 2011
This post is specifically for parents who believe their children have been damaged by Gardasil, and is an attempt to put in the public arena, information which has been conveyed privately, until now. This is information you need to know if your child is damaged, or had died after Gardasil. If your child has died. Current autopsy protocols in New Zealand are totally inadequate. Brain...
Secret documents show benzos cause brain damage.
Hilary Butler - Monday, November 08, 2010
Today was yet another embarrassing day for the "love a quick fix" medical system. The Sunday Independant in the UK revealed that secret documents marked "closed until 2014", showed that in 1982, the Medical Research Council was warned that benzodiazepines, which were marketted as "completely harmless", could cause brain shrinkage, and brain damage. The Department of Health, ver...
Starship Doctors slammed
Hilary Butler - Sunday, October 31, 2010
In the Sunday Star Times today, Judge David McNaughton delivered a swift message to Starship doctors about predictive and substandard medical care, and the presumption of guilt without good cause. The Judge found Famaile Lino not guilty of abusing his six month old child after Starship jumped to conclusions, and robustly defended their own preconceived mindsets. The L...
Twenty-five years plus is quite a long time....
Hilary Butler - Saturday, October 02, 2010
(By Peter Butler) Especially when it's been spent dealing with issues and systems governed by engrained mindsets, and heavily influenced by huge vested interests more interested in profits, than making available all of the facts ...without restriction; or providing customized care to unique individuals, rather than trying to fit everyone into their "one-size-fits-all" moulds. Health and...
Non-Evidence based Medicine: Part Three
Like many of my friends, there was once a time when I trusted the system; was ignorant about the actual lack of "evidence base"; and assumed that what I was told was right. Because of this naivety, I learned the hard way that the system can be wrong. Since then, I've done my best to keep myself out of this system, but when neded, I have never hesitated to confront the system&n...
H1N1 deaths: Auckland and Waikato ICU criminally negligent?
Hilary Butler - Friday, August 20, 2010
Are doctor prepared to their patients to die, rather than take note of either Allan Smith, or the known medical literature? Hence, this open letter to New Zealand Herald. Dear Sir, Your "in brief" report on page 5, in the NZH 20th August reporting the H1N1 death of a 53 year old man in Waikato hospital, (who had no pre-existing health conditions), and two Auckland men, 29 and 57. These de...
Gardasil is no sure fire protection
Hilary Butler - Saturday, June 12, 2010
Drs Hazel Lewis and John Holmes from the Ministry of Health (MOH), took an opportunity in the Herald letters to the editor to "swipe" an article called "Jab no surefire protection" which they maintain undermines an important health initiative. Never mind that the article was based on a Ministry of Health press release, and gave no negative information about the Gar...
The telephone rings. It's someone with a fully vaccinated child, whose kid has earache. Again. And was prescribed antibiotics. Again. Did the doctor run tests to see what it was? No. So let's toss an antibiotic napalm bomb into a kid, which will nuke what good bacteria there are that have a job to do in keeping bad bacteria under control. Dumb, dumb and dumber. But this is "in...
Little porkies and big porkies
Hilary Butler - Thursday, April 29, 2010
OPEN LETTER : Dear Drs Michael Osterholm, Kris Ehresmann and Ed Belangia; None of you get it, do you? You say you "know the idea that the flu vaccine doesn't prevent deaths in the elderly sounds almost blasphemous" and that you "didn't really want to believe it at first either," but that the new research is "incontrovertible"? The new research is no les...
Ignrance is not bliss: serious Hepatitis B vaccine questions
Recently, I was contacted by a young woman about to complete her midwifery qualifications. She had delivered a baby whose young mother was a hepatitis B carrier. This young aspiring midwife had recently returned a negative blood test for Hepatitis B antibodies, Immediately, concerns had been raised that she may have been exposed to surface antigen from the baby's ski...
GSK smacks UK Health Secretary's hand.
Here's how the story goes. In 2005 Glaxo Smith Kline, amongst other vaccine firms, threatened the UK Government, saying - "You've got to increase the use of ordinary annual flu vaccine in UK, or if a pandemic comes, we won't supply pandemic vaccine! So there." Fast forward to 2010. The UK Government wanted to back out on the 2009 H1N1 vaccine contract, but GSK refused.&nbs...
Swine flu vaccine produces non-functional antibodies in most people.
Hilary Butler - Saturday, April 24, 2010
You read that right. It comes right at the end of an astonishing article talking about how the 1976 Swine flu vaccine, (which never needed to be used in the first place, and was stopped because it caused serious neurological damage in recipients) produced really good levels of functional antibodies... However, the 2009 H1N1 vaccine did not. But here's the irony of it all... Point...
When Gardasil doesn't work, it works.
Of course Gardasil works in Australia. At least, it might when they've used it for long enough. But we won't see that for many years down the line, because most the girls who have received the vaccine, were probably already having sex, and therefore will have picked up HPV types. By the way, you're not supposed to know that HPV 16 and 18 can be got by a baby from it's mo...
Gardasil investigation - the fox in the henhouse?
From Bobby Cowans Press Relations: Members of TruthAboutGardasil.org and women around the world are disappointed with the panel of investigators selected to examine alleged violations of guidelines for conducting 'clinical trials', inadequate research, false advertising, along with various moral and ethical concerns in connection with a recent health 'demonstration project' involving...

redpajama_set_name: RedPajamaCommonCrawl


text:
Democrats don't get blank check from Black voters
Published on Thursday, 25 August 2016 14:42
Written by JESSE JACKSON
BY JESSE JACKSON
Donald Trump [recently] made a pitch for black votes in his own inimitable fashion. Speaking in a virtually all-white suburb of Detroit, he suggested that African-American communities are "suffering from Democratic control."
"What do you have to lose by trying something new like Trump. What do you have to lose?" he said to absent African-Americans. "You're living in poverty, your schools are no good, you have no jobs, 58 percent of your youth is unemployed."
This appeal for votes will fall on deaf ears. Most African-Americans don't live in poverty, even though too many do. Most have jobs, even though too many don't. More people in poverty are white, young and female. Trump has little relationship with the black community and isn't making any effort to create one. Most African-Americans consider him a bigot. (Four out of five African-Americans view him unfavorably, and slightly more think he's biased against minorities and women.)
President Obama won over nine of 10 African-American votes in 2012 when he ran for re-election. The black community is obviously proud of the dignity with which he has carried himself in office and the values which his family displayed. Trump earned our contempt for continuing to question Obama's birth certificate, trying to slur him as literally un-American. Not surprisingly, Trump polls in the low single digits — 2 or 3 percent — among African-Americans. He's running fourth behind the Libertarian and the Green candidates as well as behind Clinton.
The African-American vote hasn't always been Democratic. With Democrats anchored in the Jim Crow South, many African-Americans voted Republican before 1960. Dr. Martin Luther King's father was a Republican voter. But Democrats reached out to African-Americans, and Lyndon Johnson championed the end of segregation, the right to vote and more. Democrats didn't inherit black votes, they earned them. And recent efforts by Republican judges to disembowel the Voting Rights Act and by Republican state legislators and governors to limit the right to vote in ways that disproportionately impact African-Americans are teaching a new generation the same lesson.
African-Americans were a major part of the coalition that Hillary Clinton put together to win the Democratic nomination, and we will be a major part of the coalition she's putting together to win the presidency.
But Trump does have a point. African-Americans have suffered significantly from the stacked deck that characterizes our economy. We were the biggest victims of what the FBI called an "epidemic of fraud" in the housing bubble. We lost more ground than whites in the Great Recession. We suffer higher unemployment, a racially biased criminal justice system and inadequate public schools. Recent reports showing that poor African-Americans in Milwaukee and in other cities are living in more isolated neighborhoods, with more segregated schools, worse household incomes and greater incidence of concentrated poverty than that witnessed in Birmingham, Ala. in 1963 near the beginnings of the modern civil rights movement are clearly alarming.
As the first black president, Obama enjoyed a deep wellspring of support among African-Americans. We had his back. Clinton will not enjoy that unstinting loyalty. African-Americans will be making demands — as Black Lives Matter has already done — and looking for results. If disappointed, they may never get back to voting for a Republican Party that seems intent on locking them out if not up, but they may end up staying home in larger and larger numbers.
We need a targeted program to rebuild our inner cities. We need investment in jobs, housing and schools, as well as massive criminal justice reform. Hillary Clinton, periodically, talks about the Rep. James Clyburn's 10-20-30 plan. This calls for earmarking 10 percent of government spending for the 474 communities that have had 20 percent of the population in poverty for more than 30 years. As Clyburn notes, this isn't a blacks-only program. These communities are both majority Democrat and majority Republican; they are urban and they are rural. They include Appalachian whites, Alaskan Native Americans, urban Latinos and more. 10-20-30 won't solve the problems of Chicago's South Side or Milwaukee's north side, but it will demonstrate a clear concern for those struggling the most. That, along with reforming our criminal justice system and ending mass incarceration that has destroyed so many lives, would begin to revive hope. Without that, Clinton will find that our cities are tinderboxes, ready to blow.
Democrats are going to have to work to earn black votes again, not simply inherit them.
Category: Opinion


The General Secretary of the Yemeni Future Justic organization, Sayyid Hasan Ali al-Imad, stressed the book of the Supreme Leader of the Islamic Revolution, Imam Sayyid Ali Khamenei – may Allah protect him – "A 250-Years Old Man", had a great influence in changing the general public view. He considered that it is necessary to print and publish it on a large scale.
He turned to talk about the book by the Supreme Leader of the Islamic Revolution, Ayatollah Sayyid Ali Khamenei – may Allah protect him – "A 250-Years Old Man". He noted its prominent influence on the various layers of the Yemeni people, and its role in changing the view of the followers of Ahlul Bayt's – peace be upon them – doctrine there. This valuable book is comprised of a series of speeches by the Supreme Leader in regards to the political and Jihadi biography of the infallible Imams – peace be upon them – in an analytical framework, where his eminence has presented a contemporary vision of their blessed lives. He believes that this holy biography, even it differs from one Imam to another, but, in reality, it is a continuous and coordinated movement, that started since the tenth year of the Hijri calendar, and lasted 250 years, upon the minor Ghaybah. The political goal was one, which is establishing an Islamic government of Justice, described by his eminence as "The Alawi Government".


This first truly volunteer fire company in the US served as a model for others across the nation. Members, including Ben Franklin, purchased tools stored here in Grindstone Alley, helped provide a fire bell, buckets, and an engine for the city, and developed the idea of fire insurance.
This marker can also be identified by its HM code. You can use the hashtag #HM1KKC in tweets, and any other place where you might refer to this marker, including when searching on this site.


text:
est une série animée en douze épisodes de 25 minutes produite par Sunrise, réalisée par Tokita Hiroko et écrite par Tsunehisa Ito et Hiroko Tokita diffusé entre le  au  sur TV Tokyo.

En France, elle a été diffusée à partir de  sur Game One.

Trame et personnages
L'histoire relate la vie d'individus japonais normaux qui bascule quand ils apprennent être la réincarnation d'anciens guerriers Inca.

Miura Kyoji est un étudiant japonais sans problème, pacifique et moderne, il est passionné par la pratique du kendo. Il est la réincarnation de Bilka qui a empêché le guerrier Yawaru de détruire l'humanité avec une arme puissante.

Tate Masanari, maître (enseignant) et ami de Kyoji, est en fait le guerrier réincarné, nommé Incan Yawaru, qui veut détruire le monde pour le purifier. Tate peut devenir un monstre sans scrupule dès que l'âme du guerrier Yawaru s'éveille en lui. Pris par le désir de trouver l'arme mystique pour détruire l'humanité, Tate n'hésitera pas un instant à tenter de tuer son ancien ami Kyoji qui veut l'arrêter. L'histoire est sur le point de se répéter quand Kyoji découvre que Tate veut détruire le monde. Kyoji doit maintenant trouver les secrets liés à son passé, afin de contrer la menace que représente Tate et sauver l'avenir de l'humanité.

Kiritake Yuka, est la fiancée de Tate. Elle se révélera être la prêtresse Akurya, promise en mariage au cruel Yawaru, alors qu'elle avait des sentiments pour Bilka.

Épisodes
 L'éveil
 Rencontre sur les Andes
 L'appel des Hommes-âmes
 Les larmes, et puis l'adieu
 Irra Tezze
 Combat dans la ville morte
 La lumière du passé
 La terre promise
 La lumière qui chasse les ténèbres
 Au-delà de la lumière
 Vers la montagne du destin
 Et puis... l'avenir !

Repères

Culturels
 On peut noter que deux extraits de la série apparaissent dans le générique de la série Malcolm In The Middle.
 La musique d'ouverture de la série, Ai no Fugue, est très largement inspirée de la fugue en sol mineur, BWV 578 de Johann Sebastian Bach.
 De même, le thème principal de la piste Kuuchuu Shinden a été emprunté à la Symphonie du Nouveau Monde d'Antonín Dvořák.

Historiques
 Les Géoglyphes de Nazca n'ont pas été tracés lors de la période Inca, mais au cours de la civilisation Nazca.
 Le couteau cérémoniel Ylia Tesse représentant le Tumi est lié à la culture lambayeque même si quelques exemplaires retrouvés étaient utilisés par les Incas et la culture chimú.

Annexes

Notes et références

Liens externes

 Fiche de la série sur Planète Jeunesse
  Nazca sur Anime News Network
  Nazca sur THEM Anime Reviews

Série télévisée de fantasy
Série d'animation japonaise

redpajama_set_name: RedPajamaWikipedia


I'll admit it, I have a bit of a sweet tooth! So whenever I get a craving for something sweet and crunchy, I whip up a batch of these biscuits. They're the perfect healthy snack to add to my childrens' lunch boxes, and I keep one or two aside just for me. Tigernuts flour is naturally sweet, making it a great gluten free flour to use in sweet baked goods. You can typically find tigernuts flour in a health food store or high end grocery shop, but it can also be found online as a last resort. Of course, to add a bit of extra sweetness, I chose to add yacon syrup (which can be substituted with agave nectar). Ground turmeric adds a light golden colour to the finished product, and also enhances the biscuits' nutritional value. Turmeric is an excellent anti-inflammatory, so it can help prevent heart disease, cancer, and rheumatoid arthritis, amongst other diseases. It has a mild flavour, despite the fact that it's commonly used in curries, meaning it's an easy ingredient to sneak into most foods.
This whole recipe comes together in less than an hour (including chill time!), which means you can have delicious, healthy biscuits whenever you like. They can be kept in an airtight container for up to a week, making them a great option for snacks on the go. I hope you enjoy them as much as I do. Eat well to live well!
1. Combine the dry ingredients first, and then add the coconut oil, agave nectar and agar agar powder to make a dough.
2. Chill the dough in the fridge for 30 minutes.
4. Shape the dough into small rounds, and lay evenly onto a baking sheet lined with parchment paper. Bake for 15 minutes, and allow to cool fully before serving.


That's my blogging buddy Victoria from Apartment No 4 on the cover, I'm so chuffed for her too! I am so proud to be involved in contributing my tips to this publication alongside so many inspirational bloggers. The hacks, tips and ideas in this ebook have already changed the way that I look at my daily tasks so it's well worth a look. You can download the eBook for free right here.
And I've also contributed to an article on the LuxPad website. It's a feature on 'sublime summerhouses' and I wrote about my friend Lucy's colourful beach hut style garden building. Check out the full article about sprucing up your garden with these inspirational ideas here and have a read of Lucy's blog LucyLovesYa.com while you're at it.
In other news, I've been nominated for an Interior Blog Award and, while I know I don't really stand a chance against all those other fantastic interiors bloggers, it would be wonderful if you'd give me a quick vote. All you need to do is enter your email address here and your vote will be counted (and you'll be entered into a draw to win a £250 gift voucher too, bonus!) Thanks so much for supporting me!
I've also contributed to an article on how to decorate a small room to make it feel more spacious. My idea was to get everything off the floor, as seeing more of the floor gives the illusion of more space in the room, no matter how tiny! 55 interior design bloggers have added their tips to this round-up so have a read if you're planning to decorate a small space. My buddy Victoria from Victoria's Vintage blog contributed to this article too so be sure check out her blog too.


text:
Q: current() VS list() on each() I'm trying to get rid of deprecated code on a PHP7.2 web server by replacing the following code:
list(, $block) = each($this->blocks);

with that:
$block = current($this->_blocks);

I am not expecting any differences. Tests on simple arrays return the same results. Can anyone sees how it could be a problem ?

redpajama_set_name: RedPajamaStackExchange


REDMOND, Wash., Jan. 11 /PRNewswire/ -- There has never been anything like it. Independent U.S. sales figures released by the NPD Group today confirmed a red-hot launch for the new Wii(TM) system by Nintendo, and the crowning of the portable Nintendo DS(TM) as the best-selling video game device of any type for both the holidays and all of 2006.
and historic sales of accessories, including more than 1.5 million extra controllers for Wii alone, Nintendo recorded the most successful across-the-board holiday performance in U.S. video game history.
a million in just the 44 days between U.S. launch and year end.
DS achieved full year sell-through of 5.3 million.
Train Your Brain in Minutes a Day, also were top-10 sellers for the year.
accounted for more than half of all video game hardware systems sold in America.
Earlier this week, Nintendo increased its projected worldwide financial performance for the year ending on March 31 to be the best in company history.
"Millions of players, both hard-core and brand-new, are already enjoying the unique entertainment provided by Wii, but demand for the system remains incredibly high," says Nintendo of America President Reggie Fils-Aime. "So our work is just beginning. We're dedicating ourselves to maximizing both manufacturing and distribution full bore throughout 2007, to make sure everyone who wants to experience the future of video gaming can do so just as soon as possible."
The momentum behind Wii will continue unabated through the early weeks of 2007, with retail debuts of WarioWare(TM): Smooth Moves, Wii Play(TM) and Mario Party(R) 8 from Nintendo; a host of third-party releases; and a steady stream of classic games made available via download with the Wii Shop Channel.
The worldwide innovator in the creation of interactive entertainment, Nintendo Co., Ltd., of Kyoto, Japan, manufactures and markets hardware and software for its Wii(TM), Nintendo DS(TM), Game Boy(R) Advance and Nintendo GameCube(TM) systems. Since 1983, Nintendo has sold nearly 2.2 billion video games and more than 387 million hardware units globally, and has created industry icons like Mario(TM), Donkey Kong(R), Metroid(R), Zelda(TM) and Pokemon(R). A wholly owned subsidiary, Nintendo of America Inc., based in Redmond, Wash., serves as headquarters for Nintendo's operations in the Western Hemisphere. For more information about Nintendo, visit the company's Web site at www.nintendo.com.


text:
Enhanced Asset Management Suite (EAM)
Enhansoft Reporting (ER)
Warranty Information Reporting (WIR)
Custom Inventory Reporting
Free Giveaway
How to Spruce-up Your SCCM Reports by Adding Page Numbers
By	Garth Jones
In this day and age, why would you need to add page numbers to a report? Well, the first reason should be obvious. Even in a "paperless society" sometimes a report needs to be printed, so if it does, adding page numbers is a great way to keep the pages of the report organized. Not to mention that it also tells you how many sheets of paper you'll need to print it on. The second reason why adding page numbers to a report makes sense is not so obvious because it has to do with a SQL Server Reporting Services (SSRS) issue.
Before getting to that issue, though, let me tell you that I have seen my fair share of multiple-paged reports printed time and time again. Then you know the drill. Page after page is flipped as the information is reviewed. A tidbit of data is flagged here and there, and maybe even pages are put into separate piles. This is all fine until one of your colleagues walks past the sheets of paper and they all go flying! It can be frustrating trying to reorder the pages correctly and sometimes simply reprinting the report is faster than trying to figure out which page goes where.
Instead of wasting paper and toner, try adding page numbers. Many moons ago, when I attended college we used punch cards. Yes, punch cards! One day I dropped a stack of them (this was for a cool assignment using PDP-11). Needless-to-say the cards were not in order when I picked them up. Frustrated, I turned for advice to my Dad who worked with punch cards a lot. He told me to write a number on each card (similar to page numbers) and this way if they ever got out of order again, I could easily reorder them without too much trouble.
SSRS Issue
Take a look at these two reports. Can you see how the page numbers are displayed differently?
I've enlarged and flagged the relevant section. The first report says that the current page is 1 of 2 ? and the second report says that the current page is 1 of 2. What does the question mark ? in the first report indicate? Does it mean anything?
In actuality, this report is 39-pages long, so you can't ignore the question mark.
Why, then, does this happen?
Here's a look at page 39.
Usually page numbers appear either in the top right or somewhere along the bottom of the page. Notice, however, in this example that there are no page numbers on the report itself. Yes, page numbers appear in the SSRS toolbar, but not on the report itself.
Let's look at the second report. The page number information, Page 1 of 2, appears within the footer. When this information appears in the report itself, it enables SSRS to detect the number of pages in a report. The correct information is then displayed on the SSRS toolbar.
Instead of guessing the number of pages in a report from the SSRS toolbar on the first screen, try adding page numbers to a report! This is one of those little things that can really make a difference. A little later I'll show you how to add page numbers within a footer, but first I'll show you how to add a footer to a report.
How to Add a Footer to a Report
This report doesn't have a footer, so in order to add one I first have to ensure that the design tab is active. Next, I click on the Report tab and select Add Page Footer. Wasn't that easy?
You can see in the screenshot above that I highlighted the footer area after adding it.
Adding Page Numbers to a Report
Within the footer area, add a text box.
Right-click on the text box and select Text Box Properties…
In the expression text box, paste the expression listed below and then click OK.
=String.Format("Page {0} of {1}", Globals!PageNumber,Globals!TotalPages)
Page Number Expression
What does this expression do? In simple terms, it displays the current page number followed by the total number of pages, i.e. Page 1 of 2. You can see what I mean when you view the report screenshot below. Additionally, it uses built-in global variables for the current page number and the total number of pages. These are automatically populated when the report is generated. I intend to write more about these global variables in future posts.
If you have any questions about adding page numbers to SCCM reports, please feel free to contact me @GarthMJ.
How to Spruce-up Your SCCM Reports by Adding Page Numbers2018-08-222019-07-18/wp-content/uploads/2018/06/enhansoft-logo.pngEnhansofthttps://www.enhansoft.com/wp-content/uploads/2018/07/Adding-Page-Numbers-Enlargement-Area_thumb.jpg200px200px
Enhansoft Reporting for SCCM (ER)
How to Create Cascading Prompts
How to Create a Collection Prompt Query
Accessing ConfigMgr Reports – Browser or Console
Software Updates Dashboard
How to Determine the Microsoft Azure Tenant ID
Free Tools and Reports
© 2006-2021 Enhansoft. 1 Wheeler Street, Suite 200, Ottawa, ON, Canada, K2J 3C2.
How to Leverage the Interactive Sorting Feature in SCCM ReportsConfigMgr, ConfigMgr (Current Branch), ConfigMgr2007, ConfigMgr2012, ConfigMgr2012 R2, Configuration Manager, How-To, Queries, Query, Reports, SCCM, SCCM (Curent Branch), SCCM 2007, SCCM 2012, SCCM 2012 R2, SQL Server, SQL Server Data Tools, SQL Server Reporting Services, SSDT-BI, SSRS, System Center Configuration Manager, Tips
When Was This Report Created? It Needs an Execution Date.ConfigMgr, ConfigMgr (Current Branch), ConfigMgr2007, ConfigMgr2012, ConfigMgr2012 R2, Configuration Manager, How-To, Reports, SCCM, SCCM (Curent Branch), SCCM 2007, SCCM 2012, SCCM 2012 R2, SQL Server Reporting Services, SSRS, System Center Configuration Manager, Tips


Thermo Scientific offers a line of specialized ion sources, alternative dissociation techniques, probes that optimize MS workflows and performance to help you obtain the most sensitive data possible.
Direct ionization technique minimizes sample prep, avoids chromatographic separation to obtain mass spectrometry sample analysis in 1-2 minutes.
Field asymmetric ion mobility spectrometry provides unsurpassed LC-MS instrument selectivity and detection limits for proteomics applications.
Create a seamless CE/ESI-MS workflow with a single microfluidic device which rapidly prepares, separates, and electrosprays biological samples into your mass spectrometer.
Optimize nanoflow LC-MS with Thermo Scientific EASY-Spray IMAC-10 LC Columns, which are temperature-controlled for maximum reliability and performance. These analytical columns are compatible for use up to 1000 bar (15,000 psi) and have an integrated column/emitter design that eliminates dead volumes.
For applications ranging from conventional analysis of small molecules and polymers to sensitive data collection from trace or nonpolar compounds in complex matrices, Thermo Scientific offers a wide range of ion sources suited to various analysis modes including high-resolution accurate-mass (HR/AM) mass spectrometry and paper chromatography.
EASY-Spray C18 LC Columns Optimize nanoflow LC-MS with Thermo Scientific EASY-Spray IMAC-10 LC Columns, which are temperature-controlled for maximum reliability and performance. These analytical columns are compatible for use up to 1000 bar (15,000 psi) and have an integrated column/emitter design that eliminates dead volumes.
EASY-Spray Transfer Lines Fitted with Thermo Scientific Dionex nanoViper for easy installation, the EASY-Spray Transfer Lines help introduce external tuning compounds without needle alignment and handling. This no-separation sprayer enables you to tune your mass spectrometer quickly and easily.
Electron Transfer Dissociation (ETD) For improved sequence coverage and post-translational modification analysis of peptides and proteins, choose the ETD option for peptide fragmentation. This option can be used instead of, or in conjunction with, collision-induced dissociation (CID) on LTQ series linear ion traps and hybrid ion trap-Orbitrap mass spectrometers.
FAIMS Pro Interface For challenging bioanalytical applications, including the accurate determination of pharmacokinetic parameters, high-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) can be used to optimize ion selectivity and reduce chemical backgrounds during mass spectrometry assays. FAIMS successfully prevents interferences from co-eluting compounds and increases signal to noise ratios by 100-fold or higher.
VeriSpray PaperSpray Ion Source Improve turnaround time, reduce cost per test and minimize sample preparation with a fully automated, fully integrated, high-throughput, direct ionization technique used with our newest triple quadrupole mass spectrometers.
Heated Electrospray Ionization (HESI-II) Probe Enhance your LC-MS assays up to eight times by adding a HESI-II probe to your Thermo Scientific Ion Max ion source. The HESI-II probe provides better desolvation and improved nozzle performance, and is already a standard feature of many Thermo Scientific mass spectrometers.
MSPac™ DS-10 Desalter Cartridge Reduce interference from salts and contaminants commonly present in biological samples used in clinical research, biomarker discovery and systems biology applications with the MSPac DS-10 Desalter Cartridge. This cartridge contains 5um porous copolymer beads for use in reverse phase LC-MS assays.
MSQ Accessories Ensure reliable performance of your Thermo Scientific MSQ or MSQ Plus LC-MS system with our preventative maintenance kits and accessories.
MSQ 18LA Nitrogen Gas Generator Ensure the highest productivity from your mass spectrometer with the Thermo Scientific MSQ 18LA Nitrogen Gas Generator. This generator operates 24 hours per day, seven days per week in either electrospray ionization or atmospheric-pressure chemical ionization (APCI) mode to match even the most demanding workload.


text:
Every Soul a Star
By Wendy Mass
Curriculum Subject: Friendship, Self-Discovery
Educator Guide
At Moon Shadow, an isolated campground, thousands have gathered to catch a glimpse of a rare and extraordinary total eclipse of the sun. Three lives are about to be changed forever.
Ally: Ally likes the simple things in life-labyrinths, star-gazing, and comet-hunting. Her home, the moon shadow campground, is a part of who she is. She refuses to imagine it any other way.
Bree:
Popular, gorgeous (everybody says so), a future homecoming queen for sure. Bree wears her beauty like a suit of armor. But what is she trying to hide?
Jack: Overweight and awkward, jack is used to spending a lot of time alone. But when opportunity knocks, he finds himself in situations he never would have imagined.
Told from three distinct voices and perspectives, Wendy Mass weaves an intricate and compelling story about strangers coming together, unlikely friendships, and finding one's place in the universe.
★ "Readers who like… Lynne Rae Perkins's Criss Cross and Jerry Spinelli's Stargirl books will also enjoy this compelling and through-provoking story." –School Library Journal
"Confirming her mastery of the middle-grade novel, Mass combines astronomy and storytelling for a well-balanced look at friendships and the role they play in shaping identity." –Publishers Weekly


This is where a segment of the so-called alternative media can play a valuable propaganda function for Western powers. Because such media are supposed to be independent, critical, non-corporate, the public tends to consider their reports as objective and unbiased. One such "alternative" news service is "Democracy Now" hosted by Amy Goodman. Goodman is seen as something of a campaigning critical journalist shedding the light of truth on the depredations of the US government, corporations and the Pentagon. But a closer look at what Goodman's "Democracy Now" is reporting on Syria shows that the purported critical broadcaster has become a purveyor of Western government propaganda. While the mainstream media's propaganda function is obvious to the informed public, Goodman's "Democracy Now" plays a more subtle role. Camouflaged with the trappings of critical, independent journalism, "Democracy Now" serves to sow powerful seeds of misinformation in a way that the "compromised" mainstream media cannot.
Every possible smear campaign against the Assad government has been indulged in and indeed fabricated. From the alleged killing of innocent civilians by the national armed forces, to the perpetration of massacres by pro-government militias, to self-inflicted car bombs in urban centres by Assad secret services, to the feckless shopping habits of the president's wife. Russia Today, Press TV, Der Spiegel, the Frankfurter Allgemeine Zeitung, the Vatican News Service (Agenzia Fides), to name a few, have been honorable exceptions in mainstream media journalism for conveying a more accurate picture of what is really happening inside Syria – showing that "protesters" are far from peaceful civilians, and much of the violence is actually stemming from Western, Turkish and Arab-backed mercenaries that have infiltrated the country. As the facts of US and NATO-backed violence in Syria become more transparent and harder to conceal owing to the sheer volume of covert involvement, the Western public has rightly become more skeptical about what the mainstream media outlets are telling them. Indeed, the blatant misinformation and lies that are being sold as journalism is increasingly seen as contemptible.
The Houla massacre on 24 May is a case in point. The BBC and other mainstream media outlets have been shown to be outrageously wrong in their initial rush to blame the atrocity on Syrian government forces when the evidence has slowly emerged that it was most likely the grisly work of Western-backed mercenaries.
These reports were known to the alternative media. "Democracy Now" chose to ignore them.
Goodman also indulged in the overblown casualty figures from dubious Syrian opposition sources as if they were verifiable accurate data. She even sounded like Hillary Clinton in talking up the "defection" of the hapless former Syrian Brigadier General Manaf Tlass as "significant" when informed sources discount that news as a minor irrelevance.


text:
Closing the Division of Juvenile Justice
With the adjournment of the annual state legislative session, we thought we'd share with you some of the bills that made it to the governor's desk. We would also like to focus on a significant change to juvenile justice in California.
Even though state lawmakers had to contend with conflicts relating to COVID-19, some interesting pieces of legislation made it to Governor Gavin Newsom. Such bills include but are not limited to legislation that would shorten probation terms (Assembly Bill-1950), enable parolees to earn a quicker end to supervision (Assembly Bill-2342), and create a state-level re-entry commission (Senate Bill-369).
In May, Governor Newsom proposed closing the Division of Juvenile Justice (DJJ) and all its remaining state facilities in favor of local alternatives as part of the 2020-21 state budget. The announcement was met with sharp criticism; however, it looks like the novel plan may come to fruition.
State lawmakers were able to get a trailer bill, AB-1868: Juvenile Justice Realignment, to Newsom's office in the session's final hours. An agreement was struck laying the groundwork for a new kind of juvenile justice, one that shifts the focus away from incarceration in favor of rehabilitation.
The Office of Youth and Community Restoration
In the place of the DJJ, the new Office of Youth and Community Restoration, a part of the state's Health and Human Services Agency, will "have critical responsibilities to oversee county juvenile justice systems, administer funding, and ensure local policies and practices reflect the state's priorities for children and families." Grants will be given to counties to provide custody and supervision.
"That kind of systemic transformation is exactly what I think we're learning needs to happen in this time when you've seen much tumult around how the criminal justice system operates and whether it's fair and equitable particularly as it relates to the treatment of kids of color," said Chet Hewitt, of the reform group California Alliance for Youth & Community Justice.
There are currently four DJJ facilities that house about 775 youths, according to The Imprint. The majority are at three youth prisons, and 70 are at the Amador County fire camp, which trains youths in firefighting. Beginning next July, the state will no longer accept most youth offenders. The agreement made with the governor's office also raises the age to 25 for some youth to remain in the juvenile justice system.
Next year, local governments will be tasked with detaining youth offenders in county detention centers. Those currently serving time in state-run juvenile detention centers will remain in state custody until their time is served or they reach age 25. The Amador fire camp will continue training youths under the new system.
Orange County Juvenile Defense Attorney
Please contact the Law Office of Katie Walsh if your son or daughter faces legal difficulties or school expulsion. Attorney Walsh has significant expertise in these matters and can advocate for your family. You can reach us at 714-351-0178 for a free consultation.
Our thoughts and prayers go out to everyone affected by the many forest fires raging across the state. We hope that everyone finds themselves safe.
Labels: AB-1868, bill, DJJ, Governor Newsom, juvenile, juvenile defense, juvenile justice, legislation, Office of Youth and Community Restoration, youths


Microsoft Azure Active Directory Premium – providing you with granular reporting, self service password reset and multi factor authentication.
Microsoft Intune – providing you with class beating Mobile Device Management, including conditional access features and System Center integration.
Microsoft Azure Rights Management – lock down your corporate data, whether in the cloud or on premise with Azure RMS.
I have had a play with all three of these products and I must say that there is a lot of very cool stuff in there. By the end of our week of testing, we had all the bells and whistles going, and the level of functionality which we had achieved was fantastic. Device enrolment was easy, MFA worked a treat and my test data felt like it was covered in bubble wrap.


text:
Умлаут — діакритичний знак
 Умлаут — фонетичне явище сингармонізму в деяких мовах (германських, кельтських, а також уральських і алтайських).


text:
The Guy Is Now the Guy Behind the Guy
Scott Pioli was one of football's most celebrated minds during his time in New England. After his career derailed in Kansas City, he's gotten back on track in Atlanta — where he's working for his former employee and preparing to face his former team.
By Katie Baker Feb 2, 2017, 9:15am EST
Share All sharing options for: The Guy Is Now the Guy Behind the Guy
(Getty Images/Ringer Illustration)
Scott Pioli had been married to Bill Parcells's daughter, Dallas, for less than seven months when he sought permission from his father-in-law, who was also his boss, to go ride with the enemy. It was 2000, and thanks to some beef with coach-turned-GM Parcells, Bill Belichick had just jumped ship from the New York Jets, where he'd been the head coach for one day, to the New England Patriots, where he would become the man for eternity. Belichick offered Pioli, who'd served as the Jets director of pro personnel, a plum player-personnel job in New England. Parcells allowed it, and Pioli not only won three Super Bowls with the Patriots — he became one of the sport's most sought-after minds.
In Sporting News polls voted on by league executives, Pioli won back-to-back NFL Executive of the Year honors for the 2003 and 2004 seasons. By the end of the aughts, he was a popular fixture on various "best of the decade" lists. (The rationale for his selection as ESPN's "Personnel Man of the Decade" was: "Everyone you talk to across the league mentions Scott Pioli first.") It was the culmination of a lifetime of football geekdom: In high school, in Washingtonville, New York, Pioli would hang a bedsheet on his wall and project opponent game film onto it. As a student at Central Connecticut State, he frequently drove to Giants practice, where he was introduced to Belichick, with whom he hit it off. And after stints with the Browns, Ravens, and Jets, he went to New England, where he was instrumental in drafting and advocating for Tom Brady and in finding unsung players at good values who could assimilate into The Patriot Way.
This Is What Separates Bill Belichick From Every Other NFL Coach
This weekend, he'll again be in the building as the Patriots play for a Super Bowl — but this time, he'll be fighting to thwart Belichick and Brady, not assist them. As Pioli, now the Atlanta Falcons assistant general manager, told the New York Daily News this week, "There is no percentage of me that wants the Patriots to win. That's zero percent."
Both Pioli and Falcons general manager Thomas Dimitroff are former New England staffers; Dimitroff was the Patriots director of college scouting from 2003 to 2007. They both left to take GM jobs: Dimitroff with Atlanta in 2008, Pioli with the Kansas City Chiefs in 2009. But while Dimitroff has remained with the Falcons since then, Pioli's once-celebrated career only recently got back on track after a derailment in Kansas City, where he was fired after four turbulent seasons. Now he's back on the staff of a Super Bowl contender, serving as the assistant to a man who was once his employee. For Pioli, though, remaining mostly behind the scenes might have been the best way to move his career forward again.
Pioli's whole life revolved around football. So he wasn't prepared for so many off-the-field considerations to enter his orbit once he took on the Chiefs GM role. "Less time is spent doing the job you fell in love with," he wrote in Sports Illustrated's MMQB in 2013, shortly after Kansas City had sacked him. Pioli had been obsessed with "the purity of the game and competition," he wrote, focused on the nitty-gritty details of a player's release or explosive speed, and so he was surprised by how panoramic his vision had to become.
As GM, Pioli wrote in SI, he wound up spending weeks' worth of time doing things like addressing Twitter drama or advising a player on how to confront friends who were raising aggressive dogs on his property. To a self-described "old football soul" like him, this was a challenge. Meanwhile, problems were mounting on the field as well. Soon after coming to the Chiefs, Pioli made three significant moves: He hired Todd Haley as head coach, he signed Brady's New England backup Matt Cassel to a significant contract, and he picked defensive lineman Tyson Jackson in the 2009 draft at third overall. None of them worked out as planned, and Kansas City finished in last place in its division in 2009, 2011, and 2012. (The Chiefs' record that last year was 2–14.)
Pioli's relationship with Haley deteriorated to the point that, in 2012, the Kansas City Star ran a long, scathing feature headlined "Arrowhead anxiety" in which, among many other things, an increasingly paranoid Haley alleged that the team had tampered with his cell phone. (He would be fired four days after telling that to reporter Kent Babb.) Cassel, whom Pioli drafted in the seventh round in 2005, had transcended his career backup role when he successfully filled in for an injured Brady in 2008, and he became one of 14 former Patriots players and coaches whom Pioli hired early on in his Chiefs tenure. But Cassel's play was unable to match his overly optimistic six-year, $63 million contract, and by 2012 fans were booing him during the introductions of a celebrity softball game and he was embroiled in a quarterback controversy with Brady Quinn. Jackson's career was quickly surpassed by many defenders taken behind him in the draft, from Clay Matthews to Brian Cushing.
And all of this was rendered frivolous when tragedy struck in December 2012. Jovan Belcher, an undrafted Chiefs player, shot and killed his girlfriend during a dispute, drove to the Chiefs' practice facility, thanked Pioli for taking a chance on him, and killed himself in front of Pioli and coach Romeo Crennel in the parking lot. (An autopsy showed that Belcher had signs of CTE.) "The way I've looked at it," Pioli would later tell Dan Patrick, "I was not a victim that day. I saw something awful, but the reality is there are other victims out there … someone lost their daughter, someone lost their son."
Pioli's candid conversation with Patrick was a rarity for him; one of the lessons he picked up from Parcells and had reinforced by Belichick was that it was best to stay out of the spotlight. While Pioli was once voted high school "class clown," and while he was a curious soul who loved to cultivate relationships with leaders in other sports, like then–Red Sox manager Terry Francona, he wasn't the type to shoot the breeze with the media. This was particularly true in Kansas City, where, he later admitted in his MMQB piece, he "was naïve to not understand that part of the job description of a GM included being one of the media faces of the organization." But to disgruntled fans, his ongoing silence spoke volumes. In October 2012 they had turned to the age-old protest method of hiring planes to tote banners demanding his firing, and they got their wish at the end of that season. "There are a million jokes we can make here," read an incredulous piece in the Kansas City Star when it was announced that Pioli had taken a job as an NFL talking head.
"Is Scott Pioli going to go in there and ruin that franchise like he did Kansas City?" former quarterback and current CBS personality Boomer Esiason ranted on Atlanta radio in January 2015 in reaction to news that Pioli would run the Falcons' next draft. "I would hope and pray that Scott has figured out what he did wrong in Kansas City and how he just completely turned that franchise upside down and inside out."
With the eighth pick in that draft, the Falcons took linebacker Vic Beasley, who this season led the league in sacks, and with the 42nd pick, they took cornerback Jalen Collins, who forced and recovered a fumble in the Falcons' NFC championship win over Green Bay. They are two of many key young Falcons players whom Pioli, in his short tenure, has already had a hand in selecting. Esiason predicted at the time that Pioli's new duties would crowd out — or "cut off at the knees," as he put it — Dimitroff's role in the organization. Instead, everyone appears to have stepped back and created more room for debate — and that includes second-year head coach Dan Quinn, who has forged a strong, collaborative relationship with the front office.
The Falcons' Unconventional Motivational Strategy
"The interesting thing," Pioli told NFL.com last week, "is Thomas [Dimitroff] and I come from a background where we were looking stylistically for a completely different type of player" than Quinn was interested in. Whereas in Kansas City Pioli and Haley clashed when they failed to see eye-to-eye, in Atlanta Pioli instead "rewrote a lot of the things we scouted," after speaking with the coaches, looking not just for the "bigger, stronger guys" he preferred but for those with good functional speed. He pointed out linebackers like De'Vondre Campbell and Deion Jones, both of whom Atlanta drafted before this season, as examples of players whose speed fits in with Quinn's system but also have Pioli's preferred size.
The Falcons have played the Patriots only four times in the Brady-Belichick era, and have lost every time. There isn't much on-field history, leading up to this game, to parse and mine. Instead, the more interesting for-old-time's-sake matchup is the one between Belichick and his longtime, long-lost disciples. If the Falcons upset the Patriots — and even if they don't — Pioli has begun building anew a case for himself as a keen football mind with an ability to identify talent and a willingness to admit that he's made some mistakes. Given the Falcons' success, he could be in line for another GM opportunity elsewhere soon. For now, he has found a pretty good role on what may well be remembered as a pretty great team.
Next Up In NFL
The Worst Bets for the Divisional Round
The NFL Food City Playoffs: Delicious Divisional Round—AFC Edition
How Rasul Douglas Finally Got His Chance to Show Up and Show Out
The Titans Are Finally Healthy. But Will That Be Enough for Them to Make a Run?
The NFL Divisional-Round Entrance Survey


Some of us at The Edge have put our plans of venturing into politics on hold, what with the mandatory declaration of assets, pay cuts and so on. It seems being a journalist may not be so bad after all — we don't have that many assets to declare and our monthly pay is, well, not worth declaring.
At the rate things are going, don't be surprised to see Tun Dr Mahathir Mohamad in his Bata slippers on the bus going to the MRT station or heading for a sale at Kamdar near Jalan Tunku Abdul Rahman for Raya shopping.
Back to our dreams ... a particularly fabulous one was to be an adviser to politicians and take home up to RM200,000 a month. That works out to RM2.4 million a year, which is much more than what some heads of government-linked companies make. And they have to work really hard and be accountable to shareholders and the government.
Sigh … gone are the days of easy money, of little work but drawing a fat salary, and best of all, no deadlines. In the past, it seemed that one didn't really need to be a graduate to become a politician. Occasionally, you had to stand up in parliament and reply to some questions from the opposition. The most important thing was to make an appearance in your constituency every five years and make promises you won't keep, and maybe give out goodies, including ang pows.
But after May 9, the rules of the game have changed drastically. The current lot have killed the fun in politics by actually working, and keeping the rakyat at the top of their priorities.
And they seem to be working really hard. Finance Minister Lim Guan Eng holds a press conference every seven minutes. And if he is working, Tony Pua and Ong Kian Ming must be slogging away as well.
Anyway, let's not talk about any comparison with Ahmad Mazlan and fried rice, etc.
Whatever, journalism just doesn't allow one to experience the finer things in life — Cohiba Behike, Chateau Lafite or Krug wine and bubbly, and not forgetting Brioni suits and Bruno Magli shoes. The list is very long, and I am sure the women have Birkin bags on the list as well. By the way, how do you pronounce Hermès?
Okay, maybe I had better stick to brands we can pronounce, such as Bonia and Clarks.
But then again, while there are many good things happening, there are sudden shocks as well.
The planned revival of the national car, for instance. The knee-jerk reaction was, "Oh no, not again".
What's the new car to be called? Maybe we could call it Kereta Anda, Rakyat 1Malaysia, or Kar1 Malaysia, for short.
We'll set up the plant in Terengganu so that the RM60 billion East Coast Rail Link will actually find some use and not remain a white elephant. What is it the mat sallehs say, kill two birds with one stone?
After 25 years of Proton, billions in grants and terrible models such as the Juara, it is no wonder the national car plan did not whip up much enthusiasm. But then again, it depends on who you ask.
Speak to the vendors, who had a good thing going for a long while but were forced to up their game recently, and they will tell you Proton was a runaway success.
But ask regular folk and it is likely you may be called some profanity not fit to be published in this paper, and you may have to listen to gripes about water in the headlights and power windows that do not function, especially when it is raining.


Worksheet : Short Reading Selection With Comprehension Questions. Exploring Science 7 Worksheets Answers. Grade 4 History Transport Worksheets. 4th Grade Algebra Worksheets.
Published at Monday, April 15th 2019, 05:41:12 AM. Worksheet. By Villette Fabre.
Tags : basic English worksheets for adults. grade 3 math lessons. fun math practice. 4th grade textbooks. comprehension passage for grade 3 with questions. math resources ks2. printable short stories for 3rd graders. common core 4th grade. multiplication worksheet generator free. elementary worksheets.
Thoughts on Short Reading Selection With Comprehension Questions?


text:
package ksdn

import (
	"encoding/json"
	"fmt"
	"net"

	"k8s-ovs/cniserver"
	netutils "k8s-ovs/pkg/utils"

	"github.com/golang/glog"

	kclient "k8s.io/kubernetes/pkg/client/unversioned"
	"k8s.io/kubernetes/pkg/kubelet/dockertools"

	cnitypes "github.com/containernetworking/cni/pkg/types"
)

type podHandler interface {
	setup(req *cniserver.PodRequest) (*cnitypes.Result, error)
	update(req *cniserver.PodRequest) error
	teardown(req *cniserver.PodRequest) error
}

type podManager struct {
	// Common stuff used for both live and testing code
	podHandler podHandler
	cniServer  *cniserver.CNIServer
	// Request queue for pod operations incoming from the CNIServer
	requests chan (*cniserver.PodRequest)
	// Tracks pod :: IP address for hostport handling

	dClient dockertools.DockerInterface

	// Live pod setup/teardown stuff not used in testing code
	multitenant bool
	kClient     *kclient.Client
	vnids       *nodeVNIDMap
	ipamConfig  []byte
	mtu         uint32
}

// Creates a new live podManager; used by node code
func newPodManager(multitenant bool, localSubnetCIDR string, netInfo *NetworkInfo, kClient *kclient.Client, dClient dockertools.DockerInterface, vnids *nodeVNIDMap, mtu uint32) (*podManager, error) {
	pm := newDefaultPodManager()
	pm.multitenant = multitenant
	pm.kClient = kClient
	pm.dClient = dClient
	pm.vnids = vnids
	pm.mtu = mtu
	pm.podHandler = pm

	var err error
	pm.ipamConfig, err = getIPAMConfig(netInfo.ClusterNetwork, localSubnetCIDR)
	if err != nil {
		return nil, err
	}

	return pm, nil
}

// Creates a new basic podManager; used by testcases
func newDefaultPodManager() *podManager {
	return &podManager{
		requests: make(chan *cniserver.PodRequest, 20),
	}
}

// Generates a CNI IPAM config from a given node cluster and local subnet that
// CNI 'host-local' IPAM plugin will use to create an IP address lease for the
// container
func getIPAMConfig(clusterNetwork *net.IPNet, localSubnet string) ([]byte, error) {
	nodeNet, err := cnitypes.ParseCIDR(localSubnet)
	if err != nil {
		return nil, fmt.Errorf("error parsing node network '%s': %v", localSubnet, err)
	}

	type hostLocalIPAM struct {
		Type   string           `json:"type"`
		Subnet cnitypes.IPNet   `json:"subnet"`
		Routes []cnitypes.Route `json:"routes"`
	}

	type cniNetworkConfig struct {
		Name string         `json:"name"`
		Type string         `json:"type"`
		IPAM *hostLocalIPAM `json:"ipam"`
	}

	return json.Marshal(&cniNetworkConfig{
		Name: "k8s-ovs",
		Type: "k8s-ovs",
		IPAM: &hostLocalIPAM{
			Type: "host-local",
			Subnet: cnitypes.IPNet{
				IP:   nodeNet.IP,
				Mask: nodeNet.Mask,
			},
			Routes: []cnitypes.Route{
				{
					Dst: net.IPNet{
						IP:   net.IPv4zero,
						Mask: net.IPMask(net.IPv4zero),
					},
					GW: netutils.GenerateDefaultGateway(nodeNet),
				},
				{Dst: *clusterNetwork},
			},
		},
	})
}

// Start the CNI server and start processing requests from it
func (m *podManager) Start(socketPath string) error {
	go m.processCNIRequests()

	m.cniServer = cniserver.NewCNIServer(socketPath)
	return m.cniServer.Start(m.handleCNIRequest)
}

// Returns a key for use with the runningPods map
func getPodKey(request *cniserver.PodRequest) string {
	return fmt.Sprintf("%s/%s", request.PodNamespace, request.PodName)
}

// Add a request to the podManager CNI request queue
func (m *podManager) addRequest(request *cniserver.PodRequest) {
	m.requests <- request
}

// Wait for and return the result of a pod request
func (m *podManager) waitRequest(request *cniserver.PodRequest) *cniserver.PodResult {
	return <-request.Result
}

// Enqueue incoming pod requests from the CNI server, wait on the result,
// and return that result to the CNI client
func (m *podManager) handleCNIRequest(request *cniserver.PodRequest) ([]byte, error) {
	m.addRequest(request)
	result := m.waitRequest(request)
	glog.V(5).Infof("Returning pod network request %v, result %s err %v", request, string(result.Response), result.Err)
	return result.Response, result.Err
}

// Process all CNI requests from the request queue serially.  Our OVS interaction
// and scripts currently cannot run in parallel, and doing so greatly complicates
// setup/teardown logic
func (m *podManager) processCNIRequests() {
	for request := range m.requests {

		result := &cniserver.PodResult{}
		switch request.Command {
		case cniserver.CNI_ADD:
			ipamResult, err := m.podHandler.setup(request)
			if ipamResult != nil {
				result.Response, err = json.Marshal(ipamResult)
			}
			if err != nil {
				result.Err = err
			}
		case cniserver.CNI_UPDATE:
			result.Err = m.podHandler.update(request)
		case cniserver.CNI_DEL:
			result.Err = m.podHandler.teardown(request)
		default:
			result.Err = fmt.Errorf("unhandled CNI request %v", request.Command)
		}
		glog.V(5).Infof("Processed pod network request %v, result %s err %v", request, string(result.Response), result.Err)
		request.Result <- result
	}
	panic("stopped processing CNI pod requests!")
}

redpajama_set_name: RedPajamaGithub


Part No: WP080002 Category: Cartridges for Millipore®. Tags: activated carbon, alternative, anti-scaling, auto-clean, cartridge, depth filtration, elix 20/35/70/100, elix clinical, elix gulfstream, filter, ion exchange, lab water filtration, millipore, pre-treatment cartridge, progard tl1 cl2, progtlcs1, rephile, reverse osmosis, rios 30/50/100/150/200, RR500CPC1.
This PR0GTLCS1 Progard TL1 System Cl2 Auto clean Pretreatment Pak alternative cartridge, has been designed to remove most of the large particles, organic compounds and chlorine from tap water to protect the RO membranes. With a special cleaning media, it will clean and sanitize RO membrane automatically.
Compatible with Millipore® Elix Clinical, Elix Gulfstream, Elix 20/35/70/100, RiOs 30/50/100/150/200 water purification systems.
Technologies employed: Activated carbon, anti-scaling, depth filtration and cleaning media (RO membrane).


We've got a bunch of new posters for you on this fine Friday morning. After the jump, you can find international posters for Rock of Ages, Men in Black 3, Iron Sky, Chernobyl Diaries, Ted, and domestic posters for Ted and Madagascar 3: Europe's Most Wanted.
Hit the jump to check out the posters and for each film's release date and synopsis.
All posters via IMP Awards.
In Men in Black 3, Agents J (Will Smith) and K (Tommy Lee Jones) are back… in time. J has seen some inexplicable things in his 15 years with the Men in Black, but nothing, not even aliens, perplexes him as much as his wry, reticent partner. But when K's life and the fate of the planet are put at stake, Agent J will have to travel back in time to put things right. J discovers that there are secrets to the universe that K never told him — secrets that will reveal themselves as he teams up with the young Agent K (Josh Brolin) to save his partner, the agency, and the future of humankind. The film is directed by Barry Sonnenfeld. The screenplay is written by Etan Cohen, based on the Malibu Comic by Lowell Cunningham. The producers are Walter F. Parkes and Laurie MacDonald, and the executive producers are Steven Spielberg and G. Mac Brown.


text:
U.S./
Title 49. Transportation/
§ 5103
49 U.S.C. § 5103 - U.S. Code - Unannotated Title 49. Transportation § 5103. General regulatory authority
Current as of January 01, 2018 | Updated by FindLaw Staff
Search U.S. Code
(a) Designating material as hazardous. --The Secretary shall designate material (including an explosive, radioactive material, infectious substance, flammable or combustible liquid, solid, or gas, toxic, oxidizing, or corrosive material, and compressed gas) or a group or class of material as hazardous when the Secretary determines that transporting the material in commerce in a particular amount and form may pose an unreasonable risk to health and safety or property.
(b) Regulations for safe transportation.--(1) The Secretary shall prescribe regulations for the safe transportation, including security, of hazardous material in intrastate, interstate, and foreign commerce. The regulations--
(A) apply to a person who--
(i) transports hazardous material in commerce;
(ii) causes hazardous material to be transported in commerce;
(iii) designs, manufactures, fabricates, inspects, marks, maintains, reconditions, repairs, or tests a package, container, or packaging component that is represented, marked, certified, or sold as qualified for use in transporting hazardous material in commerce;
(iv) prepares or accepts hazardous material for transportation in commerce;
(v) is responsible for the safety of transporting hazardous material in commerce;
(vi) certifies compliance with any requirement under this chapter; or
(vii) misrepresents whether such person is engaged in any activity under clause (i) through (vi); and
(B) shall govern safety aspects, including security, of the transportation of hazardous material the Secretary considers appropriate.
[ (C) Repealed. Pub.L. 109-59, Title VII, § 7103(c)(1) , Aug. 10, 2005, 119 Stat. 1894]
(2) A proceeding to prescribe the regulations must be conducted under section 553 of title 5 , including an opportunity for informal oral presentation.
(c) Federally declared disasters and emergencies.--
(1) In general.--The Secretary may by order waive compliance with any part of an applicable standard prescribed under this chapter without prior notice and comment and on terms the Secretary considers appropriate if the Secretary determines that--
(A) it is in the public interest to grant the waiver;
(B) the waiver is not inconsistent with the safety of transporting hazardous materials; and
(C) the waiver is necessary to facilitate the safe movement of hazardous materials into, from, and within an area of a major disaster or emergency that has been declared under the Robert T. Stafford Disaster Relief and Emergency Assistance Act ( 42 U.S.C. 5121 et seq. ).
(2) Period of waiver.--A waiver under this subsection may be issued for a period of not more than 60 days and may be renewed upon application to the Secretary only after notice and an opportunity for a hearing on the waiver. The Secretary shall immediately revoke the waiver if continuation of the waiver would not be consistent with the goals and objectives of this chapter.
(3) Statement of reasons.--The Secretary shall include in any order issued under this section the reasons for granting the waiver.
(d) Consultation. --When prescribing a security regulation or issuing a security order that affects the safety of the transportation of hazardous material, the Secretary of Homeland Security shall consult with the Secretary of Transportation.
(e) Biennial report. --The Secretary of Transportation shall submit to the Committee on Transportation and Infrastructure of the House of Representatives and the Senate Committee on Commerce, Science, and Transportation a biennial report providing information on whether the Secretary has designated as hazardous materials for purposes of chapter 51 of such title all by-products of the methamphetamine-production process that are known by the Secretary to pose an unreasonable risk to health and safety or property when transported in commerce in a particular amount and form.
Cite this article: FindLaw.com - 49 U.S.C. § 5103 - U.S. Code - Unannotated Title 49. Transportation § 5103. General regulatory authority - last updated January 01, 2018 | https://codes.findlaw.com/us/title-49-transportation/49-usc-sect-5103.html
Read this complete 49 U.S.C. § 5103 - U.S. Code - Unannotated Title 49. Transportation § 5103. General regulatory authority on Westlaw
How to Break Up With a Client


text:
<div>
	<video id="videoPlayer" controls="true" style="max-width: 100%; margin: 0 auto;"></video>
</div>
<script src="http://192.168.33.10/js/dashjs/dist/dash.all.js"></script>
<script>
(function(){
	var url = "http://e8mk2e7v154v5.elementalclouddemo.com/out/i/3.mpd";
	var context = new Dash.di.DashContext();
	var player = new MediaPlayer(context);
	player.startup();
	player.attachView(document.querySelector("#videoPlayer"));
	player.attachSource(url);
})();
</script>


text:
Agenda about the City Council meeting of August 20, 2013
This Agenda is about the Hickory City Council meeting that will take place on the date listed above. City council meetings are held on the first and third Tuesdays of each Month in the Council Chambers of the Julian Whitener building.
City Website has changed - Here is a link to the City of Hickory Document Center
All materials and maps for this meeting are provide at this link:
Hickory City Council Agenda - August 20, 2013
Invocation by Rev. Bob Shoffner, Retired Lutheran Pastor
A. Presentation of the Carolina Cycle Challenge
B. Business Well Crafted Award: Lenoir Rhyne University
C. Announcement of a Community Gift to the City
A. Call for Public Hearing - For the Consideration of Voluntary Satellite Annexation of Property Located at 2633 Springs Road NE, Hickory, Containing 1.675 Acres (Authorize Public Hearing for September 3, 2013)
B. Transfer of Cemetery Deed from Robert T. Link and wife, Heather Dawn Link and Travis W. McLeod and wife, Sonya McLeod to Robert George Hadley and wife, Elaine Verbyla Hadley in Oakwood Cemetery
C. Approval to Declare as Surplus Vehicle Emergency Equipment and Donate to the Cooksville Volunteer Fire Department. - Hickory Police Department is removing four police vehicles from the vehicle fleet that have reached the end of useful life. The emergency equipment including light bars and sirens have been removed from these vehicles and are no longer of use to the department. Staff request approval to donate four light bars, four light bar control units, four siren box control units and four siren speaker units to the Cooksville Volunteer Fire Department which is a nonprofit organization. Public Notice advertised on August 14, 2013 in a newspaper having general circulation in the Hickory Area.
D. Request from Hickory Police Department to Award Police Badge and Service Weapon to Retiring Deputy Chief Clyde Deal. - By authority of NC General Statute §20-187.2, City Council may award the service weapon and police badge to retiring Deputy Chief Clyde Deal upon his retirement from Hickory Police Department on September 1, 2013 after completing 30 years of qualifying service with Hickory Police Department. Upon approval from City Council, the police badge and service weapon will be declared surplus and removed from the City's fixed asset inventory.
E. Approval of a Resolution and Lease Agreement Between the City of Hickory and the State of North Carolina Forest Services for Hanger W2 Located on the West Ramp of the Hickory Regional Airport. - The North Carolina Forest Service desires to lease the entire hangar/office space of
Hangar W2 for a period of ten years with the mutual option to renew said lease for one additional five year term. The rate will be $6,416.67 per month for the first year, every year thereafter during the term of said lease the rate will increase $2,000 per year to include any five year renewal term. Public Notice advertised on August 10, 2013 in a newspaper having general circulation in the Hickory Area.
A. Report of Mayor Wright's Travel to attend a Meeting with the North Carolina League of Municipalities on August 5, 2013 (mileage - $195.49).
1. Voluntary Satellite Annexation of Property Located at 1076 Fox Chase Drive containing .42 acres. - Crystal Kuhar has submitted a petition for the voluntary satellite annexation of .42 acres of property located in the Fox Chase Subdivision off Sandy Ford Road in Catawba County. The petitioner requested annexation in order to connect to the City of Hickory's sanitary sewer system. The property currently serves as Ms. Kuhar's primary residence. This public hearing was advertised on August 9, 2013 in a newspaper having general circulation in the Hickory area.
1. Vacant Building Revitalization and Demolition Grant Agreement for ZLoop Knitting Mill, LLC. - The applicant ZLoop Knitting Mill, LLC, plans to spend $83,600 to demolish the former Walton Knitting Mill building located at 838 14th Street NE, Hickory. The grant program can reimburse up to $20,000 or 35 percent of eligible project costs, whichever is less for demolition projects. This project would be eligible for a grant in the amount of $20,000. After demolition the applicant will use the site as a parking lot for the adjacent development. The Business Development Committee reviewed the application and voted to recommend approval at their August 6th meeting.
2. Appointments to Boards and Commissions


Looks completely void of content to me. Or is it empty because no-one has left a comment recently? That's possible, I reckon.
Me too. We might as well exchange thoughts on work via PMs or emails. This break-down eclipses user/author interaction.


text:
Analysis: Let Slam rules be — 5 sets; no 'breaker in Paris
Stadium workers watch Argentina's Diego Schwartzman celebrate his win as the duration of the match, five hours and eight minutes, is shown in the quarterfinal match of the French Open tennis tournament against Austria's Dominic Thiem at the Roland Garros stadium in Paris, France, Tuesday, Oct. 6, 2020.
Michel Euler
As it is, Rafael Nadal would be a big favorite to win his French Open semifinal, of course. He is, after all, a 12-time champion and a combined 24-0 in that round and finals at Roland Garros; he's 9-1 against Friday's opponent, Diego Schwartzman.
There's also this working in Nadal's favor: He is coming off a three-set quarterfinal; Schwartzman toiled for five sets across 5 hours, 8 minutes in his previous match.
That's an advantage Nadal earned, in part, by being more efficient. He deserves any edge it gives him — just like Schwartzman had an edge in his quarterfinal against Dominic Thiem, who had gone five sets in the fourth round. That's merely one reason that any discussion of switching from best-of-five-set matches to best-of-three for men at Grand Slam tournaments is misguided.
Others: The current format allows for more plot twists, more comebacks, more suspense, more drama; it makes major championships distinct from lesser events; it rewards superior stamina and focus; it fosters fascinating and — sometimes, though not always — memorable matches.
When Stefanos Tsitsipas, the 22-year-old from Greece who faces No. 1 Novak Djokovic in the semifinals Friday, talked about skipping school to watch Roland Garros on TV as a kid, the first match that sprang to mind was a 6-hour, 33-minute win for Fabrice Santoro over Arnaud Clement in 2004 that ended 16-14 in the fifth.
"I watched some epic thrillers, five-set matches," Tsitsipas said.
Each of the past four Slam men's finals went
Let Slam rules be — 5 sets; no 'breaker in Paris
© Provided by Associated Press
Stadium workers watch Argentina's Diego Schwartzman celebrate his win as the duration of the match, five hours and eight minutes, is shown in the quarterfinal match of the French Open tennis tournament against Austria's Dominic Thiem at the Roland Garros stadium in Paris, France, Tuesday, Oct. 6, 2020. (AP Photo/Michel Euler)
Argentina's Diego Schwartzman catches his breath after a rally in the fourth set of the quarterfinal match of the French Open tennis tournament against Austria's Dominic Thiem at the Roland Garros stadium in Paris, France, Tuesday, Oct. 6, 2020. (AP Photo/Alessandra Tarantino)
When Stefanos Tsitsipas, the 22-year-old from Greece who faces No. 1 Novak Djokovic in the semifinals Friday, talked about skipping school to
Each of the past four Slam men's finals went five sets and were better for it; two ended in tiebreakers. That can't happen this Sunday, and that's OK.
That's why it's also misguided to think the French Open should join the other tennis major tournaments in adopting final-set tiebreakers.
"The subject has been discussed," the French tennis federation told The


Weather "Mesh" Tarps « Erickson Manufacturing Ltd.
The mesh design allows the air to flow through and works great for covering loads that need containment but not water repellency. The flapping we see with most tarps is drastically reduced with the mesh design.


text:
Four types of human platelet lysate, including one virally inactivated by solvent-detergent, can be used to propagate Wharton jelly mesenchymal stromal cells
Ming Sheng Chen, Tsung Jen Wang, Hsiu Chen Lin, Burnouf Thierry
臺北醫學大學附設醫院
眼科學科
小兒學科
臺北醫學大學生醫器材研究中心
生醫材料暨組織工程研究所
國際生醫工程博士學位學程
細胞治療與再生醫學研究中心
細胞治療與再生醫學國際博士學位學程
臺北醫學大學神經醫學研究中心
There is accumulating experimental evidence that human platelet lysate (HPL) made from platelet concentrates can replace fetal bovine serum (FBS) as a xeno-free clinical-grade supplement of growth media to expand mesenchymal stromal cells (MSCs). However, uncertainties exist in regard to impacts that various manufacturing methods of HPL can exert on the expansion and differentiation capacity of MSCs. In particular, there is a need to evaluate the possibility of implementing virus-inactivation treatment during HPL production to ensure optimal safety of industrial HPL pools. Expired human platelet concentrates from four different donors were pooled and subjected to freeze-thaw cycles (-80/+37 °C), followed or not by serum-conversion by calcium chloride, heat-treatment at 56 °C for 30 min, or solvent-detergent (S/D) virus inactivation. The concentrations of total proteins, growth factors and fibrinogen, and the chemical compositions of the HPLs were characterized. The impact of HPL supplementation on the cell morphology, doubling time, immunophenotype and trilineage differentiation capacity of Wharton jelly MSCs (WJMSCs) were compared over five passages, using FBS as a control and normalizing the protein content. Data showed that WJMSCs expanded equally well, exhibited a typical fibroblast morphology, had short doubling times, maintained their immunophenotypes, and differentiated into chondrocyte, osteocyte, and adipocyte lineages in all HPL-supplemented media, all of which were more effective than FBS. In conclusion, we found minimal detectable impact of the HPL manufacturing process, including S/D virus inactivation, on the suitability of expanding WJMSCs in vitro.
New Biotechnology
https://doi.org/10.1016/j.nbt.2018.11.003
已發佈 - 三月 25 2019
10.1016/j.nbt.2018.11.003
指紋 深入研究「Four types of human platelet lysate, including one virally inactivated by solvent-detergent, can be used to propagate Wharton jelly mesenchymal stromal cells」主題。共同形成了獨特的指紋。
Wharton Jelly Medicine & Life Sciences
Detergents Engineering & Materials Science
Platelets Engineering & Materials Science
Mesenchymal Stem Cells Medicine & Life Sciences
Blood Platelets Medicine & Life Sciences
Virus Inactivation Medicine & Life Sciences
Viruses Engineering & Materials Science
Chen, M. S., Wang, T. J., Lin, H. C., & Thierry, B. (2019). Four types of human platelet lysate, including one virally inactivated by solvent-detergent, can be used to propagate Wharton jelly mesenchymal stromal cells. New Biotechnology, 49, 151-160. https://doi.org/10.1016/j.nbt.2018.11.003
Four types of human platelet lysate, including one virally inactivated by solvent-detergent, can be used to propagate Wharton jelly mesenchymal stromal cells. / Chen, Ming Sheng; Wang, Tsung Jen; Lin, Hsiu Chen; Thierry, Burnouf.
於: New Biotechnology, 卷 49, 25.03.2019, p. 151-160.
Chen, MS, Wang, TJ, Lin, HC & Thierry, B 2019, 'Four types of human platelet lysate, including one virally inactivated by solvent-detergent, can be used to propagate Wharton jelly mesenchymal stromal cells', New Biotechnology, 卷 49, 頁 151-160. https://doi.org/10.1016/j.nbt.2018.11.003
Chen MS, Wang TJ, Lin HC, Thierry B. Four types of human platelet lysate, including one virally inactivated by solvent-detergent, can be used to propagate Wharton jelly mesenchymal stromal cells. New Biotechnology. 2019 3月 25;49:151-160. https://doi.org/10.1016/j.nbt.2018.11.003
Chen, Ming Sheng ; Wang, Tsung Jen ; Lin, Hsiu Chen ; Thierry, Burnouf. / Four types of human platelet lysate, including one virally inactivated by solvent-detergent, can be used to propagate Wharton jelly mesenchymal stromal cells. 於: New Biotechnology. 2019 ; 卷 49. 頁 151-160.
@article{c814165cd2f745669695616add95f696,
title = "Four types of human platelet lysate, including one virally inactivated by solvent-detergent, can be used to propagate Wharton jelly mesenchymal stromal cells",
abstract = "There is accumulating experimental evidence that human platelet lysate (HPL) made from platelet concentrates can replace fetal bovine serum (FBS) as a xeno-free clinical-grade supplement of growth media to expand mesenchymal stromal cells (MSCs). However, uncertainties exist in regard to impacts that various manufacturing methods of HPL can exert on the expansion and differentiation capacity of MSCs. In particular, there is a need to evaluate the possibility of implementing virus-inactivation treatment during HPL production to ensure optimal safety of industrial HPL pools. Expired human platelet concentrates from four different donors were pooled and subjected to freeze-thaw cycles (-80/+37 °C), followed or not by serum-conversion by calcium chloride, heat-treatment at 56 °C for 30 min, or solvent-detergent (S/D) virus inactivation. The concentrations of total proteins, growth factors and fibrinogen, and the chemical compositions of the HPLs were characterized. The impact of HPL supplementation on the cell morphology, doubling time, immunophenotype and trilineage differentiation capacity of Wharton jelly MSCs (WJMSCs) were compared over five passages, using FBS as a control and normalizing the protein content. Data showed that WJMSCs expanded equally well, exhibited a typical fibroblast morphology, had short doubling times, maintained their immunophenotypes, and differentiated into chondrocyte, osteocyte, and adipocyte lineages in all HPL-supplemented media, all of which were more effective than FBS. In conclusion, we found minimal detectable impact of the HPL manufacturing process, including S/D virus inactivation, on the suitability of expanding WJMSCs in vitro.",
keywords = "FBS, Human platelet lysates, Propagation, Solvent/detergent, Wharton jelly stem cells",
author = "Chen, {Ming Sheng} and Wang, {Tsung Jen} and Lin, {Hsiu Chen} and Burnouf Thierry",
note = "Funding Information: We thank the Taipei Blood Center (Taiwan Blood Service Foundation, Guandu, Taiwan) for supplying the platelet concentrates. This study was financially supported in part by a grant from Taipei Medical University Hospital ( 104TMU-TMUH-07 ). The sponsor played no role in the data collection, analysis, interpretation; manuscript writing, or decision to submit the article for publication. ",
doi = "10.1016/j.nbt.2018.11.003",
journal = "New Biotechnology",
T1 - Four types of human platelet lysate, including one virally inactivated by solvent-detergent, can be used to propagate Wharton jelly mesenchymal stromal cells
AU - Chen, Ming Sheng
AU - Wang, Tsung Jen
AU - Lin, Hsiu Chen
AU - Thierry, Burnouf
N1 - Funding Information: We thank the Taipei Blood Center (Taiwan Blood Service Foundation, Guandu, Taiwan) for supplying the platelet concentrates. This study was financially supported in part by a grant from Taipei Medical University Hospital ( 104TMU-TMUH-07 ). The sponsor played no role in the data collection, analysis, interpretation; manuscript writing, or decision to submit the article for publication.
N2 - There is accumulating experimental evidence that human platelet lysate (HPL) made from platelet concentrates can replace fetal bovine serum (FBS) as a xeno-free clinical-grade supplement of growth media to expand mesenchymal stromal cells (MSCs). However, uncertainties exist in regard to impacts that various manufacturing methods of HPL can exert on the expansion and differentiation capacity of MSCs. In particular, there is a need to evaluate the possibility of implementing virus-inactivation treatment during HPL production to ensure optimal safety of industrial HPL pools. Expired human platelet concentrates from four different donors were pooled and subjected to freeze-thaw cycles (-80/+37 °C), followed or not by serum-conversion by calcium chloride, heat-treatment at 56 °C for 30 min, or solvent-detergent (S/D) virus inactivation. The concentrations of total proteins, growth factors and fibrinogen, and the chemical compositions of the HPLs were characterized. The impact of HPL supplementation on the cell morphology, doubling time, immunophenotype and trilineage differentiation capacity of Wharton jelly MSCs (WJMSCs) were compared over five passages, using FBS as a control and normalizing the protein content. Data showed that WJMSCs expanded equally well, exhibited a typical fibroblast morphology, had short doubling times, maintained their immunophenotypes, and differentiated into chondrocyte, osteocyte, and adipocyte lineages in all HPL-supplemented media, all of which were more effective than FBS. In conclusion, we found minimal detectable impact of the HPL manufacturing process, including S/D virus inactivation, on the suitability of expanding WJMSCs in vitro.
AB - There is accumulating experimental evidence that human platelet lysate (HPL) made from platelet concentrates can replace fetal bovine serum (FBS) as a xeno-free clinical-grade supplement of growth media to expand mesenchymal stromal cells (MSCs). However, uncertainties exist in regard to impacts that various manufacturing methods of HPL can exert on the expansion and differentiation capacity of MSCs. In particular, there is a need to evaluate the possibility of implementing virus-inactivation treatment during HPL production to ensure optimal safety of industrial HPL pools. Expired human platelet concentrates from four different donors were pooled and subjected to freeze-thaw cycles (-80/+37 °C), followed or not by serum-conversion by calcium chloride, heat-treatment at 56 °C for 30 min, or solvent-detergent (S/D) virus inactivation. The concentrations of total proteins, growth factors and fibrinogen, and the chemical compositions of the HPLs were characterized. The impact of HPL supplementation on the cell morphology, doubling time, immunophenotype and trilineage differentiation capacity of Wharton jelly MSCs (WJMSCs) were compared over five passages, using FBS as a control and normalizing the protein content. Data showed that WJMSCs expanded equally well, exhibited a typical fibroblast morphology, had short doubling times, maintained their immunophenotypes, and differentiated into chondrocyte, osteocyte, and adipocyte lineages in all HPL-supplemented media, all of which were more effective than FBS. In conclusion, we found minimal detectable impact of the HPL manufacturing process, including S/D virus inactivation, on the suitability of expanding WJMSCs in vitro.
KW - FBS
KW - Human platelet lysates
KW - Propagation
KW - Solvent/detergent
KW - Wharton jelly stem cells
U2 - 10.1016/j.nbt.2018.11.003
DO - 10.1016/j.nbt.2018.11.003
JO - New Biotechnology
JF - New Biotechnology


text:
Ultra-sensitive polymer detects explosive devices (IEDs)
Will Dichtel, assistant professor of chemistry, and graduate student Deepti Gopalakrishnan hold samples of their polymer that uses fluorescence to detect the explosive RDX.
Credit: Jason Koski/University Photography
Date:10 June 2013 Tags:Cornell University, IEds, new polymer, William Dichtel
By Anne Ju
A chemical that's often the key ingredient in improvised explosive devices (IEDs) can be quickly and safely detected in trace amounts by a new polymer created by a team of chemists at Cornell University in the US. The polymer, which potentially could be used in low-cost, handheld explosive detectors and could supplement or replace bomb-sniffing dogs, was invented in the lab of William Dichtel, assistant professor of chemistry and chemical biology. The work was published online in the Journal of the American Chemical Society.
RDX, short for research department explosive, is an explosive material common in military and industrial applications that is also a favourite of bomb-making terrorists. It requires a detonator to explode, but when detonated, it's more powerful than TNT. What's more, RDX's vapour pressure is 1 000 times lower than that of TNT, making it almost impossible to detect without direct contact with a concentrator, such as the swabs used by airport security staff.
Dichtel and graduate student Deepti Gopalakrishnan made a polymer that uses fluorescence to quickly and accurately ascertain whether RDX is present on a surface or in the air. Says Dichtel: "One of the goals is to make detectors that can detect not just explosives on someone's hands, but in the cloud around them – much like the dust cloud surrounding Charlie Brown's friend, Pigpen. "If someone had an IED in their bag, it would be nice to not have to open it."
The researchers' work builds on a previously established technology that uses "fluorescence quenching" as the basis for detecting TNT; in the presence of the explosive, the polymer's fluorescence shuts off. The polymer has a random, cross-linked structure that allows it to absorb light and transport the resulting energy throughout its structure. After a certain period of time, the polymer releases this energy as light, a process known as fluorescence. If the energy encounters a molecule of explosive as it travels through the polymer, it can be converted into heat instead of light, which causes the polymer to stop glowing. This design allows the polymer fluorescence to sense extremely small amounts of the explosive of interest, enabling identification of IEDs or people who have recently handled them.
The experiments also involved testing a host of other chemicals, such as those found in lipstick and sunscreen, to rule out false positives.
Source: Cornell University