text:
WO2002072002A2 - Method for preparing stabilised reaction mixtures, which are totally or partially dried, comprising at least one enzyme, reaction mixtures and kits containing said mixtures - Google Patents
Method for preparing stabilised reaction mixtures, which are totally or partially dried, comprising at least one enzyme, reaction mixtures and kits containing said mixtures Download PDF
WO2002072002A2
WO2002072002A2 PCT/ES2002/000109 ES0200109W WO02072002A2 WO 2002072002 A2 WO2002072002 A2 WO 2002072002A2 ES 0200109 W ES0200109 W ES 0200109W WO 02072002 A2 WO02072002 A2 WO 02072002A2
WIPO (PCT)
PCT/ES2002/000109
WO2002072002A3 (en
Pedro Manuel Franco De Sarabia Rosado
Gemma LIMONES LÓPEZ
Antonio MADEJÓN SEIZ
María Dolores MARÍN ALBERDI
Biotools Biotechnological & Medical Laboratories, S.A.
2001-03-12 Priority to ESP0100569 priority Critical
2001-03-12 Priority to ES200100569A priority patent/ES2180416B1/en
2002-03-11 Application filed by Biotools Biotechnological & Medical Laboratories, S.A. filed Critical Biotools Biotechnological & Medical Laboratories, S.A.
2002-09-19 Publication of WO2002072002A2 publication Critical patent/WO2002072002A2/en
C—CHEMISTRY; METALLURGY
C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
Y10S435/00—Chemistry: molecular biology and microbiology
Y10S435/975—Kit
The invention relates to a method consisting in bringing into contact, in one container, (a) an aqueous solution of a reaction mixture comprising at least one enzyme and (b) an aqueous solution of a stabilising mixture comprising (i) at least one agent to protect against drying, (ii) at least one inhibitor of the condensation reaction between carbonyl or carboxyl groups and amine or phosphate groups, and (iii) at least one inert polymer capable of generating a mesh structure that impedes the mobility of the dried reagents. The inventive method also consists in removing all or part of the water contained in the resulting aqueous solution. Said method is suitable for carrying out enzymatic reactions, for example, amplifying, sequencing and characterising nucleic acids, performing hybridisation tests and for the restriction analysis.
PROCEDURE FOR PREPARING STABILIZED REACTION MIXTURES, totally or partially dried, comprising at least one enzyme, KITS AND REACTION MIXTURES CONTAINING
The invention relates to the preparation of mixtures stabilized, totally or partially dried reaction, comprising at least one enzyme, by adding a stabilizing mixture to a solution containing the reaction mixture, and subsequent removal of the all or part of the water present in the resulting solution, also the resulting reaction mixtures and ITS comprising said reaction mixtures.
Deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) are long responsible for storing and transmitting genetic information acromoléculas linear. They consist of monomeric units called nucleotides, each of which consists of a nitrogenous base, purine or pyrimidine, a simple sugar (deoxyribose in the case of DNA and ribose in the case of RNA), and an inorganic phosphate. In a nucleic acid, nucleotides are linked together by phosphodiester bonds through adjacent nucleotide sugar. The nucleobases are covalently linked to the sugar-phosphate backbone. Each nucleic acid mainly has four different nucleobases, two pyrimidines and two purines. Purine bases are the same for both DNA and RNA, both containing adenine (A) and guanine (G). With respect to the pyrimidine bases, nucleic acids containing both cytosine (C), the ina ti (T) specific DNA and uracil (U) RNA specific. The sequence of these nucleobases determines the genetic information carried by nucleic acids as well as their three dimensional conformation. While the RNA is generally found in nature as a single polynucleotide chain, DNA is normally present as a double polynucleotide chain both chains have antiparallel associated by hydrogen bonds formed between a purine base and a complementary pyrimidine each.
The nucleic acid amplification is the most widely used for the precise identification of a specific nucleic acid, consisting of the exponential multiplication of the initial amount of a specific or characteristic nucleic acid segment tool. This amplified segment can be used in downstream applications such as cloning and restriction analysis. The nucleic acid sequencing is the determination of the nucleotide sequence of a nucleic acid fragment determined. Amplification and sequencing of nucleic acids and analysis of restriction fragment technique of identifying or characterizing a nucleic acid based on differential sizes caused by endonuclease enzymes called restriction enzymes, are techniques widely used in biomedical research, in medical diagnostics and veterinary contagious and hereditary diseases in human and animal genetic analysis and in the analysis of food, environmental monitoring, and forensics and forensic analysis, among other possible uses. Several methods for the synthesis and amplification in vi tro nucleic acid. The most known and used is the polymerase chain reaction, commonly known by the acronym PCR (Polymerase Chain Reaction)
(Saiki et al., Science, 230, 1350-1354 (1985), Mullis et al., US patents US 4,683,195, US 4,683,202 and US 4,800,159). In its simplest form, PCR is a cyclic process of DNA amplification which one or more specific DNA sequences contained in a nucleic acid or mixture of nucleic acids is amplified exponentially using two oligonucleotide primers specifically bind, by base complementarity, two opposing DNA problem previously denatured by heat regions. Each of the DNA strands problem which has joined its corresponding primer can be copied to the form of complementary DNA by the action of a DNA polymerase activity, using the oligonucleotide as a primer for the polymerization reaction. Repeated successive cycles of this process an exponential enrichment of the DNA fragment problem between the points of hybridization of the oligonucleotides. Using thermostable DNA polymerases allows successive cycles of denaturation, annealing of oligonucleotide DNA substrate and elongating the chain, without adding new polymerase activity in each cycle. In case of analyzing the presence of DNA with a relative abundance this process is sufficient for identification. However, it is common to analyze samples in which the abundance of DNA problem is below the limits of a simple PCR. For analysis of these samples it has been developed the technique of nested amplification PCR or nested-. In this system two coupled amplification processes are performed, the second of which uses as the substrate the amplified product of a first PCR. To increase the specificity of the oligonucleotides used process in the second amplification reaction are different from those used in the first and hybridized with internal areas of the product of the first amplification. Since the amplification reaction uses DNA polymerases activities requiring as substrate DNA molecules, the direct analysis of RNA molecules requires prior reverse transcription step (RT or reverse transcription) by which a DNA copy is synthesized (cDNA ) complementary to the RNA problem. The cDNA thus obtained can be used as substrate an amplification reaction (Mocharla et al, Gene. 93: 271-275 (1990)). The amplification system used may be a simple reaction (RT-PCR) or nested amplification (RT-nested PCR), depending on the relative abundance of the original RNA substrate. The development of DNA polymerase activities modified reverse transcriptase activity allows the performance of RT-amplification process using a single enzyme activity in high stringency conditions.
Methods of nucleic acid amplification cyclic described above using thermostable polymerase enzymes obtained from various thermophilic microorganisms such as Thermus aquaticus (Kaledin et al, Biokhimiya 45, 644-651 (1980). Chien et al., J. Bacteriol . 127: 1550- 1557 (1976); US patent 4,889,818), and Thermus thermophil us (Ruttimann et al J. Biochem 149: 41-46 (1985...)), among others. Several of these enzymes thermostable DNA polymerases, for example, from T. aqua ticus (Jones et al, Nucleic Acids Research. 17: 8387-8388 (1989)), like DNA polymerases esófilas some such enzymes as DNA polymerase I Escherichia coli (..... Karkas et al, Proc Natl Acad Sci USA 70: 3834-3838 (1973); Leob et al, Nature New Biol 242:.. 66-69 (1973)) have a dual activity enzymatic DNA polymerase and reverse transcriptase, depending on the cofactor present in the reaction mixture, so that in the presence of magnesium ion has DNA polymerase activity while in the presence of manganese ion has reverse transcriptase activity.
Thermostable enzymes, unlike mesophilic proteins do not denature heat is more, they require higher temperatures to perform its activity. This is mainly due to changes in its amino acid sequence, moving to the overall folding or tertiary structure. These modifications are not drastic, but thermostability arises from the sum of small enthalpic interactions and entropic type that contribute synergistically to obtain a more rigid and heat resistant tertiary structure. These modifications include amino acid changes, leading to a more rigid tertiary structure (Menéndez-Arias and Argos, J.Mol.Biol, 206. 397-406 (1989)), or the removal of waste are sensitive to degradation by deamidation (Asn, Gln) or oxidation (Met, Cys), especially in the most sensitive areas of the protein, such as loops or loops
(Watanabe et al, J. Biol .Chem., 266:. 24287-24294 (1991)). The formation of hydrogen bonds and formation of large networks including, between the amino acids of the protein as water molecules in the medium (Pace et al, FASEB J., 10:. 75-83 (1996)) is another changes that lead to thermostability, as the formation of salt bridges between charged residues on the surface, or as a way to neutralize the polar residues remaining in the hydrophobic interior of the protein (Yip et al, Structure, 3:. 1147 -1158 (1995)). Other modifications of this type are the formation of secondary structures, especially -hélices by stabilizing its internal dipole (Rentier-Delrue et al, J.Mol.Biol. 229:. 85- 93)) and the formation of bridges hydrogen (Warren and Petsko, Prot.Eng., 8: 905-913 (1995)), as well as increasing the packaging in the hydrophobic interior, that is, make more compact protein (Matthews, FASEB J. 10:35 - 41 (1996)). Based on the foregoing, it is evident that the preservation of tertiary structure of thermostable enzymes, and therefore their activity and functionality passes by preserving the integrity of protein interactions with water molecules that surround, even in greater extent than mesophilic enzymes not thermostable.
It is well known that products such as gelatin, bovine serum albumin (BSA), ammonium sulfate and Thesit, among others, stabilize polymerase enzymes and dNTPs, and nonionic surfactants such as NP40 and Tween 20 improve reaction of nucleic acid amplification (Saiki et al, Science, 239:. 487-491 (1988)). Restriction enzymes are endonucleases enzymes that break the phosphodiester bonds within the DNA double chain. One type of these restriction enzymes, called type II, have the property of recognizing a particular nucleotide sequence of the DNA, causing the breaking of the bonds linking the nucleotides together within that determined sequence, consequently cutting one DNA fragments in accurate and reproducible, creating what is called a restriction map. The Type II enzymes are widely used in various techniques of molecular, such as cloning, identification of specific sequences and DNA fragments, and analysis of restriction maps, among others (Molecular Cloning Biology: a Laboratory Manual, J. Sambrook et al , 2nd Ed. 1989).
Very frequently, the activity of enzymes and other biological macromolecules, including antigens and antibodies, depends mainly on their three dimensional conformation, called tertiary structure, so that if this three-dimensional shape is modified by any factor, biological activity or functionality macromolecule may decrease or even disappear. Water forms a protective shell around the biological macromolecules, stabilizing the tertiary structure of the macromolecules by hydrophobic / hydrophilic interactions. These interactions also block the reactive chemical groups which can be found on the surface of the macromolecules. By removing this protective casing aqueous what happens for example in any drying process, distortions may occur in the tertiary structure of the macromolecules, thereby being modified, and also reactive chemical groups (amino groups, phosphate, carboxyl, etc.) are free to react with each other or with other reactive groups of other nearby macromolecules, may contribute to the loss of the original tertiary structure and even forming aggregates between various identical or different each other macromolecules, which also generally implies the decrease or even loss of biological activity or functionality of macromolecules, like changes in their tertiary structures. These reactions between peripheral reactive chemical groups can occur even once conveniently desiccated macromolecules due to the plasticity of the media in which they are, why can observe a decrease in biological activity or functionality of the dried macromolecules pass of the time. It is therefore of great importance replacing the aqueous envelope surrounding macromolecules by protective agents to replace effectively water molecules in their mission of keeping the tertiary structures of the macromolecules and mission stabilization and protection the surface reactive groups.
The usual method of storage and transportation of DNA polymerases and enzymes restriction aforementioned and other enzymes used in molecular biology and for the aforementioned uses, comprising freezing at -20 ° C, stabilizing them to withstand these low temperatures by mainly adding glycerol to the aqueous form. This method preserves the enzyme activity for several months with little loss of activity as long as the storage temperature does not rise above -20 ° C so it is crucial to preserve the cold chain, important losses of activity or inactivating enzymes if this cold chain is interrupted for several hours. Even using this method of transport and storage by freezing at -20 ° C, is not advisable to ship and conservation of all reaction components in a single container, vial or tube, since they undesirable chemical or biochemical reactions between the various components could cause their inactivation or generating artifacts that could interfere or mask the interpretation of experimental results. From the above it follows that it would be of great economic advantage to have a system that would enable the conservation and transport of said macromolecules at room temperature, can be avoided in this way the necessary preservation of the cold chain, especially if this conservation system permitted dispatching to the market in a single container all elements (enzymes, cofactors, additives, etc.) required for carrying out the desired enzyme reaction. In the amplification of nucleic acids by the techniques described above, each of the components involved in the reaction, ie, DNA polymerase enzyme, reaction buffer containing enhancing additives reaction or stabilizers, magnesium chloride, or manganese chloride in case of RT, the oligonucleotides used as primers of the reaction, the deoxyribonucleotides (dATP, dCTP, dGTP and dTTP), and the sample containing the nucleic acid to be amplified, are separately preserved by freezing as is explained above, and must be mixed prior to carrying out the reaction, being necessary to add and mix small amounts (microliters) of each of them, with frequent errors in dosing and pipetting of each of the above reagents, which ends up generating uncertainty about the reproducibility of the results obtained by applying of these techniques, uncertainty of particular concern in the case of human diagnosis. This variability in the possibility of error in pipetting of the various reagents to be added to the amplification reaction also affects the sensitivity of the technique, which generates a new uncertainty about the application of these techniques in the human disease diagnosis, and especially in determining levels of infection and gene expression levels.
. Furthermore, during pipetting and adding to the reaction mixture of the test sample, aerosols frequently cause cross contamination between samples to be analyzed (Kwok, S. et al, Nature, 339 occur: 237238 (1989), generating false positive results, of great importance also in the case of human diagnosis.
Various techniques have been described and used for the preservation by drying of biological macromolecules, and its use and applicability are conditioned by their ability to preserve the functional characteristics of the product on which it is applied.
Lyophilization is the principal of these techniques described preservation by drying, and a method in which water removal is achieved by freezing the wet product and subsequent sublimation of ice at low pressure. Sublimation is the process by which a solid evaporates without passing through the liquid state, so that the term low pressure indicated above refers to a value of vapor pressure below the triple point of water vapor pressure in the water coexists in the solid, liquid and vapor states. To perform lyophilization should proceed to complete freezing of the solution or lyophilised product at a temperature and speed depend on the material to be lyophilized. The equipment necessary for freeze drying are expensive, and the lyophilisation process is relatively slow, so very often these economic factors discourage their use. Other drying systems are well known, such as drying on a fluidized bed, drying at room temperature and atmospheric pressure, drying at ambient temperature and pressure decreased, drying at a high temperature and atmospheric pressure, drying at high temperature and reduced pressure. The choice of drying method to use depends on its degree of efficiency, of the more or less aggressive versus composition to preserve by drying, and economic factors.
Various solutions have been proposed to preserve the integrity of biological macromolecules during the drying process as well as for stabilizing and preserving the functionality of the dried during preservation and storage.
Clegg et al. described in Cryobiology 19: 306-316 (1982) The protective effect of glycerol and trehalose on the cellular response to desiccation.
Crowe et al. described in Cryobiology 20: 346-356 (1983), Archives Biochem. Biophysies 232: 400-497 (1984) and Biochimia & Biophysica Acta 769: 141-150 (1984), the action of various carbohydrates for the stabilization of cell membranes, indicating the protective effect significantly greater than the non-reducing disaccharide known as trehalose ( α-D-glucopyranosyl-α-D-glucopyranoside) in drying the cellular organelles.
Carpenter et al. (Cryobiology 24: 455-464 (1987)) indicate that the disaccharides maltose, sucrose, lactose and trehalose increase the stability of the activity of a purified preparation of enzyme phosphofructokinase after desiccation.
The European patent application EP 140489 from Wako Pure Chemical Industries, discloses a method for protecting an immunoactive protein (antibody) on a conveyor (eg a glass bead) against drying at ambient temperature by immersion in a sugar solution optionally together with a protein such as bovine serum albumin. a considerable number of sugars (ribose, glucose, fructose, mannose, galactose, maltose, saccharose (sucrose), lactose and other oligosaccharides and polysaccharides), being preferred based on their greater protective effect lactose, sucrose it mentioned and dextrin solutions. US patent 4,891,319, granted to Quadrant Bioresources Limited, describes the use of trehalose as a protective agent against drying of proteins, antibodies, and other biological macromeléculas.
Tooru O. et al. described in Chemical Abstracts 95: 517 (1981) The protective effect of sugars and sugar alkoxylic against denaturation and drying muscular fibers in fish.
Patent Application EP 91258 discloses a method of stabilization of tumor necrosis factor (TNF) by storage or lyophilization of the purified TNF protein in the presence of a stabilizing protein such as bovine serum albumin or gelatin.
The patent application WO 91/18091, of Quadrant Holdings Cambridge Limited, describes a method of preserving biological substances in a desiccated state using non-reducing glycosides of polyhydroxy compounds from (preferably sorbitol or mannitol), such as maltitol, lactitol and both palatinitol isomers, and a non-reducing oligosaccharide such as raffinose, stachyose and melezitose.
Igaki et al. in J. JPN. Diabetes Soc, 34: 403-407 (1991) indicate L-lysine as an inhibitor of the condensation reactions between carbonyl and amino groups on the periphery of biological macromolecules.
5,955,448 US patent, granted to Quadrant Holdings Cambridge Limited, claims a method of stabilizing samples and biological macromolecules, including restriction endonuclease enzymes, dried by adding nonreducing carbohydrate and an inhibitor of the condensation reactions between amino groups and peripheral carbonyl. It is further known stabilization β- recombinant interferon using a stabilizing agent such as a detergent or glycerol, may also contain other proteins, sucrose, trehalose, and other polyhydroxy carbohydrate derivatives as additional stabilizing agents, especially dextrose.
Other references to methods of drying and preservation against desiccation of biological macromolecules can be found in MJ Pikal, BioPharm 3: 18- 20, 22-23, 26-27 (1990), Carpenter et al, Cryobiology 25:. 459 -470 (1988), B. Roser, Biopharm 4: 47-53 (1991), Colaco et al, Bio / Technol. 10: 1007-1011 (1992), and Carpenter et al, Cryobiology 25:. 244-255 (1988). Moreover, US 5,861,251 the patent, granted to Bioneer Corporation, claims a process of preparing a reactive ready to use dried by lyophilization for DNA amplification by adding glucose as preservatives or sorbitol to a reaction mixture contains all the components necessary to carry out the reaction, ie a DNA polymerase enzyme thermostable, a reaction buffer containing all the components necessary to carry out the reaction, and deoxynucleotide triphosphates or dideoxynucleotide triphosphates required as substrates for the reaction polymerization, plus a water soluble dye, not citing the addition of probes or oligonucleotide primers to the reagent ready for use. 5,614,387 US patent, assigned to Gen-Probe Incorporated, describes a reagent ready to use dried by lyophilization for RNA amplification by adding as preservatives of a non-reducing disaccharide, preferably trehalose or sucrose, and / or polyvinylpyrrolidone (PVP ), to a reaction mixture containing all reagents necessary to perform nucleic acid amplification, including an RNA polymerase and / or reverse transcriptase, not mentioning in the examples no thermostable enzyme together with a reaction buffer containing all necessary to perform the reaction components, and deoxynucleotide triphosphates or dideoxynucleotide triphosphates required as substrates of the polymerization reaction, plus a water soluble dye. 5,935,834 US patent, assigned to Asahi Kasei Kogyo Kabushiki Kaisha, claims a reagent ready for use obtainable by drying and preservation in the presence of trehalose and containing all the elements necessary for reverse transcription of RNA from a virus.
Although there have been proposed various solutions to preserve the integrity of biological macromolecules during the drying process as well as for stabilizing and preserving the functionality of the dried during preservation and storage, there remains a need to develop alternative solutions that increase the potential means to satisfy such purposes. The present invention provides a solution to this need.
The invention provides a process for preparing a stabilized reaction mixture and totally or partially dried, said mixture comprising at least one enzyme, hereinafter method of the invention, comprising:
a) contacting in a single container:
i) an aqueous solution of a reaction mixture comprising at least one enzyme; Y
ii) an aqueous solution of a stabilizing mixture composed of
- at least one protective agent against drying;
- at least one inhibitor of the condensation reaction between carbonyl or carboxyl groups and amine or phosphate groups; Y
- at least one inert polymer capable of generating a mesh-shaped structure preventing the mobility of the dried reagents;
to obtain an aqueous solution comprising said reaction mixture together with said stabilizing mixture; Y
b) removing all or part of the water contained in said aqueous solution obtained in step a), until obtaining a totally or partially dried mixture comprising said enzyme and said stabilizing mixture and has a moisture content not exceeding 30%, to obtain a mixture of stabilized, totally or partially dried reaction, comprising at least one enzyme.
The reaction mixture comprises one or more enzymes involved in one or more enzymatic reactions with all or part of the necessary reagents for conducting enzymatic reactions in which said enzymes intervene. In a particular embodiment, the reaction mixture contains all reagents required for conducting the reaction or reactions in which the enzyme (s) (s) present in the reaction mixture involved, mixed in suitable quantities in said container, as a reaction tube or well of a multiwell plate, wherein subsequently after rehydrating and adding the substrate or problem sample is carried out the enzymatic reaction to be performed.
The enzyme or enzymes present in the reaction mixture can be any enzyme. In a particular embodiment, said enzyme is selected from the group consisting of enzymes of nucleic acid amplification, both thermostable and thermolabile nucleic acids, both RNA and DNA, restriction enzymes, enzymes involved in amplification reactions, sequencing or characterization nucleic acid, and mixtures thereof. Therefore, in a particular embodiment, the reaction mixture comprises an enzyme selected from amplification of nucleic acids, restriction enzymes, enzymes involved in amplification reactions, sequencing and characterization of nucleic acids, and mixtures thereof, with all necessary for performing the reactions involved the above enzymes, and including cofactors, enzyme substrates and other additives which enhance enzymatic reactions and may also optionally include probes and oligonucleotide primers, labeled or without reagents mark, for carrying out a specific amplification, for example, detecting a determined pathogen or genetic mutation in a sample. The aqueous solution of the reaction mixture can be prepared outside the container and then added thereto as such or can be formed directly in the container by adding and mixing the various components of the reaction mixture in the container itself. The stabilizer mixture is composed of (i) at least one protective agent against drying, (ii) at least one inhibitor of the condensation reaction between carbonyl or carboxyl groups and amine or phosphate groups; and (iii) at least one inert polymer, when dried, generates a mesh-shaped structure preventing the mobility of the dried reagents.
The protective agent against drying has the main task of stabilizing the tertiary structure of enzymes and optionally present nucleotides in the reaction mixture during the drying process, in this purpose substituting the water molecules in aqueous solution form protective wrap that helps maintain the three-dimensional structure of macromolecules also blocking reactions that may occur between the reactive chemical groups that may exist on the surface of macromolecules, which also have a stabilizing effect in preserving long within the dried mixtures. The protective agent against drying may be a suitable non-reducing carbohydrate, in particular a di- or trisaccharide nonreducing, or a mixture of such compounds. In a particular embodiment, the protective agent against drying is selected from the nonreducing disaccharides palatinitol (6- D-glucopyranosyl-mannitol) and trehalose, trisaccharides raffinose and melezitose not reducers, and mixtures thereof. Other non-reducing carbohydrates such as sucrose (sucrose), has been effective in drying enzymes, but not in the drying both enzymes and oligonucleotides.
Have been tested various non-reducing compounds, polyalcohols group, as protective agents against drying and stabilizing of the dried mixtures during storage, such as sorbitol and glycerol. Both relatively effective in their independent use as protective agents for enzymes during drying and subsequent storage, especially sorbitol. In the process of joint desiccation reaction mixtures containing enzymes and oligonucleotides together with the stabilizer mixture containing non-reducing carbohydrates, inhibitors of the condensation reactions and inert polymers, it is appreciated that while glycerol has a beneficial protective effect in all cases in conjunction with the components of the stabilizer mixture, especially in conjunction with non-reducing carbohydrates melezitose, palatinitol, trehalose and sucrose, sorbitol does not exhibit any beneficial effect, but on the contrary, reaches inhibit the protective effect of the foregoing . The use of sorbitol as a protective agent against desiccation and stabilizer during storage of the reaction mixtures is effective when such reaction mixtures are not present oligonucleotides exhibiting no protective or stabilizing action when such mixtures oligonucleotide chains are present. Therefore, in addition to one or more non-reducing carbohydrates, the stabilizing mixture may optionally contain glycerol as a protective agent against drying. The inhibitor of the condensation reactions have the purpose of inhibiting the condensation reactions which may occur between the reactive carboxyl, carbonyl, amino and phosphate found in the surface of the macromolecules present in the reaction mixture to be dried, so that must be present in an amount sufficient to exert this inhibitory effect. Reaction inhibitors used may be competitive or noncompetitive. Among competitive inhibitors, various amino acids have been proven the most effective, stressing the plant, arginine and tryptophan, and among them, lysine. Among non-competitive, betaine and aminoguanidine derivatives have proven to be the most effective. The choice of noncompetitive inhibitor depends on the non-reducing carbohydrate used, so that in the presence of the most effective raffinose noncompetitive inhibitor is betaine, whereas in the presence of other carbohydrates are aminoguanidine derivatives most effective. Therefore, in a particular embodiment, the stabilizing mixture containing an inhibitor of the condensation reaction between carbonyl or carboxyl groups and amine or phosphate groups selected from the group consisting of lysine, arginine, tryptophan, betaine, aminoguanidine derivatives, and mixtures thereof.
The inert polymer capable of generating a structure in a mesh preventing the mobility of the dried reagents to desiccate the aqueous solution is located, is primarily mission improve the storage stability of the reaction mixture dried to generate a mesh preventing the mobility of the various reagents composing the reaction mixture, so are immobilized to a greater or lesser extent in the cells that form the polymer and, consequently, these reagents may not approach each other, thus preventing the chemical reaction their surface reactive groups. The polymer should, on the one hand not react with any of the reagents comprising the blend. reaction, and on the other hand, you must create a grid fine enough to trap moldable macromolecules in its mesh individualized without distorting its tertiary or quaternary structure when dried. In a particular embodiment, said inert polymer is selected from the group consisting of polyvinylpyrrolidone (PVP), polyethylene glycol (PEG) of varying degrees of polymerization, dextran, starch, the compound called FICOLL (synthesized from sucrose nonionic polymer, Pharmacia ), glycogen, gum arabic and mixtures thereof. Generally, the glycogen and acacia gum are inert polymers have been more effective in its protective function. Another object of this inert polymer is used as a cryoprotective macromolecules present in the reaction mixture to drying by lyophilization, to generate an amorphous mesh that prevents the crystallization of water during the initial characteristic freezing that drying process. The amount of inert polymer to be added to the mixture of drying should be sufficient to ensure the generation of a mesh sufficiently thick to prevent the mobility of macromolecules, without then interfere with the enzymatic reaction to occur after rehydration dried reaction mixture.
The joint action of the three components of the stabilizing mixture (protective agent, inhibitor condensation reactions and inert polymer) causes the aforementioned reaction mixtures are completely functional after drying and prolonged storage. The addition of one or two of said components, without the presence of the other two or remaining component generates reaction mixtures or are not active after drying or their activity disappears a few days after drying, presenting therefore very reduced stability during storage. Non-reducing carbohydrates (protecting agents), in conjunction or not with the inhibitor of the condensation reactions can effectively protect against drying and stabilize during storage the enzymes and the reaction mixtures containing all reagents necessary for embodiment except probes or oligo-nucleotide primers, but not to the reaction mixtures containing these oligonucleotides. The same happens with the nonreducing sugar alcohol sorbitol, which is shown separately as a good protective and stabilizing the reaction mixture containing all necessary reagents except the oligonucleotides, but stops exercising this protective effect when the reaction mixture oligonucleotides are present. The inhibitor of the condensation reactions, used exclusively in the drying or in the presence of inert polymer, is capable of protecting the reaction mixture without oligonucleotides during the drying process being a drop in the yield of the reaction when the mixture reaction containing oligonucleotides.
The aqueous solution of the stabilizer mixture can be prepared outside the container and subsequently added to it which may either be formed directly in the container by adding and mixing the various components of the stabilizing mixture in the container itself. After the mixture in the container the aqueous solution of the reaction mixture with the stabilizing mixture comprising aqueous reaction mixture comprising at least one enzyme, together with said stabilizing mixture is formed. Then all or part of the water contained in the resulting aqueous solution of the reaction mixture with the stabilizing mixture is removed until a totally or partially dried mixture comprising the enzyme or enzymes together with said stabilizing mixture and has a moisture content exceeding 30% to obtain a stabilized mixture, totally or partially dried reaction, comprising at least one enzyme.
Removal of all or part of the water present in the aqueous solution obtained in the container after mixing the reaction mixture with the stabilizing mixture can be accomplished by any conventional drying method, including, for example, lyophilization, drying in fluidized bed , drying at room temperature and atmospheric pressure, drying at ambient temperature and decreased pressure, drying at high temperature and atmospheric pressure, drying at high temperature and reduced pressure. The preferred drying method is drying at a temperature between 15 ° C and 60 ° C, and reduced pressure below atmospheric. Other methods, such as those previously mentioned, can be applied in drying, although their higher cost or less or more aggressive efficiency versus the components of the reaction mixture to be dried dissuade use.
The reaction mixtures stabilized, dried, provided by this invention have a moisture content not exceeding 30%, preferably equal to or less than 20%.
The mixtures completely dried reaction, that is, with a presence of residual water equal to or less than 1%, tend to have stability during less than those containing a higher percentage of water storage appreciated in completely desiccated a significant decrease in reaction yields after rehydrating and adding the reaction substrate. Moreover, in the dried mixtures containing percentages comprised residual water between 1% and 20%, macromolecules exhibit mobility that may cause chemical reactions or undesirable enzyme, despite the presence of the inert polymer impede mobility complete, so although they exhibit stability several weeks at room temperature (25 ° C), should preferably be stored at temperatures between 4 ° C and 10 ° C to ensure correct functionality over time. The degree of drying mainly depends on economic factors (cost of the process, time required to reach a certain degree of drying, etc.) and the relationship between the degree of drying and the stability of the reaction mixture. Therefore, in a particular embodiment, the percentage of relative humidity remaining in the mixture of stabilized and dried reaction is between 1% and 20%.
By the process of the invention mixtures stabilized, totally or partially dried reaction in a single tube or well of a plate, contain all the necessary elements, (eg, enzymes, cofactors, substrates, oligonucleotide reaction primers and other are obtained additives enhancing or modulating the enzymatic reaction) so that can be carried out the desired after rehydrating and adding the substrate or problem sample enzymatic reaction, thereby avoiding the need to preserve the cold chain in the transport and handling of these dried reaction mixtures, simplifying necessary for the enzymatic reaction, for example, nucleic acid amplification, not being necessary to preserve and adding each reaction component separately previniéndose cross-contamination and pipetting errors normal handling, increasing consequently, repeatability and fia ity of analysis. The stabilized reaction mixtures, totally or partially dried, maintain their activity without significant loss thereof, after transportation and storage at room temperature. The invention also provides a mixture of stabilized reaction and totally or partially dried, with a moisture content exceeding 30%, preferably between 1% and 20%, comprising at least one enzyme and a stabilizer mixture as the previously defined comprising (i) at least one protective agent against drying, (ii) at least one inhibitor of the condensation reaction between carbonyl or carboxyl groups and amine or phosphate groups, and (iii) at least an inert polymer capable of generating a mesh-shaped structure preventing the mobility of the dried reagents.
In a particular embodiment, said mixture of stabilized and totally or partially dried reaction contains an enzyme selected from the group consisting of enzymes nucleic acid amplification, restriction enzymes, enzymes involved in amplification reactions, sequencing or characterization of nucleic acids, and mixtures thereof, together with all or part of the necessary reagents for carrying the enzymatic reactions involving these enzymes, preferably with all such reagents, including cofactors, enzyme substrates and other additives that improve the enzymatic reactions and may also optionally include probes and oligo-nucleotide primers, labeled or unlabeled, for carrying out a specific amplification, for example, detecting a determined pathogen or genetic mutation in a sample. Thus stabilized mixtures, totally or partially dried reaction in a single tube or well of a plate obtained containing all the necessary elements
(Ie, enzymes, cofactors, substrates, oligonucleotide reaction primers and other additives enhancing or modulating the enzymatic reaction) that can be carried out the desired reaction after rehydrating and adding the substrate or problem sample.
In a particular embodiment, the invention provides a stabilized mixture, totally or partially dried, useful for amplifying one or more specific sequences of one or more nucleic acids, RNA or DNA reaction present in a sample. Said reaction mixture comprises a DNA polymerase enzyme or enzyme reverse transcriptase, thermostable or not, deoxynucleotide triphosphates, labeled or unlabeled by any of the known methods, all necessary cofactors for enzymatic activity and other additives enhancing or modulating said enzymatic activity. The mixture of stabilized and dried reaction may also contain oligonucleotide reaction primers, labeled or unlabeled by any of the known methods, required for the specific amplification of the target nucleotide sequence, and / or oligonucleotide probes, labeled or unlabeled by any of the known methods, necessary for performing a hybridization assay, as well as any additive or coadjuvant of the hybridization reaction. This mixture stabilized and dried reaction is in a single container, such as a reaction tube or well of a multiwell plate, and to perform the desired reaction rehydrating the reaction mixture is only necessary by adding distilled water and the sample. Preferably, the drying method is the previously explained and contains the described stabilizing mixture.
In another particular embodiment, the invention provides a mixture of stabilized and totally or partially dried reaction useful for sequencing nucleic acids, wherein the mixture of stabilized and dried reaction described above is added also dideoxyribonucleotides (ddNTPs), eliminating the water soluble dye. Preferably, the drying method is the previously explained and contains the described stabilizing mixture.
In another particular embodiment, the invention provides a mixture of stabilized and totally or partially dried reaction useful for carrying out restriction analysis on a multiwell plate, so that the test sample a reaction mixture is deposited on the small cup containing dessicated It contains a determined restriction enzyme with which it is desired to treat the sample as well as all the cofactors and additives necessary for carrying out the restriction analysis, subsequently proceeding to the incubation of the sample with the mixture of reaction rehydrated. Preferably, the drying method is the previously explained and contains the described stabilizing mixture. In another particular embodiment, the invention provides a reaction mixture stabilized and totally or partially dried for carrying out hybridizations in multiwell plate so that the sample to hybridize is deposited on the container containing dried the oligonucleotide probes, labeled or unlabeled by any of the known methods, necessary for performing the hybridization assay, as well as any additive or coadjuvant of the hybridization reaction, subsequently proceeding to conducting the hybridization reaction. Preferably, the drying method is the previously explained and contains the described stabilizing mixture.
The stabilized reaction mixtures, totally or partially dried, provided by this invention, are of the "ready for use" (ready-to-use) and have numerous applications, among which are the diagnosis of disease, determining the DNA sequence, or restriction analysis of nucleic acids, according to the methods described below and are illustrated in the examples accompanying this description.
Stabilized mixtures, totally or partially dried reaction provided by this invention also can be used as reaction system "hot-start." Various studies conducted by the inventors have revealed that the amplification reaction tubes containing said stabilized reaction mixtures, totally or partially dried, runs with equal efficiency whether resuspended or not the contents of the tube prior to effecting PCR (see Example I). This could mean that the reactants of said mixture of stabilized, totally or partially dried reaction is effectively released during the early stages of the reaction, for example, during incubation at elevated temperature. This would operate at such mixtures stabilized wholly or parcilalmente descadas reaction provided by this invention as hot-start system. This aspect is very important for several reasons since: a) from a practical point of view facilitates handling system avoiding resuspension of the mixture [indeed, for volumes larger sample (20 or more tubes) individual resuspension of each one of them involves a lot of work, to the point that may be longer to make a conventional mix]; whereas, on the contrary, not having to make resuspension drastically shortens the work to be done; b) from a scientific point of view the hot- start systems are ideal in amplification systems since they increase the specificity of the system; in this sense, the system provided by this invention allow work comfortably with the tubes on ice and ensure the release of reactive hot.
The invention also provides a kit comprising a mixture of stabilized and dried reaction fully or partially, with a moisture content exceeding 30%, preferably between 1% and 20%, provided by this invention. In a particular embodiment, said kit is suitable for amplification of one or more specific sequences of one or more nucleic acids, RNA or DNA kit, present in a sample or for performing a hybridization assay of nucleic acids, or for nucleic acid sequencing, or for carrying out restriction analysis on a multiwell plate, or for carrying out hybridizations in multiwell plate. The kit of the invention contains, besides the mixture of stabilized, totally or partially dried reaction, comprising at least one enzyme involved in an enzymatic reaction, all or part, preferably all of the reagents, factors, additives and / or oligonucleotide sequences necessary for performing the enzymatic reaction involved.
The following examples illustrate further the present invention and should not be construed as limiting the scope of the invention.
EXAMPLE I Drying the reaction mixture for DNA amplification The thermostable DNA polymerase enzyme used in this and the following examples, unless indicated otherwise, is a recombinant DNA polymerase from Thermus thermophilus expressed in Escherichia coli, property of Biotools B & M Labs SA, Spain, and purified using a non-chromatographic method developed by the same company (BIOTOOLS DNA Polymerase). After purification, the enzyme was stored at -20 ° C in a storage buffer containing 30 mM Tris HCl, pH 8, 25 mM glucose, 25 mM KC1, 0.5 mM PMSF, 0.25% Tween 20 and NP40 0 , 25%. A reaction buffer containing Tris HCl, pH 8, 750 mM (NH 4) 2 S0 4 200 mM, Tween 20 0, 1% and 20 mM MgCl 2 was prepared.
To each tube 0.2 ml reaction used in the experiment was added 1 microliter of said enzyme DNA polymerase (1 U / .mu.l) retained in its storage buffer, 5 microliters of the reaction buffer, and 1 microliter of a solution in equimolar containing the four deoxyribonucleotides (dNTPs) involved in the amplification reaction of DNA (dATP, dCTP, dGTP and dTTP). Several tubes as described above, and each of them was added the appropriate amounts of each of the stabilizing mixtures encompassed in Table 1. The tubes thus prepared were dried in a centrifugal evaporator Eppendorf 5301 were prepared at temperatures between 10 ° C and 60 ° C, for a time comprised between 30 and 120 minutes. The temperatures and times mentioned above vary according to the final volume of dry mixture.
After drying, the tubes were conserved at the temperatures and times indicated in Table 1. At the times indicated in Table 1, was assayed its activity by amplification reaction specific cytochrome b region, a size 359 base pairs (bp), adding 43 microliters of bi-distilled water, one microliter of each reaction primers
1-1: 5'- CCATCCATCTCAGCATGATGAAA-3 '(SEQ ID NO:.. 1); and 1-2: 5'-GCCCCTCAGAATGATATTTGTCCTCA-3 '(SEQ ID NO:.. 2), and 500 ng of DNA to a final volume of 50 microliters.
In half of the tubes the gelled contents of the vial was resuspended by pipetting (5 times) in 50 microliters of water, and DNA primers added. In the other half of the tubes, the contents of the vial gelled not reslurried. Incubation was performed at 94 ° C for 2 minutes
(Although this incubation cycle is not strictly necessary, but optional), and then 35 cycles of denaturation (94 ° C, 10 seconds), annealing (55 ° C, 30 seconds), and extension were performed (72 ° C, 40 seconds), using a thermocycler Eppendorf Mastercycler. In parallel, and to check the evolution of the activity of the dried tubes, DNA samples were amplified using a fresh mixture, under the same conditions of amplification.
The result of the amplification reaction was analyzed in all cases 2% agarose gel (w / v), and found in all cases in which the amplification was positive, a single 359 bp band. The activity of the dried reaction mixtures and fresh mixtures was measured by densitometry of the band resulting from the amplification, using an image analyzer TDI Gelprinter using the Gelsuper computer program also developed by TDI. The activity results of each dried tube are semi-quantitatively expressed in relation to the results obtained with the fresh mixtures. It was considered that the dried mixtures had: maximum activity (+++ in Table 1) when the intensity of the band was 90% ± 10% from the fresh mixture; optimum activity (++ in Table 1) when the activity ranged between 90% and 50%, low activity (+ in Table 1) when it was less than 50%, and absence of activity (- in Table 1) when the result of the amplification reaction was negative.
The obtained activity results can were seen in the column "activity Example I" in Table 1. In conclusion, although several stabilizing mixtures can be considered suitable by having conserved a good activity after drying and subsequently storing the reaction mixture, they are those containing melezitose or palatinitol, in conjunction with lysine and glycogen or acacia gum, or raffinose with betaine and glycogen which conserve a higher activity. In all cases it was observed that the activity obtained in the tubes in which the gel content of the vial was resuspended before performing PCR, and the activity obtained in the tubes was resuspended not like. This seems to show that there is an effective release of the reagents contained in the gel mixture during incubation at high temperature, which would act as a hot-start system.
EXAMPLE II Drying of the reaction mixture for reverse transcription RNA and subsequent amplification of cDNA
The transcriptase enzyme thermostable reverse used in this example was recombinant DNA polymerase from Thermus thermophil us transcriptase activity in improved reverse respect to the enzyme indicated in Example I, I expressed in Escherichia coli, property of Biotools B & M Labs SA (Spain), and purified using a non-chromatographic method developed by the same company (RETR0T00LS cDNA / DNA Polymerase). After purification, the enzyme was stored at - 20 ° C in a storage buffer containing Tris HC1, 30 mM, pH 8, 25 mM glucose, 0.5 mM PMSF, 0.25% Tween 20 and 0.25% NP40. Reaction buffer was prepared for reverse transcription containing 75 mM Tris HCl, pH 8, (NH 4) 2 S0 4 200 mM, Tween 20 0.01%, MnCl 2 1.5 mM, and 0.125 mM of each dNTP (dATP, dGTP, dTTP, dCTP).
Each reaction tube 0.2 milliliters used in this experiment 1.5 microliters of this enzyme (5 U / .mu.l) kept in storage buffer, 4 microliters of reaction buffer for reverse transcription, and 1 microliter was added a solution containing in equimolar dATP, dCTP, dGTP and dTTP. In another 0.2 ml tube 6 microliters of reaction buffer consisting of 75 mM Tris HCl, pH 8 are added (NH 4) 2 S0 4 20 mM, 0.01% Tween 20, 0.75 mM EGTA and 2 mM MgCl 2. Several tubes as described above, and each was added one of the stabilizing mixtures encompassed in Table 1. The tubes thus prepared were dried in a centrifugal evaporator Eppendorf 5301, at temperatures between prepared 10 ° C and 60 ° C, for a period of time between 30 and 120 minutes. The temperatures and times mentioned above vary according to the stabilizing mixture used. After drying, the tubes were conserved at the temperatures and times indicated in Table 1.
The activity of the reaction mixtures, and of the fresh mixtures desiccated not was assayed by amplification reaction of a specific region of the gene mouse CD8, of a size of 1122 bp, rehydrating the dried mixture with 15 microliters of bi-distilled water adding 1.25 microliters of the reaction primers: II-l: 5'-CAAGGATGCTCTTGGCTCTT-3 '(SEQ ID NO:.. 3); and II-2: 5'-GTGGTAGCAGATGAGAGTGA-3 'SEQ. ID. N °: 4), and 100 ng of mRNA extracted from mouse to a final volume of 20 microliters. The reverse transcription mixture thus reconstituted incubated at 60 ° C for 30 minutes to synthesize cDNA. Then, the dried tubes with DNA amplification mixture were rehydrated with 30 ul of H 2 0. The entire volume of the rehydrated reaction buffer was added to the tubes in which had undergone the reverse transcription reaction. Then 40 cycles of denaturation (94 ° C, 45 seconds), annealing (55 ° C, 30 seconds) and extension (72 ° C, 1 minute) were performed using a thermocycler Eppendorf Mastercycler. The experiment was designed so that the tubes used for reverse transcription reaction and the tubes used in the reaction of subsequent DNA amplification had dried and stored under the same conditions. Amplification products were analyzed by agarose gel electrophoresis 1% (w / v), being in all cases in which the amplification was positive, a single band of 1122 bp. The intensity of the bands amplification of all samples was measured by densitometry of the band resulting from the amplification, using an image analyzer TDI Gelprinter using the Gelsuper computer program also developed by TDI. The activity results of each dried tube are semi-quantitatively expressed in relation to the results obtained with the fresh mixtures. It was considered that the dried mixtures had: maximum activdad (+++ in Table 1) when the intensity of the band was 90% ± 10% with respect to the fresh mixture, optimum activity (++ in Table 1) when the activity ranged between 90% and 50%, low activity (+ in Table 1) when it was less than 50%, and absence of activity (- in Table 1) when the result of the amplification reaction was negative.
The obtained activity results can be seen in the "example II activity" column of Table 1. In conclusion, although several stabilizing mixtures can be considered suitable by having conserved a good activity after drying and subsequently storing the reaction mixture, again They are those containing melezitose or palatinitol, in conjunction with lysine and glycogen or acacia gum, or raffinose with betaine and glycogen which conserve a higher activity.
EXAMPLE III Drying restriction enzyme in conjunction with its cutting buffer were placed in eppendorf tubes 1.5 ml used in this experiment 1 microliter of HindIII restriction enzyme (1 U / .mu.l) marketed by MBI Fermentas, Lithuania, with 1 microliter of 10X cutting buffer supplied by the manufacturer with the enzyme. Several tubes as described above were prepared and each of them was added the appropriate volume of each of the different stabilizing mixtures listed in Table 1. The tubes thus prepared were dried on a centrifugal evaporator Eppendorf model 5301, at temperatures between 10 ° C and 60 ° C, for a period of time between 30 and 120 minutes. The temperatures and times mentioned above vary according to the stabilizing mixture used.
After drying, the tubes were conserved at the temperatures indicated in Table 1. Their activity was assayed once elapsed the time period corresponding to that indicated in Table 1 by digestion at 37 ° C for 2 hours 0.3 microgram DNA (150 ng / .mu.l) of phage lambda, after rehydrating with 8 microliters of bidistilled water. electrophoresis the result of the digestion reaction was carried agarose gel 1% (w / v), in parallel with a digestion with a fresh enzyme preparation performed in the same conditions of time and temperature. The activity of the dried enzyme was divided into three categories:
A) optimum activity ( "digestion" in Table 1) when the complete DNA digestion was obtained with a pattern identical to that obtained by digesting with restriction enzyme fresh; B), ( "partial" in Table 1) average activity when a pattern different from that obtained with the fresh enzyme restriction, due to the existence of partial digestions was obtained; Y
C) inactive ( "no cut" in Table 1) when the complete absence of digestion was observed.
The results of the comparison of the activity of the restriction enzyme desiccated with its cutting buffer for activity having a digestion same characteristics produced by a fresh enzyme not dried can be seen in the "activity example III" column Table 1. in conclusion, those are again stabilizing mixtures containing melezitose or palatinitol, in conjunction with lysine and glycogen or acacia gum, or raffinose with betaine and glycogen those which generate complete digestions times longer storage while other mixtures tested generate partial digestions cut or not.
Other restriction enzymes such as Mbo, Bgl II, Rsa I, Ava I, Ava II and Acc I were also tested with identical results.
EXAMPLE IV Desiccation tube and on a multiwell plate reaction mixture including oligonucleotide primers for the specific amplification of a particular DNA sequence
A reaction mixture was prepared containing in addition to all the components specified in Example I, the reaction primers described in Spanish patent of invention n ° P200100568 and which serve for identification in a single reaction multiplexed amplification mechanism of seminested reaction in one step, of the four species causing Plasmodi um developing malaria in humans, as well as human DNA, which serves as a positive control reaction. In this method human DNA samples extracted from whole blood by standard methods below.
The reaction of simultaneous amplification of DNA from each of the four Plasmodium species and control human DNA amplification is performed in a tube including separate reaction (0.2 ml or 0.5 ml) 25 .mu.l of a mixture reaction with the following composition: 75 mM Trizma base, 20 mM ammonium sulfate, 0.01% Tween 20, 5 mM MgCl 2, 0.5 mM dATP, 0.5 mM dCTP, 0.5 mM dGTP, dTTP 0, 5 mM primer Hl (SEQ ID NO:.. 5) 0.04 uM, primer H2 (SEQ ID NO:.. 6) 0.04 uM, Pl primer (SEQ ID NO:.. 7) 2 , 25 uM, primer P2 (SEQ ID NO:.. 8) .01 uM, primer F (SEQ ID NO:.. 9) 0.6 mM M primer (SEQ ID NO:.. 10) 0.15 uM, 0 primer (SEQ ID NO:.. 11) 0.375 uM, V primer (SEQ ID NO:.. 12) 0.15 uM and 2 units of DNA polymerase (Biotools DNA polymerase).
Several tubes as described above, and each was added one of the stabilizing mixtures encompassed in Table 1, at different concentrations of each of the elements of the mixtures were prepared. The tubes thus prepared were dried in a centrifugal evaporator Eppendorf 5301, at temperatures between 10 ° C and 60 ° C, for a period of time between 30 and 120 minutes. The temperatures and times mentioned above vary according to the stabilizing mixture used.
After drying, the tubes were conserved at the temperatures indicated in Table 1. After the conservation periods indicated in said Table 1, their activity was assayed after rehydrating the dried mixture with 20 microliters of bi-distilled water and 50 ng of DNA from of patients infected with different species of Plasmodi um, to a final volume of 25 microliters. Also, as an activity reference, tubes with fresh non-dried mixture in all experiments included. Amplification cycles used include an initial heating cycle at 85 ° C for 3 minutes followed by a denaturation step at 94 ° C for 7 minutes. Then the round of amplification itself consisting of 40 repeated cycles each of which consists of a denaturation step at 94 ° C for 45 seconds, an annealing step at 62 ° C for 45 seconds and a step is performed elongation at 72 ° C for 1 minute. After completion of 40 cycles of amplification is a final elongation step at 72 ° C for 10 minutes. The amplification reaction was performed in a thermocycler Eppendorf Mastercycler. An electrophoresis result of the amplification reaction in agarose gel 2% (w / v) was performed and was found in all cases in which the amplification was positive, 2 bands of 395 bp and 231 bp corresponding to the products of amplification of P. falciparum and human DNA respectively. The activity of the dried reaction mixture was measured by densitometry of the band resulting from the amplification, using an image analyzer TDI Gelprinter using the Gelsuper computer program also developed by TDI. The activity results of each dried tube are semi-quantitatively expressed in relation to the results obtained with the fresh mixtures. It was considered that the dried mixtures had: maximum activity (+++ in Table 1) when the intensity of the band was 90% ± 10% with respect to the fresh mixture, optimum activity (++ in Table 1) when the activity ranged between 90% and 50%, low activity (+ in Table 1) when it was less than 50%, and absence of activity (- in Table 1) when the result of the amplification reaction was negative.
The obtained activity results can be seen in the "activity example IV" column of Table 1. In conclusion, although several stabilizing mixtures can be considered suitable by having conserved a good activity after drying and subsequently storing the reaction mixture, are those containing melezitose or palatinitol, in conjunction with lysine and glycogen or acacia gum, or raffinose with betaine and glycogen which conserve a higher activity.
An identical analytical process was followed by depositing on each well of a multiwell polystyrene plate Nunc brand the previously mentioned reaction mixture containing the oligonucleotide primers, drying the plate by introducing it in a desiccator and creating a vacuum by a pump. a plate for each time point and analyzed subsequently temperature was prepared. Also they were prepared for each experiment fresh wells undried reference activity. The amplification conditions and results analysis were identical to those described above. The results of measuring the activity of the dried sample fully coincide with those obtained for the case of the previously mentioned tubes.
EXAMPLE V
Drying tube and on a multiwell plate of a reaction mixture including oligonucleotide primers for the specific amplification of a specific RNA sequence a reaction mixture was prepared containing in addition to all the components specified in Example II, the reaction primers described in the Spanish patent application P200100567 of invention n ° and serve for the generic detection of enterovirus genome by a coupled reverse transcription system followed by an amplification reaction semi-nested in a single tube or pocilio.
To perform the reverse transcription reaction are prepared by each reaction 20 microliters of a mixture containing 75 mM Tris HCl, (NH 4) 2 S0 4 20 mM, Tween 20 0.01%, MnCl 2 1.5 mM, 0.125 mM of each dNTP (dATP, dCTP, dGTP, dTTP) and 10 picomoles of primer EV1M: 5'- ACCCAAAGTAGTCGGTTCCGC-3 '(SEQ ID NO:.. 13) and 7.5 units of DNA polymerase enzyme indicated in Example II. To carry out the DNA amplification reaction are prepared by each reaction 30 microliters of a mixture containing 75 mM Tris HCl, pH 8, (NH 4) 2 S0 4 20 mM, Tween 20 0.01%, EGTA 0, 75 mM, 2 mM MgCl 2, 10 pmol primer EV2P: 5'-CAAGCATTCTGTTTCCCC-3 '(SEQ ID NO:.. 14) and 0.5 picomoles of primer EV1P: 5'-CGGTACCTTTGTRCGCCTGTT-3' (SEQ. ID NO. 15).
Several tubes as described above, and each was added different volumes of each of the stabilizing mixtures are shown in Table 1. The tubes thus prepared were dried in a centrifugal evaporator Eppendorf 5301, they were prepared temperatures between 10 ° C and 60 ° C, for a period of time between 30 and 120 minutes. The temperatures and times mentioned above vary according to the stabilizing mixture used.
After drying, the tubes were conserved at the temperatures and times indicated in Table 1. Their activity was assayed in the time also indicated in Table 1, after rehydrating the mixture dried reverse transcription with 15 microliters of bi-distilled water, adding 100 ng of RNA to a final volume of 20 microliters. 4 cycles of 48 ° C / 5 minutes and 60 ° C / 15 minutes were performed. It was resuspended in 30 microliters of bidistilled water 0.2 ml tube which was used in the subsequent reaction DNA amplification, which contains the same stabilizer mixture as its homologue used for reverse transcription, and its volume was added on 0.2 ml tube containing the product of reverse transcription. 35 cycles of denaturation (94 ° C, 1 minute), annealing (48 ° C, 1 minute) and extension (72 ° C, 1 minute) were performed using a thermocycler Eppendorf Mastercycler. An electrophoresis result of the amplification reaction in 2% agarose gel (w / v) was performed and was found in all cases that the amplification was positive, showing two bands of 390 bp and 489 bp. In all experiments included fresh tubes mixture were used as an activity reference. The activity of fresh and dried mixtures of reaction was measured by densitometry of the band resulting from the amplification, using an image analyzer TDI Gelprinter using the Gelsuper computer program also developed by TDI. It was considered that the dried mixtures had: maximum activity (+++ in Table 1) when the intensity of the band was 90% ± 10% with respect to the fresh mixture, optimum activity (++ in Table 1) when the activity ranged between 90% and 50%, low activity (+ in Table 1) when it was less than 50%, and absence of activity (- in Table 1) when the result of the amplification reaction was negative. Activity results obtained can be seen in the column "example V activity" in Table 1. In conclusion, although several stabilizing mixtures can be considered suitable by having conserved a good activity after drying and subsequently storing the reaction mixture, They are those containing melezitose or palatinitol, in conjunction with lysine and glycogen or acacia gum, or raffinose with betaine and glycogen which conserve a higher activity.
An identical analytical process was followed by depositing on each well of a multiwell polystyrene plate Nunc brand the said reaction mixture containing the oligonucleotide primers, drying the plate by introducing it in a desiccator and creating a vacuum by a pump. a plate for each time point and analyzed subsequently temperature was prepared. Also they were prepared for each experiment fresh wells undried reference activity. The amplification conditions and results analysis were identical to those described above. The results of measuring the activity of the dried sample fully coincide with those obtained for the case of the previously mentioned tubes.
EXAMPLE VI Drying tube and on a multiwell plate of a reaction mixture including oligonucleotide primers for the specific amplification of a particular DNA sequence is subsequently subjected to restriction analysis on a multiwell plate containing desiccated in each of the wells the restriction enzymes needed for that analysis
This system was tested using a method of identification and typing of papillomaviruses. Which method comprises a first step of co-amplification of two regions of the viral genome: a 450 bp Ll conserved region in the genome of all papillomaviruses, and a fragment of 250 bp region E6-E7 that present only in oncogenic papillomaviruses. The amplification mixture containing 75 mM Trizma base, 20 mM ammonium sulfate, 0.01% Tween 20, 2 mM MgCl 2, 0.2 mM dATP, dCTP
0.2 mM, 0.2 mM dGTP, 0.2 mM dTTP, 2.5 pmol of primers:
VI-1: 5'-GCMCAGGGWCATAAYAATGG-3 '(SEQ ID NO:.. 16), and
VI-2: 5'-CGTCCMARRGGAWACTGATC-3 '(SEQ ID NO:.. 17),
1.25 pmol of the primers: VI-3: 5'-TGTCAAAAACCGTTGTGTCC-3 '(SEQ ID NO:.. 18), and
VI-4: 5'-GAGCTGTCGCTTAATTGCTC-3 '(SEQ ID NO:.. 19), and
1 unit of DNA polymerase enzyme indicated in Example
I. Analysis of the products of this amplification can identify the presence of papillomaviruses (450 bp band) and determine whether there oncogenic species (250 bp). Then digestion with restriction enzymes 5 (Rsa I, Acc I, Ava I, Ava II and Bgl II) to characterize the species of papillomavirus present in each sample.
Thus, the amplified 250 bp band (corresponding to oncogenic genome) HPV 16, HPV 18 and HPV 33 is digested only by Ava II, rendering each a pattern different restriction easily differentiated on agarose gels, of
HPV 31 is digested by Rsa I, that of HPV 35 by Ava I, of
HPV 52b by Bgl II and HPV 58 Acc I. is also possible to classify the non-oncogenic genotypes by digestion pattern of the 450 bp band with the Rsa I activity
Several tubes as described above, and each of them were added different volumes of the stabilizing mixtures indicated in Table 1. tubes were also prepared with each of the restriction enzymes mentioned above (1 unit of each enzyme were prepared by tube) and 1 microliter of 10X buffers respective reaction. All tubes thus prepared were dried in a centrifugal evaporator Eppendorf 5301, at temperatures between 10 ° C and 60 ° C, for a period of time between 30 and 120 minutes. The temperatures and times mentioned above vary according to the stabilizing mixture used. After drying, the tubes were conserved at the temperatures and times indicated in Table 1. Once the conservation periods indicated in said Table 1 after their activity was assayed after rehydrating the dried mixture with 45 microliters of bi-distilled water and 25 ng of DNA extracted from a patient infected with HPV 18 patient, to a final volume of 50 microliters. 30 cycles of denaturation (94 ° C, 30 seconds), annealing (50 ° C, 1 minute) and extension (72 ° C, 1 minute) were performed using a thermocycler Eppendorf Mastercycler. An electrophoresis result of the amplification reaction in 2% agarose gel (w / v) was performed and was found in all cases in which the amplification was positive, 2 bands of 250 and 450 bp respectively. The activity of the dried reaction mixture was measured by densitometry of the band resulting from the amplification, using an image analyzer TDI Gelprinter using the Gelsuper computer program also developed by TDI. It was considered that the dried mixtures had: maximum activity (+++ in Table 1) when the intensity of the band was 90% ± 10% with respect to the fresh mixture, optimum activity (++ in Table 1) when the activity ranged between 90% and 50%, low activity (+ in Table 1) when it was less than 50%, and absence of activity (- in Table 1) when the result of the amplification reaction was negative.
Then the tubes and wells dried with restriction enzymes were resuspended with 10 microliters of amplifications performed with fresh mixture. After incubating the mixtures at 37 ° C for 30 minutes, the digestion products were analyzed in 1.5% agarose. Is considered: optimum activity ( "digestion" in Table 1) complete digestion bands 250 base pairs (bp), average activity ( "partial" in Table 1) partial digestion of the bands, and inactive ( "no cut" in Table 1) complete absence of digestion.
The densiométricos results obtained after carrying out the amplification reactions can be seen in the column "example VI activity" in Table 1. In conclusion, although several stabilizing mixtures can be considered suitable by having conserved a good activity after drying and subsequent storage of the reaction mixture are those containing melezitose or palatinitol, in conjunction with lysine and glycogen or acacia gum, or raffinose with betaine and glycogen which conserve a higher activity.
Regarding the activity of restriction enzymes, optimum results were obtained with the five enzymes used in cases in which the mixture used in the drying of restriction enzyme containing melezitose or palatinitol, in conjunction with lysine and glycogen or rubber arabic, or raffinose with betaine and glycogen. An identical analytical process was followed by depositing on each well of a multiwell polystyrene plate Nunc brand the said reaction mixture containing the oligonucleotide primers, drying the plate by introducing it in a desiccator and creating a vacuum by a pump. a plate for each time point and analyzed subsequently temperature was prepared. Also they were prepared for each experiment fresh wells undried reference activity. The amplification conditions and results analysis were identical to those described above. The results of measuring the activity of the dried sample fully coincide with those obtained for the case of the previously mentioned tubes.
EXAMPLE VII Amplification of multiwell plate with covalently attached oligonucleotides and dried reaction mixture of a particular DNA sequence is then analyzed by hybridization with a biotinylated probe system for amplification and identification of Plasmodium DNA from clinical samples was used. To this polystyrene multiwell plates (Covalink NH Microwells, Nunc) were used. To each of the wells of the plate he had previously been covalently joined by the generic primer Pl um Plasmodi indicated in Example IV, through its 5 'end, and serve as a primer in the subsequent amplification reaction. Each well of the plates was added the reaction mixture described in Example IV and the different stabilizing mixtures according to Example IV are described and in Table 1 as many plates as points of temperature and storage time were prepared they were subsequently tested (Table 1). After the plates were prepared, they were dried by introducing it into a desiccator and creating a vacuum by a pump. The dried samples were rehydrated with 20 microliters of sterile water, and added with 5 microliters of DNA from infected Plasmodi um at a concentration of 10 ng / .mu.l patients. We proceeded then to perform the amplification reaction under conditions described in Example IV, using a thermocycler Eppendorf Gradient for multiwell plates. In all cases an amplification was performed under the same conditions using a plate not dried to be used as an activity reference. After completion of the reaction was removed from each well the reaction mixture, so that in the well was only bound amplification product dual band. Since one of the bands was synthesized by elongation from the generic primer Pl, this band was covalently bound to pocilio. Conversely other DNA band is connected to the first through complementary base pairing. As for subsequent hybridization reaction is necessary to use as substrate DNA single band, we proceeded to wash plates in order to remove the DNA band that is not covalently attached to pocilio and leave only the synthesized band from oligonucleotide Pl. To this reaction buffer whose composition 50% formamide, 5X SSC and 0.1% SDS was prepared. 50 microliters of this mixture were added to each well and incubated for 10 minutes at 80 ° C. Then the mixture was removed and the washing process was repeated three times.
Hybridization reactions were performed using a biotinylated oligonucleotide. For this, each well was incubated with 100 fmol of the probe in buffer hybridization reaction containing 4X SSC, 10X Denhardt's and 200 ug / ml sperm DNA of salmon, for 2 hours at 60 ° C. Then retired the hybridization mixture and each well was washed 2 times with 200 ul of 0.1X SSC dilution, followed by washing in a buffer of 100 mM maleic acid reaction, pH 7.5, 150 mM NaCl and 0.3% Tween 20, and a final wash in a reaction buffer of 100 mM maleic acid blocking, pH 7.5, 150 mM NaCl and 0.1% BSA. After completion of the locks was added to each well 1: 2000 in reaction buffer previously described blocking streptavidin conjugated peroxidase, and incubated at 23 ° C for 45 minutes. Finally the wells were washed three times with 200 microliters of maleic acid reaction buffer 100 mM, pH 7.5, 150 mM NaCl and 0.3% Tween 20 and once with 200 microliters of buffer 100 mM maleic acid reaction, pH 7.5 and 150 mM NaCl. Finally was added to each well 100 microliters of TMB and incubated 10 minutes in the dark. After stopping the reaction, the absorbance at 450 nm was determined, correcting all values with the absorbance value at 655 nm plastic.
It was considered that the dried mixtures had: maximum activity (+++ in Table 1) when the absorbance measurement was 90% ± 10% with respect to the fresh mixture, optimum activity (++ in Table 1) when it oscillated between 90% and 50%, low activity (+ in Table 1) when it was less than 50%, and absence of activity (- in Table 1) when the absorbance measurement was similar to that obtained in wells not they had contained any reaction mixture.
The obtained activity results can be seen in the "Example VII activity" column of Table 1. In conclusion, although several stabilizing mixtures can be considered suitable by having conserved a good activity after drying and subsequently storing the reaction mixture, again They are those containing melezitose or palatinitol, in conjunction with lysine and glycogen or acacia gum, or raffinose with betaine and glycogen which conserve a higher activity.
EXAMPLE VIII
Compartmentalization of sequential reactions by use of mixed reaction systems liquid / gel The transcriptase enzyme thermostable reverse used in this example, as in Example II phase was a recombinant DNA polymerase from Thermus thermophil us with reverse transcriptase activity is enhanced compared to the enzyme indicated in Example I, I expressed in Escherichia coli, property of Biotools B & M Labs, SA (Spain), and purified using a non-chromatographic method developed by the same company (RETROTOOLS cDNA / DNA polymerase). This enzyme has reverse transcriptase activity in the presence of Mn 2+ ions and DNA polymerase activity in the presence of Mg2 + ions, both of exclusive reactions under conventional conditions. After purification, the enzyme was stored at -20 ° C in a storage buffer containing 30 mM Tris HCl pH 8, 25 mM glucose, 0.5 mM PMSF, 0.25% Tween 20 and 0.25% NP 40 . A reaction buffer for performing PCR reaction containing 1.5 mM EGTA and 4 mM MgCl2 was prepared. To each tube 0.2 ml is added 10 .mu.l of the above-described mixture, and the stabilizing mixtures in previous experiments (Examples I-VII) had shown the best activity were added: melezitose or palatinitol in conjunction with lysine or glycogen or gum arabic, or raffinose with betaine and glycogen. The tubes thus prepared were dried in a centrifugal evaporator Eppendorf 5301, at temperatures between 10 ° C and 60 ° C, for a period of time between 30 and 120 minutes. The temperatures and times mentioned above vary according to the stabilizing mixture used. After drying, the tubes were conserved at the temperatures indicated in Table 1. After the conservation periods indicated in said Table 1 activity was tested.
Finally a reaction buffer was prepared for reverse transcription containing 75 mM Tris HCl pH 8, (NH 4) 2 S0 4 200 mM, Tween 20 0.01%, MnCl 2 1.5 mM, 0.125 mM of each dNTP ( dATP, dCTP, dGTP and dTTP), 5 units of Tth DNA polymerase (RETROTOOLS cDNA / DNA polymerase) and 20 pmol of each primer II-l and II-2 (described in Example II) to amplify a fragment of 1122 bp RNA CD8α mouse gene.
For the activity assay was added to all tubes 0.2 ml in which the mixture was dried PCR, 15 ul of the reaction mixture reverse transcription and RNA 100 ng of mouse, adjusting the final volume H 2 0 to 20 .mu.l. In half of the tubes the gelled contents of the tube were resuspended by pipetting, while the other half will not be resuspended. As a control, in all experiments a RT-PCR were included in two steps under the conditions described in Example II, which was not included any stabilizing mixture.
All tubes were subjected to a round of reverse transcription-amplification in a single step, without inclusion of reagents during the process. Said reaction consisted of incubation at 94 ° C for 1 minute followed by an incubation at 60 ° C for 30 minutes. Then the tubes were high temperature to allow the release of the reagents in the gelled mixtures consisting resuspended no incubation at 83 ° C for 5 minutes followed by incubation at 94 ° C for 3 minutes. Finally 40 repeated cycles each of which consisted of a denaturation step at 94 ° C for 45 seconds, an annealing step at 55 ° C for 30 seconds and an elongation step at 72 ° C for 1 minute were performed. After completion of 40 cycles of amplification was carried out one final elongation step at 72 ° C for 7 minutes. The amplification reaction was performed in a thermocycler Eppendorf Mastercycler.
Amplified products were analyzed by agarose gel electrophoresis at 1% (w / v), being in all cases where the reaction was positive, a single band of 1122 bp amplification. The activity of the reaction mixtures tested was determined by densitometry of bands resulting amplification, using an image analyzer TDI Gelprinter using the Gelsuper computer program also developed by TDI. Activity analysis of amplification reactions showed that the reaction was inhibited in all those tubes in which the gelled mixture was resuspended by previously pipetting to perform the reaction reverse transcription, and by contacting the MnCl 2, the EGTA and MgCl. On the contrary, in the tubes in which the gelled mixture is not resuspended, positive results were obtained in a manner similar to that obtained in the reactions of reverse transcription and amplification in two steps intensity. This shows that the mixture of MgCl 2 and EGTA gelled had remained compartmentalized until released by incubation at high temperatures, allowing realization of two sequential reactions excluding each other.
What is claimed
1. A process for preparing a stabilized reaction mixture and totally or partially dried, said mixture comprising at least one enzyme, comprising:
- at least one inert polymer capable of generating. as mesh structure preventing the mobility of the dried reagents;
2. Method according to claim 1, wherein said reaction mixture comprises at least one enzyme involved in an enzymatic reaction, together with all or part of the necessary reagents for carrying out said enzymatic reaction in which intervenes said enzyme.
3. Method according to claim 2, wherein said reaction mixture comprises at least one enzyme involved in an enzymatic reaction, along with all the necessary reagents for carrying out said enzymatic reaction in which said enzyme intervenes .
4. Method according to claim 1, wherein said enzyme is selected from the group consisting of enzymes nucleic acid amplification, both thermostable and thermolabile nucleic acids, both RNA and DNA, restriction enzymes, enzymes involved in the reactions amplification, sequencing or characterization of nucleic acids, and mixtures thereof.
5. Process according to claim 1, wherein said reaction mixture comprises an enzyme selected from the group consisting of enzymes nucleic acid amplification, both thermostable and thermolabile nucleic acids, both RNA and DNA, restriction enzymes, enzymes involved in amplification reactions, sequencing or characterization of nucleic acids, and mixtures thereof, together with all the reagents necessary for performing the enzymatic reaction in which said enzyme intervenes.
6. Method according to claim 1, wherein said container is a reaction tube or well of a multiwell plate.
7. Method according to claim 1, wherein said protective agent against drying is a non-reducing carbohydrate.
8. Process in accordance with claim 7, wherein said protective agent against drying is selected from the group consisting of a non 'reducing disaccharide, a non-reducing trisaccharide, and mixtures thereof.
9. Process according to claim 8, wherein said protective agent against drying is selected from the group consisting of palatinitol (6- -D-glucopyranosyl mannitol), trehalose, raffinose, melezitose, and mixtures thereof.
10. Method according to claim 7, wherein said stabilizing mixture further comprises a non-reducing carbohydrate as a protective agent against drying, glycerol.
11. Method according to claim 1, wherein said inhibitor of the condensation reactions between carbonyl or carboxyl groups and amine or phosphate groups is a competitive inhibitor or a noncompetitive inhibitor.
12. Method according to claim 11, wherein said inhibitor of the condensation reactions between amino carbonyl or carboxyl groups or phosphate groups and is selected from the group consisting of lysine, arginine, tryptophan, betaine, aminoguanidine derivatives and mixtures thereof.
13. Process according to claim 1, wherein said inert polymer capable of generating a mesh-shaped structure preventing the mobility of dried reagents is selected from the group consisting of polyvinylpyrrolidone (PVP), polyethylene glycol (PEG), dextran, starch , the compound called FICOLL, glycogen, acacia gum and mixtures thereof.
14. Method according to claim 1, wherein the removal of all or part of the water present in the aqueous solution obtained in step a) is performed by any conventional drying method.
15. Method according to claim 14, wherein said drying method is selected from lyophilization, drying in fluidized bed, drying at room temperature and atmospheric pressure, drying at room temperature and decreased pressure, drying at a high temperature and atmospheric pressure, and desiccated at high temperature and reduced pressure.
16. Method according to claim 15, wherein said drying method is drying method will at a temperature between 15 ° C and 60 ° C, and subatmospheric pressure decreased.
17. Process according to claim 1, wherein the stabilized reaction mixture, totally or partially dried, obtained has a moisture content not exceeding 30%.
18. Process according to claim 1, wherein the stabilized reaction mixture, totally or partially dried, obtained has a moisture content of between 1% and 20%.
19. A mixture of reaction stabilized and totally or partially dried, comprising at least one enzyme, and a stabilizing mixture comprising (i) at least one protective agent against drying, (ii) at least one inhibitor the condensation reaction between amino carbonyl or carboxyl groups and phosphate groups, and (iii) at least one inert polymer capable of generating a mesh-shaped structure preventing the mobility of the dried reagents.
20. Mix reaction stabilized and totally or partially dried according to claim 18 having a moisture content not exceeding 30%.
21. Mix reaction stabilized and totally or partially dried, according to claim 18 having a degree of humidity between 1% and 20%.
22. Mix reaction stabilized and totally or partially dried, according to claim 18, wherein said enzyme is selected from the group consisting of enzymes nucleic acid amplification, restriction enzymes, enzymes involved in amplification reactions, sequencing or characterization of nucleic acids, and mixtures thereof.
23. Mix reaction stabilized and totally or partially dried, according to claim 18, comprising an enzyme selected from the group consisting of enzymes nucleic acid amplification, restriction enzymes, enzymes involved in amplification reactions, sequencing or characterization nucleic acids, and mixtures thereof, together with all or part of the necessary reagents for carrying the enzymatic reaction in which said enzyme intervenes.
24. Mix reaction stabilized and totally or partially dried, according to claim 23, wherein said reagents necessary for performing the enzymatic reaction in which said enzyme intervenes are selected from the group consisting of cofactors; enzyme substrates; additives enhancing or modulating the enzymatic reactions; dNTPs; ddNTPs; oligonucleotide primers and probes, optionally labeled; and mixtures thereof.
25. Mix reaction stabilized and totally or partially dried, according to claim 18 comprising a DNA polymerase enzyme or enzyme reverse transcriptase, thermostable or not, deoxynucleotide triphosphates, optionally labeled, the cofactors required for enzyme activity and optionally , additives enhancing or modulating said enzymatic activity.
26. Mix reaction stabilized and totally or partially dried, according to claim 25, further comprising oligonucleotide primers reaction, optionally labeled, necessary for the specific amplification of a nucleotide target sequence, and / or probes, optionally labeled necessary for performing a hybridization assay, optionally together with any additive or coadjuvant of the hybridization reaction.
27. Mix reaction stabilized and totally or partially dried, according to claim 18, which further comprises dideoxyribonucleotides (ddNTPs).
28. Mix stabilized and totally or partially dried reaction according to claim 18, comprising a restriction enzyme, together with, optionally, all the cofactors and additives necessary for carrying out a restriction analysis.
29. Mix reaction stabilized and totally or partially dried, according to claim 18, presented in an embodiment "ready to use" (ready-to-use).
30. Mix reaction stabilized and totally or partially dried, according to claim 18, wherein said protective agent against drying is a non-reducing carbohydrate.
31. Mix reaction stabilized and totally or partially dried, according to claim 30, wherein said protective agent against drying is selected from the group consisting of a non-reducing disaccharide, a non-reducing trisaccharide, and mixtures thereof.
32. Mix reaction stabilized and totally or partially dried, according to claim 30, wherein said protective agent against drying is selected from the group consisting of palatinitol (6-α-D-glucopyranosyl-mannitol), trehalose, raffinose, melezitose, and mixtures thereof.
33. Mix reaction stabilized and totally or partially dried, according to claim 18, wherein said stabilizing mixture further comprises a non-reducing carbohydrate as a protective agent against drying, glycerol.
34. Mix reaction stabilized and totally or partially dried, according to claim 18, wherein said inhibitor of the condensation reactions between carbonyl or carboxyl groups and amine or phosphate groups is a competitive inhibitor or a noncompetitive inhibitor.
35. Mix reaction stabilized and totally or partially dried, according to claim 34, wherein said inhibitor of the condensation reactions between carbonyl or carboxyl groups and amine or phosphate groups is selected from the group consisting of lysine, arginine, tryptophan, betaine , aminoguanidine derivatives and mixtures thereof.
36. Mix reaction stabilized and totally or partially dried, according to claim 18, wherein said inert polymer capable of generating a mesh-shaped structure preventing the mobility of dried reagents is selected from the group consisting of polyvinylpyrrolidone (PVP) , polyethylene glycol (PEG), dextran, starch, the compound called FICOLL, glycogen, acacia gum and mixtures thereof.
37. A kit comprising a mixture of reaction stabilized and totally or partially dried, according to any of claims 18 to 36.
PCT/ES2002/000109 2001-03-12 2002-03-11 Method for preparing stabilised reaction mixtures, which are totally or partially dried, comprising at least one enzyme, reaction mixtures and kits containing said mixtures WO2002072002A2 (en)
ESP0100569 2001-03-12
ES200100569A ES2180416B1 (en) 2001-03-12 2001-03-12 Process for preparing stabilized mixtures, totally or partially dried reaction, comprising at least one enzyme, reaction mixtures and kits containing them.
EP02714205.8A EP1374827B1 (en) 2001-03-12 2002-03-11 Method for preparing stabilised reaction mixtures, which are totally or partially dried, comprising at least one enzyme, reaction mixtures and kits containing said mixtures
CA 2408857 CA2408857A1 (en) 2001-03-12 2002-03-11 Method for preparing stabilised reaction mixtures, which are totally or partially dried, comprising at least one enzyme, reaction mixtures and kits containing said mixtures
BR0204479A BR0204479B1 (en) 2001-03-12 2002-03-11 Process for preparing stabilized mixtures that reaÇço total SCO or partially dried, comprising at least one enzyme, reaÇço mixtures and kits containing said mixtures
AU2002246125A AU2002246125B2 (en) 2001-03-12 2002-03-11 Process for preparing stabilized reaction mixtures which are partially dried, comprising at least one enzyme, reaction mixtures and kits containing said mixtures
JP2002570962A JP4448280B2 (en) 2001-03-12 2002-03-11 Partially method for producing a stabilized mixture containing one or more enzymes dry mixture, and the mixture kit containing
IL15275702A IL152757A (en) 2001-03-12 2002-03-11 Process for preparing stabilised reaction mixtures, which are partially dried, comprising at least one enzyme, reaction mixtures and kits containing said mixtures
US10/292,848 US7919294B2 (en) 2001-03-12 2002-11-12 Process for preparing stabilized reaction mixtures which are partially dried, comprising at least one enzyme, reaction mixtures and kits containing said mixtures
US10/292,848 Continuation US7919294B2 (en) 2001-03-12 2002-11-12 Process for preparing stabilized reaction mixtures which are partially dried, comprising at least one enzyme, reaction mixtures and kits containing said mixtures
WO2002072002A2 true WO2002072002A2 (en) 2002-09-19
WO2002072002A3 WO2002072002A3 (en) 2002-11-28
PCT/ES2002/000109 WO2002072002A2 (en) 2001-03-12 2002-03-11 Method for preparing stabilised reaction mixtures, which are totally or partially dried, comprising at least one enzyme, reaction mixtures and kits containing said mixtures
Country Status (10)
KR (1) KR20030036178A (en)
AU (1) AU2002246125B2 (en)
BR (1) BR0204479B1 (en)
ES (1) ES2180416B1 (en)
IL (1) IL152757A (en)
ES2214144A1 (en) * 2003-02-26 2004-09-01 BIOTOOLS BIOTECHNOLOGICAL & MEDICAL LABORATORIES, S.A. Stabilized composition fluorimetric assays, colorimetric or chemo-luminescent, kits containing it and process for its obtention.
WO2011048227A1 (en) 2009-10-22 2011-04-28 Biotools Biotechnological & Medical Laboratories, S.A. Composition, method and kit for detecting bacteria by means of sequencing
JP2011250792A (en) * 2004-04-07 2011-12-15 Access Bio Inc Nucleic acid detection system
WO2013017702A1 (en) 2011-08-01 2013-02-07 2B Blackbio S.L. Method for determining the genotype of multiple sequences
US6893877B2 (en) 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
US20020151040A1 (en) 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
US8277753B2 (en) 2002-08-23 2012-10-02 Life Technologies Corporation Microfluidic transfer pin
CA2521999A1 (en) 2002-12-20 2004-09-02 Biotrove, Inc. Assay apparatus and method using microfluidic arrays
DE102004007526A1 (en) * 2004-02-17 2005-09-01 Oetjen, Georg-Wilhelm, Dr. Method and apparatus for freeze-drying of products
US8105554B2 (en) 2004-03-12 2012-01-31 Life Technologies Corporation Nanoliter array loading
EP3167961A1 (en) 2004-04-08 2017-05-17 Biomatrica, Inc. Integration of sample storage and sample management for life science
US20060099567A1 (en) * 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
GB0414815D0 (en) * 2004-07-02 2004-08-04 Secr Defence Method for stabilising reagents which are useful for nucleic acid amplification
ES2318575T3 (en) * 2004-11-10 2009-05-01 Novartis Vaccines And Diagnostics, Inc. Deamidated Interferon beta.
US20060242012A1 (en) * 2005-04-22 2006-10-26 Sumit Agarwal Determining or scoring properties to solicit to join ad network using advertiser or aggregated advertiser interest
US20070031914A1 (en) * 2005-08-05 2007-02-08 Wei Zhu Devices for analyte assays and methods of use
EP2021508B1 (en) * 2006-05-23 2012-04-25 Molecular Detection, Inc. Ambient temperature stable kits for molecular diagnostics
EP1870649A1 (en) 2006-06-20 2007-12-26 Octapharma AG Lyophilisation targetting defined residual moisture by limited desorption energy levels
AU2007297484B2 (en) * 2006-09-18 2013-07-18 Ge Healthcare Bio-Sciences Corp. Preparation of glassified biological reagents
DE102006056790B3 (en) * 2006-12-01 2008-01-17 IfP Privates Institut für Produktqualität GmbH Kit for carrying out a polymerase chain reaction comprises reaction vessels loaded by drying aqueous solutions of the reagents in the presence of trehalose alone
US20100015628A1 (en) * 2007-02-28 2010-01-21 Ge Healthcare Bio-Sciences Corp. Ambient temperature stable chemical/biological reagents on membranes or filters
EP2207893B1 (en) 2007-10-19 2011-12-28 Becton, Dickinson and Company Methods for the detection of beta-lactamases
KR100978215B1 (en) * 2008-02-28 2010-08-26 주식회사 인트론바이오테크놀로지 Kit suitable for screening and establishment of optimal amplification condition in PCR constructed with dried-formulated PCR reagent and method for producing the same
JP5677972B2 (en) 2008-11-18 2015-02-25 メリマック ファーマシューティカルズ インコーポレーティッド Human serum albumin linkers and conjugates thereof
WO2009126920A2 (en) * 2008-04-11 2009-10-15 Merrimack Pharmaceuticals, Inc. Human serum albumin linkers and conjugates thereof
KR101008335B1 (en) * 2008-05-28 2011-01-14 주식회사 썬제네틱스 Reaction Mixtures for Nucleic Acid?Involving Reaction Catalyzing Enzymes
WO2010047778A1 (en) 2008-10-20 2010-04-29 Becton Dickinson And Company Compositions for the detection of intracellular bacterial targets and other intracellular microorganism targets
WO2010107112A1 (en) * 2009-03-19 2010-09-23 アークレイ株式会社 Method for preventing reduction in activity of enzyme during drying
WO2010144682A1 (en) 2009-06-12 2010-12-16 Micronics, Inc. Rehydratable matrices for dry storage of taq polymerase in a microfluidic device
WO2010144683A2 (en) * 2009-06-12 2010-12-16 Micronics, Inc. Compositions and methods for dehydrated storage of on-board reagents in microfluidic devices
WO2011151473A1 (en) * 2010-06-02 2011-12-08 2B Blackbio S.L. Composition, method and kit for detecting fungi and yeasts by means of sequencing
JP5933544B2 (en) 2010-07-26 2016-06-08 バイオマトリカ, インコーポレーテッド Blood during shipping and storage at ambient temperature dna, compositions for stabilizing rna and proteins and other biological samples
US9845489B2 (en) 2010-07-26 2017-12-19 Biomatrica, Inc. Compositions for stabilizing DNA, RNA and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
KR20140143139A (en) * 2012-03-08 2014-12-15 소니 주식회사 Method for producing microchip for use in nucleic acid amplification reaction
CN102703428B (en) * 2012-06-01 2013-09-18 中国水产科学研究院黄海水产研究所 Gel-based preservation method for polymerase chain reaction agent and reaction reagent
CN102703427B (en) * 2012-06-01 2013-11-06 中国水产科学研究院黄海水产研究所 Gel-based preservation method for nucleic acid isothermal amplification reagent
CN102703429B (en) * 2012-06-01 2013-09-18 中国水产科学研究院黄海水产研究所 Nucleic acid isothermal amplification reaction reagent suitable for being stored and transported at normal temperature
US20150202287A1 (en) 2012-08-30 2015-07-23 Merrimack Pharmaceuticals, Inc. Combination therapies comprising anti-erbb3 agents
US9725703B2 (en) 2012-12-20 2017-08-08 Biomatrica, Inc. Formulations and methods for stabilizing PCR reagents
CN106572650A (en) 2014-06-10 2017-04-19 生物马特里卡公司 Stabilization of thrombocytes at ambient temperatures
US9868944B2 (en) 2014-12-19 2018-01-16 Roche Molecular Systems, Inc. Reaction mixtures
WO2017136782A1 (en) * 2016-02-05 2017-08-10 Mark Filipowsky Dried amplification compositions
RU2016114990A (en) * 2016-04-18 2017-10-20 Общество С Ограниченной Ответственностью "Ниармедик Плюс" The stabilized mixture of reagents for molecular diagnostics
WO2018226569A1 (en) * 2017-06-06 2018-12-13 Danisco Us Inc Use of betaine to stabilize and/or increase the activity of enzymes in stressful environments
EP0091258A2 (en) 1982-04-07 1983-10-12 Asahi Kasei Kogyo Kabushiki Kaisha Method for stabilizing tumor necrosis factor and a stable aqueous solution or powder containing said factor
EP0140489A1 (en) 1983-08-05 1985-05-08 Wako Pure Chemical Industries, Ltd. Method for stabilizing immunologically active substances immobilized on an insoluble carrier and their use in the preparation of reagents for measuring physiologically active substances
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US4891319A (en) 1985-07-09 1990-01-02 Quadrant Bioresources Limited Protection of proteins and the like
WO1991018091A1 (en) 1990-05-14 1991-11-28 Quadrant Holdings Cambridge Limited Stabilization of biological macromolecular substances and other organic compounds
WO1993000807A1 (en) 1991-07-03 1993-01-21 Cryolife, Inc. Method for stabilization of biomaterials
WO1996005809A1 (en) 1994-08-19 1996-02-29 Quadrant Holdings Cambridge Limited Improved method for stabilization of biological substances during drying and subsequent storage and compositions thereof
US5614387A (en) 1995-02-10 1997-03-25 Gen-Probe Incorporated Method of making stabilized enzyme compositions for nucleic acid amplification
US5614437A (en) 1995-01-26 1997-03-25 Lsi Logic Corporation Method for fabricating reliable metallization with Ta-Si-N barrier for semiconductors
US5861251A (en) 1996-10-15 1999-01-19 Bioneer Corporation Lyophilized reagent for polymerase chain reaction
US5935834A (en) 1994-11-11 1999-08-10 Asahi Kasei Kogyo Kabushiki Kaisha Reverse transcriptase composition having improved storage stability
WO2001004135A2 (en) 1999-07-13 2001-01-18 The Regents Of The University Of Michigan Crosslinked dna condensate compositions and gene delivery methods
US2992237A (en) * 1961-07-11 Process for the production of com-
GB1261711A (en) * 1968-02-15 1972-01-26 Astra Ab Process for producing a penicillin acylose composition
US3892876A (en) * 1971-11-30 1975-07-01 Leiner & Sons Wales Limited P Process of preparing freeze-dried gelatin
GB9417211D0 (en) * 1994-08-25 1994-10-12 Solicitor For The Affairs Of H Nucleotide sequencing method
US5565318A (en) * 1994-09-02 1996-10-15 Pharmacia Biotech, Inc. Room temperature stable reagent semi-spheres
US6290991B1 (en) * 1994-12-02 2001-09-18 Quandrant Holdings Cambridge Limited Solid dose delivery vehicle and methods of making same
US6964771B1 (en) * 1995-06-07 2005-11-15 Elan Drug Delivery Limited Method for stably incorporating substances within dry, foamed glass matrices
US6150094A (en) * 1996-05-23 2000-11-21 Qiagen Gmbh Use of an osmolyte for reducing or abolishing no-covalent interactions of biological molecules to inert surfaces
US5876992A (en) * 1996-07-03 1999-03-02 Molecular Biology Resources, Inc. Method and formulation for stabilization of enzymes
US6136578A (en) * 1997-03-12 2000-10-24 Novo Nordisk A/S Storage-stable liquid formulation comprising a laccase
US6436897B2 (en) * 1998-06-01 2002-08-20 Celtrix Pharmaceuticals, Inc. Pharmaceutical formulations for IGF/IGFBP
US6225289B1 (en) * 1998-12-10 2001-05-01 Genvec, Inc. Methods and compositions for preserving adenoviral vectors
US6300073B1 (en) * 1999-10-01 2001-10-09 Clontech Laboratories, Inc. One step RT-PCR methods, enzyme mixes and kits for use in practicing the same
2001-03-12 ES ES200100569A patent/ES2180416B1/en active Active
2002-03-11 IL IL15275702A patent/IL152757A/en not_active IP Right Cessation
2002-03-11 BR BR0204479A patent/BR0204479B1/en not_active IP Right Cessation
2002-03-11 AU AU2002246125A patent/AU2002246125B2/en not_active Ceased
2002-03-11 WO PCT/ES2002/000109 patent/WO2002072002A2/en active Application Filing
2002-03-11 KR KR10-2002-7015148A patent/KR20030036178A/en not_active Application Discontinuation
US4683202B1 (en) 1985-03-28 1990-11-27 Cetus Corp
US5955448A (en) 1994-08-19 1999-09-21 Quadrant Holdings Cambridge Limited Method for stabilization of biological substances during drying and subsequent storage and compositions thereof
ARCHIVES BIOCHEM. BIOPHYSICS, vol. 232, 1984, pages 400 - 497
CARPENTER ET AL., CRYOBIOLOGY, vol. 24, 1987, pages 455 - 464
CHIEN ET AL., J.BACTERIOL, vol. 127, 1976, pages 1550 - 1557
CLEGG, CRYOBIOLOGY, vol. 19, 1982, pages 306 - 316
COLACO ET AL., BIO/TECHNOL., vol. 10, 1992, pages 1007 - 1011
CROWE, BIOCHIMIA AND BIOPHYSICA ACTA, vol. 769, 1984, pages 141 - 150
CRYOBIOLOGY, vol. 20, 1983, pages 346 - 356
IGAKI, J. JPN. DIABETES SOC, vol. 34, 1991, pages 403 - 407
J. SAMBROCK ET AL.: "Molecular Cloning: A Laboratory Manual, 2nd Ed.", 1989
JONES ET AL., NUCLEIC ACIDS RESEARCH, vol. 17, 1989, pages 8387 - 8388
KALEDIN ET AL., BIOKHIMIYA, vol. 45, 1980, pages 644 - 651
KARKAS ET AL., PROC. NATL. ACAD. SCI. USA, vol. 70, 1973, pages 3834 - 3838
KWOK, S. ET AL., NATURE, vol. 339, 1989, pages 237 - 238
LEOB ET AL., NATURE NEW BIOL., vol. 242, 1973, pages 66 - 69
MATTHEWS, FASEB J., vol. 10, 1996, pages 35 - 41
MEN6NDEZ-ARIAS; ARGOS, J. MOL. BIOL., vol. 206, 1989, pages 397 - 406
MOCHARLA ET AL., GENE, vol. 93, 1990, pages 271 - 275
O. TOORU, CHEMICAL ABSTRACTS, vol. 95, 1981, pages 517
PACE ET AL., FASEB J., vol. 10, 1996, pages 75 - 83
PIKAL M.J., BIOPHARM, vol. 3, 1990, pages 18 - 20,22-23,26-27
RENTIER-DELRUE ET AL., J. MOL. BIOL., vol. 229, pages 85 - 93
ROSER B., BIOPHARM, vol. 4, 1991, pages 47 - 53
RUTTIMANN ET AL., EUR. J. BIOCHEM, vol. 149, 1985, pages 41 - 46
SAIKI ET AL., SCIENCE, vol. 230, 1985, pages 1350 - 1354
SAIKI ET AL., SCIENCE, vol. 239, 1988, pages 487 - 491
WARREN; PETSKO, PROT. ENG., vol. 8, 1995, pages 905 - 913
WATANABE ET AL., J. BIOL. CHEM., vol. 266, 1991, pages 24287 - 24294
YIP ET AL., STRUCTURE, vol. 3, 1995, pages 1147 - 1158
WO2004076656A1 (en) * 2003-02-26 2004-09-10 Biotools Biotechnological & Medical Laboratories, S.A. Stabilised composition for fluorimetric, colorimetric or chemoluminescent assays, kits containing same and production method thereof
ES2180416A1 (en) 2003-02-01
AU2002246125B2 (en) 2008-02-07
ES2180416B1 (en) 2004-06-01
BR0204479A (en) 2004-02-17
IL152757A (en) 2009-02-11
IL152757D0 (en) 2003-06-24
KR20030036178A (en) 2003-05-09
BR0204479B1 (en) 2013-07-16
EP0527728B1 (en) 1997-07-09 (in vitro) dna synthesis reactions using modified phi 29 dna polymerase and a dna fragment encoding said polymerase
EP1130118B1 (en) 2006-01-04 Amplification of long nucleic acid sequences by PCR
EP0592035B1 (en) 1996-01-03 Thermostable DNA polymerase composition comprising a temperature sensitive polymerase inhibitor, diagnostic test kits and methods of use
JP3026554B2 (en) 2000-03-27 Thermostable enzyme
CA2365135C (en) 2009-08-18 Method for amplifying nucleic acid sequence
US6294365B1 (en) 2001-09-25 Method and formulation for stabilization of enzymes
US6197563B1 (en) 2001-03-06 Kits for amplifying and detecting nucleic acid sequences
JP4053363B2 (en) 2008-02-27 Hot start nucleic acid amplification compositions and hot start nucleic acid amplification method
AU2001268725B2 (en) 2006-08-03 Multiply-primed amplification of nucleic acid sequences
Gelfand 1989 Taq DNA polymerase
US20010041334A1 (en) 2001-11-15 Stable compositions for nucleic acid amplification and sequencing
JP3727667B2 (en) 2005-12-14 Amplification and detection of nucleic acids in blood samples
US5773257A (en) 1998-06-30 Method for producing primed nucleic acid templates
US6300073B1 (en) 2001-10-09 One step RT-PCR methods, enzyme mixes and kits for use in practicing the same
JP4777497B2 (en) 2011-09-21 Generation and amplification of nucleic acids from ribonucleic acids
CA2017522C (en) 1996-06-18 Process for controlling contamination of nucleic acid amplification reactions
US20030082567A1 (en) 2003-05-01 Mineral hot start PCR method
US20020173016A1 (en) 2002-11-21 High-throughput nucleic acid polymerase devices and methods for their use
JP3655240B2 (en) 2005-06-02 Thermostable enzyme that promotes the fidelity of thermostable dna polymerase for improved nucleic acid synthesis and amplification in vitro
JP3673175B2 (en) 2005-07-20 Stabilization of enzymes by cationic surfactants
EP1463808B1 (en) 2009-02-11 High fidelity dna polymerase compositions and uses therefor
Lowery et al. 1977 Characterization of the nucleoside triphosphate phosphohydrolase (ATPase) activity of RNA synthesis termination factor p. II. Influence of synthetic RNA homopolymers and random copolymers on the reaction.
US6153412A (en) 2000-11-28 Lyophilized reagent for polymerase chain reaction
US5614387A (en) 1997-03-25 Method of making stabilized enzyme compositions for nucleic acid amplification
US20100196904A1 (en) 2010-08-05 Ambient temperature stable kits for molecular diagnostics
2002-09-19 AL Designated countries for regional patents
Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG
2002-09-19 AK Designated states
Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG US UZ VN YU ZA ZM ZW
2002-11-05 WWE Wipo information: entry into national phase
Ref document number: 2002246125
Country of ref document: AU
Country of ref document: IL
Ref document number: 10292848
Country of ref document: US
Ref document number: 2408857
Country of ref document: CA
Ref document number: 1020027015148
Country of ref document: KR
2002-11-13 121 Ep: the epo has been informed by wipo that ep was designated in this application
2002-12-05 REEP
Country of ref document: EP
2003-05-09 WWP Wipo information: published in national office
2004-01-29 REG Reference to national code
Ref legal event code: 8642