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By Emily Tillett CBS News November 20, 2019, 12:16 PM
Pence denies Gordon Sondland's claims that he knew of Ukraine dealings
Pence says U.S. will be "unrelenting" in fight against ISIS
Vice President Mike Pence is disputing EU Ambassador Gordon Sondland's assertion that Pence, along with other top administration officials, was keenly aware of a campaign to pressure Ukraine to probe the Ukrainian-based energy company Burisma, the Bidens and Ukraine's role in the 2016 election as part of a quid pro quo arrangement, effectively tying up military aid to Ukraine.
In a statement released by the vice president's office, Pence's Chief of Staff Marc Short said the vice president "never had a conversation with Gordon Sondland about investigating the Bidens, Burisma, or the conditional release of financial aid to Ukraine based upon potential investigations."
"Ambassador Gordon Sondland was never alone with Vice President Pence on the September 1 trip to Poland. This alleged discussion recalled by Ambassador Sondland never happened," the statement added in reference to Sondland's public testimony before the House Intelligence Committee as part of their ongoing impeachment probe of President Trump.
In Wednesday's testimony, Sondland implicated both Pence and Secretary of State Mike Pompeo of having full knowledge of plans pushed by Mr. Trump attorney Rudy Giuliani to carry out an investigation of the former vice president and his son, as well as a debunked conspiracy theory that Ukraine was somehow involved in the election interference campaign in 2016.
Sondland also testified that he had mentioned to Pence directly his concerns in delaying key military aid to Ukraine as part of a quid pro quo arrangement to carry out Giuliani's investigations. He said the conversation happened on the sidelines of a meeting with Ukrainian President Volodymyr Zelensky in Warsaw.
"I mentioned to Vice President Pence before the meetings with the Ukrainians that I had concerns that the delay in aid had become tied to the issue of investigations," Sondland testified. "I recall mentioning that before the Zelensky meeting."
"This security aid was critical to Ukraine's defense and should not have been delayed. I expressed this view to many during this period. But my goal, at the time, was to do what was necessary to get the aid released, to break the logjam. I believed that the public statement we had been discussing for weeks was essential to advancing that goal," Sondland testified.
The written statement was the first time Pence's office directly answered a question about whether the vice president was aware of the request for investigations.
It's a point Pence neither confirmed nor denied during his appearance on "Face the Nation" last month. Asked four separate times if he was aware of the deal, Pence repeatedly changed course and reiterated there was "no quid pro quo" on the president's part.
"He did nothing wrong," Pence claimed.
"But are you saying that you did not ever hear of such a deal? Is that what I understand you're describing?" "Face the Nation" moderator Margaret Brennan pressed.
"I'm telling you that all of my interactions with the president, all of my conversations with President Zelensky, were entirely focused on issues of importance to the American people, ending corruption, enlisting more European support and supporting Ukraine in a way that would restore its territorial integrity and stand by Ukraine for its sovereignty," Pence replied.
Emily Tillett On Twitter»
Emily Tillett is the digital producer at "Face the Nation"
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DMangelo = Conscious Hip Hop's Finest
DMangelo's new song "White Brick White House" dives into the horrors of drug addiction and abuse. The music video, which was shot in Colombia, has a dark cast over it and showcases how the romanticization of drugs and alcohol have contributed to an epidemic in modern culture.
The style of the beat sounds slightly haunted setting the perfect mood for the lyrics. DMangelo delivers his rap with great flow and diction over the track. He lays out the truths about drug addiction and the governments involvement. There is not one point on the track where we feel like DMangelo holds back. This is conscious hip hop at it's finest.
DMangelo says "It's inspired by the dark tension between those who struggle and those who profit." That message comes across really clear through his lyrical content and delivery. This is a very important topic that cannot be ignored. Kudos to DMangelo for speaking up through the art of music.
"After traveling to Colombia and meeting with the Venezuelans, and Peruvians, and not only hearing but seeing what cocaine did to them as a people. Issues with the cartels, pain, poverty, and segregation. It felt like, I was back in the projects, where those who risk it all either win it all, or die trying. The Colombians, Venezuelans, and Peruvians had the same feelings and drive. They also had the same government profiting off of them every step of the way. We all agreed its no drug trade without government involvement. You know, white brick white house. Shot the video the next day, it was crazy. The original video was a lot more graphic, we was really in they trap and it was so much shit going down, but I dint want to risk people being seen on camera, and/or being put in jeopardy."
Dive into the music video on YouTube now:
More info: dmangelo.com
Posted in Culture, Hip Hop, new release, RapTagged dmangelo, Hip Hop
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Home Crime and Punishment BREAKING: Zimmerman Prosecution Opens with "Fucking Punks"
BREAKING: Zimmerman Prosecution Opens with "Fucking Punks"
"Fucking punks. These assholes they always get away." That's how Assistant DA John Guy began his opening statement in the murder trial of George Zimmerman. Allegedly quoting the defendant, Guy uttered the phrase five times. Apparently, Zimmerman said it "just before he pulled the trigger on Trayvon Martin." [Click here for live coverage.] The judge has banned the words "racial profiling" from the opening statement. The Prosecutor got around the stricture by stating that Zimmerman was looking "for people who didn't belong" in his 'hood. Bernie also told the jury that the bullet that killed Martin was "a contact shot." Possible with a semi? Or who cares? Plenty of gun stuff to come. Meanwhile, it looks like the State's case depends on . . .
witnesses not corroborating Zimmerman's version of events. Bernie's statement relied a great deal on contradicting Zimmerman's "story" by repeatedly pointing out that "no one saw that" and "no one heard that." A lack of evidence is evidence but it's not always the most convincing evidence, IMHO.
The prosecution's opening statement continued with Guy promising to attack Zimmerman's character, portraying the defendant as a cop wanne-be who didn't follow Neighborhood Watch protocol (as spelled out by materials presented to Zimmerman). "Don't follow. Don't confront. Just call and observe."
The "p" bird eventually reared its ugly head—within the judge's guidelines—with this: "He profiled him [Martin] as someone who wanted to commit a crime in his neighborhood."
Guy closes with "You will know in your head, you will know in your heart and you will know in your stomach that George Zimmerman didn't shoot Trayvon Martin because he had to. He shot him for the worst of all reasons: because he wanted to."
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St. Louis Homeowner Sends Five Home Invaders Running For Their Lives, One to the Morgue [VIDEO]
PhoenixNFA June 24, 2013 At 09:39
Sounds like the courtroom in "idiocracy"
Adam June 24, 2013 At 10:47
We got, like, all this evidence and shit.
But yo, check it. He is guilty. Peace.
Jarhead1982 June 24, 2013 At 12:04
But yo, you aint judge jury and executioner and yo wont dere!
Anonymous June 24, 2013 At 13:12
They need to get Zimmerman an identification tatoo on his arm that says "Sure, Not"
They need to follow all this up with Butt Fvckers catering in the courtroom while watching the movie "Ass"
The DA would probably throw out the phrase, "Go away! 'Batin'!" somewhere in there.
Rambeast June 24, 2013 At 17:06
On the next episode of "Ow, my balls"!
Foster Alley June 24, 2013 At 09:44
Note to self: It ain't about race.
Jacen June 24, 2013 At 10:57
It's always about race. Most of us are just uncomfortable to bring it up when it's in court.
When the facts are on your side, pound on the facts.
When the law is on your side, pound on the law.
When neither the facts nor the law are on your side, pound on the table.
This is what table-pounding looks like.
DerryM June 24, 2013 At 10:03
Accur81 June 24, 2013 At 11:23
Well said, sir.
Tommy Knocker June 24, 2013 At 09:49
This Colby lookalike also pointed out with disgust that the Zman had 2 flashlites! That he carried his KILL-TEC (I swear thats what he said) inside his waistband. That it was loaded with a round in the chamber!
This is gonna be fun right up until the urban centers in America explode in race riots.
Colby June 24, 2013 At 10:31
He doesn't really look like me.
jkp June 24, 2013 At 11:16
We won't know for sure until we see his teeth.
So I am a cop all these years having two flashlights in my car, damn, all those arrests I could have made!
JSIII June 24, 2013 At 09:54
Dirtbag prosecutor. Reminds me of the guy from the Duke LaCrosse case.
Saul Feldstein June 24, 2013 At 10:00
Wakeup call, no matter how "gun friendly" you think your state is, a politically minded DA will throw your gun rights down the tubes in a heartbeat to suck up to the system.
This case was pure and simple self defense under existing Florida law, but look what it has become.
This is why gun registries, DNA databases, metadata sweeps and all the rest are the beginning of the end.
Not being able to blatantly play "the race card", the Prosecutor has to imply/inject prejudice on Zimmerman's part into the Jury's minds. Hopefully, they will see this is purely a self-defense case and nothing more, or less.
Jason Coyne June 24, 2013 At 10:23
The contact shot is actually a good thing for the defense. It was a contact shot against the sweatshirt, but an intermediate ("several inches") shot against Martin's body. This means the shirt was not flush against his skin, but hanging away – good evidence that Martin was on top.
Second Amendment June 24, 2013 At 13:07
Excellent point. Wonder if the jury is bright enough to grasp that bit of evidence, though?
Gov. William J. Le Petomane June 24, 2013 At 10:25
Seems to me he's overplaying his hand. Opening up with a string of profanities may turn off the jurors even if Zimmerman had said that, seems a bit unprofessional to me. But if he can't get witnesses to corroborate his claim he's just shot himself in the foot.
Does anyone own a PF9? Under ***extremely safe conditions,*** can someone confirm if a contact shot is possible with that particular pistol.
Per my comment above, it was not a contact shot against Martin's body, so there is no physical impediment to cycling. If a sweatshirt alone is enough to stop the gun from shooting – its time to find another gun.
So 1911's are out?
Ruger LCR .357 Magnum
TTACer June 24, 2013 At 12:04
The question isn't about cycling. Many modern guns have a built-in "safety" that prevents the gun from being fired if the muzzle is pressed against a surface.
Hobbez June 24, 2013 At 10:36
I can personally confirm that, as long as the business end is the only part touching something, that my PF9 will fire when pressed against a target. It is not pleasant, it will remove the hair off your knuckles, and mine experienced a stovepipe every time that I tried but, it fired each time.
If you're having a lot of stovepipes or FTEs on the PF-9, consider adding a second extractor spring on top of the existing one. Solved my FTE problem perfectly. Used to be one FTE every 3 to 5 rounds (!), now magazine after magazine the rounds feed, extract, and eject flawlessly.
Santander June 24, 2013 At 16:24
See — helpful advice is why we read this blog …
Must find spring extractor ….
Yes, a contact shot is possible (though the Zman case isn't a contact shot), since the barrel, slide, and frame all align at the muzzle end of the gun. The frame would thus prevent any rearward displacement of the barrel/slide.
Now, if the pistol were tilted upward while being pressed into a target so that the slide and barrel were pushed back more than about 4mm then the trigger is disengaged and thus it can't fire.
Well, if Zimmerman gets off, be ready for the race riots to follow. I don't know how to feel about that…or where they'll happen. Does anyone know which African-American communities are closely tied to this case?
Stephan Jarrett June 24, 2013 At 11:32
If there is no crime, he isn't "getting off".
Bruce B. June 24, 2013 At 11:56
Sanford is an Orlando bedroom community. Both have large, low income black populations. Was in Sanford last week, have family there. Was informed they have the National Guard on speed dial.
Jim R June 24, 2013 At 10:31
The DA's office knows what'll happen if Zimmerman is acquitted. There'll be riots. Violent, bloody riots the likes of which we haven't seen in this country in 20 years, and all over the nation. He's thinking "If we don't railroad this guy into prison, I'm going to be held responsible for all that chaos and I'm going to lose my job".
Then there will be lots of idiots shot in incidents of valid self defense, and the democrats would just hate that they could only get those now dead constituents to vote only once when dead versus 3 to 6 times when alive!
Matt in FL June 24, 2013 At 11:55
I'll bet my bottom dollar against yours, that these "violent, bloody riots" you speak of will not happen.
A few demonstrations around black churches in black communities. That will be the extent of the wildly imagined "riots".
Agreed. We'll likely see a couple/few "demonstrations," maybe a tiny bit of civil disobedience, but maybe not even that much. And that's it. People who are expecting Watts or Rodney King (RF, I'm looking at you) are out of their minds. This case is just not that big of a deal. Even the ones who are trying to make it about race know in their heart it's not.
JoshinGA June 24, 2013 At 12:42
I agree Matt, as much as some black leaders are trying to make this about race, it just doesnt fit. I doubt we will see anything beyond peaceful demonstrations.
Black "leaders" are making this case about money. The "community" is not going to riot.
Will the blacks attack the Hispanics? Will the Hispanics attack the blacks if he is found guilty (which would be a miscarriage of justice)?
Tim McNabb June 24, 2013 At 10:36
It seems to me that the slide on a semi-auto pressed up against a soft surface could be pushed out of battery. After clearing my DB9, I checked to see how fr out of batter the slide could be and not function. Just under 1/4 inch.
Dryw June 24, 2013 At 10:44
Veracity of this factoid requires confirmation:
I have heard/read on more than one occasion that the forward protrusion on the guide rod on the HS2000 was a specific design feature to overcome this very problem (out-of-battery on contact shot).
As the HS is the predecessor to the XD (technically first gen XDs are HS2000s), and subsequently the XDM, the design remains intact in current production.
…which, is totally irrelevant to the point you were trying to make about the DB9.
Jeez… I need to double check context before I clickity-click.
The point is that the sweatshirt was hanging away from TM's body owing to his position on top of GZ, leaning out over him, when the pistol discharged. So, ONLY the loose hanging fabric came in contact with the pistol's muzzle. That should not knock it out of battery.
Cool stories, Bro's.
When did Neighborhood Watch protocol become law? Did I slip into a coma and miss something or is this lawyer just full of $hit?
We better get to see Trayvon's "character" as well.
They need to show those pictures of Trayvon doing those gangster smoke tricks:
http://i2.cdn.turner.com/cnn/dam/assets/130523170843-04-trayvon-0523-horizontal-gallery.jpg
I think that evidence of Trayvon's wannabe thug-life has already been excluded by the judge. Too hard to railroad Zimmerman if it were allowed. 😉
"Dont follow… and observe."
Anyone else catch that? Can you really do one without the other?
You can observe without following, sometimes. So no. It doesn't hold up the way you imagine it does.
True but at night how much can you see without following? I dont know how well lit his neighborhood is.
David June 24, 2013 At 11:11
I grew up in Orlando about 15 mins away from Sanford. I repoed all over FL. Sanford is a decent area to live, its between Lake Mary, which is upper class, and Altamont Springs, which is middle class. And THEN THERES 13th ST and 17-92…A REAL SHIT HOLE OF CRACK DEALERS, CRACK HEADS, and HOOKERS I repoed in this lovely area for over 18 years. And it sits right dab in the middle of Sanford. One of my repo agents got 3 9mm rounds threw his wrecker into his Kevlar vest, at 6pm in the daylight! This Trey fellow was watched pulling handles, a street code for checking to see if cars are unlocked. They have two different people that saw Trey doing this shortly before the shooting happened. If there had bin a crime actually committed, the Sherriff would have arrested Mark Z. That's why so many people are pissed off, the asshole media made this out to be a race crime, when in fact the only crime that was committed was assault on Mark Z. They have pulled all the BULLSHIT out of there lefty man purse on this one! I like how Obama said "this could have bin one of my kids" really, BULLSHIT!! Whens the last time his kids were in crack town???? Ohhhh and when were his kids seen trying to break into cars, when they should have bin in school, or home???? This whole case is polliticly motivated BULL SHIT, why do people outside our state need to get involved on a governmental level, im sure the state of FLORIDA can handle this without any FEDERAL HELP OBAMA!!
jwm June 24, 2013 At 12:19
Mark Z?
When it's made into a race issue, it becomes a race war issue. That's the fact. The globalists want race wars; they want a culture war. Anyone who can't fathom this is a dupe and a sheep-person.
Pitting racial and cultural groups against one another is part of their playbook. They pit black against white, rich against poor, privileged against unprivileged. It's the only way they can win.
Don't be a sucker and fall for it.
Y eah, state law gave him a CCW PERMIT, which overrides that BS community watch regs, there trying to say if your part of the citizen watch program you don't have the basic right of SELF DEFFENSE by KEEPING AND BARING ARMS!! Another attack on our Constitution!!! Makes me very mad to see this form of injustice!!
The people that believe a ccw permit gives them police powers probably shouldn't have the permit or the gun.
Shenandoah June 24, 2013 At 14:14
Can't tell if he's real or trolling…
scrumps June 24, 2013 At 16:55
Nothing but time and trolling on his or 'her' mind.
Bad.
"Repo" trolling- that's a cute one.
I don't believe anyone thinks that.
LongPurple June 24, 2013 At 15:10
My FL CCW came with a pamphlet that included the statement that having that permit did not make me "a free-lance police officer".
"Baring" is not even close to being a word. "Bearing" is the word you want.
Unless you meant "barring". I'm not going to explain that to you; look it up.
When you can't use your native language, you are, in effect, handicapped. You can look up "handicapped"; I recommend it.
Or do you advocate "keep(ing) and bar(ring) arms?
Rich Grise June 24, 2013 At 16:20
Uh, yes, "baring" is a word. It means, "making bare," i.e., exposing, or brandishing. But it's true, that's not what 2A says.
O.E June 24, 2013 At 21:27
Absolutely correct.
Changes nothing in regards to the Fed objectives of granting the Black koonmutiny what they want at cost of running rough shod over one mans legal correctness.
1 man in prison for a crime he did not commit ~ $1.5 million
thousands of square meters of property destroyed by rampaging black gangs ~ >$1.5 million
There are millions of white liberals out there shitting their pants & hoping the Z-man takes it for their team.
"You will know in your head, you will know in your heart and you will know in your stomach that George Zimmerman didn't shoot Trayvon Martin because he had to. He shot him for the worst of all reasons: because he wanted to."
Since we have no evidence – lets convict on speculation alone.
I believe the Attorney is engaging in "hear-say", i'm sure that was not made patently obvious to the jury at the time though.
"F"cking punks. These as"holes they always get away."
This is what I have always said about district attorneys.
" . . . because he wanted to."
If I had been sucker-punched, my nose broken, and the guy who did it was on top of me, pounding my head on the sidewalk, I just might "want to shoot him" before he kills me.
Yeah, but you'll never hide in the darkness alongside skid row waiting for drunken women to fall out of the nightclubs with their knickers around their ankles ever again.
HANG this "lawyer" and start fitting Zimmerman for a noose. Hispanics are soon to find out they, too, are meat for the grinder of the race and culture war the globalists have been fomenting since, well, nearly forever.
Andy June 24, 2013 At 20:28
This guy is going to be railroaded,if he is convicted!First off the pictures we see of Trayvon Martin are when he was younger,he had grown into a large set man,he worked out with weights,so he could be a better fighter in the fight club he belonged to,which to me shows a tendency toward violence.Next he was a drug user.It Appears that George Zimmerman is not a large man or wasn't when the altercation took place,in the state where I reside,Mississippi,the law reads that someone can defend themselves with a firearm even if the other person is not armed,due to the other persons age,physical stature,or the person being attacked being physically disabled,I don't know how Florida law is written but I bet it is close to the same.I think that it would be a travesty of Justice of George Zimmerman is convicted of killing what I alinjudge to be a lowlife thug,with a propensity toward violence.This is gonna be another Rodney King debacle if G.Z. Is exonerated,another sad day when we let the hoodlums run rampant in the streets looting and destroying property.Be prepared and ready.Keep your powder dry.
UK Government are adding fuel to the Black unrest fire.
http://www.guardian.co.uk/uk/2013/jun/24/stephen-lawrence-father-police-spy-inquiry
Mary lamb July 13, 2013 At 21:46
Prosecutors don't gloat cos u have just helped a criminal getaway with murder! Fact is-young boy followed by a fool with a gun,when told not to follow him,and decides to kill him! I hope Zimmerman never has a day of peace! U murderer! As a mum I wld make sure u rot in hell! Sad day for America!
Sorry,I meant to say 'the defence team'!
Prosecutors don't gloat cos u have just helped a criminal getaway with murder! Fact is-young boy followed by a fool with a gun,when told not to follow him,and decides to kill him! I hope Zimmerman never has a day of peace! U murderer! As a mum I wld make sure u rot in hell! Sad day for America! Sorry I meant to say defence team!


Additional Details on Murthy Legal Team's Victory in Matter of V-S-G- Inc.
As previously reported, the big news for employment-based immigrants is the adopted decision of Matter of V-S-G- Inc., recognizing that an employee waiting for the approval of the adjustment-of-status application (form I-485) has a recognized legal interest in the underlying I-140 petition. When Matter of V-S-G- Inc. was decided originally by the administrative appeals office (AAO), MurthyDotCom reported on our client's success in the NewsBrief, Murthy Success Story: AAO Recognizes Individual Rights in AC21 Case (15.May.2017). One of the interesting aspects at that time was that the AAO actually issued two decisions, one to the defunct petitioning employer and one to Adam Rosen, the attorney at the Murthy Law Firm who represents the foreign national beneficiary.
Background on Murthy Response in Matter of V-S-G- Inc.
The Murthy Law Firm has been representing the beneficiary in Matter of V-S-G- Inc. since the notice of intent to revoke (NOIR) originally was issued by the U.S. Citizenship and Immigration Services (USCIS), in July 2012. By that time, the beneficiary had already notified the USCIS that he had ported to a new employer under the American Competitiveness in the Twenty-First Century Act (AC21).
The Murthy Law Firm responded to the NOIR based largely on our legal theory that it was illogical to allow an I-140 beneficiary to port under AC21, but then prohibit that same person from defending the I-140 from revocation. The law upon which this logic was based came from the 2010 precedent decision of Matter of Marcal Neto.
The legal team at the Murthy Law Firm believed that Matter of Marcal Neto made sense because that decision, like Matter of V-S-G- Inc., involved a foreign national who had an approved I-140, an I-485 pending for 180 days, and a new job in the same-or-similar occupational category. Normally, immigration judges and the Board of Immigration Appeals (BIA) have no legal authority over I-140 related questions. The U.S. Department of Homeland Security (DHS) lawyers argued to the BIA that the "DHS does not view an INA § 204(j) [i.e. AC21] portability determination as a separate adjudication from the adjudication of the application for adjustment of status." Matter of Marcal Neto was issued with the BIA holding that a person in removal proceedings could defend his AC21 case even though it involved the I-140 petition. The reason, courtesy of the DHS, was that AC21 questions are all part of the I-485. So, if AC21 questions are just a subset of the I-485, then it should not matter whether the I-485 applicant is in removal proceedings, like in Matter of Marcal Neto, or not in removal proceedings, like our client.
In 2015, when the AAO asked for amicus curiae briefs from the public on the question of whether an I-140 beneficiary should be allowed to defend the petition against revocation, the Murthy legal team filed an amicus brief. We fought hard for our client's right to be heard by the USCIS and are thrilled that the AAO finally recognized the importance of giving a qualifying I-140 beneficiary the ability to defend the underlying I-140 petition from revocation.
The Murthy Law Firm is delighted to have won the case for our client on April 28, 2017. Just as important, it is gratifying to see that this decision has become an adopted decision for agency-wide policy, to benefit thousands of other employment-based immigrants.


Each box contains 8, ten foot sections of a great product "Fence Topper". Fence Topper Is an attractive polyethylene cap that fits over standard chain link fencing and when installed, fence topper creates a safe fence for your athletes and children as it is easily visible from a distance. Many states are now requiring day cares to cover the tops of chain-link fences to prevent injury to children.


Home / Dance / Welcome to the team Amy Hacking!
Welcome to the team Amy Hacking!
AVR is proud to announce another brand new teacher to the team!
'I Trained from the age of 3 to advanced 2 in ISTD modern and tap, ISTD jazz awards bronze – gold and passed grade 8 ballet with distinction. Credits whilst training include Evita musical tour and singing and dancing alongside Michael Ball.
At the age of 17 I started working professionally and I have worked in almost all of the venues offering shows and performances in Blackpool over the years. Various hotels, Blackpool tower ballroom and circus, talk of the coast, legends and was part of the opening show team for Viva.
International work includes working for Explosive Productions, UK summer tour for haven holidays and Fred Olsen cruise lines, The Braemar.
I spent 8 years on and off cruise ships dancing a variety of different styles and was very lucky to travel the world, American Caribbean, French Caribbean, Brazil and Argentina, Balearic islands, Norwegian fjords and the list goes on. Mostly my years on the ships were working for South African dance company, Afro Arimba onboard Costa Cruises.
Looking forward to starting work with you Amy!


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Q: Send all traffic from one IP address to another IP and port I have a UDM PRO 192.168.1.1 running ShadowSocks on 192.168.1.1:1080.
Here is what I am trying to do.

*

*I would to know the IPTABLES rules for a computer on my network 192.168.1.100 to route all traffic to the ShadowSocks Server. Eg. All traffic from my computer should only go to the ShadowSocks Server.

*I would like to send traffic on a particular port say 5036 to the ShadowSocks Server. This means that any device on this network that tries to use 5036 should get routed to the ShadowSocks server. Eg. Any device on my network using a particular app should be routed to the ShadowSocks Server.
I am trying to apply these rules to the UDM PRO IPTABELS via SSH.

redpajama_set_name: RedPajamaStackExchange


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Q: Cloudfront does not reliably store images over a period of days I have a webserver which is dynamically generating images for an e-commerce site, and I have set up Amazon Cloudfront in front of this server to cache image requests to reduce load on my own server.
I find that on any given day, images (once loaded a single time that day) are served from cloudfront. e.g:

The problem is that the image is being regenerated (Cloudfront is missing my image and needs to request it from my server) each day, cloudfront seems to ignore the 'max_age' I am defining on my own server and choosing a default of 24 hours. This is the response from the server 'behind' cloudfront:

Are there any reasons why cloudfront would be ignoring  my 'max-age' value based on the responses I'm sending? To add extra infuriation, it does hold on to some images for more than 24 hours, but never more than a couple of days.

A: This might be an artifact of how Amazon CloudFront operates regarding object expiration, see Specifying How Long Objects Stay in a CloudFront Edge Cache (Expiration):

Typically, CloudFront serves an object from an edge location until the
  object expires. After it expires, the next time the edge location gets
  an end-user request for the object, CloudFront forwards the request to
  the origin server to verify that the cache contains the latest version
  of the object:

*

*If CloudFront already has the latest version, the origin returns only a 304 status code (not modified).

*If CloudFront does not have the latest version, the origin returns a 200 status code (OK) and the latest version of the object.
If an object in an edge location isn't frequently requested,
  CloudFront might evict the object—remove the object before its
  expiration date—to make room for objects that are more popular. [emphasis mine]

Infrequent object access would also explain why it does hold on to some images for more than 24 hours, but never more than a couple of days. This should be evident by the lack of entries in the CloudFront Access Logs, so in case you aren't positive that those files are frequently requested, checking the logs could yield a definite answer.


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text:
Area Planning – Working Together on Shared Priorities
This month we begin with the first of a series of articles from Cumbria County Council providing updates on the 'area planning' work taking place in some of the main towns in Cumbria. Area planning is all about joining things up in the local area and improving the services people can access in their communities, and so it's likely that many third sector organisations will be interested in getting involved. This month the focus is on Eden…..
By Nick Wright, Cumbria County Council
Cumbria County Council has a long tradition of working with communities. We have six well-established Local Area Committees made up of County Council Elected Members representing each local area.
We engage with residents in a wide range of ways. Local Members know their communities well and champion their aspirations. They utilise connections in their localities and our area-working approach includes a focus on supporting Members in this role to maximise their impact as community leaders.
Partnership working is critical to developing our understanding of place, and we are continuously seeking ways to enrich this understanding. The council often refers to this partnership approach as 'area planning' which, put simply, is the process of working together, using all of our resources, to jointly deliver on shared priorities. It seeks to consider, in a holistic way, how such activity can bring about benefits whilst considering and carefully managing any direct and indirect consequences.
What's happening in Eden?
Current area planning priorities in Eden:
Developing strong and resilient communities
Supporting a better quality of jobs in the area
In Eden, the area planning process is starting with the district's largest town and also it's most remote – Penrith and Alston.
Penrith is the largest and most frequented town within Eden. With a population of over 15,000 it has the largest concentration of residents; it's the employment hub and often the first port of call for tourists visiting the wider district. As the main service centre for a district which already has 26% of its residents over the age of 65, a large proportion of council services are delivered from the town – 60% of all Eden's regulated community based adult social support services are delivered in Penrith. So a thriving Penrith, supports the entire district.
Current action in Penrith…
The County Council, District Council and Town Council along with the town's Chamber of Trade and both Business Improvement Districts have formed a working group to drive forward local initiatives.
The group are planning on performing a series of place audits around the town to determine some quick visual wins and longer term priorities.
Arrangements are being finalised to commission a study investigating issues and improvements with regards parking and movement around the town.
The Integrated Care Community Hub based at Penrith Hospital continues to grow with a Third-Sector Referral Co-ordinator, Jayne Povey, who started at the end of June. You can email her on: jayne.povey@cumbriacvs.org.uk.
While we're piloting a method of co-ordinating priority-based area working in Penrith, there is plenty going on in our second priority area of Alston. At 1000ft above sea level, and the highest market town in England, Alston is among the most remote parts of Eden. However, with one social enterprise per 50 households – it has addressed this by developing a culture of delivering local solutions. The key to successful area planning in the town is combining this energy with statutory and third sector resources.
Current action in Alston…
Plans are being considered around developing an expression of interest to take over the old primary school to create a multi-purpose community asset.
The steering group that is considering proposals around Front Street is engaging with residents over investigatory work for the early phases of the project.
The new Community Transport Group is developing new ways of improving movement options from the town.
There has been a community asset transfer of Nenthead mine.
Where will we be going next?
Area planning is a rolling programme of highlighting and seeking to achieve local opportunities. While Penrith and Alston will continue, we'll also look to build momentum around the process in Appleby and Kirkby Stephen. If you have suggestions for projects or ideas for developments for any of these towns, we'd like to hear from you. You can contact the Eden Community Development Team on…
Penrith and Alston
Tracey Moran: tracey.moran@cumbria.gov.uk
Tel: 01768 812662 / 0781 4481177
Appleby and Kirkby Stephen
Kellie Bradburn-Sims: kellie.bradburn-sims@cumbria.gov.uk


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text:
// Licensed to the .NET Foundation under one or more agreements.
// The .NET Foundation licenses this file to you under the MIT license.
// See the LICENSE file in the project root for more information.

//
/*=============================================================================
**
**
**
** Purpose: Base class for representing Events
**
**
=============================================================================*/

#if !FEATURE_MACL
namespace System.Security.AccessControl
{
    public class EventWaitHandleSecurity
    {
    }
    public enum EventWaitHandleRights
    {
    }
}
#endif

namespace System.Threading
{
    using System;
    using System.Threading;
    using System.Runtime.CompilerServices;
    using System.Security.Permissions;
    using System.IO;
    using Microsoft.Win32;
    using Microsoft.Win32.SafeHandles;
    using System.Runtime.InteropServices;
    using System.Runtime.Versioning;
    using System.Security.AccessControl;
    using System.Diagnostics.Contracts;

    [HostProtection(Synchronization=true, ExternalThreading=true)]
    [ComVisibleAttribute(true)]
    public class EventWaitHandle : WaitHandle
    {
        [System.Security.SecuritySafeCritical]  // auto-generated
        public EventWaitHandle(bool initialState, EventResetMode mode) : this(initialState,mode,null) { }

        [System.Security.SecurityCritical]  // auto-generated_required
        public EventWaitHandle(bool initialState, EventResetMode mode, string name)
        {
            if(name != null)
            {
#if PLATFORM_UNIX
                throw new PlatformNotSupportedException(Environment.GetResourceString("PlatformNotSupported_NamedSynchronizationPrimitives"));
#else
                if (System.IO.Path.MaxPath < name.Length)
                {
                    throw new ArgumentException(Environment.GetResourceString("Argument_WaitHandleNameTooLong", Path.MaxPath), nameof(name));
                }
#endif
            }
            Contract.EndContractBlock();

            SafeWaitHandle _handle = null;
            switch(mode)
            {
                case EventResetMode.ManualReset:
                    _handle = Win32Native.CreateEvent(null, true, initialState, name);
                    break;
                case EventResetMode.AutoReset:
                    _handle = Win32Native.CreateEvent(null, false, initialState, name);
                    break;

                default:
                    throw new ArgumentException(Environment.GetResourceString("Argument_InvalidFlag",name));
            };

            if (_handle.IsInvalid)
            {
                int errorCode = Marshal.GetLastWin32Error();

                _handle.SetHandleAsInvalid();
                if(null != name && 0 != name.Length && Win32Native.ERROR_INVALID_HANDLE == errorCode)
                    throw new WaitHandleCannotBeOpenedException(Environment.GetResourceString("Threading.WaitHandleCannotBeOpenedException_InvalidHandle",name));

                __Error.WinIOError(errorCode, name);
            }
            SetHandleInternal(_handle);
        }

        [System.Security.SecurityCritical]  // auto-generated_required
        public EventWaitHandle(bool initialState, EventResetMode mode, string name, out bool createdNew)
            : this(initialState, mode, name, out createdNew, null)
        {
        }

        [System.Security.SecurityCritical]  // auto-generated_required
        public unsafe EventWaitHandle(bool initialState, EventResetMode mode, string name, out bool createdNew, EventWaitHandleSecurity eventSecurity)
        {
            if(name != null)
            {
#if PLATFORM_UNIX
                throw new PlatformNotSupportedException(Environment.GetResourceString("PlatformNotSupported_NamedSynchronizationPrimitives"));
#else
                if (System.IO.Path.MaxPath < name.Length)
                {
                    throw new ArgumentException(Environment.GetResourceString("Argument_WaitHandleNameTooLong", Path.MaxPath), nameof(name));
                }
#endif
            }
            Contract.EndContractBlock();
            Win32Native.SECURITY_ATTRIBUTES secAttrs = null;
#if FEATURE_MACL
            // For ACL's, get the security descriptor from the EventWaitHandleSecurity.
            if (eventSecurity != null) {
                secAttrs = new Win32Native.SECURITY_ATTRIBUTES();
                secAttrs.nLength = (int)Marshal.SizeOf(secAttrs);

                byte[] sd = eventSecurity.GetSecurityDescriptorBinaryForm();
                byte* pSecDescriptor = stackalloc byte[sd.Length];
                Buffer.Memcpy(pSecDescriptor, 0, sd, 0, sd.Length);
                secAttrs.pSecurityDescriptor = pSecDescriptor;
            }
#endif

            SafeWaitHandle _handle = null;
            Boolean isManualReset;
            switch(mode)
            {
                case EventResetMode.ManualReset:
                    isManualReset = true;
                    break;
                case EventResetMode.AutoReset:
                    isManualReset = false;
                    break;

                default:
                    throw new ArgumentException(Environment.GetResourceString("Argument_InvalidFlag",name));
            };

            _handle = Win32Native.CreateEvent(secAttrs, isManualReset, initialState, name);
            int errorCode = Marshal.GetLastWin32Error();

            if (_handle.IsInvalid)
            {

                _handle.SetHandleAsInvalid();
                if(null != name && 0 != name.Length && Win32Native.ERROR_INVALID_HANDLE == errorCode)
                    throw new WaitHandleCannotBeOpenedException(Environment.GetResourceString("Threading.WaitHandleCannotBeOpenedException_InvalidHandle",name));

                __Error.WinIOError(errorCode, name);
            }
            createdNew = errorCode != Win32Native.ERROR_ALREADY_EXISTS;
            SetHandleInternal(_handle);
        }

        [System.Security.SecurityCritical]  // auto-generated
        private EventWaitHandle(SafeWaitHandle handle)
        {
            SetHandleInternal(handle);
        }

        [System.Security.SecurityCritical]  // auto-generated_required
        public static EventWaitHandle OpenExisting(string name)
        {
#if !FEATURE_MACL
            return OpenExisting(name, (EventWaitHandleRights)0);
#else
            return OpenExisting(name, EventWaitHandleRights.Modify | EventWaitHandleRights.Synchronize);
#endif
        }

        [System.Security.SecurityCritical]  // auto-generated_required
        public static EventWaitHandle OpenExisting(string name, EventWaitHandleRights rights)
        {
            EventWaitHandle result;
            switch (OpenExistingWorker(name, rights, out result))
            {
                case OpenExistingResult.NameNotFound:
                    throw new WaitHandleCannotBeOpenedException();

                case OpenExistingResult.NameInvalid:
                    throw new WaitHandleCannotBeOpenedException(Environment.GetResourceString("Threading.WaitHandleCannotBeOpenedException_InvalidHandle", name));

                case OpenExistingResult.PathNotFound:
                    __Error.WinIOError(Win32Native.ERROR_PATH_NOT_FOUND, "");
                    return result; //never executes

                default:
                    return result;
            }
        }

        [System.Security.SecurityCritical]  // auto-generated_required
        public static bool TryOpenExisting(string name, out EventWaitHandle result)
        {
#if !FEATURE_MACL
            return OpenExistingWorker(name, (EventWaitHandleRights)0, out result) == OpenExistingResult.Success;
#else
            return OpenExistingWorker(name, EventWaitHandleRights.Modify | EventWaitHandleRights.Synchronize, out result) == OpenExistingResult.Success;
#endif
        }

        [System.Security.SecurityCritical]  // auto-generated_required
        public static bool TryOpenExisting(string name, EventWaitHandleRights rights, out EventWaitHandle result)
        {
            return OpenExistingWorker(name, rights, out result) == OpenExistingResult.Success;
        }

        [System.Security.SecurityCritical]  // auto-generated_required
        private static OpenExistingResult OpenExistingWorker(string name, EventWaitHandleRights rights, out EventWaitHandle result)
        {
#if PLATFORM_UNIX
            throw new PlatformNotSupportedException(Environment.GetResourceString("PlatformNotSupported_NamedSynchronizationPrimitives"));
#else
            if (name == null)
            {
                throw new ArgumentNullException(nameof(name), Environment.GetResourceString("ArgumentNull_WithParamName"));
            }

            if(name.Length  == 0)
            {
                throw new ArgumentException(Environment.GetResourceString("Argument_EmptyName"), nameof(name));
            }

            if(null != name && System.IO.Path.MaxPath < name.Length)
            {
                throw new ArgumentException(Environment.GetResourceString("Argument_WaitHandleNameTooLong", Path.MaxPath), nameof(name));
            }

            Contract.EndContractBlock();

            result = null;

#if FEATURE_MACL
            SafeWaitHandle myHandle = Win32Native.OpenEvent((int) rights, false, name);
#else
            SafeWaitHandle myHandle = Win32Native.OpenEvent(Win32Native.EVENT_MODIFY_STATE | Win32Native.SYNCHRONIZE, false, name);
#endif

            if (myHandle.IsInvalid)
            {
                int errorCode = Marshal.GetLastWin32Error();

                if(Win32Native.ERROR_FILE_NOT_FOUND == errorCode || Win32Native.ERROR_INVALID_NAME == errorCode)
                    return OpenExistingResult.NameNotFound;
                if (Win32Native.ERROR_PATH_NOT_FOUND == errorCode)
                    return OpenExistingResult.PathNotFound;
                if(null != name && 0 != name.Length && Win32Native.ERROR_INVALID_HANDLE == errorCode)
                    return OpenExistingResult.NameInvalid;
                //this is for passed through Win32Native Errors
                __Error.WinIOError(errorCode,"");
            }
            result = new EventWaitHandle(myHandle);
            return OpenExistingResult.Success;
#endif
        }
        [System.Security.SecuritySafeCritical]  // auto-generated
        public bool Reset()
        {
            bool res = Win32Native.ResetEvent(safeWaitHandle);
            if (!res)
                __Error.WinIOError();
            return res;
        }
        [System.Security.SecuritySafeCritical]  // auto-generated
        public bool Set()
        {
            bool res = Win32Native.SetEvent(safeWaitHandle);

            if (!res)
                __Error.WinIOError();

            return res;
        }

#if FEATURE_MACL
        [System.Security.SecuritySafeCritical]  // auto-generated
        public EventWaitHandleSecurity GetAccessControl()
        {
            return new EventWaitHandleSecurity(safeWaitHandle, AccessControlSections.Access | AccessControlSections.Owner | AccessControlSections.Group);
        }

        [System.Security.SecuritySafeCritical]  // auto-generated
        public void SetAccessControl(EventWaitHandleSecurity eventSecurity)
        {
            if (eventSecurity == null)
                throw new ArgumentNullException(nameof(eventSecurity));
            Contract.EndContractBlock();

            eventSecurity.Persist(safeWaitHandle);
        }
#endif
    }
}

redpajama_set_name: RedPajamaGithub


Heaves is a common respiratory disease in equine patients. It can be characterized by chronic coughing, respiratory difficulty, and nasal discharge. Heaves is caused by mold or dust in hay, and once the respiratory system has been damaged heaves can only be managed, not cured.


The original boy-robot with 100,000 horsepower strength and courage! In the year 2000, Dr. Boyton creates a super-robot in his deceased son's image. He calls the robot Astro Boy. Astro Boy can swim oceans, leap over mountains, even fly into space on his own power. However, Astro Boy can't replace his son; Dr. Boyton becomes dissatisfied with the boy robot and disowns him. Astro Boy is befriended by Dr. Packadermus J. Elefun of the Institute of Science, who guides him through his adventures. Endowed with super strength, rocket-powered flight, a selfless heart and a kind demeanor, Astro Boy fights a never-ending crusade against the forces of evil!


text:
All authors read and approved the final manuscript "
"Backgr
Posted on June 30, 2019 by admin2
All authors read and approved the final manuscript."
"Background Botrytis cinerea is a pathogen ascomycete, which causes gray mold on a large number of economically important agricultural and horticultural crops [1–4]. This ubiquitous fungal pathogen is present often as latent infection. Latency is generally defined as the period between infection and the appearance AZD9291 of visible symptoms and can in the case of B. cinerea be long and variable [5–8]. Consequently, an apparently healthy fruit can deteriorate suddenly due to the development of this latent infection [9, 10]. Many synthetic fungicides are used as the principal mean of controlling
this important postharvest disease [11]. However, the growing public concern over the health and environmental hazards associated with fungicide use in orchards, the development of fungicide resistant strains of B. cinerea [12], and the deregistration of some of the most effective fungicides [13], have generated a great interest in the development of alternative methods to control the postharvest disease caused by this fungal pathogen. To prevent the indiscriminate use of fungicides, a sensitive and reliable method to early determination of the fungus in fruit tissues becomes crucial. The ability to detect latent infections in fruit
tissues should prove useful not only for early disease management but also for identifying infected fruit in postharvest. In addition, the quantification of the pathogen is necessary for the application of alternative methods of control, such as biological control using antagonist microorganisms because the success Ureohydrolase AR-13324 datasheet of this method depend of the ratio antagonist/pathogen [14]. The detection of fungus in fruit includes classical methods such as isolation on selective media, which is useful but subject to limitations [15] due to many pathogens can be masked by overgrowth of faster growing fungi. Other methods, such as quantitative real-time polymerase chain reaction (Q-PCR), or reverse transcription
polymerase chain reaction (RT-PCR) represent new tools for the detection of the pathogens by determination of their DNA/RNA [16–25]. Unfortunately these methods are expensive and not easy to perform routinely, because they require highly qualified personnel and need sophisticated instrumentation [26, 27]. In addition, to methods mentioned previously, some direct enzyme-linked immunosorbent assays (ELISAs) using microtiter plates have been developed for the detection of B. cinerea in pear steam, grape juice, and plants [28–32], but at present has not been reported any validated method based in an indirect competitive immunoassay for detection and quantification of the mentioned fungus in tissues of fruits. The aim of this study was the development and corroboration of a sensitive and specific ELISA for B.
2, red circles and Additional File 5, Table S5) Of these 82 stat
2, red circles and Additional File 5, Table S5). Of these 82 statistically significant altered transcripts, only 4 were commonly altered with the same magnitude by a deletion of vjbR or wildtype cells treated with C12-HSL (Fig. 2). At the exponential growth phase, administration of C12-HSL exerted an equal effect on gene expression, up and down-regulating
19 and find more 23 genes (respectively, Fig. 2). On the contrary, at the stationary phase all 48 genes were up-regulated, a dramatically different profile than the down-regulation observed for the majority of differently expressed genes in C12-HSL treated wildtype cells (Fig. 2). Collectively, this data supports that C12-HSL is capable of influencing gene expression independent of VjbR. There is evidence that C12-HSL may interact with a second LuxR homologue, BlxR [18]. Induction of blxR expression in response to C12-HSL was highly variable by microarray analysis; however, qRT-PCR revealed that blxR was up-regulated 99.5-fold in bacteria lacking HSP inhibitor drugs vjbR treated with C12-HSL, compared to 27.5-fold in wildtype cells that were administered C12-HSL at the stationary growth phase. One possible explanation for this observation is that VjbR inhibits the induction of blxR by binding the AHL substrate and therefore
lowering the cellular concentration of available C12-HSL for blxR induction, but has not been demonstrated. Interestingly, 58% of the gene transcripts found to be altered in an recent study of the function of ΔblxR were also found to be altered by the addition of C12-HSL in the ΔvjbR background, and increased to 88% if we lowered the threshold from our 1.5-fold cutoff (Additional File 5, Table S5) [15]. A second study that similarly examined the transcript and proteomic alterations due to a deletion in babR corresponded with 6 genes identified in our study: with 2 genes found to be unique to the addition of C12-HSL in the ΔvjbR background (BMEI0231 and I1638, Additional File 5, Table S5), and 4 genes additionally altered by the deletion of vjbR or addition
of C12-HSL in the wildtype background (BMEI0451, I0712, I1196 and II0358, Additional File 3, Table S3) [23]. Although Cyclin-dependent kinase 3 many of these genes were not statistically significant in our analyses, this is a strikingly high correlation since the same conditions were not examined (ΔblxR vs. wt compared to ΔvjbR vs. ΔvjbR + C12-HSL), as well as the use of differing microarray platforms and analyses procedures. This connection may suggest that the genes altered by the presence of C12-HSL in the absence of VjbR may be due to C12-HSL activation of BlxR. Conclusions The goal of this work was to provide an elementary understanding in the role of the putative QS components in the virulence and survival of B. melitensis.
Anti-tumor effect of CIK plus L-OHP in the human drug-resistant g
Anti-tumor effect of CIK plus L-OHP in the human drug-resistant gastric cancer cellular peritoneal transplantation model Tumor weight and abdominal circumference were measured 21 days postinoculation (i.e., 7 days after intraperitoneal administration). The mice were sacrificed, and the number of ascites was calculated. The criterion for being cured was 60-day survival after inoculation with tumor cells. Pathomorphological observations in the human drug-resistant gastric cancer cellular peritoneal transplantation model after the treatment of L-OHP and CIK cells Tissue
sections were acquired 24 h after final injection in each group, and macroscopic observation was used to detect changes of P005091 chemical structure peritoneal transplantation nodules. The transplantation nodules in the omentum majus of each mouse were selected and divided into two sections, which were then used for routine pathological sectioning and transmission
electron microscope examination. Statistical analyses All data are expressed as mean ± SD, and buy CAL-101 analyses were carried out using SPSS 12.0 software (SPSS Inc, Chicago, IL). One-way analyses of variance (ANOVA), homogeneity tests for variance and Student's t-tests were used for comparisons of means. A p-value less than 0.05 was considered statistically significant. Results Cell biological characteristics of OCUM-2MD3/L-OHP cells Morphological observations of drug-resistant cells As is shown in Fig.1A and 1B, the two cell types in suspension appeared round under an inverted phase contrast microscope. Following cell adhesion, cells appeared spindle-shaped, were arranged in a single layer of different sizes, and showed no significant difference in cell morphology. The microvilli on the surface of parental cells were quite abundant under a transmission electron
microscope, and the morphology of organelles in the cytoplasm was normal. The nuclei of the cells appeared abnormally large and were irregularly shaped. Moreover, euchromatin was abundant, heterochromatin was limited, and the nucleolus was large and clearly visible (Fig. 1C). There was no significant difference in morphology of drug-resistant cells compared with OCUM-2MD3 cells. (Fig. 1D). Figure 1 A. OCUM-2MD3 cell (Phase contrast microscope × 400); B. OCUM-2MD3/L-OHP L-NAME HCl cell (Phase contrast microscope × 400); C. OCUM-2MD3 cell (TEM × 5000); D. OCUM-2MD3/L-OHP cell (TEM × 5000). Growth curve and population doubling time of drug-resistant cells As shown in Fig. 2, proliferation speed of drug-resistant cells was slower than that of parental cells. The population doubling time of drug-resistant cells was 27.0 ± 2.04 h by cell counts, which extended for approximately 3 h (P < 0.05). Figure 2 Cell-growth curve of OCUM-2MD3/L-OHP. Cell cycle distribution and apoptosis of drug-resistant cells As shown in Table 1, Fig. 3 and Fig.
It has also been shown that spermine can reduce the inflammatory
It has also been shown that spermine can reduce the inflammatory response by post-transcriptional inhibition of the production of pro-inflammatory cytokines, including TNFα, IL6, MIP-1α, and MIP-1β [19], and even though IL-8 was not included in this study, it is possible that it is regulated by spermine as well. Thus, in the interaction of wild type H. pylori with AGS cells, spermine levels may be elevated in the AGS cells, leading to a dampening of the chemokine/cytokine pro-inflammatory response. These possibilities await selleck screening library further in depth analyses. We performed pair-wise comparison of transcriptome on
the human adenocarcinoma CA4P manufacturer gastric cell line AGS after infection with 26695 wild type, its isogenic rocF- knockout mutant, and a rocF- complemented (rocF+) H. pylori strain, with uninfected AGS cells as a control. The first observation with the microarray analysis was an overall increase in the number of genes that participate in several signaling pathways previously investigated with H. pylori infection, notably with NFKB and AP-1 activation and mitogen-activated protein
kinase (especially ERKs, JNKs, SAPKs) [20], along with JUN-mediated signaling. From this activation cascade, the induction of IL-8 marked the greatest difference between the rocF- mutant H. pylori versus either the WT or the rocF + complemented strain. Our results show
a significant increase of mRNA and protein levels of IL-8 in AGS cells infected with the rocF- mutant strain, suggesting that WT bacteria may be able to control the inflammatory infiltration of immune cells by controlling the production of IL-8, which is a potent chemotactic factor for inflammatory cells, especially neutrophils [21–24]. While many H. pylori factors have been suggested to stimulate IL-8 expression, including peptidoglycan, LPS, CagA, VacA, PicB, IceA, urease (and even ammonia) [25–28], less is known about bacterial factors involved in suppression of cytokine production, especially in epithelial cells. Mechanisms for immune 17-DMAG (Alvespimycin) HCl evasion by H. pylori have been demonstrated, including the presence of a less potent LPS and cholesterol glycosylation [29]; however, fewer studies dealt with reduced host cytokine production as an immune suppressive mechanism, including effects on IL-12 [30–32]. While an increased amount of cytokines can result in histologically more intense gastritis [33], the limitation of this cytokine induction could be an advantage to the bacteria so that it can stay under the radar of the immune system. However, due to the complexity of the H.
Previously, Sedgwick et al [1] reported that the Ada regulon cou
Previously, Sedgwick et al. [1] reported that the Ada regulon could be induced
CA3 during stationary phase and could protect against active alkylators produced by nitrosation of amino acids in non-growing cells. Therefore, an increase in expression of the adaptive response genes, in parallel with expression of the genes producing active alkylators during the stationary phase prevents alkylation damage to DNA and subsequent mutagenesis. Transcriptome and proteome profiles of the ada mutant strain in response to MMS The transcriptional and translational responses of the ada mutant strain to alkylation stress were vastly different from those observed in W3110 strain (Figure 2). In the ada mutant strain, the expression levels of many more genes were significantly changed at 0.5 h after MMS treatment; 932 genes were up-regulated, which was about seven-fold more than that observed in the wild-type strain. Also, 12 genes of known function were down-regulated (Figure 2). The responses of the ada mutant to alkylating agents revealed several common themes, including the activation of genes involved in the transport of ions, sugars and amino acids and in detoxification processes (Figure 4).
This result indicates that the ada mutant cells induce various genes related to influx or efflux of solutes as a means of preventing and repairing alkylation damage. However, unlike the wild-type cells, in which these genes were up-regulated at 3.9 h after MMS treatment, the CX-5461 in vivo expression of transport genes was down-regulated in the ada mutant cells after the initial alkylation
stress was compensated. Based on these results, it can be assumed that the transport system substitutes for the adaptive response system in the ada mutant strain to coordinate the instant activation of the cellular repair systems after MMS treatment. More details are described below. Figure 4 Schematic diagram of up-regulated genes in the MMS-treated E. coli ada mutant strain. The two-component system related to drug or antibiotic resistance and the operons of genes related to respiration and transport are shown. The genes up-regulated more than 2-fold by 0.5 h MMS treatment, based Ribonucleotide reductase on the corresponding untreated control in the ada mutant strain, are indicated in black bold type. Proteome analysis showed variations in the production levels of 21 protein spots; the spot intensities of 18 proteins increased while 3 proteins decreased (Figure 3, Additional file 1: Table S1). Consistent with the transcriptome data, the intensities of proteins involved in metabolism and transport were increased. Proteins that showed significantly increased spot intensities in MMS-treated ada mutant cells at 0.
In this study we administered tylosin at therapeutic doses to hea
In this study we administered tylosin at therapeutic doses to healthy dogs with a pre-existing jejunal fistula and analyzed changes in bacterial communities before, during, and 14 days after cessation of tylosin by 16S rRNA gene pyrosequencing. Our results indicate a previously uncharacterized high species richness in the canine jejunum. Tylosin had a profound effect on the microbial selleck kinase inhibitor composition in the small intestine of dogs. Furthermore, tylosin had also a pervasive effect on specific bacterial taxa, which failed to recover within 14 days. However, these changes were not associated with any short-term clinical signs of gastrointestinal disease in healthy dogs.
Our results illustrate the complexity of
the intestinal microbiota and the challenges associated with evaluating the effect of antibiotic administration on the various bacterial groups and their potential interactions. The results also suggest that the proposed mode of action of an antibiotic on different bacterial genera does not necessarily match the in vivo effects, as several bacterial groups that are considered to be sensitive to tylosin increased in their proportions. Results Animals All dogs tolerated the course of antibiotics well and no obvious side effects (e.g., clinical signs of gastrointestinal disease such as diarrhea) were ABT-263 concentration noted during the study period. The body weights or body condition scores of the dogs did not change during the study. Characterization of the canine small intestinal microbiota A total of 44,069 pyrosequencing GBA3 tags were evaluated across all 15 samples (mean ± SD: 3188 ± 1091 sequencing tags per sample). All dogs showed highly diverse microbial communities within their small intestine. Table 1 lists the mean number of obtained and maximum predicted OTUs and richness estimators at strain (1% dissimilarity), species (3%), and genus (5%) level [19]. At day 0 and at 3% dissimilarity, which is commonly used to describe the species level [19], a range of 25-453 OTUs (mean: 218 OTUs) was observed,
indicating strong inter-individual differences in microbial diversity in the canine jejunum. The Chao 1 and Ace richness estimators were used to estimate the total number of OTUs in the canine jejunum. On day 0 and at 3% dissimilarity, the Chao 1 estimated between 32 and 707 OTUs (mean: 342 OTUs) per sample, and the Ace estimated between 32 and 721 OTUs (mean: 332 OTUs) per sample. To estimate the maximum number of OTUs at various dissimilarities, a Richards equation was fit to the obtained rarefaction curves [20]. Table 1 shows the mean number of maximum predicted OTUs in the canine jejunum: on day 0 (begin of the study) and at 3% dissimilarity (species level), the maximum predicted number of OTUs ranged from 32 to 666 (mean: 293 OTUs). At 1% dissimilarity (strain level), a mean of 950 OTUs (range: 183 to 1,789) was predicted.
selective thymidylate kinase inhibitors have
selective thymidylate kinase inhibitors have been developed and showed potent inhibitory effect in vivo against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus[22, 23]. Toxicity or side effect of these thymidylate kinase inhibitors to humans remains to be seen. Mycoplasmas, in general, depend on exogenous supply of precursors for their nucleotide biosynthesis because they lack the de novo synthesis of purine and pyrimidine bases. Nucleosides and deoxynucleosides are efficiently taken up and phosphorylated to their respective nucleotides by deoxynucleoside kinases such as thymidine kinase (TK) and deoxyadenosine kinase. Nucleobases are salvaged through hypoxanthine guanine phosphoribosyltransferase (HPRT), adenine phosphoribosyltransferase R406 (APRT) and uracil phosphoribosyltransferase (UPRT) systems [24–32]. P5091 research buy Of a total of 17 enzymes in nucleotide biosynthesis identified in the Mpn genome, 15 are essential. Enzymes mentioned above, TK, HPRT, APRT and UPRT are essential for Mpn survival while thymidylate synthase (TS), an enzyme catalyses the reductive synthesis of thymidylate from uridylate, is not since thyA mutant Mpn strain that lacks TS is viable [31, 33, 34]. In this study, 30 FDA-approved nucleoside and nucleobase analogs that
are anticancer or antiviral drugs were screened for inhibitory effects on Mpn growth. Seven analogs showed potent inhibitory effects on Mpn growth at clinically achievable plasma concentrations.
Among Nutlin-3 them, 6-thioguanine (6-GT) inhibited Mpn growth with a MIC (minimum inhibitory concentration required to cause 90% of growth inhibition) value of 0.20 μg ml-1. To investigate the mechanism of action of these drugs, we studied the effects of these analogs on uptake and metabolism of natural nucleoside and nucleobases by using tritium labelled natural substrates. Furthermore Mpn hypoxanthine guanine phosphoribosyl transferase (HPRT) was cloned and expressed, and the recombinant enzyme was purified and characterized using tritium labelled hypoxanthine and guanine as substrates, and 6-thiuoguanine and other purine analogs as inhibitors. The role of thymidine kinase in the inhibitory effect of trifluorothymidine against Mpn growth was also investigated. Results Inhibition of Mpn growth by nucleoside and nucleobase analogs Some nucleoside analogs have been reported to inhibit Mycoplasma growth [30, 35]. Recently a nucleoside and nucleobase analog library consisting of FDA-approved prodrugs used in anticancer and antiviral therapy was used to screen human enzymes in nucleotide metabolism, and new interactions were found [36], which promoted the use of these analogs in screening for inhibitory effects on Mpn growth.
, Carlsbad, CA, USA) and Oligo(dT) primer Primer sequences, gene
, Carlsbad, CA, USA) and Oligo(dT) primer. Primer sequences, generated using GenBank searches with BLASTN, were used to generate PCR products using Taq DNA polymerase (TaKaRa Ex Taq™ Takara Bio Inc., Kyoto, Japan) and an iCycler thermocycler (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Pilot studies were performed to determine the optimal annealing temperature and to confirm a linear correlation between the number of PCR cycles and the densitometric intensity of amplicons. Samples were analyzed for genomic
DNA contamination by PCR analysis of total RNA. PCR products were size-separated by electrophoresis on 2% agarose gel, NCT-501 mw visualized by ethidium bromide staining under UV light, and analyzed by scanning densitometry. Results were expressed as density of transgelin 2 in relation to β-actin, an internal control, expression within the same sample. Western blotting Western blot detection of transgelin 2 and the internal control β-actin, was performed using standard protocols. In detail, lung tissue specimens from all subjects
were homogenized to obtain protein extracts. The protein lysate was added to one-third volume of the SDS preparation buffer (NuPAGE 4× LDS Sample Buffer, Invitrogen Corp.). These protein samples (50 μg) were separated by 12.5% SDS-polyacrylamide gel electrophoresis. The proteins were then transferred electrophoretically to nitrocellulose membranes, which were incubated with a GM6001 solubility dmso mouse anti-transgelin 2 monoclonal antibody (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA). After secondary antibody application, immunodetection was performed by enhanced chemiluminescence on X-ray films (Fuji films). The mouse
anti-actin antibody (MAB 1501, Chemicon, Temecula, CA, USA) was used to normalize transgelin before 2 expression. Films were scanned and the protein lanes were quantified using Photoshop CS2 image analysis software (Adobe Systems Inc., San Jose, CA, USA). Results Characteristics of the three nanomaterials The size and shape of nanoparticles were summarized in Figure 1 (1-1). Our characterizations indicated that SiO2 nanoparticles exhibited a crystal structure with an average size of 20.2 nm (Figure 1 (1-1A)), that Fe3O4 nanoparticles had a sphere shape with an average size of 40 nm (Figure 1 (1-1B)), and that CNTs were rope-shaped with lengths <5 μm and diameters of approximately 8 nm (Figure 1 (1-1C)). Each chemical composition was quantitatively analyzed using a Raman spectroscopic technique and showed a purity >99.0% for all three nanomaterials. Pathological observations of the lung Histopathological evaluation of lung tissues revealed that pulmonary exposures to nanoparticles in rats produced persistent and progressive lung inflammatory responses.
Eur Respir J 2013 doi:10 1183/09031936 00149212 erj01492–2012;
Eur Respir J 2013. doi:10.1183/09031936.00149212. erj01492–2012; published ahead of print 29. Dawson D: Potential pathogens among strains of mycobacteria isolated from house-dusts. Med J Aust 1971, 1:679–681.PubMed 30. Reznikov M, Leggo JH, Dawson DJ: Investigation by seroagglutination of strains of the Mycobacterium Alisertib in vivo intracellulare-M. scrofulaceum group from house dusts and sputum in Southeastern Queensland. Am Rev Respir Dis 1971, 104:951–953.PubMed 31. Tuffley RJ, Hollbeche D: Isolation of the mycobacterium avium-M. intracellulare-M. scrofulaceum
complex from tank water in Queensland, Australia. Appl Environ Microbiol 1980, 39:48–53.PubMed 32. McSwiggan DA, Collins CH: The isolation of M. kansasii and M. xenopi from water systems. Tubercle 1974, 55:291–297.PubMedCrossRef 33. September S, Brozel V, Venter S: Diversity of nontuberculoid mycobacterium species in biofilms https://www.selleckchem.com/products/sb273005.html of urban and semiurban drinking water
distribution systems. Appl Environ Microbiol 2004, 70:7571–7573.PubMedCrossRef 34. Van Ingen J, Boeree M, Dekhuijzen P, Van Soolingen D: Environmental sources of rapid growing nontuberculous mycobacteria causing disease in humans. Clin Micro Inf 2009, 15:888–892.CrossRef 35. Huang W-C, Chiou C-S, Chen J-H, Shen G-H: Molecular epidemiology of Mycobacterium abscessus in a subtropcal chronic ventilatory setting. J Med Micro 2010, 59:1203–1211.CrossRef 36. Pedley SBJ, Rees G, Dufour A, Cotruvo J: Pathogenic Mycobacteria in Water. London: IWA Publishing; 2004. 37. Feazel L, Baumgartner L, Peterson K, Frank D, Harris J, Pace N: Opportunistic Urease pathogens enriched in showerhead biofilms. PNAS 2009, 106:16393–16399.PubMedCrossRef Authors' contributions
RT designed the study, coordinated the collection of samples, participated in the processing of water samples, collated and analysed the data, and wrote the manuscript. RC coordinated, received and processed the water samples (including subculturing and sequencing), collated the results and reviewed the manuscript. CT processed water samples, performed sequencing and collated results.CC contributed to the study design, provided institutional support and reviewed the manuscript. FH intellectually contributed to the study design and methodology and the writing of the manuscript. MH intellectually contributed to the study design and methodology, liaised with Brisbane Water, and contributed to the writing of the manuscript. All authors read and approved the final manuscript."
"Background Legionella pneumophila is the major cause of sporadic cases and outbreaks of legionellosis (91.5%), with sero-group 1 being the predominant serotype (84.
wjes org/supplements/7/S1 References 1 Country Profiles: 2
wjes.org/supplements/7/S1. References 1. Country Profiles: 2009: Top 20 Countries in ALL FIELDS, 1999- August 31, 2009 Avaible at: http://sciencewatch.com/dr/cou/pdf/09decALL.pdf. Avaible at: . 2. Heldwein FL, Hartmann AA, Kalil AN, Neves BVD, Ratti GSB, Beber MC Jr, et al.: Cited Brazilian papers in general surgery between 1970 and
2009. Clinics 2010,65(5):521–529.PubMedCrossRef 3. Waiselfisz JJ: Captisol order Map of Violence 2011. The young people of Brazil. Brasília: Ministry of Justice 2009. 4. Reichenheim ME, Souza ER, Moraes CL, Jorge MHPM, Silva CMFP, Minaya MCS: Violence and injuries in Brazil: the effect, progress made, and challenges ahead. Lancet 2011, 377:1962–1975.PubMedCrossRef 5. Paim J, Travassos C, Almeida C, Bahia L, Macinko J: The Brazilian health system: history, advances, and challenges. Lancet 2011, 377:1778–1797.PubMedCrossRef 6. Victora GC, Barreto ML, Leal MC, Monteiro CA, Schmidt MI, Paim J, et al.: Health conditions and health-policy innovations in Brazil: the way forward. Lancet 2011, 377:2042–2053.PubMedCrossRef 7. Almeida-Filho A: Higher education and health care in Brazil. Lancet 2011, 377:1898–1900.PubMedCrossRef 8. Birolini D: Trauma: social and medical challenge. J Am Coll Surg 2008,207(1):1–6.PubMedCrossRef 9. Green SM: Trauma surgery: discipline in crisis. Ann Emerg Med 2009, 53:198–207.PubMedCrossRef 10. The Committee to Development the Reorganized Specialty of Trauma, Surgical Critical
Care, and Emergency Surgery: Acute Care Surgery: Trauma, Critical care, and Emergency Surgery. J Trauma 2005, 58:614–616.CrossRef 11. ISI Web of knowledge database Available
Selleckchem RXDX-101 at: http://apps.isiknowledge.com. Available at: . 12. Ministry of Health Department of Science and Technology, Ministry of Science, Technology and Strategic Inputs: Decentralization in the context of promoting health research. Rev. Saúde Pública 2011,45(3):626–630. 13. Marques F: Advances and challenges. Fapesp 2011, 185:26–33. 14. Berwanger O, Riberio RA, Finkelsztejn A, Watanabe M, Suzumura EA, Duncan BB, et al.: The quality of reporting DNA ligase of trial abstracts is suboptimal: Survey of major general medical journals. Journal of Clinical Epidemiology 2009, 62:387–392.PubMedCrossRef 15. Ciesla DJ, Moore EE, Moore JB, Johnson JL, Cothren CC, Burch JM: The Academic Trauma Center Is a Model for the Future Trauma and Acute Care Surgeon. J.Trauma 2005,58(4):657–662.PubMedCrossRef 16. Schimidt MI, Duncan BB, Silva GA, Menezes AN, Monteiro AC, Barreto SM, et al.: Chronic non-communicable diseases in Brazil: burden and current challenges. Lancet 2011, 377:1949–1961.CrossRef 17. Mello Jorge M, Koizumi M: Traffic accidents in Brazil: an atlas of their distribution. In São Paulo. ABRAMET; 2007. 18. Krug EG, Dahlberg LL, Mercy JA, Zwi AB, Lozano R: World report on violence and health. Geneva: World Health Organization; 2002. 19. WHO: Age-standardized mortality rates by cause (per 100 000 population). Geneva: World Health Organization; 2008. 20.


text:
Director Kimberly Peirce Dissects Carrie
The director deconstructs the elements that make a pivotal scene work, and explains what attracted her to this story.
By Elly Fishman
Seen on the scene: 1. The eerie house; 2. the overbearing mother; 3. the sheltered daughter; 4. the prom dress. Photo: Michael Gibson/2012 Metro-Goldwyn-Mayer Pictures Inc. and Screen Gems, Inc./Sony Pictures
This Friday, October 18, a new adaptation of Stephen King's 1974 horror novel, Carrie, hits theatres. It's already drawing comparisons to Brian de Palma's 1976 classic by the same name.
Fans of the original flick can expect more emphasis on Carrie's relationship with her mother, Margaret (Julianne Moore). Director Kimberly Peirce calls that the "heart and soul" of the story.
Here, she discusses the well-researched elements of an ominous scene between the two as Carrie (Chloë Grace Moretz) prepares for the prom.
1. The Eerie House
"The walls are beautifully worn down," says Peirce of the yellowed, dirt-covered panels created by set designer Carol Spier (Eastern Promises). "You would imagine they were painted 20 years ago."
2. The Overbearing Mother
"They're completely codependent," says Peirce of Margaret White (Julianne Moore) and her daughter. Margaret spends much of the film beating and verbally abusing Carrie, but here, as she lurks in the doorway, the tables have turned. "Carrie has become the woman of the house."
3. The Sheltered Daughter
To play up Carrie's isolation, the director encouraged Chloë Grace Moretz to untether herself from her family and creature comforts. She even went so far as to bring the 16-year-old to a homeless shelter to help her identify with some of Carrie's issues.
4. The Prom Dress
After looking at hundreds of dresses, Peirce finally landed on a simple design in a basic fabric. "It had to be aspirational and fancy, but we also wanted it to be something Carrie could have made herself."
A Q&A with Director Kimberly Peirce
Movie director Kimberly Peirce (Boys Don't Cry) doesn't shy away from tough topics such as sexuality, androgyny, and violence. So when the University of Chicago graduate signed up to remake the iconic horror film Carrie based on the book by Stephen King, she dove right in to the story's themes of bullying, female empowerment, and religious fundamentalism. Chicago sat down with Peirce to discuss her remake.
Carrie is a story that has already lived nine lives (see "Carrie Through the Years," below), why make this movie now?
Well, first, I read the book three times and fell madly in love with Carrie. She's an extraordinary character. She's a misfit with an incredibly strong need to find love and acceptance. I also thought this was an opportunity to tell a superhero origins story: With the period comes the power. In the beginning, Carrie doesn't know how big her powers can go, and that's something we could track. That's great because it's what you get in these super hero movies like Superman and Spiderman.
Stephen King presciently observed the issues of bullying, religious radicalism, and female empowerment in his 1974 novel. How did you turn those issues into entertainment in a post-Columbine era?
It's interesting; I've noticed there are a lot more of these types of stories, avenger and superhero type stories. In an era like this when there is far more violence in everyday life and culture, people must feel insecure, which must be why we really love superhero stories. Superheroes make us feel safer; they're a reflection of us because he or she is usually a bullied weakling who gets powers and seeks out justice. Maybe it's our sense of vulnerability, but it's a blast to see somebody get the power that we could like to have.
There are several classic American superhero comics written by Jews in the 1950s.
There are. That was a level of disempowerment. They wanted to be über powerful. Maybe in the 1970s you had less of this, but now, there's a lot more violence in our everyday life and culture and everyday entertainment.
Switching gears, can you talk about your decision to cast Chloë Grace Moretz?
One thing I really love about Chloë is that she has movie start quality. The camera loves looking at her. She's utterly entertaining; she's got a great voice and she's really charismatic. I also thought she was the right age because she is truly a teenager. And that meant she was going through a lot of things the real Carrie was going through. One challenge, however, was that Chloë is a child star and is used to a certain comfortable lifestyle that is very different from Carrie. In a lot of ways, Carrie and Chloë are opposites. But that's the magic of a great actor.
It's a big coup for the film to have Julianne Moore star as Carrie's extremely religious mother Margaret. Did you have to change the character in order to better reflect the current role of religious fundamentalism in American culture?
When Brian de Palma first cast this role, he was bringing religious fundamentalism into popular culture in ways people hadn't really seen before. And once he introduced it, people loved it, and it worked great [in the film]. Cut to 40 years later, religiosity has played a new role. It's much more front and center and it's much more in the media. If you have a character that's excessive and religious you run the risk of mocking that character. But with an actress like as gifted and amazing as Julianne Moore, we could work on conveying Margaret as an antagonist who the audience could identify with.
What was Moore's relationship like with Moretz on set?
Two things happened. They had a real mother, daughter relationship. Chloë is a real teenager and Julianne is a wonderful mother who really privileges family. So they bonded. The other thing was that as Chloë worked with Julianne, who is an extraordinary actor, she just got better and better; better actor makes better actor. We all felt it on set. We said we were lost in the White House [Carrie's house] during that time.
The house plays an important role in the movie. How did you design it and what did you want to convey?
We really wanted to differentiate the house from the rest of the outside. The house is a safe space for Margaret. She also beats Carrie, and she only beats her inside the house. That's why Carrie never wants to go inside. It's a very emotional movie and Carrie's powers erupt from emotion. The house feels the relationship between the women. We built in this wonderful soundscape that reacts to how the two women engage each other. It mourns, aches, and grieves with the women, and in the end, it dies with them.
Carrie Through the Years
Photography: (1976) Everett Collection, Inc.; (2002) Paul Michaud/NBC
1974: Stephen King's best-selling novel is one of the first books to take on bullying and religious radicalism.
1976: Brian De Palma's blood-soaked movie premieres to critical and commercial acclaim; two Oscar nominations later, it's a Halloween classic.
1988: An $8 million musical adaptation becomes one of the most expensive flops in Broadway history.
1999: The Rage: Carrie 2 hits movie theatres. Don't remember it? Neither does anyone else.
2002: Writer Bryan Fuller (Dead Like Me, Heroes) pens a two-hour NBC special with a PG rating. Nuff said.
2012: Off Broadway takes a stab— this time the musical survives 34 previews and 46 shows.
2013: Kim Peirce releases her remake. She will discuss the film at the Humanities Festival on November 9. For info, chicagohumanities.org
Tags: Arts & Culture, Film


Place the cilantro, parsley, garlic and red pepper into a blender or food processor. Add the lime juice and oil. Cover and blend until the mixture is smooth.
Mix the beef and sausage in a large bowl. Season the beef mixture as desired. Shape the beef mixture into 4 (4- to 5-inch diameter) burgers.
Cook the burgers in a grill pan or 12-inch skillet over medium-high heat for 5 minutes on each side or until cooked through. Place 1 slice cheese onto each burger during the last minute of the cooking time.
Spread the mayonnaise on the cut sides of the buns. Top with the burgers, cilantro mixture and tomato slices.
For extra heat, top the burgers with fresh jalapeño pepper slices.


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Woman suffers life-threatening injuries in Birmingham house fire
by: Phil Pinarski
BIRMINGHAM, Ala. (WIAT) — A woman suffered life-threatening injuries in a house fire Monday afternoon.
The fire started in the 5100 block of Shadow Brook Trail.
According to the North Shelby County Fire Department, the woman was sent to the hospital for her injuries. Her condition has not been released at this time.
No other information has been released at this time.
Tuscaloosa officers receive COVID-19 vaccine
by Tim Reid / Jan 15, 2021
TUSCALOOSA, Ala. (WIAT) -- Dozens of Tuscaloosa first responders are beginning to receive the Pfizer COVID-19 vaccine.
The process got underway Thursday at the DCH Medical Center remote testing drive-thru facility. Deputy Chief Sebo Sanders says the process was easy to do.
Pelham FD contains brush fire, at least 1 acre of land burned
by Phil Pinarski / Jan 15, 2021
PELHAM, Ala. (WIAT) -- The Pelham Fire Department has contained an active brush fire Friday evening.
The fire has burned an acre of land as of 5:30 p.m. in the 2500 block of Pelham Parkway. PFD says 100% of the fire has been contained as of 6:40 p.m.
Prosecutors seek death penalty against Birmingham woman charged with killing 5-year-old


This column is meant for those who have a business on the ground as well as a website to market that business. You have probably got an outside firm to make your site. That makes sense, because it leaves you time to focus on your own business. But you should also remember that for the software firm that makes your site, you are just one among several clients. You must not expect it to think up a Net Marketing strategy for you. That must be done by you. And in any case, for the price that most firms charge for a site – often less than a few lakhs – they cannot afford to put a marketing expert at your disposal.
Having said that, I must add that more than half the Indian sites I visit violate elementary common sense. The website seem to be made with the intention of showcasing the web designing firm's skills rather than to sell the client's products and services. There are dozens of shortcomings and I have mentioned a few of them in my previous articles. If you already have a website, check up all these points – they will give you an idea of whether your Web design firm really has your interests in mind.
Also, ask your website designer whether he will be submitting your site to search engines. My experience is that most Indian Web designers don't. One important exception I notice is Indiamart which, in a letter to me on 7 January, said it had crossed the 500-client mark. Much before I began writting this column, Indiamart has been submitting its client' addresses to Locate India and I presume it submitst hem to all major search engines. You need a design firm like that which does not think its job has ended with making the website.
The first item to check on your website is whether your index page and other pages have meta tags.These are of the form . There is a similar tag for keywords. Unless you have a huge advertising budget or compelling content , a sizeable chunk of the visitors to your website will come from search engines, at least the first time they do so.
Another common shortcoming is the use of large pictures which take an infinit time to load. Worse, sometimes the opening page is full of large pictures without any text link So, I often end up leaving the site without going past the first page. Other culprits in the same category are Java applets, especially those used for navigation. You need to ask yourself about every image and applet on a page: "does it really add to the looks of my site? Can I do without it?" Usually, the answers to these questions are no and yes respectively.
One culprit that is cropping up on pages more often these days is Flash or Shockwave animations. These are superb technologies that allow the use of wild aniamtions without employing gargantuan files. But someone who visit your business site will probably want to download files and Shockwave is that you can't download pages that use them. If you must have these tools, restrict them to your opening screen. And doesn't have all the time in the world to see your fancy animations.
You can also do away with frames. Frames used to be considered exciting when they first emerged. And even to day, some important website use them. But they need to be used carefully. If the contents of the frame, especially the navigation frame, are too long or broad, a scroll bar crops up and destroys the aesthetics of the page. There is no dearth of such pages on the Internet. The reason is usually that the Web designer has made the page and tested it at one display resolution and one buowser and not on other. So, what looks good at one resolution or one browser looks lousy on another.
If you are using CGI or PERL files to receive forms and mail, you must make sure that they work. There is nothing more irritating for the user – or devastating for you – as to have a mail form (which is often the only way a visitor can contact you) return an answer "file not found". Hosting companies have the nasty habit of changing their rules without informing their clients. Make sure it dose not happen to you.


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What Constitutes "Supervision" of Diagnostic Tests?
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Shantae, Rise & Shine, 8-Bit Invaders, & more indie games
Frank Streva
Pictured above: Heroine Anthem Zero
After a fairly lengthy hiatus I'm back with the first round of new indie release highlights of 2017. Releases have been pretty light over the last few weeks (hence the hiatus) but I still managed to scrounge up a list of recent games that may have slipped under your radar.
First up we have Shantae: Half-Genie Hero, the fourth title in this popular series of retro-inspired Metroidvania platformers. This time around the series has dropped its traditional pixel art graphics in favor of clean and colorful HD animation. Classic characters and abilities make a return alongside plenty of new additions, including a Relic System that allows you to customize Shantae's moves to fit your play style. You can also find Half-Genie Hero on PS4, Vita, Xbox One, and Wii U.
Another noteworthy release is Rise & Shine, the latest indie game to be released under Adult Swim's publishing division. You play as a boy named Rise, who with the help of his sentient gun Shine, must save the once-peaceful world of Gamearth from an invasion by the bombastic and over budgeted forces of Nexgen. The game blends elements of traditional puzzle-platforming with fast and challenging bullet hell combat across its various game-themed, hand drawn levels. Also available on Xbox One.
8-Bit Invaders
8-Bit Invaders is the latest stand-alone installment to Petroglyph's ongoing series of RTS games. As with the previous two 8-Bit games, Invaders is heavily inspired by RTS classics like Command & Conquer and features fully destructible voxel environments. The game introduces two new sci-fi races, both of which can be used against the other factions from the previous games. In addition to the 24 mission campaign, 8-Bit Invaders also includes 10 co-op missions, multiplayer, and a new Conquer The Multiverse strategic metagame. Also available on GOG.
Reconquest
StormCube Games
Reconquest is another recent RTS that wears its C&C inspirations on its sleeve, though its reception has been far more mixed. Reconquest is set in the aftermath of WWIII, and involves two factions battling for control of Earth's remaining resources. While the game seems promising, its apparently quite rough at the moment, with a fairly limited amount of content and various issues with the UI and core mechanics. It looks like it might be worth checking out after a few more updates, though.
HoPiKo
Laser Dog
HoPiKo has been described as a really fast version of the barrel chains in Donkey Kong Country. The game is all about perfectly timing your jumps as you rocket around hundreds of levels at insane speeds. The levels are short and designed to appeal to speed runners looking beat each level in the fastest, most efficient way possible. You can also find HoPiKo on iOS, PS4, and Xbox One.
99Vidas
QUByte Interactive
99Vidas is a 16-bit love letter to classic 2D brawlers from the '80s and '90s. The game currently features 11 playable characters, each with their own unique set of upgradable combos and abilities. There are six full stages and two bonus stages so far, with the promise of more in the future. You can play 99Vidas alone or with up to three friends in both local and online multiplayer.
Alien Shooter TD
Sigma Team Inc.
The cult classic Alien Shooter series is back with a tower defense spin-off. Defend you positions against an onslaught of hundreds of alien creatures per level with your squad of elite soldiers. There are seven classes of soldiers in all, each with their own specializations and customizable loadout of weapons. In an interesting twist for the TD genre, each of your "towers" have limited ammo and must be resupplied during combat. You also have a variety of support powers to help fight the alien horde, like mines and drones.
WindThunder Studio
Finally we come to Heroine Anthem Zero, the third game in the Heroine Anthem series and the first to get an official English translation. This 2D action RPG is inspired by Norse mythology, but viewed through an anime lens. Character progression is tied more closely to your weapons and gear than your stats, and your weapons also have uses outside of combat when it comes to solving the game's various environmental puzzles. Be aware that this is only the first chapter of the game, with the rest being released episodically in the future.
That's all for this week. I'll be back soon with more indie release highlights.
8-Bit Invaders Alien Shooter TD Heroine Anthem Zero Rise & Shine Shantae: Half-Genie Hero
Ley Lines, Space Hulk: Deathwing, Hunger Dungeon & more overlooked indie games
Sanctus Reach, Hive Jump, Death's Hangover & more new indie games