text:
Investigating a neuronal subcellular transcriptome by the novel technique of RNA TU-tagging, in a normal and ALS-related mouse model.
Lead Research Organisation: University College London
Department Name: Institute of Neurology
Amyotrophic Lateral Sclerosis (ALS, 'motor neuron disease') is a devastating neurodegenerative disorder which causes progressive loss of muscle function and paralysis. ALS leads to death, usually caused by the inability to breathe, on average only 3 years after diagnosis, with a lifetime risk of ~1 in 250 by 85 years old.
The principal cells affected in this disease are nerve cells called motor neurons (MNs). MNs connect the brain to the muscles therefore making movement possible. MNs progressively die during the course of ALS. MNs are amongst the largest cells of the body. Their main body lies in the spinal cord and contains numerous thin branching processes called dendrites. They also have one thin process, named the axon, which extends from the spinal cord out to each of our muscles. A single axon can measure over a meter, running from the spinal cord to ends of our fingers or toes. The connection between the MN and muscles is called the neuromuscular junction (NMJ).
All cells in an individual's body, although very diverse from each other, contain the same DNA, the genetic material that gives instructions to each cell. So the identity of each cell type (whether the cell is a nerve cell or a heart cell, for example) is the result of which regions of DNA are active and produce another type of chemical called RNA. RNA carries all the necessary information for the cell to function. The sum of all the RNA in a cell, named the transcriptome, is the signature that characterizes each cell type.
Knowing one cell transcriptome provides insights into its biology and helps determine the causes of disease. This is particularly relevant with MNs in ALS since there is good evidence showing that the biological processes linked to RNA 'metabolism' are primarily affected in ALS.
Importantly, we now know that RNA is transported and functions in different regions within an individual cell; in MNs it is transported in axons and to NMJs for specific roles. NMJs and axons are thought to be the first parts of the MNs to be affected in ALS.
Therefore it is important to know which RNAs are present in cell bodies and dendrites, and in axons, and at neuromuscular junctions of MNs, to understand how they function normally and what goes wrong in ALS.
It is possible to isolate and identify RNA from neurons and their axons when these are artificially grown in a culture dish or when cell bodies are dissected out under a microscope from fixed tissues. These findings have shown that thousands of different RNA species are actively transported to the axons, but it is difficult from these experiments to extract information that is relevant to mature neurons in their natural context in vivo and therefore to disease.
We will work with a new technique, 'TU-tagging', which has already been successfully used in mouse and which allows us to 'tag' RNA in specific cells in the context of a living animal. The tagged RNA can then be isolated and identified through high-throughput sequencing. We will apply this technique to MNs so for the first time we can isolate and identify RNA from MNs cell bodies and dendrites, and from their axons and NMJs in the living adult mouse.
We will work with normal mice, and with a new mouse model that we have developed that has a defect in a gene, Tardbp (also known as Tdp-43) that causes ALS. From our current work we know defects in this gene give an aberrant RNA profile in the MN cell bodies of this mouse. Currently no Tdp-43 mutant mice exactly model human ALS, but they teach us a great amount about how Tdp-43 functions in the normal and abnormal state.
These results will be extremely helpful in furthering our understanding of MN biology and of what causes these cells to be so specifically vulnerable in ALS.
This project will help research in the many other diseases in which RNA metabolism in different cell regions is important, and in response to nerve injury where again it plays a key role.
Coding and non-coding RNAs are transported to different subcellular localisations for local translation and regulation. Localised 'RNA metabolism' is important in polarised cells such as neurons, but techniques to study RNA localisation are not able to isolate subcellular transcriptomes in vivo.
Motor neurons (MNs) have cell bodies in the spinal cord, numerous arborizing dendrites and they connect to muscle fibres at the neuromuscular junction (NMJ) through an axon that can exceed 1 meter in length.
We are interested in MNs because of our research into Amyotrophic Lateral Sclerosis (ALS), a fatal neurodegenerative disorder that is characterized by the progressive loss of motor neurons that 'die back' from NMJs. ALS-causative mutations in RNA-binding and -transport genes (TARDBP 'TDP-43', FUS), and in C9ORF72 that induce RNA foci - have highlighted aberrant subcellular RNA metabolism in ALS pathogenesis.
Thus there is a clear need to identify the neuronal subcellular transcriptome in vivo to understand normal function, and dysfunction in our paradigm disease, ALS.
We will use a new technique, 'TU-tagging' (already successful in mouse) to identify the subcellular transcriptome of three compartments of adult motor neurons in normal mouse and in a mouse with a mutation in Tdp-43. Our preliminary data from a Tdp-43 mutant we are characterising shows disruption of many downstream genes including b-synuclein and tau.
TU-tagging works through the cell-specific expression of an enzyme (UPRT) and treatment with a drug (4TU), allowing incorporation of thio-uridine into RNA in specific cell types. Thio-uridine RNA is biotinylated and then pulled-down. We will use TU-tagging to identify RNA from (1) MN cell bodies and dendrites, (2) MN axons, (3) NMJs.
This will allow us insight in the biology of MNs, axons and NMJs and their susceptibility in disease.
TU-tagging is applicable to all models of normal function and disease, and of neuronal injury.
Planned Impact
The impact of this research lies in two areas (1) understanding the biology of the transcriptome in subcellular locations, (2) amyotrophic lateral sclerosis and the TDP-43 proteinopathies. These two impacts are quite different in that (1) provides a new technique (albeit previously used in mouse) that we believe will become a standard approach to isolating the subcellular transcriptome and so with very broad impact, whereas (2) tackles a biomedical issue, well-known neurodegenerative diseases for which no cure exists.
(1) TU-tagging is a technique that is relevant to every study with a need to catalogue and quantify the localised RNA transcriptome within a cell type. This has obvious application to early development of the Drosophila and mammalian embryos, but also to ALL other studies in which subcellular RNA pools are important.
Therefore we envisage this becoming a standardly used technique, only limited currently by the availability of suitable mouse models. We note that with the number of freely available mice with individual gene mutations and Cre transgenics with precisely defined patterns of expression set to rise dramatically over the next few years, TU-tagging will be very widely applicable to both academic and industrial applications. Knowing the subcellular transcriptome will have impact on academic studies of all areas of biology and also on industry studies for example in highlighting new mechanisms and targets, notably in non-coding RNAs. One immediate application for both academic and commercial interests is in the neuronal response to injury which involves local translation to form a signalling complex that is carried to the cell body.
(2) ALS is a devastating incurable mid-life disorder for which no cure or effective treatment exists. It is clear that localised RNA biology is involved within neurons and so our research will shed light on pathways and networks that are important for motor neuron health and disease. This will have impact on the ALS community.
As our paradigm, we have chosen to work with a Tdp-43 model related to ALS - this gene/protein is also dysfunctional in many common neurodegenerative disorders, including Alzheimer disease and Parkinson disease, and so this project may well also have a wider impact as more commonalities are found in the 'TDP-43 proteinopathy' neurodegenerative diseases, particularly those clearly involving disruption to RNA metabolism disruption.
Thus our findings may benefit biotech/pharma by providing new insights and new targets for the development of therapeutics for ALS, and possibly other RNA disorders. It is intriguing also that several of the new ALS genes also play roles in specific cancers when translocated and so it is possible this research may have an impact on cancer studies also.
We note also that the sub-cellularly localised RNA transcriptome also plays a role in the response to nerve injury and thus our approach should be of interest in studying the impact of neuronal damage.
Finally, increasingly sophisticated techniques are being used for making RNA a therapeutic molecule, and it is likely that in the future knowledge of the localised transcriptome may further refine these efforts.
Mar 14 - Sep 16
MR/K018523/1
Elizabeth Fisher
2.1 Biological and endogenous factors (100%)
University College London, United Kingdom (Collaboration, Lead Research Organisation)
University of Padova (Collaboration)
MRC Harwell, United Kingdom (Collaboration)
Elizabeth Fisher (Principal Investigator)
Vincent Plagnol (Co-Investigator)
Abdelkarim S (2016) CHCHD10 Pro34Ser is not a highly penetrant pathogenic variant for amyotrophic lateral sclerosis and frontotemporal dementia. in Brain : a journal of neurology
Bunton-Stasyshyn RK (2015) SOD1 Function and Its Implications for Amyotrophic Lateral Sclerosis Pathology: New and Renascent Themes. in The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry
Cortese A (2014) Widespread RNA metabolism impairment in sporadic inclusion body myositis TDP43-proteinopathy. in Neurobiology of aging
De Giorgio F (2019) Transgenic and physiological mouse models give insights into different aspects of amyotrophic lateral sclerosis. in Disease models & mechanisms
Devoy A (2017) Humanized mutant FUS drives progressive motor neuron degeneration without aggregation in 'FUSDelta14' knockin mice. in Brain : a journal of neurology
Fratta P (2014) Correlation of clinical and molecular features in spinal bulbar muscular atrophy. in Neurology
Fratta P (2018) Mice with endogenous TDP-43 mutations exhibit gain of splicing function and characteristics of amyotrophic lateral sclerosis. in The EMBO journal
Fratta P (2014) Sequencing analysis of the spinal bulbar muscular atrophy CAG expansion reveals absence of repeat interruptions. in Neurobiology of aging
Fratta P (2014) Profilin1 E117G is a moderate risk factor for amyotrophic lateral sclerosis. in Journal of neurology, neurosurgery, and psychiatry
Fratta P (2015) Screening a UK amyotrophic lateral sclerosis cohort provides evidence of multiple origins of the C9orf72 expansion. in Neurobiology of aging
Research Databases and Models
Description MRC ResearchGrant (Uprt) MR/K018523/1
Description Rosetrees award
Organisation Rosetrees Trust
Description Understanding disease mechanism: Sub-cellular translation in motor neurons in health and in amyotrophic lateral sclerosis
Funding ID M438-F1
Title A new mouse model of motor neuron degeneration (FUS ALS)
Description A new genetically engineered mouse model of FUS ALS
Type Of Material Model of mechanisms or symptoms - mammalian in vivo
Impact Several labs now working with this model
Title Mouse model Sod1 D83G
Description Mouse with endogenous mutation in ALS gene (Sod1 D83G)
Impact Paper submitted currently, after which the mouse will be made freely available. Second paper being written. We anticipate this mouse will be of interest to the ALS research community.
Title UPRT development
Description With Pietro Fratta, developing the UPRT transcriptomic analysis system in our mouse models.
Type Of Material Technology assay or reagent
Impact pending
Title mouse embryonic stem cells for Down syndrome
Description Manipulated mouse embryonic stem cells to model aspects of Down syndrome
Type Of Material Cell line
Impact academic papers
Title FUS homozygotes MEFs
Description Working with a mouse model, an in vivo model, to produce IMMORTILISED cell lines so that we can drop our animal useage.
Type Of Material Database/Collection of data
Impact Reduced mouse numbers
Description Analysis of the FUS mouse translatome, Fratta, UCL
Department Marie Curie Palliative Care Research Department
PI Contribution contribution of the unique FUS Delta14 mouse model
Collaborator Contribution RiboTagging and ChatCre breeding to pull down polysomes from the Delta14 mouse
Impact Multidisiplinary output. No outcomes yet as just started.
Description MMON
Organisation MRC Harwell
PI Contribution Collaboration with the Mouse Models of Neurodegeneration lab at MRC Harwell, analysis of homozygous and heterozygous mice
Collaborator Contribution Breeding, inbreeding onto another background, and phenotypic analysis of homozygous and heterozygous mice.
Impact Inbred mice on different backgrounds. Cohorts of mice of different ages, sex-matched with littermate controls, wildtype, heterozygous, homozygous, for phenotypic analysis. Analysis of different phenotypes ranging from behavioural through to physiological.
Description UPRT and localised transcript study
Department Institute of Neurology
PI Contribution Co-grant awardee, mice and space.
Collaborator Contribution direct supervision of joint staff
Description studying ribosomal proteins
Organisation University of Padova
Department Department of Neurosciences
PI Contribution Access to a unique mouse model of FUS ALS (Delta14)
Collaborator Contribution Analysis of ribosomal proteins
Impact No outputs yet
Description Set up new ALS Seminar Series
Results and Impact Set up an academic seminar series on Amyotrophic Lateral Sclerosis and similar topics, but as this is open to the lay public we have had a surprising interest and attendance from sixth form pupils and undergraduates.
Description Visit by fundraisers from MNDA
Results and Impact Fundraisers from MNDA visited to learn more of current research. These people are well-informed on issues regarding MND and care for people with MND, but are not scientists and do not necessarily have easy access to researchers.
Better informed fundraisers.


text:
Blue Star Mom Remembers Her Son
July 2006, Feature
In September, Jan Martin appeared on the cover of the premiere issue of Edmond Outlook. She and the other women pictured are Blue Star Mothers, a group that sends care packages to military servicemen. At the time, Martin's son, David, was about to be deployed to Iraq.
Private First Class David Martin was a member of the 2nd Battalion, 502 Infantry Unit of the 101st Airborne Division, stationed in Baghdad. One week after his arrival, his company took command of the city and David volunteered for the first patrol.
"They were told it would be dangerous," Jan said. "They knew it was an insurgent stronghold and there would be IEDs (improvised explosive devices)."
From intelligence reports, they expected to find five IEDs in the area they were patrolling. David was on the top of the Humvee, looking for the homemade bombs, having found the first four.
As their vehicle rolled over it, the fifth IED, buried deep beneath the road bed, exploded. On October 31, 2005, Pfc. David Martin and the other three soldiers in the vehicle were killed, including his First Sergeant, a 17-year veteran of special operations.
To date, more than 2,500 American soldiers have been killed in the war in Iraq. More have lost their lives in Afghanistan. Of those, 47 listed Oklahoma as their home state on military records. At least six were from the Oklahoma City area.
David Martin grew up in Edmond and graduated from Edmond North High School in 2002. Before joining the Army, he was in the ROTC program at UCO for two years. He turned down two scholarships to join the war effort.
David worked at Snyder's IGA and often went the extra mile to help those in need. "There was an elderly couple who would call Snyder's and David would deliver their groceries," Jan said. After David's death, the couple sent the Martins a condolence card letting them know how kind David had been and how he would call to check on them.
"He was very caring," Jan said. People from all over the community turned out for David's funeral, to honor their hometown hero.
"The Edmond Police and Fire Department gave us a lot of support and it was great. We really needed it," Jan said. "The community was just beautiful. The streets were lined and people were waving flags in a show of support for this fallen soldier."
"Edmond is a community of families and they could relate to our loss," Jan said. "They let us know they felt our grief."
The Martins love their community and their country. David's oldest brother, Neil is an Edmond Police officer and older brother Andrew is a communications officer for the city of Edmond. His sister Allison works for the Edmond YMCA and younger brother Daniel is with the parks department at Lake Arcadia.
"We're a tight-knit family," Jan said. The Martins enjoyed camping at the lake together and David was a real outdoorsman, hiking and kayaking with his brothers.
Jan keeps David's medals, patches, challenge coins and special photos in a wooden box from the 101st with his name on it. During his year and one week in uniform, David was awarded the Bronze Star, the Purple Heart, the Combat Infantry Badge and the Army Achievement Medal. In nearly all of his pictures, David is smiling and giving the thumbs up sign, a reflection of his positive personality.
"He always had a smile on his face and always had something funny to say," Jan said.
While his unit was training at Fort Campbell, Kentucky, David organized a kite-flying club. "David wasn't one to drink and party, but he wanted something to do," Jan said. On his last visit home in September, the Martins flew kites together in Mitch Park.
"It's a good memory for us," Jan said.
David's dog, Strike, died just before he came home on leave and he told his family, "I hope that's not an omen about me going to Baghdad." Jan says now when she is missing David, she thinks about him and Strike walking along a lake together in heaven.
Jan got involved with Blue Star Mothers when David was finishing up basic training because she wanted to do something proactive to support the troops. Now, it helps her cope with the loss of her son.
"Doing something positive for them helps me," she said. Jan knows that every soldier who receives one of the care boxes has a desire to protect and serve our country.
"They know they will be in grave danger, but they still sign up for it," she said. "David believed he was stepping up to the plate; that it was up to the young people to take a stand and stop the terrorists . . . . He thought they should be stopped, even if it meant the ultimate sacrifice."
In the very beginning, after David's death, Jan said she didn't feel comforted by God and "was stunned silly." She said during the really bad times she would try to deal with it, but the pain was enormous. Nearly always, someone would cross her path and offer support. After a while she recognized the divine plan.
"God knows I'm a people-oriented person, so He'd send me these people and they'd share their wisdom," Jan said. "When I would least expect it, there would be someone with a comforting message."
Jan keeps in touch with the families of those who died with David. "We're all trying to cope and keep living," she said. "We all have a sense that they are all together in heaven."
She also communicates by email and care packages with David's friends in Iraq, many of whom are now on their second tour of duty.
"We can't forget our troops and we can't thank them enough," Jan said. "The war is being fought over there so that it doesn't happen here."
Jan also finds solace in her favorite picture of David. It was taken on a hiking trip in Colorado with David on the summit of a mountain. He said it was how he wanted to be remembered if anything happened.
"He told me, 'I made it to the top of the mountain and it's blue skies all the way,'" Jan said. He's giving a thumbs up in the photo, meaning it's okay. He's made it to the top of life's mountain and now he's in heaven with God. "I keep it on my desk and I look at it every day and say, 'I'm trying.'"
There are currently 136,000 American soldiers serving in Iraq and another 17,000 in Afghanistan. Over the past two years, Blue Star Mothers of Edmond and North Oklahoma City have sent out more than 4,000 care boxes and they are always collecting donations for the next mailing. Items needed include presweetened drink mix, sunscreen, snacks in crush-proof containers and personal care items like toothbrushes, toothpaste, razors and shampoo. Donations can be left at the Edmond Armory or the Blue Star Mothers' office at 2801 E. Memorial.
"It may seem to people, 'Oh, they don't need that,'" Jan said. "But if it brightens up their day for a little bit – mission accomplished. We can make a powerful difference in our soldiers' lives."

redpajama_set_name: RedPajamaCommonCrawl


text:
import VueNotify from './Notify.vue'

export default {
  install (Vue, options = {}) {
    // Create component instance
    let Constr = Vue.extend(VueNotify)
    let Notify = new Constr()

    // Apply configuration
    Notify.options = Object.assign(Notify.options, options)

    // Mount it
    let vm = Notify.$mount()

    // Add it to the Vue application
    document.querySelector('body').appendChild(vm.$el)

    // Create generic method
    Vue.$notify = Vue.prototype.$notify = (msg, type = 'info', options = {}) => {
      Notify.addItem(type, msg, options)
    }
    Vue.$notify.info = Vue.prototype.$notify.info = (msg, options = {}) => {
      Notify.addItem('info', msg, options)
    }
    Vue.$notify.success = Vue.prototype.$notify.success = (msg, options = {}) => {
      Notify.addItem('success', msg, options)
    }
    Vue.$notify.error =
      Vue.prototype.$notify.error =
        Vue.$notify.danger =
          Vue.prototype.$notify.danger = (msg, options = {}) => {
            Notify.addItem('error', msg, options)
          }
    Vue.$notify.warning = Vue.prototype.$notify.warning = (msg, options = {}) => {
      Notify.addItem('warning', msg, options)
    }
    // Create setTypes method
    Vue.$notify.setTypes = Vue.prototype.$notify.setTypes = (types) => {
      Notify.setTypes(types)
    }
    Vue.$notify.removeAll = Vue.prototype.$notify.removeAll = () => {
      Notify.removeAll()
    }
  }
}

redpajama_set_name: RedPajamaGithub


text:
es una serie emitida en el 2003 y  producida por AIC. La temática es sobre ordenadores y humor ecchi.

La serie original cubre 5 historias, de 3 capítulos de 12 minutos cada una.

Argumento
La historia de BPS gira en torno a un talentoso y misterioso programador llamado Akira Shirase. Por sus increíbles habilidades, es contactado por varias personas (curiosamente, todas ellas muy parecidas y de nombre Akizuki Kaoru) para hacer todo tipo de trabajos extraños relacionados con ordenadores y seguridad. Al principio, los capítulos giran en torno a trabajos que exhiben sus habilidades, pero más adelante en la serie, reaparecen personajes para ofrecer ayuda o dar giros al guion. Se han dado pistas del colegio al que Shirase fue, y finalmente aparece en el último episodio, cuando algunos personajes muestran sus relaciones con los demás. A pesar de todo, la serie no tuvo continuación.

Personajes

 Es el personaje principal de la historia. Akira es un talentoso programador conocido en el mundo hacker como Battle Programmer Shirase (normalmente abreviado BPS). Es un genio de la informática y la seguridad, y puede hacer cualquier cosa en cualquier PC o móvil.

 Misao es la sobrina-nieta de Akira. Es una chica tímida a la que le encanta pasar tiempo con Akira, a quien llama . Se preocupa mucho por él y es normal verla cocinándole o preocupándose por él. A veces, Misao dice , que no significa nada (puede ser un guiño para los fanes de las chicas de animes). Misao parece muy joven para ser una estudiante de 6º grado.

 Yoriko es una chica que trabaja para la marina de los Estados Unidos. Su rango es Comandante Yoriko Yonoki. Conoce a Akira cuando él es visto de regreso a casa tras esperar enfrente de una tienda de libros. Ella había estado siguiendo a Misao y pregunta si podría ir a su casa. Parece tener los mismos conocimientos de informática que Akira y en un episodio le pide que se una a ella en la Guardia Nacional como su compañero. También le pide que vaya a América con ella, y parece tener fuertes sentimientos hacia él.

 Motoki Sae es la profesora de Misao. En un capítulo intentó juntar a Misao con un chico de otra clase. Más adelante se la ve bebiendo en un callejón donde se encuentra con Akira, que no ha visto desde el instituto. Ella estaba siendo forzada a tener una entrevista matrimonial, y como no quería ir se emborrachó en el callejón.

Música
En la serie aparecen dos temas. El del opening se llama "Suddenly", por Naomi Amagata. El ending es "Pure Enough", por Yuki Matsuura.

OST

   01 OP Suddenly vo.天方直美
   02 M-1 Jiken no Yokan 1
   03 M-2 Jiken no Yokan 2
   04 M-3 Jiken no Yokan 3
   05 M-4 Shigoto no Irai kara Juchuu made no Kinpaku
   06 M-5 Shuugetsu no Kattou 1
   07 M-6 Shuugetsu no Kattou 2
   08 M-7 Jiken Hassei 1
   09 M-8 Jiken Hassei 2
      Denshi Tatakai 1
   10 M-10 Denshi Tatakai 2
   11 M-11 Denshi Tatakai 3
   12 M-10 Denshi Tatakai Hissatsuwaza
   13 M-13 Narration Back
      Hatsuban Ohanashi no Shime no Ongaku
   14 M-15 Shirase Norokusa Ecchi
   15 M-16 Shirase Kakko ii kamo Shirenai  Shirase
   16 M-17 Shirase Kakko yoku Saru
   17 M-18 Shirase
   18 M-19 Sabishii Misao
   19 M-20 Tanoshige na Misao
   20 M-21 Setsunai Misao
   21 M-22 Tantan to Borero
   22 M-23 Gyagu 1
   23 M-24 Gyagu 2
   24 M-25 Kiki
   25 M-26 Nostalgic na Kaisou Shuun
   26 M-27 Setsunasa
   27 M-28 Kouchou no Toujou
   28 M-29 Oosaka no Kenka
   29 M-30 Eroi Shiin
   30 M-31 Kegon ni Sougon na Hade ni Yuusou na Kaiketsu
   31 M-32 Kehaisae Kaiketsu
   32 M-33 Tanoshii Kaisuiyoku
   33 M-34 Yoriko no Teema
   34 ED Pure enought vo.松浦有希

   Por Seikou Nagaoka.

Agradecimiento al público
El último capítulo hizo el extraño gesto de pedir perdón por terminar la serie, no solo para los telespectadores japoneses, sino para los de la comunidad fansub. Se dirigieron a "los que disfrutaron el show en TV, y aquellos fuera del área de emisión que tomaron medidas especiales para ver el show en sus pantallas, y todos los que lo vieron subtitulado sin permiso en el extranjero."

Enlaces externos

Anime de 2003

redpajama_set_name: RedPajamaWikipedia


Line a 9-inch x 9-inch brownie pan with parchment paper.
Add the butter, cocoa powder and marshmallows to a microwave safe bowl and heat until the marshmallows start to melt.
Stir once, and then return to the microwave until the marshmallows are completely melted.
Stir until the mixture is uniform in appearance.
Add the cornflakes and chocolate chips and stir to coat evenly.
Empty the mixture into your prepared pan and use another sheet of parchment paper to flatten the top and press the mixture into the corners of the pan.
Refrigerate until set, and slice into bars.


Tucked away in the southern countryside of Barbados, on 8 acres of a former sugar plantation, is the magnificent Foursquare Rum Distillery and Heritage Park. Foursquare is the name of the oldest sugar plantation (1636). This working rum factory is well known for its collection of rums, producing a wide range. Foursquare Rum Distillery is the proud winner of the Rum Distillery of the Year in November 2016 at the IWSC.
Foursquare Spiced Rum is a combination or rare island spice and specially aged rum blended in accordance with a centuries old secret formula known only to generations of the Seale family. There is an immediate fragrance of star anise, vanilla, cloves and cinnamon, with some orange following along. On the palate fresh anise comes through prominently, developing into a complex array of sweet Caribbean spices with great balance between the spices and herbs; finishes with a slight ginger kick. Perfect for a spiced Mojito we think!


text:
Gwen Stefani Busts Out No Doubt and Queen Classics for "Carpool Karaoke"
James Corden, like the rest of the world, has been a bit preoccupied with Beyoncé's Lemonade of late, but following his own interpretation of the visual album, the late-night television host has returned to his sensationally viral segment "Carpool Karaoke."
This time it's Gwen Stefani in the passenger seat, and the No Doubt frontwoman turned solo artist turned The Voice coach sang along to some of her biggest hits. After an emotionally charged "Don't Speak," Corden and Stefani relive the mind-boggling lyrics to "The Sweet Escape," belt some of her solo hits, chat about emojis and then pick up some of Hollywood's biggest stars.
Joined by George Clooney and Julia Roberts, the group hit the HOV lane for "Hollaback Girl" before a dramatic delivery of Queen's "We Are the Champions."
Watch things get B-A-N-A-N-A-S in the player below.
Stefani's latest LP This Is What the Truth Feels Like is out now.
More Gwen Stefani
Here's the Full List of Grammy Performers
The 62nd annual Grammy Awards will take place on Sunday evening (January 26), and the list of performers has been finalized. The star-st...
Korn, Nickelback, Imagine Dragons Read Mean Tweets on 'Kimmel'
Jimmy Kimmel has been turning the table on trolls with his "Mean Tweets" segment on Jimmy Kimmel Live! for years now, and he brought back th...
Watch Mariah Carey, Adele, Elton John, Red Hot Chili Peppers on Christmas Edition of 'Carpool Karaoke'
The Late Late Show host James Corden has offered up another instalment of his hit Carpool Karaoke segment — and this one's a huge holiday su...
Justin Timberlake, Zooey Deschanel, Gwen Stefani Offer Up New Songs for 'Trolls' as Soundtrack Detail Emerge
After first teasing the upcoming Trolls movie with Justin Timberlake's "Can't Stop the Feeling," the rest of the hair-raising flick's sound...
Gwen Stefani "Make Me Like You"
As previously reported, No Doubt frontwoman Gwen Stefani is going the solo route again this year, with her This Is What the Truth Feels Like...


Transcripts of the August 28, 2013 HHS Public Meeting on Matters Related to Protection of Human Subjects and Research Considering Standard of Care Interventions have been posted. The transcripts were created by OHRP staff from the video captions and can be accessed from the Spotlight section of OHRP's home page http://www.hhs.gov/ohrp/. Videos of that meeting can also be accessed from that site.


text:
After getting his law degree Gary Culliss started an Internet company, Direct Hit Technologies.
Tiffany Mickel has just started her law degree, but she has already founded a company, Heiritage, that features famous African-American women, such as Angela Davis, Harriet Tubman, and Shirley Chisholm, on playing cards. Ms. Mickel is a joint J.D.-M.B.A. student at the University of Virginia.
University of Denver law grad John H. Works was CEO of Rompetrol, an international oil and gas conglomerate.
Karen E. Laine, a graduate of the McKinney School of Law (IUPUI) founded Two Chicks and a Hammer with her daughter Mina Starsiak Hawk, and is one of the stars of HGTV's show Good Bones.
Cheryl Numark tired of law practice, opened an art gallery in the D.C. area and is now an art advisor. Steve Davis was a human rights lawyer before taking over direction of Corbis, an archive of photographs owned by Microsoft. Donald Watkins, a University of Alabama law graduate, attempted to revitalize the fortunes of the Minnesota Twins by buying the team. He also attempted to purchase several other professional sports teams. Truman Gibson practiced law in Chicago before becoming a member of Harry Truman's "black Cabinet"; he also became a successful boxing promoter.
Nina and Tim Zagat practiced law for decades before deciding to make their hobby of dining out into a full time career. They now publish the enormously popular Zagat Restaurant Survey. Attorney Daniel Dolan decided matchmaking was more fun than corporate deal-making, and is the President and CEO of It's Just Lunch, a dating service for professionals. Bernard Jarrier-Cabernet was a lawyer for five years before becoming a painter, and then a restauranteur and then a publisher. Samantha Daniels is a professional matchmaker. She co-produced the comedy Miss Match, which starred Alicia Silverstone.
Texas-born Marjorie Scardino earned a law degree from the University of San Francisco and became the first woman to head a company listed on the London Stock Exchange.
Colonel Sanders studied law before launching the Kentucky Fried Chicken restaurants.
Frederick Furth practices law and makes wine in Sonoma County, California. Jess Stonestreet Jackson (founder of the Kendall-Jackson Winery) is also a lawyer. Anthony Avis put his legal training to use in management at Hammonds United Breweries. His book The Brewing Industry 1950-1990 recounts his adventures.
California lawyer Steve Pitcher turned to writing about wine after two decades of writing about law. Lawyer Robert Parker publishes the Wine Advocate. Other lawyers who combine wine with advocacy include Paul Beveridge (yes, that's really his name), Jim Paras, and Robert M. Cohen. LSU Law graduate William McGehee and his partner Charles Caldwell started Tin Roof Brewing Company in Baton Rouge. Rick Rosenfield and Larry Flax quit law for pizza and founded California Pizza Kitchen in 1985; it's now a leading restaurant chain.
Former New York lawyer Julie Menin owned a restaurant that was destroyed in the September 11th terrorist attacks. Randy Paragary is a McGeorge Law School graduate who runs numerous Sacramento eateries.
Georgetown Law School graduate Marshawn Evans appeared on Donald Trump's The Apprentice, founded 3M Edge Sports and Entertainment, and wrote Skirts in the Boardroom: A Woman's Survival Guide to Success in Business & Life (Wiley 2008).
Adam Epstein has launched a business that lets people become sports announcers.
Philadelphia's Alycia Kinchloe has launched the Blazer Beauty Bar . Meanwhile, she continues to run her law firm, Kinchloe Law (@KinchloeLaw) and host her podcast, The Growth Goal.
Jeff Furman, co-founder of the successful Ben & Jerry's ice cream company, earned a J.D. from NYU in 1968, and practiced law for a while and then doing some other things before eventually going into business with Ben Cohen.
Justin Wolverton, a corporate lawyer in Los Angeles, founded Halo Top Ice Cream in 2012.
Before he went to law school, Michael Sipos worked as a bartender in the French Quarter. He explains how that profession helped him learn some skills he uses as an attorney.


text:
The Individual freestyle test grade IV equestrian event at the 2004 Summer Paralympics was competed on 24 September. It was won by Ann Cathrin Lubbe, representing .

Final round
24 Sept. 2004, 14:00

References

2004 Summer Paralympics events


text:
Attorney General's Marijuana Merger Investigations Could Get Him Impeached Under New Resolution
Three dozen members of the House of Representatives introduced a resolution on Tuesday calling for an inquiry into the possible impeachment of Attorney General William Barr because he "abused the power of his office" to improperly investigate marijuana businesses and allegedly engaged in other unlawful conduct.
The resolution, which is being led by Rep. Steve Cohen (D-TN), states that the attorney general "has taken deliberate actions that violate the rights of the American people, assault the principle of impartial administration of justice, and undermine the constitutional structure of separation of powers across three co-equal branches of Government."
It says that Barr "abused the power of his office to initiate pretextual antitrust investigations into 'unpopular' American corporations in the cannabis, automobile, and technology industries."
That allegation comes from a Justice Department whistleblower who publicly revealed this month that 10 antitrust investigations targeting cannabis companies were launched at the direction of the attorney general, who was said to have made the decision based on his personal animus for the industry.
"He's using antitrust not for the purpose of helping consumers and businesses that might be disadvantaged by monopoly, but doing it as a vehicle for his social policies, which is unheard of," Cohen told Marijuana Moment in a phone interview, referring to the attorney general.
The whistleblower didn't see "any practices that are antitrust in nature, but they said the problem was on the fifth floor, which is where Barr's office resides and apparently he doesn't like marijuana so he went after them," the lawmaker said.
At a House Judiciary Committee hearing last week, the whistleblower argued that there wasn't an appropriate basis for the investigations, which at one point accounted for one-third of Justice Department Antitrust Division cases.
The department's Office of Professional Responsibility, to which the complaint was referred, ultimately concluded that no rules had been violated in ordering the investigations, regardless of the allegation about Barr's prejudice. That said, the matter is also separately being looked into by the inspector general, according to a June memo.
"I think it's just unfortunate what they're doing," Cohen said of the memo.
While the new resolution states that other unrelated "investigations misused the resources Congress appropriated for the Department of Justice to harass and intimidate companies based on President Donald J. Trump's whims and without a proper legal predicate for such investigations," the whistleblower specified that the marijuana-specific inquiries were connected to Barr's alleged bias, not the president's.
However, he did make clear that department resources were wasted and questioned the factual basis for the investigations. All told, across six of the 10 investigations for which data are available, 5,965,000 documents were produced by the cannabis companies under inquiry. A small fraction were actually reviewed by Justice Department personnel.
To Read The Rest Of This Article By Kyle Jaeger on Marijuana Moment
The post Attorney General's Marijuana Merger Investigations Could Get Him Impeached Under New Resolution appeared first on L.A. Cannabis News.
Youth becoming addict of Cannabis and its effect on human
High Times Enters into Agreement to Acquire California Cannabis Delivery Service Mountain High


Attention : Information Notice regarding Admission forms for Lower KG at The Bishop's School, Camp and for Nursery at The Bishop's Co-Ed School, KLN & Undri for Academic Year 2019-2020 will be available online in the month of December 2018.
Please write your suggestion for the selected vote option.
Welcome To The Bishop's Education Society Upgraded Website.
Admission Notice for 2018-19 has been released, Please check Admission notice section.
Please note : Bishop's Online is available only to the students & parents of The Bishop's School.
Click on the section Bishop's Online. Enter username and password.
The student can login with username : "190023" and password :"190023"
The Parent can login with username : "190023P" and password : "190023"
Please Change Your Password After You Login To Ensure Security For Your Account.
Help file to configure email account on iPhone & Android.


A pure silver charm with your pet's pawprint in pure silver. This is suitable for attaching to charm bracelets or necklaces.
The charm will be shaped according to the pawprint, just like our cut-out hand and footprint pieces or be a small rectangle as shown in the image. Due to its small size, only an initial can be engraved on the back. You can also choose the little word tag which can have a full name or date on it.


How Do Americans Poll On Russia?
President Donald Trump on Friday had his first face-to-face meeting as U.S. President with President Vladimir Putin of Russia. Confusion ensued as Russian officials claimed that Mr. Trump accepted Mr. Putin's denials that Russia engaged in cyberhacking the 2016 U.S. elections. The U.S. denies Russia's version of the meeting. Many Americans, both Democratic and Republican, share a strong mistrust of Russia.
We look at where American opinion is trending regarding the U.S.-Russia relationship with Dina Smeltz, senior fellow of Public Opinion and Foreign Policy at the Chicago Council on Global Affairs.


text:
Hatchery Supported Trout Waters Open April 2
Author: Mindy Wharton/Tuesday, March 15, 2022/Categories: Conserving, Fishing, From the Field, Enjoying, Home, News
RALEIGH, N.C. (March 15, 2022) – The N.C. Wildlife Resources Commission will open approximately 1,000 miles of Hatchery Supported Trout Waters at 7 a.m. on April 2. The season will run through Feb. 28, 2023.
The Wildlife Commission has posted the full Hatchery Supported Trout Waters stocking schedule on its website to give trout anglers opportunities to plan fishing trips in advance. The schedule is searchable by county and month and provides information on what days each water is being stocked.
Hatchery Supported Trout Waters, which are marked by green-and-white signs, have been closed since Feb. 28 for stocking. Most trout are stocked April through June, with a few streams selectively stocked in July. Overall, staff will stock nearly 964,000 trout — 96 percent of which will average 10 inches in length, with the other 4 percent exceeding 14 inches in length.
Hatchery Supported Trout Waters are open to public fishing; however, many of those miles are privately owned so officials with the Wildlife Commission urge anglers to respect the property they are fishing on and remember that landowners can take away access if they feel their property is being misused.
Anglers can help prevent the loss of public access to fishing by:
Respecting private property and landowners at all times.
Removing all trash and litter from fishing and parking areas.
Parking only in designated areas and leaving driveways open for traffic.
Closing and/or locking gates after use.
Reporting wildlife violations by calling 1-800-662-7137.
For a complete list of all Hatchery Supported Trout Waters, as well as trout maps, the complete stocking schedule, and daily stocking updates on Hatchery Supported Trout Waters, visit the Wildlife Commission's trout fishing page.


The health and safety of residents is important to the community. Due to concerns for health, safety, sanitation, noise, and humane treatment, the only pets permitted in Residential Life facilities are small fish and aquatic turtles (subject to the exceptions below).
Pets must be retained in fresh water aquariums that may not exceed 10 gallons in size. Pets and their environment must be properly cared for and maintained. Failure to follow the pet policy may result in your pet privileges being revoked and/or judicial action being taken.
Animals are not permitted to visit or live in the residence halls, with the exception of animals approved by the Accessible Education Center (AEC). Residents must provide AEC with documentation from a doctor as to the resident's need for an assistive or emotional support animal.
Residents requesting to have an assistive or emotional support animal living in on-campus housing may be permitted on a case-by-case basis. Assistive or emotional support animals may not reside in University Housing without prior notification, registration and approval. Requests for an assistive or emotional support animal will be evaluated by the AEC. Housing must receive approval from the AEC prior to the resident being accommodated. Once approval has been received by University Housing Services, resident will be required to complete an Assistive Animal Addendum.
Please review your Annual License Agreement Booklet for the entire Pet Policy.


text:
Der Sennhof in Chur war neben der Justizvollzugsanstalt Realta bei Cazis eine der beiden Strafanstalten des Kantons Graubünden. Er liegt am Rande der Altstadt zwischen dem Hof und der Regulakirche.

Gebäude
Der Bau wurde 1603 unter dem Churer Stadtrat und bischöflichem Münzmeister Hans Jacob Wegerich (1562–1606) errichtet. Der Sennhof wurde zuerst als Wohnhaus verwendet sowie zum Teil als Landwirtschafts- und Seife produzierender Industriebetrieb.

Die Strafanstalt
Die Geschichte der Strafanstalt Sennhof beginnt im Jahr 1817, als der junge Kanton Graubünden für etwa 40'000 Schweizer Franken das Grundstück samt Gebäude erwarb, es als Zuchtanstalt umnutzte und einen dort domizilierten Verhörrichter einsetzte. Von 1830 bis zum Abbruch 1963 bestand als Teil des Sennhofs das wegen seiner Platzverhältnisse berüchtigte Gelbe Blockhaus. 1934 wurde der Sennhof umfangreich ausgebaut. Ab 2003 verfügte der Sennhof auch über eine 20 Plätze umfassende Abteilung für Ausschaffungshaft.

Im Frühjahr 2020 wurde der Sennhof endgültig geschlossen. Die verbliebenen Insassen wurden in die neue Justizvollzugsanstalt Cazis Tignez verlegt. Bis Anfang 2023 soll der Umbau des Sennhof-Areals zu einem Wohnkomplex mit Gewerbeflächen, Ateliers, Museum, Restaurant und einem Hostel abgeschlossen sein.

Weblinks
 So geht es mit dem Sennhof weiter. Artikel der Südostschweiz vom 31. Oktober 2019

Einzelnachweise

Bauwerk in Chur
Gefängnis (Schweiz)
Ehemaliges Gefängnis


This has brought about tension in the school, however, the institution's electoral committee is trying everything in her power to make sure the election turns out to be a peaceful and a successful one. The aspirants have been addressed to shun any form of violence. Several measures have been put in place by the Management of the institution to record a successful SUG Election.


The most important economic events for the day are concentrated around GMT13:30 – the U.S. non-farm payrolls (185K exp., 74K prev.) and the U.S. unemployment (6.7% exp., 6.7 prev.).
EUR/USD – the yesterday's lack of more easing on behalf of the ECB lead to a rise of the euro for the day. Today the dollar seems to be gaining strength again. Currently the pair trades around $1.356 after going to as high as $1.3618 yesterday. Technically the direction is still negative on the daily graph where the MACD is in the negative area and the pair did not manage to go steady beyond the 30 moving average.
AUD/USD – the bullish movement seems to be a bit exhausted for the moment. On the 4h graph (see below) there is a bearish formation which could lead to a temporary stop of the increase similarly to the situation in AUD/NZD. Both currency pairs still look positive on the daily graphs though.
EUR/CHF – the pair tried to advance but was stopped close to the 38.2 Fibo retracement level. Still negative on the daily. A possibility for a bullish divergence on the weekly graph but we'll have to see if it is confirmed during the next several days.


After taking control of Mosul, Iraq's largest city last month, the extreme al Qaeda splinter group ISIS (Islamic State of Iraq and al-Shams) are continuing their New Iraqi Order. This time, instead of telling women to stay inside, they are covering the faces of mannequins.
According to NBC News, Mosul shop keepers say they've been instructed to drape the heads of not only their female mannequins but also their male mannequins in black cloth. Some ISIS members feel this demonstration strictly upholds Shariah law, which "forbids statues or artwork depicting the human form."
It may seem extreme, but just last month the same group advised women to "dress decently and wear wide clothes. Only go out if needed." What's the definition of if need be? Groceries? Air? ISIS also banned drugs, alcohol, cigarettes and public gatherings, probably where people would do all of the above. But hey, prohibition worked so well in America that time!


The Cross has beautiful oval glass stones in red, blue, and green with sparking clear stones at each point of the cross. This piece is like new. This is a vintage piece and would be a true treasure to any collector and of course gorgeous to wear now. The item "TRIFARI Vintage Maltese Cross Brooch Pin Pendant Glass Stones Rhinestones NEW" is in sale since Wednesday, December 19, 2018. This item is in the category "Jewelry & Watches\Vintage & Antique Jewelry\Costume\Designer, Signed\Pins, Brooches". The seller is "simplyyaya" and is located in Urbanna, Virginia. This item can be shipped to United States.


text:
Ten more arrested over KDF murder
By Cyrus Ombati
Ten more suspects have been arrested in connection with the killing of three Kenya Defense Forces soldiers in Garissa in a police operation.
The ten have also been linked to the killing of six other police officers who have been killed in ambushes in Garissa Town in the past three months.
They were arrested in the on-going police hunt for terror suspects in the region. Police have been combing the town in search for suspects who have been linked to a series of attacks in Garissa and its environs.
Officials holding the suspects say some have confessed they are part of a larger network that had been sent from Somalia by the Al-Shabaab militants to "deal" with security agents over the military operations in their country.
"They are part of a larger gang of foreigners who have been behind these attacks," said an officer aware of the case.
Another official said the suspects were named by an accomplice arrested on Saturday over the killing of the three soldiers on November 19.
Ibrahim Jelle Belle was charged in court however not required to plead to the murder charges as the prosecution pleaded for more time to conclude investigations.
Belle was arraigned before Principal Magistrate John Onyiego who remanded him at the Garissa police station.
The prosecution said they were likely to also charge him with the murder of the three police officers killed prior to the murder of the murder of the soldiers.
While applying for more time, the prosecution told the court that two soldiers who survived the shootings had positively identified the suspect as part of the gunmen who attacked them.
The suspect who is a Somali national was arrested on Saturday in Hulugho area near the Somalia-Kenya border as he tried to flee, police said.
But Belle said he was Kenyan from Ijara District and had never committed any crime against any government officer.
He told the Magistrate that he read politics and demanded that two chiefs and a police sergeant in Hulugho where he was arrested be available during his prosecution.
The case will be mentioned on December 9th at the Garissa High court.
Other sources said the suspect is among many Somalis who illegally obtained Kenyan documents and that a major investigation into the incident and others has been launched.
The killing of the soldiers prompted a police operation and sparked protests and riots in the town leaving property being destroyed.
A parliamentary group is investigating the incident and reaction to the same.
KDF has denied claims they harassed residents of Garissa and burnt property while hunting down assailants who had killed the soldiers.
Portland suspect: I was drunk, but I didn't rape - Portland Press Herald


If you are a homeowner on the look-out for a licensed electrician in Marysville, WA, give Beacon Plumbing a call. We are full-service independent electrical contractors equipped to handle practically any job involving electrical wiring and electrical fittings.
We send out a duly certified, experienced and diligent residential electrician to your Marysville home. Our licensed electrician serves you with work that is efficient, accurate, neat and enduring. You can count on our residential electrician to take all precautions required to complete an incident-free job and ensure successful electrical installation, repair, remodel, upgrade or maintenance.
Even a single slipup while working on the electrical system can result in unpleasant scenarios like blackouts and fixture/appliance damage. Sometimes, the outcome can be tragic like fatal electric shock or home fire.
It is best that you avoid ringing up just about any of the neighborhood electricians and let our licensed electrician serve you in Marysville. Our residential electrician is suitably qualified to do the job and can be relied upon to get it done without any mistake, damage or compromise on quality.
When it comes to electrical inspection or electrical troubleshooting, our services as a residential electrician are second to none. Every licensed electrician employed by us is an expert at electric service and repair. Use of top-line tools, top-grade wiring, switches and outlets, etc. and flawless workmanship are a given on all the major and minor jobs that our licensed electrician take up in Marysville.
Need a residential electrician in Marysville? Call Beacon Plumbing at (206) 452-1932.


text:
Fred Martini es un deportista austríaco que compitió en skeleton. Ganó una medalla de bronce en el Campeonato Europeo de Skeleton de 1984.

Palmarés internacional

Referencias

Martini, Fred


text:
Q: Sonar CeWorkerRunnableImpl - Failed to execute task AVKxZzs3XOSwYL28ZL1g Facing nullpointerexception while integrating jenkins(1.646) and sonar(5.2).
I am trigerring jenkins job for sonar scan, which is completing sucessfully.
INFO] Analysis reports generated in 17987ms, dir size=9 MB
[INFO] Analysis reports compressed in 21186ms, zip size=3 MB
[INFO] Analysis reports sent to server in 1029ms
[INFO] ANALYSIS SUCCESSFUL, you can browse http://10.72.118.177:9000/dashboard/index/com.barclaycard.bpay.core:bPay_Core
[INFO] Note that you will be able to access the updated dashboard once the server has processed the submitted analysis report.
[INFO] More about the report processing at http://10.72.118.177:9000/api/ce/task?id=AVKxZzs3XOSwYL28ZL1g

However when I look at the status on sonar it I'm getting the below error -
016.02.05 12:28:52 ERROR [o.s.s.c.t.CeWorkerRunnableImpl] Failed to execute task AVKxZzs3XOSwYL28ZL1g
java.lang.NullPointerException: null key in entry: null=org.sonar.server.computation.sqale.SqaleRatingSettings$LanguageSpecificConfiguration@1a17df9
    at com.google.common.collect.CollectPreconditions.checkEntryNotNull(CollectPreconditions.java:31) ~[guava-17.0.jar:na]
    at com.google.common.collect.ImmutableMap.entryOf(ImmutableMap.java:135) ~[guava-17.0.jar:na]
    at com.google.common.collect.ImmutableMap$Builder.put(ImmutableMap.java:206) ~[guava-17.0.jar:na]
    at org.sonar.server.computation.sqale.SqaleRatingSettings.buildLanguageSpecificConfigurationByLanguageKey(SqaleRatingSettings.java:54) ~[sonar-server-5.2.jar:na]
    at org.sonar.server.computation.sqale.SqaleRatingSettings.<init>(SqaleRatingSettings.java:46) ~[sonar-server-5.2.jar:na]
    at sun.reflect.NativeConstructorAccessorImpl.newInstance0(Native Method) ~[na:1.8.0_60]
    at sun.reflect.NativeConstructorAccessorImpl.newInstance(Unknown Source) ~[na:1.8.0_60]
    at sun.reflect.DelegatingConstructorAccessorImpl.newInstance(Unknown Source) ~[na:1.8.0_60]
    at java.lang.reflect.Constructor.newInstance(Unknown Source) ~[na:1.8.0_60]
    at org.picocontainer.injectors.AbstractInjector.newInstance(AbstractInjector.java:145) ~[picocontainer-2.15.jar:na]
    at org.picocontainer.injectors.ConstructorInjector$1.run(ConstructorInjector.java:342) ~[picocontainer-2.15.jar:na]
    at org.picocontainer.injectors.AbstractInjector$ThreadLocalCyclicDependencyGuard.observe(AbstractInjector.java:270) ~[picocontainer-2.15.jar:na]
    at org.picocontainer.injectors.ConstructorInjector.getComponentInstance(ConstructorInjector.java:364) ~[picocontainer-2.15.jar:na]
    at org.picocontainer.injectors.AbstractInjectionFactory$LifecycleAdapter.getComponentInstance(AbstractInjectionFactory.java:56) ~[picocontainer-2.15.jar:na]
    at org.picocontainer.behaviors.AbstractBehavior.getComponentInstance(AbstractBehavior.java:64) ~[picocontainer-2.15.jar:na]
    at org.picocontainer.behaviors.Stored.getComponentInstance(Stored.java:91) ~[picocontainer-2.15.jar:na]
    at org.picocontainer.DefaultPicoContainer.getInstance(DefaultPicoContainer.java:699) ~[picocontainer-2.15.jar:na]
    at org.picocontainer.DefaultPicoContainer.getComponent(DefaultPicoContainer.java:647) ~[picocontainer-2.15.jar:na]
    at org.picocontainer.DefaultPicoContainer.getComponent(DefaultPicoContainer.java:632) ~[picocontainer-2.15.jar:na]

A: The stacktrace reports an unexpected corruption of the configuration of the debt by language.
Please look at the admin page located in Administration > Technical Debt.
In the section "Language specific parameters", it's very likely you have an empty language key such as below:

Ticket SONAR-7154 has been created to better handle this.

redpajama_set_name: RedPajamaStackExchange


text:
kol neshama
giving creative expression to the voice of the soul
Donate to Kol Neshama
Buy DVDs & CDs
Chabad.org: Female-Only Movie Screens in 11 Cities During Passover Holiday
by Sara Trappler Spielman
Robin Garbose, the Jewish director who earned headlines for her female-only screenings, is at it again, scheduling showings of her latest film in 11 American cities over the upcoming Passover holiday.
Crowds of fans turned up for the premiere of "The Heart That Sings" last month at the Jewish Children's Museum, headquarters of the Chabad-Lubavitch youth organization Tzivos Hashem, in the Crown Heights section of Brooklyn, N.Y. They filled every last seat – a total of 700 – in the venue, cheering as Garbose's name appeared on the screen.
(The premiere in Los Angeles the day before also sold out, attracting 600 mothers and daughters at the city's Museum of Tolerance.)
Many students from Beth Rivkah High School, the largest Chabad girls' school in Crown Heights, arrived at the children's museum eager to see friends and classmates appear in the film.
Fourteen-year-old Malka Kugel, a Beth Rivkah student, plays one of the mean girls in the musical, which tells the story of a young Holocaust survivor named Miriam who spends a 1950s summer as a drama director at a camp in the Catskills Mountains of New York. At first, the campers take advantage of Miriam's quiet, broken spirit, but in the end, they transform each other in a magnificent way.
Kugel, who received no training before attending Garbose's Kol Neshama program last summer, felt "like a professional actress" after merely seven weeks of study.
"When we were filming, it was magical," she said. "Everything we were taught came together."
Garbose's first film, "A Light for Greytowers," was released three years ago and proved to be a hit, especially among religious teenage girls. Set at a Victorian orphanage in England, it revolves around a young girl, also named Miriam, who is separated from her parents after escaping Czarist Russia. She fights to observe her Judaism in the orphanage under the rule of a cruel matron.
"Greytowers" screened to 50,000 women in cities across the United States, Canada and Israel, including at major venues such as the Atlanta Jewish Film Festival, the Jewish Eye World Festival in Ashkelon, and the Jerusalem and Tel Aviv Cinematiques. It has been available on DVD since Chanukah and has already seen 5,000 sales.
With "The Heart That Sings," Garbose anticipates even greater numbers. She has already received about eight solicitations from film festivals and believes this film can reach a broader audience with its Holocaust theme and cinematic quality.
"There's something so compelling about the character of Miriam," Garbose said at the movie's premiere.
"We're not used to seeing a beautiful young woman with a number on her arm," Garbose continued. "It makes you experience the Holocaust in a new and emotional way."
Armed with now two films, Garbose is creating a new genre in the world of women's entertainment. Both are movie musicals featuring religious actresses, singers and dancers trained at Kol Neshama, her performing arts conservatory in Los Angeles. Because of Jewish laws of modesty that allow women to sing and dance only for other women, opportunities are sparse for talented girls and women wishing to perform professionally.
"We broke the ice with Greytowers" by ensuring the film screen to female-only audiences, explained Garbose.
Yocheved Daphna, whose daughter Golda attended Kol Neshama and acts in "The Heart That Sings" cried through the movie's premiere.
"I'm so glad they have an outlet in a proper environment for actresses, singers and dancers," she enthused. "To have a first-class project with a professional to fine tune their skills" is unique.
Garbose discovered Rivka Siegel, who played the lead role in both "Greytowers" and "The Heart That Sings", when she was acting in her eighth-grade play years ago. Upon seeing such talent, Garbose was motivated to soon open Kol Neshama.
Garbose said she "wrote the ["Heart"] script with Rivka in mind because of her emotional depth."
Siegel said that Garbose often gives her roles of powerful women and she loves that.
"I loved the character [of Miriam in "The Heart that Sings"]," she stated at the premiere. "She had many layers, and is an amazing character."
Last summer, girls from all over the United States who attended the Kol Neshama program rehearsed for four weeks and then shot the movie under Garbose's direction in only 18 days.
Garbose hired a professional Hollywood crew to shoot at several locations in and around L.A.: a beautiful shooting ranch on a far-out valley for exterior shots, an RV park for interiors and downtown for some Manhattan-style architecture. She rented period cars from the late 1940s and found Brooklyn props to properly replicate that era.
Made on a small budget by Hollywood standards – the film cost $350,000 to produce – Garbose attained completion funds through a clever online fundraising campaign on Kickstarter.com. Using the number 18 – the numerical value for the Hebrew word meaning "life"- Garbose reached her goal of raising $18,000 through incentive awards for backers who pledged money.
A director for 27 years, Garbose said: "It's time for authentic Jewish content to emerge in the world of film."
"Heart" invited to the Jerusalem Film Festival!
Big news! The Israel premiere of "The Heart that Sings" will be a part of the 13th Jerusalem Jewish ...
Jerusalem Post: Women in the Picture
by Hannah Brown Robin Garbose's groundbreaking new film, 'The Heart that Sings,' is a movie musi...
The Jewish Daily Forward: The Arty Semite
By Sarah Trappler Spielman Orthodox director Robin Garbose is at it again. First she made history...
Chabad.org: Atlanta Jewish Film Festival Engages Female-Only Audiences
by Libby Herz One of the most-widely attended Jewish film festivals in the United States shined a s...
Sara in Miami says:
I saw the Heart that Sings with my daughter Chana last night at a real movie theater. It was so much fun. We loved it!
We are looking forward to more great movies from Robin and her students in the future, IY"H. Everyone loved Judy in the movie. Judy did my make-up for my wedding. I recognized her right away. When a cd of the songs is available, and also a dvd, I'd like to buy it. I loved the music.
Margalit says:
First of all, I wanted to congratulate and thank you for putting out
such a beautiful, inspiring, touching and professional film for the
frum female population!
I really enjoyed it!
I was also wondering if you are selling a soundtrack from the film. If
so, I would be interested in finding out how to obtain one!
Year-End Message from Robin Garbose: Make a Donation to Kol Neshama
Opportunities for talented Jewish girls to develop and express their creative gifts through Kol Nesh...
Camp Bnos Yisrael DVD Series - Watch the Trailer and Order Now!
Camp Bnos Yisrael, a new DVD series for girls by acclaimed filmmaker Robin Garbose, will be availabl...
Haaretz: Tel Aviv Cinematheque tries to bar men from screening of film by ultra-Orthodox director
Cinematheque program says the movie creators 'politely request that only women and girls attend the...
Yaldah Magazine: Interview with Rivka Siegel
by Yehudis Keller, age 15, NY Hi Rivka! We are so glad for this opportunity to talk to you. Many of...
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text:
Q: GET http://localhost:4000/ 404 (Not Found) Refused to load the image 'http://localhost:4000/favicon.ico' because it violates the following Content Security Policy directive: "default-src 'none'". Note that 'img-src' was not explicitly set, so 'default-src' is used as a fallback.
I have tried to rebuild the server using different port also, but issue persist the same.
Any solution???
"dependencies": {
"express": "^4.17.1",
"socket.io": "^2.3.0"
}

A: The server appears to be sending a CSP (Content Security Policy) header and as the error message says, it has not set any 'img-src' so it falls back to 'default-src' which is None.
https://developer.mozilla.org/en-US/docs/Web/HTTP/Headers/Content-Security-Policy/default-src
If you are expecting the server to provide images, see if you can set the used CSP, how to do this is dependent on which webserver you use, so a little more information is needed to help with that.


Pomelo is Corner 53's citrus explosion. They go to great lengths to pack this full of fresh Pink Grapefruit, Pear and Liquorice. Not feeling that just using the peel was enough, they also pass the vapour through a layer of the juicy centres to really capture the vibrant flavour.
Corner Fifty Three is a distilling project 5 years in the making. Bearded bartender Tom Rudman, having spent 4 years mixing cocktails, just couldn't find his perfect gin. A family move to the heart of the South Downs and with the 'put up or shut up' cries of select friends ringing in his ears, Tom set about persuading his parents that Copper Pot Stills make fantastic garden ornaments.
In 2016 Corner Fifty Three was born. Over the following bitterly cold winter months, with the help of his father and a rapidly dwindling savings account, a crooked but very sturdy home was built. The distillery was finished just in time for the arrival of Rosie and Angus, our much loved, hand built, copper stills.
With Rosie and Angus moved in, the rest of the team was recruited from the ranks of the 'put up or shut up' brigade. Richard Cope, Cris Bowyer and Jamie Williamson all coming on board to help fill the gaps. Together they are building Corner Fifty Three around Tom's idea of independence, creativity and passion (and strong facial hair).


Potassium iodate (KIO3) does not affect gold in any way.
but usually not in the time necessary to dissolve thin films of gold.
Subject: [mems-talk] Potassium iodate effect on gold?
does the gold perhaps reduce the iodate?
of MEMS and Nanotechnology design and fabrication services.


Manager and leader are terms that don't necessarily always go hand in hand. Although there's no doubt the two are linked and often overlap, there are some important elements of leadership that the title of 'manager' just doesn't cover.
Becoming a strong leader means mastering the art of communication. To reach the level of manager, you'll have no doubt demonstrated some level of talent for this; but to set yourself apart as a leader, you need to make sure truly impactful communication is at the heart of everything you do.
Strong team leadership requires not just regular, but shrewd communication. While transparency on developments within the company is valuable for team morale and development, a lot can be said for possessing astute judgement about what you share to keep morale buoyed and your team driven towards success.
As an integral part of your team, you need to be an ever present member of the team, a presence at the very heart of everything they do.
Depending on the demands of your job, there will always be instances when you're not physically around, but it's imperative your team knows you are available and approachable so they know they can come to you when it matters. Set up regular one-on-one's and catch ups with your team, invest real time in their development and more than anything, nurture a culture of openness and approachability that fosters trust and respect throughout the team.
Everyone has their bad days - days when they feel tired, unmotivated, distracted or less than 100%. Apart from the true leader, of course.
Your team relies on you to be measured and consistent in your role. It means expressly setting a standard that your team can trust and lean on. That includes everything from the way you address disciplinary matters through to backing them up on internal issues. Your team needs to know that they can trust you and know what to expect from you to get the best out of their performance.
Your team will look to you to be the person who's on the ball at all times.
From meetings to workloads and, team projects, you need to know what's going on, who's doing what and how to approach the next steps before anyone else does. Not only that, but if operations run smoothly and everyone knows their responsibilities, then you also need to create solid guidelines for others to follow. This then makes another key aspect of team leadership easier to introduce: delegation.
Delegation is one of those tasks that anyone can do. But effective and impactful delegation is an art, and one that only the most effective team leaders can learn to master.
Delegating work doesn't mean passing on the stuff you don't want to do - it's about lightening your own workload, making sure the right people are on with the right tasks and empowering team members at the same time. Becoming a leader at work means you have time pressures in other areas, so even if your natural inclination is to take on everything placed in front of you, it's just not possible, nor beneficial, to you or your team. By delegating new work to others, you give them the opportunity to expand their portfolio, gain new experiences and grow.
As you delegate, aim to lead from the front. By coaching others through new tasks and experiences, sharing opportunities within your team and working hard yourself, you'll enhance your position as a true leader and help your team become more productive and effective along the way.
As a team leader, you need to command an impressive level of knowledge and, carry that off with confidence.
The two properties are linked - if you know your stuff when it comes to your industry, you'll feel confident in your performance and your expertise will influence your team. It goes without saying that any credible leader needs to be respected by his or her team; if that respect is missing, it can seriously hamper your chances of being a good team leader.
One of the key things that sets leaders apart from managers is the ability to innovate.
By bringing new ideas to processes and looking out for new ways to improve the way your team works, you lead by example and encourage others to find new ways to get tasks done. You'll also inspire those around you to work harder and instil a practice of looking for constant improvement and development opportunities, the driving force of success.
Leadership may be very different from management, but it's also something that can be learnt and developed over time. Set the foundations for an excellent future as a leader, or brush up on your skills with the right training course. Pareto Law offer a range of leadership training courses that can help you on your way to team management greatness.
For a more experienced manager, our Leadership and Management Training Course will equip you with the skills and strategic approaches to motivate and mentor you team. If you've got your eyes set on greatness, but are still starting off in your career, the Team Leader Level 3 Apprenticeship will build and develop on your natural attributes.


Abstract : Chemical warfare agents present an obvious risk to individuals suffering acute exposure, but they may also present long-term environmental or occupational health hazards for workers in operations involving these chemical agents. Occupational health standards have not been established for Lewisite (L) dichloro(2-chlorovinyl)arsine a potent vesicant which reacts with sulfhydryl groups of proteins through its arsenic group. The destruction of current stockpiles of Lewisite by the U.S. Army in the near future could create additional environmental and occupational risk. To establish a database for setting environmental and occupational standards, we conducted studies to evaluate the toxicity, mutagenicity, and reproductive effects of Lewisite using in vitro and in vivo study systems. The cytotoxic, clastogenic mutagenic effects of Lewisite in Chinese hamster ovary cells were investigated and are described in this report. One mutation assay and two cytogenetic assays were used in this study. The mutation assay utilized the hypoxanthine-guanine phyosphoribosyl transferase (HGPRT) locus (6-thioguanine resistance). The two cytogenetic analyses were chromosomal aberration analysis, measurement of chromosome damage, and sister chromatid exchange (SCE), a measurement of chromosome rearrangement. The CHO cells were exposed in the test system for 1 hour, then washed and cultured for an additional 20-30 hours, depending on the assay to be used. The total number of mutant colonies were determined and the mutation frequency was calculated. Chromosome aberrations were scored using 100 metaphases per dose and SCE per cell were calculated.


text:
package sl.paket.sandbox;

public class Myprog {
  public static void main(String[] args) {
  hello("Sergei");

      Square s= new Square(7);

      Rectangle r = new Rectangle(6,7);

      System.out.println("Area rectangle ="+ r.area1());
      System.out.println("Area square ="+ s.area2());

  }
    public static void hello (String whoUare) {
   System.out.println("Hello," + whoUare+ "!");

 }

}


text:
MoradNET
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Motorola offers an extensive range of products for your business, including voice and data radios, broadband wireless transmitters, headsets and much more.
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We work with your team from start to finish, ensuring the most efficient integration of new technologies into your existing network infrastructure.
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Communicating Safely While Socially Distancing
Digital Two-Way Radios / By alazarsemere
Have questions about communicating safely?
2020's COVID-19 pandemic is forcing businesses to re-evaluate the way they operate, especially when it comes to communicating. Social distancing has gone from being a foreign term to everyday language in a matter of weeks, and the practice may continue for some time to come. That said, the need for communication in Alberta's workplaces isn't going away. That means new strategies will have to be found.
Email, smartphones, and video conferencing are all effective options that work well in office environments, but for businesses that require employees to constantly be on their feet and on the move, solutions like two-way radios are extremely effective. Their transmit range is more than enough to accommodate social distancing precautions and providing radios for each team member means they won't have to share, mitigating the risk of sharing any germs.
For more information on how to stay safe during the COVID 19 pandemic, check with Alberta Health or Health Canada. For information on how to communicate effectively and safely, Contact Us, or keep reading.
Communicating Safely with Two-Way Radios
Industrial Environments
Clients working in the resource sector up near Fort McMurray have come to us looking for ways to utilize existing infrastructure to incorporate social distancing into their day-to-day communications. One example is the face to face meetings held at the start of a shift.
In a time where social distancing is quickly becoming the new norm, this simply isn't possible if safety is going to be maintained. The challenges are only compounded by the fact that many of these clients work, and conduct these face to face meetings, in high-noise environments, which can make hearing both conversation and radio calls difficult.
The solution? We have been advising them to pair their radios with headsets that offer noise cancellation. These accessories will filter out much of the background noise common to industrial environments, and the range of the radios is more than enough to accommodate the need for social distancing.
More and more grocery stores are exercising additional precautions to limit the number of people in their stores. If you walk up to any grocery store in Edmonton, Calgary, or Fort McMurray, you may see a lineup outside the door; that's because business owners are trying to keep their customers safe.
If you manage a retail business, you know that communication is key to managing this queue of shoppers, and it's likely that you'll end up using security guards. Equipping them with handheld radios will make their jobs considerably easier. With these radios, the guard stationed at the exit could inform the guard at the entrance of how many shoppers had just let, and how many could be allowed in.
If the supervisors were equipped with radios, they could communicate with the guards as well, and stay informed of foot traffic numbers or of any incidents happening outside the store.
The radios you end up using don't have to be fancy or complicated – they just need to provide loud, clear audio. With those tools in hand, it will be much easier to enforce the safety measures you're already taking to protect customers and staff alike.
Callboxes
You've used a callbox before. It's basically an intercom system that allows you to speak with someone on the other side of a secured entrance – apartment buildings use them all the time. Business operators and service providers can use this solution, as well.
For example, imagine you run a medical clinic where blood samples are taken. Alberta legislation, and your own desire to keep patients safe, may require you to limit the number of people in your clinic. A callbox, and an access control device, could help accomplish this. People wanting to gain entry would have to 'buzz in' and announce themselves. If there was the capacity to accommodate them, they could be allowed entry. If not, your staff could use the intercom function to inform them they had to wait.
While a security guard may be required to ensure patients don't try and enter all at once when the door is open, callboxes can really help regulate entry and the overall number of people in a shared space.
Don't Share
If you plan on using radios, be sure everyone has their own. The same goes for accessories, like headsets and speaker mics. Germs can live on the surface of this equipment and sharing radios could unintentionally lead to you sharing germs, too.
Consider using individual battery chargers, as well. While gang chargers make it easier to organize your batteries, they can also become a shared space of sorts.
Regularly Clean and Disinfect Your Equipment
Radios and radio accessories get dirty simply because of the work environments they are used in. But with the sanitization of equipment now being a priority as well, more attention should be paid to this bit of routine maintenance.
It's always best to rely on trained radio technicians to do this job, even if it seems simple. Use the wrong chemical, like bleach, and you could damage the radio. Use water or sprays directly on the radio, and the fluid could enter the housing, damaging the inner components. If you don't have a trained technician on staff, talk to your radio supplier about a cleaning plan to ensure your gear is regularly cleaned and sanitized.
COVID-19 has heightened the need to stay safe and changing the way we communicate is going to be a factor in fulfilling that need. The team at Tridon Communications is here to help you get there. We will work with you to build the solution that meets your communication needs, safety requirements, and budget in these trying times.
If you have any questions, Contact Us. We're here to help.
Tridon is a full solution Telecom Systems Integrator with CSA certification and licensed by APEGA. Our Engineering, Service and Tower Divisions collaborate with customers to build engineered solutions including communications systems design, tower inspections, and co-location, wireless broadband, fiber optic cabling, site security, and two-way radio communication.
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