PLoS Pathog. 2012;8(3):e1002529. doi: 10.1371/journal.ppat.1002529. Epub 2012 Mar 8.
Whole genome deep sequencing of HIV-1 reveals the impact of early minor variants upon immune recognition during acute infection.
Henn MR1, Boutwell CL, Charlebois P, Lennon NJ, Power KA, Macalalad AR, Berlin AM, Malboeuf CM, Ryan EM, Gnerre S, Zody MC, Erlich RL, Green LM, Berical A, Wang Y, Casali M, Streeck H, Bloom AK, Dudek T, Tully D, Newman R, Axten KL, Gladden AD, Battis L, Kemper M, Zeng Q, Shea TP, Gujja S, Zedlack C, Gasser O, Brander C, Hess C, Günthard HF, Brumme ZL, Brumme CJ, Bazner S, Rychert J, Tinsley JP, Mayer KH, Rosenberg E, Pereyra F, Levin JZ, Young SK, Jessen H, Altfeld M, Birren BW, Walker BD, Allen TM.
Deep sequencing technologies have the potential to transform the study of highly variable viral pathogens by providing a rapid and cost-effective approach to sensitively characterize rapidly evolving viral quasispecies. Here, we report on a high-throughput whole HIV-1 genome deep sequencing platform that combines 454 pyrosequencing with novel assembly and variant detection algorithms. In one subject we combined these genetic data with detailed immunological analyses to comprehensively evaluate viral evolution and immune escape during the acute phase of HIV-1 infection. The majority of early, low frequency mutations represented viral adaptation to host CD8+ T cell responses, evidence of strong immune selection pressure occurring during the early decline from peak viremia. CD8+ T cell responses capable of recognizing these low frequency escape variants coincided with the selection and evolution of more effective secondary HLA-anchor escape mutations. Frequent, and in some cases rapid, reversion of transmitted mutations was also observed across the viral genome. When located within restricted CD8 epitopes these low frequency reverting mutations were sufficient to prime de novo responses to these epitopes, again illustrating the capacity of the immune response to recognize and respond to low frequency variants. More importantly, rapid viral escape from the most immunodominant CD8+ T cell responses coincided with plateauing of the initial viral load decline in this subject, suggestive of a potential link between maintenance of effective, dominant CD8 responses and the degree of early viremia reduction. We conclude that the early control of HIV-1 replication by immunodominant CD8+ T cell responses may be substantially influenced by rapid, low frequency viral adaptations not detected by conventional sequencing approaches, which warrants further investigation. These data support the critical need for vaccine-induced CD8+ T cell responses to target more highly constrained regions of the virus in order to ensure the maintenance of immunodominant CD8 responses and the sustained decline of early viremia.
PCR amplification strategy and performance of novel assembly, read alignment, and variant detection algorithms.
(A) PCR amplification strategy using four ∼3.2 kb amplicons spanning gag through nef of the HIV-1 genome. Amplicons were then pooled, sheared, barcoded by patient or time point, and batched for library construction and single-molecule 454 pyrosequencing. (B) AssembleViral454 v1.0 outperforms other algorithms in its ability to assemble de novo continuous consensus contigs that span the complete target region. Results are shown for 67 acute, chronic, or controller patient samples that had successful amplification of all four amplicons and at least 10-fold sequence coverage (sequencing reads per site) across >70% of the target genome. Black lines denote the mean score for each assembler, red line the median, red box ends the 25th and 75th quantiles, and red box whiskers the upper and lower quartiles plus/minus 1.5 times the interquartile range, respectively. (C) ReadClean454 v1.0 corrects for read alignment errors due to various sequence error modes and significantly reduces process error rate. Results shown are for virus from two infectious clones, NL43 (WT) and NL43 (RKLM) containing two point mutations in Gag , sequenced independently to 417- and 189-fold average coverage, respectively. Errors are defined as base calls or InDels that differ from the assembled consensus at a given position, and the read error rate is the total number of errors per total number of NQS passing bases interrogated. Percentage of reads on which a correction was made at each step are shown in parentheses. A final average process error rate of 0.5×10−4 was achieved based on both infectious clones. (D) V-Phaser v1.0, utilizes phasing information to identify a variant pair found in 1.0% of the reads covering both loci when there are 200 such reads; without phase, a three-fold increase in coverage is required to achieve the same 1.0% detection threshold. A variant at a frequency of 0.1% can be detected when phased coverage is 2999-fold.
Comparison of sequence variant quantification by 454 deep sequencing and by PCR cloning/sequencing.
Orthogonal regression of variant frequency estimates obtained by 454 and clonal sequence data across the highly variable 1544 nucleotide region spanning Vif to Tat in subject 9213 (slope = 1.01; 95% CI, 0.73 to 1.40).
Clinical course and whole genome deep sequence coverage for subject 9213.
(A) Clinical course of infection in subject 9213 shown as days post-presentation. Plasma viral load (copies/ml) is shown in red and CD4+ T cell count (cells/ml) in blue. Estimated acute/early Fiebig stages are shown and arrows indicate time points sequenced on the deep sequencing platform. (B) High-quality sequencing reads per site across the HIV-1 genome for subject 9213 at six time points (days post presentation). Reads are aligned to the consensus assembly of their respective time point using Mosaik v1.0 (Table S9 in ) and coverage (sequencing reads per site) calculated from bases that pass the defined Neighbor Quality Standard (NQS, see Supplementary Methods in ) . PCR amplicon locations are denoted by horizontal bars under the x-axis.
Rapidly expanding sequence diversity during HIV-1 infection.
Heat maps illustrate sites exhibiting amino acid sequence diversity at days 0, 3, 59, 165, 476 and 1543 post-presentation. Plotted is the percentage of amino acid diversity at each position with respect to the dominant baseline (day 0) amino acid residue. All 3174 amino acids of HIV-1 are represented, with the first amino acid of Gag located in the top left corner of the grid and the last amino acid of Nef located in the bottom right corner. Completely conserved residues are dark blue, low-level variant residues (<10% divergent from baseline) are light blue, moderately variable residues (10–50%) in orange, and highly variant residues (>50%) in red. (A) 0 days p.p., (B) 3 days p.p., (C) 59 days p.p., (D) 165 days p.p., (E) 476 days p.p., (F) 1543 days p.p..
Limited evolution in the HIV-1 proteome prior to establishment of viral set point.
Sequence diversity is plotted for all evolving codons in each HIV-1 protein as the percent of sequences with an amino acid residue different from the dominant baseline residue. Colored lines denote individual evolving amino acid residues within each protein. The time of infection prior to the establishment of viral set point (day 165) is highlighted in grey.
Cellular immune responses drive early low-frequency quasispecies diversity.
(A) For each protein, the average frequency of non-dominant baseline residues of positions within the 19 described CD8 epitopes restricted by subject 9213's HLA alleles (left) and outside of the 19 described epitopes (right) is plotted for each time point sequenced. Colored lines denote the proteins for which diversity was substantially higher inside of CD8 epitopes versus outside CD8 epitopes. (B) To determine rates of viral escape for each epitope escape mutations were defined as any amino acid substitution within the epitope. Symbols denote the cumulative observed frequency of all escape mutations, and lines depict the best fit by non-linear regression of the observed frequency data to the CTL escape model of Asquith et al. . Open symbols and dashed lines denote epitopes for which evolution was consistent with reversion. Black symbols and dotted lines denote epitopes for which there was no evidence of escape. CD8 responses against each epitope are shown in parentheses in the legend and were measured by IFN-gamma Elispot assay (Spot Forming Cells/Mill PBMC (SFC)). (C) Frequency of wild-type (black) and variant (red) haplotypes of the Vif B38-WI9 epitope and flanking regions over time. Shown at the top is the clade B consensus sequence for reference. (D) Frequency of wild-type (black) and variant (red) haplotypes of the Nef A24-RW8 B38-WI9 epitope and flanking regions over time. Blue residues highlight differences between the day 0 transmitted sequence and HIV-1B consensus sequence.
Viral escape from acute and chronic phase CD8+ T cell responses.
Stacked heat-maps illustrate variant codon frequencies over time for each residue of the CD8 epitopes targeted by subject 9213. Shown are epitopes targeted during the acute (Day 59) and chronic (Day 476) phases of HIV-1 infection. The baseline sequence is shown at the top of each epitope, with non-HIV-1B consensus residues highlighted in blue. The magnitude of each response is shown in SFC per million PBMC.
Variant-specific CD8+ T cell Elispot responses.
Elispot responses in Spot Forming Cells (SFC) per million PBMC to wild-type and variant peptides for the two dominant epitopes (A) Vif B38-WI9 (WHLGQGVSI) and (B) Nef A24-RW8 (RYPLTFGW). Bars in black denote responses to clade B consensus epitopes. Bars in red, orange, and pink denote responses to epitopes containing escape variants.
Reversion of transmitted mutations over the course of infection.
(A) Rates of reversion of transmitted mutations within both restricted and unrestricted CD8 epitopes in subject 9213. Reversion was defined as the replacement of a transmitted non-consensus residue by the HIV-1B consensus residue. Symbols denote the observed frequency of viruses expressing the consensus residue and lines depict the best fit by non-linear regression of the observed frequency data to the CTL escape model of Asquith et al. . Closed symbols and solid lines denote epitopes restricted by subject 9213, while open symbols and dashed lines denote epitopes not restricted by 9213. Listed in parentheses are the mutations listed by the consensus residue, HXB2 position, followed by the transmitted mutation, i.e., F197S. (B) Rates of reversion of all transmitted mutations exhibiting sequence variation over the course of infection. Each line represents a different mutation.


text:
Reese è un villaggio degli Stati Uniti d'America, situato nello Stato del Michigan, diviso tra la contea di Saginaw e la contea di Tuscola.

Altri progetti

Collegamenti esterni

Villaggi del Michigan

redpajama_set_name: RedPajamaWikipedia


text:
Niambia brevicauda är en kräftdjursart som beskrevs av Helmut Schmalfuss och Franco Ferrara 1978. Niambia brevicauda ingår i släktet Niambia och familjen myrbogråsuggor. Inga underarter finns listade i Catalogue of Life.

Källor

Myrbogråsuggor
brevicauda


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redpajama_set_name: RedPajamaGithub


text:
UC Cooperative Extension deploys a team of 10 to help farmers practice climate-smart agriculture
Return to Advice to Grow By ... Ask Us!
Scientists are developing climate-smart farming practices, California is offering financial incentives to implement them, and now a group of 10 UC Cooperative Extension climate-smart educators are taking the program to the next level.
To help farmers apply for grants to improve soil quality and enhance irrigation systems, UC Agriculture and Natural Resources partnered with the California Department of Food and Agriculture to put climate smart educators in 10 California communities. The educators are working closely with UCCE advisors to help farmers and ranchers improve soil health, irrigation practices and manure management.
The climate smart programs offered by CDFA and promoted by UC ANR educators are:
State Water Efficiency and Enhancement Program
Healthy Soils Program
Alternative Manure Management Program
The educators provide hands-on assistance to farmers and ranchers through the complex application process, conduct field days with climate-smart farmers, establish demonstration plots to share the practices, and work with farmers who are voluntarily implementing climate-smart farming.
Most of the educators were hired in early 2019, just weeks before the application deadline. They are now gearing up for a second cycle of applications. The state funded 194 projects in 2018, and 217 in 2019.
Each of the educators has a passion for agriculture and the environment, shaped by their upbringing, experiences and education.
"I am interested in carrying out research that focuses on the adoption and economics of climate change best management practices. The practices should help farmers continue their business," said Esther Mosase, climate-smart educator in San Diego County. "I'm interested in seeing policymakers making policies that have a farmer as a focal point. They have been here long, they have been tilling the land, they can also contribute in coming up with better solutions that reduce climate change."
The state is providing incentives for farmers to improve soil health in order to moderate the conditions that are driving global climate change. Improving soil health increases its ability to store carbon and reduce greenhouse gas emissions. Side benefits include improved water infiltration, nutrient cycling and dust control.
Farmers can apply for three-year grants to implement new practices on their farm, such as reducing tillage, growing cover crops and applying compost. Conventional farm practices turn the earth, releasing the stored carbon back into the atmosphere.
UC Cooperative Extension's climate-smart community educators. (Not pictured: Sami Budhathoki)
UCCE's 10 new climate-smart educators are:
Britta Baskerville
UC Cooperative Extension, Mendocino County
blbaskerville@ucanr.edu, (707) 463-4158
Baskerville started college as a theater major in Sacramento, then realized that wouldn't result in a viable career. After suffering from an autoimmune disease tied to microbiome health, she began to understand the important role of the food and agricultural industries in public health. Baskerville earned a bachelor's degree from UC Berkeley that combines sustainable agriculture with the sociological and ecological impacts of agriculture, natural resources conservation and public health.
Last summer, Baskerville served as a program coordinator in an adaptive agriculture learning environment, where she designed two practicum programs for adults. She is considering a career in the food industry.
Climate-smart educator Britta Baskerville, right, makes a point at a UCCE meeting on climate change and environmental justice.
Caddie Bergren
UC Cooperative Extension, Merced County
cmbergren@ucanr.edu, (209) 385-7403
Bergren grew up in a small fishing town on an island in Alaska. She earned a bachelor's degree in earth systems at Stanford University in 2013, and then spent two and a half years in Paraguay as a Peace Corps volunteer. Bergren worked with a women's garden cooperative and with subsistence farmers. She spent the last three years as a community organizer.
"I was so excited to find this job, which combines my interests in working directly with all kinds of people on the intersection of agriculture and climate change," Bergren said. "I've especially enjoyed using my Spanish-language skills to work with traditionally underserved farmers in this area."
Dana Brady
UC Cooperative Extension, Glenn County
dmbrady@ucanr.edu, (530) 517-8187
Brady completed a bachelor's degree in animal science at Chico State University in 2018. She was familiar with UC Cooperative Extension through school and had visited UCCE research sites.
Brady grew up in a farming and ranching family in rural Linden, southeast of Stockton.
"My earliest memory is of my grandfather's farm, where he had an emu, donkey and llama," she said. "I was in 4-H and FFA as long as I can remember."
In addition to working directly with farmers on grant applications, Brady has been helping advisors in Glenn County on research projects and building relationships in the community through workshops and seminars.
"I am also very excited for an upcoming event at an elementary school farm day to present about Climate Smart Agriculture and presenting at some bigger events later this year with a few others in the cohort," Brady said.
Samikshya (Sami) Budhathoki
UC Cooperative Extension, Fresno and Madera counties
sbudhathoki@ucanr.edu, (559) 241-7515
A native of Nepal, Budhathoki traveled to the United States in 2015 to attend college at Fresno State, where she graduated with a bachelor's degree in plant science. During her studies, she completed a weed and salinity management project with professor Anil Shrestha. Budhathoki served as an intern in plant pathology with Bayer Crop Science.
She developed in interest in agriculture because of the industry's importance to society and the world.
"Some people don't get enough to eat even once a day. I wanted to join the effort to end world hunger and food insecurity," Budhathoki said.
Budhathoke said she also is concerned about climate change and welcomes the opportunity to help farmers maintain a sustainable agriculture industry even in the face of climate change.
In the future, she plans to pursue graduate studies in climate change or water management.
UCCE climate-smart educators, left to right, Emily Lovell, Shulamit Shroder and Caddi Bergren presented information about CDFA's climate-smart incentive programs at a field meeting.
Emily Lovell
UC Cooperative Extension, Yolo, Solano and Sacramento counties
ejlovell@ucanr.edu, (530) 405-9777
Lovell grew up in Sacramento and developed an interest in agriculture when she was overcoming a serious illness. She graduated from UC Davis in 2016 with a bachelor's degree in sustainable agriculture and food systems.
"Originally, I didn't want to make money in agriculture," she said. "I wanted to live off the land. I believe farming is a political act and I wanted to help return power to the people through farming and land ownership."
Lovell said she is interested in pursuing a graduate degree in an area that combines community resiliency through localized food systems and economics and, eventually, becoming a crop adviser.
Esther Mosase
enmosase@ucanr.edu, (858) 282-6737
Mosase has a master's degree in agricultural engineering from Botswana College of Agriculture and a doctorate from South Dakota State University in civil engineering. Her master's research focused on water resources, watershed modeling and management.
Raised in a farming family in Botswana, Mosase experienced the impact of climate change firsthand.
"I remember we had drought years, normal years and extremely wet years," she said. "Twenty years ago, it was not uncommon for open water to freeze. But now we get mild winters and very hot summers. Rain-fed agriculture is now a risky enterprise compared to two decades ago."
In addition to helping farmers with the climate-smart farming grant applications, Mosase is helping farmers cope with water quality concerns.
"For instance, one farmer wanted to improve the water quality at the edge of his avocado and citrus farm before it enters the stream. He also wanted to be helped with pools of standing water in the farm that usually affect the health of avocado trees," Mosase said. "We advised him on what to do regarding the standing water, but for the edge of the field treatment, we decided to install bioreactors."
Mosase will help collect field data on the bioreactors' effectiveness and plans to publish the results.
Valerie Perez
UC Cooperative Extension, Santa Cruz County
valperez@ucanr.edu, (831) 763-8028
Perez earned a bachelor's degree in agricultural business at California Polytechnic State University, San Luis Obispo in 2018. She accepted an internship with a large animal veterinarian, and found her passion, she said. In addition to working as a climate smart community educator, Perez is taking prerequisite courses for veterinary school. She hopes her career will lead to conducting research to benefit the meat industry.
"I've always been interested in ways to better agriculture and how our systems could improve, but it wasn't until I received this job that my interest for climate-smart agriculture really peaked," Perez said. "Agriculture is such an important industry, it is vital that we find ways to educate one another on how to better what we have been doing for so many years."
Allison Rowe
UC Cooperative Extension, Ventura County
amrowe@ucanr.edu, (805) 645-1464
Rowe has a bachelor's degree in biology from Colorado College in Colorado Springs and a master's degree from the Bren School of Environmental Science and Management at UC Santa Barbara. Her background and interests focus on the interface of land management and climate change.
"Everyone and everything is interwoven with our food system and yet so much of how we produce food accelerates climate change," Rowe said. "I enjoy being at the interface of science and education, where the rubber meets the road. I wanted to find a role where I could work with people on the ground and implement solutions to climate change while contributing to resilient farming economies."
She said it is encouraging to see that farmers and ranchers are interested in climate-smart agriculture and welcome the technical assistance.
Kristian Salgado
UC Cooperative Extension, Imperial County
kmsalgado@ucanr.edu, (442) 265-7700
Salgado attended San Diego State University, where she earned a bachelor's degree in 2014 with a double major in psychology and environmental studies and minors in counseling and social change. She earned a master's degree in social science at Humboldt State University in 2018.
"My background in agriculture is very broad ranging, from topics relating to public health concerns connected to agriculture production – pesticide drift and agricultural burning – to food insecurity in low-income communities," Salgado said.
Salgado is a native of Calexico, a city located across the border from its sister city, Mexicali, Mexico. Her farming experience centers on urban agriculture.
"Growing food on non-agricultural land has allowed me to learn the technical/scientific processes that go into growing food," she said.
Salgado plans to continue her education in a doctoral program in ethnic studies at UC San Diego, where she can focus on several overlapping areas of interest, including race studies, food justice, sustainable agriculture, climate change, environmental decision-making processes, and participatory action research methodology and practices.
Shulamit Shroder
UC Cooperative Extension, Kern County
sashroder@ucanr.edu, (661) 868-2168
Shroder attended college at the University of Maryland in College Park, earning bachelor's degrees in environmental science and policy and in Spanish language, literature and cultures. She has worked in an agricultural research lab, in the gardens at the University of Maryland and in a nearby organic farm. After graduating in 2016, Shroder volunteered with the Peace Corps in Senegal, West Africa, where she trained farmers on gardening and agroforestry techniques and extended improved varieties of staple crops like beans, corn, millet and sorghum.
"While serving in Senegal, I saw firsthand the effects of desertification and erratic rainfall on the ability of the community to feed itself," she said
Shroder intends to earn a master's degree and continue to research and promote sustainable agriculture techniques.
UCCE climate-smart educator Esther Mosase, left, and UCCE specialist Jeff Mitchell at a field day.
Posted on Tuesday, December 3, 2019 at 9:52 AM
Author: Jeannette E. Warnert
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redpajama_set_name: RedPajamaCommonCrawl


We finally encountered Cheetahs in the Masai Mara. The first night, they were just sleeping from afar and because we wouldn't go offroad (unlike another driver who our guide scared off by saying he had government officials int he car) we didn't really get much. The second day, we had a different guide post a hot air balloon ride and he was on a mission to find the cheetah. We came across one that was clearly hunting, but he went too far out of view (although this guide was itching to go offload but we wouldn't let him). However, after stopping at the loo with a view (too much champagne at the post the hot air balloon brunch), he spotted another one that was much closer to the road for us to get some great photos.
While I never saw the cheetah hunt, I did have one of the most interesting/coolest viewing experiences later that day. A cheetah that had already hunted was strolling towards the bush. The ostriches and impalas were running TOWARDS the cheetah to keep an eye on him. According to our guide, they wanted to make sure he left and running away from him would only signal weakness. Seeing an Ostrich run in fear was fascinating/hilarious.
Kenya: Lions and People, Oh My!


PENNE WITH BROCCOLI AND GOAT CHEESE ΠΑΠΑΘΑΝΑΣΙΟΥ Α.Β.Ε.Ε.
Boil the penne according to the cooking times stated on the packet and keep the pasta warm.
Prepare the sauce while the pasta is boiling. Sauté the garlic in a pan over medium heat until caramelised, without burning it, and add the cherry tomatoes cut in half. Also add the red pepper and continue to sauté until soft. Add the vegetable stock and broccoli, and continue to boil until the broccoli is ready and the liquids have been absorbed. Throw the penne and goat cheese into the pan and mix so that the ingredients can blend.


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Houston's happy return
Fri 7 Nov 2014, 01:53 PM
As soon as Chris Houston signed with the Newcastle Knights in 2008, he knew it was a Club he never wanted to leave.
So the fact he's re-signed with the Knights for an additional two years, has the 29-year-old forward excited about the future and motivated to establish a stronger leadership role.
"It's something I always wanted from when I first came up here," he told Knights TV after his first pre-season training session on Thursday.
"I was confident with (Rick) Stoney coming on board that we'd be able to sort something out and I'm looking forward to playing under him for the next two years."
As one of the more experienced players among the squad, Houston's enthused about the Club's new recruits and up-and-coming players.
"We have a lot of young guys coming up from our successful youth competition and that's always great because there's plenty of energy in the young guys," he said.
"I'm definitely looking forward to helping these young guys and passing on a bit of knowledge I've learnt off Wayne (Bennett) and also what Stoney is teaching the guys as well.
"We have some new faces here at the Club this year, Tariq Sims is one of the most well known one from the Cowboys, and he'll be a great addition to our side.
"Jack Stockwell will be definitely keen to be pushing into that starting side.
"It will be good working with those guys and getting to know them a lot better."
There are also new faces off the field and Houston expects the wealth of experience and chemistry between head coach Rick Stone and assistant coaches Danny Buderus and Craig Sandercock to bring out the best in him and his teammates.
"I really enjoyed playing under Stoney and I think I played some of my best football when he was coach in 2009," he said.
"Danny Buderus has come on board with a more hands-on role this year with the team.
"His game experience is pretty much second to none.
"Craig Sandercock is a great coach too and those three are really working well together out there.
"It was a good mix with a bit of speed and a bit of conditioning then a lot of skill development which is going to be good for our team I think.
"I've learnt a fair bit today already in session one.
"It will be plenty of hard work no doubt, they always are. They don't really get too much easier the older you get."


Tags: would buy foldable smartphone? amazing smartphones anyone would love see under christmas tree would buy nokia with display carl zeiss camera optics?
Would you buy the 6-inch Galaxy S8 Plus or the 5.2-inch regular Galaxy S8?
We have heard of it many times earlier and once again, another report from Korea is indicating the same thing; a 6-inch or bigger model of the Samsung Galaxy S8. According to the latest report, both versions of the S8 will sport dual curved displays this time around, but the S8 Plus will have a 6-inch display instead of the 5.5-inch one we are used to seeing on the larger models of Samsung's S-series every year. The regular S8 will sport the 5.2-inch form factor just as its predecessors, albeit with a curved display.
The report also states that the S8 Plus, in spite of having a larger display than the Galaxy Note 7, will sport similar dimensions. This brings us to an almost established rumor about the Galaxy S8; the edge-to-edge bezel-less display. As you can imagine, the 90% or above screen to body ratio will allow the 6-inch S8 Plus to be just as manageable as the Note 7 was, by cutting out almost all bezel. Rumor also has it that the Galaxy S8 and S8 Plus will be launched at a higher price than its predecessors in April, at an exclusive event in New York.
Tags: would buy foldable smartphone? amazing smartphones anyone would love see under christmas tree would buy nokia with display carl zeiss camera optics? would buy 6-inch galaxy plus 5.2-inch regular s8?
Would You Buy This Galaxy S8 Clone?
If you want the real thing, you will have to wait till the end of April to get your hands on one, but China already has something cheaper for you. We are not exactly sure how much this would cost, but it should be under $100. That's the price you will have to pay if you want your friends to be jealous of you for having the world's most anticipated phone that isn't even out yet! Of course the jealousy will only be temporary as a closer look will reveal the handset to be the fake it is.
If all of that got you interested, here's an unboxing video of the S8 clone, courtesy of EverythingApplePro on YouTube. We also have multiple images for you from SamMobile. While they are definitely not the same device the actual internals might just be similar. Take a look and decide for yourself.
The best thing about this phone is that in spite of being a knock off, it actually looks pretty good with that curved dsiplay! We are sure that the Quad HD 5.2-inch display isn't really true, along with the rest of the features that you will find printed out on the back of the box. But even then, this doesn't look like a cheap clone at all. If this leak got you excited for the original S8, you will just have to wait for three weeks now, because that's when (March 29) Samsung will officially unveil the S8 and the S8+. In the meanwhile, take a look at this leaked video which almost certainly shows the real thing.
Tags: would buy foldable smartphone? amazing smartphones anyone would love see under christmas tree would buy nokia with display carl zeiss camera optics? would buy 6-inch galaxy plus 5.2-inch regular s8? would buy this galaxy clone?
Tags: would buy foldable smartphone? amazing smartphones anyone would love see under christmas tree would buy nokia with display carl zeiss camera optics? would buy 6-inch galaxy plus 5.2-inch regular s8? would buy this galaxy clone? would like see violet galaxy s8?
Would You Buy the Galaxy S8+ with 6GB RAM for $1,000+?
If you have been following the buzz circulating around the Galaxy S8 and the Galaxy S8+, then you probably already know that Samsung does indeed have a 6GB RAM variant of the larger Galaxy S8+ up its sleeves, just like the rumors had suggested before the news was confirmed by Samsung officially. However, many of us were disappointed when it was revealed that the 6GB RAM variant, which also has 128GB of internal storage, is going to be exclusive to South Korea for now.
Nevertheless, a new report out of Samsung's home nation is saying that this could change. The 6GB/128GB S8+ will likely make its way into at least a few other regions as well, if Samsung feels that enough customers in the area want the device. China is probably the most prominent example of a country which loves their smartphones with more memory and internal storage. Samsung's first and only 6GB RAM phone, the Galaxy C9 Pro was released there first. We will soon find out whether or not Samsung decides to bring it to any European countries or the US. However, the question remains; if it does indeed come to a store near you, would you be willing to shell out more than a thousand dollars for the extra storage and RAM?
Tags: would buy foldable smartphone? amazing smartphones anyone would love see under christmas tree would buy nokia with display carl zeiss camera optics? would buy 6-inch galaxy plus 5.2-inch regular s8? would buy this galaxy clone? would like see violet galaxy s8? would buy galaxy s8+ with 6gb ram 000+?
Would You Buy an 8K TV from Samsung for $15,000?
There is just something about large screen TV sets that the world loves and there's no denying it, but would you spend $15,000 on one? Well, that's the price you will have to pay if you want to buy the 85" Samsung Q900R QLED 8K TV that the company just unveiled. There will also be lower-priced options available at the 65", 72" and 75" categories, but the prices for the smaller TVs has not yet been confirmed by Samsung. Keep in mind that this is only the base price for the US, and if you live in India or any other country with exorbitant "luxury taxes" and import fees, you are probably looking at a much higher price tag for sure!
The Q900R will be capable of advanced HDR because the peak brightness level goes up as high as 4,000 nits (which is pretty insane)! The Samsung AI-powered software is all set to upscale the source images to 8K according to the announcement and that's where the real question pops in; what will you watch on your new super expensive 8K TV that will justify the price you just paid for it? 4K is not even a universal standard yet, so rest assured that aside from enthusiast grade content, you will likely have nothing to make use of those pixels. Given that 8K has four times as many pixels as 4K and 16 times the pixel count of full HD content, it would be fair to say that you will need an insanely fast internet connection, supported by ungodly amounts of data consumption allowance to stream 8K content, even if you find it anywhere!
Tags: would buy foldable smartphone? amazing smartphones anyone would love see under christmas tree would buy nokia with display carl zeiss camera optics? would buy 6-inch galaxy plus 5.2-inch regular s8? would buy this galaxy clone? would like see violet galaxy s8? would buy galaxy s8+ with 6gb ram 000+? latest galaxy note renders out but they different what would expect would buy samsung $15 000?
There have been speculations about a 4-inch smartphone manufactured by Apple for many months now. But, it doesn't have any official backing till now. As per some of the reported news, this might change in couple of weeks. The major thing under debate has been the name of this device. From the very beginning some reported it would be called as the iPhone 6c, others started referring it as iPhone 7c, and today a Chinese analyst claims that neither of the names will be used. It will be known as the iPhone 5e, where e stands for "enhanced".
As per a report from MyDrivers, FoxConn is currently working on the production of iPhone 5e and it'll on on sale sometime in March. This device is supposed to be priced at around $500, replacing the iPhone 5s as the new mid-range smartphone. The report also says that the smartphone will feature an A8 processor backed by 1GB of RAM and 16/64GB of storage. The iPhone 5e will have in-built support for Apple Pay and feature voice over LTE. The primary camera will have a 8 MP unit. The report directly denies other rumors that reported about phone's better specs, with an A9 chip, as available in the iPhone 6s series. When it comes to design, the iPhone 5e will look like Phone 5s, though some reports have pointed out that the phone might feature a curved "2.5D" display as found in the iPhone 6 and iPhone 6s. In China, the iPhone 5e would roughly cost CNY 3,288, which currently equals $500 or €459.
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Would Apple Release a Dual Sim iPhone?
Dual SIMs have pretty much become the standard among international Android phones, but just like the Micro-SD card, Apple has always shied away from the concept itself. According to a few new reports though, that is probably going to change this year because the new and upcoming iPhones may just have support for two SIMs.
This move could bring Apple closer to their Indian market and that is most likely the reason as to why the Cupertino giant might be thinking about dual SIMs in the first place. After all, India did overtake the US as the number two smartphone market in the world. Given that China is the biggest smartphone market in the world and the customers there also prefer dual SIM phones, the move actually makes even more sense.
When it comes to Apple, a lot of their exclusivity has been in offering "less for more," and ironic as it maybe, that is how they do business and a lot of people actually love them for it! They got rid of the headphone jack, never adopted the micro-SD card slot and there is no fingerprint scanner on their current flagship handset, but these omissions have done little to hamper their sales figures, not to mention they have actually managed to push their profit margin even higher on all their devices! It would be interesting to see if Apple really does adopt the dual SIM technology; something which everyone else had adopted years ago.
Tags: would buy foldable smartphone? amazing smartphones anyone would love see under christmas tree would buy nokia with display carl zeiss camera optics? would buy 6-inch galaxy plus 5.2-inch regular s8? would buy this galaxy clone? would like see violet galaxy s8? would buy galaxy s8+ with 6gb ram 000+? latest galaxy note renders out but they different what would expect would buy samsung $15 000? apple's new 4-inch smartphone would named iphone would like see iphone deep blue? would apple release dual sim iphone?
Tags: would buy foldable smartphone? amazing smartphones anyone would love see under christmas tree would buy nokia with display carl zeiss camera optics? would buy 6-inch galaxy plus 5.2-inch regular s8? would buy this galaxy clone? would like see violet galaxy s8? would buy galaxy s8+ with 6gb ram 000+? latest galaxy note renders out but they different what would expect would buy samsung $15 000? apple's new 4-inch smartphone would named iphone would like see iphone deep blue? would apple release dual sim iphone? how would like android oreo?
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Middle-Earth: Shadow of War Gets a Pretty CG Trailer, Xbox Play Anywhere Support
By Alex Chavers February 27, 2017
Thanks to Target we already knew another Middle-Earth game was on the way. Today, Warner Bros. makes it official with two and a half minutes of Middle-Earth cinematics. The August 22 release date was also confirmed. Here's our first official look at Shadow of War ahead of a March 8 gameplay reveal.
Cool, just make another Ring of Power to fight Sauron. Makes sense. Why did we have to watch Frodo and company go on a hiking trip? Just slap a new ring together and kick some ass. Obviously, the Middle-Earth video game franchise isn't canon. At least, I hope not for the sake of lore fans.
A new Ring of Power does open the possibilities for new gameplay mechanics that were teased in yesterday's leak. The trailer's description also teases an expanded Nemesis system and how it affects the game world.
"In Middle-earth: Shadow of War, players wield a new Ring of Power and confront the deadliest of enemies, including Sauron and his Nazgul, in a monumental battle for Middle-earth. The open-world RPG is brought to life through the expansion of the award-winning Nemesis System. The robust personalization from the first game is now applied to the entire world where the environments and characters are all shaped by player actions and decisions, creating a personal world unique to every gameplay experience."
Those are some big promises. They always sound good on paper, but often fail to materialize in ways we hope. Give me a more in-depth Nemesis system and better combat, and I'll be set. We should get a glimpse at how it's shaping up in next week's gameplay trailer.
Xbox Play Anywhere and Third-Party Games
Middle-Earth: Shadow of War will be a Xbox Play Anywhere title. That means you buy it once and play it on Windows 10 and Xbox One. Including third-party games might be news for some of you, but we just saw it happen with Resident Evil 7: Biohazard.
We've also seen it with smaller titles like Astroneer and Everspace. I say keep them coming. I doubt we'll ever see the likes of Call of Duty, but we can hope. Resident Evil 7 and Middle Earth: Shadow of War aren't small games. Sure, they aren't the biggest games of the year – but they're big enough.
Also, Middle-Earth isn't exclusive to Xbox, so it'll be coming to Steam too.
Update for Project Scorpio?
A press release from Microsoft also mentioned the game will be available for Project Scorpio following the console's launch this holiday season. That puts Scorpio's release firmly in the holiday season window (Oct-Dec, most likely November) and it hints at specific support for Project Scorpio (maybe an update to take advantage of the boost in power?).
The question is, will these updates be game specific or are we looking at an across the board boost in games? E3 is a little over three months away, and we'll learn much more about Xbox's plans with Scorpio there.
Are you looking forward to Shadow of War?


April is all about "green": from signs of spring sprouting up in our gardens to celebrations of Earth Day today. It's the perfect time to think about reducing our carbon footprints. And when it comes to technology (think of all that electronic equipment you and your business own), it's even more important to plan how you're going to dispose of these items properly, when the time comes.
There are things we all can do, especially in the workplace, when disposing of electronics.
1. Reincorporate. Whenever possible, reincorporate equipment that's in good shape with any newer equipment and set-ups. At Shanahan, we encourage clients to keep their good equipment, even when they have "new system" in mind. We take the time to create a thoughtful design that incorporates the older equipment with the new so that you won't even notice the difference! Bottom line? Keep what works (this will save you money as well).
2. Re-Gift. From desktops to laptops to cell phones to speakers to mixing consoles to you-name-it, there are many great organizations that welcome your equipment as a donation. For example, according to its website, the Cristina Foundation "is a nonprofit organization that matches donated computer equipment with needy schools and nonprofit organizations around the world." Cell Phones for Soldiers was started by a 13-year-old girl from Massachusetts who wanted to make give people's old cell phones for soldiers serving our country overseas. Some computer manufacturers offer buy-back programs, and don't forget to look into donation programs for printer cartridges.
3. Recycle. If you can't donate your electronics equipment, you'll need to make sure you properly dispose of these items (i.e. not in dumps or landfills). The EPA website lists numerous resources for recycling programs on its website, including links on finding a local program in your city or town. The Electronic Industries Alliance (EIA) hosts a site called E-cycling Central, which allows you to see electronics recycling resources for all 50 states.
4. Realize that we all must do our part--at home and at work. At Shanahan Sound, we reincorporate equipment whenever possible, we donate when that makes sense, and everyone on our wonderful staff makes every effort to recycle.


In my experience, Kmart's prices are higher than the norm, but doubled coupons usually allow for FREE or inexpensive toiletries and cleaning products!
If I were going to shop the Doubles, I'd take the $2 All laundry coupons with me, as well as any special coupons that I may have received directly from a manufacturer (usually these are higher in value). P&G coupons are often higher in value, and I'd definitely look at the printable coupons at Coupons.com and Red Plum.com to see if there are any that might be more beneficial to spend at Kmart this week.
Keep in mind that the Kmarts that are doubling this week are the full grocery store Kmarts- so don't overlook spending your grocery coupons!
Lastly, Kmart now has a 'loyalty card' of sorts. You must register before you shop. You will have two options for redeeming your doubled coupons (15/day) before your perminant arrives in the mail: 1. print out a paper copy of your loyalty card (this is what I have done in the past) or 2. give the cashier your pone number at the register! ***Savvy Spending has the scoop on this card as well!
Best of luck! I'd LOVE to hear about the deals that YOU'VE scored!
Thank you for the post Laura. What store do you shop at? I'm trying to find the closest one to Brighton, MI.
Well, if I were to join in this weeks 'festivities' (which I probably won't), the Madison Heights location is the closest to me!


Home / Freebies / Free Stuff Online / Free Samples / FREE Breathe Right Sample to 25,000!
HURRY and snag a FREE Breathe Right Sample for the first 25,000 who sign up! When you "like" Breathe Right on Facebook you will be able to request your sample! You will get 2 sample packs of Breathe Right Advanced!
Hurry and snag this now, this will go fast!


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This colorful Talit also known as Bnai-Or Talit imitates Josephs multi-colored coat. Comes in 13 different colors or in 13 different shades of blue to astonish and amaze all. Also available a perfectly matching bag and Kipa with this Talit. Many different sizes available to fit all ages for the most comfortable feeling when praying with this Talit.


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SPEAKERS - TEXTS & BIO
APPEAL FOR PEACE
Cyprus: the history
Toward the Meeting in Cyprus
THE PLACES OF THE MEETING
TEXTS AND SPEAKERS
TV WEB SAT BROADCAST
AVAILABLE VIDEOS
MESSAGE FROM H.H. BENEDICT XVI
Krakow 2009
NEWS FROM THE MEETING
METHOD OF ACCREDITATION
HOW TO FOLLOW LIVE THE EVENT
ALL VIDEOS OF THE EVENT
PHOTO & VIDEO GALLERY MONACO 2011
Pictures from Munic
Go to Home Page in english
VIDEO & PHOTOGALLERY
ROME 2013 Religions and Cultures: Dialgue and Peac
WEB & TV
Linked Events
PLACES OF THE MEETING
Antwerp 2014 Peace is the future
ANTWERPEN-2014 HOME PAGE
Tirana 2015 - Peace is Always Possible
TIRANA-2015 HOME PAGE
BARI 2015: Christians in the Middle East: what future?
Home Bari 2015
Partecipants
#SaveAleppo
HOME Firenze 2015
RELATORI - TESTI & BIO
Video e FotoGallery
Fotogallery - #civilitzationsindialogue. Firenze 2015
Assisi 2016 - Thirst for Peace
Assisi 2016 - Home page
Münster 2017 - Paths of Peace
HOME PAGE MÜNSTER 2017
Aspettando Strade di Pace - arrivi e backstage
Streaming programme
Events in streaming
ROME 2020 > ARCHIVE > MÜNSTER 2017 > FERGUSON CHRIS
Ferguson Chris
World Communion of Reformed Churches, Canada
The Reverend Chris Ferguson is the second general secretary of the World Communion of Reformed Churches (WCRC). He was installed into the office in May 2014 and took up the position in August.Chris Ferguson
Ferguson is an ordained minister in the United Church of Canada (UCC), obtaining his master of divinity degree from the Vancouver School of Theology in 1978.
From 2011-2014 Ferguson served in Colombia with the Programme for Ecumenical Accompaniment in Colombia (PEAC) as international ecumenical advisor to the programme, sent as global mission personnel of the UCC in conjunction with the Latin American Council of Churches–Colombia and the Presbyterian Church in Colombia. PEAC accompanies communities in Colombia that are victims of violence and engages in global ecumenical advocacy for a just peace.
Previous to this Ferguson worked for the World Council of Churches (WCC) in two different capacities, appointed by the UCC as seconded global mission personnel. From 2004 to 2006 he was the WCC's representative in Jerusalem, making connections between local and global organizations to promote understanding, reconciliation and peace in the region. From 2006 to 2010 Ferguson represented the WCC at the United Nations headquarters in New York City.Jerry Chris Najla
From 2002 to 2004 he was the executive minister of the Justice, Global and Ecumenical Relations Unit and ecumenical officer of the UCC. Previous to that he was the general secretary of the Division of World Outreach for three years and from 1991 until 1999 was the area secretary for the Caribbean and Latin America. In these roles he provided leadership in the UCC's ecumenical, mission, justice, human rights, theological education, development and partnership work in Latin America, the Caribbean and globally.
Within Canada Ferguson participated actively in several national ecumenical justice and human rights coalitions and was a member of the founding board of directors of Kairos Canada, as well as serving on the board of the Canadian Council of Churches. He also helped found local and national refugee rights groups.
From 1987 to 1991 he was appointed as UCC global mission personnel to work with the Latin American Biblical Seminary in San José, Costa Rica, where he was professor of theology and ministry, teaching and leading workshops throughout Central America in a context of war and civil conflict. He also was a visiting researcher in human rights and the church at the Ecumenical Research Department.Chris Taiwanese
Previous to his work in Central America, Ferguson was the Presbyterian-United Church chaplain and director of chaplaincy services at McGill University in Montréal, Quebec, for seven years. While at McGill he was an adjunct professor at the United Theological College and the Montréal Institute for Ministry where he taught courses in ministry, counselling and church and society. Prior to the years in Montréal he served as minister/rector of a joint United Church-Anglican parish in Northern Quebec and as a college teacher, counsellor and community worker in British Colombia where he founded a youth centre and a crisis intervention centre.
Over the last three decades Ferguson has been an active participant and leader in various WCC committees and commissions in Latin America and globally. As a member of the Heads of Agencies Network he helped initiate the first WCC funding roundtable. He has taken an active lead in global ecumenical advocacy and public witness for peace, justice, ecological justice and human rights.Africa Accra Consultation
He was on the World Alliance of Reformed Churches (a predecessor organization of the WCRC) executive committee for a period preceding the 2004 General Council in Accra, Ghana, participated in the "Covenanting for Justice Process" and played an active role on the drafting committee for the Accra Confession.
Ferguson is married to Susan, a professional social worker who has served as UCC global mission personnel in Colombia, Israel/Palestine and Costa Rica. Susan coordinated the UCC national refugee work and was executive director of an ecumenical refugee resettlement agency in Toronto. Susan and Chris have two adult children.
Ferguson is a graduate of Antioch College/West. Besides his master degree from the Vancouver School of Theology, his post-graduate theological studies were at the University of Montréal Faculty of Theology in the pastoral studies department. He also has advanced training and supervised practice in pastoral counselling and marriage and family therapy.
Source: World Communion of Reformed Churches
September 12 2017 09:00 | Petrikirche
PANEL 24: 500 Years After the Reformation: the Word of God and the Life of Christians
• Speech of Chris Ferguson
September 20 2016 08:30 | Monastero di Sant'Andrea
PANEL 23: Prayer at the Root of Peace
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UCI announces 5th doping case linked to Astana
AIGLE, Switzerland (AP) — The International Cycling Union has provisionally suspended Kazakh rider Artur Fedosseyev in the fifth doping case linked to the Astana team.
The UCI says Fedosseyev tested positive for an anabolic steroid on Aug. 16 when he was competing for Astana's development team at the Tour de l'Ain. The 20-year-old rider placed 15th.
The case was announced Tuesday while the UCI license commission reviews Astana's application to retain top-tier World Tour status next season.
The Kazakh government-backed team's star rider is Tour de France winner Vincenzo Nibali of Italy.
In recent weeks, Nibali's teammates Maxim and Valentin Iglinskiy have tested positive for EPO, and Ilya Davidenok tested positive for steroids.
Last week, another Astana development rider, Victor Okishev, was provisionally suspended after testing positive for an anabolic steroid.


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0501-17 New York Times Crossword Answers 1 May 17, Monday
CROSSWORD CONSTRUCTOR: Ed Sessa
THEME: -arie Antoinette
Today's crossword centers on Marie Antoinette, although it's OFF WITH HER HEAD. The queen was deemed to have committed a CAPITAL OFFENSE, so the capital M in "MARIE" has been lopped off:
41A. Famous queen, depicted literally : -ARIE ANTOINETTE
20A. Words attributed to 41-Across : LET THEM EAT CAKE
24A. Apt cry for 41-Across : OFF WITH HER HEAD!
47A. Something committed by 41-Across … or by this puzzle's creator? : CAPITAL OFFENSE
BILL BUTLER'S COMPLETION TIME: 5m 24s
1. Bouts, as of crying : JAGS
The word "jag" is used to describe periods of unrestrained activity, particularly involving alcohol, and has been in use since the 1800s.
5. ___ Le Pew (cartoon skunk) : PEPE
Pepé Le Pew is a very likeable cartoon character from the Looney Tunes and Merrie Melodies series. Pepé is a French skunk, first introduced way back in 1945. He is always thinking of "l'amour" and chases the lady skunks, or a black cat with a white stripe accidentally painted down her back.
9. Fizzy headache remedy, informally : BROMO
Bromo-Seltzer is an antacid that was first produced back in 1888, and is still available today, although it's hard to find. The Bromo-Seltzer headquarters were in downtown Baltimore in a building that features a magnificent clock tower that was patterned on the Palazzo Vecchio in Florence. The tower is a famous landmark in the city. The four clock faces display the letters B-R-O-M-O S-E-L-T-Z-E-R beside the much smaller Roman numerals that tell the time.
20. Words attributed to 41-Across : LET THEM EAT CAKE
There is no evidence that Marie Antoinette ever uttered the words "Let them eat cake". The phrase was attributed to "a great princess" though, by the philosopher Jean-Jacques Rousseau. Rousseau wrote in his autobiography:
Finally I recalled the stopgap solution of a great princess who was told that the peasants had no bread, and who responded: "Let them eat brioche (cake)."
22. Wyatt of the Old West : EARP
Wyatt Earp is famous as one of the participants in the gunfight at the O.K. Corral. Earp was a city policeman in Wichita, Kansas and also in Dodge City, Kansas. Earp was also deputy sheriff in Tombstone, Arizona where the O.K. Corral gunfight took place. Years later, Earp joined the Alaska Gold Rush and with a partner built and operated the Dexter Saloon in Nome.
31. Moving day rental : U-HAUL
The U-Haul company was started by married couple Leonard Shoen and Anna Mary Carty in Ridgefield, Washington in 1945. The Shoens used $5,000 of seed money to build trailers in their garage, and then cleverly recruited gas station owners as franchisees with whom they would split the rental revenue. There are now about 15,000 U-Haul dealers across the country.
34. Cosmologist Sagan : CARL
Carl Sagan was a brilliant astrophysicist and a great communicator. Sagan was famous for presenting obscure concepts about the cosmos in such a way that we mere mortals could appreciate. He also wrote the novel "Contact" which was adapted into a fascinating 1997 film of the same name starring Jodie Foster.
38. Fleur-de-___ : LIS
"Lys" (also "lis") is the French word for "lily", as in "fleur-de-lys", the heraldic symbol often associated with the French monarchy.
40. Dresses in Delhi : SARIS
New Delhi is the capital city of India. New Delhi resides within the National Capital Territory of Delhi (otherwise known as the metropolis of Delhi). New Delhi and Delhi, therefore, are two different things.
41. Famous queen, depicted literally : -ARIE ANTOINETTE
Marie Antoinette was the wife of Louis XVI, the last king of France. Marie Antoinette was the fifteenth of sixteen children born to the Empress Maria Theresa of Austria. The marriage to Louis, her second cousin once removed, was arranged while the two were very young. The prospective bride was "handed over" to the French at a border crossing in 1770 and two weeks later she was married to the future king. Marie Antoinette was just 14 years of age at the time, and Louis only a year her senior. Both Louis and Marie Antoinette were doomed to lose their heads courtesy of the guillotine during the French Revolution.
45. Early Beatle ___ Sutcliffe : STU
Stu Sutcliffe was one of the original four members of The Silver Beatles (as The Beatles were known in their early days), along with John Lennon, Paul McCartney and George Harrison. Sutcliffe apparently came up with name "Beatles" along with John Lennon, as a homage to their hero Buddy Holly who was backed by the "Crickets". By all reports, Sutcliffe wasn't a very talented musician and was more interested in painting. He went with the group to Hamburg, more than once, but he eventually left the Beatles and went back to art school, actually studying for a while at the Hamburg College of Art. In 1962 in Hamburg, Sutcliffe collapsed with blinding headaches. He died in the ambulance on the way to hospital, his death attributed to cerebral paralysis.
57. Competitor of Wisk : TIDE
Tide is a laundry detergent that has been made by Procter & Gamble since 1946. Back then, Tide was marketed as "America's Washday Favorite".
5. Czar called "the Great" : PETER I
Peter the Great was perhaps the most successful of the Romanov tsars, and was famous for modernizing Russia and expanding the country's sphere of influence, creating the Russian Empire. He ruled from 1682 until his death in 1725.
8. Grammy-winning James : ETTA
Etta James was best known for her beautiful rendition of the song "At Last". Sadly, as she disclosed in her autobiography, James lived a life that was ravaged by drug addiction leading to numerous legal and health problems. Ms. James passed away in January 2012 having suffered from leukemia.
12. Bit of viral web content : MEME
A "meme" (short for "mineme") is a cultural practice or idea that is passed on verbally or by repetition from one person to another. The term lends itself very well to the online world where links, emails, files etc. are so easily propagated.
24. Atheist Madalyn Murray ___ : O'HAIR
Madalyn Murray O'Hair was the president of the organization American Atheists from 1963 to 1986. Murray was the plaintiff in the lawsuit Murray v. Curlett that in 1963 led to the Supreme Court ruling that ended Bible-reading in American schools. Murray came to a sorry end, as she was kidnapped in 1995 and brutally murdered along with her son and granddaughter. The crime was committed by a co-worker at American Atheists.
25. Persian tongue : FARSI
"Farsi" is one of the local names for Persian, an Iranian language.
28. Fellow film critic of Siskel : EBERT
Roger Ebert co-hosted a succession of film review television programs for over 23 years, most famously with Gene Siskel until Siskel passed away in 1999. Siskel and Ebert famously gave their thumbs up or thumbs down to the movies they reviewed.
30. Belles of the ball : DEBS
"Deb" is short for "debutante", which translates from French as "female beginner".
31. The Bruins of the Pac-12 : UCLA
The UCLA Bruins mascots are Joe and Josephine Bruin, characters that have evolved over the years. There used to be "mean" Bruin mascots but they weren't very popular with the fans, so now there are only "happy" Bruin mascots at the games.
35. Krispy Kreme product : DONUT
The Krispy Kreme chain of doughnut stores was founded in 1937 in Winston-Salem, North Carolina. The company introduced the Whole Wheat Glazed doughnut in 2007, great for folks looking to eat a healthy diet, I am sure …
36. Grain used in making Alpha-Bits : OAT
Alpha-Bits is a Post breakfast cereal that was introduced in 1958, and taken off the shelves in 2006. The cereal was relaunched in 2008 with "0% Sugar!" tagline, but I guess that didn't sell too well. The original Alpha-Bits formulation was reintroduced in 2008. Sugar, sugar, sugar …
40. Feudal workers : SERFS
A serf was a member of the lowest feudal class, someone attached to land owned by a lord. "Serf" comes from the Latin "servus", meaning "slave".
42. ___ de corps (fellowship) : ESPRIT
"Esprit de corps" is the morale of a group, best translated from French perhaps as "team spirit".
43. Tristan's beloved : ISOLDE
According to Arthurian legend, Iseult (also "Isolde") was the adulterous lover of Sir Tristan, one of the Knights of the Round Table. Iseult was an Irish Princess who fell in love with Tristan who had been sent to win Iseult's hand in marriage for King Mark of Cornwall. The tale was used as the basis for Richard Wagner's celebrated opera "Tristan und Isolde".
47. Brilliant move : COUP
A coup d'état (often just "coup") is the sudden overthrow of a government, and comes from the French for "stroke of state". The Swiss-German word "putsch" is sometimes used instead of "coup", with "Putsch" translating literally as "sudden blow".
49. "The Thin Man" canine : ASTA
Asta is the wonderful little dog in the superb "The Thin Man" series of films starring William Powell and Myrna Loy (as Nick and Nora Charles). In the original story by Dashiell Hammett, Asta was a female Schnauzer, but on screen Asta was played by a wire-haired fox terrier called "Skippy". Skippy was also the dog in "Bringing up Baby" with Cary Grant and Katharine Hepburn, the one who kept stealing the dinosaur bone. Skippy retired in 1939, so Asta was played by other dogs in the remainder of "The Thin Man" films.
50. Of the flock : LAIC
Anything described as laic (or "laical, lay") is related to the laity, those members of the church who are not clergy. The term "laic" ultimately comes from the Greek "laikos" meaning "of the people".
53. Jane Austen heroine : EMMA
Jane Austen's novel "Emma" is the tale of Emma Woodhouse and the wonderful George Knightley. At the end of the story, Emma marries Knightley and her young friend Harriet marries Robert Martin, who had been trying to get Harriet's attention practically from page one of the novel!
54. Roast hosts, for short : MCS
The term "emcee" comes from "MC", an initialism standing for Master or Mistress of Ceremonies.
14. Bra size smaller than a "B" : A-CUP
15. Highway sign next to an off-ramp : EXIT
16. Extend, as a subscription : RENEW
17. Not giving up on an argument, say : INSISTENT
19. Throat-clearing sounds : AHEMS
23. Hi-___ monitor : RES
24. Apt cry for 41-Across : OFF WITH HER HEAD!
32. Big laughs, slangily : YUKS
33. Symbol of busyness : BEE
35. Ninnies : DOPES
37. Parsley, sage, rosemary or thyme : HERB
39. Money that needs to be repaid : LOAN
46. Put into piles, say : SORT
47. Something committed by 41-Across … or by this puzzle's creator? : CAPITAL OFFENSE
54. Attend a funeral, say : MOURN
55. Merchandise location : SALESROOM
56. Keepsake in a cabinet, perhaps : CURIO
58. First among men : ADAM
59. Minor fights : SPATS
60. Cards sometimes hidden up sleeves : ACES
61. Blood components : SERA
1. Monopoly space with the words "Just Visiting" : JAIL
2. Pimples : ACNE
3. Wind that might blow one's hat off : GUST
4. Malicious : SPITEFUL
6. Not taxable, e.g. : EXEMPT
7. Wood for many a mountain cabin : PINE
9. Pick-me-up drinks : BRACERS
10. It's nothing new : REHASH
11. Short race spec : ONE-K
13. Cries of pain : OWS
18. Article thrown over the shoulders : SHAWL
21. Long, hard journeys : TREKS
26. Prefix relating to sleep : HYPNO-
27. Shade of color : HUE
29. Eagle's home : AERIE
37. North Carolina's Cape ___ : HATTERAS
39. Many Americans whose names end in -ez : LATINOS
44. Certain bank policy for A.T.M. withdrawals and wire transfers : NO FEES
48. Ambient quality : AURA
51. Point where lines meet : NODE
52. Fly high : SOAR
Posted on May 1, 2017 October 3, 2017 Categories Ed Sessa13 Comments on 0501-17 New York Times Crossword Answers 1 May 17, Monday


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Home News Pentagon Chief Asks for Navy Secretary's Resignation Over Private Proposal in Navy...
Pentagon Chief Asks for Navy Secretary's Resignation Over Private Proposal in Navy SEAL's Case
Elena SkladUSA
WASHINGTON (The Washington Post) –
Sunday, November 24, 2019 at 6:08 pm | כ"ו חשון תש"פ
Navy Secretary Richard Spencer in July, 2018. (AP Photo/Eugene Hoshiko/File)
Defense Secretary Mark Esper asked for the resignation of Navy Secretary Richard Spencer on Sunday after losing confidence in him over his handling of the case of a Navy SEAL accused of war crimes in Iraq, the Pentagon said.
Spencer's resignation came in the wake of the controversial case of Chief Petty Officer Edward Gallagher, a Navy SEAL who was accused of war crimes on a 2017 deployment. He was acquitted of murder but convicted in July of posing with the corpse of a captive.
Esper asked for Spencer's resignation after learning that he had privately proposed to White House officials that if they did not interfere with proceedings against Gallagher, then Spencer would ensure that Gallagher was able to retire as a Navy SEAL, with his Trident insignia.
Spencer's private proposal to the White House – which he did not share with Esper over the course of several conversations about the matter – contradicted his public position on the Gallagher case, chief Pentagon spokesman Jonathan Hoffman said in a statement.
Esper said in the statement that he was "deeply troubled by this conduct."
"Unfortunately, as a result I have determined that Secretary Spencer no longer has my confidence to continue in his position," Esper said. "I wish Richard well."
Spencer's spokeswoman did not immediately respond to a request for comment.
Esper and Army Gen. Mark Milley, the chairman of the Joint Chiefs of Staff, learned of Spencer's private offer to the White House when they spoke with President Donald Trump on Friday, Hoffman said.
Spencer's proposal to the White House came after Trump intervened in the cases of Gallagher and two soldiers on Nov. 15. Countering Pentagon recommendations, the president issued pardons to Army Maj. Mathew Golsteyn, who faced a murder trial next year, and former 1st Lt. Clint Lorance, who was convicted in 2013 in the murder of two unarmed men in Afghanistan.
Trump reinstated Gallagher's rank after the SEAL was demoted as punishment for posing for the photograph with the corpse. As a result of the actions over the past few days, Hoffman said, Esper has decided to let Gallagher keep it.
Spencer made his private pitch to the White House in conversations before a Thursday tweet by Trump, in which the president publicly pushed back against the Navy launching a review that could have stripped Gallagher of his Navy SEAL status.
"The Navy will NOT be taking away Warfighter and Navy Seal Eddie Gallagher's Trident Pin," Trump wrote. "This case was handled very badly from the beginning. Get back to business!"
Hoffman said Esper has suggested to Trump that Kenneth Braithwaite, a retired Navy rear admiral who is the U.S. ambassador to Norway, be considered as the next Navy secretary.
Secretarys
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Bruno Mars stars in the latest episode of Carpool Karaoke.
Off the heels of his album release for 24K Magic, Bruno Mars is set to join James Corden on Carpool Karaoke. In celebration of the new episode, Bruno previewed an image of him and the Late Late Show's host on Instagram Saturday night (Nov. 19).
"When an unstoppable force meets an immovable object @j_corden Thanks for the lift," Bruno captioned a selfie he took with Corden in the car. Like other guests that have graced the passenger seat of Corden's ride, Bruno will be featured helping the talk-show host get to work, while singing along to his greatest hits.
Bruno's announcement comes only days after Madonna revealed that she would be carpooling with Corden in the upcoming weeks. Past guests have included Jennifer Hudson, J.Lo, Adele, First Lady Michelle Obama, and Missy Elliott.
Bruno Mars released 24K Magic on Nov. 18. The album includes popular songs "24K Magic," "Versace on the Floor," and features appearances from Babyface and audio from Halle Berry. The singer also revealed that he would be going on tour in support of the record.
A specific air time for Bruno Mars' Carpool Karaoke episode has not been announced, but we're pretty sure it's going to be dope.


Lead. — 24. Goetze of Wurzburg. Die Bleivergiftung. — 25. Gombault and Charcot.
exhibitions and publications devoted to the subject of Ars Medica, to fruition.
fering with its progress was concerned, was without avail.
Swift, J. B. A case of Alexander's operation, 421, 427.


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America Three Fifty Six
There are some photographs that seem almost like they float above time. This is a great example.
Published by Ben Branch - May 20th 2012
90th Anniversary Schott Perfecto Jacket – The Jacket That Started It All
The 90th Anniversary Schott Perfecto Jacket was developed to celebrate 90 years since Irving Schott designed America's most iconic leather motorcycle jacket – this anniversary edition was created by the 3rd and 4th generations of the Schott family. The Perfecto The original Perfecto Jacket was named for Irving Schott's favorite cigar type, the jacket was…
Ferrari 512 BB "Competizione" – A 475 HP Flat-12 Track Weapon
The Ferrari 512 BB "Competizione" was the competition version of the standard 512 BB – Ferrari offered a "Le Mans" kit to upgrade standard cars after a flood of requests from serious customers. The development of the racing version was started by Ferrari's Customer Assistance department in 1978. Once completed the kit would offer significant…
The 1966 General Motors Three-Wheeler Designed By Peter Maier
Peter Maier At the age of 20, Maier was one of the youngest designers ever hired by General Motors Corporation. After seeing Maier's work at the 1964 New York World's Fair, renowned automotive designer and VP of GM, Bill Mitchell, offered Maier a position for their 3-month Summer Program. Halfway through the program Maier was offered…
2000 Ford SVT Mustang Cobra R – 385 BHP – 0-60 MPH in 4.4 Seconds
When the 2000 Ford SVT Mustang Cobra R debuted it was both the fastest and most technologically advanced production Mustang ever built. The 2000 Cobra R had independent front and rear suspension, race ready brakes, a Tremec T-56 6-speed gearbox, and a DOHC 32-valve V8 fitted with alloy heads that would eventually find their way…
The California Star – A Ford Model T-Inspired Custom Roadster From The 1980s
The California Star has always been a controversial car, it was built in the early 1980s as a modern day interpretation of the Ford Model T Roadster using a full space frame chassis, competition grade suspension and Wilwood brake calipers, a mid-mounted turbocharged engine, Porsche 911 rack and pinion steering, and a hand-formed aluminum body….
This Is The Sabra Sport GT – The First Israeli Sportscar
The Sabra Sport GT built holds the unique distinction of being the only production Israeli sports car ever made, it was built by Autocars Co. Ltd – Israel's first domestic car manufacturer.
Aston Martin DB4 Sports Racer
Bomber Boys


Discover this gem of a rest stop, located on Route 138 right on the banks of Lake Saint-Pierre. Stop to admire the view and rest a while, with picnic tables and restrooms available. Be sure to admire the breathtaking sunsets if you're in the area!
This well-kept secret is like something you'd drive miles and miles to the seashore to discover—except it's located right here at home! The little lakeside shack serves seafood favourites, from "lobster roll"style smoked trout sandwiches to gravlax, breaded shrimp, calamari, fish chowder, gelato, and a whole lot more. Tantalize all your senses with a breathtaking view and authentic food. The Sea Shack Au Bord du Lac is a summer must!


Once upon a time in a faraway land, there lived a Chinese wise man and his disciple. One day in their travels, they saw a hut in the distance. As they approached they realized that it was occupied in spite of its extremely poor appearance.
I just got a meaningful sharing in one of my Wats App group. I found it very interesting and decided to share it in this platform. One of the things I like most is that this story talks about to embrace the new opportunities and through out the habit of dependency. Dependency is the Biggest Obstacle to Growth. Perhaps, some of you may already know this story, but there's always somebody who never heard it. In any case, it's a lesson worth remembering.
The wise man, an elderly Chinese man, took a deep breath and repeated the order: "Go ahead. Push the cow off the cliff." The student was resigned to obey his master. He returned to the hut and quietly led the animal to the edge of the cliff and pushed. The cow fell down the cliff and died.
Three good-looking teenagers and their parents were celebrating their first million dollars.
The heart of the disciple froze. What could have happened to the family? Without a doubt, they must have been starving to death and forced to sell their land and leave. He approached the house and asked a man that was passing by about the whereabouts of the family that had lived there several years before.
"You're looking at it," said the man, pointing to the people gathered around the barbecue. Unable to believe what he was hearing, the disciple walked through the gate and took a few steps closer to the pool where he recognized the man from several years before, only now he was strong and confident, the woman was happy, and the children were now nice-looking teenagers.
It turns out that once they stopped being reliant on the cow, they found other skills and gifts that were lying dormant. When the cow died it forced them out of their comfort zones and made them actually use their potential.
Sometimes our dependency on something is the biggest obstacle to our growth. Once you free yourself of the thought of dependency — then your life will really change.
Don't set targets based on where you were last year, set targets based on where you want to go.
If there is a cow (a person, idea, mental block or mind set) in your life that is keeping you miserable, – throw the cow off the cliff and embrace new opportunities.
Make the most, what you have, says Lord Ganesha.
If you have any inspirational article or success story of any students or entrepreneur, please share with us here.
How man can grow with increasing cows if he can grew without cow, he can grow with the help of cow. Question is mindset to grow. What we do by force is gives 100% result but instead of forcing, we can educate people to comeout.
Any method to educate people to comeout?
at first, I thought "why he wants to kill the cow? that so mean. ….. but than I read this full story and get your point…… Through the cow of the cliff and Embrace the opportunity.
Cow means dependency on a person, or idea or style…mind set mind block, comfort zone, etc…….
This is a great story. People tend to stick to a habit or routine having or doing only the bare minimum. Sometime we must reach out of our comfort zone and try new things that are good for us.


text:
Rheological properties of fermented milk produced by a single exopolysaccharide producing Streptococcus thermophilus strain in the presence of added calcium and sucrose
Purwandari, Umi and Vasiljevic, Todor (2009) Rheological properties of fermented milk produced by a single exopolysaccharide producing Streptococcus thermophilus strain in the presence of added calcium and sucrose. International Journal of Dairy Technology, 62 (3). pp. 411-421. ISSN 1364-727X (print), 1471-0307 (online)
The effect of calcium supplementation, presence of exopolysaccharides (EPS) and sucrose on the texture of low-fat fermented milk was examined. Reconstituted skim milk (14%) was acidified by 2% glucono-δ-lactone (GDL) or a single strain, EPS-producing Streptococcus thermophilus, and incubated at 42°C. The levels of calcium and sucrose addition ranged from 0 to 9 mm and 0 to 45 mm respectively. Compared with acidified milk (GDL), fermented milk containing EPS showed different rheological properties in the presence of added calcium and sucrose. Calcium addition reduced gel characteristics: G′, K, and the second maximum of derivative of shear rate-shear stress; while sucrose addition also increased them but subsequently reduced syneresis. Added calcium or sucrose increased gel hysteresis and reduced apparent yield stress.
Dimensions Badge
URI https://vuir.vu.edu.au/id/eprint/4537
DOI https://doi.org/10.1111/j.1471-0307.2009.00501.x
Official URL http://onlinelibrary.wiley.com/doi/10.1111/j.1471-...
Subjects Historical > Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
Historical > FOR Classification > 0908 Food Sciences
Historical > SEO Classification > 8602 Dairy Products
Keywords ResPubID17307, calcium, exopolysaccharide, low-fat yoghurt, rheology, sucrose
Citations in Scopus 10 - View on Scopus


text:
Crocynia is een geslacht van korstmossen behorend tot de familie Ramalinaceae.

Soorten
Volgens Index Fungorum bevat het geslacht vijf soorten (peildatum januari 2023):

Ramalinaceae
Schimmelgeslacht


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Top40's Most Searched for on 05/04/2019
2k Views by top40 May 3, 2019, 7:35 pm inInstagram Daily
Google "Daily Trends" sample taken on 05/03/2019 @ 6:30pm EST from Untied States
500K+ searches
Ted Bundy Who? yes I am old and wise and have heard of him. A mass murderer who lured girls while travelling around the country then killed them and got away with it for almost 15 years It would be a little bit harder to get away with that these days with all the cell phones and cameras everywhere. But there are still more unsolved murders than solved ones. So this particular paid for search generated probably by netflix or Mr.Efron himself and all for a semi Biographical Docudrama which is called "Extremely Wicked, Shockingly Evil and Vile" and distributed through Netflix and I suppose they helped produce it about who else Ted Bundy Needless to say we feel this #1 is a paid for search and definitely well worth the payment the Paid for Searches Not the movie!
Extremely Wicked, Shockingly Evil and Vile Trailer #2 (2019) | Movieclips Trailers
Check out the official Extremely Wicked, Shockingly Evil and Vile trailer starring Zac Efron! Let us know what you think in the comments below. ► Buy Tickets to Extremely Wicked, Shockingly Evil and Vile: https://www.fandango.com/extremely-wicked-shockingly-evil-and-vile-216981/movie-overview?cmp=MCYT_YouTube_Desc Want to be notified of all the latest movie trailers?
Colorado Avalanche in a game last night that would have been thursday 05/03 the avalanche an NHL hockey team playing against colorado in a 3-0 victory to even the series 2-2. It marked the first Colorado postseason shutout since 2010. Now we feel since the search is with in a 24 hour period and the game was available online this could be a legit #2 in our Most Searched for list. SO we will leave it at that and remind everyone we dont like many sports and really can hardly beleive that many people would care to search for Colorado Avalanche. So we will add there could have been some paid searches. But for any fans here are the highlights and some pics…..
NHL Stanley Cup Playoffs 2019: Sharks vs. Avalanche | Game 4 Highlights | NBC Sports
Philipp Grubauer records his first playoff shutout and Nathan MacKinnon extends his point streak to eight games as Colorado evens its series against San Jose, 2-2. #NBCSports #NHL #StanleyCupPlayoffs #Avalanche #Sharks " Subscribe to NBC Sports: https://www.youtube.com/nbcsports " Watch Live Sports on NBCSports.com: http://www.nbcsports.com/live " Get more NHL news on NBC Sports: https://www.nbcsports.com/nhl/stanley…
Sonic movie
Sonic movie definitely a paid for search no one even cares about Sonic the hedgehog or the restaurant. Coming in at #3 anyway the director says they will fix Sonic's appearance before it comes out. Okay well I liked it the way it was but anything to stir up some good old controversy hence more publicity. Especially if you cant think of anything else to talk about but the poor little baby's frighteningly horrific appearance. LOL
Sonic The Hedgehog (2019) – Official Trailer – Paramount Pictures
He's a whole new speed of hero. Watch the new trailer for Sonic The Hedgehog, in theatres this November. #SonicMovie SONIC THE HEDGEHOG is a live-action adventure comedy based on the global blockbuster videogame franchise from Sega that centers on the infamously brash bright blue hedgehog.
Caitlin Fink
50K+ searches
Caitlin Fink Who? never heard of her till today but she is a student former student IDK of some high school in california and the an article about her being in the Porn industry was published in the Bear Creek High School paper, The Bruin Voice, on Friday. Well its apparently at the heart of another debate about censorship because the school wanted to see the article first. Needless to say we are all for people doing whatever they want whenever they want as long as it doesnt infringe on anyone elses person or rights as we know them. So Cheers to Caitlin Fink.
California school newspaper publishes story on porn worker after free speech battle
California school newspaper publishes story on porn worker after free speech battle Subscribe us to read more: https://www.youtube.com/channel/UC77RVLoYD4veiFQR7q2Kstg?sub_confirmation=1 "I'm 18, what I'm doing is legal, and I don't see why everyone is making such a big deal out of it," Caitlin Fink said. Bruin Voice news editor Bailey Kirkeby wrote the article.
Shawn Mendes yes we have heard of him but cant believe anyone would care about what he said in a radio broadcast. even though he basically said he would kick Justin Beibers Ass. Now I couldn't honestly care less. So IMHO this is a paid for search. First off no one listens to that radio show, The Elvis Duran Show earlier this morning. The article wasn't even published till 3:20 today but according to our Google trends is coming in at #5. So how is that possible unless they were Paid for searches. We just have to come up with a better way to detect these trends. Any Ideas Anyone???
Shawn Mendes Thinks Justin Bieber Could Take Him in a Fight | Elvis Duran Show
Shawn Mendes is preparing for his SNL performance and still isn't sure what he will be doing, we find out who he thinks would win in a fight between him and Justin Bieber. He isn't sure he deserves the Prince of Pop title, and he shares excitement for his new single "If I Can't Have You."
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