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Seen and Heard International
SINCE 1999 A LIVE REVIEW SITE FOR OPERA, BALLET, CONCERTS, BROADCASTS, AND THEATRE
UK Concerts
International Concerts
Opera & Ballet
International Opera
UK Opera
Ballet and Dance Reviews
Broadcasts & Others
Broadcasts and Film
Aspen Festival -2015
Bayreuth-2015
BBC Promenade Concerts 2015
Edinburgh International Festival 2015
Bayreuth Festival 2018
Dresden Music Festival 2018
Edinburgh International Festival and Fringe 2018
Aspen Music Festival 2021
Three Choirs Festival 2021
Musical Perfection from "Finest French Orchestra North of Calais"
22/02/2015 07/02/2015 by Simon Thompson
United Kingdom Franck, Saint-Saëns, Fauré, Ravel: Daniel Müller-Schott (cello), Royal Scottish National Orchestra, Gilbert Varga (conductor), Usher Hall, Edinburgh, 06.02.2015 (SRT)
Franck: Les Éolides
Saint-Saëns: Cello Concerto No. 1
Fauré: Elégie
Ravel: Ma mère l'oye (Mother Goose)
La Valse
In the days when Stephane Denève was their music director, Richard Morrison of The Times described the RSNO as "the finest French orchestra north of Calais." I was reminded a bit of that tonight during Les Éolides with its gossamer-light, shimmering strings, so weightless as to be almost transparent. In fact, tonight's programme was proof that the orchestra's proficiency in French repertoire goes deeper than depending solely on Denève's leadership: they are masters at it under other batons too. Gilbert Varga, who I hadn't come across before, showed a mostly sure touch with a persuasive lilt and an ability to make the bar lines when he needed to. The orchestra responded in kind with playing that glittered like a jewel being held up to the light.
That was brilliantly evident in Ma mère l'oye. If the strings impressed me most in the Franck then it was the winds that were most appealing here. The solos in Sleeping Beauty's opening Pavane were full of wistful nostalgia, and the clarinet and bassoon that played the parts of Beauty and the Beast saw their lines as acting a role, not just notes on a page. Best of all was the orientalism of Laideronette, gleaming and bright from winds and percussion, fully in step with the magic of the piece. Only La Valse struck me as a little false. It was brilliantly played and dazzlingly beautiful in places, but Varga's emphasis on the beauty meant that the final catastrophe came as something of a surprise – not so much a culmination as an out-of-keeping exclamation – and so the ending felt oddly tacked on rather than shocking.
No danger of superficiality with Daniel Müller-Shott on the cello, though it took me a while to tune in to his sound. The tone of his instrument (or was it his style of playing?) was quite astonishingly chocolaty; so sweet as to be almost glutinous, almost a little muffled at times. That carried great benefits in the lower registers, but the opening flourish was indistinct, as if too concerned with effect over substance. He came into his own with the slower second subject, though, which suited his sound brilliantly, and I loved the episode in the central Allegretto when the cello's long-bowed slow theme sits alongside the dainty orchestral minuet. He also took a more thoughtful tone with the finale's slow second theme, which sounded both meditative and communicative at once. Acrobatics were back for the final flourish, but the dispelled again for Fauré's beautiful Elégie which seemed to coarse with songful lyricism and generated a power all of its own.
Categories Concert Reviews, UK Concerts Tags Edinburgh, Simon Thompson Post navigation
A Good Deal of Fine Playing from Anthony Marwood and Aleksandar Madžar
A Flowering Tree Blooms in Gothenburg
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TOP 5 SEEN AND HEARD POSTS
Glorious Lise Davidsen and Leif Ove Andsnes recital of Grieg, Richard Strauss and Wagner
Vienna State Ballet's Liebeslieder is a captivating Jerome Robbins, Lucinda Childs and George Balanchine triple bill
From violent LA to tranquil Malvern Hills: Adams, Elgar, Trevino and the Tonhalle Orchestra Zurich
MTT and Prokofiev's Fifth Symphony – both born in 1944 – join together with the LA Phil
Inspiring Beethoven from Hilary Hahn and Andreas Haefliger at Wigmore Hall
Marc Bridle on On many levels a quite exceptional recital from George Harliono at Wigmore Hall
David Boyce on On many levels a quite exceptional recital from George Harliono at Wigmore Hall
A.L. Hern on An entirely memorable LSO concert of music by Miklós Rózsa and Bartók
Michael on Davide Livermore's cinematic Macbeth with Anna Netrebko at La Scala
Der Ballett Alleswisser on Tamara Rojo's new Raymonda and English National Ballet leadership news
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Real Nitro is a specially chosen mixture of organic ingredients that are formulated specifically to increase muscle mass, speed metabolism, and improve overall athletic performance levels. This muscle increasing supplement includes vital ingredients to nourish muscles so they can perform at their highest levels and safely increase muscle mass, athletic stamina, and reduce recovery time after the gym. Specially formulated to meet the needs of bodybuilders, athletes, and fitness enthusiasts everywhere.
Real Nitro Muscle Supplement is a product of Wellness Nutra LLC, 780 Morosgo Dr. #13111, Atlanta, GA 30324. Wellness Nutra is a Georgia Limited-Liability Company that filed for company status on March 17, 2015. The registered agent listed at this address for this company is Jawara Mayfield.
Real Nitro works by providing increased oxygen levels to the body's muscles by adding to the testosterone levels already present in the blood stream. This increase in oxygen allows the muscles of bodybuilders, athletes, and weightlifters to expand and build mass faster and increase their performance results. The proprietary ingredients in this mass muscle building concoction work together to relax smooth muscles and eliminate unwanted waste and toxins from the athlete's body.
Real Nitro detoxifies the body down to the basic cells, allowing the ingredients to work together to build new superior muscle mass from the cellular level throughout the body. Other ingredients are responsible for increasing the body's blood flow and increasing the athlete's adrenaline levels. All of these ingredients work together in harmony to help create the rock hard body that you desire.
Tribulus Terrestris – This spiny flowering plant from the warm climates of southern Asia, Australia, Africa, and Europe, is an essential component in Real Nitro. It adjusts the levels of testosterone in the body and is a great supplement to an anabolic steroid program. It is responsible for returning the body's testosterone levels to their normal state after a rigorous workout or competition.
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L-Taurine – Acting as a powerful antioxidant, an anti-inflammatory, and blood vessel dilator. This allows for larger amounts and quicker blood flow to the muscles. This increase in blood flow increases the oxygen being delivered to the muscles that are being used when working out or competing. Added oxygen gives the muscles more energy to deliver the most from every rep or set in the gym.
L-Citrulline – This essential amino acid is a byproduct of the production of nitric oxide in the body while exerting physical exercise. This amino acid is essential to increasing the blood flow within the body which delivers more oxygen to the working muscles. This increased oxygen level gives the muscles the boost of energy that they need to push harder and farther than before. It interacts with the body on a cellular level and helps to rejuvenate cells. Another benefit of this ingredient in the Real Nitro proprietary blend is the bonus of having the ability to reduce inflammation in overworked or injured muscles.
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Fadogia Agrestis – The elements found in this ingredient work as a testosterone booster within the body. The extra testosterone that it helps to produce gives the user increased athletic stamina in the gym and for a long time after. It also contributes to elevated moods and an increased overall level of energy and mental focus in competing athletes and lifters. This ingredient will help you to have longer workouts with more positive results both physically and mentally.
Carbon – This nonmetallic chemical element is essential in the forming of alkaloids and amino acids. It is an active and essential component in increasing the body's natural anti-bodies. This completely natural element is effective in the fight against illnesses such as colds and the flu. Having this ingredient in this supplement also helps to protect other vital organs in the body such as the kidneys.
Oxygen – This element is essential to the clear functioning of the blood pumping systems of the body. Oxygen increases the amount of proteins being delivered to the muscles when they are being worked. When the muscles are receiving more oxygen, they are able to work harder and longer, to the benefit of the athlete, lifter, or enthusiast.
Hydrogen – This colorless, odorless, non-toxic, tasteless, nonmetallic element is necessary for the interaction of the other proprietary ingredients in Real Nitro. The special formulation is designed to get the sports athlete and bodybuilder the ripped and chiseled body they are seeking, in a much quicker amount of time with no extra effort.
Nitrogen – This is a natural element that is found in numerous locations naturally. It is an odorless and transparent essential element. It is important in this supplement regarding the processing of other ingredients and delivering antibiotic properties to the body before, during, and after workouts and competitions. It helps the muscles to repair and rejuvenate after being put to the test in the gym or weight room.
Vitamins – There are a number of additional vitamins that contribute to the overall dietary needs of the most active of bodybuilders, athletes, and workout enthusiasts everywhere.
These all natural ingredients in this supplement are perfect for anyone looking to reduce their unwanted fat deposits while increasing their muscle mass, muscle memory, and post-workout muscle recovery. When combined, these ingredients also increase the athlete's workout stamina, raise adrenaline levels, and give the athlete an overall more positive attitude and outlook. When working in harmony with the body, this supplement is responsible for continued fat burning and muscle building even when the athlete is in a resting state.
The advantages of introducing this all natural supplement to a workout program begin at the smallest of levels; the cellular level. Taking this testosterone boosting pill will help to detoxify a body right from the cellular level. This allows for these organic ingredients to enable the body to increase the amount of essential elements it absorbs.
This clears the way to activate and prepare the body's muscles for easy absorption of the increased amount of oxygen, testosterone, and blood flow that these ingredients will produce. Users will experience an increase in muscle mass, lean muscle toning, increased levels of energy, and longer performance stamina for more repetitions in the gym. There will also be a heightened sense of self-worth as well as the melting off of excess pounds while working out and while at rest.
This supplement is certified to be all natural and safe for any consumer when taken in the correct dosage. There is no FDA certification for this product as it is completely organic and safe for consumers. There have been no reports of illness or side-effects associated with this muscle enhancing testosterone boosting supplement.
I started taking Real Nitro about 3 weeks ago and I can see a difference already through my clothes. I have never been able to achieve the bulk in my chest that I wanted until now. I'm no longer embarrassed when up against the other dudes in the gym! Thanks Real Nitro!
I am usually skeptical of products that offer such great results, but after reading the customer reviews, I have to give this free trial a shot. I definitely need to bulk up fast for summer, so I figure why not? It's worth the free trial anyway.
I have been looking for a long time for a way to increase my muscle tone without stuffing my body full of artificial components. I was elated to find this product online. After only 2 weeks of taking this booster, I can physically see a difference in the tightness of the muscles in my chest, and my energy levels are as high as they were when I was 20 years younger. I definitely will be recommending this to the other guys at the gym.
Being a semi-pro ball player, I regularly work out to keep in shape in the off season. Looking to build up some bulk muscle so I can increase my hitting distance, I hit the gym at least five days a week. But I wasn't seeing any increase until I was turned on to your product by another mate in the gym. I am happy to announce that my last practice I was hitting to the track nearly every time and I can't wait to step up to the plate this season.
Real Nitro has been mentioned on a number of credit worthy sites and news medias. Articles and news stories have been featured on such shows as ABC News, on websites such as CNN Health, and in magazines such as Men's Health. The success of this ground breaking mixture of ingredients has captured the attention of bodybuilders everywhere and celebrities such as Dwayne "The Rock" Johnson and Gerard Butler are reported on Men's Health Advice as incorporating this type of supplement in their workout regimen.
The makers of Real Nitro offer a 100% money back satisfaction guarantee on their product. If for any reason the consumer is not completely satisfied with the supplement, sending back the unopened product will result in a complete refund of any price paid. The refund will appear as a credit on the credit card that was initially used for the purchase.
Products must be returned in the original unopened package within 30 days of the initial order. Consumers need to call the customer service department to receive a RMA number for the return package. Shipping and handling costs are not refundable.
The cost of shipping for this trial offer of Real Nitro testosterone enhancing supplement is a low $4.95. Once the account has been established and the trial period is over, there will be a shipping charge of $5.95 per monthly order. There is also a rush shipping option for first time orders. Shipping for this supplement normally takes from 3-5 days.
After the initial free trial is over, if the consumer has not cancelled the order according to the instructions, a new month's supply will be shipped. This shipment will automatically charge the account the cost of a month's supply of the supplement. This will automatically renew every month so that busy athletes and muscle fanatics everywhere have one less thing to worry about.
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The automatic renewal assures that consumers will only have to enter their credit card information one time. Future billings are deducted from the same account once a month until the active account is cancelled by the consumer. All transactions are encrypted and completely safe for you to use for ordering this incredible muscle ripping formula.
The pricing for this incredible muscle builder varies between $81 and $85. This incredible supplement is not available in retail stores and the only way to obtain a bottle of this all natural testosterone boosting muscle enhancer is through their official website. When you visit the web page, you will be invited to an exclusive offer for a free trial of the metabolism boosting ingredients found in Real Nitro.
After the trial period is over for this supplement, Real Nitro will continue to send a new supply of supplement. The first shipment will be sent 45 days after the trial issue has been ordered. After that, there will be a new shipment automatically sent to the consumer every 30 days.
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This incredible muscle enhancing and testosterone boosting supplement is available in a free trial offer. This offer is open to all residents of the United States who are over the legal age of 18 years old who want to bulk up and enhance their physical endurance and stamina on the playing field. There is no cost for this trial above the low cost of shipping the product.
It is that easy to get a free opportunity to increase muscle, raise endurance, increase mental focus, and bulk up quickly and safely at no initial cost or risk upfront. If the customer is satisfied with the trial offer and wishes to continue to receive a monthly delivery of this metabolism crushing supplement, there is no action required. If the order is not cancelled by the consumer within 15 days of the initial order date, it will automatically be renewed and shipped to the indicated address on the original order form.
This supplement comes with a free no-risk trial offer.
Increased levels of adrenaline to increase workout performance.
Comes in a convenient pill form for easy ingestion and transportation in a pocket or gym bag.
Automatic renewal of this supplement each month requires no re-ordering or having to make a special payment.
There are many other benefits to adding these organic ingredients to a workout plan that makes this offer too good to pass up for those looking to sculpt their muscles.
There is a possible side effect if the consumer happens to be allergic to the ingredients in the Real Nitro supplement. This has never been reported, but it is a possibility with an all natural product such as this.
The specifications for the trial offer and return policies need to be taken into account carefully and carried out fully to receive the proper action or refund.
The cons associated with this product are limited to a small amount of billing complaints and does not at all reflect negatively on the performance of this supplement.
Summary: Creating the chiseled and sculptured body that you have always dreamed of is literally just the click of a button away. You can try Real Nitro so you can experience for yourself the advantages this all natural testosterone boosting supplement has for bodybuilders, athletes, weightlifters, and competitors of all types. There is no limit to what you may be able to accomplish when you add this potent blend of proprietary testosterone boosting ingredients to your every day workout plan.


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The Artemis Satellite Team has been awarded the 2003 Space Operations & Support Award at the AIAA's Space 2003 Conference at Long Beach, California (USA).
The Artemis team consists of the European Space Agency (ESA), Alenia Spazio, the prime contractor, Telespazio and Astrium, the company that designed the satellite's ion propulsion system.
The citation reads: "For recovering Artemis and demonstrating the following firsts: Optical inter-orbit satellite link, major on-orbit reprogramming of a telecommunications satellite, orbital transfer to geostationary orbit using ion propulsion, and longest ever operational drift orbit."
The American Institute of Aeronautics and Astronautics (AIAA) is the principal society for the aerospace engineering and scientific community. The Space Operations and Support Award is presented every two years to teams or individuals that make exceptional contributions critical to an anomaly recovery, crew rescue or space failure. This is a prestigious award which is a distinct honour for ESA as well as for the recovery team. Previous awards include the STS-61 Crew, the JPL Galileo Team and the Near Earth Asteroid Rendezvous Team.
The Artemis mission began on 12 July 2001 and was the subject of an unprecedented rescue that spanned 18 months. A malfunction in the upper stage of an Ariane-5 launcher left the satellite in an abnormally low transfer orbit. However, thanks to a combination of advanced technologies on board, coupled with the innovative procedures devised by the spacecraft control team, it was possible to slowly coax the satellite to its intended position.
A team of ESA and industry specialists immediately set to work to rescue the satellite. The shortfall in the satellite's velocity was such that the amount of chemical propellant remaining on board was insufficient. It was decided to employ the satellite's novel experimental ion-propulsion system, originally designed to prevent the satellite from drifting from its working position, to slowly manoeuvre the troubled spacecraft into place.
In all, about 20% of the original spacecraft control software had to be modified in order to accomplish the new mission scenario. Thanks, however, to the re-programmable onboard control concept that had been used on Artemis, remote software patches could be uplinked from the ground. The modified code amounted to a total of 15 000 words, making it the largest reprogramming of flight software ever attempted for a telecommunications satellite.
It was only in January this year that Artemis reached its intended destination, a geostationary orbit some 36 000 km above earth.
"Mission impossible, mission accomplished... Artemis is a great success for the European Space Agency since we have demonstrated our ability both to develop new technologies effectively and also our capabilities to recover a mission that many believed to be lost." Claudio Mastracci, Director of Applications, ESA.


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Borrowing approved for replacement of control room equipment
by John Becker
At Tuesday's meeting, Montgomery County legislators approved bonding for $850,000 to replace the control system at the county's public safety building.
At last week's meeting of the Legislature's Budget and Finance Committee, Undersheriff Justin Cramer and County Executive Matthew Ossenfort told legislators that a lightning strike had disabled equipment that controls locks and doors at the public safety building. The estimated cost of repairing the damage is $840,000. Ossenfort said then that the county had filed an insurance claim, but due to the age of the affected equipment, he did not expect a "substantial" return. Cramer said the equipment was installed in 1995 and is now obsolete, and the company that installed it is no longer in business.
District 9 Legislator Robert Purtell said Tuesday that he had visited the public safety building along with Cramer, Legislature Chairman Terry Bieniek and Sheriff Michael Amato to determine the extent of the damage caused by the lightning strike.
"It certainly is a situation we need to address over there for several reasons: the age of the equipment, the reliability of the equipment, the technology and the condition of it," Purtell said. "It's not clear exactly what caused the problem, but…what happens is that when you get a power outage, whether it's caused by an electrical storm or the power supplier, you have a battery backup system that takes over for 10 seconds [before the emergency generator comes on]."
"All the batteries are old, the equipment is old, the computer equipment that's running the system is from 1998, and they look like CPUs that you would have had back in 1998. The mechanisms in the control boards are not functioning as properly as they should."
Purtell said he felt comfortable supporting the bonding resolution, even though it meant putting off another capital project–replacing the roof on the public safety building-for another year or two.
Legislators asked whether the amount received from an insurance claim should be deducted from the amount bonded for the project. County Attorney Megan Manion said the bond could be paid off early using the proceeds from the insurance claim. Purtell suggested using any extra money to replace worn locking mechanisms in the building. No decision was made on what to do with any extra money.
John Becker
John Becker is both a Reporter and Consulting Editor for The Compass. He and his wife Pat operate Abbey Farms in Amsterdam NY.


I ought to be in Asia not less than after New Years so we are able to meet up if we're each in BKK at the similar time. In the meantime, let me know if you have some other questions! Payroll companies supplied by ADP®, Inc. Chase could obtain a referral payment and neither guarantees nor makes any representations or warranties of any sort relating to the services. The ADP® emblem is a registered trademark of ADP®, Inc. Some consumer names make individuals surprise, What were they thinking???" When you do not need to elicit that kind of response from potential buyers, then select a name that people won't find offensive, will not be sexually suggestive, and would not convey a adverse connotation or destructive angle. Try eBay's pointers to be taught their standards of what's and isn't allowed in person names.
That is why I like to recommend the Mastering Influence Program It is the simplest solution to shut any human being who is closeable. There isn't a pressure concerned for either the buyer or vendor, and it is not so much like promoting as it is taking somebody from one level to another in a logical resolution-making process. While Amazon has an extensive collection of products, almost 50% are actually provided by third get together sellers. The website experience is among the greatest with sturdy security, detailed product critiques and the all the time standard amazon reward card that enhances an already robust stream of visitors to the positioning, making it ideally suited for sellers.
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Tuesday, January 19, 2016 @ 11:30 a.m.
Tuesday, January 26, 2016 @ 5:30 p.m.
Wednesday, January 13, 2016 @ 5:30 p.m.
Wednesday, January 27, 2016 @ 11:30 a.m.
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Thursday, January 28, 2016 @ 11:30 a.m.
Tuesday, January 12, 2016 @ 5:30 p.m.
Thursday, January 14, 2016 @ 11:30 a.m.
How To Get More Money From Social Security.
You Should NOT Be Private Paying For Nursing Home Care.
To Stay In My Own Home?
Now What? My Spouse Can No Longer Take Care Of Me.
Protect Your Home From Being Sold To Pay For Nursing Home Care Costs.
Keep The Nursing Home From Taking Your Children's Inheritance.
The "Frozen Asset Rule" & What It Means For You.
Better To Have Planned Ahead & Save More.
Thursday - December 17, 2015 @ 12:35 p.m.
What Assets Of Mine Would Be At Risk To Spend On Nursing Home Care Costs?
What If My Spouse Becomes Incompetent?
What Assets Would Be Left For Our Children To Inherit?
Plan Ahead And Save More.
All Phone Seminars are recorded for future use. If you are unable to listen at the time of the live Phone Seminar or are interested in a past Phone Seminar topic, just click here to choose from a variety of past recorded Phone Seminars you can listen to.
This article explores why an attorney should be alert to undue influence their client may be under and why a caregiver should aware and alert to any undue influence that those they are giving care to may be under.
The Centers for Medicare and Medicaid has announced the 2016 Medicare premiums, deductibles, and coinsurances . . .
The federal budget agreement that President Obama signed into law November 2, 2015, contains changes to how spouses can claim Social Security benefits, ending two strategies previously used to maximize benefits.
If you create a trust, you will need a separate person or institution, called a "trustee," to manage the trust either now or in the future, depending on the type of trust.
Most long-term care involves assisting with basic personal needs rather than providing medical care.
Unsubscribe | This newsletter is from Attorney Timothy P. Crawford S.C.


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Mary J. Blige Teams Up with LL Cool J's Wife for Jewelry Collaboration
R&B queen Mary J Blige has teamed up with jewelry designer Simone I Smith for Sister Love, a futuristic jewelry collaboration, that launched on Monday (July 9).
Smith is the wife of LL Cool J and started her eponymous jewelry collection in 2011 after undergoing surgery for stage III Chondrosarcoma, a rare bone cancer. In a press release obtained by EBONY, the Yonkers native explained why she partnered with Smith for her move into the jewelry industry "What made me want to work with Simone is she already created an amazingly successful jewelry line and all the pieces are things that I love, and she also has an amazing fashion sense," said Blige "I wanted to add to that by sharing some of my fashion sense with her and creating some things I dream about and I know other women are dreaming about" Just as the name suggests the collaboration pays homage.	Source


Seriously Daisies: My Creativity Returns!
I am so happy! And so is my nagging "you need to get back to work" husband! I finished one of my custom scrapbook page orders for Jen. And, I also made a little something to post on eBay. A really cute little ballerina tear bear. Making Lemonade Design Team is having a "Think Pink" challenge for the entire month of October. We are donating 10% of all of our "pink auction" totals to The Susan G Komen Foundation. I am so happy to finally be contributing to our groups efforts on eBay, and am thrilled to be creating stuff again. Yay! Go me!!!!!


text:
TPO1, a member of a novel protein family, is developmentally regulated in cultured oligodendrocytes
W. H H Krueger, G. E. Gonye, Dana Madison, K. E. Murray, M. Kumar, N. Spoerel, S. E. Pfeiffer
Although the myelin membrane contains only a small set of major proteins more sensitive assays indicate the presence of a plethora of uncharacterized proteins. We have used an antibody perturbation approach to reversibly block the differentiation of prooligodendroblasts into myelinating cells, and, in combination with a differential screening procedure, identified novel mRNAs that are activated during this period. One cDNA, TPO1, recognizes a 5.5-kb mRNA that is strongly up-regulated in oligodendrocytes after release of the differentiation block and that is expressed at high levels in brain tissue during active myelination. This cDNA represents at least two mRNAs differing from each other in their 5'-termini. The TPO1 cDNA contains an open reading frame of 1,380 bp, encoding a protein of 51.8 kDa with a predicted pl of 9.1 that contains two regions homologous to nonclassic zinc finger motifs. Subcellular localization studies suggest the enriched presence of TPO1 n spherical structures along the major cytoplasmic processes of oligodendrocytes. TPO1, along with homologues expressed in testis, placenta, and PC12 cells, form a novel family of proteins with multiple hydrophobic domains possibly serving as membrane spanning regions. We postulate that in oligodendrocytes, TPO1 encodes a protein factor involved in myelin biogenesis.
Journal of Neurochemistry
Open Reading Frames
Membrane protein
OB- fold
Oligodendrocyte development
TPO1
Zinc finger
Krueger, W. H. H., Gonye, G. E., Madison, D., Murray, K. E., Kumar, M., Spoerel, N., & Pfeiffer, S. E. (1997). TPO1, a member of a novel protein family, is developmentally regulated in cultured oligodendrocytes. Journal of Neurochemistry, 69(4), 1343-1355.
TPO1, a member of a novel protein family, is developmentally regulated in cultured oligodendrocytes. / Krueger, W. H H; Gonye, G. E.; Madison, Dana; Murray, K. E.; Kumar, M.; Spoerel, N.; Pfeiffer, S. E.
In: Journal of Neurochemistry, Vol. 69, No. 4, 10.1997, p. 1343-1355.
Krueger, WHH, Gonye, GE, Madison, D, Murray, KE, Kumar, M, Spoerel, N & Pfeiffer, SE 1997, 'TPO1, a member of a novel protein family, is developmentally regulated in cultured oligodendrocytes', Journal of Neurochemistry, vol. 69, no. 4, pp. 1343-1355.
Krueger WHH, Gonye GE, Madison D, Murray KE, Kumar M, Spoerel N et al. TPO1, a member of a novel protein family, is developmentally regulated in cultured oligodendrocytes. Journal of Neurochemistry. 1997 Oct;69(4):1343-1355.
Krueger, W. H H ; Gonye, G. E. ; Madison, Dana ; Murray, K. E. ; Kumar, M. ; Spoerel, N. ; Pfeiffer, S. E. / TPO1, a member of a novel protein family, is developmentally regulated in cultured oligodendrocytes. In: Journal of Neurochemistry. 1997 ; Vol. 69, No. 4. pp. 1343-1355.
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abstract = "Although the myelin membrane contains only a small set of major proteins more sensitive assays indicate the presence of a plethora of uncharacterized proteins. We have used an antibody perturbation approach to reversibly block the differentiation of prooligodendroblasts into myelinating cells, and, in combination with a differential screening procedure, identified novel mRNAs that are activated during this period. One cDNA, TPO1, recognizes a 5.5-kb mRNA that is strongly up-regulated in oligodendrocytes after release of the differentiation block and that is expressed at high levels in brain tissue during active myelination. This cDNA represents at least two mRNAs differing from each other in their 5'-termini. The TPO1 cDNA contains an open reading frame of 1,380 bp, encoding a protein of 51.8 kDa with a predicted pl of 9.1 that contains two regions homologous to nonclassic zinc finger motifs. Subcellular localization studies suggest the enriched presence of TPO1 n spherical structures along the major cytoplasmic processes of oligodendrocytes. TPO1, along with homologues expressed in testis, placenta, and PC12 cells, form a novel family of proteins with multiple hydrophobic domains possibly serving as membrane spanning regions. We postulate that in oligodendrocytes, TPO1 encodes a protein factor involved in myelin biogenesis.",
keywords = "Membrane protein, Myelin, OB- fold, Oligodendrocyte development, TPO1, Zinc finger",
author = "Krueger, {W. H H} and Gonye, {G. E.} and Dana Madison and Murray, {K. E.} and M. Kumar and N. Spoerel and Pfeiffer, {S. E.}",
journal = "Journal of Neurochemistry",
T1 - TPO1, a member of a novel protein family, is developmentally regulated in cultured oligodendrocytes
AU - Krueger, W. H H
AU - Gonye, G. E.
AU - Madison, Dana
AU - Murray, K. E.
AU - Kumar, M.
AU - Spoerel, N.
AU - Pfeiffer, S. E.
N2 - Although the myelin membrane contains only a small set of major proteins more sensitive assays indicate the presence of a plethora of uncharacterized proteins. We have used an antibody perturbation approach to reversibly block the differentiation of prooligodendroblasts into myelinating cells, and, in combination with a differential screening procedure, identified novel mRNAs that are activated during this period. One cDNA, TPO1, recognizes a 5.5-kb mRNA that is strongly up-regulated in oligodendrocytes after release of the differentiation block and that is expressed at high levels in brain tissue during active myelination. This cDNA represents at least two mRNAs differing from each other in their 5'-termini. The TPO1 cDNA contains an open reading frame of 1,380 bp, encoding a protein of 51.8 kDa with a predicted pl of 9.1 that contains two regions homologous to nonclassic zinc finger motifs. Subcellular localization studies suggest the enriched presence of TPO1 n spherical structures along the major cytoplasmic processes of oligodendrocytes. TPO1, along with homologues expressed in testis, placenta, and PC12 cells, form a novel family of proteins with multiple hydrophobic domains possibly serving as membrane spanning regions. We postulate that in oligodendrocytes, TPO1 encodes a protein factor involved in myelin biogenesis.
AB - Although the myelin membrane contains only a small set of major proteins more sensitive assays indicate the presence of a plethora of uncharacterized proteins. We have used an antibody perturbation approach to reversibly block the differentiation of prooligodendroblasts into myelinating cells, and, in combination with a differential screening procedure, identified novel mRNAs that are activated during this period. One cDNA, TPO1, recognizes a 5.5-kb mRNA that is strongly up-regulated in oligodendrocytes after release of the differentiation block and that is expressed at high levels in brain tissue during active myelination. This cDNA represents at least two mRNAs differing from each other in their 5'-termini. The TPO1 cDNA contains an open reading frame of 1,380 bp, encoding a protein of 51.8 kDa with a predicted pl of 9.1 that contains two regions homologous to nonclassic zinc finger motifs. Subcellular localization studies suggest the enriched presence of TPO1 n spherical structures along the major cytoplasmic processes of oligodendrocytes. TPO1, along with homologues expressed in testis, placenta, and PC12 cells, form a novel family of proteins with multiple hydrophobic domains possibly serving as membrane spanning regions. We postulate that in oligodendrocytes, TPO1 encodes a protein factor involved in myelin biogenesis.
KW - Membrane protein
KW - Myelin
KW - OB- fold
KW - Oligodendrocyte development
KW - TPO1
KW - Zinc finger
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry