It is no secret that I am crazy for weddings. When allowed to create their wedding flowers, I am privileged to share one of the most joyful days in the lives of a couple. This year was over-the-top for me as SIX of our current or former Kate's Garden staffers got married in 2017. And guess who got to do the flowers? My cup runneth over. John, our two Lauras, Shirley, our son Evan and Madisyn all tied the knot and each of them chose our Kate's GardenDIY Wedding "Posse" approach for their respective weddings.
Our DIY Wedding Posse service is unique here in the GTA but it is actually common in England. We've offered this service for over fifteen years and the concept is simple. The bride (or groom) is allowed to bring along a gang of up to four helpers to the teaching room of our Main Street Markham shop. They become instant floral assistants, are provided step-by-step guidance and assistance by our talented floral team and use all of our equipment for free. Our posses enjoy a big advantage in terms of learning and creative control with significant savings as an added bonus.
Laura E. kicked it off in February with a wintry evergreen theme for her wedding to David at The Doctor's House, an historic venue in the heart of Kleinburg. Laura is depicted here with her posse.
John married his smiling Marissa this past June. The beautiful country setting of The Herongate Barn Theatre was the perfect venue for their rustic chic wedding. We added celtic flair to the bouquets of daisies, lisianthus and hydrangea by interspersing tartan leaves and the button cuff with ribbon from their Scottish clans. Marissa is depicted here with her new mother-in-law Bernadette.
Last year, our long-time employee Laura M. moved to Prince Edward Island to marry her "Gilbert". (If you love Anne of Green Gables as much as I do, you will know what it means to meet your Gilbert. Laura found her perfect match, kindred spirit and soul mate in Sheldon). Talented photographer and artist Laura and Sheldon, pastry chef extraordinaire styled a whimsical, outdoor wedding that took place on a perfect island day in Tignish. My husband Jim and I loved helping with the wedding prep, event styling and flowers. Alberton Florist was kind enough to procure the patience garden roses, blush astilbe, blue thistle, pinks alstros, seeded eucalyptus and tiki fern. Jim and I also enjoyed one of the most delicious wedding banquets ever with lobster and veggies and PEI potatoes fresh from Sheldon's family farm; the Clohossey Farm. The meal was topped off by a scrumptious wedding cake created by the groom himself.
Our social media guru Shirley married Michael on a gorgeous Sunday afternoon recently at McLean House at The Estates of Sunnybrook. To match the charming garden setting and historic ambience, Shirley's posse created bouquets that looked freshly picked. It was the perfect setting for their dog Tibby the Corgi to make her entrance. Tibby, Toronto's most popular dog with a website and over 250000 followers, had a collar made of spray roses and salal tips.
Our blogging expert Madisyn runs a blog called The Restless Worker which is all about millennial travel. Madisyn and Ben's blog inspired their travel-themed wedding which took place on the rooftop of the ultra chic Thompson Hotel on a recent Friday night. Madisyn's family and friends posse had an amazing time creating the flowers for the wedding and reception.
As an aside, for the past few years, many of our weddings have been on Fridays and Sundays. Savvy brides and grooms know that alternate dates are appreciated by guests and sometimes offer price-savings as well. Friday night weddings are a personal favourite as they seem to both more relaxed and party-oriented; giving a great kick start to weekends. Though more sedate, Sunday afternoon weddings are genteel and charming.
For me, the icing on the most delicious wedding cake ever took place at the end of July when our son Evan married his darling Joanna. Their cake is called the Signature Mille Crêpes created by Lady M at World Trade Centre 4; unbelievably delicious. Our wonderful son Evan married his darling Joanna at an intimate, stylish wedding in New York City. We kicked off our NYC floral adventure by visiting the New York Florist Market; a must see for flower lovers located in the heart of the city. Ubering home our glorious array of hot-coloured flowers (including mini-hellebores!) and fantastic foliage was actually easy. It was a treat creating the flowers for their Tribeca wedding and rooftop reception followed by a mouth-watering dinner at neo-bistro and natural wine bar Rebelle in Nolita.


To search for over represented tran scription factor binding sites in the DEGs induced by HDACIs, we used a web based selleck chemical Ixazomib program CORE TF. This program was used to search for common Inhibitors,Modulators,Libraries TF binding motifs, derived from postion based matrices from the TRANSFACR Inhibitors,Modulators,Libraries database. The search for TFBS was restricted to the 1000 bases upstream of the tran scription start site. The output p values and promoter hits were obtained after correcting for a false discovery rate of 1%. The methods have been detailed previously. Ingenuity pathways analysis The canonical network models of DEGs were developed using the IPA lined in detail previously. The Illumina gene lists were uploaded as a text file and each gene identifier was mapped to its corresponding gene object.
An initial gene set of DEGs was first overlaid onto the set of all catalo gued interactions and Inhibitors,Modulators,Libraries focus genes contained in the IPA library of canonical pathways. To start building net works, the application queries the Ingenuity Pathways Knowledge Base for interactions between Focus Genes and all other gene objects stored in the knowledge base and generates a set of networks each with no more than 35 genes/proteins. The IPA then computes a score for each network according to the fit of the users set of sig nificant genes. The score is derived from a p value that denotes the likelihood of a Focus Genes presence in a network due to chance. The networks graphically denote nodes and edges, or lines. Assignment of nodes in gene net work is made using published observations stored in the Ingenuity Pathways Knowledge Base.
A Fischers exact test was used to calculate a p value predicting the prob ability that the biological function assigned to that net work is explained by chance alone. PCR based quantification of gene expression RNA was extracted from Inhibitors,Modulators,Libraries control or treated H9c2 cardiac myocytes using TRIzol RNA extraction reagent. Total RNA was precipitated with ethanol, concentrated by centrifugation and dissolved in diethylpyrocarbonate treated water. Aliquots of 800 ng of RNA were used to synthesize cDNA. Gene specific primers and Taq Man probes for quantitative RT PCR were designed using Universal Probe Library as detailed previously. The Cp values for each HDAC and Sirtuin gene were normalized to the Cq values of the constitutively expressed actin gene.
Western blot analysis Total proteins from H9c2 cells were extracted using radio immunoprecipitation buffer Inhibitors,Modulators,Libraries according to the manufacturers protocol. The nuclear and cytoplasmic and fractions were separated using the NE PERTM method. For western blot analysis, equal amounts of protein from each sample were separated using 10% SDS PAGE. After electrophoresis, MK-8745? the protein samples were transferred to Immobilon P membranes using a Trans Blot elec trophoresis transfer cell. Various HDACs, sirtuins and MAP kinases were detected on western blots with mono specific primary antibodies.
The lamellum adheres to the ECM, provides a broad surface for traction, and contains a network of actin filaments, like that seen in untreated rat microglia. selleck We found that Inhibitors,Modulators,Libraries the morphology and cytoskeletal arrangement of microglia was profoundly affected by LPS, and more subtly affected by IL4. LPS treated cells were ameboid or rounded up, and had many vinculin rich Inhibitors,Modulators,Libraries and F actin rich filopodia without a specific orientation. This is consistent with previous descriptions Inhibitors,Modulators,Libraries of LPS activated microglia. In con trast, most resting and IL4 treated microglia had a pola rized morphology, with a lamellum at the front and a uropod at the rear. In earlier work, IL4 changed rat and mouse primary microglia from rounded or ameboid to a more ramified shape, with processes and la mellipodia.
However, we found that the lamellum of IL4 treated cells was smaller and exhibited more membrane ruffles, Inhibitors,Modulators,Libraries and both the lamellum and uropod showed ex tensive co localization of F actin and vinculin. Changes in actin distribution and polymerization underlie the morphological polarization and roles of both the lamellum and the uropod. Precise roles of the uropod in cell migration are unknown but it is considered im portant for cells that migrate through tight spaces. The presence of a uropod and lamellum in rest ing and alternatively activated microglia suggests that these cells will migrate well through the tightly packed brain parenchyma during development and after CNS injury. A hallmark of polarization in migrating cells is coordi nated reorientation of the NC axis.
In many migra ting Inhibitors,Modulators,Libraries cells, the nucleus moves toward the rear, resulting full read in an anterior NC axis in which microtubules oriented toward the leading edge are stabilized. The MTOC, endoplasmic reticulum and Golgi apparatus are then in front of the nucleus. Many cells display an anterior NC orientation when migrating on 2 D substrates for ex ample, macrophages, neurons, astrocytes, and epithelial and mesenchymal cells. The opposite posterior NC orientation is less common but seen in some migrating immune cells, especially neutrophils and T lymphocytes. The precise role of the MTOC position in cell migration is unknown however, it can be affected by extracellular cues. For in stance, neutrophils changed their MTOC orientation to an anterior position during chemotaxis, and to a dorsal position near the cell surface after exposure to an antigen antibody complex. MTOC repositioning during non migratory events includes re orientation to ward phagosomes in macrophages and toward the immune synapse in bone derived dendritic cells. Neutrophils are especially interesting because they are one of the fastest moving mammalian cells, and ex hibit a variable MTOC orientation during random mi gration on glass or formvar.
Indeed, this data showed that activation of CD40 in presence of CD3CD28CpG re duced IL10 and increased IL30 in all the tested time points, therefore suggesting that activation of CD40 during the combination treatment acts as a molecular rheo stat in the regulation of IL10 and IL30. Our previous study shows that Ixazomib MLN2238 CD3CD28CpG in duces IL 30, and this study shows that the same stimuli induce IL10 expression. An intriguing question is whether IL 10 regulates IL30 via the CD3CD28CpG combination. To answer this question, splenocytes were treated with the combination signal in the presence or absence of IL 10. In the presence of recombinant IL 10, IL 30 expression levels induced by CD3CD28CpG were significantly inhibited, suggesting that IL 10 negatively Inhibitors,Modulators,Libraries regulates IL 30 expression.
Additionally, in IL 10 splenocytes, CD3CD28CpG induces higher levels of IL 30 when compared to wildtype counterparts. Inhibitors,Modulators,Libraries These data suggest that the combination treatment induces high levels of both IL 10 and IL 30, but IL 10 also serves as a negative inhibitor of IL 30. However, recombinant IL30 boosts IL10 levels in a dose dependent manner. Ligation of CD40 inhibits IL 10 upregulation by CD3CD28CpG, while it promotes IL 30 expression. This observation pro vokes the question whether IL 10 or activation of CD40 signaling depend on each other to alter IL 30 expression. Indeed, even in the presence or absence of IL 10, anti CD40 stimulation upregulates IL 30 expression. These results suggest that both IL 10 and CD40 can regu late IL 30 expression via two separate mechanisms.
The activation of the CD40 pathway via the agonist antibody lowers IL 10 induction while the activation of NF B1 and STAT3 raises IL 10 expression. One possibility is that Inhibitors,Modulators,Libraries the activation of CD40 lowers NF B1 or STAT3 ac tivation over time, therefore affecting IL 10 expression. To test this hypothesis, splenocytes were treated in the absence or presence of agonist anti CD40 antibody over time and probed for the phosphor STAT3 or NF B1 p65, or NF B p50105. There was no difference in the levels of STAT3 activation or the total Inhibitors,Modulators,Libraries levels of NF B p50105 by the engagement of CD40 in splenocytes. We also tested whether these specific transcription factors were upregulated in specific cell populations, such as CD4 T cell or macrophages. Indeed, no differences in the levels of p p65 or pSTAT3 were observed between samples treated with CD3CD28CpG in either the presence or absence of CD40.
Additionally, even in absence of NF B1 signaling, anti CD40 can further inhibit Inhibitors,Modulators,Libraries IL 10 expression levels. Because IL10 and IL30 also regulate each other and CD40CD154 acts a rheostat in the regulation of IL10 or IL30, one possibility is that these cytokines affect the CD40CD154 pathway. www.selleckchem.com/products/Temsirolimus.html To test this hypothesis, splenocytes were treated with rIL10 or rIL30 and the levels of CD154 in CD4 T cells and CD40 levels on macrophages were tested.
In monocul tures, PC3 cells exhibit profiles consistent with sellectchem a highly metastatic phenotype, while HS5 cells expressed profiles consistent with a non cancerous mesenchymal Inhibitors,Modulators,Libraries phenotype. When co cultured with PC3 cells, HS5 cells re expressed N Cadherin and CXCR7, proteins that are known to ac celerate cancer growth at the primary tumour site and support metastatic colonisation in distal organs. One of the hallmarks of EMT is the loss of the E Cadherin, and the concomitant increase in expression of the mesen chymal cell cell adhesion molecule N Cadherin, a process also known as the Cadherin switch, which can pro voke cell migration and invasion in breast cancer cells. Here we report for the first time that soluble factors excreted by PC3 cells can alone mediate the re expression of N Cadherin in HS5 cells.
Functionally, this up regulation Inhibitors,Modulators,Libraries is known to cause a change in the adhesive properties Inhibitors,Modulators,Libraries of cells and in the case of tumour cells, lose their affinity for their epithelial neighbours, a mechanism that encourages metastatic seeding and colonisation. Further studies are now needed to verify the identity of these soluble molecules responsible for this up regulation in N Cadherin and the direct functional consequences of these alterations. A large number of growth Inhibitors,Modulators,Libraries factors and their activated signal transduction pathways are known to provoke the Cadherin switch including transforming growth factor B, hepatocyte growth factor, insulin like growth factor, Inhibitors,Modulators,Libraries fibro blast growth factor and Notch signalling.
In addition to soluble factors, there are a plethora of contact mediated variables that could account for the re expression of CXCR7 in co cultured HS5 cells. One possibility is the modulation of chemokine receptors through hypoxic conditions, which is known to induce cancer cell expression of c Met, the bona fide receptor of HGF, and CXCR4, the signalling receptor selleck chemicals Perifosine of the chemo kine CXCL12, and further stimulate cancer cell migration and dissemination. Alpha 6 and B1 integrins mediate EMT proteins and CXCR7 expression in co cultures We report here that both 6 and B1 integrin subunits can influence expression rates of important EMT markers and chemokine receptor CXCR7 in both monocultured and co culture assays. Our results regarding integrin mediated changes in these proteins is summarised in Figure 7B. Taken to gether, our results suggest that inhibition of 6 and B1 integrins can mediate a MET program in monocultured cells, while integrin mediation in co cultures is clearly altered with the re establishment of functional N Cadherin and vimentin expression on PC3 cells, consistent with an EMT program.
Considerable evidence supports its role in extracellular pyrophosphate transport. ePPi is a key regulator of pathologic mineralization in cartil age and other tissues. ePPi can be generated from eATP through the action of ecto enzymes with nucleoside tri phosphate pyrophosphohydrolase Rucaparib IC50 activity, such as ENPP1. Because there is ample ENPP1 activity in normal cartilage to convert all available NTP to NMP and PPi, substrate availability is the rate limiting Inhibitors,Modulators,Libraries step in this reaction. We recently demonstrated that chon drocyte eATP and ePPi elaboration were coordinately regulated, supporting a major role for eATP in ePPi production by cartilage. Thus, delineating mechanisms of eATP efflux in cartilage may lead to the identification of novel modulators of ePPi production.
Whether ANK itself may act as an ATP transporter in chondrocytes is not known. Our initial studies involved stable over expression of ANK, but did not investigate whether over expression could Inhibitors,Modulators,Libraries indirectly increase ATP efflux, for example, by altering the chondrocyte phenotype or affecting levels of eATP metabolizing ecto enzymes. Structural studies of ANK protein make it unlikely that ANK itself, at least in its monomeric form, is capable of providing a channel of adequate size to accommodate ATP. Thus, the possibility that ANK regulates a known mechanism of cel lular ATP export warrants investigation. Four classic ATP membrane transport mechanisms have been described to date. Hemichannels, composed of ei ther connexin or pannexin proteins, mediate ATP release in many cell types and have been implicated in chondro cyte ATP efflux.
Vesicular transport of ATP is best characterized in nerve cells, where ATP is packaged along with other neurotransmitters Inhibitors,Modulators,Libraries for rapid release upon Inhibitors,Modulators,Libraries cell activation. Vesicular transport of ATP has also been observed in osteoblasts. Two types of molecularly undefined ATP transport channels also exist. Maxianion channels are typically identified by patch clamp experi ments, and can be inhibited by anion transport inhib itors and gadolinium. Volume sensitive outwardly rectifying anion channels or volume sensitive organic osmolyte and anion channels are widely expressed channels that rapidly develop after cell swelling.
While pharmacologic inhibitors are often used to differen tiate between various ATP release Inhibitors,Modulators,Libraries mechanisms, interpre tations of inhibitor experiments are complicated by considerable overlap in the actions of these agents and anomalous inhibitor responses when multiple transport mechanisms are present in one cell type. The ionotropic P2X purinergic receptors, P2X7 animal study and P2X4, have also been implicated in eATP release. These complex receptors respond to stimuli by rapidly opening cation channels and initiating cell signaling. In many cell types, P2X7 and P2X4 receptor channels also comprise or regulate pores capable of transporting mole cules as large as 900 Da.
On average, selleck chemicals treatment with 10% serum for 48 hours resulted Inhibitors,Modulators,Libraries in a six fold increase in the total number of cells in the wound and in the proliferated cells at both the edge and in the wound. In addition, there was an effect of time in the outer zone cells, with the total number of cells and the proliferated cells in the wound being greatest at 48 hours. No differences were detected in cells that migrated but did not prolifer ate in the wound of outer zone cells. The effects of IL 1 on inner and outer zone micro wound repair IL 1 treatment of meniscal cells from the inner or outer zones resulted in decreased accumulation of proliferated cells in the micro wound. As compared to the inner zone control at 48 hours, the overall total number of cells in the wound Inhibitors,Modulators,Libraries and the pro liferated cells in the wound were significantly decreased by IL 1 treatment.
However, 0. 1 ng mL IL 1 at 48 hours showed an increase in the total cells in the wound, as compared to all other treatments at 24 hours, and a corresponding increase in the number of cells that migrated but did not proliferate in Inhibitors,Modulators,Libraries the wound, as compared to all other treatments at both 24 and 48 hours. There was a signifi cant increase in the number of migrated cells in the wound at 48 hours in the 1 ng mL and 10 ng mL IL 1 treatment groups. Overall for inner zone cells, the control treatment caused the greatest proliferation at the edge and in the wound and decreased the number of cells that migrated but did not proliferate in the wound. There was also an effect of Inhibitors,Modulators,Libraries time, with 48 hours showing increased total cells, proliferated cells and migrated cells in the wound, as compared to the 24 hour time point.
In the outer Inhibitors,Modulators,Libraries zone meniscal cells, IL 1 treatment caused a significant decrease in the number of prolifer ated cells in the wound, as compared to control. However, IL 1 did not have a significant effect on the total cell numbers in the wound, migrated cells in the wound, or the proliferated cells at the edge in the outer zone meniscal cells. The effects of TNF a on inner and outer zone micro wound repair Meniscal cells from the inner, but not the outer zone, showed diminished accumula tion of proliferated cells in the micro wound with increasing concentrations of TNF a. In the inner zone cells, proliferation at the edge was diminished by all concentrations of TNF a, as com pared to control. In addition, the 1 and 10 ng mL con centrations of TNF a caused significant decreases in proliferation at the edge, as compared to 0. 1 ng mL TNF a. At 48 hours, proliferation in the wound was significantly higher than at 24 hours. In the inner zone cells treated compound libraries with TNF a, there were no differences in the total cells in the wound or migrated cells in the wound.
We next ranked the genes based on their MG132 DMSO mRNA, pro tein expression correlation, and then grouped them into pentiles and compared the distribution of BP by pentile. Across the five pentiles gene expression regulation was the most dominant BP, the next two big gest BP groups, consistent across the five pentiles, were proliferation and cell cycle. Both proliferation and cell cycle are central to lymphoblastoid cell physiology and neoplastic transformation. The proliferation, cell cycle and proliferation, PCD ratios were both 4. 5 in pentile 1. In contrast the mean ratios for the other four pentiles were 1. 4. The high correlation between mRNA and protein expression, coupled with predomin ance of genes involved in cell proliferation in pentile 1, suggested that pentile 1 genes may be transcriptionally regulated via Meq and this would favor neoplastic transformation.
We next identified the numbers of putative canonical MDV Meq binding sites in each of the 88 concordantly expressed genes promoters as described. Genes in pen tile 1 have more Meq binding Inhibitors,Modulators,Libraries sites in their promoters than those in the other pentiles, which do not differ from each other. Of the five concordant genes previously implicated in Inhibitors,Modulators,Libraries lympho magenesis in other species, BRCA2, CD30, CD40LG, and PENK are in pentile 1 with a group mRNA,protein expression correlation of 0. 92, suggesting direct transcriptional regulation by Meq. In contrast, CST3 is in pentile 4 with a large decrease in protein but small decrease in mRNA.
It is possible that CST3 is regulated at the level of miRNA, an alternative possibility is that CST3 is a secreted protein so a small decrease Inhibitors,Modulators,Libraries in mRNA could result in a large decrease in cellular protein and, consistent with our observation, most Inhibitors,Modulators,Libraries CST3 was located in the predominantly soluble differential detergent frac tion 1. Notably, IRG1 was in Inhibitors,Modulators,Libraries pentile 1, and has the most Meq binding sites of all the concordant genes, all of which are MERE II binding sites, suggesting Meq induced transcriptional repression, and a central role in MD neoplasia. Overall, the data suggests that the genes in pentile 1 are critical for neoplastic transformation. miRNAs are non coding post transcriptional selleck chemical repres sors potentially important in neoplasia and we identified 152 expressed chicken miRNAs. Of get several genes associated with neoplastic processes, gga mir 204 targets FAS apoptosis in hibitory molecule 2, RAB22A and HDAC 9, gga mir 489 targets FAS asso ciated factor 1 and gga mir 7 targets RAS related viral oncogene homolog 2. Except FAF1 none of these proteins were identified and so we cannot confirm the upregulated miRNAs potential effects on neoplasia in CD30hi cells. Notably however, gga mir 183 which targets EZR mRNA, was decreased and EZR protein increased, i. e.
178 uM to 400 uM and fur ther incubated for 48 hrs or 72 hrs. At http://www.selleckchem.com/products/INCB18424.html least Inhibitors,Modulators,Libraries 4 6 hrs before the end of treatment time, presto blue reagent Inhibitors,Modulators,Libraries was added and incubated for total of 48 or 72 hrs and fluorescence measured. DMSO concentration was corrected to 1% in all wells. Vehicle treated control cells were considered as 100% viable against which treated cells were compared. Experiments were performed in triplicate. Data was expressed as mean SD of triplicate experi ments. Dose response curves to calculate IC50 values were plotted using Graph Pad Prism Software. In order to ascertain role of ROS in BT induced cyto toxicity, we performed cell viability assays in the presence of an antioxidant, ascorbic acid. Cells were pre treated with 1 mM ascorbic acid for 2 hrs before addition of drug and further incubated for 48 hrs with both BT and ascorbic acid.
Inhibitors,Modulators,Libraries Restoration of cell viability was analyzed. An additional cell viability assay was performed in order to assess role of p38 activation in BT induced cytotoxicity, in presence of the p38 inhibitor SB203580. Cells were treated with BT in presence of 10 uM SB203580 for 48 hrs and cell viability Inhibitors,Modulators,Libraries was determined. Lastly, to test if Akt inactivation is essential for drug sensitivity in ovarian cell lines treated with BT, a third cell viability assay was performed in order to see if additional pAkt inactivation would further enhance the effectiveness of BT. To look at this, we treated cells with BT in presence or absence of the pAkt inhibitor LY294002. Lactate dehydrogenase assay LDH release was measured using CytoTox One Homo genous Membrane Integrity kit following the manufacturers instructions.
Briefly, Inhibitors,Modulators,Libraries 10 103 cells 100 uL were plated per well of the 96 well plate and treated with different concentrations of BT ranging from 12. 5 uM to 400 uM for 6, 24 and 48 hrs. Following treat ment, 100 uL of CytoTox One reagent was added to each well. After incubation for 10 min at room temperature, the fluorescence intensity was measured using a fluorescence microplate reader, Fluoroskan. A maximum LDH release control set was gener ated as reference to calculate the actual %LDH release from each sample. Percent of LDH released from vehicle treated control set is considered as 100% intact or 0% LDH release. All samples were com pared against vehicle control. Experiments were per formed in triplicate.
Data was expressed as mean SD of triplicate experiments. Caspase 3 7 assay Caspase 3 7 activity was measured using Caspase Glo 3 7 assay kit from Promega, following the manufacturers in structions. Briefly, 10 103 cells were plated per well of the 96 well plate and treated as described in the LDH assay. Following treatment, Caspase Glo 3 7 reagent find more info was added and incubated for 30 min. at room temperature. The luminescence intensity was measured using lumin ometer. Cells treated with vehicle were considered as control against which treated cells were compared.
Additional Inhibitors,Modulators,Libraries blacken skin areas had been observed in T. orientalis extract handled group at 10 days, when compared with the handle or 1% minoxidil group. At 14 days, we observed that T. orientalis ex tract promoted hair growth additional prominently than both the control or 1% minoxidil group. At 17 days, dorsal skin hairs were entirely recovered in T. orientalis extract taken care of mice, whereas only 50% on the dorsal skin region during the manage group was covered with hairs. These outcomes propose that T. orientalis extract induces early telogen to anagen conversion of hair follicles. To determine no matter if T. orientalis extract induces hair development, we plucked thirty hairs from the dorsal skin center spot of every mouse at the two 14 and 21 days. Our results show that T. orientalis extract substantially stimu lated hair development, in comparison to the control group, and that the hair length of T.
orientalis extract handled mice cisplatin synthesis was drastically longer than that of your manage or 1% minoxidil treated group at 14 days. Effects of T. orientalis extract to the advancement and structure of mouse hair follicles An increase during the quantity and size of hair follicles is deemed as an indicator for that transition of hair growth from the telogen to anagen phases. To in vestigate the progression of hair follicles in the hair cycle, hematoxylin eosin staining was performed, considering that a rise during the dimension and amount of hair follicles is often observed in the deep subcutis during the anagen phase. Within the representative longitudinal sections, the number of hair follicles was improved in T. orientalis extract taken care of group, when compared with the manage group.
To quantify the hair selling effects, we performed the histomorphometric examination. Individual hair follicles had been classified following the Chases protocol. At day 7, the vast majority of sellekchem hair follicles in T. orientalis extract treated group progressed on the anagen phases II III, whereas the majority in manage group remained while in the telogen stage. At day 14, while the hair follicles of T. orientalis extract handled group were in anagen V VI, individuals of minoxidil taken care of and manage groups have been in anagen V and III, respectively. At day 21, the hair follicles in both T. orientalis extract and 1% minoxidil treated groups had been in anagen VI, whereas the control group remained in anagen V. These benefits sugest that topical application of T.
orientalis extract could induce an earlier anagen phase and prolong the mature anagen phase, in comparison to either the handle or 1% minoxidil handled group. Also, topical application of T. orientalis extract also significantly elevated the amount of hair follicles in mice, in comparison with the handle group at 7 and 14 days. At seven and 14 days, the amount of hair follicles in deep dermal regions of T. orientalis extract treated group was higher than that from the management group. Induction with the anagen phase by T. orientalis extract in telogenic C57BL 6 mice To elucidate the mechanism underlying the induction of anagen phases in T. orientalis extract treated group, we performed the immunohistochemistry analysis utilizing anti B catenin and anti sonic hedgehog antibodies.
Previously, it's been reported that both B catenin and Shh proteins are important to the development and maintenance of hairs not just in embryos, but also in adults. Several research also showed that B catenin and Shh induced the transition of the hair development cycle from your telogen to anagen phases and that transient activation of B catenin induced the anagen phase. Here, we demonstrate that the protein level of B catenin in T. orientalis extract treated group at 14 days was greater than that while in the manage or minoxidil treated group. Moreover, Shh is known for being expressed in inner root sheath and outer root sheath, sebaceous gland, hair follicles, and epidermis.
LNCaP and PC3 cells had been maintained in RPMI 1640 media supplemented Inhibitors,Modulators,Libraries with 10% fetal bovine serum beneath an ambiance of 5% CO2 at 37 C. Cells were harvested with the addition of 0. 25% trypsin with 0. 02% EDTA through the exponential growth phase. To the experimental therapies, CWR22Rv1 cells were cultured in RPMI 1640 media supplemented with 0. 05% fetal bovine serum containing Zyflamend or indi vidual herbal extracts reconstituted in dimethyl sulfoxide for cell proliferation assay, mRNA extraction and protein isolation. For inhibitor experiments, CWR22Rv1 cells have been pretreated with U0126 at a dose of 2 uM for thirty minutes and subsequently taken care of with Zyflamend for 24 hr. For experiments involving the common HDAC inhibitor TSA, TSA was extra to CWR22Rv1 cells at a concentration of 2 uM for 24 hours and compared to cells handled with Zyflamend.
In all experiments, 0. 1% DMSO was applied as the automobile handle. Cell proliferation The MTT assay was employed to assess relative cell development and viability, following the manufacturers instructions. Cells have been plated in 96 effectively plates within a volume of 100 ul culture medium. The culture medium contained various concen trations of Zyflamend or person herbal extracts. Cell proliferation neither was established at 0, 24, 48, 72, 96 hr post incubation. At every time level, a mixture of MTT,finish medium was extra and incubated at 37 C for 4 hr inside a CO2 incubator. Absorbance was measured on the SpectraCount microplate photometer.
BrdU incorporation assay Cells had been plated in 96 effectively plates and treated with various concentrations of Zyflamend for 48 hr and followed by a BrdU incorporation assay to assess relative DNA synthesis following the producers guidelines. After Zyflamend treatment, cells had been handled with BrdU for four hr as well as the BrdU incorporation was measured on the FluoroCount sellckchem microplate photometer at a 340 nm excitation and a 460 nm emission. Cellular and nuclear detection of p21 via immunofluorescent imaging CWR22Rv1 cells have been seeded on cover slips in RPMI 1640 media supplemented with 10% FBS below an atmos phere of 5% CO2 at 37 C overnight. Just before the treatment method, CWR22Rv1 cells had been maintained in RPMI 1640 media with 0. 5% FBS. For your observation of p21 and its nuclear localization, the cells have been pretreated with Zyflamend for 24 hr.
Soon after the therapy, the cells had been fixed using 2% paraformaldehyde for 15 min, followed by blocking with 10% goat serum for 1 hr, and anti p21 antibody overnight at four C. Immediately after washing with PBS, coverslips have been incubated with secondary antibody for a single hour at area temperature. Coverslips had been mounted on glass slides with Prolong Gold w DAPI Antifade reagent and analyzed by epifluorescence microscopy. 4 dual channel pictures have been captured from every sample employing a 60x goal lens. Picture evaluation was carried out employing NIS Aspects program v3. one. Mean fluorescence intensity per cell was calculated from the fol lowing, To assess p21 nuclear accumulation, p21 fluorescence was also measured inside discrete nuclear areas as defined working with a DAPI intensity threshold.
Down regulation of p21 by small interfering RNA CWR22Rv1 had been transfected with val idated p21 tiny interfering RNA or Stealth siRNA detrimental management employing Lipofectamine 2000 transfection re agent following the manufac turers instruction. 6 hr post transfection, cells were cultured with RPMI 1640 media containing 10% FBS over evening. Soon after recovery, media was replaced with 0. 05% FBS media containing automobile or Zyflamend for 24 hr at 37 C. The complete RNA was harvested for quantita tive true time polymerase chain reaction and cell quantity was established. Overexpression of p21 pRc CMV p21, containing total length wild sort p21 cDNA, was employed to overexpress p21. CWR22Rv1 cells have been plated overnight. pRc CMV p21 or pRc CMV was transfected employing Lipofectamine 2000 reagent in serum cost-free RPMI 1640 media.


text:
6th (Manawatu) Mounted Rifles
New Zealand Crown
New Zealand Army
New Zealand Mounted Rifles Brigade
Middle Eastern Theatre during the Great War
The 6th (Manawatu) Mounted Rifles was formed on March 17, 1911. They were mobilised during World War I as a squadron of the Wellington Mounted Rifle Regiment. They served in the Middle Eastern theatre of World War I and first saw action during the Battle of Gallipoli. As a part of the larger New Zealand Mounted Rifles Brigade (of the Anzac Mounted Division) they went on to serve in the Sinai and Palestine Campaign.[1][2]
Great War Battles
Battle of Gallipoli
Battle of Romani
Battle of Magdhaba
Battle of Rafa.
First Battle of Gaza
Second Battle of Gaza
Third Battle of Gaza
Battle of Megiddo (1918)
Between the Wars
It was remamed the 6th New Zealand Mounted Rifles (Manawatu) in 1921 and later to the simple, Manawatu Rifles ,which It was absorbed into 2nd Armoured Regiment on 29 March 1944.[1]
^ a b "firstaif". http://www.firstaif.info/pages/nz_mounted.htm.
^ "diggerhistory". http://www.diggerhistory.info/pages-nz/nzef.htm.
v · d · eNew Zealand Great War Mounted Regiments
1st Mounted Rifles (Canterbury Yeomanry Cavalry) • Queen Alexandra's 2nd (Wellington West Coast) Mounted Rifles • 3rd (Auckland) Mounted Rifles • 4th (Waikato) Mounted Rifles • 5th Mounted Rifles (Otago Hussars) • 6th (Manawatu) Mounted Rifles • 7th (Southland) Mounted Rifles • 8th (South Canterbury) Mounted Rifles • 9th (Wellington East Coast) Mounted Rifles • 10th (Nelson) Mounted Rifles • 11th (North Auckland) Mounted Rifles • 12th (Otago) Mounted Rifles • Auckland Mounted Rifle Regiment• Otago Mounted Rifle Regiment • Canterbury Mounted Rifle Regiment • Wellington Mounted Rifle Regiment
New Zealand in World War I
Cavalry regiments of New Zealand
Military units and formations of New Zealand in World War I
History of the Manawatu-Wanganui Region
1911 establishments in New Zealand
Jonathan Lund
The Spanish Gardener (film)
11th (North Auckland) Mounted Rifles — Active 1911 1921 Country New Zealand Allegiance New Zealand Crown Branch New Zealand Army Rol … Wikipedia
Queen Alexandra's Mounted Rifles — Cap badge of Queen Alexandra s Mounted Rifles Active 1864–present Country New Zealand … Wikipedia
Otago Mounted Rifle Regiment — Active 1911 1921 Country New Zealand Allegiance New Zealand Crown Branch New Zealand Army Role … Wikipedia
Wellington Mounted Rifle Regiment — Infobox Military Unit unit name=Wellington Mounted Regiment abreviation= caption= dates= 1911 1921 country= New Zealand allegiance= New Zealand Crown branch= New Zealand Army type= role= Mounted size= Regiment command structure= New Zealand… … Wikipedia
5th Royal Inniskilling Dragoon Guards — Infobox Military Unit unit name= 5th Royal Inniskilling Dragoon Guards caption= Regimental Badge dates= 1922 1993 country= United Kingdom branch= British Army type= Cavalry of the Line/Royal Armoured Corps role= Main Battle Tank… … Wikipedia
Battle of Chunuk Bair — Part of First World War Date 7–19 August 1915 Location … Wikipedia

redpajama_set_name: RedPajamaCommonCrawl


The FTC requires me to be transparent about any and all affiliate relations here on NotYourTypicalMommy.com. You the visitor should assume that any and all links in this website are affiliate links. If you decide to click the links found on NotYourTypicalMommy.com and visit a site recommended by me, a cookie may be saved in your browser that will enable me to receive compensation/commission on products or services that you purchase both now and in the future (of course, which are no extra cost to you).


In collaboration with the Houston Ballet Academy, Musiqa presents Anthony Brandt's chamber ballet, "Full Circle." Choreographer Oliver Halkowich creates a dance to Brandt's score for saxophone quartet, which spotlights the bluesy, brassy expressivity of the instruments. Further musical explorations include Robert Paterson's aptly-titled "Tongue and Groove" and David Ludwig's "Josquin Microludes," a work that transfigures Renaissance vocal motets. Works by James Primosch, Per Nørgård, and Zack Browning complete the program.


The introduction begins with an ethnographic anecdote of a man who walks away from a historical play being staged in the ruins of Firoz Shah Kotla and is stopped short by the invisible voice of a Muslim saint. This anecdote becomes the allegorical frame through which the book is introduced. The introduction sketches out the history of Delhi from the late 18th century to the contemporary period, but more importantly argues that for those who come to Firoz Shah Kotla, it is not the past as history that is important, but more importantly, the past as holding open potentialities for life, for the present and the future.
This chapter brings together oral history accounts, popular Urdu theological literature, and files from the Record Room of the ASI to bring together two parallel tracks; the growing presence of the jinn in post-Partition Delhi and the institutionalized amnesia of the official archives concerning everything prior to Partition and Independence in 1947. The chapter shows how the jinn are increasingly present in the blank spaces of the map, where the plans of the bureaucracy, the verdicts of the judiciary and the illegibility of the post-Partition Indian state attempt vast erasures of the city's Muslim landscapes, and how jinnealogy, the supersession of human chains of memory by the long lives of the jinn, challenges the magical amnesia of the state by allowing for other temporalities and modes of witnessing against the empty, homogenous time of the bureaucratic present.
Drawing upon ethnographic accounts from Firoz Shah Kotla as well as Urdu literary evocations of Delhi's ruin-scape, this chapter demonstrates how the experience of the sacred amidst these ruins is that of an immersion in multiple times simultaneously, and a cessation of time's "flow". The immersion in multiple times at ruins like Firoz Shah Kotla contains transformative potential for the people who come here. Here, the jinn-saints, often seen in visitors' dreams and visions wearing medieval robes, embody another time, different from the frenetic time of the contemporary city. This chapter shows how nineteenth-century colonial violence imbued the ruin known as Pir Ghaib (the invisible saint) with sacrality. This sacrality was linked to the nostalgic remembrance of the exiled Mughal emperor and the lost political order of Mughal rule, deeply tied to Sufi ethics and ideas of justice.
This chapter explores the ethics of nameless intimacy at Firoz Shah Kotla, where people who have known each other for twenty years or more seldom acknowledge caste and religious identity, rarely learning one another's proper names, referring to each other instead by nicknames and locational epithets. It shows how this ethics of namelessness points us towards a Sufi culture of gharib-navazi (hospitality to strangers) that is central to the healing power attributed to dargah spaces. The anonymity afforded by namelessness allows people to, even if temporarily, escape the often oppressive structures of social and familial identity. This estrangement, this making strange of the self, is the beginning of a process of reinventing one's self and relation to the world. One aspect of this process is expressing and acting on one's individual desires, even when they violate the normative morality of family and community.
This chapter discusses male-female interactions at Firoz Shah Kotla, where unrelated men and women often sit together for hours, speaking of desire, love, and loss. This is highly unusual in Delhi, a city with a reputation for violence against women. Here, in a space of Islam, a religion associated with a highly patriarchal order, women can be freer and more open, in both their interactions with men and in talking about their desires, than they can be in most public spaces in Delhi. Drawing on letters written by women at Firoz Shah Kotla and comparing them to women's voices as portrayed in pre-modern Rekhti poetry, it establishes a long tradition of intimacy with Muslim saintly figures that has allowed women to articulate individual longings and a sense of selfhood. It shows how the anti-patriarchal potentialities of Islam have continued to coexist along with the patriarchal juridical consensus.
This chapter begins with an ethnographic anecdote from Firoz Shah Kotla, where one of the author's friends equated Sat Yug (The Hindu idea of a past golden age) with contemporary Iraq. This was one of several moments in which he brought together and made equivalent things otherwise separated by vast temporal and conceptual distances. This chapter shows how we need to expand the idea of translations beyond language and texts to understand translation as a mode of being, which allows for the creation of shared sacred landscapes, ethical worlds and domains of meaning across conventional theological and communitarian divides. it discusses the historical processes of translation through which popular Islam has become an indistinguishable part of the ethical life of North India; the invisible religion which underlies the visible religious differences between Hinduism and Islam.
This chapter draws on eighteenth and nineteenth century accounts of prominent Sufi shrines in Delhi to show how these shrines were integrally connected to the ecology of the city. The author follows these accounts with his own visits to these sites in the contemporary city, where they have been completely disconnected from the ecological. Through oral histories, this chapter shows how this disconnect can be attributed to colonial policy, post-colonial growth, and pollution, all of which have radically changed the ontology of the sacred in the city from one of immanence, embedded in the local landscape, to one of immaterial transcendence. Only at a few sites like Firoz Shah Kotla, protected from development by archaeological policy, is an older memory and modality of the sacred still possible. The remembrance of older relations to the ecology includes the sanctification of the animals encountered in this space.
This chapter draws upon files from the post-colonial ASI, newspaper reports, and conversations with conservationists active in contemporary Delhi. It outlines a genealogy of conservation practice in Delhi and its changing relation to the life of the city, from the early twentieth century to the present. In the early twentieth century, for both British conservationists and Indo-Muslim antiquarians, these ruins were enchanted spaces, imaginative gateways to an otherwise irrecoverable Mughal past. The identification of Delhi's ruin-scape with Muslim sovereignty led to violent attacks on these sites during Partition violence, and this violence fundamentally changed the city's relation to its Muslim past. In the post-colonial era, Muslim monuments became spaces of darkness and death where no signs of (religious) life were permitted.
The author traces his autobiographical exploration of and investment in the ruin-scape of Delhi, and his concern that the forms of life indexed by these ruins seem impossible to recover for the majority of the contemporary city. This chapter looks at recent examples of jinn veneration from Lahore, an analysis of a recent Bombay film, and new conservation paradigms taking root in Delhi to think about how the rituals, ethics, and human and animal relations at Firoz Shah Kotla connect to wider trends in South Asia. The new conservation paradigms in Delhi wish to reconnect ruins to the human life of the city, and also to the city's ecology. While this new paradigm of preservation is secular, it shares remarkable similarities with the "religious" outlook of people at Firoz Shah Kotla.


Pinners is two great events in one. It's a conference featuring over 90 Pinterest-based classes taught by the best presenters in the nation. And it's also an expo with nearly 200 top businesses providing beautiful options in the worlds of DIY, crafts, cooking, self-improvment, photography, party planning, scrapbooking, holiday, beauty and fashion and all sorts of other great things. What's Pinners? It's where Pinterest comes to life. And it's your new favorite event!


text:
Home Politics APC Court Fixes April 7 For Suit Challenging Ajimobi As APC Dep. Chair...
Court Fixes April 7 For Suit Challenging Ajimobi As APC Dep. Chair South
Omidoyin, however, said the respondents had only been served March 23 in the court, adding that they required time to file their responses.
Benson Umoh
Sen. Abiola Ajimobi as Deputy National Chairman (South) of All Progressive Congress (APC) / Photo credit: Pulse
Justice Uchena Agomoh fixed the date to enable respondents file their responses to the amended originating summons brought before the court by Mr Taiwo Omidoyin, the plaintiff's Counsel.
The judge also said the the gap would allow the court to hear interlocutory applications filed by all parties in the suit.
"It is correct that there is a pending Motion on Notice before this court. The respondents were served this morning, which means the application is not ripe for hearing.
"I am mindful of the Appellant Court which has consistently said the Federal High Court and other courts must hear every application in cases.
"Therefore, allowing all applications and not hearing the new application is not fair.
"I will adjourn this matter to allow the application that has just been served to be responded to by all parties.
"This case is, therefore, adjourned until April 7 for parties to address the court on the hierarchical hearing order of the several applications'', Agomoh held.
Mr Michael Akinleye, the plaintiff and member of the APC in Ekiti, had approached the court with a suit bearing registration number FHC/AD/C8/8/2020 to challenge the propriety of the National Working Committee's appointment of Ajimobi.
Other listed as respondents are APC National Chairman, Adams Oshiomhole, National Working Committee, National Vice Chairman, South West, APC South West Zonal Caucus, Ekiti Chairman of APC, Ekiti Caucus of APC, and the APC.
Earlier, Omidoyin had informed the court of his pending application seeking amendment of the originating summon before the law.
He said an adjournment was needed to afford him the time to serve all the respondents in the matter.
According to Omidoyin, he is unable to serve respondents the motion of March 20 as the jurisdiction addresses of the respondents cannot be reached that same day.
Mr Anthony Adeniyi, Counsel for APC National Chairman, Adams Oshiomhole, and four others, told the court that he had a pending application raising objection on the suit fixed for hearing March 23.
Adeniyi, however, acknowledged the fact that he was served a motion on notice for amendment of the originating summons March 23 in court by the plaintiff's counsel.
Mr Akinwale Adeyeye, another respondents' counsel also attested to the receiving of the new application from Omidoyin which had yet to be responded to.
VIAPulse
Previous articleAjimobi Forms Oyo APC Reconciliation Team With Alao-Akala As Leader
Next articleAPC Leadership Tussle: Court Adjourns Untill March 31
Benson Umoh is a passionate writer who got his formal training in Mass Communication from the University of Nigeria Nsukka, as he has always wanted to write for a large audience. I'm very happy writing for you to read. Thanks for reading.


Jealous of Snow White's beauty, the wicked queen orders the murder of her innocent stepdaughter, but later discovers that Snow White is still alive and hiding in a cottage with seven friendly dwarfs. Disguising herself as a hag, the queen brings a poisoned apple to Snow White, who falls into a death-like sleep that can be broken only by a kiss from the prince.
Nicolet has implemented an online ticket system that enables patrons to reserve seats in advance, pay online on a secure site and print tickets at home. This will save patrons the extra trip to school to pay for and pick up tickets. Click Here to purchase tickets. If you have questions about our simple and secure process, please view our ticket purchasing help sheet (link to help sheet embedded in text).
Due to limited seating, and patrons acquiring tickets and then not attending the performance, ticket holders must arrive at least 10 minutes before show time to ensure their seats. At that time, unclaimed seats will be given to patrons on a waiting list who could not acquire tickets. Thank you!


This feeder sits on top of the inner cover with the center trunk over the hole in the inner cover. An inverted vented cup sits over the truck then the plastic cover goes over the feeder. This feeder minimizes bees drowning. It needs to be used with a spare hive box and an outer cover.


Last summer (the summer of 2017), Liz and I drove from Ontario down through the Eastern United States on our way to a conference I was attending in Orlando, Florida. We figured it was the perfect opportunity to explore as many breweries and fun beer bars as we could along the way. And as a bonus, several of the stops along the way included haunted locales.
Such as the afternoon of July 25th when we were in Savannah, GA and popped into Moon River Brewing Company. Admittedly, I wasn't in the finest of forms – I had been recovering from a nasty bout of food poisoning from the night before (that will definitely be the last time I eat raw oysters) and that combined with the heat and humidity of Savannah at the end of July was slowing me down quite a bit.
That's part of where the "Blue Me" comes from.
A second part of the blue comes from the fact that we didn't get a chance to talk to any of the staff there about any supernatural experiences. The bar, where we normally like to sit because that way you can get to talk to the bartender(s), was packed and busy. We ended up with a table and our server didn't have any fun ghostly stories to share. Oh well, we always take our chances.
A Savannah Morning News article references staff members experiencing beer bottles being thrown at them as well as odd and eerie shadows walking by.
The billiards room is alleged to have a ghost that goes by the name of Toby. "Toby is known to breeze past you or even push you out of the way," Christopher Lewis said in the Savannah Morning News article. "We don't know why he's here, but he likes to play tricks on people." And on the third floor several people have spotted an elusive lady in white. She was even captured on video by a team of ghost hunters from the SyFy Channel.
On the Moon River website, there's a video from TAPS that shows an eerie fog and orbs floating through an empty room. It also shows a chilling short shadow figure moving about behind a pool table. The short video above (complete with X-Files theme music) shows the eerie sights.
There is, of course, a much longer video on the Daily Motion website featuring Ghost Adventures' visit and overnight stay at Moon River Brewing Company.
We didn't see or hear about anything supernatural that afternoon.
Because I was on a mostly water diet, I ended up doing something I rarely do. I only had a single beer there: the Wild Wacky Wit WItbier. It was light, citrusy and fruity. Just what the doctor ordered as a relief from the heat and from my tummy issues.
And that leads to the third reason I was a bit of a blue moon. I'm also a little blue that I didn't get a chance to try their Apparition Ale, which was no longer in production at the time we visited. I mean, this ad alone makes me want to help make it disappear.
Hmm, does that mean we need to keep an eye out for it so we can return for another visit?
I'm up for that. Perhaps then you won't see me standing alone, without a dream in my heart, without a pale ale of my own.


Do you want more information about our oils? Have you a specific question about buying or distributing our products? Contact us!
Call us directly or complete the contact form! We will get back to you as quickly as possible!
Follow our news and innovations on social networks!


We helped build a yurt for one of our members that we were able to borrow for All Hallows Fantasy Faire.
We cut the roof beams, cut & glued fabric to the tarp and built it in about 2 days.
The 16′ diameter yurt was borrowed and featured at All Hallows Fantasy Faire in Sonora, CA.
Now this yurt is on the property of one our members as his workshop. It is heavy duty to withstand the elements including snow.
We are working on raising funds to build our own that is lighter material and using twine instead of bolts to attach the lattice work to be more period accurate & more historically correct.
Check out our Fairy House Yurt Campaign on GoFundMe!


text:
SpaceX Breaks Ground on Launch Pad for Huge Private Rocket
By Space.com Staff published 13 July 11
Illustration of SpaceX's Falcon Heavy rocket, which the company says will be the most capable rocket operating today. (Image credit: SpaceX)
The company Space Exploration Technologies, better known as SpaceX, broke ground Wednesday (July 13) on a new launch site for its massive heavy-lift rocket.
SpaceX is upgrading a site called Space Launch Complex 4-East at Vandenberg Air Force Base near Santa Barbara, Calif. The refurbished launch complex will host SpaceX's Falcon Heavy rocket, which the company says will be more powerful than any launch vehicle currently in service.
Several dignitaries attended Wednesday's event, including SpaceX CEO and chief rocket designer Elon Musk and California Lt. Gov. Gavin Newsom, company officials said.
According to the Los Angeles Times, SpaceX is pouring about $30 million into the new launch site, with the aim of securing large launch contracts with the Air Force. Currently, the United Launch Alliance — a joint venture of Boeing and Lockheed Martin — launches all payloads for the United States Department of Defense.
Musk made it clear that SpaceX wants a piece of the military satellite market.
"These are difficult fiscal times for our federal government, and the Falcon vehicles can save the Department of Defense almost $2 billion per year in launch costs, while increasing reliability and capability," Musk said in a statement. "This presents a great opportunity for the DoD to avoid cancelling other programs and minimize reductions in personnel as budgets contract."
The Falcon Heavy boasts 27 of SpaceX's Merlin engines, three times as many as the company's Falcon 9 rocket. The heavy-lifter will be able to loft about 117,000 pounds (53,000 kilograms) of cargo to orbit, about twice as much as the United Launch Alliance's Delta 4, which is the most powerful rocket currently in operation, company officials said.
The Falcon Heavy will be the most powerful rocket since NASA's Saturn V, which blasted the Apollo spacecraft toward the moon. [Video: Falcon Heavy: Most Lift Since Saturn V]
The new heavy-lifter is to arrive at Vandenberg by the end of 2012, and its inaugural flight will follow soon after, SpaceX officials said.
The Falcon Heavy is designed to stand 227 feet (69.2 meters) tall and weigh about 3.1 million pounds (1.4 million kg). It would incorporate the standard Falcon 9 rocket, with two liquid-powered Falcon 9 first-stage boosters strapped on its sides.
The price for a launch aboard the new Falcon Heavy is set for $100 million, compared with the $50 million price tag for a Falcon 9 liftoff, Musk has said.
Space Launch Complex 4-East has a long history, but it hasn't been used for more than half a decade. The first launch from the site took place in 1964, while the last blasted off in 2005.
Follow SPACE.com for the latest in space science and exploration news on Twitter @Spacedotcom and on Facebook.
Space.com Staff
News and editorial team
Space.com is the premier source of space exploration, innovation and astronomy news, chronicling (and celebrating) humanity's ongoing expansion across the final frontier. Originally founded in 1999, Space.com is, and always has been, the passion of writers and editors who are space fans and also trained journalists. Our current news team consists of Editor-in-Chief Tariq Malik; Editor Hanneke Weitering, Senior Space Writer Mike Wall; Senior Writer Meghan Bartels; Senior Writer Chelsea Gohd, Senior Writer Tereza Pultarova and Staff Writer Alexander Cox, focusing on e-commerce. Senior Producer Steve Spaleta oversees our space videos, with Diana Whitcroft as our Social Media Editor.
Artemis 1: Going back to the moon
Virgin Orbit sends 7 satellites to orbit in fourth mid-air launch
Halley's Comet: Facts about history's most famous comet
Virgin Orbit launching 7 satellites today: Watch it live
China's Shenzhou 13 astronauts manually fly cargo ship for emergency docking test


You can buy Siskiyou SportsSFB100 NFL Pewter Belt Buckle- New York Jets at 1 store. At UnbeatableSale.com the price is only $21.00 for Siskiyou SportsSFB100 NFL Pewter Belt Buckle- New York Jets.
Uncle ordered two nfl clothing from this retailer. They were very good deals and were very quickly delivered to us even with standard shipping. We are very happy with this transaction.


text:
Q: English complement I have this sentence below:

I can picture him now, sitting at his desk pilled high with manuscripts and reference books, leaning back in his chair and looking out of the window while a cigarette burned between his fingers.

The question is for the part of the sentence from 'sitting ...'. What is this structure? Is it a complement of the sentence? If the answer is positive, could you guys please link me to the document/documents for references?

redpajama_set_name: RedPajamaStackExchange


Sanjay Puri is a global entrepreneur whose powerful connectivity in government and business places him at the forefront of thought leadership and advisement as he challenges existing industries with disruptive innovations.
Puri is the founder and CEO of Wellisen, Asia's break-through nutraceuticals and personal care company committed to ameliorating world hunger and advancing health and wellness across the globe. Wellisen has advisory alliances and research partnerships in the U.S. and India, product-based technologies, certified product offerings, internal sourcing and production, as well as an internal research and development (R&D) team that is among the best in the business. Wellisen's diverse portfolio includes Spirulina, a protein-rich superfood considered one of the most nutrient-rich foods on Earth. Phycocyanin, a natural blue pigment derived from Spirulina with numerous health-promoting benefits, is one of the core ingredients that Wellisen manufactures through its proprietary extraction process at its state-of-the-art facility in Mysuru, India. Wellisen's line includes other algal products, emerging applications and new formulations in process. Wellisen also offers customized formulations and proprietary solutions, and partners, invests or incubates with consumer product companies that share its high standards. Wellisen's cosmetics make up Asia's most up and coming luxury line. Wellisen has U.S. offices in Reston, Virginia near Washington, DC.
As a highly sought-after media contributor, Puri has been published and quoted in Forbes, The New York Times, The Washington Times, The Hindu, Inc. 42, The Afternoon DC, Indian Express, Hindustan Times, The Times of India, etc. He has hosted numerous conferences and roundtable discussions in cooperation with the U.S. Senate and U.S. House of Representatives, and moderated Q&A sessions on Capitol Hill. He has testified before the U.S. House Committee on Foreign Affairs as an expert witness on U.S.-India relations, trade and energy. He has presented at the Wharton India Forum, Harvard Business School, National War College, Heritage Foundation, International Leadership Foundation, United States Institute of Peace, Duke India Business Forum, Kellogg School of Management, etc. He has been featured on CNN, BBC and National Public Radio (NPR). Puri organized key political and corporate meetings during Prime Minister Narendra Modi's visit to the U.S., and continues to lead business delegations to India. He actively engages public officials at federal, state and local levels of government.
Puri founded Optimos Inc., a strategy and enterprise information technology company that was sold in 2014. He started his entrepreneurial journey in 1994 through a grant from the National Institute of Health (NIH) where he led a team that used artificial intelligence techniques to diagnose patients with Alzheimer's. His clients have included the Library of Congress, Federal Reserve Bank, U.S. Mint, and the National Labor Relations Board. Puri is also the founder of Autonebula, a connected vehicle incubator/accelerator; and ACEL360, a multi-module accelerator for small and medium sized businesses. Puri founded the Alliance for U.S.-India Business (AUSIB) and the United States India Political Action Committee (USINPAC) to leverage the smart power of the more than 3 million strong Indian-American community, and to bring about outcomes that matter.
In 2004, Puri was named "Entrepreneur of the Year" by the Virginia Minority Supplier. He received a Lifetime Achievement Award from DY Patil University. He holds an MBA in Finance and Investments from George Washington University. Puri has been recognized in the U.S. Congressional Record for his achievements.


text:
laos - General & Misc
Ministry orders Registration of Social Media news Platforms
Authorities have told the administrators of social media news platforms to register their accounts or pages, or they could face legal action.
Date: 15 July 2019; Type: Legal News; Source: Vientiane Times
Government seeks to minimize Budget Burdens and Debt Distress
The government has outlined measures to boost national revenue for 2020 while ensuring the effectiveness of state investment projects.
Ministry urges Taxpayers to limit Cash Payments
Taxpayers are being encouraged to pay their dues electronically via commercial banks as part of its efforts to plug financial loopholes, the Ministry of Finance has announced.
NA approves New Law to safeguard Local Products from Unfair Imports
The National Assembly (NA) on Friday approved a new law relating to anti-dumping and countervailing duties in order to protect domestic products from unfair imports.
Date: 17 June 2019; Type: Legal News; Source: Vientiane Times
Justice Ministry to upgrade Sentencing Enforcement Officials
The Ministry of Justice plans to train and supply more legal specialists to assist local authorities in the enforcement of court sentences, according to a senior ministry official.
New Service Charter planned to ease Cross-Border Trade
The Ministry of Industry and Commerce plans to introduce a new service charter to reduce the time it takes to authorise imports and exports, as part of efforts to improve the business climate.
Date: 21 May 2019; Type: Legal News; Source: Vientiane Times
Telecom Regulator proposes Online Sim Card Registration
The Lao Telecommunication Regulatory Authority is developing an application for use as a possible option to speed up sim card registration.
Date: 15 May 2019; Type: Industry Updates; Source: Vientiane Times
Government slaps Heavy Fines on Tobacco Rule Violators
Producers and importers of tobacco products will be penalised with large fines and additional measures if the packages that contain their products do not carry the required instructions and health warnings, according to a new regulation.
Date: 03 March 2019; Type: Legal News; Source: Vientiane Times
State Agencies urged to comply with Procurement Law
State agencies that intend to spend more than 5 million kip of state funds must put project contracts up for bid, according to the Ministry of Finance.
Date: 26 February 2019; Type: Legal News; Source: Vientiane Times
Laos, Japan celebrate 20 Years of Legal, Judicial Cooperation
The Lao and Japanese governments have highlighted 20 years of achievement in cooperation to strengthen the legal and judicial sectors in Laos, especially with regard to human resource development.
Date: 19 February 2019; Type: Industry Updates; Source: Vientiane Times
Round Table Retreat reviews Implementation of 2018 Recommendations
The government and development partners met in Vientiane on Friday to discuss translation of the key recommendations of the 2018 Round Table Meeting into concrete follow-up actions.
Government unveils New Development Vision
The government has unveiled a new national development vision, which it plans to achieve from 2021 to 2025.
Date: 31 January 2019; Type: Legal News; Source: Vientiane Times
VP urges Justice Officials to ensure Law Enforcement
Vice President Phankham Viphavanh yesterday advised the justice sector to strictly implement the country's laws in a bid to ensure justice for all members of society.
Date: 29 January 2019; Type: Industry Updates; Source: Vientiane Times
Ministry moves Forward with Justice Development Plan
The Ministry of Justice will carry out plans to process outstanding court cases, raise public awareness of improvements in the law, conduct capacity building for officials, and resolve unsettled legal issues.
Government announces List of Controlled Businesses
Prime Minister Thongloun Sisoulith has approved a list of businesses that are subject to government control, while also stating that types of business not on the list would find it easier to operate.
Laos, Luxembourg review Rule of Law and Legal Education Programmes
Government officials met with representatives of the Luxembourg government in Vientiane yesterday to review the progress of the LAO-031-Legal Teaching and Training and Promotion of the Rule of Law Concept project and make plans for the year ahead.
Date: 18 December 2018; Type: Industry Updates; Source: Vientiane Times
Nation celebrates Universal Declaration of Human Rights
Government agencies and diplomatic and international organisation representatives gathered in Vientiane to mark the 70th anniversary of the Universal Declaration of Human Rights and its lasting impact, which was supported by the United Nations, European Union and Australia.
New Law to better serve Lao Diplomatic Mission Overseas
Members of the National Assembly (NA) yesterday passed a newly drafted law to better facilitate Laos' diplomatic mission in foreign countries and to protect the interests of the nation and Lao expats overseas.
Date: 11 December 2018; Type: Legal News; Source: Vientiane Times
NA members ask Court, Prosecutor to strive harder for Justice
Members of the National Assembly (NA) have called for the People's Supreme Prosecutor and People's Supreme Court to work harder to ensure full justice in society.
NA approves Government reshuffle
The National Assembly (NA) yesterday approved a reshuffle and appointments in public security, national defence and the banking sectors following the proposals of Prime Minister Thongloun Sisoulith.
Go Online to partner with 8 Million MSMEs
Jakarta, Jul 1 (ANTARA) - The Communications and Information Ministry is targeting eight million micro, small and medium enterprises (MSMEs) to be partners of the Go Online program. Involving MSMEs and marketplace in the country, Go Online is a collaborative program between the government, MSMEs, and online shopping platforms to level digital market access and build a digital economy in Indonesia.
CUEPAC urges Government to review Minimum Wage
KUALA TERENGGANU, Jun 30 (Bernama) - The Congress of Unions of Employees in the Public and Civil Services (CUEPACS) has urged the government to review the minimum wage in an effort to reduce the people's cost of living.
MITI calls SMEs to sign up for IR4.0 Assessment
GEORGE TOWN, Jun 28 (Bernama) - The Ministry of International Trade and Industry (MITI) is encouraging small and medium-sized enterprises (SMEs) in the manufacturing sector to sign up for the National Policy on Industry 4.0 Readiness Assessment (Industry4WRD-RA).


I hope you are doing well. I just wanted to check in and let you guys know I finally got around to publishing a short story I've been working on. Fragments is a horror story about a wife and mom struggling with CFS, depression, and ghostly encounters after a serious accident.
Fragments centers around out-of-body, near-death experiences and my own twist on things. This story is a prequel to something I'm working on or part one of three–depending on how long the next stories end up being. I figured it was perfect for Halloween, so I went ahead and put some time into making it ready.
Grab your digital copy for only 99 cents on Amazon here, or I've also enrolled it in Kindle Unlimited, so go check it out and leave a review!
Jessica Fisette is the author of The Vanquished, the first book in The Soul Reaper series, and Fragments, a short story. Her hobbies include discovering the benefits of natural medicine, wine tasting, and trying new recipes in the kitchen. She likes to unwind by typing out a scene or two in her latest obsession or indulging in a good book. Having been passionate about writing since she was a little girl, she is constantly coming up with new ideas for future stories and creating unique, strongwilled–albeit flawed–characters to overcome the difficult obstacles she places before them.


text:
"I'm Alive" is a song written by Tommy James and first recorded and released in 1968 by American singer Johnny Thunder.

Thunder's recording of "I'm Alive" was a "raucous" rock single, featuring "Verbal Expressions of T.V." as its B-side. Bob Dylan, who had heard "I'm Alive" on the radio, was asked by Rolling Stones Jann Wenner in 1969 if he was impressed by anything in the rock music scene and pointed to the song: "Never heard it either, huh? Well, I can't believe it. Everyone I've talked to, I've asked them if they've heard that record. It was one of the most powerful records I've ever heard. It's called 'I'm Alive.' By Johnny Thunder. Well, it was that sentiment, truly expressed. That's the most I can say ... if you heard the record, you'd know what I mean."

James recorded and released his version in 1969 with his band the Shondells as part of their album Crimson & Clover. It was released as a B-side for the single "Crystal Blue Persuasion".

Thunders' version was later used by Samsung in their advertisement of the Galaxy S6 Edge in 2015. That version was also used in the soundtrack for the 2018 film American Animals.

Don Fardon version

English singer Don Fardon released a cover as a single also in 1969. His recording has been featured in a UK television advertisement for Five Alive fruit drinks, and a Dutch Vodafone commercial.

On the back of the success of the latter, "I'm Alive" was reissued in the Netherlands and in March 2011 it reached the Top 20 of the Dutch singles chart. Don Fardon's version has been remixed and released as a single by English DJ Ashley Beedle.

Other versions
In 1975, Swedish band Blue Swede recorded a mash-up which combined verses of I'm Alive with others borrowed from Hush, by Deep Purple.  The song was also recorded by Tom Jones. The track appeared on his 2008 album 24 Hours.

Canadian garage-punk band, UIC, from Toronto, Ontario, recorded their version of I'm Alive on their 1986 album Our Garage.

References

1969 songs
Songs written by Tommy James

redpajama_set_name: RedPajamaWikipedia


text:
Home/12"/WHY BOTHER? – A YEAR OF MUTATIONS LP
WHY BOTHER? – A YEAR OF MUTATIONS LP
"In the heart of the heartland, a mere ten miles from where the airplane carrying Buddy Holly crashed into a frozen cornfield, lies Mason City, Iowa. A town so small that it's pre-pandemic punk scene (with 20+ bands) went almost entirely unnoticed. That is, until the music of WHY BOTHER? and the "Punk on the Plains" compilations (curated by WB? drummer Paul), were made available by Tremendo Garaje – one of YouTube's most obsessive gateways into contemporary underground punk music. WHY BOTHER? is Terry on vocals + synth, Speck on guitar, Pamela on bass, and Paul on drums. Their sound takes the classic Midwestern punk approach of The Zero Boys and Toxic Reasons, adding in warm layers of synth and sci-fi thematics ala The Spits and darker post-punk moments in the vein of early Joy Division. Yet there's so much more to this band that defies comparison, for WHY BOTHER? are truly a singular experience. "A Year of Mutations" collects tracks from the first four WHY BOTHER? EPs, all of which were released in limited cassette and 7″ lathe cut runs in 2020 and 2021. These have all been remastered, paired with several new tracks exclusive to this release, and repackaged in a fresh layout with artwork from D.H. Strother. WHY BOTHER? have no plans or intentions to perform live, but their basement 8-track recording setup shows no signs of slowing down. Expect more in 2022 & beyond!"
NO DEFENCES – RELEASED LP
SAUNA YOUTH – DEATHS LP
STRAIGHTJACKET NATION – S/T LP
VANILLA POPPERS – S/T LP


Some people start testing the musical waters with social media, posting song snippets against a back beat from YouTube. Once in a while it's surprisingly natural and seems destined to develop into something true.
When you look back to hearing that sax player on TV for the first time, how did it make you feel?
The day I first saw a sax player on TV it gave me a feeling I've never had before; a wonderful feeling. It sparked something inside of me.
When did you start playing in school?
I started playing at the age of 11, when I was in the sixth grade.
What type of sax do you own and why?
I own an alto, but I am versatile and can play tenor and soprano as well.
What is the most rewarding part of playing alto sax?
I can play really nice clean high notes closer to a soprano, and I can also play low notes closer to a tenor, which gives me a bit more flexibility to please the ear of the audience.
After playing so many gigs on a reed that you really like and don't want to change, you start clipping just out of stubbornness of not wanting to change it!
Where do you go, in your head, when you hear or play smooth jazz?
I go into a whole different atmosphere, I think of nature and peace. A deep meditation mode. I think of past events and where the future will take me.
What inspires you when you compose?
To know that I'm putting something together that comes from within me and that what I feel people can connect to.
What about all the awards and competitions you've won in school?
It meant to me that I am capable and that I have the potential to pursue and be successful in my career as a sax player.
Who are the musicians you record with and what do they play?
I have yet to record with anyone but am open to it in the future. All of my work so far has been myself as a solo artist along with a beat that's been created.
What is the most important thing to you in collaborating in an ensemble?
Articulation and tightness, for everyone to feel connected, vibes must be on point. With all due respect, they all need to be up to par, on skill and sound honest.
Who would you like the chance to perform with?
There are many in mind, including Pieces of a Dream, Brian Culbertson, Paul Hardcastle and Jeff Lorber.
It's early in your career. Where do you see yourself going in the immediate future?
Literally around the world, hoping to inspire as many people as I can.
What clubs have you played?
I have played at various places in Arkansas, Tennessee, Missouri and in Texas.
Where would you most like to play?
I have yet to play on a cruise. To say the least, that's one to mark down in the books for the future.
What are the names of some of your singles?
I currently do not have any singles but I am working on some upcoming singles and then an album.
Where is your recording studio?
Do you play full-time? If not, how will you make a path to get you there?
I do not currently play full-time but I would like to make it my full-time career. I am at work on my plan of action to get there.
Who are your biggest influencers – personal and musically speaking?
From Charlie Parker, John Coltrane, and Miles Davis all the way to Kenny G, Gerald Albright, Dave Koz and David Sanborn. But there are so many more I could name.
What do you want people to know about you (your music) that they don't yet know?
I want them to feel what I feel, the happiness and passion and love that I have for music, to be spread among people and lift their spirits.
Are you the "one to watch"?
With confidence I'm going to say that I definitely am, that when the energy is right and the music is flowing you will probably catch me moving around or dancing. If the energy and vibe are right good things can definitely come from that. Either way the music will speak for itself.
For more information visit https://www.facebook.com/nelson.pettigrew.
Photos courtesy of and with permission of Nelson Pettigrew.


text:
Das Gut Obergedern ist ein Bauernhof und ländliches Anwesen mit kleiner privater Parkanlage im Südosten der Stadt Witten in Nordrhein-Westfalen. Es befindet sich am Rand der Ruhraue im Ortsteil Gedern (Stadtteil und Gemarkung Annen) und liegt am Westhang des Arenbergs, eines bewaldeten Höhenrückens im Ardeygebirge.

Geschichte
Als Lehnsgut der Herren von Burg Volmarstein wurde der Hof 1351 an deren Lehnsmann Dietrich III. von Mallinckrodt übertragen. Der Hof hieß Middeldorpshof, später nach seinem Besitzer Pampus und Rüping und blieb gegenüber dem benachbarten Haus Mallinckrodt abgabepflichtig.

Ab 1950 gehörte der Hof der Familie Zimmermann.
2006 erwarb ein Bioland-Betrieb die Hofstelle und bewirtschaftet sie seither.

Literatur

Weblinks
 Kornkammer Haus Holte

Einzelnachweise

Obergedern
Obergedern
Gehöft in Nordrhein-Westfalen
Baugruppe (Städtebau) in Europa


text:
from __future__ import unicode_literals

from django.contrib import admin

from account.models import Account, SignupCode, AccountDeletion

class SignupCodeAdmin(admin.ModelAdmin):

    list_display = ["code", "max_uses", "use_count", "expiry", "created"]
    search_fields = ["code", "email"]
    list_filter = ["created"]

admin.site.register(Account)
admin.site.register(SignupCode, SignupCodeAdmin)
admin.site.register(AccountDeletion, list_display=["email", "date_requested", "date_expunged"])

redpajama_set_name: RedPajamaGithub