text:
Vujčić, Zoran
http://cherry.chem.bg.ac.rs/APP/faces/author.xhtml?author_id=orcid%3A%3A0000-0002-8963-2439&item_offset=0&project_offset=0&sort_by=dc.date.issued
Vujčić, Zoran (113)
Production, purification and characterization of enzymes and small molecules and their application as soluble or immobilized in food biotechnology, biofuels production and environmental protection Interakcije prirodnih proizvoda i njihovih analoga sa proteinima i nukleinskim kiselinama
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200168 (University of Belgrade, Faculty of Chemistry) Spanish MICINN [CTQ2011-28398-CO2-01]
FEBS Collaborative Experimental Scholarship, Oslo, Norway Mechanistic studies of the reactions of transition metal ion complexes with biologically relevant molecules
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200026 (University of Belgrade, Institute of Chemistry, Technology and Metallurgy - IChTM) project ApliMetaFarma [RC.2.2.08-0046]
STSM Grant from COST Action [BM1403] ICGEB [CRP/YUG11-02]
ICGEB research project [CRP/YUG11-02] The synthesis of aminoquinoline-based antimalarials and botulinum neurotoxin A inhibitors
Development of molecules with antiinflammatory and cardioprotective activity: structural modifications, modelling, physicochemical characterization and formulation investigations Biotechnology in vitro - crop, medicinal and endangered plant species
Microbial diversity study and characterization of beneficial environmental microorganisms Nanostructured Functional and Composite Materials in Catalytic and Sorption Processes
Unapređenje tehnologije konvencionalnog sušenja drveta sa aspekta kvaliteta i utroška energije The Impact of Mining Wastes from RTB Bor on the Pollution of Surrounding Water Systems with the Proposal of Measures and Procedures for Reduction the Harmful Effects on the Environment
International Centre for Genetic Engineering and Biotechnology (ICGEB) [CRP/YUG11-02] Joint Serbian-Spanish Action
Joint Serbian-Spanish Action [A IB2010 SE-00122] Spanish MICINN [CTQ2008-00578]
Austrian Science Fund (FWF) Project Number P 25613 B20 to ISD Deutscher Akademischer Austausch Dienst (DAAD)
Federation of European Biochemical Societies ICGEB research [CRP/YUG11-02]
ICGEB Research Grant [CRP/YUG11-02] Study of structure-function relationships in the plant cell wall and modifications of the wall structure by enzyme engineering
Interactive role of dyslipidemia, oxidative stress and inflammation in atherosclerosis and other diseases: genetic and biochemical markers Antioxidative defense, differentiation and regeneration potential of tissue specific mesenchymal stem cells during ageing
Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale
Margetić, Aleksandra; Stojanović, Sanja; Ristović, Marina; Vujčić, Zoran; Dojnov, Biljana
AU  - Margetić, Aleksandra
AU  - Stojanović, Sanja
AU  - Ristović, Marina
AU  - Vujčić, Zoran
AU  - Dojnov, Biljana
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4801
AB  - There is an urgent need to increase the daily intake of insoluble dietary fiber, and at the same time to find new sources and new production technologies. We hypothesized that fungal enzymes directly involved in lignocellulosic material hydrolysis (Aspergillus and Trichoderma enzyme cocktails) will change the fiber structure particularly efficiently after the action of laccase (Trametes versicolor enzyme cocktail). Enzymes production on an inducing substrate (same as starting material for obtainment of insoluble dietary fibers) and their usage resulted in obtainment of novel insoluble dietary fibers with better characteristics, 24% higher swelling, 43% higher WRC and 57% higher ORC compared to insoluble dietary fibers from triticale (already proven to be a good food additive). Changes in structure were analyzed by FTIR and microscopic analysis. Antioxidative performance of the obtained products, new insoluble and released soluble dietary fibers, was analyzed in detail. Newly obtained soluble dietary fibers demonstrated up to 20 times higher antioxidant activity compared to untreated fibers (ABTS and DPPH tests). These results suggest their good performance as a future food additive. At the same time, they prove the hypothesis that the use of enzyme cocktails rich in laccase is a good choice for biological pretreatment in this process.
T2  - LWT - Food Science and Technology
T1  - Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale
DO  - 10.1016/j.lwt.2021.111291
author = "Margetić, Aleksandra and Stojanović, Sanja and Ristović, Marina and Vujčić, Zoran and Dojnov, Biljana",
abstract = "There is an urgent need to increase the daily intake of insoluble dietary fiber, and at the same time to find new sources and new production technologies. We hypothesized that fungal enzymes directly involved in lignocellulosic material hydrolysis (Aspergillus and Trichoderma enzyme cocktails) will change the fiber structure particularly efficiently after the action of laccase (Trametes versicolor enzyme cocktail). Enzymes production on an inducing substrate (same as starting material for obtainment of insoluble dietary fibers) and their usage resulted in obtainment of novel insoluble dietary fibers with better characteristics, 24% higher swelling, 43% higher WRC and 57% higher ORC compared to insoluble dietary fibers from triticale (already proven to be a good food additive). Changes in structure were analyzed by FTIR and microscopic analysis. Antioxidative performance of the obtained products, new insoluble and released soluble dietary fibers, was analyzed in detail. Newly obtained soluble dietary fibers demonstrated up to 20 times higher antioxidant activity compared to untreated fibers (ABTS and DPPH tests). These results suggest their good performance as a future food additive. At the same time, they prove the hypothesis that the use of enzyme cocktails rich in laccase is a good choice for biological pretreatment in this process.",
journal = "LWT - Food Science and Technology",
title = "Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale",
doi = "10.1016/j.lwt.2021.111291"
Margetić, A., Stojanović, S., Ristović, M., Vujčić, Z.,& Dojnov, B.. (2021). Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale. in LWT - Food Science and Technology
Elsevier., 145, 111291.
https://doi.org/10.1016/j.lwt.2021.111291
Margetić A, Stojanović S, Ristović M, Vujčić Z, Dojnov B. Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale. in LWT - Food Science and Technology. 2021;145:111291.
doi:10.1016/j.lwt.2021.111291 .
Margetić, Aleksandra, Stojanović, Sanja, Ristović, Marina, Vujčić, Zoran, Dojnov, Biljana, "Fungal oxidative and hydrolyzing enzymes as designers in the biological production of dietary fibers from triticale" in LWT - Food Science and Technology, 145 (2021):111291,
https://doi.org/10.1016/j.lwt.2021.111291 . .
Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3
Radulović, Olga; Stanković, Slaviša; Stanojević, Olja; Vujčić, Zoran; Dojnov, Biljana; Trifunović-Momčilov, Milana; Marković, Marija
(MDPI, 2021)
AU  - Radulović, Olga
AU  - Stanković, Slaviša
AU  - Stanojević, Olja
AU  - Trifunović-Momčilov, Milana
AU  - Marković, Marija
AB  - Duckweed (L. minor) is a cosmopolitan aquatic plant of simplified morphology and rapid vegetative reproduction. In this study, an H. paralvei bacterial strain and its influence on the antioxidative response of the duckweeds to phenol, a recalcitrant environmental pollutant, were investigated. Sterile duckweed cultures were inoculated with H. paralvei in vitro and cultivated in the presence or absence of phenol (500 mg L−1), in order to investigate bacterial effects on plant oxidative stress during 5 days. Total soluble proteins, guaiacol peroxidase expression, concentration of hydrogen peroxide and malondialdehyde as well as the total ascorbic acid of the plants were monitored. Moreover, bacterial production of indole-3-acetic acid (IAA) was measured in order to investigate H. paralvei's influence on plant growth. In general, the addition of phenol elevated all biochemical parameters in L. minor except AsA and total soluble proteins. Phenol as well as bacteria influenced the expression of guaiacol peroxidase. Different isoforms were associated with phenol compared to isoforms expressed in phenol-free medium. Considering that duckweeds showed increased antioxidative parameters in the presence of phenol, it can be assumed that the measured parameters might be involved in the plant's defense system. H. paralvei is an IAA producer and its presence in the rhizosphere of duckweeds decreased the oxidative stress of the plants, which can be taken as evidence that this bacterial strain acts protectively on the plants during phenol exposure.
PB  - MDPI
T2  - Antioxidants
T1  - Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3
DO  - 10.3390/antiox10111719
author = "Radulović, Olga and Stanković, Slaviša and Stanojević, Olja and Vujčić, Zoran and Dojnov, Biljana and Trifunović-Momčilov, Milana and Marković, Marija",
abstract = "Duckweed (L. minor) is a cosmopolitan aquatic plant of simplified morphology and rapid vegetative reproduction. In this study, an H. paralvei bacterial strain and its influence on the antioxidative response of the duckweeds to phenol, a recalcitrant environmental pollutant, were investigated. Sterile duckweed cultures were inoculated with H. paralvei in vitro and cultivated in the presence or absence of phenol (500 mg L−1), in order to investigate bacterial effects on plant oxidative stress during 5 days. Total soluble proteins, guaiacol peroxidase expression, concentration of hydrogen peroxide and malondialdehyde as well as the total ascorbic acid of the plants were monitored. Moreover, bacterial production of indole-3-acetic acid (IAA) was measured in order to investigate H. paralvei's influence on plant growth. In general, the addition of phenol elevated all biochemical parameters in L. minor except AsA and total soluble proteins. Phenol as well as bacteria influenced the expression of guaiacol peroxidase. Different isoforms were associated with phenol compared to isoforms expressed in phenol-free medium. Considering that duckweeds showed increased antioxidative parameters in the presence of phenol, it can be assumed that the measured parameters might be involved in the plant's defense system. H. paralvei is an IAA producer and its presence in the rhizosphere of duckweeds decreased the oxidative stress of the plants, which can be taken as evidence that this bacterial strain acts protectively on the plants during phenol exposure.",
journal = "Antioxidants",
title = "Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3",
doi = "10.3390/antiox10111719"
Radulović, O., Stanković, S., Stanojević, O., Vujčić, Z., Dojnov, B., Trifunović-Momčilov, M.,& Marković, M.. (2021). Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3. in Antioxidants
MDPI., 10(11), 1719.
https://doi.org/10.3390/antiox10111719
Radulović O, Stanković S, Stanojević O, Vujčić Z, Dojnov B, Trifunović-Momčilov M, Marković M. Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3. in Antioxidants. 2021;10(11):1719.
doi:10.3390/antiox10111719 .
Radulović, Olga, Stanković, Slaviša, Stanojević, Olja, Vujčić, Zoran, Dojnov, Biljana, Trifunović-Momčilov, Milana, Marković, Marija, "Antioxidative Responses of Duckweed (Lemna minor L.) to Phenol and Rhizosphere-Associated Bacterial Strain Hafnia paralvei C32-106/3" in Antioxidants, 10, no. 11 (2021):1719,
https://doi.org/10.3390/antiox10111719 . .
Supplementary data for the article: Margetić, A.; Stojanović, S.; Ristović, M.; Vujčić, Z.; Dojnov, B. Fungal Oxidative and Hydrolyzing Enzymes as Designers in the Biological Production of Dietary Fibers from Triticale. LWT 2021, 145, 111291. https://doi.org/10.1016/j.lwt.2021.111291.
T1  - Supplementary data for the article: Margetić, A.; Stojanović, S.; Ristović, M.; Vujčić, Z.; Dojnov, B. Fungal Oxidative and Hydrolyzing Enzymes as Designers in the Biological Production of Dietary Fibers from Triticale. LWT 2021, 145, 111291. https://doi.org/10.1016/j.lwt.2021.111291.
title = "Supplementary data for the article: Margetić, A.; Stojanović, S.; Ristović, M.; Vujčić, Z.; Dojnov, B. Fungal Oxidative and Hydrolyzing Enzymes as Designers in the Biological Production of Dietary Fibers from Triticale. LWT 2021, 145, 111291. https://doi.org/10.1016/j.lwt.2021.111291.",
Margetić, A., Stojanović, S., Ristović, M., Vujčić, Z.,& Dojnov, B.. (2021). Supplementary data for the article: Margetić, A.; Stojanović, S.; Ristović, M.; Vujčić, Z.; Dojnov, B. Fungal Oxidative and Hydrolyzing Enzymes as Designers in the Biological Production of Dietary Fibers from Triticale. LWT 2021, 145, 111291. https://doi.org/10.1016/j.lwt.2021.111291.. in LWT - Food Science and Technology
Elsevier..
Margetić A, Stojanović S, Ristović M, Vujčić Z, Dojnov B. Supplementary data for the article: Margetić, A.; Stojanović, S.; Ristović, M.; Vujčić, Z.; Dojnov, B. Fungal Oxidative and Hydrolyzing Enzymes as Designers in the Biological Production of Dietary Fibers from Triticale. LWT 2021, 145, 111291. https://doi.org/10.1016/j.lwt.2021.111291.. in LWT - Food Science and Technology. 2021;.
Margetić, Aleksandra, Stojanović, Sanja, Ristović, Marina, Vujčić, Zoran, Dojnov, Biljana, "Supplementary data for the article: Margetić, A.; Stojanović, S.; Ristović, M.; Vujčić, Z.; Dojnov, B. Fungal Oxidative and Hydrolyzing Enzymes as Designers in the Biological Production of Dietary Fibers from Triticale. LWT 2021, 145, 111291. https://doi.org/10.1016/j.lwt.2021.111291." in LWT - Food Science and Technology (2021),
Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines
Simić, Stefan; Jeremić, Sanja; Đokić, Lidija; Božić, Nataša; Vujčić, Zoran; Lončar, Nikola L.; Senthamaraikannan, Ramsankar; Babu, Ramesh P.; Opsenica, Igor; Nikodinović-Runić, Jasmina
AU  - Simić, Stefan
AU  - Jeremić, Sanja
AU  - Đokić, Lidija
AU  - Božić, Nataša
AU  - Lončar, Nikola L.
AU  - Senthamaraikannan, Ramsankar
AU  - Babu, Ramesh P.
AU  - Opsenica, Igor
AU  - Nikodinović-Runić, Jasmina
AB  - Biocatalytic oxidations mediated by laccases are gaining importance due to their versatility and beneficial environmental effects. In this study, the oxidation of 1,4-dihydropyridines has been performed using three different types of bacterial laccase-based catalysts: purified laccase from Bacillus licheniformis ATCC 9945a (BliLacc), Escherichia coli whole cells expressing this laccase, and bacterial nanocellulose (BNC) supported BliLacc catalysts. The catalysts based on bacterial laccase were compared to the commercially available Trametes versicolor laccase (TvLacc). The oxidation product of 2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate was obtained within 7–24 h with good yields (70–99%) with all three biocatalysts. The substrate scope was examined with five additional 1,4-dihydropyridines, one of which was oxidized in high yield. Whole-cell biocatalyst was stable when stored for up to 1-month at 4 °C. In addition, evidence has been provided that multicopper oxidase CueO from the E. coli expression host contributed to the oxidation efficiency of the whole-cell biocatalyst. The immobilized whole-cell biocatalyst showed satisfactory activity and retained 37% of its original activity after three biotransformation cycles.
T2  - Enzyme and Microbial Technology
T1  - Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines
DO  - 10.1016/j.enzmictec.2019.109411
author = "Simić, Stefan and Jeremić, Sanja and Đokić, Lidija and Božić, Nataša and Vujčić, Zoran and Lončar, Nikola L. and Senthamaraikannan, Ramsankar and Babu, Ramesh P. and Opsenica, Igor and Nikodinović-Runić, Jasmina",
abstract = "Biocatalytic oxidations mediated by laccases are gaining importance due to their versatility and beneficial environmental effects. In this study, the oxidation of 1,4-dihydropyridines has been performed using three different types of bacterial laccase-based catalysts: purified laccase from Bacillus licheniformis ATCC 9945a (BliLacc), Escherichia coli whole cells expressing this laccase, and bacterial nanocellulose (BNC) supported BliLacc catalysts. The catalysts based on bacterial laccase were compared to the commercially available Trametes versicolor laccase (TvLacc). The oxidation product of 2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate was obtained within 7–24 h with good yields (70–99%) with all three biocatalysts. The substrate scope was examined with five additional 1,4-dihydropyridines, one of which was oxidized in high yield. Whole-cell biocatalyst was stable when stored for up to 1-month at 4 °C. In addition, evidence has been provided that multicopper oxidase CueO from the E. coli expression host contributed to the oxidation efficiency of the whole-cell biocatalyst. The immobilized whole-cell biocatalyst showed satisfactory activity and retained 37% of its original activity after three biotransformation cycles.",
journal = "Enzyme and Microbial Technology",
title = "Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines",
doi = "10.1016/j.enzmictec.2019.109411"
Simić, S., Jeremić, S., Đokić, L., Božić, N., Vujčić, Z., Lončar, N. L., Senthamaraikannan, R., Babu, R. P., Opsenica, I.,& Nikodinović-Runić, J.. (2020). Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines. in Enzyme and Microbial Technology, 132.
https://doi.org/10.1016/j.enzmictec.2019.109411
Simić S, Jeremić S, Đokić L, Božić N, Vujčić Z, Lončar NL, Senthamaraikannan R, Babu RP, Opsenica I, Nikodinović-Runić J. Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines. in Enzyme and Microbial Technology. 2020;132.
doi:10.1016/j.enzmictec.2019.109411 .
Simić, Stefan, Jeremić, Sanja, Đokić, Lidija, Božić, Nataša, Vujčić, Zoran, Lončar, Nikola L., Senthamaraikannan, Ramsankar, Babu, Ramesh P., Opsenica, Igor, Nikodinović-Runić, Jasmina, "Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines" in Enzyme and Microbial Technology, 132 (2020),
https://doi.org/10.1016/j.enzmictec.2019.109411 . .
Supplementary data for article: Simić, S.; Jeremic, S.; Djokic, L.; Božić, N.; Vujčić, Z.; Lončar, N.; Senthamaraikannan, R.; Babu, R.; Opsenica, I. M.; Nikodinovic-Runic, J. Development of an Efficient Biocatalytic System Based on Bacterial Laccase for the Oxidation of Selected 1,4-Dihydropyridines. Enzyme and Microbial Technology 2020, 132. https://doi.org/10.1016/j.enzmictec.2019.109411
T1  - Supplementary data for article: Simić, S.; Jeremic, S.; Djokic, L.; Božić, N.; Vujčić, Z.; Lončar, N.; Senthamaraikannan, R.; Babu, R.; Opsenica, I. M.; Nikodinovic-Runic, J. Development of an Efficient Biocatalytic System Based on Bacterial Laccase for the Oxidation of Selected 1,4-Dihydropyridines. Enzyme and Microbial Technology 2020, 132. https://doi.org/10.1016/j.enzmictec.2019.109411
title = "Supplementary data for article: Simić, S.; Jeremic, S.; Djokic, L.; Božić, N.; Vujčić, Z.; Lončar, N.; Senthamaraikannan, R.; Babu, R.; Opsenica, I. M.; Nikodinovic-Runic, J. Development of an Efficient Biocatalytic System Based on Bacterial Laccase for the Oxidation of Selected 1,4-Dihydropyridines. Enzyme and Microbial Technology 2020, 132. https://doi.org/10.1016/j.enzmictec.2019.109411",
Simić, S., Jeremić, S., Đokić, L., Božić, N., Vujčić, Z., Lončar, N. L., Senthamaraikannan, R., Babu, R. P., Opsenica, I.,& Nikodinović-Runić, J.. (2020). Supplementary data for article: Simić, S.; Jeremic, S.; Djokic, L.; Božić, N.; Vujčić, Z.; Lončar, N.; Senthamaraikannan, R.; Babu, R.; Opsenica, I. M.; Nikodinovic-Runic, J. Development of an Efficient Biocatalytic System Based on Bacterial Laccase for the Oxidation of Selected 1,4-Dihydropyridines. Enzyme and Microbial Technology 2020, 132. https://doi.org/10.1016/j.enzmictec.2019.109411. in Enzyme and Microbial Technology.
Simić S, Jeremić S, Đokić L, Božić N, Vujčić Z, Lončar NL, Senthamaraikannan R, Babu RP, Opsenica I, Nikodinović-Runić J. Supplementary data for article: Simić, S.; Jeremic, S.; Djokic, L.; Božić, N.; Vujčić, Z.; Lončar, N.; Senthamaraikannan, R.; Babu, R.; Opsenica, I. M.; Nikodinovic-Runic, J. Development of an Efficient Biocatalytic System Based on Bacterial Laccase for the Oxidation of Selected 1,4-Dihydropyridines. Enzyme and Microbial Technology 2020, 132. https://doi.org/10.1016/j.enzmictec.2019.109411. in Enzyme and Microbial Technology. 2020;.
Simić, Stefan, Jeremić, Sanja, Đokić, Lidija, Božić, Nataša, Vujčić, Zoran, Lončar, Nikola L., Senthamaraikannan, Ramsankar, Babu, Ramesh P., Opsenica, Igor, Nikodinović-Runić, Jasmina, "Supplementary data for article: Simić, S.; Jeremic, S.; Djokic, L.; Božić, N.; Vujčić, Z.; Lončar, N.; Senthamaraikannan, R.; Babu, R.; Opsenica, I. M.; Nikodinovic-Runic, J. Development of an Efficient Biocatalytic System Based on Bacterial Laccase for the Oxidation of Selected 1,4-Dihydropyridines. Enzyme and Microbial Technology 2020, 132. https://doi.org/10.1016/j.enzmictec.2019.109411" in Enzyme and Microbial Technology (2020),
Characterization of the starch surface binding site on Bacillus paralicheniformis α-amylase
Božić, Nataša; Rozeboom, Henriëtte J.; Lončar, Nikola; Šokarda Slavić, Marinela; Janssen, Dick B.; Vujčić, Zoran
AU  - Rozeboom, Henriëtte J.
AU  - Lončar, Nikola
AU  - Šokarda Slavić, Marinela
AU  - Janssen, Dick B.
AB  - α-Amylase from Bacillus paralicheniformis (BliAmy), belonging to GH13_5 subfamily of glycoside hydrolases, was proven to be a highly efficient raw starch digesting enzyme. The ability of some α-amylases to hydrolyze raw starch is related to the existence of surface binding sites (SBSs) for polysaccharides that can be distant from the active site. Crystallographic studies performed on BliAmy in the apo form and of enzyme bound with different oligosaccharides and oligosaccharide precursors revealed binding of these ligands to one SBS with two amino acids F257 and Y358 mainly involved in complex formation. The role of this SBS in starch binding and degradation was probed by designing enzyme variants mutated in this region (F257A and Y358A). Kinetic studies with different substrates show that starch binding through the SBS is disrupted in the mutants and that F257 and Y358 contributed cumulatively to binding and hydrolysis. Mutation of both sites (F257A/Y358A) resulted in a 5-fold lower efficacy with raw starch as substrate and at least 5.5-fold weaker binding compared to the wild type BliAmy, suggesting that the ability of BliAmy to hydrolyze raw starch with high efficiency is related to the level of its adsorption onto starch granules.
T1  - Characterization of the starch surface binding site on Bacillus paralicheniformis α-amylase
IS  - A
author = "Božić, Nataša and Rozeboom, Henriëtte J. and Lončar, Nikola and Šokarda Slavić, Marinela and Janssen, Dick B. and Vujčić, Zoran",
abstract = "α-Amylase from Bacillus paralicheniformis (BliAmy), belonging to GH13_5 subfamily of glycoside hydrolases, was proven to be a highly efficient raw starch digesting enzyme. The ability of some α-amylases to hydrolyze raw starch is related to the existence of surface binding sites (SBSs) for polysaccharides that can be distant from the active site. Crystallographic studies performed on BliAmy in the apo form and of enzyme bound with different oligosaccharides and oligosaccharide precursors revealed binding of these ligands to one SBS with two amino acids F257 and Y358 mainly involved in complex formation. The role of this SBS in starch binding and degradation was probed by designing enzyme variants mutated in this region (F257A and Y358A). Kinetic studies with different substrates show that starch binding through the SBS is disrupted in the mutants and that F257 and Y358 contributed cumulatively to binding and hydrolysis. Mutation of both sites (F257A/Y358A) resulted in a 5-fold lower efficacy with raw starch as substrate and at least 5.5-fold weaker binding compared to the wild type BliAmy, suggesting that the ability of BliAmy to hydrolyze raw starch with high efficiency is related to the level of its adsorption onto starch granules.",
title = "Characterization of the starch surface binding site on Bacillus paralicheniformis α-amylase",
number = "A",
Božić, N., Rozeboom, H. J., Lončar, N., Šokarda Slavić, M., Janssen, D. B.,& Vujčić, Z.. (2020). Characterization of the starch surface binding site on Bacillus paralicheniformis α-amylase. in International Journal of Biological Macromolecules
Elsevier., 165(A), 1529-1539.
Božić N, Rozeboom HJ, Lončar N, Šokarda Slavić M, Janssen DB, Vujčić Z. Characterization of the starch surface binding site on Bacillus paralicheniformis α-amylase. in International Journal of Biological Macromolecules. 2020;165(A):1529-1539.
Božić, Nataša, Rozeboom, Henriëtte J., Lončar, Nikola, Šokarda Slavić, Marinela, Janssen, Dick B., Vujčić, Zoran, "Characterization of the starch surface binding site on Bacillus paralicheniformis α-amylase" in International Journal of Biological Macromolecules, 165, no. A (2020):1529-1539,
Expression and characterization of a dye-decolorizing peroxidase from pseudomonas fluorescens Pf0-1
Lončar, Nikola L.; Drašković, Natalija; Božić, Nataša; Romero, Elvira; Simić, Stefan; Opsenica, Igor; Vujčić, Zoran; Fraaije, Marco W.
AU  - Drašković, Natalija
AU  - Romero, Elvira
AU  - Fraaije, Marco W.
AB  - The consumption of dyes is increasing worldwide in line with the increase of population and demand for clothes and other colored products. However, the efficiency of dyeing processes is still poor and results in large amounts of colored effluents. It is desired to develop a portfolio of enzymes which can be used for the treatment of colored wastewaters. Herein, we used genome sequence information to discover a dye-decolorizing peroxidase (DyP) from Pseudomonas fluorescens Pf-01. Two genes putatively encoding for DyPs were identified in the respective genome and cloned for expression in Escherichia coli, of which one (Pf DyP B2) could be overexpressed as a soluble protein. Pf DyP B2 shows some typical features known for DyPs which includes the ability to convert dyes at the expense of hydrogen peroxide. Interestingly, t-butyl hydroperoxide could be used as an alternative substrate to hydrogen peroxide. Immobilization of Pf DyP B2 in calcium-alginate beads resulted in a significant increase in stability: Pf DyP B2 retains 80% of its initial activity after 2 h incubation at 50◦ C, while the soluble enzyme is inactivated within minutes. Pf DyP B2 was also tested with aniline and ethyl diazoacetate as substrates. Based on GC-MS analyses, 30% conversion of the starting material was achieved after 65 h at 30◦ C. Importantly, this is the first report of a DyP-catalyzed insertion of a carbene into an N-H bond.
T2  - Catalysts
T1  - Expression and characterization of a dye-decolorizing peroxidase from pseudomonas fluorescens Pf0-1
DO  - 10.3390/catal9050463
author = "Lončar, Nikola L. and Drašković, Natalija and Božić, Nataša and Romero, Elvira and Simić, Stefan and Opsenica, Igor and Vujčić, Zoran and Fraaije, Marco W.",
abstract = "The consumption of dyes is increasing worldwide in line with the increase of population and demand for clothes and other colored products. However, the efficiency of dyeing processes is still poor and results in large amounts of colored effluents. It is desired to develop a portfolio of enzymes which can be used for the treatment of colored wastewaters. Herein, we used genome sequence information to discover a dye-decolorizing peroxidase (DyP) from Pseudomonas fluorescens Pf-01. Two genes putatively encoding for DyPs were identified in the respective genome and cloned for expression in Escherichia coli, of which one (Pf DyP B2) could be overexpressed as a soluble protein. Pf DyP B2 shows some typical features known for DyPs which includes the ability to convert dyes at the expense of hydrogen peroxide. Interestingly, t-butyl hydroperoxide could be used as an alternative substrate to hydrogen peroxide. Immobilization of Pf DyP B2 in calcium-alginate beads resulted in a significant increase in stability: Pf DyP B2 retains 80% of its initial activity after 2 h incubation at 50◦ C, while the soluble enzyme is inactivated within minutes. Pf DyP B2 was also tested with aniline and ethyl diazoacetate as substrates. Based on GC-MS analyses, 30% conversion of the starting material was achieved after 65 h at 30◦ C. Importantly, this is the first report of a DyP-catalyzed insertion of a carbene into an N-H bond.",
journal = "Catalysts",
title = "Expression and characterization of a dye-decolorizing peroxidase from pseudomonas fluorescens Pf0-1",
doi = "10.3390/catal9050463"
Lončar, N. L., Drašković, N., Božić, N., Romero, E., Simić, S., Opsenica, I., Vujčić, Z.,& Fraaije, M. W.. (2019). Expression and characterization of a dye-decolorizing peroxidase from pseudomonas fluorescens Pf0-1. in Catalysts
MDPI., 9(5).
https://doi.org/10.3390/catal9050463
Lončar NL, Drašković N, Božić N, Romero E, Simić S, Opsenica I, Vujčić Z, Fraaije MW. Expression and characterization of a dye-decolorizing peroxidase from pseudomonas fluorescens Pf0-1. in Catalysts. 2019;9(5).
doi:10.3390/catal9050463 .
Lončar, Nikola L., Drašković, Natalija, Božić, Nataša, Romero, Elvira, Simić, Stefan, Opsenica, Igor, Vujčić, Zoran, Fraaije, Marco W., "Expression and characterization of a dye-decolorizing peroxidase from pseudomonas fluorescens Pf0-1" in Catalysts, 9, no. 5 (2019),
https://doi.org/10.3390/catal9050463 . .
Supplementary data for the article: Lončar, N.; Drašković, N.; Božić, N.; Romero, E.; Simić, S.; Opsenica, I.; Vujčić, Z.; Fraaije, M. W. Expression and Characterization of a Dye-Decolorizing Peroxidase from Pseudomonas Fluorescens Pf0-1. Catalysts 2019, 9 (5). https://doi.org/10.3390/catal9050463
T1  - Supplementary data for the article: Lončar, N.; Drašković, N.; Božić, N.; Romero, E.; Simić, S.; Opsenica, I.; Vujčić, Z.; Fraaije, M. W. Expression and Characterization of a Dye-Decolorizing Peroxidase from Pseudomonas Fluorescens Pf0-1. Catalysts 2019, 9 (5). https://doi.org/10.3390/catal9050463
title = "Supplementary data for the article: Lončar, N.; Drašković, N.; Božić, N.; Romero, E.; Simić, S.; Opsenica, I.; Vujčić, Z.; Fraaije, M. W. Expression and Characterization of a Dye-Decolorizing Peroxidase from Pseudomonas Fluorescens Pf0-1. Catalysts 2019, 9 (5). https://doi.org/10.3390/catal9050463",
Lončar, N. L., Drašković, N., Božić, N., Romero, E., Simić, S., Opsenica, I., Vujčić, Z.,& Fraaije, M. W.. (2019). Supplementary data for the article: Lončar, N.; Drašković, N.; Božić, N.; Romero, E.; Simić, S.; Opsenica, I.; Vujčić, Z.; Fraaije, M. W. Expression and Characterization of a Dye-Decolorizing Peroxidase from Pseudomonas Fluorescens Pf0-1. Catalysts 2019, 9 (5). https://doi.org/10.3390/catal9050463. in Catalysts
MDPI..
Lončar NL, Drašković N, Božić N, Romero E, Simić S, Opsenica I, Vujčić Z, Fraaije MW. Supplementary data for the article: Lončar, N.; Drašković, N.; Božić, N.; Romero, E.; Simić, S.; Opsenica, I.; Vujčić, Z.; Fraaije, M. W. Expression and Characterization of a Dye-Decolorizing Peroxidase from Pseudomonas Fluorescens Pf0-1. Catalysts 2019, 9 (5). https://doi.org/10.3390/catal9050463. in Catalysts. 2019;.
Lončar, Nikola L., Drašković, Natalija, Božić, Nataša, Romero, Elvira, Simić, Stefan, Opsenica, Igor, Vujčić, Zoran, Fraaije, Marco W., "Supplementary data for the article: Lončar, N.; Drašković, N.; Božić, N.; Romero, E.; Simić, S.; Opsenica, I.; Vujčić, Z.; Fraaije, M. W. Expression and Characterization of a Dye-Decolorizing Peroxidase from Pseudomonas Fluorescens Pf0-1. Catalysts 2019, 9 (5). https://doi.org/10.3390/catal9050463" in Catalysts (2019),
Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw
Grujić, Marica; Dojnov, Biljana; Potočnik, Ivana; Atanasova, Lea; Duduk, Bojan; Srebotnik, Ewald; Druzhinina, Irirna S.; Kubicek, Christian P.; Vujčić, Zoran
(Springer Link, 2019)
AU  - Grujić, Marica
AU  - Potočnik, Ivana
AU  - Atanasova, Lea
AU  - Duduk, Bojan
AU  - Srebotnik, Ewald
AU  - Druzhinina, Irirna S.
AU  - Kubicek, Christian P.
AB  - Lignocellulosic plant biomass is the world's most abundant carbon source and has consequently attracted attention as a renewable resource for production of biofuels and commodity chemicals that could replace fossil resources. Due to its recalcitrant nature, it must be pretreated by chemical, physical or biological means prior to hydrolysis, introducing additional costs. In this paper, we tested the hypothesis that fungi which thrive on lignocellulosic material (straw, bark or soil) would be efficient in degrading untreated lignocellulose. Wheat straw was used as a model. We developed a fast and simple screening method for cellulase producers and tested one hundred Trichoderma strains isolated from wheat straw. The most potent strain—UB483FTG2/ TUCIM 4455, was isolated from substrate used for mushroom cultivation and was identified as T. guizhouense. After optimization of growth medium, high cellulase activity was already achieved after 72 h of fermentation on raw wheat straw, while the model cellulase overproducing strain T. reesei QM 9414 took 170 h and reached only 45% of the cellulase activity secreted by T. guizhouense. Maximum production levels were 1.1 U/mL (measured with CMC as cellulase substrate) and 0.7 U/mL (β-glucosidase assay). The T. guizhouense cellulase cocktail hydrolyzed raw wheat straw within 35 h. Our study shows that screening for fungi that successfully compete for special substrates in nature will lead to the isolation of strains with qualitatively and quantitatively superior enzymes needed for their digestion which could be used for industrial purposes.
PB  - Springer Link
T2  - World Journal of Microbiology and Biotechnology
T1  - Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw
DO  - 10.1007/s11274-019-2774-y
author = "Grujić, Marica and Dojnov, Biljana and Potočnik, Ivana and Atanasova, Lea and Duduk, Bojan and Srebotnik, Ewald and Druzhinina, Irirna S. and Kubicek, Christian P. and Vujčić, Zoran",
abstract = "Lignocellulosic plant biomass is the world's most abundant carbon source and has consequently attracted attention as a renewable resource for production of biofuels and commodity chemicals that could replace fossil resources. Due to its recalcitrant nature, it must be pretreated by chemical, physical or biological means prior to hydrolysis, introducing additional costs. In this paper, we tested the hypothesis that fungi which thrive on lignocellulosic material (straw, bark or soil) would be efficient in degrading untreated lignocellulose. Wheat straw was used as a model. We developed a fast and simple screening method for cellulase producers and tested one hundred Trichoderma strains isolated from wheat straw. The most potent strain—UB483FTG2/ TUCIM 4455, was isolated from substrate used for mushroom cultivation and was identified as T. guizhouense. After optimization of growth medium, high cellulase activity was already achieved after 72 h of fermentation on raw wheat straw, while the model cellulase overproducing strain T. reesei QM 9414 took 170 h and reached only 45% of the cellulase activity secreted by T. guizhouense. Maximum production levels were 1.1 U/mL (measured with CMC as cellulase substrate) and 0.7 U/mL (β-glucosidase assay). The T. guizhouense cellulase cocktail hydrolyzed raw wheat straw within 35 h. Our study shows that screening for fungi that successfully compete for special substrates in nature will lead to the isolation of strains with qualitatively and quantitatively superior enzymes needed for their digestion which could be used for industrial purposes.",
publisher = "Springer Link",
journal = "World Journal of Microbiology and Biotechnology",
title = "Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw",
doi = "10.1007/s11274-019-2774-y"
Grujić, M., Dojnov, B., Potočnik, I., Atanasova, L., Duduk, B., Srebotnik, E., Druzhinina, I. S., Kubicek, C. P.,& Vujčić, Z.. (2019). Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw. in World Journal of Microbiology and Biotechnology
Springer Link., 35(12).
Grujić M, Dojnov B, Potočnik I, Atanasova L, Duduk B, Srebotnik E, Druzhinina IS, Kubicek CP, Vujčić Z. Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw. in World Journal of Microbiology and Biotechnology. 2019;35(12).
doi:10.1007/s11274-019-2774-y .
Grujić, Marica, Dojnov, Biljana, Potočnik, Ivana, Atanasova, Lea, Duduk, Bojan, Srebotnik, Ewald, Druzhinina, Irirna S., Kubicek, Christian P., Vujčić, Zoran, "Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw" in World Journal of Microbiology and Biotechnology, 35, no. 12 (2019),
https://doi.org/10.1007/s11274-019-2774-y . .
Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins
Nišavić, Marija; Janjić, Goran V.; Hozić, Amela; Petković, Marijana; Milčić, Miloš K.; Vujčić, Zoran; Cindrić, Mario
AU  - Nišavić, Marija
AU  - Janjić, Goran V.
AU  - Hozić, Amela
AU  - Milčić, Miloš K.
AU  - Cindrić, Mario
AB  - Binding of three ruthenium(ii) compounds of general formula mer-[Ru(L3)(N-N)X][Y] (where L3 = 4-chloro-2,2:6,2-terpyridine (Cl-tpy); N-N = 1,2-diaminoethane (en), 1,2-diaminocyclohexane (dach) or 2,2-bipyridine (bipy); X = Cl; Y = Cl) to human serum albumin (HSA) has been investigated by nano-LC/nano-ESI MS and docking studies. A bottom-up proteomics approach has been applied for the structural characterization of metallated proteins and the data were analyzed in both the positive and negative ion mode. The negative ion mode was achieved after the post-column addition of an isopropanol solution of formaldehyde that enabled sample ionization at micro-flow rates. The negative ion mode MS has been proved to be beneficial for the analysis of binding sites on ruthenated protein in terms of ion charge reduction and consequent simplification of target sequence identification based on isotopic differences between ruthenated and non-ruthenated peptides. Moreover, the negative ion mode ESI MS shows the advantage of singly charged ion formation and, unlike MALDI MS, it does not cause complete ligand fragmentation, merging the benefits of each method into a single experiment. Six target sequences were identified for the binding of en and dach compounds, and four sequences for the binding of bipy. All compounds have been found to bind histidine and one aspartate residue. Docking studies showed that the identified sequences are the constituents of five distinct binding sites for en and dach, or two sites for the bipy complex. The selection of binding sites seems to be dependent on the chelate ligand and the form of the complex prior or after hydrolysis of the leaving chloride ligand.
T2  - Metallomics
T1  - Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins
DO  - 10.1039/c7mt00330g
author = "Nišavić, Marija and Janjić, Goran V. and Hozić, Amela and Petković, Marijana and Milčić, Miloš K. and Vujčić, Zoran and Cindrić, Mario",
abstract = "Binding of three ruthenium(ii) compounds of general formula mer-[Ru(L3)(N-N)X][Y] (where L3 = 4-chloro-2,2:6,2-terpyridine (Cl-tpy); N-N = 1,2-diaminoethane (en), 1,2-diaminocyclohexane (dach) or 2,2-bipyridine (bipy); X = Cl; Y = Cl) to human serum albumin (HSA) has been investigated by nano-LC/nano-ESI MS and docking studies. A bottom-up proteomics approach has been applied for the structural characterization of metallated proteins and the data were analyzed in both the positive and negative ion mode. The negative ion mode was achieved after the post-column addition of an isopropanol solution of formaldehyde that enabled sample ionization at micro-flow rates. The negative ion mode MS has been proved to be beneficial for the analysis of binding sites on ruthenated protein in terms of ion charge reduction and consequent simplification of target sequence identification based on isotopic differences between ruthenated and non-ruthenated peptides. Moreover, the negative ion mode ESI MS shows the advantage of singly charged ion formation and, unlike MALDI MS, it does not cause complete ligand fragmentation, merging the benefits of each method into a single experiment. Six target sequences were identified for the binding of en and dach compounds, and four sequences for the binding of bipy. All compounds have been found to bind histidine and one aspartate residue. Docking studies showed that the identified sequences are the constituents of five distinct binding sites for en and dach, or two sites for the bipy complex. The selection of binding sites seems to be dependent on the chelate ligand and the form of the complex prior or after hydrolysis of the leaving chloride ligand.",
journal = "Metallomics",
title = "Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins",
doi = "10.1039/c7mt00330g",
Nišavić, M., Janjić, G. V., Hozić, A., Petković, M., Milčić, M. K., Vujčić, Z.,& Cindrić, M.. (2018). Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins. in Metallomics
Royal Soc Chemistry, Cambridge., 10(4), 587-594.
https://doi.org/10.1039/c7mt00330g
Nišavić M, Janjić GV, Hozić A, Petković M, Milčić MK, Vujčić Z, Cindrić M. Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins. in Metallomics. 2018;10(4):587-594.
doi:10.1039/c7mt00330g
Nišavić, Marija, Janjić, Goran V., Hozić, Amela, Petković, Marijana, Milčić, Miloš K., Vujčić, Zoran, Cindrić, Mario, "Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins" in Metallomics, 10, no. 4 (2018):587-594,
https://doi.org/10.1039/c7mt00330g .,
Supplementary material for the article: Nišavić, M.; Janjić, G. V.; Hozić, A.; Petković, M.; Milčić, M. K.; Vujčić, Z.; Cindrić, M. Positive and Negative Nano-Electrospray Mass Spectrometry of Ruthenated Serum Albumin Supported by Docking Studies: An Integrated Approach towards Defining Metallodrug Binding Sites on Proteins. Metallomics 2018, 10 (4), 587–594. https://doi.org/10.1039/c7mt00330g
T1  - Supplementary material for the article: Nišavić, M.; Janjić, G. V.; Hozić, A.; Petković, M.; Milčić, M. K.; Vujčić, Z.; Cindrić, M. Positive and Negative Nano-Electrospray Mass Spectrometry of Ruthenated Serum Albumin Supported by Docking Studies: An Integrated Approach towards Defining Metallodrug Binding Sites on Proteins. Metallomics 2018, 10 (4), 587–594. https://doi.org/10.1039/c7mt00330g
title = "Supplementary material for the article: Nišavić, M.; Janjić, G. V.; Hozić, A.; Petković, M.; Milčić, M. K.; Vujčić, Z.; Cindrić, M. Positive and Negative Nano-Electrospray Mass Spectrometry of Ruthenated Serum Albumin Supported by Docking Studies: An Integrated Approach towards Defining Metallodrug Binding Sites on Proteins. Metallomics 2018, 10 (4), 587–594. https://doi.org/10.1039/c7mt00330g",
Nišavić, M., Janjić, G. V., Hozić, A., Petković, M., Milčić, M. K., Vujčić, Z.,& Cindrić, M.. (2018). Supplementary material for the article: Nišavić, M.; Janjić, G. V.; Hozić, A.; Petković, M.; Milčić, M. K.; Vujčić, Z.; Cindrić, M. Positive and Negative Nano-Electrospray Mass Spectrometry of Ruthenated Serum Albumin Supported by Docking Studies: An Integrated Approach towards Defining Metallodrug Binding Sites on Proteins. Metallomics 2018, 10 (4), 587–594. https://doi.org/10.1039/c7mt00330g. in Metallomics
Nišavić M, Janjić GV, Hozić A, Petković M, Milčić MK, Vujčić Z, Cindrić M. Supplementary material for the article: Nišavić, M.; Janjić, G. V.; Hozić, A.; Petković, M.; Milčić, M. K.; Vujčić, Z.; Cindrić, M. Positive and Negative Nano-Electrospray Mass Spectrometry of Ruthenated Serum Albumin Supported by Docking Studies: An Integrated Approach towards Defining Metallodrug Binding Sites on Proteins. Metallomics 2018, 10 (4), 587–594. https://doi.org/10.1039/c7mt00330g. in Metallomics. 2018;.
Nišavić, Marija, Janjić, Goran V., Hozić, Amela, Petković, Marijana, Milčić, Miloš K., Vujčić, Zoran, Cindrić, Mario, "Supplementary material for the article: Nišavić, M.; Janjić, G. V.; Hozić, A.; Petković, M.; Milčić, M. K.; Vujčić, Z.; Cindrić, M. Positive and Negative Nano-Electrospray Mass Spectrometry of Ruthenated Serum Albumin Supported by Docking Studies: An Integrated Approach towards Defining Metallodrug Binding Sites on Proteins. Metallomics 2018, 10 (4), 587–594. https://doi.org/10.1039/c7mt00330g" in Metallomics (2018),
Phenol induced physiological stress in hydroponically grown lettuce (Lactuca sativa L.)- Part 2
Tadić, Vojin; Tadić, Jovan; Milošević, Snežana; Cingel, Aleksandar; Prodanović, Olivera; Ćosić, Tatjana; Vujčić, Zoran
(Elsevier Science Bv, Amsterdam, 2018)
AU  - Tadić, Vojin
AU  - Tadić, Jovan
AU  - Milošević, Snežana
AU  - Cingel, Aleksandar
AU  - Prodanović, Olivera
AU  - Ćosić, Tatjana
AB  - In this study we investigated physiological parameters of stress (enzymatic and non-enzymatic) in lettuce (Lactuca sativa L.) and its hairy roots induced by water solution of phenol. Two varieties of lettuce were examined, Ljubljanska ledenka and Nansen. Plants were grown in water with phenol concentration of 200 mgL(-1), during 10 days. We monitored activity of peroxidases, catalases, polyphenol oxidase and superoxide dismutase, as well as proline and chlorophyll content. We observed a decrease in peroxidases, and increase in activity of catalase, polyphenol oxidase and superoxide dismutase compared to control plants. The concentration of praline was constantly increasing in both lettuce varieties over the course of the experiment. We detected an increase in activity of all monitored enzymes, except polyphenol oxidases, in hairy roots. The hydroponic system provides a useful framework for studying the effect of different harmful substances and its elimination. In such a system, as used in this work for the study of physiological processes in antioxidant protection activated when plant was exposed to phenol, lettuce and its hairy roots can be viewed as tools for water remediation.
PB  - Elsevier Science Bv, Amsterdam
T2  - Scientia Horticulturae
T1  - Phenol induced physiological stress in hydroponically grown lettuce (Lactuca sativa L.)- Part 2
DO  - 10.1016/j.scienta.2017.12.024
author = "Tadić, Vojin and Tadić, Jovan and Milošević, Snežana and Cingel, Aleksandar and Prodanović, Olivera and Ćosić, Tatjana and Vujčić, Zoran",
abstract = "In this study we investigated physiological parameters of stress (enzymatic and non-enzymatic) in lettuce (Lactuca sativa L.) and its hairy roots induced by water solution of phenol. Two varieties of lettuce were examined, Ljubljanska ledenka and Nansen. Plants were grown in water with phenol concentration of 200 mgL(-1), during 10 days. We monitored activity of peroxidases, catalases, polyphenol oxidase and superoxide dismutase, as well as proline and chlorophyll content. We observed a decrease in peroxidases, and increase in activity of catalase, polyphenol oxidase and superoxide dismutase compared to control plants. The concentration of praline was constantly increasing in both lettuce varieties over the course of the experiment. We detected an increase in activity of all monitored enzymes, except polyphenol oxidases, in hairy roots. The hydroponic system provides a useful framework for studying the effect of different harmful substances and its elimination. In such a system, as used in this work for the study of physiological processes in antioxidant protection activated when plant was exposed to phenol, lettuce and its hairy roots can be viewed as tools for water remediation.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Scientia Horticulturae",
title = "Phenol induced physiological stress in hydroponically grown lettuce (Lactuca sativa L.)- Part 2",
doi = "10.1016/j.scienta.2017.12.024",
Tadić, V., Tadić, J., Milošević, S., Cingel, A., Prodanović, O., Ćosić, T.,& Vujčić, Z.. (2018). Phenol induced physiological stress in hydroponically grown lettuce (Lactuca sativa L.)- Part 2. in Scientia Horticulturae
Elsevier Science Bv, Amsterdam., 232, 71-83.
https://doi.org/10.1016/j.scienta.2017.12.024
Tadić V, Tadić J, Milošević S, Cingel A, Prodanović O, Ćosić T, Vujčić Z. Phenol induced physiological stress in hydroponically grown lettuce (Lactuca sativa L.)- Part 2. in Scientia Horticulturae. 2018;232:71-83.
doi:10.1016/j.scienta.2017.12.024
Tadić, Vojin, Tadić, Jovan, Milošević, Snežana, Cingel, Aleksandar, Prodanović, Olivera, Ćosić, Tatjana, Vujčić, Zoran, "Phenol induced physiological stress in hydroponically grown lettuce (Lactuca sativa L.)- Part 2" in Scientia Horticulturae, 232 (2018):71-83,
https://doi.org/10.1016/j.scienta.2017.12.024 .,
Application of new insoluble dietary fibres from triticale as supplement in yoghurt - effects on physico-chemical, rheological and quality properties
Miočinović, Jelena; Tomić, Nikola; Dojnov, Biljana; Tomašević, Igor; Stojanović, Sanja; Đekić, Ilija; Vujčić, Zoran
(Wiley, Hoboken, 2018)
AU  - Miočinović, Jelena
AU  - Tomić, Nikola
AU  - Tomašević, Igor
AU  - Đekić, Ilija
AB  - BACKGROUND: The need to increase the daily intake of dietary fibres opens a new chapter in the research of functional foods enriched with fibres. The potential application of an innovative product - insoluble dietary fibres from triticale in yoghurts - was deployed by characterising their food application and evaluating physico-chemical, rheological and sensory properties and was the aim of this research. RESULTS: Detailed characterisations of these fibres are presented for the first time and showed very good hydration properties, optimal pH ( slightly acidic), optimal chemical composition, high antioxidant capacity which was proven by phenolics contents. Besides, these fibres showed negligible calorific value, with no phytates and high antioxidant capacity, mainly from ferulic acid. Therefore they could be successfully added to yoghurt. Enrichment of yoghurt having different milk fat content (1.5 and 2.8% w/w) with triticale insoluble fibre (1.5% and 3.0% w/w) significantly influenced the syneresis level, its apparent viscosity, yield stress and thixotropic behaviour. The overall sensory quality scores indicated that yoghurt enriched with 1.5% triticale insoluble fibres was recognised as 'excellent' and had enhanced antioxidant activity. CONCLUSIONS: Insoluble triticale fibre could therefore be used as a supplement to produce functional yoghurt. (c) 2017 Society of Chemical Industry
PB  - Wiley, Hoboken
T2  - Journal of the Science of Food and Agriculture
T1  - Application of new insoluble dietary fibres from triticale as supplement in yoghurt - effects on physico-chemical, rheological and quality properties
DO  - 10.1002/jsfa.8592
author = "Miočinović, Jelena and Tomić, Nikola and Dojnov, Biljana and Tomašević, Igor and Stojanović, Sanja and Đekić, Ilija and Vujčić, Zoran",
abstract = "BACKGROUND: The need to increase the daily intake of dietary fibres opens a new chapter in the research of functional foods enriched with fibres. The potential application of an innovative product - insoluble dietary fibres from triticale in yoghurts - was deployed by characterising their food application and evaluating physico-chemical, rheological and sensory properties and was the aim of this research. RESULTS: Detailed characterisations of these fibres are presented for the first time and showed very good hydration properties, optimal pH ( slightly acidic), optimal chemical composition, high antioxidant capacity which was proven by phenolics contents. Besides, these fibres showed negligible calorific value, with no phytates and high antioxidant capacity, mainly from ferulic acid. Therefore they could be successfully added to yoghurt. Enrichment of yoghurt having different milk fat content (1.5 and 2.8% w/w) with triticale insoluble fibre (1.5% and 3.0% w/w) significantly influenced the syneresis level, its apparent viscosity, yield stress and thixotropic behaviour. The overall sensory quality scores indicated that yoghurt enriched with 1.5% triticale insoluble fibres was recognised as 'excellent' and had enhanced antioxidant activity. CONCLUSIONS: Insoluble triticale fibre could therefore be used as a supplement to produce functional yoghurt. (c) 2017 Society of Chemical Industry",
publisher = "Wiley, Hoboken",
journal = "Journal of the Science of Food and Agriculture",
title = "Application of new insoluble dietary fibres from triticale as supplement in yoghurt - effects on physico-chemical, rheological and quality properties",
doi = "10.1002/jsfa.8592",
Miočinović, J., Tomić, N., Dojnov, B., Tomašević, I., Stojanović, S., Đekić, I.,& Vujčić, Z.. (2018). Application of new insoluble dietary fibres from triticale as supplement in yoghurt - effects on physico-chemical, rheological and quality properties. in Journal of the Science of Food and Agriculture
Wiley, Hoboken., 98(4), 1291-1299.
Miočinović J, Tomić N, Dojnov B, Tomašević I, Stojanović S, Đekić I, Vujčić Z. Application of new insoluble dietary fibres from triticale as supplement in yoghurt - effects on physico-chemical, rheological and quality properties. in Journal of the Science of Food and Agriculture. 2018;98(4):1291-1299.
doi:10.1002/jsfa.8592
Miočinović, Jelena, Tomić, Nikola, Dojnov, Biljana, Tomašević, Igor, Stojanović, Sanja, Đekić, Ilija, Vujčić, Zoran, "Application of new insoluble dietary fibres from triticale as supplement in yoghurt - effects on physico-chemical, rheological and quality properties" in Journal of the Science of Food and Agriculture, 98, no. 4 (2018):1291-1299,
https://doi.org/10.1002/jsfa.8592 .,
Phenol removal from solution using different varieties of lettuce (Lactuca sativa L.) - Part 1
Tadić, Vojin; Petric, Marija; Uzelac, Branka; Milošević, Snežana; Vujčić, Zoran; Stevanovic, Jasmina; Tadić, Jovan
AU  - Petric, Marija
AU  - Uzelac, Branka
AU  - Stevanovic, Jasmina
AB  - We investigated the removal of phenol from water solutions (200 mg L-1) using two varieties of lettuce (Lactuca sativa L) and their hairy roots. Experiments were done in a hydroponic system where adult plants were grown in phenol solutions for 10 days. The solution was refreshed every two days in order to maintain the constant concentration of phenol. Hairy roots were also cultivated in a solution containing phenol at concentrations varying from 25 to 125 mg L-1 in order to determine the maximum concentration of phenol that can be removed by hairy roots. Both varieties of lettuce reduced the concentration of phenol below the detection limit after six days at the initial phenol concentration of 200 mg L-1. Transformed roots completely removed phenol at the initial concentrations of 100 mg L-1, but were not able to remove phenol at constant concentration above 25 mg L-1. Lettuce plants and hairy roots are excellent candidates for the process of phenol removal from wastewaters. This plant is good choice for bioremediation of water and represents a potentially efficient and inexpensive system for water purification. The performance of lettuce plants and hairy roots to remove phenol from water solutions under real conditions, depleted nutrients or presence of other compounds should be examined further.
T1  - Phenol removal from solution using different varieties of lettuce (Lactuca sativa L.) - Part 1
author = "Tadić, Vojin and Petric, Marija and Uzelac, Branka and Milošević, Snežana and Vujčić, Zoran and Stevanovic, Jasmina and Tadić, Jovan",
abstract = "We investigated the removal of phenol from water solutions (200 mg L-1) using two varieties of lettuce (Lactuca sativa L) and their hairy roots. Experiments were done in a hydroponic system where adult plants were grown in phenol solutions for 10 days. The solution was refreshed every two days in order to maintain the constant concentration of phenol. Hairy roots were also cultivated in a solution containing phenol at concentrations varying from 25 to 125 mg L-1 in order to determine the maximum concentration of phenol that can be removed by hairy roots. Both varieties of lettuce reduced the concentration of phenol below the detection limit after six days at the initial phenol concentration of 200 mg L-1. Transformed roots completely removed phenol at the initial concentrations of 100 mg L-1, but were not able to remove phenol at constant concentration above 25 mg L-1. Lettuce plants and hairy roots are excellent candidates for the process of phenol removal from wastewaters. This plant is good choice for bioremediation of water and represents a potentially efficient and inexpensive system for water purification. The performance of lettuce plants and hairy roots to remove phenol from water solutions under real conditions, depleted nutrients or presence of other compounds should be examined further.",
title = "Phenol removal from solution using different varieties of lettuce (Lactuca sativa L.) - Part 1",
Tadić, V., Petric, M., Uzelac, B., Milošević, S., Vujčić, Z., Stevanovic, J.,& Tadić, J.. (2018). Phenol removal from solution using different varieties of lettuce (Lactuca sativa L.) - Part 1. in Scientia Horticulturae
Elsevier Science Bv, Amsterdam., 231, 210-218.
Tadić V, Petric M, Uzelac B, Milošević S, Vujčić Z, Stevanovic J, Tadić J. Phenol removal from solution using different varieties of lettuce (Lactuca sativa L.) - Part 1. in Scientia Horticulturae. 2018;231:210-218.
Tadić, Vojin, Petric, Marija, Uzelac, Branka, Milošević, Snežana, Vujčić, Zoran, Stevanovic, Jasmina, Tadić, Jovan, "Phenol removal from solution using different varieties of lettuce (Lactuca sativa L.) - Part 1" in Scientia Horticulturae, 231 (2018):210-218,
Enrichment of yoghurt with insoluble dietary fiber from triticale - A sensory perspective
Tomić, Nikola; Dojnov, Biljana; Miočinović, Jelena; Tomašević, Igor; Smigić, Nada; Đekić, Ilija; Vujčić, Zoran
AU  - Smigić, Nada
AB  - Fortification of fermented dairy products with insoluble dietary fiber is an interesting way to increase consumers' fiber intake. The objective of this study was to evaluate the sensory characteristics and consumer acceptance of low-fat unsweetened yoghurt, fortified at levels of 15 and 30 g/kg, with insoluble triticale, wheat or oat fibers. The addition of insoluble triticale fiber resulted in yellowish-brown color, grainy flavor, and pronounced sandiness/grittiness of the fortified yoghurts. The products were classified into the 'very good' quality category, despite the lower quality scores given to the 30 g/kg fiber fortified yoghurts, caused primarily by a gritty/sandy texture and some bitterness. Three distinct consumer subgroups were revealed by the clustering analysis, one of which showed a preference for the triticale-yoghurts. Insoluble dietary fiber from triticale showed promising potential to be used as a fortifying ingredient in the production of fiber-enriched fermented dairy products such as yoghurt.
T2  - LWT -food Science and Technology ( Lebensmittel - Wissenschaft und Technologie)
T1  - Enrichment of yoghurt with insoluble dietary fiber from triticale - A sensory perspective
DO  - 10.1016/j.lwt.2017.02.008
author = "Tomić, Nikola and Dojnov, Biljana and Miočinović, Jelena and Tomašević, Igor and Smigić, Nada and Đekić, Ilija and Vujčić, Zoran",
abstract = "Fortification of fermented dairy products with insoluble dietary fiber is an interesting way to increase consumers' fiber intake. The objective of this study was to evaluate the sensory characteristics and consumer acceptance of low-fat unsweetened yoghurt, fortified at levels of 15 and 30 g/kg, with insoluble triticale, wheat or oat fibers. The addition of insoluble triticale fiber resulted in yellowish-brown color, grainy flavor, and pronounced sandiness/grittiness of the fortified yoghurts. The products were classified into the 'very good' quality category, despite the lower quality scores given to the 30 g/kg fiber fortified yoghurts, caused primarily by a gritty/sandy texture and some bitterness. Three distinct consumer subgroups were revealed by the clustering analysis, one of which showed a preference for the triticale-yoghurts. Insoluble dietary fiber from triticale showed promising potential to be used as a fortifying ingredient in the production of fiber-enriched fermented dairy products such as yoghurt.",
journal = "LWT -food Science and Technology ( Lebensmittel - Wissenschaft und Technologie)",
title = "Enrichment of yoghurt with insoluble dietary fiber from triticale - A sensory perspective",
doi = "10.1016/j.lwt.2017.02.008",
Tomić, N., Dojnov, B., Miočinović, J., Tomašević, I., Smigić, N., Đekić, I.,& Vujčić, Z.. (2017). Enrichment of yoghurt with insoluble dietary fiber from triticale - A sensory perspective. in LWT -food Science and Technology ( Lebensmittel - Wissenschaft und Technologie)
Elsevier Science Bv, Amsterdam., 80, 59-66.
https://doi.org/10.1016/j.lwt.2017.02.008
Tomić N, Dojnov B, Miočinović J, Tomašević I, Smigić N, Đekić I, Vujčić Z. Enrichment of yoghurt with insoluble dietary fiber from triticale - A sensory perspective. in LWT -food Science and Technology ( Lebensmittel - Wissenschaft und Technologie). 2017;80:59-66.
doi:10.1016/j.lwt.2017.02.008
Tomić, Nikola, Dojnov, Biljana, Miočinović, Jelena, Tomašević, Igor, Smigić, Nada, Đekić, Ilija, Vujčić, Zoran, "Enrichment of yoghurt with insoluble dietary fiber from triticale - A sensory perspective" in LWT -food Science and Technology ( Lebensmittel - Wissenschaft und Technologie), 80 (2017):59-66,
https://doi.org/10.1016/j.lwt.2017.02.008 .,
Enrichment of yoghurt with insoluble dietary fiber from triticale – A sensory perspective
T1  - Enrichment of yoghurt with insoluble dietary fiber from triticale – A sensory perspective
title = "Enrichment of yoghurt with insoluble dietary fiber from triticale – A sensory perspective",
doi = "10.1016/j.lwt.2017.02.008"
Tomić, N., Dojnov, B., Miočinović, J., Tomašević, I., Smigić, N., Đekić, I.,& Vujčić, Z.. (2017). Enrichment of yoghurt with insoluble dietary fiber from triticale – A sensory perspective. in LWT - Food Science and Technology
Tomić N, Dojnov B, Miočinović J, Tomašević I, Smigić N, Đekić I, Vujčić Z. Enrichment of yoghurt with insoluble dietary fiber from triticale – A sensory perspective. in LWT - Food Science and Technology. 2017;80:59-66.
doi:10.1016/j.lwt.2017.02.008 .
Tomić, Nikola, Dojnov, Biljana, Miočinović, Jelena, Tomašević, Igor, Smigić, Nada, Đekić, Ilija, Vujčić, Zoran, "Enrichment of yoghurt with insoluble dietary fiber from triticale – A sensory perspective" in LWT - Food Science and Technology, 80 (2017):59-66,
https://doi.org/10.1016/j.lwt.2017.02.008 . .
Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes
Janović, Barbara; Collins, Andrew R.; Vujčić, Zoran; Vujčić, Miroslava
AU  - Janović, Barbara
AU  - Collins, Andrew R.
AU  - Vujčić, Miroslava
AB  - The aim of this study was to investigate the impact of dyes on DNA before and after enzymatic decolorization by acidic horseradish peroxidase (HRP-A). The comet assay is easy and feasible method widely used to measure DNA damage and repair. The medium-throughput comet assay was employed for assessment of genotoxic effects of 8 dyes in BEAS-2B cells. We have incorporated a digestion with bacterial endonuclease (formamidopyrimidine DNA glycosylase, FPG) to detect oxidized bases in the case of single and double azo dyes, Orange II (OR2) and Amido Black 10B (AB), respectively. This allowed detection 8-oxo7,8-dihydroguanine, one of most abundant oxidized bases in nuclear DNA. In the case of AB there was no indication of DNA damage, either strand brakes or FPG-sensitive sites before and after decolorization. The OR2 induced DNA damage (in terms of percentage of DNA in comet tails). Also, the frequency of FPG-sensitive sites increased with OR2 concentration. After decolorization no DNA damaging effects was seen at all. The interaction studies of OR2 and AB, before and after decolorization, with calf thymus DNA has been investigated by absorption and fluorescence spectroscopy. The results provide support for the idea that in some cases enzymatic decolorization contributes to lower genotoxicity potential. (C) 2016 Elsevier B.V. All rights reserved.
T2  - Journal of Hazardous Materials
T1  - Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes
DO  - 10.1016/j.jhazmat.2016.09.037
author = "Janović, Barbara and Collins, Andrew R. and Vujčić, Zoran and Vujčić, Miroslava",
abstract = "The aim of this study was to investigate the impact of dyes on DNA before and after enzymatic decolorization by acidic horseradish peroxidase (HRP-A). The comet assay is easy and feasible method widely used to measure DNA damage and repair. The medium-throughput comet assay was employed for assessment of genotoxic effects of 8 dyes in BEAS-2B cells. We have incorporated a digestion with bacterial endonuclease (formamidopyrimidine DNA glycosylase, FPG) to detect oxidized bases in the case of single and double azo dyes, Orange II (OR2) and Amido Black 10B (AB), respectively. This allowed detection 8-oxo7,8-dihydroguanine, one of most abundant oxidized bases in nuclear DNA. In the case of AB there was no indication of DNA damage, either strand brakes or FPG-sensitive sites before and after decolorization. The OR2 induced DNA damage (in terms of percentage of DNA in comet tails). Also, the frequency of FPG-sensitive sites increased with OR2 concentration. After decolorization no DNA damaging effects was seen at all. The interaction studies of OR2 and AB, before and after decolorization, with calf thymus DNA has been investigated by absorption and fluorescence spectroscopy. The results provide support for the idea that in some cases enzymatic decolorization contributes to lower genotoxicity potential. (C) 2016 Elsevier B.V. All rights reserved.",
journal = "Journal of Hazardous Materials",
title = "Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes",
doi = "10.1016/j.jhazmat.2016.09.037"
Janović, B., Collins, A. R., Vujčić, Z.,& Vujčić, M.. (2017). Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes. in Journal of Hazardous Materials
https://doi.org/10.1016/j.jhazmat.2016.09.037
Janović B, Collins AR, Vujčić Z, Vujčić M. Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes. in Journal of Hazardous Materials. 2017;321:576-585.
doi:10.1016/j.jhazmat.2016.09.037 .
Janović, Barbara, Collins, Andrew R., Vujčić, Zoran, Vujčić, Miroslava, "Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes" in Journal of Hazardous Materials, 321 (2017):576-585,
https://doi.org/10.1016/j.jhazmat.2016.09.037 . .
Supplementary material for the article: Janović, B. S.; Collins, A. R.; Vujčić, Z. M.; Vujčić, M. T. Acidic Horseradish Peroxidase Activity Abolishes Genotoxicity of Common Dyes. Journal of Hazardous Materials 2017, 321, 576–585. https://doi.org/10.1016/j.jhazmat.2016.09.037
T1  - Supplementary material for the article:           Janović, B. S.; Collins, A. R.; Vujčić, Z. M.; Vujčić, M. T. Acidic Horseradish Peroxidase Activity Abolishes Genotoxicity of Common Dyes. Journal of Hazardous Materials 2017, 321, 576–585. https://doi.org/10.1016/j.jhazmat.2016.09.037
title = "Supplementary material for the article:           Janović, B. S.; Collins, A. R.; Vujčić, Z. M.; Vujčić, M. T. Acidic Horseradish Peroxidase Activity Abolishes Genotoxicity of Common Dyes. Journal of Hazardous Materials 2017, 321, 576–585. https://doi.org/10.1016/j.jhazmat.2016.09.037",
Janović, B., Collins, A. R., Vujčić, Z.,& Vujčić, M.. (2017). Supplementary material for the article:           Janović, B. S.; Collins, A. R.; Vujčić, Z. M.; Vujčić, M. T. Acidic Horseradish Peroxidase Activity Abolishes Genotoxicity of Common Dyes. Journal of Hazardous Materials 2017, 321, 576–585. https://doi.org/10.1016/j.jhazmat.2016.09.037. in Journal of Hazardous Materials
Elsevier Science Bv, Amsterdam..
Janović B, Collins AR, Vujčić Z, Vujčić M. Supplementary material for the article:           Janović, B. S.; Collins, A. R.; Vujčić, Z. M.; Vujčić, M. T. Acidic Horseradish Peroxidase Activity Abolishes Genotoxicity of Common Dyes. Journal of Hazardous Materials 2017, 321, 576–585. https://doi.org/10.1016/j.jhazmat.2016.09.037. in Journal of Hazardous Materials. 2017;.
Janović, Barbara, Collins, Andrew R., Vujčić, Zoran, Vujčić, Miroslava, "Supplementary material for the article:           Janović, B. S.; Collins, A. R.; Vujčić, Z. M.; Vujčić, M. T. Acidic Horseradish Peroxidase Activity Abolishes Genotoxicity of Common Dyes. Journal of Hazardous Materials 2017, 321, 576–585. https://doi.org/10.1016/j.jhazmat.2016.09.037" in Journal of Hazardous Materials (2017),
doi = "10.1016/j.jhazmat.2016.09.037",
doi:10.1016/j.jhazmat.2016.09.037
https://doi.org/10.1016/j.jhazmat.2016.09.037 .,
Tailor-made biocatalysts based on scarcely studied acidic horseradish peroxidase for biodegradation of reactive dyes
Janović, Barbara; Vicovac, Milica Lj. Micic; Vujčić, Zoran; Vujčić, Miroslava
(Springer Heidelberg, Heidelberg, 2017)
AU  - Vicovac, Milica Lj. Micic
AB  - Peroxidases (EC 1.11.1.7) have enormous biotechnological applications. Usage of more abundant, basic isoforms of peroxidases in diagnostic kits and/or in immunochemistry has led to under exploitation and disregard of horseradish peroxidase (HRP) acidic isoforms. Therefore, acidic horseradish peroxidase (HRP-A) isoenzymewas used for the preparation of a biocatalyst with improved ability in dye decolorization. Ten biocatalysts were prepared by covalent binding of enzyme to chitosan and alginate, adsorption followed by cross-linking on inorganic support (aluminum oxide), and encapsulation in spherical calcium alginate beads via polyethylene glycol. Model dyes of 50 to 175 mg l(-1) were removed by the biocatalysts. Among the tested biocatalysts, the three with the highest specific activity and biodegradation rate were further studied (Chitosan-HRP, Al-GelHRP and Al-HRP-Gel). The impact of hydrogen peroxide concentration on dye decolorization was examined on the Chitosan-HRP biocatalyst, since the HRP is susceptible to inhibition/inactivation by high H2O2. On the other hand, H2O2 is needed as a co-substrate for the HRP, and the H2O2/dye ratio can greatly influence decolorization efficiency. Concentrations of H2O2 ranging from 0.22 to 4.4 mM showed no difference in terms of impact on the biocatalyst decolorization efficiency. The high decolorization efficiency of the biocatalysts was validated by the removal of 25 and 100 mg l(-1) anthraquinone (Remazol Brilliant Blue R (RBBR)), triphenylmethane (Coomassie Brilliant Blue CBB)), acridine (Acridine Orange (AO)), and formazan metal complex dye (Reactive Blue 52 (RB52)). After the seven consecutive decolorization cycles, the decolorization was still 53, 78, and 67% of the initial dye for the Al-HRP-Gel, Al-Gel-HRP, and Chitosan-HRP immobilizate, respectively. The results obtained showed potential of otherwise neglected acidic HRP isoforms as a cost-effective biocatalyst with significant potential in wastewater dyestuff treatment.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Tailor-made biocatalysts based on scarcely studied acidic horseradish peroxidase for biodegradation of reactive dyes
author = "Janović, Barbara and Vicovac, Milica Lj. Micic and Vujčić, Zoran and Vujčić, Miroslava",
abstract = "Peroxidases (EC 1.11.1.7) have enormous biotechnological applications. Usage of more abundant, basic isoforms of peroxidases in diagnostic kits and/or in immunochemistry has led to under exploitation and disregard of horseradish peroxidase (HRP) acidic isoforms. Therefore, acidic horseradish peroxidase (HRP-A) isoenzymewas used for the preparation of a biocatalyst with improved ability in dye decolorization. Ten biocatalysts were prepared by covalent binding of enzyme to chitosan and alginate, adsorption followed by cross-linking on inorganic support (aluminum oxide), and encapsulation in spherical calcium alginate beads via polyethylene glycol. Model dyes of 50 to 175 mg l(-1) were removed by the biocatalysts. Among the tested biocatalysts, the three with the highest specific activity and biodegradation rate were further studied (Chitosan-HRP, Al-GelHRP and Al-HRP-Gel). The impact of hydrogen peroxide concentration on dye decolorization was examined on the Chitosan-HRP biocatalyst, since the HRP is susceptible to inhibition/inactivation by high H2O2. On the other hand, H2O2 is needed as a co-substrate for the HRP, and the H2O2/dye ratio can greatly influence decolorization efficiency. Concentrations of H2O2 ranging from 0.22 to 4.4 mM showed no difference in terms of impact on the biocatalyst decolorization efficiency. The high decolorization efficiency of the biocatalysts was validated by the removal of 25 and 100 mg l(-1) anthraquinone (Remazol Brilliant Blue R (RBBR)), triphenylmethane (Coomassie Brilliant Blue CBB)), acridine (Acridine Orange (AO)), and formazan metal complex dye (Reactive Blue 52 (RB52)). After the seven consecutive decolorization cycles, the decolorization was still 53, 78, and 67% of the initial dye for the Al-HRP-Gel, Al-Gel-HRP, and Chitosan-HRP immobilizate, respectively. The results obtained showed potential of otherwise neglected acidic HRP isoforms as a cost-effective biocatalyst with significant potential in wastewater dyestuff treatment.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Tailor-made biocatalysts based on scarcely studied acidic horseradish peroxidase for biodegradation of reactive dyes",
Janović, B., Vicovac, M. Lj. M., Vujčić, Z.,& Vujčić, M.. (2017). Tailor-made biocatalysts based on scarcely studied acidic horseradish peroxidase for biodegradation of reactive dyes. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 24(4), 3923-3933.
Janović B, Vicovac MLM, Vujčić Z, Vujčić M. Tailor-made biocatalysts based on scarcely studied acidic horseradish peroxidase for biodegradation of reactive dyes. in Environmental Science and Pollution Research. 2017;24(4):3923-3933.
doi:10.1007/s11356-016-8100-4
Janović, Barbara, Vicovac, Milica Lj. Micic, Vujčić, Zoran, Vujčić, Miroslava, "Tailor-made biocatalysts based on scarcely studied acidic horseradish peroxidase for biodegradation of reactive dyes" in Environmental Science and Pollution Research, 24, no. 4 (2017):3923-3933,
https://doi.org/10.1007/s11356-016-8100-4 .,
Comparative study of stability of soluble and cell wall invertase from Saccharomyces cerevisiae
Margetić, Aleksandra; Vujčić, Zoran
(Taylor & Francis Inc, Philadelphia, 2017)
AB  - Yeast Saccharomyces cerevisiae is the most significant source of enzyme invertase. It is mainly used in the food industry as a soluble or immobilized enzyme. The greatest amount of invertase is located in the periplasmic space in yeast. In this work, it was isolated into two forms of enzyme from yeast S. cerevisiae cell, soluble and cell wall invertase (CWI). Both forms of enzyme showed same temperature optimum (60 degrees C), similar pH optimum, and kinetic parameters. The significant difference between these biocatalysts was observed in their thermal stability, stability in urea and methanol solution. At 60 degrees C, CWI had 1.7 times longer half-life than soluble enzyme, while at 70 degrees C CWI showed 8.7 times longer half-life than soluble enzyme. After 2-hr of incubation in 8M urea solution, soluble invertase and CWI retained 10 and 60% of its initial activity, respectively. During 22hr of incubation of both enzymes in 30 and 40% methanol, soluble invertase was completely inactivated, while CWI changed its activity within the experimental error. Therefore, soluble invertase and CWI have not shown any substantial difference, but CWI showed better thermal stability and stability in some of the typical protein-denaturing agents.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Preparative Biochemistry and Biotechnology
T1  - Comparative study of stability of soluble and cell wall invertase from Saccharomyces cerevisiae
DO  - 10.1080/10826068.2016.1244683
author = "Margetić, Aleksandra and Vujčić, Zoran",
abstract = "Yeast Saccharomyces cerevisiae is the most significant source of enzyme invertase. It is mainly used in the food industry as a soluble or immobilized enzyme. The greatest amount of invertase is located in the periplasmic space in yeast. In this work, it was isolated into two forms of enzyme from yeast S. cerevisiae cell, soluble and cell wall invertase (CWI). Both forms of enzyme showed same temperature optimum (60 degrees C), similar pH optimum, and kinetic parameters. The significant difference between these biocatalysts was observed in their thermal stability, stability in urea and methanol solution. At 60 degrees C, CWI had 1.7 times longer half-life than soluble enzyme, while at 70 degrees C CWI showed 8.7 times longer half-life than soluble enzyme. After 2-hr of incubation in 8M urea solution, soluble invertase and CWI retained 10 and 60% of its initial activity, respectively. During 22hr of incubation of both enzymes in 30 and 40% methanol, soluble invertase was completely inactivated, while CWI changed its activity within the experimental error. Therefore, soluble invertase and CWI have not shown any substantial difference, but CWI showed better thermal stability and stability in some of the typical protein-denaturing agents.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Preparative Biochemistry and Biotechnology",
title = "Comparative study of stability of soluble and cell wall invertase from Saccharomyces cerevisiae",
Margetić, A.,& Vujčić, Z.. (2017). Comparative study of stability of soluble and cell wall invertase from Saccharomyces cerevisiae. in Preparative Biochemistry and Biotechnology
Taylor & Francis Inc, Philadelphia., 47(3), 305-311.
Margetić A, Vujčić Z. Comparative study of stability of soluble and cell wall invertase from Saccharomyces cerevisiae. in Preparative Biochemistry and Biotechnology. 2017;47(3):305-311.
Margetić, Aleksandra, Vujčić, Zoran, "Comparative study of stability of soluble and cell wall invertase from Saccharomyces cerevisiae" in Preparative Biochemistry and Biotechnology, 47, no. 3 (2017):305-311,
https://doi.org/10.1080/10826068.2016.1244683 .,
Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base
Petric, Marija; Subotic, Angelina; Jevremovic, Sladana; Trifunović-Momcilov, Milana; Tadić, Vojin; Grujić, Marica; Vujčić, Zoran
(Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd, 2017)
AU  - Subotic, Angelina
AU  - Jevremovic, Sladana
AU  - Trifunović-Momcilov, Milana
AB  - The aim of this study was to determine the enzymatic profile of esterases and peroxidases during early stages of somatic embryogenesis of Fritillaria meleagris L. Somatic embryogenesis was induced using the leaf base as explant on a medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D). Zymography showed the presence of different moieties, six isoforms of esterases and peroxidases, during morphogenesis as compared to control explants. One isoform of esterases was detected only during the process of somatic embryogenesis, and one isoform was detected in control explants. Analysis of esterases with 1-naphthyl butyrate proved that esterases, which participate in somatic embryogenesis of F. meleagris, belong to the family of aryl esterases. For the first time it was proved that five isoforms of esterases, which are involved in morphogenesis of F. meleagris, belong to the family of aryl esterases, while two isoforms are carboxyl esterases. One isoform of carboxyl esterases was visible in control explants. This is also the first description of peroxidases during the morphogenetic process, and of the difference between aryl and carboxyl esterases. More isoforms of esterases during morphogenesis as compared to control explants are probably responsible for some early physiological process during somatic embryogenesis of F. meleagris.
PB  - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
T2  - Archives of biological sciences
T1  - Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base
DO  - 10.2298/ABS161130007P
author = "Petric, Marija and Subotic, Angelina and Jevremovic, Sladana and Trifunović-Momcilov, Milana and Tadić, Vojin and Grujić, Marica and Vujčić, Zoran",
abstract = "The aim of this study was to determine the enzymatic profile of esterases and peroxidases during early stages of somatic embryogenesis of Fritillaria meleagris L. Somatic embryogenesis was induced using the leaf base as explant on a medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D). Zymography showed the presence of different moieties, six isoforms of esterases and peroxidases, during morphogenesis as compared to control explants. One isoform of esterases was detected only during the process of somatic embryogenesis, and one isoform was detected in control explants. Analysis of esterases with 1-naphthyl butyrate proved that esterases, which participate in somatic embryogenesis of F. meleagris, belong to the family of aryl esterases. For the first time it was proved that five isoforms of esterases, which are involved in morphogenesis of F. meleagris, belong to the family of aryl esterases, while two isoforms are carboxyl esterases. One isoform of carboxyl esterases was visible in control explants. This is also the first description of peroxidases during the morphogenetic process, and of the difference between aryl and carboxyl esterases. More isoforms of esterases during morphogenesis as compared to control explants are probably responsible for some early physiological process during somatic embryogenesis of F. meleagris.",
publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd",
journal = "Archives of biological sciences",
title = "Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base",
doi = "10.2298/ABS161130007P",
Petric, M., Subotic, A., Jevremovic, S., Trifunović-Momcilov, M., Tadić, V., Grujić, M.,& Vujčić, Z.. (2017). Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base. in Archives of biological sciences
Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 69(4), 619-625.
https://doi.org/10.2298/ABS161130007P
Petric M, Subotic A, Jevremovic S, Trifunović-Momcilov M, Tadić V, Grujić M, Vujčić Z. Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base. in Archives of biological sciences. 2017;69(4):619-625.
doi:10.2298/ABS161130007P
Petric, Marija, Subotic, Angelina, Jevremovic, Sladana, Trifunović-Momcilov, Milana, Tadić, Vojin, Grujić, Marica, Vujčić, Zoran, "Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base" in Archives of biological sciences, 69, no. 4 (2017):619-625,
https://doi.org/10.2298/ABS161130007P .,


text:
Moritz David (geboren am 18. Dezember 1875 in Gimbsheim; gestorben am 16. Januar 1956 in Manchester) war ein deutscher Rabbiner in Bochum.

Leben
David war ein Sohn des Kaufmanns Emil David II. und dessen Ehefrau Amalie (geborene Rüb). Er besuchte zunächst von Ostern 1885 bis Ostern 1894 das Gymnasium in Worms und studierte anschließend von 1894 bis 1896 Theologie am Jüdisch-Theologischen Seminar (Fraenckel'sche Stiftung) in Breslau, wo er unter anderem Vorlesungen bei Markus Brann hörte. Er belegte zugleich ein Studium der semitischen Geschichte, Philologie und Philosophie an der dortigen Universität. Im Herbst 1896 wechselte er an die philosophische Fakultät der Friedrich-Alexander-Universität nach Erlangen, wo er seine Dissertation Das Targum scheni zum Buch Esther ablieferte und am 22. Februar 1897 promoviert wurde.

1901 wurde er als erster Rabbiner der Synagogengemeinde Bochum berufen und blieb dort bis zu seinem freiwilligen Ruhestand im Jahr 1934. 1938 betreute er kurz das vakante Rabbinat in Dortmund, bevor er mit Hilfe von Leo Baeck nach England emigrierte. Er starb am 16. Januar 1956 in Manchester.

1959 wurde durch einige Mitglieder der Synagogengemeinde Bochum eine Gedenktafel für Moritz David auf dem jüdischen Friedhof an der Wasserstraße enthüllt.

Schriften (Auswahl)

Einzelnachweise

Rabbiner (Deutschland)
Emigrant aus dem Deutschen Reich zur Zeit des Nationalsozialismus
Person des Judentums (Bochum)
Person des Judentums (Dortmund)
Deutscher
Geboren 1875
Gestorben 1956
Mann

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The exhibition of masterpieces of Islamic art from the Aga Khan Collection organised by the Aga Khan Trust for Culture which will be presented at the Temporary Exhibition Gallery of the Calouste Gulbenkian Museum, reveals 1000 years of artistic production from the 9th to the 19th century. The pieces are representative of a wide geographical region, ranging from Spain to Indonesia.
Among the works in the exhibition, the visitor will find illuminated manuscripts, metal and glass objects, textiles, jewellery and paintings, many of which have already been shown in Parma, London and Paris.
O great collection, come home to papa and mama, we're waiting for you here in Toronto……….


Official music video for "Easy Mistake" from David Corley's debut album "Available Light". Directed by Juno Award-winning Sean Michael Turrell, featuring David Corley, Sarah McDermott, Kate Fenner and Hugh Christopher Brown.
Live at the Velvet Lounge, Washington, DC. Video by Aileen Nowlan.
Official video for the title track for Toronto singer-songwriter Suzanne Jarvie's debut album, Spiral Road, produced by multi-instrumentalist Hugh Christopher Brown. Video was created by Jason van Bruggen and was filmed in Arizona and New Mexico.
Transistor Heart – from Too Many Sisters – Non Barking Dog – Recorded at Graffiti's in Kensington Market, Toronto, April 2014. Too Many Sisters was a duo with Stephen and vocalist/violinist Carla MacNeil.
Trainwrecks by Chris Brown and Kate Fenner from the album Geronimo.
Video and song by Kate Fenner, Canadian singer/songwriter.


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491st Bombardment Group
Constituted as 491st Bombardment Group (Heavy) on 14 Sep 1943. Activated on 1 Oct 1943. Trained for combat with B-24's. On 1 Jan 1944 the group, less the air echelon, was transferred without personnel and equipment to England, where personnel were assigned later. The air echelon continued to train in the US until it joined the group in England in May 1944. Served in combat with Eighth AF until the end of Apr 1945. Began operations early in Jun 1944 and attacked airfields, bridges, and coastal defenses both preceding and during the invasion of Normandy. Then concentrated its attacks on strategic objectives in Germany, striking communications centers, oil refineries, storage depots, industrial areas, shipyards, and other targets in such places as Berlin, Hamburg, Kassel, Cologne, Gelsenkirchen, Bielefeld, Hannover, and Magdeburg; on one occasion attacked the headquarters of the German General Staff at Zossen, Germany. While on a mission to bomb an oil refinery at Misburg on 26 Nov 1944, the group was attacked by large numbers of enemy fighters; although about one-half of its planes were destroyed, the remainder fought off the interceptors, successfully bombed the target, and won for the group a DUC. Although engaged primarily in strategic bombardment, the group also supported ground forces at St Lo in Jul 1944; assaulted V-weapon sites and communications lines in France during the summer of 1944; dropped supplies to paratroops on 18 Sep 1944 during the airborne attack in Holland; bombed German supply lines and fortifications during the Battle of the Bulge, Dec 1944-Jan 1945; supported Allied forces in the airborne drop across the Rhine in Mar 1945; and interdicted enemy communications during the Allied drive across Germany in Apr 1945. Returned to the US in Jul. Inactivated on 8 Sep 1945.
Squadrons. 852d: 1943-1945. 853d: 1943-1945. 854th: 1943-1945. 855th: 1943-1945.
Stations. Davis-Monthan Field, Ariz, 1 Oct 1943; El Paso, Tex, 11 Nov 1943; England, 1 Jan 1944; North Pickenham, England, Feb 1944; Metfield, England, Mar 1944; North Pickenham, England, 15 Aug 1944-Jun 1945; McChord Field, Wash, 17 Jul-8 Sep 1945.
Commanders. Col Dwight O Monteith, 10 Oct 1943; Maj Jack G Merrell, 20 Dec 1943; Col Wilson H Banks, 5 Jan 1944; Maj Alex E Burleigh, 19 Jan 1944; Lt Col Jack G Merrell, 29 Jan 1944; Lt Col Carl T Goldenburg, 12 Feb 1944; Col F H Miller, 26 Jun 1944; Col Allen W Reed, c. 20 Oct 1944-1945.
Campaigns. Air Offensive, Europe; Normandy; Northern France; Rhineland; Ardennes-Alsace; Central Europe.
Decorations. Distinguished Unit Citation: Misburg, Germany, 26 Nov 1944.
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LGBTQ health disparity study in B.C. leads to surprise finding
Suicidal thoughts and attempts go down by half for straight males in schools with GSAs.
Oct. 12, 2017 12:30 p.m.
A leading researcher of vulnerable and marginalized adolescents including LGBTQ students said more inclusive school climates — including Gay-Straight Alliances (GSAs) — reduced suicides and suicide attempts by 50 per cent for straight males in B.C. last year.
Dr. Elizabeth M Saewyc called it a "surprise find."
"Suicidal thoughts and attempts actually go down by half [for straight males] in schools with GSAs and inclusive LGBTQ policies across the province," Dr. Saewyc said.
She was initially researching population-focused health disparities for LGBTQ people. In trying to understand the disparities, she recognized that there were much higher rates of bullying, discrimination and stigma reported among LGBTQ people. In fact, the rate of discrimination experienced by students who identify as LGBTQ is three times higher than reported by heterosexual youth.
READ MORE: John White: Bullying leaves deep scars behind
On top of suicides and suicide attempts, myriad other health conditions were notably higher for LGBTQ youth. The reduction of suicide numbers for straight males might actually be tied to inclusive actions that improve conditions and reduce the same statistic for LGBTQ students, Dr. Saewyc surmised.
"A more supportive and inclusive climate creates an overall calm, lessening stress for straight people as well as those who identify within the LGBTQ community," Dr. Saewyc said.
"Homophobic bullying, by boys especially, is a way of policing 'how to be a guy.' They use it to rag on boys who don't fit the classic macho stereotype," Dr. Saewyc said. "What we think might be happening is, when you've got a climate that supports and allows a diverse way of being — that it's OK to be gay and bi and the different ways you can manifest being gendered — it takes pressure off of straight boys, too."
Dr. Saewyc said straight males can approach masculinity without having their credentials as a straight male questioned.
"It creates more breathing room for everyone to be more comfortable with who they are," Dr. Saewyc said.
"Being called gay — or if people think you're gay, even if you're straight — has an effect on mental health," Dr. Saewyc said.
It's the "minority stress" that goes with being a part of a marginalized group.
"We've learned that some of our society gives tacit permission for some kinds of bullying or harassment. People are figuring out gender identity and fitting in… everyone wants to be unique and fit in at the same time. It can be very challenging to not be a part of the group," Dr. Saewyc said.
"People who don't meet stereotypical norms end up being the target for a lot of the bullying and harassment as an 'out' group. Picking on people is a way for some to feel like they are a part of the 'in' group."
When adults in the community don't confront the harassment and don't model supportive and inclusive behaviour, children feel like they have tacit approval. In places where adults are speaking out against LGBTQ people, children feel like they have permission to do the same.
Further, coming out is not an option for some children because their family would not support that admission, Dr. Saewyc said. Those children face the dual dilemma of bullying and the inability to open up to their parents for support.
GSA groups work to change the climate in schools. Dr. Saewyc said when schools have GSAs in place for at least a couple of years, it changes the level of reported discrimination and brings lower levels of the health concerns that are normally the key outcomes of bullying.
"Having the GSAs run by student groups, straight students can step up and be a part of advocating for inclusion and respect," Dr. Saewyc said.
"It's also important to recognize and be an important bystander. If you see someone being teased or threatened because of their gender orientation, step up. For adults, we have to speak out against stigma and discrimination when we see it."
Cyberbullying is another, more recent manifestation for LGBTQ youth.
It is theoretically less harmful because there is no physical abuse, but continuous access through texting and social media means there is nowhere to hide.
"You can't escape it easily. Bullies feel anonymous, and they can immobilize quick a mob of trolls to jump on a particular circumstance," Dr. Saewyc said.
On the other hand, young people who might not get support where they live can find supportive friends and others online.
"Those groups can encourage them or celebrate them for who they really are so they feel safer about coming out, where they might not have that in their local community," Dr. Saewyc said.
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Canadian doctor warns parents to lock up medication so teens don't steal it
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We care about our team members.
Our company began in 1932 as a small scoop shop in Cleveland's MidTown neighborhood.
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At Pierre's Ice Cream Company, culinary craftsmanship meets food science and innovation. We make award-winning classics and original creations from only the finest ingredients. However, producing hundreds of our own treats and private label products is only part of our story.
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Minimum of one (1) year warehouse management experience.
Valid driver's license, CDL Class A or B useful.
Familiarity with automated warehouse management systems and/or Voxware a plus.
If you do not see an available position, please feel free to fill out our online application, which will be kept on file for one year. We will contact you should your qualifications match an opening that becomes available.