text:
Involvement of PhoP-PhoS Homologs in Enterococcus faecalis Virulence
Fang Teng, Ling Wang, Kavindra V. Singh, Barbara E. Murray, George M. Weinstock
Fang Teng
Department of Microbiology and Molecular GeneticsCenter for the Study of Emerging and Reemerging Pathogens, University of Texas Houston Medical School, Houston, Texas 77030
Ling Wang
Department of Microbiology and Molecular Genetics
Kavindra V. Singh
Center for the Study of Emerging and Reemerging Pathogens, University of Texas Houston Medical School, Houston, Texas 77030Division of Infectious Disease, Department of Medicine
Barbara E. Murray
Department of Microbiology and Molecular GeneticsCenter for the Study of Emerging and Reemerging Pathogens, University of Texas Houston Medical School, Houston, Texas 77030Division of Infectious Disease, Department of Medicine
George M. Weinstock
For correspondence: gwstock@bcm.tmc.edu
DOI: 10.1128/IAI.70.4.1991-1996.2002
Eleven PhoP-PhoS homolog pairs were identified by searching the Enterococcus faecalis V583 genome sequence database at The Institute for Genomic Research with the Bacillus subtilis PhoP-PhoS sequences. Each pair appears to be a potential two-component system composed of a response regulator and a sensor kinase. Seven of the homologs were disrupted in E. faecalis strain OG1RF. TX10293, a mutant disrupted in one of these genes (etaR, the first gene of the gene pair designated etaRS), showed delayed killing and a higher 50% lethal dose in a mouse peritonitis model. The predicted EtaR protein sequence showed greatest similarity to LisR of Listeria monocytogenes (77%) and CsrR of Streptococcus pyogenes (70%); EtaS is 53% similar to LisK and 54% similar to CsrS. When grown in vitro, the TX10293 mutant was more sensitive to low pH (pH 3.4) and more resistant to high temperature (55°C) than wild-type OG1RF. In conclusion, many potential two-component systems are identified for E. faecalis, one of which, EtaRS, was shown to be involved in stress response and virulence.
Enterococci are normal flora of the human intestine, found in the feces of most healthy adults. These organisms are also one of the leading causes of nosocomial infections, producing various diseases including bacteremia, urinary tract infections, endocarditis, intra-abdominal and pelvic infections, neonatal infections, and central nervous system infections, among others (15). Seventy to ninety percent of clinical enterococcal isolates are Enterococcus faecalis, with Enterococcus faecium playing an increasingly important role in the past decade.
Bacteria often use two-component systems to control expression of virulence factors in response to environmental changes throughout the various stages of infection. These two-component systems are generally composed of a sensor kinase that recognizes one or more specific environmental signals and a response regulator protein that mediates cell response by regulating expression of specific operons or modulating protein functions (9). The genes encoding the sensor and regulator are often cotranscribed as a single transcript (9). An example is the CsrRS (also called CovRS) system of Streptococcus pyogenes. The CsrRS system is a two-component system that was first identified as a negative regulator of hyaluronic acid capsule synthesis (8, 12). In an attempt to identify potential two-component systems in S. pyogenes, PhoP-PhoS, a global regulatory system in Bacillus subtilis, was used as query, and CsrRS was identified as a PhoPS homolog (6) that negatively regulates the transcription of several virulence factors, including ska (encoding streptokinase), sagA (encoding streptolysin S), speMF (encoding mitogenic factor), and hasA, the first gene in the operon for capsule synthesis (6). The phosphorylated CsrR was then found to be able to bind to the promoters of ska, sagA, speMF, and hasA with different affinities, consistent with its function as a response regulator and its differential regulation of these virulence genes (14). Two-component systems in enterococci have not been well studied, except for the VanRS and VanRBSB systems, which regulate resistance of enterococci to vancomycin (1).
To identify potential two-component regulatory gene pairs, the E. faecalis V583 genome sequence at The Institute for Genomic Research (TIGR) was searched using the sequences of B. subtilis PhoP-PhoS (10). Here we report that disruption of one set of phoPS homologs, etaRS (enterococcal two-component system a), affects the virulence of E. faecalis in a mouse peritonitis model. In vitro, the disruption mutant also demonstrated a rate of survival at high temperature (55°C) and low pH (3.4) that was different from that of wild-type OG1RF.
Bacterial strains and media.The wild-type E. faecalis strain used in this study, OG1RF, has been described previously (16). Escherichia coli was grown in Luria-Bertani broth or agar with appropriate antibiotics at 37°C. Brain heart infusion (BHI) medium (Difco Laboratories, Detroit, Mich.) was used for growth of E. faecalis. SR medium was used for electroporation of E. faecalis. The concentration of kanamycin used for selection was 25 μg/ml for E. coli and 2,000 μg/ml for E. faecalis.
DNA manipulations.DNA amplification (PCR), cloning, and Southern blotting were performed using standard methods (19) unless otherwise described. Transformation of E. coli and electroporation of E. faecalis were as described previously (2, 13).
Construction and analysis of disruption mutants.An internal fragment of the first gene of each of the potential two-component gene pairs was amplified from OG1RF genomic DNA (oligonucleotides used are listed in Table 1) and cloned into the TOPO TA cloning vector (Invitrogen, Carlsbad, Calif.). The fragments were then released from the vector by EcoRI and cloned into pTEX4577 (17), which is a derivative of pBluescript KS(−) and contains a kanamycin resistance gene of gram-positive origin. The resulting constructs were electroporated into E. faecalis OG1RF followed by selection on SR agar plates with 2,000 μg of kanamycin per ml. The correct insertions were confirmed by PCR and Southern blot analysis. Growth curves of OG1RF and mutants and stability of single-crossover disruptions in mutants were determined as described previously (22).
The PhoP-PhoS homologs identified in E. faecalis V583 and outcome of disruption in E. faecalis OG1RF
Mouse peritonitis model.The mouse peritonitis model and the 50% lethal dose (LD50) were described previously (18, 20, 22) and applied in this study with minor modifications: 25% sterile rat fecal extract was used. Determination of Kaplan-Meier survival curves and log rank analysis were performed using StatView (Abacus Concepts, Inc., Berkeley, Calif.).
Acid and heat sensitivity assay.Overnight cultures were inoculated into 10 ml of fresh BHI, and log phase cells (35 Klett units; Klett-Summerson photoelectric colorimeter, Klett MFG Co., Inc., New York, N.Y.) were collected, washed, and resuspended in 1 ml of 10 mM MgSO4, which was kept at 4°C for up to 5 days. On the day of experiment, these cells (in 10 mM MgSO4) were diluted 1:50 into BHI and incubated at 37°C. When log phase was reached (35 Klett units), cells were diluted 1:50 into BHI that had been adjusted to pH 3.4 with 3 M lactic acid and then incubated at 37°C for 40 min (for acid sensitivity assay). For heat sensitivity assay, cells were diluted 1:50 into BHI and incubated at 55°C for 40 min. The starting numbers of cells added to the tubes prepared for acid or heat sensitivity assays were determined by plating serial dilutions on BHI agar plates and incubating overnight. After 40 min, the number of survivors was determined as described above. The percentage of cells surviving was calculated by comparing the starting and the 40-min cell counts. The experiments were performed twice, duplicates were used in each experiment, and the significance of the difference between wild-type OG1RF and the mutant was determined by a t test (for two-group comparisons).
Ethanol and high-salt sensitivity assay.Overnight cultures of OG1RF, TX10293, and TX37200 were diluted 1:50 into BHI with 5% ethanol or 6.5% NaCl and incubated at 37°C. Klett units were measured every 2 h up to 8 h and then at 24 h.
Identification of PhoP-PhoS homologs in E. faecalis.The E. faecalis V583 genome sequence (obtained from TIGR) was searched for potential two-component regulatory gene pairs using the B. subtilis PhoP and PhoS polypeptide sequences to perform BLAST searches. Eleven gene pairs, to which we gave the numerical designations listed in Table 1, were identified. Each gene pair is composed of a gene encoding a potential response regulator and a gene encoding a potential sensor kinase. Sequence analysis also suggested that each gene pair forms a potential operon, although the gene order varies (Table 1). One of the gene pairs encodes the known VanRBSB system which is involved in regulation of VanB-type vancomycin resistance gene expression in E. faecalis (5) and was not studied further.
Inactivation of PhoP-PhoS homologs in E. faecalis OG1RF.V583 has multiple resistances to antibiotic markers of commonly used vectors; thus, OG1RF, a strain commonly used in the laboratory for genetic manipulations, was used to construct disruption mutants. Based on the sequences identified in V583, oligonucleotides (listed in Table 1) were designed to amplify from OG1RF the internal part of the first (upstream) gene of each of the 10 gene pairs studied. We could not amplify the sequence at locus 10285 from either OG1RF or V583, so this gene pair was not studied further. For the sequence at locus 10288, we did not get a PCR product from OG1RF genomic DNA with the designed primers, while amplification from V583 genomic DNA was successful under the same conditions, suggesting that the gene may not be present in OG1RF or may have a different sequence in OG1RF. The PCR products for the other eight genes were obtained and used to make disruption mutants in OG1RF. We isolated seven mutants: TX10276, TX10275, TX10292, TX10293, TX10303, TX37200, and TX10298. Disruption mutation at locus 10299 was not obtained, suggesting the possibility that this gene pair may be essential for bacterial growth.
Growth rates and stability of the mutants.The growth rates of OG1RF and the seven mutants in BHI broth at 37°C were examined. The results showed that all the mutants had similar doubling times in log phase and similar densities at stationary phase. After 24 h, the cell counts of these cultures were comparable for OG1RF and the seven mutants. The colony counts on BHI and BHI-kanamycin were comparable for all the mutants, suggesting that the disruption mutations were stable under this growth condition.
Examination of the two-component system mutants in a mouse peritonitis model.To test if disruption of these potential two-component systems had an effect on the virulence of OG1RF, the seven mutants were examined in a mouse peritonitis model, along with wild-type OG1RF. In the mouse peritonitis model, initial experiments showed that two mutants, TX10293 and TX37200, had higher survival rates than wild-type OG1RF when the number of cells inoculated was the same or slightly greater than that for the wild type (P = 0.009 for TX10293 versus OG1RF and P = 0.022 for TX37200 versus OG1RF; the inocula were 5.0 × 107, 5.0 × 107, and 8.0 × 107 CFU for OG1RF, TX10293, and TX37200) (Fig. 1A). The two sets of genes disrupted in TX10293 and TX37200, respectively, were named etaRS and etbRS (enterococcal two-component systems a and b). The other mutants did not show a significant difference in lethality from that of OG1RF (data not shown). To confirm these results, the mutants TX10293 and TX37200 were further examined in a larger-scale experiment. A group of 12 mice was used for each of the three inocula (based on the optical density at 600 nm and confirmed by cell counts). The etaR mutant, TX10293, again showed a higher percentage of surviving mice and delayed killing compared with that of OG1RF, while the etbS mutant, TX37200, showed a similar trend, but the difference between TX37200 and the wild type, OG1RF, was not significant (P = 0.0013 for TX10293 versus OG1RF, and P = 0.092 for TX37200 versus OG1RF; the inocula were 2.7 × 107, 3.0 × 107, and 3.2 × 107 CFU for OG1RF, TX10293, and TX37200) (Fig. 1B). The LD50s for OG1RF, TX10293, and TX37200 were 1.6 × 107, 7.3 × 107, and 3.9 × 107, respectively. The results suggest that the two-component system EtaRS contributes to virulence of E. faecalis in the mouse peritonitis model. Because of the less consistent results for the etbS mutant, the role of the EtbRS system in E. faecalis virulence is not clear.
Percentages of surviving mice injected with OG1RF, TX10293, or TX37200 over time. (A) The inoculum size and the number of mice used for each strain were as follows: OG1RF, 5.0 × 107 CFU (six mice); TX10293, 5.0 × 107 CFU (six mice); and TX37200, 8.0 × 107 CFU (six mice). (B) The inoculum size and the number of mice used for each strain were as follows: OG1RF, 2.7 × 107 (12 mice); TX10293, 3.0 × 107 CFU (12 mice); TX37200, 3.2 × 107 CFU (12 mice). The LD50 values were calculated based on this experiment (see the text).
The numbers of colonies recovered from the spleens of dead mice receiving the mutants were comparable on BHI agar and on BHI-kanamycin agar plates, indicating that the disruptions were at least relatively stable in vivo. It was observed that TX10293, the etaR mutant, was mucoid on the primary isolation plates when recovered from the spleens, suggesting that the EtaRS system may negatively regulate capsule synthesis of E. faecalis in vivo. The recovered strains were frozen immediately. However, when restreaked, the etaR mutant was not mucoid, suggesting that in vivo conditions were also important for this phenotype or the mucoidy was disadvantageous in vitro.
Sequence analysis of etaRS genes and their encoded proteins.Sequence analysis of the 10293 locus showed that the sensor kinase homolog (etaS) follows the response regulator homolog (etaR), and there are 11 bp between the two open reading frames and no second promoter; thus, they are likely to be cotranscribed. BLAST searches were performed using predicted EtaRS polypeptide sequences, and two regulatory systems, LisRK of Listeria monocytogenes (3) and CsrRS of S. pyogenes (6, 8, 12), showed the highest sequence similarity of all hits to the EtaRS system (Fig. 2). The response regulator LisR showed 68% identity and 77% similarity to EtaR, while CsrR showed 60% identity and 70% similarity to EtaR. The sensor kinase LisK showed 37% identity and 53% similarity to EtaS, while CsrS showed 33% identity and 54% similarity to EtaS. Upstream of etaR, an open reading frame was identified with 76% protein sequence identity and 88% similarity to the 6-phosphogluconate dehydrogenase gene of Lactococcus lactis (SwissPROT accession no. P96789). This open reading frame is transcribed in the same orientation as etaR. Interestingly, upstream of lisR there is also an open reading frame encoding a 6-phosphogluconate homolog, suggesting that lisRK and etaRS have similar gene organizations at their loci.
(A) Alignment of EtaR, LisR, and CsrR polypeptide sequences. (B) Alignment of EtaS, LisK, and CsrS polypeptide sequences. The Pileup program in the GCG software package (Genetics Computer Group, Madison, Wis.) was used to make the alignment, and the GeneDoc software was used for editing and shading (the bottom line shows the consensus sequence).
Survival of mutants TX10293 and TX37200 in low pH and high temperature.To study the environmental cues that the EtaRS and EtbRS might respond to, different conditions were used in vitro to examine the phenotypes of TX10293 and TX37200. No significant difference was detected for growth of OG1RF, TX10293, and TX37200 in BHI with 5% ethanol or 6.5% NaCl (data not shown). When exposed to a low pH (pH 3.4), TX37200 showed a survival rate similar to that of wild-type OG1RF, and the survival rate of TX10293 was approximately 100-fold lower than that of wild-type OG1RF (P = 0.034) (Fig. 3A). When exposed to high temperature (55°C), TX10293 showed about 10-fold greater survival than wild-type OG1RF (P = 0.006), and TX37200 had two- to threefold less survival than OG1RF (P = 0.01) (Fig. 3B). The experiments were performed twice with reproducible results (P = 0.035, 0.005, and 0.035, respectively).
(A) Survival of OG1RF, TX10293, and TX37200 in BHI, pH 3.4, adjusted with lactic acid. (B) Survival of OG1RF, TX10293, and TX37200 in BHI at 55°C. The percent surviving 40 min from time zero is shown (see Materials and Methods for details).
Enterococci are reported as being able to grow in harsh environments, such as 6.5% NaCl, pH 9.6, and a wide range of temperature (10 to 45°C, with survival at 60°C for 30 min) (15); indeed, in our study, OG1RF was found to be able to survive in pH 3.4 for at least 40 min. Bacteria commonly use two-component regulatory systems to adapt to environmental challenge, and adaptation is also felt to be essential for bacterial survival and growth in mammalian hosts (9). Since expression of virulence factors can also be regulated by two-component systems during the infection, we felt that a study of the two-component systems in enterococci might provide information on their pathogenesis. In this study, genes representing seven PhoP-PhoS homologs were disrupted in E. faecalis OG1RF, and one of the mutants, TX10293 (etaR mutant), was attenuated in the mouse peritonitis model, suggesting that the EtaRS system is involved in virulence of E. faecalis.
The EtaRS system shows a high level of similarity to the two-component system LisRK of L. monocytogenes (3), and the etaRS and lisRK loci have similar gene organization. The etaR disruption mutant TX10293 was shown to be significantly more sensitive to low pH than the parent strain, OG1RF, during logarithmic growth, similar to the phenotype of the lisK deletion mutant (3). The etaR mutant (TX10293) also showed a tolerance for high temperature different from that of OG1RF. While the lisK deletion mutant showed a higher tolerance for a high ethanol concentration (5%) than the parent strain, growth of the etaR mutant (TX10293) in BHI with 5% ethanol was not significantly different from that of wild-type OG1RF (data not shown). These results suggest that the EtaRS system is involved in stress response, similar to the LisRK system in L. monocytogenes, although these systems may respond differently to different environmental cues. TX10293 showed lower resistance to low pH but higher resistance to high temperature than wild-type OG1RF, suggesting that the EtaRS system may regulate (negatively or positively) expression of different operon(s) in response to different environmental cues, or regulation of a specific operon may produce different phenotypes under different conditions. EtaRS also showed high similarity to the CsrRS system of S. pyogenes (6, 8, 12, 14). CsrRS has been shown to be a negative regulator of several virulence factors in S. pyogenes, and inactivation of CsrRS causes the strain to produce mucoid colonies. Interestingly, the E. faecalis etaR mutant (TX10293), when recovered from the mouse spleen, was mucoid on plates, while the wild-type OG1RF was nonmucoid, suggesting that the EtaRS system may negatively regulate capsule synthesis, similar to the CsrRS system. This phenotype was only observed once and disappeared quickly in vitro, suggesting that some specific condition(s) in vivo may trigger the phenotype or this phenotype may be disadvantageous in vitro.
We also found that the etaRS mutant of OG1RF was attenuated in a mouse peritonitis model, similar to a lisRK mutant of L. monocytogenes, which was attenuated in a mouse peritonitis model (3). A transposon disruption mutation in csrR reduced S. pyogenes internalization (11), and a spontaneous csrRS mutant of S. pyogenes showed enhanced virulence in a murine model of skin and soft tissue infection (4). Differences in these results may be due to different animal and tissue culture models, and/or the operons regulated by the EtaRS and CsrRS systems may not be the same. In addition, the csrRS mutant of S. pyogenes was generated spontaneously (4); thus, it was not clear whether genes other than csrRS were affected in these mutants.
In considering a possible link between the in vitro and in vivo phenotypes of the mutants, it is useful to consider some possible events in the mouse peritonitis model. It has been shown with L. monocytogenes that after bacteria are injected into the mouse peritoneum, the host immune response ensues with the arrival of neutrophils and macrophages (7). To survive this response, bacteria need to develop the ability to resist phagocytosis and/or to survive in the phagosome (21). At this stage, the sensing of and response to the in vivo environment (such as the stress conditions in the phagosome, including acidification and a wide range of lethal enzymes) are important (21), and such sensing and response could be mediated by two-component systems involved in stress response. One such example is the LisRK system of L. monocytogenes, which was initially identified as a two-component system involved in stress response (including response to low pH and high ethanol concentrations) and later was shown to be important for the virulence of L. monocytogenes. In this study, the potential two-component system EtaRS was also shown to be involved in stress response in vitro (affecting survival of E. faecalis at low pH and high temperature) and important for virulence of E. faecalis in vivo, which adds to the increasing evidence that stress responses mediated by two-component systems may regulate virulence of pathogens in vivo.
In summary, a number of potential two-component systems were identified for E. faecalis, one of which, EtaRS, was shown to be involved in response to heat and acidification in vitro and important for virulence of E. faecalis in a mouse peritonitis model. It will be of interest in future studies to look for gene(s) that are regulated by these two-component systems.
This work was supported by NIH grant AI47923 from the Division of Microbiology and Infectious Diseases to Barbara E. Murray.
We are grateful to The Institute for Genomic Research (TIGR) for providing the E. faecalis V583 genome sequence database.
Received 29 August 2001.
Returned for modification 4 December 2001.
Accepted 18 January 2002.
Editor: E. I. Tuomanen
American Society for Microbiology
Arthur, M., F. Depardieu, and P. Courvalin. 1999. Regulated interactions between partner and non-partner sensors and response regulators that control glycopeptide resistance gene expression in enterococci. Microbiology 145:1849-1858.
Chung, C. T., S. Z. Niemela, and R. H. Miller. 1989. One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proc. Natl. Acad. Sci. USA 86:2172-2175.
Cotter, P. D., N. Emerson, C. G. M. Gahan, and C. Hill. 1999. Identification and disruption of lisRK, a genetic locus encoding a two-component signal transduction system involved in stress tolerance and virulence in Listeria monocytogenes. J. Bacteriol. 181:6840-6843.
Engleberg, N. C., A. Heath, A. Miller, C. Rivera, and V. J. DiRita. 2001. Spontaneous mutations in the CsrRS two-component regulatory system of Streptococcus pyogenes result in enhanced virulence in a murine model of skin and soft tissue infection. J. Infect. Dis. 183:1043-1054.
Evers, S., and Courvalin, P. 1996. Regulation of VanB-type vancomycin resistance gene expression by the VanS(B)-VanR(B) two component regulatory system in Enterococcus faecalis V583. J. Bacteriol. 178:1302-1309.
Federle, M. J., K. S. Mciver, and J. R. Scott. 1999. A response regulator that repress transcription of several virulence operons in the group A streptococcus. J. Bacteriol. 181:3649-3657.
Gahan, C. G. M., and J. K. Collins. 1995. Non-dystrophic 129 REJ mice are susceptible to i.p. infection with Listeria monocytogenes despite an ability to recruit inflammatory neutrophils to the peritoneal cavity. Microb. Pathog. 18:355-364.
Heath, A., V. J. DiRita, N. L. Barg, and N. C. Engleberg. 1999. A two-component regulatory system, CsrR-CsrS, represses expression of three Streptococcus pyogenes virulence factors: hyaluronic acid capsule, streptolysin S, and pyrogenic exotoxin B. Infect. Immun. 67:5298-5305.
Hoch, J. A., and T. J. Silhavy. 1995. Two-component signal transduction. ASM Press, Washington, D.C.
Hulett, F. M. 1996. The signal-transduction network for Pho regulation in Bacillus subtilis. Mol. Microbiol. 19:933-939.
Jadoun, J., and S. Sela. 2000. Mutation in csrR global regulator reduces Streptococcus pyogenes internalization. Microb. Pathog. 29:311-317.
Levin, J. C., and M. R. Wessels. 1998. Identification of csrR/csrS, a genetic locus that regulates hyaluronic acid capsule synthesis in group A streptococcus. Mol. Microbiol. 30:209-219.
Li, X., G. M. Weinstock, and B. E. Murray. 1995. Generation of auxotrophic mutants of Enterococcus faecalis. J. Bacteriol. 177:6866-6873.
Miller, A. A., N. C. Engleberg, and V. J. DiRita. 2001. Repression of virulence genes by phosphorylation-dependent oligomerization of CsrR at target promoters in S. pyogenes. Mol. Microbiol. 40:976-990.
Murray, B. E. 1990. The life and times of the enterococcus. Clin. Microbiol. Rev. 3:46-65.
Murray, B. E., K. V. Singh, R P. Ross, J. D. Heath, G. M. Dunny, and G. M. Weinstock. 1993. Generation of restriction map of Enterococcus faecalis OG1 and investigation of growth requirements and regions encoding biosynthetic function. J. Bacteriol. 175:5216-5223.
Qin, X., K. V. Singh, Y. Xu, G. M. Weinstock, and B. E. Murray. 1998. Effect of disruption of a gene encoding an autolysin of Enterococcus faecalis OG1RF. Antimicrob. Agents Chemother. 42:2882-2888.
Reed, L. J., and H. Muench. 1938. A simple method of estimating fifty percent endpoints. Am. J. Hyg. 27:493-497.
Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
Singh, K. V., X. Qin, G. M. Weinstock, and B. E. Murray. 1998. Generation and testing of mutants of Enterococcus faecalis in a mouse peritonitis model. J. Infect. Dis. 178:1416-1420.
Small, P. L. C., L. Ramakrishanan, and S. Falkow. 1994. Remodeling schemes of intracellular pathogens. Science 263:637-639.
Xu, Y., K. V. Singh, X. Qin, B. E. Murray, and G. M. Weinstock. 2000. Analysis of a gene cluster of Enterococcus faecalis involved in polysaccharide biosynthesis. Infect. Immun. 68:815-823.
Infection and Immunity Apr 2002, 70 (4) 1991-1996; DOI: 10.1128/IAI.70.4.1991-1996.2002
You are going to email the following Involvement of PhoP-PhoS Homologs in Enterococcus faecalis Virulence


text:
Home / News / Meroncourt moves into new areas
UK distributor widens product portfolio with the latest in a spate of European signings
Meroncourt moves into new areas
9th September 2009	News
Distributor Meroncourt is moving into a number of new product categories, including DVD and digital music players, following a deal with Spanish vendor Energy Sistem Soyntec – the latest in a slew of European product lines that the company has introduced to the UK recently.
Soyntec has established itself on the continent as a market leader in digital consumer electronics and peripherals, with a focus on design that is common to many European manufacturers. As well as its media portfolio, the firm also produces PC accessories and peripherals.
"This was a conscious decision," sales director Steve Walsh told PCR, when asked about Meroncourt's recent spate of European signings. "We're always looking to broaden our product base and bring new ideas in to the UK. Soyntec has some very funky designs, which coupled with the quality of their products gives us an edge. This is certainly a new horizon for us and we've been very impressed so far."
Meroncourt has also revamped its online presence, and the site can now integrate XML
feeds. This means partners can now download product information and images directly from the Meroncourt website for incorporation in to their own – a service that had previously only been available on request.
Tags awoodennbmedia-com meroncourt
Previous Microsoft attacks Google book deal
Next Orange and T-Mobile set sights on iPhone
The future according to the tech industry elite
Wired for sound: How retailers can sell more audio products
Stone to offer Lenovo servers to education and public sector
Smithie UK on the hunt for experienced storage salesperson
Distributor Smithie UK has announced that it is on the hunt for an experienced storage …

redpajama_set_name: RedPajamaCommonCrawl


Adrian Johns is professor of history and chair of the Committee on Conceptual and Historical Studies of Science at the University of Chicago. He is the author of The Nature of the Book: Print and Knowledge in the Making, also published by the University of Chicago Press.
"In his invaluable book Piracy, Adrian Johns argues that the tendency of intellectual property battles to undermine privacy is not new. On the contrary, Johns . . . argues that ever since the medieval and Enlightenment eras, corporations have tried to defend their economic interests by searching for intellectual piracy in the private sphere of people's homes. He says that all of our current debates about intellectual piracy—from Google's efforts to create a universal digital library to the fight over how vigorous patents should be—have antecedents in the copyright wars of earlier eras."


text:
Waverly, Iowa
Moving to Waverly, Iowa is a big decision
Should you move to Waverly, Iowa? Is Waverly,Iowa a good place to live? When determining what cities are good for your personality type, life situation, and personal preferences, there are many factors to consider including climate, cost of living, community, job opportunities, population makeup, activities, and things to do.
With data on over 50,000 cities in the United States, Dwellics makes it easy to find the best place for you to move based on your personal preferences. This page contains detailed facts and statistics to help you determine if moving to Waverly,Iowa is the right decision for you.
Cost of living in Waverly, Iowa
Is it expensive to live in Waverly, Iowa? When calculating the cost of living in Waverly, Iowa we factor in state income tax rates, average housing rental prices, average property values, cost of living index, and more.
The cost of living in Waverly, Iowa is moderateAnalytics team at Dwellics grouped locations into 5 ranges for each metric: Lowest, Low, Moderate, High, Highest in the United States and lowAnalytics team at Dwellics grouped locations into 5 ranges for each metric: Lowest, Low, Moderate, High, Highest in Iowa, while median household income in Waverly is $64,949 which is high Analytics team at Dwellics grouped locations into 5 ranges for each metric: Lowest, Low, Moderate, High, Highest in the United States and highAnalytics team at Dwellics grouped locations into 5 ranges for each metric: Lowest, Low, Moderate, High, Highest in Iowa.
Taxes in Waverly, Iowa
The sales tax rate in Waverly, Iowa is 7%. Last year, the average property taxes paid was 0.9% Waverly,Iowa.
Here is the table with the State Income Taxes in the state of Iowa:
Iowa Income Tax Rate
$0 $1,638 0.33%
$1,638 $3,276 0.67%
$6,552 $14,742 4.14%
$14,742 $24,570 5.63%
Housing costs in Waverly, Iowa
In Waverly, Iowa, 77.7% of the population owns their homes, while approximately 22.3% rent. The average cost of a single-family home in Waverly, Iowa in 2021 is $193,661 which is highAnalytics team at Dwellics grouped locations into 5 ranges for each metric: Lowest, Low, Moderate, High, Highest in the United States and the highestAnalytics team at Dwellics grouped locations into 5 ranges for each metric: Lowest, Low, Moderate, High, Highest in Iowa. The average one-bedroom apartment cost is approximately $600 per month.
Compared to Iowa
1 Bedroom $600 highAnalytics team at Dwellics grouped locations into 5 ranges for each metric: Lowest, Low, Moderate, High, Highest
Climate in Waverly, Iowa
What is the climate like in Waverly, Iowa? There are many important factors that may affect your personal climate preferences including average temperature and humidity, annual rainfall and snow, number of cloudy days, wind speeds, and more.
Climate comfort in Waverly, Iowa
In a typical year, Waverly, Iowa temperatures fall below 50F&deg for 177 days per year. Annual precipitation in Waverly is typically 34.5 inches per year (lowAnalytics team at Dwellics grouped locations into 5 ranges for each metric: Lowest, Low, Moderate, High, Highest in the US) and snow covers the ground 75 days per year or 20.5% of the year (highAnalytics team at Dwellics grouped locations into 5 ranges for each metric: Lowest, Low, Moderate, High, Highest in the US). It may be helpful to understand the yearly precipitation by imagining 7 straight days of moderate rain per year. The humidity in Waverly, Iowa is below 60% for approximately 163 days or44.7% of the year.
Sunrise and sunset times vary by region in the United States. The duration of each day and the amount of sunlight can affect people's moods in a significant way. In Waverly,Iowa, the shortest day starts at 07:43 a.m. and ends at 04:35 p.m. and lasts for 9 hours 01 minutes hours. The longest day starts at 05:31 a.m. and ends at 08:51 p.m. and lasts for 15 hours 19 minutes hours.
Is Waverly, Iowa a safe place to live?
Dwellics analytics team assigned the score 68.8 to Waverly, Iowa (with 100 being the best). This score helps find the safest places to live in America. When determining if Waverly, Iowa is a safe place to live, we analyze factors including air quality, water quality, crime rates, natural disaster frequency, substance abuse rates, cancer instances, and more.
Crime rates in Waverly, Iowa
In Waverly, Iowa, violent crime rates are considered to be highAnalytics team at Dwellics grouped locations into 5 ranges for each metric: Lowest, Low, Moderate, High, Highest in the United States according to our research. Arson crimes are lowAnalytics team at Dwellics grouped locations into 5 ranges for each metric: Lowest, Low, Moderate, High, Highest and property crimes, including vandalism rank moderateAnalytics team at Dwellics grouped locations into 5 ranges for each metric: Lowest, Low, Moderate, High, Highest.
Environmental Hazards Waverly, Iowa
In Waverly, Iowa, cancer instances are considered to be moderateAnalytics team at Dwellics grouped locations into 5 ranges for each metric: Lowest, Low, Moderate, High, Highest in the United States according to our research. Substance abuse in Waverly, Iowa is the highestAnalytics team at Dwellics grouped locations into 5 ranges for each metric: Lowest, Low, Moderate, High, Highest in the United States according to our research.
Natural Disasters in Waverly, Iowa
Earthquakes Earthquakes in Waverlyare unlikely
Hurricanes Hurricanes inWaverly are unlikely
Severe Storms Severe Storms inWaverly are high risk
Tornados Tornadoes in Waverlyare possible
Volcanoes Volcanic hazard in Waverly is unlikely
Wildfires Wildfires in Waverlyare very low
Smoking Laws in Waverly, Iowa
Smoking is prohibited inside restaurants and bars in Waverly. Smoking inside of workplaces in Waverly is prohibited. The use of e-cigarettes is banned. In Waverly, Iowa marijuana use is not legal.
Air & Water Quality in Waverly, Iowa
Waverly, Iowa has good air quality 95% of the time or 346 days per year. The water quality index calculated by Dwellics team in Waverly is 100 out of 100 (100 being the best) based on the number of acute health-based violations for every water utility in the last 5 years.
Does Waverly, Iowa have good schools?
When determining if Waverly, Iowa, has good schools, we analyze key factors including math proficiency, reading/language proficiency, students-per-teacher ratio, average teacher salaries, National Blue Ribbon awards, Spelling Bee and STEM/non-STEM national competitions, and more.
Elementary schools in Waverly Rank #68.4Dwellics analytics team assigned the score 68.4 to elementary education in Waverly, Iowa (with 100 being the best). in the country. 27.5%
Middle schools in Waverly Rank #67.4Dwellics analytics team assigned the score 67.4 to middle education in Waverly, Iowa (with 100 being the best). in the country. 5.7%
High schools in Waverly Rank #67.2Dwellics analytics team assigned the score [r_high] to high education in Waverly, Iowa (with 100 being the best). in the country. 2.4%
Dwellics searched for winners of 69 nationwide competitions for school children in both STEM and non-STEM fields and found 0 winners in the Waverly, Iowain the last 5 years.
Elementary Schools in Waverly, Iowa
There are 3 public elementary schools in Waverly, Iowa. The public elementary schools inWaverly are ranked #68.4 (with 100 being the best) in the United States according to our research. Among elementary school students, approximately 27.5% are enrolled at private schools in Waverly.
The average percentage of students proficient in math in Waverly, Iowa at the elementary level is 83.2%,and proficient in reading & language arts 75.6%.
The average salary of an elementary school teacher in Waverly is $44,304 per year. The average number of elementary school students assigned to a teacher's classroom in Waverly is 15.
Middle Schools in Waverly, Iowa
There are 1 public middle schools inWaverly, Iowa. The public middle schools in Waverly are ranked #67.4 (with 100 being the best) in the United States according to our research. Among middle school students, approximately 5.7% are enrolled at private schools in Waverly.
The average percentage of students proficient in math in Waverly, Iowa at the elementary level is 81%, and proficient in reading & language arts 71%.
The average salary of a middle school teacher in Waverly is $44,304 per year. The average number of middle school students assigned to a teacher's classroom in Waverly is 14.
High Schools in Waverly, Iowa
There are 2 high schools in Waverly, Iowa. The public high schools in Waverly are ranked #67.2 (with 100 being the best) in the United States according to our research. Among high school students, approximately 2.4% are enrolled at private schools in Waverly.
The average percentage of students proficient in math in Waverly, Iowa at the elementary level is 77.6%, and proficient in reading & language arts 82.5%.
The average salary of a high school teacher in Waverly is $44,304 per year. The average number of high school students assigned to a teacher's classroom in Waverly is 15.
Infrastructure and Government in Waverly, Iowa
Waverly, Iowa is a small suburb in a metro area in the Central Time Zone.The wireless internet speeds inWaverly are approximately 61 Mbps, while wired internet connections average a speed of 145 Mbps.
Transportation in Waverly, Iowa
The nearest airport to Waverly is 13 miles away. The nearest coastline is 219 miles away. Compared to national averages, traffic on highways and roads in Waverly is the lowestAnalytics team at Dwellics grouped locations into 5 ranges for each metric: Lowest, Low, Moderate, High, Highest. The road network is moderateAnalytics team at Dwellics grouped locations into 5 ranges for each metric: Lowest, Low, Moderate, High, Highest.
Community Demographics in Waverly, Iowa
In terms of political views, the Waverly population skews Republican. The top religion in Waverly is Protestant. The top age group is 45-64 per census data.
Household Types in Waverly, Iowa
Singles in Waverly 51.1%
Married Couples in Waverly 48.9%
Age Groups of People Living in Waverly, Iowa
Race Diversity in Waverly, Iowa
The Race Diversity score in Waverly, Iowa is 9.Race diversity score ranges from 0 to 100. The more diverse, the higher the score..
Black or African Americans 2%
The Religion of people living in Waverly, Iowa
The primary religious affiliation in Waverly, Iowa is Protestant. The Religion Diversity score inWaverly, Iowa is 12.Religion diversity score ranges from 0 to 100. The more diverse, the higher the score..
Industry and Occupation in Waverly, Iowa
The top industry present in Waverly, Iowa is Educational services, health care, and social assistance. 37.3 % of residents work in Management, business, science, and arts occupations, while only 7.1% work in Natural resources, construction, and maintenance occupations. The Industry Diversity score in Waverly, Iowa is 55.8.Industry diversity score ranges from 0 to 100. The more diverse, the higher the score.
Manufacturing 15.4%
Other services, except public administration 4%
The Occupation Diversity score in Waverly, Iowa is 78.Occupation diversity score ranges from 0 to 100. The more diverse, the higher the score.
Average Educational level of people living in Waverly,Iowa
69.1% of residents in Waverly, Iowa have earned a Bachelor's degree or higher, compared to the U.S. average of 46.6%. 37.6% of residents in Waverly, Iowa hold STEM degrees (STEM = Science, Technology, Engineering, Mathematics). The Educational level Diversity score in Waverly, Iowa is 74.2.Educational diversity score ranges from 0 to 100. The more diverse, the higher the score.
Less than high school graduate 3%


One of the world's most talented mandolin players is coming to Newtown just in time for the Valentine's Day weekend. Sierra Hull will be bringing her bluegrass music and eight stringed instrument to the Zlock Performing Arts Center on Friday, Feb. 15.
Sierra Hull named 2016 Mandolin Player of the Year. Photo by Dave Brainard.
The evening promises Hull's full ensemble and her Southern-forged musical prowess. As a singer and songwriter with several studio albums, Hull has proven herself as a talented artist. A sample of her music is available here.
Hull grew up with her family in Tennessee where music permeated her existence. Singing in church, along with almost every relative, was just one of the facets in which music became a way of life.
Her father introduced her to the mandolin when she was eight years old. It was the perfect size for a small child who had earlier received a large fiddle for Christmas.
Just two years later she debuted at the Grand Ole Opry, then played at Carnegie Hall at age 12. She has since gone on to study at Berklee, play at the White House, and win Mandolin Player of the Year with the International Bluegrass Music Association Awards three years in a row starting in 2016. To top it off, Sierra Hull is only 27 years old.
Hull will be playing with her ensemble at the auditorium on Bucks County Community College campus on Friday, Feb. 15. The performance begins at 7:30 p.m. and tickets are available here.


text:
Source: Sid Gentle-BBC
Emmys spotlight: Fiona Shaw on the secrets of her 'Killing Eve' spymaster
As a lockdown phone conversation makes clear, Fiona Shaw is nothing like Carolyn Martens, the mysterious, often arrogant MI6 spymaster she plays with irresistible aplomb in Killing Eve.
For one thing, Shaw talks with a soft Irish accent that is in marked contrast to her character's clipped upper-class English tones. "I had learned that people who are secretive tend to pause before they speak," says the experienced stage and screen actress, whose performance in the second season of the witty thriller series earned her a 2019 Emmy nomination and the best supporting actress TV Bafta.
"So I began to pause and pausing made me stiller, and by being stiller I began to think I knew a lot more than anybody else did in this story. That early thing of stopping her brain from gushing – in the way you may be hearing me doing to you now – is Carolyn."
In the series, originally developed for TV by Phoebe Waller-Bridge (and produced by Sid Gentle Films for BBC America, with Endeavor Content distributing), it is Carolyn who recruits intelligence investigator Eve (played by Sandra Oh) to track down psychopathic assassin Villanelle (Jodie Comer). As lead writer for season one, Waller-Bridge "quickly fell for Carolyn", Shaw recalls, finding different sides to the stiff British spy.
"And that's just been extended and expanded by other writers as it's gone along," says Shaw. "The great pleasure of playing in Killing Eve is that there's much more contradiction in the characters than you normally get to play. That doesn't mean they don't add up, but that they are as interesting as we are as people."
Still, she adds: "If you think that I've solved the character, I really haven't. I'm hoping more surprises are around the corner."
There were certainly surprises in the series' recently concluded third season (headed by Suzanne Heathcote as lead writer) as Carolyn faced the death of someone close to her and was pushed into uncharacteristically violent action. The season was "a lot less 'fun', in the sense of fun", Shaw admits.
"It tickles the writers' fancies to say what would happen if [Carolyn's] cool, calm exterior gets threatened. And I was very challenged by that. I was sympathetic to her repressiveness, and I worked hard to try and catch it."
There may be more surprises ahead in the fourth season, which according to the show's usual schedule should start its six-month shoot this autumn – a situation that may have to be reconsidered because of the pandemic and its knock-on effects.
Lead writer for the fourth season is Laura Neal (whose credits include Netflix's comedy-drama Sex Education) and, without revealing any secrets, Shaw confirms that, as she and other cast members have done with lead writers in previous years, she has talked to Neal about what is in store for Carolyn Martens – and offered a few suggestions of her own.
"It's good for us to talk to the writers," Shaw says, because "it's the underlying identity of the character that you're trying to get to. Otherwise it's not alive, it's just a cardboard cutout. And the longer we play them, of course, the more possessive all the actors are of the characters.
"But equally I think the fun of the experience is that the writer should write something outlandish that throws a curveball at the character, so they're not allowed to sit in their comfort zone."
Source: BBC America/Parisa Taghizadeh
'Killing Eve', Sandra Oh and Fiona Shaw
Starring on one show for three seasons has been a new experience for Shaw, whose previous TV work has consisted mainly of guest spots, occasional TV movies and miniseries – the BBC's Mrs Wilson being the latest – and a 12-episode run nearly a decade ago in HBO fantasy drama True Blood.
During each of Killing Eve's first two seasons, Shaw pursued a new career path directing opera productions – Medea for Wexford Festival Opera and Cendrillon for Glyndebourne – but she gave up that sideline when the series was renewed for its third season.
"I knew then that this was taking over my life," she says. "But it has very happily taken over my life because with the episodic nature of our work – whether one's performing in a play or in a series or traveling – it has been marvellous to know what I'm doing for those six months [each year]."
Killing Eve is just the latest critical and commercial success for Shaw. On stage in London and New York she has made award-winning appearances in As You Like It, Hedda Gabler, The Taming Of The Shrew, Machinal and Electra, among others.
Over her 30 years working in film, meanwhile, she has taken in 1990s Hollywood comedies such as Three Men And A Little Lady and Super Mario Bros through auteur dramas including Jim Sheridan's My Left Foot, Franco Zeffirelli's Jane Eyre and Terrence Malick's The Tree Of Life to five of the Harry Potter films, in which she played the young wizard's disagreeable Aunt Petunia.
And between seasons two and three of Killing Eve, she added another three – yet to be released – features to that list: independent UK thriller Corvidae; Netflix's young-adult mystery Enola Holmes, with Millie Bobby Brown starring and Killing Eve's Harry Bradbeer directing; and writer/director Francis Lee's period drama Ammonite, starring Saoirse Ronan and Kate Winslet.
Ammonite was included in the Newcomers section of this year's Cannes Official Selection. If the coronavirus had not put paid to the physical event, Shaw, whose brother lives in the south of France, "would have loved to have been with him, swanning around the Croissette".
And just for good measure, after Killing Eve's third season wrapped in January, Shaw began work in Budapest on Baptiste, joining the BBC crime drama with Tcheky Karyo for its second season until the pandemic halted production mid-shoot.
There have been plenty of offers, thanks in part to the zeitgeist-tapping success of Killing Eve. "I have had a lovely year of things coming in the post," says London and New York-based Shaw, who says she would be particularly keen for a US-shot project to bring her back to the States. But with one-and-a-half TV series pending and concerns about post-virus shooting conditions, Shaw, like many in the industry, is looking to the future with a certain amount of caution.
"I'm not going to say yes to anything until after next February," she says. "I think it will take the autumn, if we're lucky, to get back to Baptiste. Then there's Killing Eve over the winter, if we're lucky. I wouldn't have thought I'd be taking any chances on anything that's hoping to shoot before then."
Screen Emmys 2020: digital edition
UK & Europe Newsletter


text:
Microsoft Discontinues Xbox Fitness Service
By: John Quilty
Microsoft has announced that it will be cutting Xbox Fitness. The service was an exclusive brand under which Microsoft sold various fitness videos from popular fitness figures such as Jillian Michaels and Tony Horton. As opposed to simply playing back videos, it would utilize the Kinect to track heart rate, sweat, balance, and estimate calories burned. However, after the post-announcement backlash that the Kinect would be required for the Xbox One to run at all, Microsoft later revamped Fitness to not require the Kinect.
In its announcement, Microsoft noted that all sales will cease immediately. Any purchased media will be available until June 30, 2017, and it will shut down entirely July 30, 2017.
Beginning today, June 27, 2016, Xbox Fitness content will no longer be available for purchase. For existing users of Xbox Fitness, the content you've purchased will be available to play for a year, through June 30, 2017.
On December 15, 2016, we will be removing our "Free with Gold" offer. Until then, Gold members will still be able to access and play the over 30+ workouts available for streaming as a part of this offer for free.
On July 1, 2017, Xbox Fitness (and all associated content) will no longer be available for download/play. This includes content you've purchased. At that time, Xbox Fitness will no longer be available for download from Xbox Marketplace and content will not be available for play from the Xbox Marketplace nor within the game. Xbox Fitness users will no longer be able access Xbox Fitness and the associated workouts/programs.
Xbox Fitness' data was capable of integrating into Microsoft's Health tracking system, which is also used with the Microsoft Band, their smartwatch/activity tracker. At this time, Microsoft has not announced any plans to change or discontinue the Health service altogether.
Did you use Xbox Fitness? Are you sad to see it go? What do you think led to this decision? Let us know in the comments.
John Quilty
I've been a lover of video games, writing, and technology for as long as I remember. I have a B.A. in English from the University of Illinois at Urbana-Champaign and I'm happy to write about gaming and technology for TechRaptor.


text:
Brunos garvaregård utgörs av två byggnader i kvarteret Korpen vid Drottninggatan 24/Norra Ringgatan 25 i Alingsås. Byggnaderna, som uppfördes under 1700-talet och 1980-talet, är byggnadsminne sedan den 31 januari 1983.

Historia
Kvarteret Korpen indelades och bebyggdes under 1700-talets förra hälft och hade ursprungligen en kvadratisk form, indelat i fyra långsträckta och genomgående tomter efter medeltida mönster. Under 1760-talet och i samband med J. Forsells stadsplaneförslag fick kvarteret sin rektangulära och aktuella sträckning. Av TH Wästfeldts planförslag från 1878 framgår att bebyggelsens placering inom tomterna i stort överensstämmer med befintlig struktur. Kvarterets funktioner har sedan 1700-talet dominerats av olika hantverk, till exempel sämskmakare och gullslagare, vilket återspeglas i byggnadernas och gårdarnas utformning. Den karaktäristiska och funktionsdifferentierade hantverksgården från 1700-talet finns idag dokumenterad i Brunos garvaregård, tomt nr 2, vilken tillsammans med de äldre gatuhusen på tomterna 1, 2, 3 och 5 bildar en sammanhållen och tidstypisk miljö från 1700–1800-talen med avseende på tidigare funktion och formspråk.

Beskrivning
Den karaktäristiska och funktionsdifferentierade hantverksgården från 1700-talet representeras idag av byggnaderna i fastigheten Korpen 2. Bostadshuset som uppfördes vid 1800-talets början vänder sig med en gråmålad fasad mot gatan och med faluröd fasad mot gården. Gårdsytan är täckt med kullersten och gatsten. Byggnaderna är av varierande ålder, varav den västra ekonomibyggnaden är uppförd på platsen av en tidigare byggnad efter beslutet om byggnadsminnesförklaring. Bostadshuset och ekonomibyggnaderna bildar en sluten gårdsenhet.

Bostadshuset är en timrad byggnad i två våningar. Byggnaden vänder sig med en gråmålad fasad med locklistpanel mot Drottninggatan. Fasaden har utskjutande knutar. Sadeltaket är täckt med enkupigt tegel. Bottenvåningen har fönster som är spröjsade i fyra rutor medan övervåningen har höga fönster med överluftsfönster. Bågarna är målade i en rödbrun kulör. Väster om huset finns ett träplank som är sammanbyggt med grannfastigheten. Mot gårdsplanen är fasaden klädd med faluröd locklistpanel. Fönstren är småspröjsade eller är indelade i fyra respektive sex rutor. Mot gården finns även balkong med vita snickerier.

På platsen för nuvarande ekonomibyggnad har stått en byggnad som revs före byggnadsminnesbeslutet. Nuvarande byggnad är uppförd på 1980/1990-talet. Sadeltaket är täckt med enkupigt tegel. Fasaden är klädd med faluröd locklistpanel. Ytterdörren har ett överljusfönster. Fönstren har två lufter som vardera är spröjsad i sex rutor.

Referenser

Vidare läsning

Externa länkar

Byggnader i Alingsås kommun
Byggnadsminnen i Västra Götalands län
Alingsås historia
Byggnader invigda under 1700-talet

redpajama_set_name: RedPajamaWikipedia


text:
Press Release: News advance 4/21 – Data Gumbo nabs energy veteran as Chief Corporate Development Officer
April 19, 2021 Richard Carranza Uncategorized Leave a comment
HOUSTON, TEXAS — April 21, 2021 — Data Gumbo, provider of GumboNet™ — the massively interconnected industrial smart contract network secured and powered by blockchain, today announced that it expanded its executive team with the addition of Robin Macmillan as Chief Corporate Development Officer to lead the Corporate Development team.
Macmillan joins Data Gumbo with a 40+ year career in energy with the last 12 years as Vice President and Senior Vice President for Business Development at National Oilwell Varco (NOV Inc.), the American multinational corporation and leading worldwide provider of equipment and services to upstream the oil and gas industry.
"The sheer breadth of Macmillan's experience will serve as an invaluable asset to Data Gumbo as we continue to exponentially grow and mature our company into new industrial markets and further solidify our leadership in energy," said Andrew Bruce, CEO and Founder, Data Gumbo. "Macmillan has the experience to expand Data Gumbo's commercial market penetration to aid companies in undertaking digital transformation with smart contracts to reveal streamlined efficiencies and cost savings, sustainability insights across supply chains and transactional certainty in any commercial relationship."
Macmillan is the current VP of Drilling Services at the International Association of Drilling Contractors (IADC) where he is also Chair Emeritus of the Advanced Rig Technology committee. He is also vice-chair of the Society of Petroleum Engineers (SPE) Drilling Systems Automation Technical Section. With a degree in Geology from the University of Leeds in England, Macmillan has worked in numerous countries including eight years in Latin America. His previous experience includes Management of Schlumberger Drilling and Measurements in Canada and President at the drill bit company ReedHycalog.
"There is tremendous opportunity right now to change how business is executed," said Macmillan. "Data Gumbo is poised to deliver trust through automated, auditable blockchain-backed smart contracts that execute transactions in real-time. I am thrilled to be a part of the Data Gumbo executive team as the company is in a period of hyper growth into new industries, serving as a harbinger for significant digital transformation across commercial relationships and transparent, accurate sustainability impact data."
Previous Post: Richard Carranza, C-SPAN Open Phones, Immigration 4/19/2021
Next Post: Democratic Controlled House: Living In A House Of Inequity


The ten year study course offered at SHIC is divided into four levels: five year course in secondary level, two year course in senior secondary level and three year course in degree level. The syllabus contains all relevant Islamic and modern subjects like Quran, Hadith, Fiqh, Tafsir, Tasawwuf, Nahw, Aqidah, Mantiq, Islamic history, Arabic literature, Rhetoric and prosody, Translation and Composition, History, Science and Technology, Mathematics, social sciences, Computer and Information Technology and etc. Enough attention is paid to make the students well acquainted with four languages, namely Arabic, English, Urdu and Malayalam. SHIC curriculum makes mandatory for every undergraduate student to pursue an official degree course in any of the Arts or Humanities subjects utilizing the open degree system offered by many universities.


text:
EU businesses can use IOSS portal from Jul 1 for VAT e-com obligations
July 1, 2021 by Yasar Hanif
Businesses in the European Union (EU) can use the Import One-Stop Shop (IOSS) electronic portal from July 1 to comply with their value-added tax (VAT) e-commerce obligations on distance sales of imported goods. The earlier VAT rules, under which no import VAT was required for commercial goods of value up to €22, will come to an end from today.
So beginning today, all commercial goods imported into the EU from a third country or third territory will be subject to VAT irrespective of their value.
The IOSS allows suppliers and electronic interfaces selling imported goods to buyers in the EU to collect, declare and pay the VAT to the tax authorities, instead of making the buyer pay the VAT at the moment the goods are imported into the EU as it was previously the case (for products over €22).
The IOSS facilitates the collection, declaration and payment of VAT for sellers that are making distance sales of imported goods to buyers in the EU. The IOSS also makes the process easier for the buyer, who is only charged at the time of purchase, and therefore, does not face any surprise fees when the goods are delivered, the EU said on its website.
If the seller is not registered in the IOSS, the buyer has to pay the VAT and usually a customs clearance fee charged by the transporter.
Businesses have been allowed to register on the IOSS portal of any EU member state from April 1 this year. If businesses are not based in the EU, they will normally need to appoint an EU-established intermediary to fulfil their VAT obligations under IOSS.


Serpent 1/10 scale pancar S100 Link-Tube The 1/10 World GT version of the succesfull S120 1/12 scale pan-car in Link-Tube format. High performance 1/10 scale pan chassis, with nicely machined carbon fibre and aluminium parts. Superb fully adjustable front end creates loads of steering. Spring loaded Link system, in combination with diagonal positioned main shock and tubes left and right, control the roll of the car. Well balanced chassis offers wide selection of set-up features and options to dial the car in best for any track. No wheels/tyres, electronics or body included.
Serpent 1/10 scale pancar S100 Link. The 1/10 World GT version of the succesfull S120 1/12 scale pan-car. High performance 1/10 scale pan chassis, with nicely machined carbon fibre and aluminium parts. Superb fully adjustable front end creates loads of steering. Spring loaded Link system, in combination with diagonal positioned main shock and lateral damper with through shaft, control the roll of the car. Well balanced chassis offers wide selection of set-up features and options to dial the car in best for any track. No wheel/tyres, electronics or body included. Available start august 2010.


Volunteers and community supporters are an INTEGRAL part of our operation. We are currently looking for game day operations and community support volunteers. If you are interested in joining our family and helping it grow, complete the following application.


text:
DEF LEPPARD's JOE ELLIOTT: 'Playing Live Is, At This Moment In Time, More Important Than New Music'
During a recent appearance on the "Appetite For Distortion" podcast, Joe Elliott spoke about DEF LEPPARD's plans to record the follow-up to 2015's self-titled album. He said (hear audio below): "It's really important, I think, to keep making new music. But with us — DEF LEPPARD, I mean — we know we'll make new music. We're constantly writing, but we're not in a rush to put anything out just because we have a record deal and we have to put product out there. I think it's more important that DEF LEPPARD just be DEF LEPPARD, and that means playing live in front of people. And maybe once every five years we put a new record out, because that scratches a big enough itch for us these days more so than, say, not touring. I mean, that would be heartbreaking for us."
He continued: "The reason everybody wants to be in a band is to play live — not to spend hours and years in the studio. It wasn't anybody's dream to spend five years in the studio. But, you know, to play in front of an audience in stadiums, that kind of thing, that's the ultimate goal for a band like us. So playing live is, at this moment in time, more important than new music, but we haven't dissed new music at all. We've actually currently got a bunch of songs on the go, but we're just not in a rush to record 'em. We want 'em to be good, not quick."
This past spring, DEF LEPPARD guitarist Phil Collen says that the band had a "bunch of songs" in the works for its 12th studio album, which the band began working on last year.
DEF LEPPARD's second Las Vegas residency took place this past summer. The band took over Zappos Theater at Planet Hollywood Resort & Casino for 12 nights between August 14 and September 7.
The British rockers played their first Las Vegas residency six years ago at the Joint at the Hard Rock Hotel.
DEF LEPPARD's latest greatest-hits collection, titled "The Story So Far - The Best Of", was released last November.
QUIET RIOT Will 'Definitely' Release New Music, Says Guitarist ALEX GROSSI
DEE SNIDER On His Next Solo Album: 'Creative Juices Are Flowing And Inspiration Is There'
SKID ROW's RACHEL BOLAN Pays Tribute To NEW YORK DOLLS Guitarist SYLVAIN SYLVAIN
Hear Snippet Of New PAPA ROACH Song 'Stand Up'
Ex-GUNS N' ROSES Guitarist GILBY CLARKE Clarifies His Non-Involvement In 'Not In This Lifetime' Tour


text:
HomeGovernment
Lynne Minion | 23 Jun 2017
The South Australian Government has announced $528 million for a Women's Hospital for the Adelaide CBD in this week's State Budget, a part of what it's described as a $1.1 billion investment into the South Australian health system in 2017-18.
Providing an extra 31 beds for women and newborns, up from 117 at the existing Women's and Children's Hospital in North Adelaide, the Women's Hospital will provide services for gynaecological, maternity, mental health and neonatal patients and a "significantly larger" neonatal intensive care unit.
It's a repurposing of a 2013 pledge to build a new Women's and Children's Hospital at the site adjoining the Royal Adelaide Hospital, with a new Children's Hospital now carved off and delayed while a site is identified at the Adelaide Biomedical Precinct by the end of 2019.
Health Minister Jack Snelling said the location of the new Women's Hospital to be opened by 2024 will provide patients with access to acute-care facilities.
"Our doctors say this model will be of great benefit, in particular in situations where a mother may experience a difficult birth and require acute intensive care at the new Royal Adelaide Hospital," Snelling said.
"Having access to the new Royal Adelaide Hospital's helipad will also significantly improve the timeliness of access to air services for high-risk maternal and neonate emergencies."
However, AMA SA president William Tam said the group had lobbied the government for women's and children's services to remain together.
"This was a commitment to the community that it would co-locate both the women's and children's services near the new RAH," Tan told the Adelaide Advertiser.
"It's a shame, really, to see the separation occur and a return to the past.
"The other concern we have is that now that we have this separation, it has the potential to disconnect the clinicians who have to look after both paediatric patients and neonates."
The State Budget also includes an additional $24 million over two years towards the $64.4 million upgrade to the existing Women's and Children's Hospital.
Following the Oakden scandal, which saw about a dozen staff suspended following the chief psychiatrist's damning report into patient care at the Older Persons Mental Health facility in the Adelaide suburb, a new $13.7 million facility will be funded in the budget for people assessed with the most severe forms of behavioural and psychological symptoms of dementia.
The Queen Elizabeth Hospital will gain a new emergency department, operating theatre, day surgery suite, outpatient and medical imaging services, and brain and spinal injury rehabilitation facilities as part of a $250 million investment towards stage three of the hospital's redevelopment.
A new, larger $52.5-million emergency department will be built at the Lyell McEwin Hospital, Modbury Hospital will gain a $9.2 million eight bed Emergency Extended Care Unit, and two new operating theatres are to be built at the Flinders Medical Centre at a cost of $3.5 million.
An allocation of $44 million will go towards a second South Australian Health and Medical Research Institute – SAHMRI II – to house the Southern Hemisphere's first proton-therapy
research centre.
A $4.7 million contribution over two years will also be made to the Australian Digital Health Agency's national digital healthcare program as part of the budget.
TAGS: South Australian government, SA Health, SA Budget, Women's Hospital, healthcare IT


Since the year of its founding in 2002, Slide has been a leading company of outdoor and indoor decoration, lighting design for contract, events and residential areas. Its success is due to its founder, Giò Colonna Romano: his fancy fantasy matches perfectly with his thirty-year know-how in the processing of plastic resins. As a demiurge, Slide creates colourful design objects: starting from a humble material as polyethylene, Slide elevates it to refine high quality design and elegant art objects. The present and the future of Slide is the constantly research for innovation. Slide is always looking for new materials, as the introduction of soft and rigid polyurethane, and it is looking for new shapes, thanks to the collaboration with some of the most famous designers of the world, as Alessandro Mendini, Paola Navone, Stefano Giovannoni, Marcel Wanders, Karim Rashid and Marc Sadler. The first success was the production of luminous bar furniture, which can be the protagonists of unforgettable events and contract that do not want to be unnoticed. Slide constantly grows up in different products and sectors: we acquire and develop the Design of Love collection, we create rental service of furniture, and we look for beauty, with the Slide Art branch. Between artisanship and innovation, Slide finds its first home in Buccinasco, where offices and factory are settled, but today Slide conquers also the heart of Milan, thanks to its new opening show room in Foro Buonaparte. Today Slide is known worldwide and its design products are exported in more than 130 different countries.
Slide's main goal is to bring quality, creativity and design culture to Made in Italy all over the world. Slide takes the concept of Made in Italy a step further, thanks to the idea of "Made in Slide": we make the whole process of production on our own. Engineering, moulding, finishing, assembly and lacquering are strictly executed in our factories in Buccinasco, near Milan. Thanks to this philosophy, we gain a double advantage: on one side, we have the full knowledge of the ideational and productive process, and, on the other side, we have the absolute control over the quality of the results. Another important element of the Made in Slide philosophy is the supervision of the materials we use: low-density linear polyethylene is 100% recyclable and processed according to a zero-emission process, with a view to environmental sustainability that sees us close to Non-profit associations that fight for the protection of our planet.
NEED MORE SLIDE INFORMATION ?


Falke was founded in 1994. Since the beginning our company executives have been active as consultants and investors, continuously creating a set of connections which spans over three continents and links us to a great source of corporate contacts and advanced expertise. Falke international offers several years of global know-how and knowledge from Investing as well as experience from the IT sector, the Textile industry, the Pharmaceuticals' industry, the Gaming industry and the Telecom business.


For 25 years The Carying Place has been helping homeless, working families learn the life skills they need to achieve long-term self-sufficiency.
On Saturday, April 21, the Maggy-winning nonprofit will celebrate that record of service to the community with a gala and fundraiser at Prestonwood Country Club in Cary. Beginning at 6 p.m., the event will include family and volunteer testimonials, prized auction items, raffles, a buffet and an open bar. Tickets are $125 per person and are available at https://one.bidpal.net/tcp2018/welcome.
"We work with the entire family, and the goal is to stop the cycle of homelessness," said Executive Director Leslie Covington.
The Cary-based nonprofit provides transitional housing and a 16-week training program in budgeting, goal-setting, time management and self-sufficiency.
"The goal is that children we house will become adults who avoid homelessness, because of their experience and the skills learned by the entire family while in our program," she said.
In addition to financial support, The Carying Place needs volunteers to work one-on-one with families as support partners. The nonprofit could also use skilled workers to help maintain its houses.
The Carying Place has worked with more than 350 families to achieve financial independence. Eight families are currently living in apartments or townhomes owned or leased by the nonprofit. It receives approximately 300 requests for homelessness-related services a month, and there are more than 360 families awaiting housing assistance.


Smishing is a short term for SMS phishing, a variant of phishing email scams that instead utilizes SMS systems to send out bogus text messages. It is the act of using mobile phone text messages to lure victims into immediate action such as downloading mobile malware, visiting a malicious website or calling a fraudulent phone number. These are often seen to be written using fear or greed based terminology such as "fraudulent account activity detected" or by offering some type of award or discount. Often the messages attempt to alarm the potential victim, claiming that urgent action is needed or serious consequences will result – this can be a tell-tale sign that the message you received is dodgy.Smishing can also be used to infect users' phones and related networks with destructive viruses or eavesdropping software. In the age of the smartphone smishing is becoming prevalent.
Many of us keep our personal information, like banking details, stored in our mobile phones. Scammers can access this information through scams such as smishing. While most people are aware of phishing, people generally seem to be less skeptical receiving a smishing message. Scammers are continually becoming more creative in their efforts, while most people won't fall for a "we need your bank account password" email, smishing seems somewhat less threatening. With a 90% open rate of all text messages, it is no surprise that many smish attempts are successful – criminals tend to go where the opportunities are greatest.
Smishing may lead the user to a fake website which will ask the user to complete a form with personal details, no credible business would ever ask you to do this – this is a massive warning sign to any user!
Banks generally won't use text to contact their customers. If they do send texts find out what number they use to generate them so you will know if they are legitimate. The scammers may use spoofed alias numbers that look like they are from your bank, so you should still be skeptical and not reply directly.
Be wary of unsolicited text messages that claim to come from a reputable organisation. Be suspicious of text messages that encourage you to urgently visit a website or call a number to verify or update your details. Do not reply to text messages that request your personal information such as username or bank account without first independently validating that they are genuine. Never respond to text messages which request your 4 digit PIN or online banking password or any other password. Be cautious about clicking on any links that may be embedded or calling the number in a text message.
What is Trump's Merit-based US Immigration? How it Affects You As an Immigrant?


Synopsis: Nikita has been molded into the perfect operative. Meanwhile Michael searches for a way to get the old Nikita back.
At the end of last season, On Borrowed Time, Nikita was subjected to some form of mind control experiment. Michael suspected Section did something and when he asked her, she replied, "Nothing," then with a sad look, "I don't know. Don't remember. I don't love you anymore." Michael is devastated, after a brief pause he walks out. "I won't let them do this."
Madeline and Operations want to test that Nikita is no longer capable of forming emotional bonds. Nikita and Michael are sparring, she seems more aggressive, after taking Michael down she says, "They're watching us." "I know." Later, Nikita is working out at home when Michael calls her in. At the same time her neighbor, Mick, sneaks in to borrow a cup of sugar. Nikita pins him to the floor, gun in hand, questions where he got the key. After he confesses to having made a copy when she asked him to water her plants she lets him up. Back in Section, Michael approaches Walter and asks him to talk to Nikita – see if she's different. Briefing, Operations states that a remote team in Latvia was wiped out except for Irina Kerensky, a level four operative. She must be retrieved or eliminated to protect the information she knows.
A gold taxi pulls up in front of an embassy building, Nikita secretly drops through the floor of the cab and enters a manhole, while the driver converses with a guard. Nikita runs through a corridor and enters the embassy through a basement vent, Michael is monitoring the mission from a van. Nikita cuts through the vent grate, changes into a military uniform, then efficiently takes out three hostiles before proceeding into the next room. Nikita finds Kerensky, the captured operative, behind bullet proof glass – and reports such to Section. Operations asks for her recommendation. Without barely a moments hesitation Nikita replies, "incinerate." Madeline and Operations discuss the consequences of this action and question if Nikita will do it. They tell Nikita to proceed. Nikita arms an explosive, 30 seconds. Michael tells her there are children on the premises. "I hear you Michael, 25 seconds." Michael activates the fire alarm causing the children and most of the occupants to evacuate before the explosion. Nikita calmly walks away from the embassy.
Back at Section, Michael tries to find info about Nikita on the computer, but nothing is available. He meets Nikita on her way home, "I want some answers." He tells her that 16 people died in the embassy. She replies, "I had no choice, they're watching me. I knew you'd activate the alarm." She continues", they tried to reprogram me. It started on the Genefex mission – tried to strip me of my humanity." "Why didn't you let me help you?" "Too much surveillance." Michael recorded the encounter and asks Birkhoff to analyze the eye movement. Birkhoff is reluctant but agrees since its for Nikita. Operations gets off the phone with George, "I could do so much more with George out of the way." Madeline returns, "We tried, for now we have to comply." Nikita enters and they commend her performance on the mission, then Madeline speaks with her about acting with more emotion – starting with Walter. Nikita stops and talks to Walter briefly, but she seems unfamiliar with him. He accepts her act and reports to Michael that she's the same old Nikita. Birkhoff passes his analysis to Michael, Nikita appears to have been subjected to Neural Engineering. A project headed by Dr. Gelman…look for someone close to her, it requires maintenance. Michael rushes to Nikita's apartment, finding Mick there he knocks him down. Then questions him about Nikita. Mick reveals that he's to sprinkle a chemical on the floor and he received the order anonymously. Michael leaves with the vial. Michael meets Birkhoff and finds that four missions to retrieve Gelman failed – he must have taken technology with him. Michael requests a special transmitter from Walter, and gets it. Operations and Madeline speak about Nikita. Operations is pleased with her performance but Madeline reminds him that the first subject was in a controlled environment. This time there are variables we can't control. But Operations remains confident about Nikita.
Mission. The operatives converge on the target and a shoot out erupts. The target flees and Michael pursues him, he knocks the man out and throws him in a closet before reporting to Birkhoff that the target escaped and he's pursuing. Birkhoff gives him one hour, Nikita saw what really happened. Michael meets with Gelman, a sniper is assembling a rifle on a roof top, Michael finds out the mind control is reversible and the process is in Section's lab computer with the original test subject. Michael questions who it was. "If I tell you, you're a dead man." Michael insists and Gelman whispers in his ear. "So that's why you left Section." Sniper takes out Gelman, Michael departs. The team returns to Section and Nikita reports to Operations and Madeline what really happened. And with Madeline's prompting states that the reason Michael is doing it is because he loves her. Operations and Madeline see their opportunity to use Nikita to remove Michael, and get permission from Oversight. Several operatives converge on Michael's office to carry out the cancellation instructions, however they fire into a hologram rather than Michael. "Sir, we have a problem." Michael watches Nikita as she walks down the street.


text:
CN¥
Hotels in California
Hotels with spa in San Diego
This map and the distances to the points of interest are for guidance only. We are not responsible for any possible accommodation location errors.
Based on {{ta_reviews}} reviews
{{/if}} {{#if pvp}}from {{{pvp}}}{{else}}Select{{/if}}
Bahia Resort
998 West Mission Bay Drive,, 92109 San Diego, United States
San Diego, 10.3 km from the city centre. 998 West Mission Bay Drive, see on map
Private Beach Area
The hotel have Outdoor Swimming pool, fitness room, video game room, whirlpool, shuffleboard courts, 2 lighted tennis courts, gift shop,...
See Destinia reviews
Show TripAdvisor reviews
One Market Place, 92101 San Diego, United States
San Diego, 770 m from the city centre. One Market Place see on map
Each guestroom with 340 square ft offers a partial view of the city of Bay, and features one king or two double Grand Beds fitted with...
Best Western Island Palms Hotel & Marina
2051 Shelter Island Drive, 92106 San Diego, United States
San Diego, 6.2 km from the city centre. 2051 Shelter Island Drive see on map
The guest rooms are conveniently located on the ground floor of our two-story buildings and feature one king or two double beds. They...
The Catamaran Resort & Spa
3999 Mission Boulevard, 92109 San Diego, United States
San Diego, 11.8 km from the city centre. 3999 Mission Boulevard see on map
The hotel's 315 rooms and suites are spacious and tastefully appointed with their own balcony or patio. Each has a king or two double...
Hyatt Regency Mission Bay Spa & Marina - San Diego
1441 Quivira Road, 92109 San Diego, United States
San Diego, 9.2 km from the city centre. 1441 Quivira Road see on map
This hotel is conveniently located to all the major attractions and offers views of it's surrounding beauty, making it an ideal option...
Kona Kai Resort & Spa
With a stay at Kona Kai Resort & Spa, A Noble House Resort in San Diego (Beach Cities), you'll be close to Point Loma Nazarene University...
1380 Harbor Island Drive, 92101 San Diego, United States
San Diego, 3.6 km from the city centre. 1380 Harbor Island Drive see on map
All rooms feature the Sweet Sleeper beds and are smoke free. Other amenities include flat screen TV, clock radio, working desk,...
Rancho Bernardo Inn Golf Resort & Spa
17550 Bernardo Oaks Drive, 92128 San Diego, United States
San Diego, 36.4 km from the city centre. 17550 Bernardo Oaks Drive see on map
The guest rooms offer all the comforts you expect; warm rich colors, high speed Internet access, 42 inch plasma screen television, dual...
Hilton San Diego Resort & Spa
1775 East Mission Bay Drive, 92109 San Diego, United States
San Diego, 8.7 km from the city centre. 1775 East Mission Bay Drive see on map
The resort offers comfortable and well-appointed rooms. Each room features a large balcony or patio, king or two queen-sized beds, high...
1404 Vacation Road, 92109 San Diego, United States
San Diego, 9.9 km from the city centre. 1404 Vacation Road see on map
This beautiful private island resort in San Diego, California is full of adventure, excitement, relaxation and fun! Guests can exercise...
Humphrey's Half Moon Inn & Suites
2303 Shelter Island Drive,, 92106 San Diego, United States
San Diego, 5.7 km from the city centre. 2303 Shelter Island Drive, see on map
With a stay at Humphreys Half Moon Inn & Suites in San Diego (Beach Cities), you'll be close to Rock Church and Ocean Beach Park. This...
The Sofia Hotel
150 West Broadway, 92101 San Diego, United States
San Diego, 635 m from the city centre. 150 West Broadway see on map
All rooms offer either one or two beds and amenities include ergonomic work station, air conditioning, flat screen TV, alarm clock...
Hard Rock San Diego
207 5th Avenue, 92101 San Diego, United States
San Diego, 878 m from the city centre. 207 5th Avenue see on map
Studio room has 1 King bed with premium bedding and down comforter. There are 3 restaurants on site. This is a modern style building...
1 Park Boulevard, 92101 San Diego, United States
San Diego, 1.2 km from the city centre. 1 Park Boulevard see on map
A stay at Hilton San Diego Bayfront places you in the heart of San Diego, walking distance from San Diego Convention Center and Petco...
Radisson Suite Hotel Rancho Bernardo
11520 W. Bernardo Court, 92127 San Diego, United States
San Diego, 34.8 km from the city centre. 11520 W. Bernardo Court see on map
With a stay at Radisson Hotel San Diego-Rancho Bernardo in San Diego (Rancho Bernardo), you'll be close to Bernardo Winery and Carmel...
San Diego Marriott Marquis & Marina
333 West Harbor Drive, 92101-7700 San Diego, United States
San Diego, 788 m from the city centre. 333 West Harbor Drive see on map
With a stay at San Diego Marriott Marquis & Marina, you'll be centrally located in San Diego, steps from New Children's Museum and...
The Westgate Hotel
1055 Second Ave, 92101 San Diego, United States
San Diego, 138 m from the city centre. 1055 Second Ave see on map
With a stay at Westgate Hotel, you'll be centrally located in San Diego, steps from San Diego Opera and Westfield Horton Plaza. This...
Hilton San Diego Gaslamp Quarter
401 K Street, 92101 San Diego, United States
San Diego, 758 m from the city centre. 401 K Street see on map
With a stay at Hilton San Diego Gaslamp Quarter, you'll be centrally located in San Diego, steps from San Diego Convention Center and San...
Hotel Iris San Diego
625 Hotel Circle South, 92108 San Diego, United States
San Diego, 5.1 km from the city centre. 625 Hotel Circle South see on map
Location This hotel is located in the famous Mission Valley area, just a short drive from all the major sights. There is a cosy...
Hilton Garden Inn San Diego del Mar
3939 Ocean Bluff Avenue, 92130 San Diego, United States
San Diego, 23.4 km from the city centre. 3939 Ocean Bluff Avenue see on map
When you stay at Hilton Garden Inn San Diego Del Mar in San Diego, you'll be near the beach and close to Torrey Pines Golf Course and...
Find the best hotel and spa in San Diego at an affordable price, thanks to our hotel search engine.
The fast, stressful pace of life means that it is often difficult to take a breather; for this reason people need a relaxing area where they can take things easy, such as a hotel and spa in San Diego. During recent years the demand for hotels with spas in San Diego has grown, as many clients prefer to spend a day relaxing than going out sightseeing. Our hotel and spa filter in San Diego is designed to help you find the one you like most at the best price in the market.
Our database has more than 500,000 hotels which our search engine makes available to you. The best option for finding a hotel and spa in San Diego is our budget hotel search engine, which offers you more than 500,000 establishments, ordered by price.
Hotels by theme
Hotels in the centre of San Diego
Hotels with hot tubs in San Diego
Hotels with pool in San Diego
Luxury hotels in San Diego
Big Bear Lake Palm Springs South Lake Tahoe Anaheim Sacramento Mammoth Lakes San Jose Newport Beach Santa Barbara Long Beach
Salou Benidorm Gandia Torremolinos Playa del Ingles Penyiscola Zahara de los Atunes Cadiz Valencia Mojacar Sanxenxo Lloret de Mar Maspalomas Matalascanyas Malaga Roquetas de Mar Calpe Almunyecar Cambrils Palma Puerto de la Cruz Ibiza Santander Nerja Denia Cadaques Conil de la Frontera Alicante Roses Cullera Chiclana de la Frontera San Sebastian Fuengirola Isla Cristina El Puerto de Santa Maria Las Palmas de Gran Canaria Madrid


This certified organic moisturising fluid moisturises combination to oily skin and gives a lasting matt finish. Flax extract rebalances the skin, purifying it and reducing serum production. Organic hazelnut oil and plant sugars from beetroot moisturise. Organic orange extract boosts the skin's radiance. Natural mineral powders ensure a perfect matt finish. Ultra-light texture.
Tolerance tested under dermatological control. Non comedogenic.
Matte Moisturising Fluid moisturises the skin perfectly. The skin is soft and supple.
The epidermis is purified with a matt finish.
Apply Matte Moisturising Fluid morning and/or night to the cleansed face and neck.
Water, Plant oil**, Organic orange extract*, Emulsifier**, Matifying natural mineral powder (silica), Glycerine**, Organic hazelnut oil*, Beetroot extract**, Gelling agents**, Flax extract**, Thickening agent**, Vitamin E**, Sunflower oil**, Fragrance**, Preservatives.
96.3% of the total plant ingredients are from organic farming.


Below analysis shows how higher passing % in CPT then IPC and Finals is hurting careers of students.
It is harmful for the weaker students as they easily pass CPT but slow down in further levels (IPC and finals) due to increased difficulty level. If level of CPT is improved, students not able to clear CPT exam can pursue other career options rather than investing 6 to 8 years in CA and then not able to find a decent job.
In CPT passing % rate is 30% whereas in Final it is 15% due to which many students who easily enter through CPT are unable to pass out as difficulty level in CPT is very low as compared to IPC and Final exams.
From the above nos. we can easily understand that every six months 35,000 students are passing the CPT exam and only 13,000 students are passing the final exam. Difference of 22,000 students will keep on adding to the student base every six months.
A bit of backward calculation tells us that more than 65,000* students who have entered through CPT are unable to pass out in time.
Great Post. Pictorial comparison in CPT, IPCC and Final is really good.
What is the point of this analysis.. In every course if students are not passing then it is there mistake.
I agree its there mistake, but shouldnt the level of CPT at par with the final exam so student doesnt waste his precious years trying to clear the IPC and Final exams.
Problem is not the increasing no. of students…. rather the quality of students.
IT IS THE MISTAKE OF THE STUDENTS THAT THEY TAKE IT VERY LIGHTLY AFTER PASSING CPT .THEY THINK THAT CPT WAS EASY SO IPC WOULD ALSO BE EASY.


text:
Click the button below to open the link
We use cookies to give you a personalized experience & ads. By using this site, you agree to our cookie policy. Learn more here.
Use Our Safe & Secure Payment Options
Copyright 1997 - 2022, the 888 Group. All rights reserved.
888 Holdings plc is listed on the London Stock Exchange.
Our services are operated by Virtual Global Digital Services Limited, except as stated below. If you have registered from Canada, our services are operated by VDSL (International) Limited. Virtual Global Digital Services Limited and VDSL (International) Limited are incorporated in Gibraltar and are licensed to offer online gaming services by the Government of Gibraltar (Remote Gaming License Numbers 112 and 113), regulated by the Gibraltar Gambling Commissioner under the laws of Gibraltar, and make no representation as to legality of such services in other jurisdictions. Our services in the UK are operated by 888 UK Limited, a company incorporated in Gibraltar. 888 UK Limited is licensed and regulated in Great Britain by the Gambling Commission under the account number 39028.
Our address: 601-701 Europort, Gibraltar.
We encourage responsible Gaming and support GamCare.


Chocolate with hazelnuts. Broken chocolate close up. Antioxidant activity of cocoa.
Tasty chocolate brownies on a wooden table.
Man breaks chocolate bar. Slow motion. Close up. Hands break a bar of chocolate. Closeup.
Hands mixing brown creamy peanut butter in the jar on wooden table top.


text:
Act 1, scene 1 Act 1, scene 2 Act 1, scene 3 Act 1, scene 4 Act 1, scene 5 Act 1, scene 6 Act 1, scene 7 Act 2, scene 1 Act 2, scene 2 Act 2, scene 3 Act 2, scene 4 Act 3, scene 1 Act 3, scene 2 Act 3, scene 3 Act 3, scene 4 Act 3, scene 5 Act 3, scene 6 Act 4, scene 1 Act 4, scene 2 Act 4, scene 3 Act 5, scene 1 Act 5, scene 2 Act 5, scene 3 Act 5, scene 4 Act 5, scene 5 Act 5, scene 6 Act 5, scene 7 Act 5, scene 8 Act 5, scene 9 Act 5, scene 10 Act 5, scene 11
All Themes Ambition Fate Violence Nature and the Unnatural Manhood
All Characters Macbeth Lady Macbeth Banquo Macduff King Duncan Malcolm Weird Sisters
All Symbols Visions and Hallucinations Blood Sleep
Instant downloads of all 1542 LitChart PDFs (including Macbeth).
Teachers and parents! Struggling with distance learning? Our Teacher Edition on Macbeth can help.
Act 5, scene 10
Nature and the Unnatural
Banquo
King Duncan
Weird Sisters
Visions and Hallucinations
Visions and Hallucinations Symbol Analysis
New! Understand every line of Macbeth.
Read our modern English translation.
A number of times in Macbeth, Macbeth sees or hears strange things: the floating dagger, the voice that says he's murdering sleep, and Banquo's ghost. As Macbeth himself wonders about the dagger, are these sights and sounds supernatural visions or figments of his guilty imagination? The play contains no definitive answer, which is itself a kind of answer: they're both. Macbeth is a man at war with himself, his innate honor battling his ambition. Just as nature goes haywire when the normal natural order is ruptured, Macbeth's own mind does the same when it is forced to fight against itself.
Visions and Hallucinations Quotes in Macbeth
The Macbeth quotes below all refer to the symbol of Visions and Hallucinations. For each quote, you can also see the other characters and themes related to it (each theme is indicated by its own dot and icon, like this one:
). Note: all page numbers and citation info for the quotes below refer to the Simon & Schuster edition of Macbeth published in 2003.
Act 1, scene 1 Quotes
Fair is foul, and foul is fair;
Hover through the fog and filthy air.
Related Characters: Weird Sisters (speaker)
Related Symbols: Visions and Hallucinations
Page Number and Citation: 1.1.12-13
Is this a dagger which I see before me,
The handle toward my hand? Come, let me clutch thee;
I have thee not, and yet I see thee still.
Art thou not, fatal vision, sensible
To feeling as to sight? or art thou but
A dagger of the mind, a false creation,
Proceeding from the heat-oppressed brain?
I see thee yet, in form as palpable
As this which now I draw.
Related Characters: Macbeth (speaker)
Get the entire Macbeth LitChart as a printable PDF.
Visions and Hallucinations Symbol Timeline in Macbeth
The timeline below shows where the symbol Visions and Hallucinations appears in Macbeth. The colored dots and icons indicate which themes are associated with that appearance.
Alone, Macbeth sees a bloody dagger floating in the air. He can't grasp it, and can't decide whether it's a phantom... (full context)
...seat at the table. When Lennox gestures at a seat, saying it's empty, Macbeth sees Banquo's ghost sitting there. Macbeth alone can see the ghost. He astonishes the thanes by shouting at... (full context)
The ghost reappears and Macbeth, terrified, starts shouting at it. Lady Macbeth tries to play down her husband's... (full context)
Macbeth in Plain English
"Every teacher of literature should use these translations. They completely demystify Shakespeare. Students love them!"


Please note that the information for Embassy of Vietnam in Philippines may not be up-to-date. If you find any error or have any additional information, kindly contact us to update.
1. Apply visa at Vietnam Embassies in Philippines: Please contact in advance for procedures and fees.
2. Apply a visa on arrival to Vietnam from Philippines with us.


text:
Another Interview From Scratching The Surface, The Porn Documentary
By Recuore, May 17, 2010
Another interview is available for viewing from the work of Francois Driessen in Scratching the Surface, his documentary about the effects of pornography. From the looks of it, the final work is going to be quite good.
The most recent interview is with Crystal Renaud, founder of Dirty Girls Ministries. Read about it, and view it, here.
Crystal explains that the statistics indicate that one out of six women struggle with a strong attachment to pornography, with many seriously addicted, as she was for eight years.
Her interview is very enlightening. She hits on the issues driving involvement from a feminine perspective. She discovered porn at 10 years old and it awakened something in her. She became deeply engrossed in it because she wanted to be the object of affection. She wanted to be cherished and adorned, and her father was emotionally absent, as well as physically absent much of the time.
The desire to be desired is a classic feminine want. A woman wants to be delighted in. And when that desire goes unmet in is proper and healthy way (ie from a girl's father), or is twisted somehow, a woman will often look to others to meet that need.
This is the entry way for women to become addicted to porn, or to get into acting in porn movies. The strong pull of being desired and cherised — even in a corrupted form — fills a void.
In particular, Crystal said something very interesting beginning at the 15:00 mark of the video. She mentioned the role media is playing in the pull pornography has with women in our current age. It's something i notice as a man, but it was nice to hear a women address it.
Crystal mentions that increasingly women are being portrayed as sexually aggressive characters in television programming and on talk shows. They are encouraged to exercise sexual power. She cited an episode Oprah she once saw in which a woman was a guest on the show talking about "erotica," which was essentially a euphemism for porn. "Erotica" was being promoted on the show as a helpful resource for women, giving porn a stamp of approval.
The point of citing this is not to bash Oprah. It is merely to point out that the message today in our media is that porn is acceptable, even desirable, for women as well as men.
Tagged: Dirty Girls Ministries. Crystal Renaud, Francois Driessen, Oprah, Pornography, pornography addiction, Scratching the Surface Documentary about Pornography, Sexualization of Culture
Spin A/K/A Lying
Pastors And Pornography