The ADP-ribosylating enterotoxins, cholera toxin (CT) and the heat-labile toxin (LT-IIa), have been shown to enhance mucosal and systemic antibody (Ab) responses to coadministered antigens. cells, cervical lymph nodes, and spleen. In contrast, SBRCLT-IIaA2/B acquired small influence on B7-2 or B7-1 appearance on B220+, Compact disc11b+, or Compact disc11c+ cells. Evaluation of the useful costimulatory activity of SBR-CTA2/B-treated B cells uncovered a significant improvement in anti-CD3-activated Compact disc4+ T-cell proliferative replies, which proliferation was significantly decreased by treatment with anti-B7-2 however, not with isotype or anti-B7-1 control Abs. Hence, SBR-CTA2/B and SBRCLT-IIaA2/B display distinctive patterns of antibody replies connected with differential results on B7-2 appearance and following costimulatory results on Compact disc4+ T cells. Cholera toxin (CT) made by as well as the labile poisons (LT) from are structurally related heat-labile enterotoxins (HLE) which XL147 have been utilized as adjuvants to augment both mucosal and systemic immune system replies to coadministered antigens (Ag) (3, 12). These oligomeric poisons contain an A subunit noncovalently combined to five B polypeptides (42). After proteolytic cleavage and reduced amount of an intrachain disulfide connection, the A subunit is normally cleaved right into a dangerous A1 and a linking A2 polypeptide. Preliminary research using HLE as mucosal adjuvants in pet models resulted in the final outcome that their adjuvanticity was because of the dangerous ADP-ribosyltransferase activity of the A1 subunit (31). ADP ribosylation from the Gs subunit of adenylate cyclase leads to abnormally high degrees of intracellular cyclic XL147 AMP (cAMP) (24, 42) and following chloride ion efflux in to the lumen from the gut, which is in charge of the characteristic XL147 watery diarrhea ultimately. Because of the dangerous nature from the holotoxins, many researchers have attempted to dissociate the toxicity from the A1-subunit in the adjuvanticity from the Stomach5 complex and also have attemptedto address the immunostimulatory effects of B subunit receptor-mediated interactions. Earlier studies using commercial CTB preparations contaminated with intact CT made it impossible to distinguish between the adjuvanticity associated with ADP-ribosyltransferase activity and the binding properties of the AB5 complex. This issue was further complicated by the synergistic effect of holotoxin on the adjuvanticity of the B subunit (45, 48). However, with the aid of recombinant techniques, mutant constructs of CT and LT-I, which lacked ADP-ribosyltransferase activity, were shown to retain many of the adjuvant properties of the native toxin (11, 15, 52, 53). Studies comparing recombinantly produced wild type and a LT-I B subunit (LT-I B) mutant that lacks GM1 binding further demonstrated that both immunogenicity and adjuvanticity were dependent upon GM1 binding (35). Additional experiments XL147 have demonstrated that the up-regulation of various costimulatory molecules on Ag-presenting cells (APCs) by LT-I B or nontoxic derivatives of CT was abrogated when GM1 binding was blocked (34, 52). These studies demonstrate that the GM1 binding properties of the type I HLE appear to be XL147 necessary for their adjuvant properties. Two types of HLE have been distinguished on the Cd86 basis of distinct immunoreactivity (22, 37): type I HLE are represented by CT and LT-I (25, 37); type II HLE include LT-IIa and LT-IIb (16C18, 23). Although type I and type II HLE are structurally homologous and catalyze similar enzymatic reactions, comparison of the predicted amino acid sequences reveals considerable variability between type I and type II enterotoxin B subunits (22, 37C39). This extensive diversity imparts different ganglioside-binding properties to the respective B subunits. The cellular receptor for CT has been shown to be the monosialoganglioside GM1 (14). The B subunit of LT-IIa binds with high affinity to GD1b and less strongly to GT1b, GD2, GD1a, GM1, and GM2 (14). Gangliosides are glycosphingolipids in which the polar head groups consist of carbohydrate moieties with a lipophilic ceramide tail anchored in the lipid bilayer of membranes (33). Gangliosides are primarily components of the exoplasmic leaflet and have been shown to vary widely at the cell, tissue, and organ level, as well as between species (33). There is significant information demonstrating that various gangliosides play important roles in signal transduction pathways involving cellular immunomodulation, proliferation, differentiation, transformation, and suppression (20, 34, 35, 47, 49). Our laboratory has recently demonstrated that compared to CT, the sort II HLE exhibit specific and powerful adjuvant properties for revitalizing mucosal and systemic immune system.
Advanced hepatic fibrosis therapy using drug-delivering nanoparticles is normally a unexplored area relatively. The cytocompatibility and cell uptake profile of losartan-HA micelles had been examined in murine fibroblast cells (NIH3T3) individual hepatic stellate cells (hHSC) and FL83B cells (hepatocyte cell series). The power of the nanoparticles to attenuate HSC activation was examined in turned on HSC cells Mouse monoclonal to CD22.K22 reacts with CD22, a 140 kDa B-cell specific molecule, expressed in the cytoplasm of all B lymphocytes and on the cell surface of only mature B cells. CD22 antigen is present in the most B-cell leukemias and lymphomas but not T-cell leukemias. In contrast with CD10, CD19 and CD20 antigen, CD22 antigen is still present on lymphoplasmacytoid cells but is dininished on the fully mature plasma cells. CD22 is an adhesion molecule and plays a role in B cell activation as a signaling molecule. predicated on alpha even muscles actin (α-sma) appearance. Mice treated with dental losartan or losartan-HA micelles had been examined for serum enzyme amounts (ALT/AST CK and LDH) and collagen deposition (hydroxyproline amounts) in the liver organ. The deposition of HA micelles was seen in fibrotic livers which implies elevated delivery of losartan in comparison to regular livers and particular uptake by HSC. Energetic reduced amount of α-sma was seen in hHSC as well as the liver organ parts of losartan-HA micelle-treated mice. The serum enzyme amounts and collagen deposition of losartan-HA micelle-treated mice was decreased significantly set alongside the dental losartan group. Losartan-HA micelles showed significant attenuation of hepatic fibrosis via an HSC-targeting system in our in vitro and in vivo studies. These nanoparticles can be considered as an alternative therapy for liver fibrosis. Intro Hepatic fibrosis is an illness affecting a large number of people and it asymptomatically prospects to cirrhosis primarily due to chronic hepatitis computer virus infection alcohol misuse and non-alcoholic fatty liver diseases and causes 30 0 deaths in USA only . Further injury to the liver prospects to cirrhosis in which the architecture of the practical units of the liver becomes disrupted leading to complications such as portal hypertension and even hepatic cellular carcinoma (HCC). HCC associated with cirrhosis is now considered to be among the top ten causes of death worldwide [2 3 The renin-angiotensin system (RAS) is definitely well-known to play an BMS-790052 important part in hepatic fibrosis. RAS parts are overexpressed in hepatic fibrosis one of which is definitely angiotensin BMS-790052 II overexpression that give rise to fibrogenic and inflammatory effects in triggered hepatic stellate cells (HSC) and through angiotensin type 1 (AT1) receptors . In the normal human liver HSC do not communicate AT1 receptors nor do they secrete angiotensin II. Therapies which involve the strategy of focusing on the RAS can be modelled for hepatic fibrosis treatment [5 6 Therefore the AT1 receptors blockade can reduce the activated HSC build up and attenuates liver fibrosis in rats . Though numerous therapeutic strategies have been applied to the reversal of hepatic fibrosis no drug that fulfills this purpose offers yet been successfully launched [8 9 Losartan is an angiotensin II receptor blocker that functions upon AT1 receptors  and is coupled to HSC-specific service providers [11 12 Losartan has been found to inhibit the progression of hepatic fibrosis . The compound is definitely a major candidate in clinical studies as an antihepatic fibrosis drug . The present form of losartan is definitely lipophilic and therefore cannot be given intravenously for improved bioavailability . Losartan was conjugated to mannose 6-phosphate-modified human being serum albumin (M6PHSA) via a platinum linker which was given BMS-790052 to a CCl4-treated rat model for hepatic fibrosis. Losartan-M6PHSA reduced advanced hepatic fibrosis in a short term study . Linker-conjugated losartan therapeutics can deliver only a small amount of drug to the prospective site in comparison to micellar systems. Hyaluronic acidity (HA) is normally a glycosaminoglycan that's abundantly within pet extracellular matrix connective tissues and organs . HA is a biocompatible biodegradable nonimmunogenic and noninflammatory linear and nontoxic polysaccharide . Compact disc44 expression can be increased in situations of hepatic fibrosis [17 18 Compact disc44 have a significant role in turned on HSCs migration during liver organ injury . Compact disc44 is normally a suitable focus on for HA receptor-mediated medication delivery systems . Because of this particular purpose we've chosen a HA BMS-790052 polymer backbone to build up micelles and deliver losartan with a Compact disc44 receptor-based concentrating on mechanism. A prior research reported the feasibility of HA derivatives as book medication delivery providers for the treating various chronic liver organ illnesses including hepatitis and liver organ cirrhosis . Right here the main goal of the analysis was to judge the result of losartan-loaded HA micelles being a targeted therapy for hepatic fibrosis within a mouse model. To verify the result.
Style of site-selective artificial ribonucleases (aRNases) is among the most challenging jobs in RNA targeting. site because of a stacking interaction using the neighbouring nucleotide residue presumably. Altogether the acquired results display that dynamics elements play a significant part in site-specific RNA cleavage. Incredibly high cleavage activity was shown from the GS-9137 conjugates with flexible and prolonged cleaving build which presumably offers a better chance for imidazole residues to become correctly situated in the vicinity of scissile phosphodiester relationship. 1 Introduction The thought of site-selective artificial ribonucleases which can handle cleaving any particular RNA series and conjugatehybrid and determine possible area(s) of cleaving group(s) in accordance with the scissile 5′C63-A64 site. We also display here how the cleaving activity of the conjugates can be governed from the intrinsic GS-9137 properties from the catalytic constructs however not the complete structure from the DNA:RNA cross that was the same in every tests. 2 Components and Strategies 2.1 General Chemical substances All buffers found in the tests were ready using milliQ water contained 0.1?mM EDTA and were filtered through filters with pore size 0.22?(a) Anchor groups used for attachment of dendrimeric RNA-cleaving constructs to oligonucleotide B 5′-phosphate. … 2.3 Cleavage of tRNAPhe??with Oligonucleotide Conjugates [3′-32P]-tRNAPhe was prepared and purified as described in . 3′-end labeled tRNA was dissolved in water and stored at ?20°C. Specific radioactivity of the [32P]-tRNAPhe was 5 × 105?cpm/pmole. Standard reaction mixture (10?as carrier) 5 × 10?7?M [3′-32P]-tRNAPhe and one of oligonucleotide conjugates B-Im(N/m) at concentrations ranging from 5 × 10?7 to 5 × 10?4?M (as indicated in the legends in the figures). Reactions were carried out at 37°C and were quenched by precipitation of tRNA and tRNA fragments with 150?complex. tRNAPhe cleavage by conjugate B-Im(4/1) was performed in the presence of oligonucleotide inhibitors of two types: parent oligonucleotide B and oligonucleotide A complementary to the sequence 61-75 (Figure 2(b) lanes 17-19). As expected the cleavage of tRNAPhe by conjugate B-Im(4/1) is considerably decreased in the presence of oligonucleotides B (primary data not shown) and is completely abolished in the presence of oligonucleotide A which protects target sequence by duplex formation. It is worth noting that in the presence of oligonucleotides B the cleavage of tRNAPhe is suppressed in all sites due to competitive binding whereas oligonucleotides A inhibit tRNAPhe cleavage only at the target sequence failing to change reaction rate at other sites. These Rabbit polyclonal to SCFD1. results indicate the structural specificity of cleavage and stress the fact how the cleavage occurs just at a single-stranded focus on series. Figure 2(c) displays concentration dependencies from the cleavage response for conjugates B-Im(4/1) (3) and Im(24/4+2) (11) (curves 1 and 2 resp.) and binding from the conjugate B-Im(4/2a) and mother or father oligonucleotide B with tRNA (curves 3 and 4 resp.). It really is seen how the cleavage data are in contract with the complicated development. Furthermore the binding affinity from the mother or father oligonucleotide as well as the conjugate researched under identical circumstances are found to become similar (Shape 2(c)). Which means conjugation of cumbersome imidazole-containing reactive organizations towards the GS-9137 oligonucleotide GS-9137 will not influence the hybridization procedure. To estimation the impact of buffer on effectiveness of site-selective RNA cleavage we likened tRNAPhe cleavage from the conjugate B-Im(4/2) in various buffer solutions (Shape 2(d)) used 50?mM imidazole buffer pH 7.0 while a standard circumstances. The pace of site-selective cleavage can be affected by replacement unit of 50?mM imidazole buffer pH 7.0 by 50?mM GS-9137 Tris-HCl buffer or 50?mM cacodylate buffer at pH 7.0 from the factor of just one 1.5 indicating that imidazole itself may donate to cleavage reaction. At much longer incubation period this difference became does and insignificant not really exceed estimated experimental mistake. 50 HEPES buffer entirely abolishes cleavage inactivating the conjugates Surprisingly. The reason of the isn't understood entirely. One explanation GS-9137 could possibly be that sulfonic acidity of HEPES [4-(2-hydroxyethyl)-piperazine-1-ethanesulfonic acidity] may connect to protonated imidazole residues from the conjugates and inactivate it. Previously we observed also.
Oxidative Phosphorylation	GS-9137, Rabbit polyclonal to SCFD1.
Delineating the mechanism(s) that control the specification of hemogenic endothelial cells from primordial endothelium is crucial for optimizing their derivation from human stem cells for clinical therapies. Hence RA regulation of hemogenic endothelial cell specification requires c-Kit notch p27-mediated and signaling cell routine control. Launch Multi-lineage hematopoietic progenitor cells are initial produced from hemogenic endothelial cells from the murine yolk sac Anamorelin HCl at embryonic time (E) 8.25 when the primitive vascular plexus has been remodeled right into a circulatory network (Goldie et al. 2008 Nadin et al. 2003 The standards of arterial venous and hemogenic endothelial cells from primordial endothelium hence occurs concurrently Anamorelin HCl coincident using the starting point of cardiac contraction and pulsatile stream (Lucitti et al. 2007 Delineating the molecular indicators that govern field of expertise of endothelial cell subtypes isn't only vital that you furthering our knowledge of regular vascular advancement but also vital to enhancing methodologies for the aimed differentiation of vascular cells from individual pluripotent stem cells for tissues anatomist and regenerative medication applications. Although we are actually starting to define the signaling pathways that regulate arterial-venous and lymphatic endothelial specification (examined in Atkins et al. (2011)) we still know relatively little about the specification of hemogenic endothelial cells. In earlier studies we defined the phenotype of yolk sac hemogenic endothelial cells (Goldie et al. 2008 Nadin et al. 2003 they communicate the vascular endothelial growth element receptor VEGFR2 (Flk-1) hematopoietic stem cell marker c-Kit and lack manifestation of blood lineage markers including CD45. In addition hemogenic endothelial cells show a Hoechst dye-efflux or SP phenotype which is definitely characteristic of adult hematopoietic stem cells (HSC) and additional stem cell populations (Goodell et al. 1996 Hierlihy et al. 2002 Kubota et al. 2003 Welm et al. 2003 Wulf et al. 2003 Hemogenic SLCO5A1 endothelial cells within the murine yolk sac which demonstrate clonal multilineage hematopoietic potential are therefore defined as Flk-1+ c-Kit+ CD45? SP cells. Our earlier studies also exposed that retinoic acid (RA) signaling is required for hemogenic specification (Goldie et al. 2008 as well as cell cycle control (Bohnsack et al. 2004 Lai et al. 2003 of primordial endothelium mutants is definitely endothelial cell hyper-proliferation associated with decreased manifestation of the cyclin-dependent kinase inhibitors ((mutants is definitely and (Goldie et al. 2008 Importantly we found that provision of bioactive RA to embryos either via maternal feeding (Goldie et al. 2008 Lai et al. 2003 or via whole embryo tradition (Bohnsack et al. 2004 Lai et al. 2003 rescues their flaws in endothelial cell restores and proliferation hemogenic endothelial cell advancement and subsequent definitive hematopoiesis. Hence this model has an ideal hereditary background where to dissect the signaling hierarchy downstream of RA that promotes the blood-forming potential in primordial endothelium and have whether correct endothelial cell routine control is essential and enough for hemogenic standards. Anamorelin HCl We demonstrated that's expressed in the E8 previously.5 murine yolk sac visceral endoderm (VE) while RA receptors (RARα1 and 2) are specifically portrayed by endothelial cells inside the underlying mesoderm (Bohnsack et al. 2004 Goldie et al. 2008 In today's study we utilized mice where the β-galactosidase lacZ reporter is normally expressed downstream of the RA-response component (Rossant et al. 1991 to show that RA signaling is fixed to endothelial cells inside the E8 largely.5 yolk Anamorelin HCl sac as forecasted by receptor expression (Bohnsack et al. 2004 Furthermore 90 of RA-responsive endothelial cells exhibited a hemogenic endothelial cell phenotype had been enriched for multi-lineage hematopoietic potential and portrayed high degrees of and appearance had been also upregulated downstream of Anamorelin HCl was suppressed when Notch signaling was inactivated in (to wildtype amounts) in RA-deficient and Notch-inactivated primordial endothelial cells was enough to improve cell cycle flaws and hemogenic standards therein. Hence our Anamorelin HCl data suggest that c-Kit and Notch signaling function downstream of RA via p27 to modify endothelial cell routine progression which is essential and enough for hemogenic standards. Outcomes Hemogenic endothelial cells.


She isn't the quintessential pen-toting, old-fashioned poet. If you meet her, it's quite likely that you'll see her furiously jabbing away at her smartphone, jotting down poetry on the Google Keep app or unleashing another post into the big bad digital web-world. A self-confessed social media addict, this poetess is quick to shoot down the negativity that surrounds discourses around our digital age by arguing that social media is the most important tool of our age to wage war against rampant apathy. At 21 years of age, she has an extensive following online, is a three-time TEDx speaker on the subject of creativity, and is out with her first book – an anthology of poems. Meet Harnidh Kaur.
Harnidh's poem 'Pantheon' recently garnered her much international attention (not without a fair bit of controversy) for being the inspiration behind a radical set of illustrations 'reimagining goddesses as modern-day feminists', by teen artist Priyanka Paul. The poem struck a chord with several girls who saw themselves in these humanized goddesses, embracing the totality of their imperfections – just as all of us who "fall off pedestals in our daily lives".
For sure, Harnidh's poetry is not easy. It prods you to pause, read again, and wonder. A student of Public Policy at St Xavier's College in Mumbai, her poetry ranges from political to emotional. One of my personal favourites outlines thoughtfully the guilt that plagues an Indian poet who writes in a foreign language.
Tuning into the shared sentiments of thousands of people seems to be the uncanny ability of poets. It certainly explains the loyal following on Harnidh's blog. But, I wonder, how does one bring poetry into daily engagement with data and policies – an apparent antithesis? "Poetry allows me to empathise," she answers. And undoubtedly, if policies are seen as being fundamentally concerned with human life and dignity, then poets would make excellent policy-makers.
But "even a positive thing casts a shadow," as William Irwin Thompson said. And there is often a dark side to poetry. Speaking about creativity and healing, Harnidh emphasises that poetry can frequently steer towards negativity – becoming punishment – by augmenting anger and suffering.
"Poetry is not just catharsis. Poetry is not just rambling. It's very powerful. If you let it be.
This is honest advice, worth sharing with budding poets. But it is, in no way, meant to dissuade them from practicing the power of words in shaping reality.
And when this urgency to speak is met by an equally deep-seated and child-like sense of wonder, the poetry, in its innocence, becomes kinder.
Such conviction is plainly evident in Harnidh's personal dedication to her art. 'The Inability of Words', her first book, is every book-collector's dream – each copy is meticulously hand-bound and hand-stamped in Kolkata by a family of weavers, in a process that takes up to five days. Furthermore, Harnidh aspires to sign personally every single copy that goes out to her readers.
And like any artist who is eager to evolve, Harnidh is on the move already, working on her next project. This new project aims to challenge the oft-prevalent notion that poetry is about 'pretty things'. It instead calls for a radical reimagining of poetry – by seeking out conflict narratives from across India – striving to push to the limit the power of poetry to be able to "take something dark and angry and create something poignant and artful out of it".
The war against apathy rages on. And its warriors continue to show up with words as their sword and armour, kindness as their shield, and a formidable platoon of digital apps.


Immediately after spending some time in Berlin, it is easy to appreciate the people and the differences in their everyday.
The people of the city are liberated and everyone is doing their own thing. Locals don't seem under the big brother spell, opposed to people in say New York, Toronto or Sydney. Berlin has a vibe, and they seem very free-thinking (and free-acting).
That said, Berlin isn't without its quirks. In fact, because you expect Europe to be more "normal" than say other destinations, you tend to get a kick out of each and every peculiarity.
Some things still blow my mind; they make no sense at all. But here, its just the norm.
Who would have thought that there are so many fascinating cultural peculiarities of Berlin?
The traffic lights are green, the pedestrian lights are red, but there isn't a car in sight. Not here, not there, not anywhere.
Nonchalantly, I go to take a step. But out of the corner of my eye, I notice the other 20 people don't even dare.
The pedestrian light is red, and red means stop. So, no one crosses. We all just wait.
I thought it was just me. Maybe I'm too impatient. Maybe all of my travels have rubbed off on me.
But over and over I noticed people would just wait on the quietest streets without a hint of even wanting to cross to get on with the day.
It started to drive me nuts. Just cross goddammit!
I now just cross when the time seems right, but my goodness, the stares!
I read Germans consider it bad etiquette to break rules in front of children. I guess that's a good point if you're grooming them for the Stasi, but then who am I to tell people how to raise their children.
Of course, this isn't a big deal by any stretch, and if anything, I guess it's a little charming that people here are so patient.
While obeying red lights has a certain charm, square pillows are a downright abomination.
I'm not talking about those square decorative pillows that adorn king-sized beds inexpensive hotels.
Nope, I'm talking about regular pillows that you sleep with. And the square 'regular' pillows in Berlin aren't just like normal pillows with four equal sides.
Nope, they're just the same as those huge hotel pillows, but with a consistency more like a bean bag than a pillow.
Now before you judge me as pedantic, just try to sleep with one of these huge beasts.
You know, maybe it's because everyone here is so tall. Maybe those huge square pillows are ergonomically more suited to people over two meters tall.
Nope, I'm not buying it. Berliners, please, abandon the square pillow.
Imagine a shower that's really a bath, except it has a big hose and a shower head that extends from the bath spout.
Oh, except there's no show curtain and there's no fixture to hold the hose above your head. Well, in Berlin, that constitutes a shower.
And it's reached the point where even new houses are implementing this old-world Berliner 'ingenuity'.
Check out Airbnb, Wimdu or any apartments in Berlin and you'll see what I mean. But you won't grasp the total and utter inconvenience until you actually shower in one of these things. You only have one hand free, because the other is holding the unruly serpent, and your entire bathroom becomes soaked.
Berliner shower baths are a good example of needing to leave the past behind.
Where do you live? Oh, number 13? Is it a house? No, an apartment. So, which apartment? Ummm, my apartment. Yeah, but which one is yours?
The one with my name on it. Why is your name on your apartment? How would you know it's mine without my name? Well, you'd have a number.
Why would I have a number, you already know my name. Um… good point.
So yeah, Berlin apartments don't have numbers, they have the names of the occupants.
And those names are written and placed alongside the doorbell. Sounds crazy, but as per my dialogue above, it makes sense.
Criticising the absence of door numbers makes me feel a bit like that American guy off the Amazing Race who said: "[Chinese people] don't even understand their own language". Here I am saying that Berliners need numbers on their doors when all along it just makes sense to have your name.


Mitochondria contain their own DNA (mitochondrial DNA), which is separate from the DNA contained in the cell nucleus. Unlike nuclear DNA, which is in herited from both parents, mitochondrial DNA (mtDNA) is inherited exclusively from the mother's ovum. The mitochondrial genome (16,569 bp) is tiny compared with the nuclear genome (3 X 109 bp). The mitochondrial genome encodes 24 ri-bosomal RNAs, 22 transfer RNAs, and 13 genes coding for subunits of the respiratory chain. The remaining mitochondrial proteins are encoded by nuclear genes. These characteristics result in some unusual genetic and clinical features.
Because there are hundreds of mitochondria per cell, when a mutation arises in mtDNA, that mitochondrion and its descendants contain the mutation, but they coexist with other, normal mitochondria—a situation termed heteroplasmy. When the cell divides, the mitochondria are partitioned between the daughter cells, which may receive varying percentages of normal and mutant mitochondria.
Different organs in the human body have different requirements for mitochondrial energy metabolism. It is generally agreed that the brain, heart, and skeletal muscle have the highest energy demands followed by the kidneys, endocrine system, and liver (34). The phenotypic expression of a pathological mtDNA mutation will be determined by the relative proportion of wild-type versus mutant mitochondria in a given tissue. The percentage of mutant mtDNAs needed to impair energy metabolism enough to cause organ dysfunction is called the threshold effect (35).
The mutation rate in mitochondrial DNA is significantly higher than that observed in nuclear genes. The accumulation of sequence polymorphisms has been estimated to occur 17 times faster in mtDNA than in nuclear DNA (36,37). This has been attributed to the generation of free radicals from oxidative phosphorylation (which causes DNA damage) as well as to the paucity of DNA repair mechanisms in mitochondria as compared with the nucleus (38).


text:
Q: What does "degrees of freedom" mean in the context of cointegration? I recently read a paper where the author says they used cointegration "with two degrees of freedom". What does degrees of freedom mean in this context?

A: I'm speculating here without more context, but if two or more nonstationary processes are cointegrated, there is some linear combination of the processes that is stationary.  Said another way, the processes revert to some mean state with respect to one another.
These stochastic processes could be multidimensional (e.g. $Y_i$ moves with respect to two dimensions) and has more than one 'degree of freedom,' so to speak.
I concede that this is very confusing because of the meaning that the phrase 'degrees of freedom' have in the stats context.

redpajama_set_name: RedPajamaStackExchange


text:
Click here to exit site quickly!
Need Help Now? • 24-hour number 306-525-2141 or 911 • Mobile Crisis Helpline 306-757-0127
Family Homelessness
Program Documents
YWCA Camps
Women of Distinction Awards
Walk A Mile In Their Shoes
Management Directory
Local Women's March to Focus on MMIWG this January
Regina – YWCA Regina is hosting the city's second annual Women's March on Saturday, January 19, 2019. The event invites community members of all ages, genders, races, abilities, and religions to join the global #WomensWave in the spirit of Truth and Reconciliation by wearing the colour red. The March will begin at 10AM at the YWCA, 1940 McIntyre St., with music by special guest Natural Sympathies kicking things off at 9:45AM.
"We've chosen to honour the lives of Missing and Murdered Indigenous Women and Girls as the cornerstone of this March," says YWCA Regina CEO Melissa Coomber-Bendtsen. "We recognize how important it is for us to stand up for each other and march not just for ourselves, but to empower one another."
The event seeks to inspire, unite, and lead the charge for the advancement of women across Canada, while shining a light on gender inequality in Regina and the surrounding area.
"The Women's March offers residents an opportunity to imagine what a community rooted in solidarity and pursuit of gender and social justice looks like," continues Coomber-Bendtsen. "By choosing to shine a light on Missing and Murdered Women and Girls in our province and amplifying the cause with our voices, we are grounding ourselves in that knowledge and committing ourselves to following the leadership of Indigenous communities to bring about an end to the crisis."
Women's March Canada is a Canadian national movement to unify and empower everyone who stands for human rights, civil rights, and social justice for all. To learn more, visit www.womensmarchcanada.com/regina.
https://ywcaregina.com/wp-content/uploads/2020/04/womens-march.jpg 1080 1920 Carter https://ywcaregina.com/wp-content/uploads/2019/12/YWCA_Regina_Logo_Black.png Carter2020-12-20 09:59:172020-04-02 12:00:54Local Women's March to Focus on MMIWG this January
Happy Holidays from YWCA ReginaDecember 20, 2022 - 12:51 pm
We Remember: December 6, National Day of Remembrance and Action on Violence Against WomenDecember 6, 2022 - 12:50 pm
Walk A Mile in Their Shoes 2022 Walker BibsAugust 10, 2022 - 10:06 am
YWCA Regina AGM NOTICEMarch 31, 2022 - 11:05 am
Welcome to Your YW: Winter/Spring 2022 NewsletterJanuary 25, 2022 - 3:35 pm
Centre for Women and Families Community Consultation SessionFebruary 11, 2020 - 10:07 am
YWCA Regina launches Coldest Night of the Year 2020January 20, 2020 - 9:33 am
YWCA Regina
1940 McIntyre Street, Regina SK S4P 2R3
Email: ywcaregina@ywcaregina.com
YWCA Regina Inc. is accredited under the Imagine Canada Standards Program.
The Standards Program Trustmark is a mark of Imagine Canada used under license by YWCA Regina Inc.
About | Programs | News | Events | Contribute
© YWCA Regina 2020 | Made with ♥ by Strategy Lab
YWCA Regina Newsletter – April 2020 YWCA Regina Women's March to Move #ForwardTogether Virtually

redpajama_set_name: RedPajamaCommonCrawl


On an abandoned moon base, the Twelfth Doctor discovers a flesh-eating horror that leaves only the bones of its victims behind. Can the Doctor stop this monstrous being before it's too late?
At first glance, this book is very attractive to the classic gamebook fan. It's chunky, with a brightly-colored spine sporting the Penguin logo, and it definitely evokes the classic Fighting Fantasy look and feel. Unfortunately, though, while the format implies a lengthy gamebook, perhaps featuring some complex mechanics, the reality is that these are simple pick-a-path books, and the thickness is made up mostly with empty space -- the text is double-spaced, and if the blank lines were removed, you would end up with something about the length of the earlier Doctor Who: Decide Your Destiny books.
Issues of packaging wouldn't matter so much if this were a great book, but unfortunately it is not. The blend of sci-fi body horror and Doctor Who tropes on display here never meshes quite right. The graphic content -- flesh dissolving, bodies splitting into horrifying monsters -- seems a bit strong for something published under a "for children" banner, yet some of the more juvenile Doctor Who conventions -- particularly the need to reduce the threat to a ranting monster that could be rendered as a rubber suit -- undermines the horror. I did appreciate the way the author drew on both classic and new series elements to pepper the text with fan-friendly references, but I would have appreciated a better story a lot more than fan service.
The gameplay of the book is also rather lacking. The fact that this is written in the third person and that the Doctor is essentially an invincible character significantly reduces reader involvement in the text. The use of a lot of arbitrary choices, some of which are about "what happens next" rather than "what to do next," doesn't help. I can think of two ways in which this book could have been a lot more interesting. One would have been to create a more compelling cast of secondary characters, and creating tension by making it difficult or impossible for the Doctor to save all of them -- the book at least hints that this idea crossed the author's mind, but the characters are too scattered and underdeveloped to get much of a reaction out of the reader. Another option would have been to create a more interesting monstrous foe, and allow the reader to learn more about it by exploring different paths -- the book certainly shows different aspects of the creature in different paths as written here, but it never gels together into a particularly coherent whole, instead reading like a random collection of potentially gross and/or scary atttributes.
I approached this book with fairly low expectations, and unfortunately they were not exceeded. It's a shame, since the book combines two things I enjoy: claustrophobic monster tales and Doctor Who; however, that's a challenging blend to handle well, and the author missed the mark more often than he hit it in this case.
Thanks to Mateusz Motyka for the cover image.


Barney & Buddy is an independent family run business based in the beautiful city of Bath. The Founder Jeremy Boreham has almost 20 years' experience in the toy and publishing industry (in other words, playing with toys and reading kids' books all day!) and is supported by his wife and two sons (yep, you guessed it, they are called Barney & Buddy – the real brains behind the business!) We are passionate about toys and we aim to create fun yet educational toys for little ones around the world. It helps having two energetic boys under the age of 4 as our chief product testers!
We design and produce high quality children's toys (with a few novelty books-plus items thrown in). Our ethos is all about creating products that are FUN but which encourage active learning through play. We make toys that we love and more importantly, that our boys love. If it doesn't get the nod from our little chief product testers, it doesn't get produced!
Our Aim – We aim to create educational toys and books that are designed to nurture and enhance children's development in their early years. We try to make learning fun and engaging with our innovative range of products.


Data Recovery 2300peterw 2300peterw replied to How can I recover data on non-system RAID drives when OS is fried?
Firewalls 2300peterw 2300peterw replied to When does a firewall have to be replaced?
Android 2300peterw 2300peterw replied to what is future of android system?
ASUS Hardware 2300peterw 2300peterw replied to Can someone make sense of my router logs?


FOB Reference Price:Get Latest Price food, metallurgy, building materiel, machinery, mine,and other industries. This equipment occupies very little room, and the totally sealed structure can User manual in PDF and Printed copy.
1992 products Inspired by your Recent Search: View More . Engineers available to service machinery overseas. 2018 Licheng Machinery manual pdf manual brick making machine 6 YRS Jining Hengwang Mining Machinery Co., Ltd.
45 products Activated Carbon Coconut Shell Gold Ore Dressing Agent for Mining. US $1500 2200 Gold sorption resin D301G used machine &equipment.
1231 products 7 YRS Shijiazhuang An Pump Machinery Co., Ltd. land irrigation, garden spray, well, water tower, urban construction, industries and mines.
Sbm Low Price High Quality Primary Stone Jaw Crusher Pdf , Find Complete Details Home >; All Industries >; Machinery >; Energy &Mineral Equipment >; Mining Machinery >; Crusher (195870) FOB Reference Price:Get Latest Price.
55 products 4 YRS Guilin Hongcheng Mining Equipment Manufacture Co., Ltd. 80.0% Diorite Excavator Jaw Crusher Equipment Pe 400 X 600 Pdf Price List.
55 products A wide variety of ball mill design pdf options are available to you, such as free samples. Mining ball mill metal powder grinding , ball mill design pdf for sale . 10 YRS Henan Zhengzhou Mining Machinery Co., Ltd. Trade Alert Delivering the latest product trends and industry news straight to your inbox.
FOB Reference Price:Get Latest Price . chemical, steel, mining, oil equipment, temperature monitoring, boilers, large air conditioning and other industries.
9356 products Tags: Welding Wire Drawing Machine Lathe Machine Drawing Pdf Pdf Tube Cold Drawing . Newest cold drawing pipe equipment tube drawing machine . 3 YRS Shandong Xin Mei Mining Equipment Group Co., Ltd. 85.2%.
1879 products Add to Compare. Tags: Jaw Crusher Drawing Manufacturer Mining Big Iron Perfect Jaw Crusher Jaw Crusher stone Jaw Crusher Drawing For Mining Equipment to Compare. Tags: Crusher Pdf Grinding Plant Trade Alert Delivering the latest product trends and industry news straight to your inbox.
Machine Xy 1 Xy 1b Vertical Driller/drilling Machine,Drilling Rig Pdf,Drilling Rig Hand,Job Drilling Rig from Mine Drilling Rig Supplier or Manufacturer Hubei Suizhou Lilong Machinery Co., Ltd. FOB Reference Price:Get Latest Price.
46750 products Mobile Jaw Crusher Machinery/Mine Quarry Crusher/Mobile jaw Crusher . stone quarry crusher machine for sale, jaw crusher pdf manufacturer.
Stone Grinding Cutting Jaw Stone Crusher Machine Cost,Jaw Crusher Pdf , Find Home >; All Industries >; Machinery >; Energy &Mineral Equipment >; Mining Machinery >; Crusher (195870) FOB Reference Price:Get Latest Price.
Avoid upfront costs and provisioning of expensive equipment by only paying for the resources used. Operation &. Network in China. Download the PDF.
bitcoin miner usb,bitcoin miner,asic mining circuit usb pcb board. FOB Reference Price:Get Latest Price. US $0.1 1 Pieces 1 Piece/Pieces (Min. Order).


text:
Analyst - Abel Nevarez Analyst - Brian Huh Analyst - Constantinos Papavassilopoulos Analyst - Craig Stice Analyst - Edurne Zoco, PhD Analyst - Kia Ling Teoh Analyst - Michael Howard Analyst - Ryan Darrand Geography - South Korea
Chenyu Cui (40)
Kia Ling Teoh (7)
Richard Son (1)
Xin Zhang (8)
Africa and Middle East (37)
Rest of Asia Pacific (13)
South East Asia (11)
Advertising Intelligence Service (2)
Home Entertainment KPIs Intelligence Service (1)
TV & Online Video Intelligence Service - Premium (2)
Korean broadcasters join forces with SK Telecom to step up original content production
Kia Ling Teoh | Jun Wen Woo | January 28, 2019
Korean telco SK Telecom has signed a memorandum of understanding with the country's three leading broadcasters to integrate their online video platforms, Oksusu and POOQ. Broadcasters Korean Broadcasting System (KBS), Seoul Broadcasting System (SBS) and Munhwa Broadcasting Corporation (MBC) operate POOQ through a joint venture called Content Alliance Platform. The new service will aim to create more local original content to fend off growing competition. According to reports, SK Telecom CEO Park Jung-Ho was seeking an initial investment of $180 million to produce original content; in comparison, Oksusu spent just $10 million on content last year. The company also aims to expand the new online video platform to Southeast Asia later in the year.
Netflix to add Indian and Korean to its global original kids slate
Kia Ling Teoh | October 20, 2017
Netflix announced its first original children's series productions with Indian and South Korean partners at the MipJunior event in Cannes this week. Andy Yeatman, kids and family director, also gave some insight on the importance of children's content to the global streaming service. The two new productions, Mighty Little Bheem and Yoohoo & Friends, are based on established kids characters in each territory and will be launched in 2018.
South Korean broadcasters launch Kocowa, a new online streaming service in the US
Kia Ling Teoh | Jun Wen Woo | July 28, 2017
Three of South Korea's leading broadcasters have launched Kocowa (Korean Content Wave), an online video streaming service, in the US. The service, launched on 24 July, is backed by Korean Content Platform (KCP), a US-based joint venture company between Korean Broadcasting System (KBS), Munhwa Broadcasting Corporation (MBC) and Seoul Broadcasting System (SBS). The service offers content from these broadcasters with English subtitles, with some shows airing six hours after broadcast in South Korea.
Market Monitor: Advertising in APAC
Kia Ling Teoh | March 15, 2017
The 2016 APAC ad market was bolstered by a robust performance of traditional media and the rapid growth of online. In this Market Monitor, we look at the market performance of top ten APAC ad markets, and the key players that shape this thriving regional ad growth.
World TV Production - 2016
Tim Westcott | Kia Ling Teoh | Matteo Marchello | October 27, 2016
Consumers have embraced internet-connected devices to view more programming online and on-demand. Linear TV companies are ramping up investments in content to stand out in a multi-platform world, while a spate of mergers and acquisitions has created ever-larger content-creating groups.
Video is the future of TV apps
Jack Kent | Abel Nevarez | August 30, 2016
Beyond video apps and services, many of the first wave of TV apps have been built out of existing smartphone apps. The TV apps environment presents developers with a number of new challenges.
Regional and international programmers talk up Asian co-production
Kia Ling Teoh | December 04, 2015
This week's Asia TV Forum and market in Singapore was attended by TV programme creators, distributors and buyers from inside and outside Asia. During the pre-market conference, key messages from both global and regional TV players were: Global production companies are looking for co-production opportunities in Asia rather than just selling finished programmes; Asia's content creators are looking to create more original content that cater not only to the local audience but also the international market; and China's TV broadcasters are ready to go digital and international.
The business of children's content
Tim Westcott | Kia Ling Teoh | October 20, 2015
In a world of declining linear TV viewing and increased use of internet-connected smartphones, tablet computers and other devices, the children's TV genre remains in good health. Programming has largely migrated from generalist TV channels to more than 400 dedicated channels aimed at the under 12 audience worldwide, with a total of $2.165 billion invested on children's programming last year.
Apple Music can kick start on-demand music business
Jack Kent | Abel Nevarez | July 03, 2015
Apple has made its long expected entry into the on-demand subscription music market with the launch of Apple Music: a premium only service with plans starting at $9.99/month for personal plans and $14.99/month for the family plan in the US. Apple Music will be available as a free trial for the first three months. Unlike its App Store policy, Apple Music launches with differential pricing based on geography and is available for much lower prices in less developed markets. In contrast to its App Store policy, Apple Music launches with differential pricing based on geography and is available for much lower prices in less developed markets.
Operators must partner to succeed in value added services
Abel Nevarez | June 25, 2014
Innovative operator strategies are now focused on using content and services to drive data usage, increase ARPU, and attract and retain customers, rather than for direct monetization. The challenge for most mobile operators is not whether to directly monetize such services, but how to use them to support other revenues lines and engage customers.
Messaging service Line eyes global revenue growth with new content services
NHN Corp's Line mobile messaging service will introduce new video, e-commerce, and music services later this year. The new services will follow Line's successful rollout of a games platform that has helped...
Major upgrade to mobile music data
Jack Kent | Abel Nevarez | March 21, 2013
IHS Screen Digest has upgraded its mobile music coverage with enhanced coverage of mobile subscription (on-demand, radio, bookclub), full track retail (album and single) and personalisation services (ringback...


>Unique palette of multi-colored pearls blends together to creaate an instantly healthy-looking glow for cheeks.
>Ultra-blendable powder blush delivers a soft wash of color with a luminous finish.
INGREDIENTS : Mica. Kaolin. Polyethylene. Octyldodecyl Stearoyl Stearate. Pentaerythrityl Tetraisostearate. Nylon-6. Butyrospermum Parkii (Shea) Oil. Lauroyl Lysine. Hydrogenated Coco-Glycerides. Acrylates / C10-30 Alkyl Acrylate Crosspolymer. Pearl Extract. Bis-PEG-12 Dimethicone Beeswax. Caprylyl Glycol. Microcrystalline Wax. Silica. Polybutene. Tocopheryl Acetate. Retinyl Palmitate. Phenethyl Alcohol. May Contain: Iron Oxides. Titanium Dioxide. Carmine. Synthetic Fluorphlogopite. Tin Oxide. Ultramarines. Yellow 5 Lake.
Hypoallergenic. Fragrance free. Paraben free. Non-Comedogenic. Dermatologist approved.
ARDELL Complete Brow Grooming Kit - Beautiful Brows!


text:
PATH does not Consider China a Recipient, but a Partner
The focus of the previous post on this blog is on the role China plays in the strategy of ROI3, Inc. In doing some research for the post, I found some notes that I took while attending an event at The Bureau of Asian Research (NBR) on March 15, 2013 entitled "China's Evolving Health Industry Investments" featuring Jiankang (Jack) Zhang, PATH's China Country Program Leader. While the event occurred eight months ago, there are a few points that remain relevant and worth sharing in this post. [Photo of Jack Zhang: PATH/Mike Wang]
Program for Appropriate Technology in Health (PATH)
"PATH's mission is to improve the health of people around the world by advancing technologies, strengthening systems, and encouraging healthy behaviors," according to its website. The Seattle, Wash.-based international nonprofit organization, which was founded in 1977, says that it "transforms global health through innovation. We take an entrepreneurial approach to developing and delivering high-impact, low-cost solutions, from lifesaving vaccines and devices to collaborative programs with communities. Through our work in more than 70 countries, PATH and our partners empower people to achieve their full potential."
In 2011, according to its Consolidated Financial Statements, PATH's total revenue was $283,838,000. Their funding derives from foundations; the United States government; other governments, other nonprofit organizations, and multilateral agencies such as the World Health Organization (WHO); individuals; and interest from investments 75.5 million people worldwide benefited from PATH's work in 2011 including 4.8 million mothers, newborns, and infants; 8.3 million children and adolescents; and 5.4 million people living with HIV/AIDS or tuberculosis.
Specific to China, PATH, since 1979, "has worked with Chinese government agencies, nongovernmental organizations, research institutes, and manufacturers to improve health. Our team in China has focused particularly on addressing reproductive health and infectious diseases." Coordinated from the organization's office in Beijing since 2003, PATH's strategy for success includes:
Developing and expanding public-private partnership models for health product development that take into account health needs, the best interests of donors, PATH's core competencies, the interests of Chinese public agencies, and synergies with Chinese public and private companies;
Identifying opportunities vertically and horizontally to build on this model to meet the multiple health needs of vulnerable populations in and outside of China; and
Promoting global access and supply of affordable, accessible, and sustainable health products in China, and through China to other low-resource settings.
PATH in China explains how "PATH views collaboration as the key to providing innovative, sustainable, affordable, and culturally appropriate health solutions for low-resource settings. We conduct rigorous monitoring and evaluation to ensure the efficiency and effectiveness of our work. PATH's strategy in China includes:
Developing and expanding public-private product development partnerships that align with donor interests, PATH's core competencies, the interests of China's public health agencies, and the needs and perspectives of Chinese companies;
Identifying opportunities to build on these partnerships to meet multiple health needs of vulnerable populations inside and outside of China; and
Promoting sustainable access to supplies of affordable health products in China and through China to other low-resource settings.
PATH's specific initiatives in China include work in vaccine research, development, and introduction; Woman's Condom product development and promotion; and new diagnostic tools for tuberculosis (TB). A sampling of PATH's projects includes the advancing rotavirus vaccine development; assessing Chinese vaccine manufacturers, the Japanese Encephalitis project; polio vaccine development and scale-up project (PVD); Protection Options for Women (POW); the tuberculosis new diagnostic demonstration project; the Woman's Condom project; and the Safe Water Project.
"China's Evolving Health Industry Investments"
As PATH's country program leader in China, Jiankang (Jack) Zhang is responsible for program development, project management, office management, and liaising with local and international collaborators, including local health authorities, public and private institutions, and other nongovernmental organizations in China.
During his presentation, Mr. Zhang said that the Chinese government is encouraging more private investments in the pharmaceutical and biotechnology sectors. He noted, however, intellectual property protection remains a significant concern by private enterprises, particularly companies based outside of China.
In Mr. Zhang's interview with NBR's Claire Topal and Karuna Luthra on May 25, 2011 entitled "A Pivotal Moment for China and Vaccine Manufacturing," it is noted, "The World Health Organization (WHO) announced on March 1, 2011, that the national regulatory authority of China—the State Food and Drug Administration (SFDA), along with affiliated institutions—now meets WHO indicators for a functional vaccine regulatory system. This means that Chinese-made vaccines are now eligible to apply for WHO pre-qualification."
WHO prequalification ensures that vaccines used in national immunization services in different countries are safe and effective for target populations at the recommended schedules, and that they meet particular operational specifications.
During the 2011 interview, Mr. Zhang said, "WHO's clearance opens the door for [Chinese vaccine manufacturers] to apply for WHO vaccine pre-qualification—a regulatory status that opens the door for United Nations agencies and governments to begin ordering the vaccine— with the aim of becoming eligible for vaccine procurement by the United Nations Children's Fund (UNICEF)."
Responding to a question, during the 2011 interview, on the implications of the announcement for China's role as an international partner in global health, Mr. Zhang explained, "The Chinese health authority considers the WHO clearance a significant step in China's efforts to contribute to global health, notably to African countries where China has already donated to infrastructure, health systems, and anti-malaria treatment and control programs. The addition of vaccines to this portfolio is truly exciting for the Chinese government."
However, according to Mr. Zhang, "Obtaining a WHO pre-qualification certificate is complex and can be difficult to maintain. Ultimately, in my opinion, the biggest challenge for China won't be in building infrastructure, but instead in sustaining the commitment of public agencies and encouraging manufacturing executives to strictly comply with WHO requirements and the new Good Manufacturing Practice code."
The new Good Manufacturing Practice (GMP) code, adopted by the SFDA and effective March 1, 2011, aligns with WHO GMP standards, containing stricter requirements for the production of pharmaceuticals (including vaccines). The SFDA has asked that all newly-built pharmaceutical manufacturing enterprises should comply with the new GMP code. Existing factories, which produce sterile drugs, including blood products, vaccines, and injections, are mandated to reach the new code before the end of 2013. The deadline for other plants is Dec 31, 2015. Companies that cannot meet the new requirements before these deadlines will be forbidden from continuing to produce drugs. See SDA website, www.sda.gov.cn/WS01/CL0844/59017.html.
While Chinese companies may apply for WHO pre-qualification to manufacture vaccines for the African market, Mr. Zhang remarked during his presentation on March 15th that most vaccines used in Africa are manufactured in India. Despite the lack of Chinese-manufactured vaccines in Africa, he emphasized that the 4th International Roundable on China-Africa Healthcare Cooperation, which is scheduled for May 2013 in Gaborone, Botswana, will attract senior government officials and company executives from China and throughout Africa alike. (The World Bank published an article on December 10, 2009 about the initial China-Africa Healthcare Roundtable that was held in Beijing from December 4-5, 2009. This article by UNAIDS dated May 7, 2013 provides a summary of the aforementioned conference held in Botswana.) (Photo: UNAIDS)
Upon the conclusion of Mr. Zhang's presentation, I asked for an update on how China's vaccine manufacturers were complying with WHO requirements including the GMP. He replied that while Chinese-made vaccines are now eligible to apply for WHO pre-qualification, not a single manufacturer has yet be approved for pre-qualification.
While polio was eradicated in China, Mr. Zhang noted tuberculosis remains a problem. PATH produced a document, Collaborating to Control Tuberculosis, about its work to combat TB in China that says, "China has the most MDR-TB (management of multi-drug resistant TB) cases in the world and the second largest number of overall TB cases. The World Health Organization classifies China as a high-burden country. There are an estimated 120,000 new cases of MDR-TB each year in China, accounting for 24 percent of the global total."
Furthermore, according to the TB document, "With funding from the Bill & Melinda Gates Foundation, PATH is responding to the challenge. In collaboration with the Foundation for Innovative New Diagnostics and the Hong Kong Supranational Reference Laboratory, PATH assists the Chinese National TB Reference Laboratory with determining the operational feasibility, cost-effectiveness, and impact of new TB diagnostics in order to help the Chinese Ministry of Health decide whether to introduce and scale up these technologies."
Regarding his organization's operations in China, Mr. Zhang remarked that "PATH does not consider China a recipient, but a partner."
Aaron Rose is a board member, corporate advisor, and co-founder of great companies. He also serves as the editor of GT Perspectives, an online forum focused on turning perspective into opportunity.
Labels: Asia, Bill & Melinda Gates Foundation, China, global health, mHealth, PATH, sustainable development
Tech Summit Aims to Connect Leaders From China and...
PATH does not Consider China a Recipient, but a Pa...
Understanding China - Its Role in Global Business
How to be a Successful International Entrepreneur


The Napa Valley is comprised of many small communities, each with its own unique identity that invites meandering exploration. Historic Main Streets and downtowns are lined with locally-owned boutique shops, restaurants and tasting rooms each offering unique Napa Valley experiences.
Visit downtown, the city and more!


And the answer is simple: Because you're tired of the "normal" Santa Claus stickers and you want something no-one else has. Something shocking! Something fun! Something outrageous! So to celebrate christmas we have put together the most fantastic sticker pack you'll ever see. More than 100 stickers with Santa and his friends celebrating, partying and simply put GOING CRAZY! Shocking Santa has got something for everyone, download it now and spread the X-mas love to all your family and friends!
He will knock U out!
In more than one way!


text:
Unemployment High Due To Govt's 'Ill Considered Demonetisation', Says Ex PM Manmohan Singh
Shweta SengarUpdated on Mar 03, 2021, 14:55 IST
Hitting out at the Centre over the high unemployment rate in the country and low economic growth, former prime minister Dr Manmohan Singh blamed demonetisation for the crisisAttacking the Centre, Singh said unemployment is high in the country and its informal sector is in shambles following the "ill considered demonetisation decision" taken by the BJP-led government in 2016He also criticised the central government for not holding regular consultations with the states
Hitting out at the Centre over the high unemployment rate in the country and low economic growth, former prime minister Dr Manmohan Singh blamed demonetisation for the crisis.
Attacking the Centre, Singh said unemployment is high in the country and its informal sector is in shambles following the "ill considered demonetisation decision" taken by the BJP-led government in 2016.
He also criticised the central government for not holding regular consultations with the states.
Singh also said temporary measures by the central government and the RBI to paper over the credit problem cannot blind us to the looming credit crisis ahead that could affect the small and medium sector.
"Unemployment is high and the informal sector is in shambles, a crisis precipitated by the ill considered demonetisation decision taken in 2016", he said at "Pratheeksha 2030."
The summit was organised to launch a Vision Document, a framework of ideas for development of Kerala well in advance of the state elections.
Unemployment is high & the informal sector is in shambles- a crisis precipitated by the ill-considered Demonetisation decision taken in 2016.
- former Prime Minister Dr. Manmohan Singh#modi_jawab_do pic.twitter.com/rvQeWAR9m9
— Congress (@INCIndia) March 3, 2021
Many states in disarray
He observed that in Kerala and many other states, public finances were in disarray with states having to resort to excessive borrowing, which creates an intolerable burden on future budgets.
"Federalism and regular consultation with states, which was the cornerstone of India's economic and political philosophy as enshrined in the Constitution, no longer finds favour with the present Central government," he said.
Singh said while Kerala's social standards are high, there are other sectors that need strong attention in the future.
"There are many roadblocks ahead that the state has to overcome. The global downturn of the last two or three years, aggravated by the pandemic, has made the global interface of Kerala more fragile.


Next K9-85 MCW is a PowerCode™ wireless digital PIR detector designed to enable homeowners to secure their homes while allowing their pets to move freely anywhere in the house. Employing Visonic's patented "Target Specific Imaging™" optical system (TSI) and unique pet identification software algorithm, the Next+ K9-85 MCW has an unprecedented capacity to reliably distinguish between human bodies and pets weighing up to 38 kg (85lb).
The result is the industry's best detection accuracy, false alarm immunity and ultimate long term reliability – in an attractive, easily installed wireless detector designed for any pet-loving home environment.
SecureMAX is one of the few Security companies in London that can supply 24 hour response for all home and business Alarm Systems and CCTV Cameras systems. Our response services are provided and coordinated through our Security Control Centre, located in East London. We can provide a wide coverage within M25 London and Essex with the assistance of our professional network.


G6PD Deficiency is an inherited condition; therefore, you can not get it from being in contact with someone who has G6PD Deficiency. Since it is inherited, there is no cure.... Fava Beans. This is the number one "no-no" food for the child and has been proven to trigger the symptoms. This is so related to the deficiency that G6PD may be sometimes referred to as "favism" in reference to this food.
The carbohydrates that cause the most problems include white flour, refined sugars, and high fructose corn syrup, Additional food products that can cause G6PD deficiency syndromes to appear include tonic water, items that use blue food coloring, and products … canada food guide 2015 pdf How does someone get G6PD deficiency? It is inherited – that is, it is passed on at birth from one or both parents to the child. Most commonly it is passed from the mother.
Foods to Avoid When Suffering from G6PD Deficiency People with deficiency need to avoid certain food items like peanuts, legumes, products that contain menthol, etc., which trigger reactions. Here is a list of the foods that should be refrained from in your diet.


text:
Q: ubuntu 18.04 apt-get upgrade problem "redirection loop encountered." I have recently upgraded from Ubuntu 16.04 to 18.04. Now, when I run apt-get upgrade, I get the following error.
larry@VUbuntu15:~$ sudo apt-get upgrade
[sudo] password for larry:
Reading package lists... Done
Building dependency tree
Reading state information... Done
Calculating upgrade... Done
The following packages have been kept back:
  linux-generic linux-headers-generic linux-image-generic
The following packages will be upgraded:
  libvte-2.91-0 linux-libc-dev
2 upgraded, 0 newly installed, 0 to remove and 3 not upgraded.
Need to get 1,146 kB of archives.
After this operation, 0 B of additional disk space will be used.
Do you want to continue? [Y/n] y
Err:1 http://192.119.223.46:80/data/0116fed259cd0925/us.archive.ubuntu.com/ubuntu bionic-updates/main amd64 libvte-2.91-0 amd64 0.52.2-1ubuntu1~18.04.2
  Redirection loop encountered
Err:2 http://192.119.223.46:80/data/011634d2c0cdf482/us.archive.ubuntu.com/ubuntu bionic-updates/main amd64 linux-libc-dev amd64 4.15.0-30.32
  Redirection loop encountered
E: Failed to fetch http://192.119.223.46:80/data/0116fed259cd0925/us.archive.ubuntu.com/ubuntu/pool/main/v/vte2.91/libvte-2.91-0_0.52.2-1ubuntu1~18.04.2_amd64.deb  Redirection loop encountered
E: Failed to fetch http://192.119.223.46:80/data/011634d2c0cdf482/us.archive.ubuntu.com/ubuntu/pool/main/l/linux/linux-libc-dev_4.15.0-30.32_amd64.deb  Redirection loop encountered
E: Unable to fetch some archives, maybe run apt-get update or try with --fix-missing?

If I run sudo apt-get update --fix-missing, I get the same errors.
What does the "Redirection loop encountered" mean? How can I fix this error?
No problems with apt-get update. The sources.list file seems to be OK.

A: You are going to love this.
192.119.223.46 resolves to 192-119-223-46.client.mchsi.com.
mchsi.com belongs to Mediacom.
If you are a Mediacom customer it means they are hijacking your traffic and sending it somewhere it shouldn't go.
I would try a traceroute to us.archive.ubuntu.com and see where it diverts.
I would also try using a free DNS server like Google's 8.8.8.8 or Cloudflare's 1.1.1.1.

A: I just got this error.
I initially thought the message meant sudo apt-get update --fix-missinguntil I triedsudo apt-get upgrade--fix-missing

A: This worked for me

*

*Go to software and updates

*Under the download from drop-down menu,select other

*Choose best server

*After a while it will pick a server for you. Choose server and let it do the configuration.

*Then close and try to install, update or upgrade from the terminal.

Hope it helps


text:
SouthworldCulture April 2021Venezuela. The Indios Warao: the 'Boat People'.
Venezuela. The Indios Warao: the 'Boat People'.
Thursday, 1 April 2021 8:00
Category:	Culture April 2021
The Warao indigenous people inhabit the states of Bolívar, Monagas, Sucre and Delta Amacuro in the eastern part of Venezuela. The majority of them lives on the banks of the Orinoco River in the Delta Amacuro. This tribe, which is currently estimated to number about 50000, is the second-largest indigenous group in Venezuela,
after the Wayuu.
'Warao' is a name made up of two words: 'Wa' that comes from curiara, which means boat or canoe (this group's main form of transportation) and 'Rao' meaning people or owners, hence the meaning of the word 'Warao', 'owners or people of the curiaras'. The Warao have lived for centuries on the Orinoco Delta.
According to Warao mythology, in pre-cultural times the Warao lived in the sky, where a young archer 's shot went wide one day. While searching for his arrow, the archer, whose name was 'Jara Yakera' (Good Arm), found a hole and looked through. He saw lush and fertile land (Earth) and descending by a rope to the earth, he discovered an abundance of food. On his return he informed the Warao of his extraordinary find. The other Warao immediately decided to descend to Earth and live there, forsaking their sky world.
Warao Community (Photo: Luis Ovalles/C.C.)
The Warao of the Orinoco Delta refer to Kanobo ('great father') as the author of life and their protector, and 'Kanobo arima' is the name of the Wisidatu, a spiritual guide and the intermediary between Kanobo and the members of the community. Festivals are celebrated in honour of Kanobo by this indigenous group; during these ceremonies offerings such as yuruma (aru) and wina are made to the god and Jabisanuka and Najanamu (sacred dances) are performed in order to maintain good relations with the god and to be saved from diseases.
Kanobo communicates through dreams with the Kanobo arima. The 'yuruma' offered to Kanobo is prepared only by those women who already went through menopause, since the Warao believe that women experiencing menstruation may contaminate the yuruma and makes it impure. When the yuruma is ready, the Wisidatu puts it into a container which is placed under the altar dedicated to Kanobo.
Warao Indigenous Community settlement on the banks of the Morichal Largo river, Monagas.(Photo: Ricardo Ricardo/C.C.)
On the day of the offerings the Kanobo arima invites other Wisidatus and they gather at the Kanobo's temple where the ceremony is held. Before starting, the Kanobo arima smokes and invokes Kanobo and the spirits of other Wisidatus, and then the intercession for the community starts. Later the sacred dances Najanamu and Jabisanuka are performed.
Young or menstruating women cannot participate in the dance because they might contaminate the dance and make it impure, and that may cause Kanobo's anger and bring diseases among the members
of the community.
The dances that are performed in honour of Kanobo are considered sacred by the Warao, and they respect the different rules that these require. For instance, the different instruments used for the music that accompanies these sacred dances are used only for this purpose and are kept in a special container (torotoro). They can be only used by the wise men and the community elders, who are the healers and protectors
of the community members.
The Warao indigenous people believe that everything in nature has spirit, so there is a lot of respect for the jungle, the mountains, the stones which are believed to have spirit called maisikiri, Jobaji arao or other names. Therefore, the spirits of trees, rivers, those of the jungle,
are all greatly respected.
The elderly and wise men of the community are also very respected because they are the preservers of the community's ancestral culture and the owners of wisdom. The Wisidatu is a shaman that plays an essential role in the community; he is the intermediary between Kanobo and the community, he communicates with spirits, and is believed to be able to expel the evil spirits from the body of people. Some Wisidatus cure mainly the spirit of a person, while some others cure physical diseases.
Orinoco Delta. Warao family in canoe (Photo: Kate Palitava/C.C.)
Maintaining good relationship with spirits is important to the Warao people. There are rules that must be respected in order not to make spirits angry. For instance, before cutting a tree, the spirit of the tree should be asked for permission. Or when a new curiara is made, people must remember that they are not supposed to let a lady with menstruation board the boat, because the boat would be contaminated and become impure and the spirit of wisdom ('moyotu') would get angry. In the same way, the Warao people ask the spirit of the jungle for permission before cutting down trees to make room for sowing crops in order to obtain food. The wisdom of a people derives from their knowledge and traditions and the Warao communities are living examples of indigenous people who care, respect and protect their territories and the environment.
Johan Ramos
Honduras. The Pech people. "We belong to the earth and…
Colombia. San Basilio de Palenque. A place of resistance and…


text:
Cet article présente les résultats détaillés du simple dames de l'édition 1989 de l'Open d'Australie qui est disputé du 16 au . Ce tournoi de tennis est le premier de la tournée du Grand Chelem.

Faits marquants
Steffi Graf bat Helena Suková en finale de cette édition ; elle décroche à cette occasion son  du Grand Chelem et le  de sa carrière sur le circuit WTA.

En demi-finale, les deux protagonistes avaient respectivement battu Gabriela Sabatini et Belinda Cordwell.

Parcours

Résultats

Tableau final

Premiers tours

Première partie

Deuxième partie

Troisième partie

Quatrième partie

Notes et références

Voir aussi

Article connexe
 Palmarès du simple dames des Internationaux d'Australie

Lien externe
  Site officiel du tournoi

Navigation

1989
Australie Simple dames
Tennis en Australie en 1989
Tennis en janvier 1989

redpajama_set_name: RedPajamaWikipedia


text:
New job? Be careful not to take personal data with you!
Burnetts' information law solicitor Natalie Ruane discusses a warning issued by the Information Commissioner's Office regarding taking personal data with you when you leave for a new job.
The Information Commissioner's Office (ICO) has recently warned employees that walking off with personal information from their employer when they leave for a new job is a criminal offence. In September a para-legal who previously worked at Jordans Solicitors in Dewsbury was prosecuted for illegally taking the sensitive information of over 100 people before leaving for a rival firm in April 2013. The information was contained in 6 emails. The information included workload lists, file notes and template documents. However all of these still contained personal data about others.
James Pickles was fined £300, ordered to pay a £30 victim surcharge and £438.63 prosecution costs.
The ICO Head of Enforcement, Stephen Eckersley, said: "Stealing personal information is a crime. The information contained in the documents taken by James Pickles included sensitive details relating to individuals involved in ongoing legal proceedings. He took this information without the permission and has been rewarded with a day in court and a substantial fine."
"Employees may think work-related documents that they have produced or worked on belong to them and so they are entitled to take them when they leave. But if they include peoples' details, then taking them without permission is breaking the law."
This offence is punishable by way of a fine only of up to £5,000 in a Magistrates Court or an unlimited fine in a Crown Court.
You also have to ask what restrictive covenants relating to confidential information, Mr Pickles was also breaking at the time!!
Natalie Ruane
Natalie is a Partner and leads the Employment Law & HR team and specialises in education.
Published: Tuesday 18th November 2014
Categorised: Employment, Information Law


We do not give access to your contact information to third-parties when they displace advertisements. We do not share your telephone number or credit card information for use by other companies.
We give you choices and control over the collection and use of your Personal Information. You can, at any time update your Personal Information, remove your email address from our email list or remove your name from our newsletter.
We will act promptly to honor your requests to change or delete your Personal Information, but processing may take up to two weeks. Requests to remove Personal Information from marketing lists will apply to future mailings and future sharing of information.
We do not share email addresses you provide to us when you use the Wish List, eGift Card or when you contact us via email. The types of Personal Information collected about other people at pages like these may include the person's name and e-mail address which is used on a one-time basis to facilitate the communication on your behalf.


There are 11329 documents matching your search.
Use this 'Book: The Tale of Despereaux (upper elementary)' printable worksheet in the classroom or at home. Your students will love this 'Book: The Tale of Despereaux (upper elementary)'. Review some of the more difficult vocabulary from the 2004 Newbery winner, organized by chapters.
Use this 'Book: Al Capone Does My Shirts (upper elementary)' printable worksheet in the classroom or at home. Your students will love this 'Book: Al Capone Does My Shirts (upper elementary)'. Comprehension questions review Gennifer Choldenko's Newbery Honor novel about a boy growing up on Alcatraz Island.
Use this 'Writing Prompt: Sunflower simile (elementary)' printable worksheet in the classroom or at home. Your students will love this 'Writing Prompt: Sunflower simile (elementary)'. Have your students fill out the simile, and decorate a bulletin board with their writing, or trace and cut out the sunflower-shaped pattern for a shapebook.
Use this 'Spelling Level 2, unit 9 (elementary)' printable worksheet in the classroom or at home. Your students will love this 'Spelling Level 2, unit 9 (elementary)'. Worksheets to help learn these spelling words: quilt, shatter, clutter, slumber, clothes, chimney, special, nothing, against, important, comet, Uranus. Cloze exercise, word search, alphabetizing, missing letters and more!
Use this 'Book: A Picture Book of Christopher Columbus (elementary)' printable worksheet in the classroom or at home. Your students will love this 'Book: A Picture Book of Christopher Columbus (elementary)'. A six page book unit of activities that enhances vocabulary and comprehension for David A. Adler's picture book biography of the famous explorer. Includes character map and book report exercises in addition to vocabulary and comprehension work.
Use this 'Word Search: Mummies in the Morning (elementary)' printable worksheet in the classroom or at home. Your students will love this 'Word Search: Mummies in the Morning (elementary)'. Featuring vocabulary from Mary Pope Osborne's Magic Tree House book "Mummies in the Morning".


text:
September 13th Game Notes; Ducks @ Patriots
September 13, 2013 SomPatriots Preview 0
Bridgewater, NJ – Tonight the Somerset Patriots (43-23, 89-47) host the Long Island Ducks (31-36, 61-76) for the first game of a three game weekend series. First pitch is scheduled for 7:05 p.m. from TD Bank Ballpark. Download today's comprehensive game notes. Somerset is 13-6 against Long Island this year.
Final regular season series of the year begins tonight
Righty David Harden (10-2, 3.15 ERA) toes the rubber for Somerset while southpaw Bill Murphy (4-14, 5.31 ERA) will take the hill for the Ducks.
Postseason Party
The Patriots have made the playoffs for the first time since 2010. They will play the Sugar Land Skeeters in the first round of the tournament.
Winning Ways
The Patriots have won 39 of their last 51 games, 27 of their last 35, 24 of their last 31 games and 11 of last 16. Somerset has won eight of its last 10 series.
Eye On The Prize
The Patriots trail the Skeeters by 2.5 games in the second half Freedom Division race.
Best Year Ever
Somerset has totaled 89 wins which is the single season record for most wins in a season by one Patriots team. The 89 wins are also good for the second most wins all-time in a single season in Atlantic League history (16 years of existence).
Offensive Fire Power
The Patriots have scored the most runs in the Atlantic League (734). They also have the best team batting average (.279).
The Patriots are 76-16 when they score four or more runs. The Patriots are 13-31 when they score 3 or fewer runs.
Be sure to follow the Patriots on twitter @SOMPatriots for immediate updates. Listen the Patriots play-by-play voice Justin Antweil broadcast the game starting at 7:05 p.m. on 1450 AM WCTC or www.wctcam.com.
David Harden
TD Bank Ballpark
September 12th Game Notes; Bluefish @ Patriots
Patriots Win 90th Game Of Season


text:
University of Sheffield crest
Department of Molecular Biology and Biotechnology
Home > Molecular Biology and Biotechnology > News > Industrial biotechnology report launched in Sheffield
A global university
Menu iconMain menu
arrowThe University of Sheffield
arrowDepartment of Molecular Biology and Biotechnology
Industrial biotechnology report launched in Sheffield
The University of Sheffield has hosted an event held to launch the "Industrial Biotechnology Landscape Report: UK Industrial Biotechnology Framework and Strategy", which has been commissioned by the Networks in Industrial Biotechnology and Bioenergy (NIBB).
The Industrial Biotechnology Landscape Report: UK Industrial Biotechnology Framework and Strategy, sets out a series of recommendations designed to make the UK a world leader in Industrial Biotechnology and create a more sustainable and prosperous economy.
Industrial Biotechnology (IB) is the use of biological resources to manufacture materials, chemicals and energy. Commitments to reducing greenhouse gas emissions and the need to move towards a greener chemicals industry that is less dependent on fossil fuels are just two of the major challenges that IB can help resolve.
The new report assesses the importance of IB for the UK economy, provides a critical analysis of IB in the UK relative to competitor countries and identifies the opportunities and threats to the sector to produce evidence-based recommendations designed to strengthen the UK's IB position.
A major recommendation is the need for a credible long-term sector deal to support IB as part of the Industrial Strategy policy.
Jeff Green, Professor of Microbiology at the University of Sheffield and Director of Crossing Biological Membranes Network (CBMNet) - one of four networks to commission the report, said: "To keep pace with international competitors, the government needs to make clear its long-term commitment to industrial biotechnology.
"An encouraging signal would be to bring back the Industrial Biotechnology Catalyst fund that invested in translating the knowledge generated by the UK's academic research base and SMEs into new IB processes. But rather worryingly, IB was not prominent in the recent Industrial Strategy Green Paper with no acknowledgement of what it is, what it does, or what its future contribution to the UK economy and society might be."
At present IB companies employ 14,000 people in the UK, contributing £1.2bn in Gross Value Added to the economy, but it is estimated that the value of the global IB market could reach £360bn by 2025. To have a sustainable future the UK must take its place amongst the world's leaders in this growing sector of the economy.
Representatives from multinational companies (Akzo Nobel, BASF, GSK, AstroZeneca and Unilever), SMEs, academics from 15 universities and civil servants from the Department for Business, Energy and Industrial Strategy met to consider the report's findings and formulate the actions needed to ensure a bright future for UK IB.
Recognising the constraints imposed by feedstock availability, a focus on high-value products and a regional approach to modular manufacturing were amongst the recommendations discussed as a stepping stones towards a future sustainable circular economy based on IB.
Professor Dave Petley, Vice-President for Research and Innovation at the University of Sheffield underlined the role he believes the academic community has to play in the IB sector: "The University of Sheffield has a strong history of and commitment to collaboration. We have many examples of successful collaboration with industry partners such as Unilever, AstraZeneca, GlaxoSmithKline and Siemens, as well as many UK and overseas government agencies and charitable foundations.
"This event has brought together key players in IB who, like the University of Sheffield, are committed to using the Industrial Biotechnology Landscape report to influence policy and future funding allocations relating to bioscience and biotechnology. Through this commitment to collaborate we will deliver impact, through influencing policy, and making the UK's Bioeconomy one that plays a significant role in the UK's economic success."
The IB Landscape report was commissioned by four Networks in Industrial Biotechnology and Bioenergy (NIBB) and completed by economics consultants RSM.
The four NIBB who commissioned the report were CBMNet (Lead NIBB), BIOCATNET, P2P, C1Net.
For more information about the Industrial Biotechnology (IB) Landscape Report: UK Industrial Biotechnology Framework and Strategy report, please contact CBMNet Manager, Dr Jen Vanderhoven (jen.vanderhoven@sheffield.ac.uk).
NUMBER ONE IN THE UK FOR MEDICAL RESEARCH EXCELLENCE AND TOP FIVE FOR BIOLOGICAL SCIENCE
Research Excellence Framework 2014Arrow
@SheffieldMBB
Sheffield, S10 2TN


At Berloni Appliances we are family owned and operated that specialises in Kitchen & Laundry appliances.
Miele, Neff, Vzug, Ilve, Bosch, Smeg, Franke, Abey, Gessi, Barazza, Euro, Schweigen, Whispair, Liebherr, Samsung, Bertazzoni, Belling, St.George, Insinkerator, Interchange, La Germania, Vintec, Omega, Qasair, Saeco, Zip and more.
At Berloni Appliances being family owned & operated we put our own money into every single product we sell.
Because we specialise in Kitchen & Laundry Appliances we do extensive research into all of our brands to provide you the highest quality products available.
We have a motto "What would we buy for our own house?"
The answer to that is simple; the highest quality product to suits my needs & budget.
With this we will always guide you through the difference in products to let you make the correct final decision.
Berloni is known for its personal one-to-one customer service and the ability to meet each person's needs and also caters to the needs of builders, architects, cabinet makers, home/interior designers, decorators & developers - providing unbeatable deals. We guarantee it!
With our years of experience in designing kitchens we have a product to suit anyone's needs & budget. We also give you detailed and experienced layout advice.