Tim Blake Nelson in "The Ballad of Buster Scruggs," a collection of six stories by Joel and Ethan Coen currently streaming on Netflix. In "The Possession of Hannah Grace," when a cop who is just out of rehab takes the graveyard shift in a city hospital morgue, she faces a series of bizarre, violent events caused by an evil entity in one of the corpses.
The six tales in Joel and Ethan Coen's anthology film "The Ballad of Buster Scruggs" are nasty, brutish and short. Varying in tone but all set in the American West shortly before the Civil War, these films are virtuosic, capturing different aspects of this enduringly mythic time, held together by a unified vision of dark humor and brutality, and sparkling with profoundly beautiful cinematography.
The Coen Brothers are the most specific, assured and skilled people making films, or possibly anything else, right now. Everything they do is so precisely constructed and utterly idiosyncratic, that as you witness each moment, it is clear that nothing could look or sound any other way than it does, and it is brilliant.
The first section, which is about the titular Buster Scruggs, is absurd, slapstick and ludicrous, hilariously violent and unrealistic. Do not stop watching if you don't connect with this farcical singing cowboy story; it is merely laying the conceptual scene for what follows, as open hilarity gives way to more restrained and ironic humor, and the viewer transitions from giggles to grimaces.
In "Near Algodones" James Franco tries to rob a bank and meets another memorable performance from character actor Stephen Root, who serves Franco the first of many reversals of fortune. This tale is pithy and ironic, as Franco's cosmic luck switches back and forth with breakneck speed. In the Old West, death is never far away, and each story tackles a different character grappling with this constant reality.
Each of these tales is disturbing in its own way, but "Meal Ticket" will haunt you forever. Liam Neeson plays "The Impresario," a man who drives a charming wagon that opens into a little stage, and there, the unfortunate "Wingless Lark," a young man without arms or legs, delivers a series of moving orations, from the poem "Ozymandias" to the Gettysburg Address. Neeson feeds the man, Harrison, and puts his makeup on for him, and Harrison's vulnerability is palpable; he represents every person in this pitiless world, waiting for disaster he has not the smallest chance of preventing.
No less than Tom Waits plays a grizzled prospector in "All Gold Canyon," one of the film's more endearing characters, a man in a battle with nature and the elements searching for gold. "The Gal Who Got Rattled" is the longest and most developed story, aching and even romantic. Zoe Kazan plays Alice Longabaugh, a young lady heading west in a wagon train, after a simply hilarious scene in the boarding house where she and her brother reside. Her character is gorgeously realized, and the rhythm of this story stretches perfectly, creating a respite in the quick pacing.
Then, the film ends so appropriately with "The Mortal Remains," a dazzlingly verbal piece that feels theatrical, set almost entirely inside a stagecoach where five characters ride to a place called Fort Morgan. In some ways, this story sums up the different viewpoints of those that came before it, as three characters debate the nature of man while two undertakers sagely look on.
Every minute of these stories is brilliant — spectacular looking and mind bogglingly well-written. In the capable hands of Joel and Ethan Coen, Western and human tropes of masculinity, mortality, humanity, and spirituality are bent, reformed, and displayed with a sharp crackle of ill will and, always, indelible visual grace notes. They are the Impresarios, creating a work that is so wholly realized on every level, so complete and effective in its singular vision, that these six stories add up to an achievement that is wise, ridiculous, gorgeous, hideous, and contains some of the best onscreen monologues and original characters of all time.
"The Ballad of Buster Scruggs" is currently streaming on Netflix.


Ric flair is one of the iconic figures in modern wrestling. He has changed the way people perceive wrestling as it were and inspiring many upcoming figures to join wrestling. Since hanging his boots he left an indelible mark on the wrestling stage by winning many accolades and retired a folk hero in popular culture. After retiring Ric flair became a manager and couched his daughter to start wrestling. He is regarded as one among the greats and this was sealed when he was inducted in the Wrestling hall of fame .His alias that he used for most part of his career is the Nature Boy. He is probably one of the most decorated pro wrestlers in the history of wrestling as a career. One of the most notable attributes of Ric flair is the fact that during his hay days as a wrestler he used to be charismatic. He would capture the crowd with his signature move and charismatic catch phrase endearing him to millions of viewers around the world. He headlined major views in his career due to his increasing popularity enabling him to become one of the most sought after wrestler during his prime. He would then go on to clinch major titles with opponents that hugely favored to win.
He has a loyal following to this day .He has faced major opponents in the wrestling circle. He pioneered in clinching major titles that had just been unveiled much to the joy of his fans across the world. He coached and managed his daughter to clinch the women's title after retiring. His loyal fans always chanted one of his most notable catch phrase whenever he would walk into the ring. One of the major moments in his career is having to clinch the World Heavyweight Championship eight times. He is the only person that has been inducted at the hall of fame twice sealing his career as among the best in his trade. Go here ricflair.com to visit and get more info about Ric Flair.
Before hanging his boots Ric flair has inspired so many upcoming wrestlers to become champions and he is one of the most influential wrestlers of all time. On many occasions he was the Posta boy for the wrestling federation due to his popularity and that the fact that he had many followers. He has continued to inspire many people in and out the ring. His character is even better since no one is too big or too small for Ric flair as his fans would attest.


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Pens rookie Wilson to undergo surgery, will be out 'longer-term'
By Mike HalfordMar 17, 2016, 11:47 AM EDT
Emotional week for Pittsburgh freshman Scott Wilson.
Wilson, who earlier this week scored a two-year, $1.25M extension for his good showing this season, will be out of the Penguins lineup as he needs surgery on an undisclosed injury, head coach Mike Sullivan announced on Thursday.
Coach Sullivan: "Scott Wilson is going to need surgery. There is no timetable yet but it is a longer-term injury."
— Pittsburgh Penguins (@penguins) March 17, 2016
Wilson, 23, had five goals and six points in 24 games prior to getting hurt, averaging just over 10 minutes per night. He, along with fellow recent signees Bryan Rust and Tom Kuhnhackl, had done a nice job providing energy and a shot in the arm to Pittsburgh's forward group — part of the reason all three were rewarded with contract extensions at the same time.
With Wilson out, it's expected that Dominik Simon will get a more regular lineup spot. Simon, a fifth-round pick in 2015, has played in each of Pittsburgh's last two games — both wins — and registered his first NHL point, an assist, in Sunday's win over the Rangers.

redpajama_set_name: RedPajamaCommonCrawl


The magic is in the sharing not the doing.
It's not enough for Alton Brown to display his culinary skills. Nobody is watching to be convinced that Alton CAN cook. We want to know HOW Alton cooks, and we don't want any of the steps left out.
When I started blogging I wanted people to read my posts for their quality. I soon learned that people would not read to confirm the quality of my writing.
People want useful content that helps solve their problems.
share all the steps involved in a recommended process.
reveal the "secret sauces" from other bloggers. Here's Jay Baer's.
sight sources to provide more detailed explanations.
I like it when readers use my posts as a "how to" reference and not just a "drive by" read.
Cook a lot of dishes knowing viewers will only attempt a few.
Every time I write a popular blog post, I want the next post to be just as popular. But thats not realistic. Cooking shows know every recipe will not be equally in-demand. Viewers pick and choose the dishes that appeal to their tastes and interests.
Blogging requires serving up multiple posts (2x per week for me) and allowing readers to take their pick. The truth is you never really know which posts will go to the top of the menu and which ones will flop. The readers decide and they will always surprise you, so write with a "come and get it" mentality.
One successful entree does not mean repeat.
The #1 downloaded recipe from the first 180 episodes of "Rachel Ray" is Buffalo Chicken Chili. This dish still made the top 13 of in-demand recipes for 2013. But if Rachel tried to replicate this popular recipe on every episode how many viewers would keep tuning-in?
I learned the hard way not to fall into the trap of trying to replicate a popular blog post after one of my posts blew up on LinkedIn. For days after my one-hit-wonder I battled writers-block as I tried to write in the shadow of a successful post. Eventually I released another post that – sure enough – didn't do as well.
I try to normalize around those words each time I experience post-erratic success syndrome.
Your personality is just as important as the recipe.
Whether its the straight-laced wit of Mark Dacoscos (Iron Chef America) or Duff and the eccentric crew on Ace of Cakes, successful cooking shows marry great recipes with magnetic personalities.
While good blogging contains useful information and helpful "how-to's", you cannot blog with the disconnected feel of a technical writer. You don't have to be like the Muppet's Swedish Chef – all personality, no recipe – but you have to bring a humanness to your writing.
Liz Ryan talks a lot about loosing the zombie-like speak and using your "human voice." In this article Liz points out how we would think differently If Famous People Branded Themselves Like We Do.
I am still working on perfecting the art of a good title. It's strange but I usually write a post around an original thought or a plausible title. In a previous post I share how I capture those spontaneous thoughts and titles.
In the cooking world, Alton Brown is the master of show titles. See if you can guess what these episodes are about "Live and Let Diet" (1313), "Gills Gone Wild" (1215), "The Bulb of the Night" (409), "Wake Up LIttle Sushi" (906). Check your accuracy using the episode #.


The Catskills region in New York is a year-round getaway destination for outdoor enthusiasts, nature lovers and anyone looking for a peaceful respite. From cascading waterfalls and sky blue lakes to sprawling wilderness, vineyards and farmlands, the region is rich with natural beauty. You'll find a wide range of options when seeking lodging, food and recreation.
The Catskills region offers lodging options for every budget. It is home to numerous bed-and-breakfasts, ski resorts and affordable hotels and lodges. Many of the B&Bs are in historic homes and offer a gourmet breakfast with mountain views. If you plan to spend most of your time outdoors, save money by choosing a budget-friendly lodge or hotel like the Catskill Mountain Lodge in Palenville (catskillmtlodge.com). Consider taking your trip in spring or summer to take advantage of the lower off-season rates around popular ski areas like Hunter Mountain.
With the Culinary Institute of America (ciachef.edu) nearby, many Catskills restaurants are run by creative and competitive chefs utilizing local ingredients to continuously raise the bar of dining experiences. After a long day of skiing or hiking, stop by Dancing Cat Saloon & Catskill Distilling Company (dancingcatsaloon.com) to taste the region's locally distilled spirits, and stay for live music. The Old Grist Mill Restaurant (oldgristmillrestaurant.com) on Shawangunk Kill is a fine-dining restaurant housed in an 18th-century mill. Tony's Place on the Lake (no website; 6649 State Route 52, Lake Huntington; 845-932-8110) is a family-owned restaurant offering dining on Lake Huntington and a playroom for kids.
The Catskills region is home to more than 50 peaks that rise above 3,400 feet and attracts thousands of skiers, snowshoers, snow boarders and other winter sports lovers as early as late November. Hikers, bird watchers and campers have their choice of diverse trails and sites to explore from spring through summer. For climbers, there are eight climbing areas, including Devil's Kitchen, Stony Clove and Deep Notch.
Families, couples or friends looking for less intense activities have numerous options. Funtastic Family Fun Park (no website; Route 32, Cairo; 518-622-3330) and Ponderosa Ranch (no website; Route 32, Catskill; 518-678-9206) offer miniature golf, bumper cars, horseback riding, paddle boats and family go-carts. Couples can enjoy a sunny afternoon relaxing in the tasting room of the mountaintop micro-winery of Windham Vineyards (windhamvineyard.com).
LaJambre, Aurora. "Catskills Getaways." Travel Tips - USA Today, https://traveltips.usatoday.com/catskills-getaways-54552.html. Accessed 25 April 2019.


This entry was posted on Tuesday, November 7th, 2017 at 11:48 pm	and posted in Dakar 2018, Dakar Update.	You can follow any responses to this entry through the RSS 2.0 feed.
Is this you Bill? Going back to Dakar ?
Yes & yes. It's taken a while but I'm heading back with a good team riding a Husqvarna rally bike.


M. B. Kahn was an "Over and Under" sponsor for Lexington Medical Center's recent Aim for Clarity fundraising event. Proceeds support the LMC Campaign for Clarity to enhance access to early detection of breast cancer for area patients. Thanks to team members Michael Satterwhite, Sharif Aziz, and James Satterwhite, along with Herbie Hames with Criterion Healthcare, for taking part.


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Tag: fantasynovel
Book Review: The Starless Sea by Erin Morgenstern
November 13, 2019 November 13, 2019 ~ readingsumpton	~ 1 Comment
RRP: $32.99 AUD
A huge thank you to Penguin Publishing Australia for gifting me a copy of this incredible book in exchange for an honest review.
Zachary Ezra Rawlins is a graduate student in Vermont when he discovers a mysterious book hidden in the stacks. As he turns the pages, entranced by tales of lovelorn prisoners, key collectors, and nameless acolytes, he reads something strange: a story from his own childhood. Bewildered by this inexplicable book and desperate to make sense of how his own life came to be recorded, Zachary uncovers a series of clues–a bee, a key, and a sword–that lead him to a masquerade party in New York, to a secret club, and through a doorway to an ancient library, hidden far below the surface of the earth.
What Zachary finds in this curious place is more than just a buried home for books and their guardians–it is a place of lost cities and seas, lovers who pass notes under doors and across time, and of stories whispered by the dead. Zachary learns of those who have sacrificed much to protect this realm, relinquishing their sight and their tongues to preserve this archive, and also those who are intent on its destruction.
Together with Mirabel, a fierce, pink-haired protector of the place, and Dorian, a handsome, barefoot man with shifting alliances, Zachary travels the twisting tunnels, darkened stairwells, crowded ballrooms, and sweetly-soaked shores of this magical world, discovering his purpose–in both the mysterious book and in his own life.
"Are you lost or are you exploring?"
I have no words.
This was beautiful.
It was poetic and magical.
It was everything I could ever want in a book.
And it's easily my favourite book of the year!
The Starless Sea takes you on an incredible, vivid adventure. Like Alice falling down the rabbit hole, this book thoroughly immerses you in magic and wonder. I have already set a goal to read it again before the year is out so I can further understand all the small intricacies!
After Zachary Ezra Rawlins stumbles across a mysterious book in his university library, he is whisked off on an adventure that leads him through a magical painted door to a mysterious underground labyrinth filled with stories.
This book was gorgeous! It made me remember why I loved The Night Circus so much. Erin Morgenstern has a way with words. She is a poet. Honestly, my heart is so incredibly full after reading this book. The characters were beautifully written and complex, we got a perfect slow burn (and wonderfully queer) romance and the plot was layer upon layer of intricate detail that came together so beautifully in the end. It's the kind of book that has you intrigued the whole way through and as you read the last page it's as if all these pieces fall into place and you look up from your book and just stare in utter reverence at how incredible some story tellers are.
Now I understand that this book may not be for everyone. It can be a little "heavy"and as I've stated, there is a lot of intricate detail that some people may find a bit much. But for me, it was absolute wonder. This is a world I would very much like to be a part of!
Over all, I cannot fault this glorious book. It has everything I love in a story and I will read it over and over and over for the rest of my days. Go ahead, leap down the rabbit hole, step through the back of your cupboard, walk through that painted door. You won't regret it. All the stars in the night sky for this one. Utter perfection.
Book Review: The Order Of The Rose By Alysha King
June 24, 2019 June 24, 2019 ~ readingsumpton	~ 1 Comment
Rating: ★★★.5
"You possess a power as great as that of the false Empress. You do not understand it yet but you will come to learn its true nature. It is the only match to true evil. You doubt it, but you must embrace it or you will fail."
When Carey Lee discovers that not only is she a witch but also part of a rebel order, she figures it can't be any worse than the life from which she just escaped. Little is she prepared though for what lies ahead – an all-powerful empire and a murderous Empress, a seemingly impossible quest to rescue her family, and a power so dark that Carey begins to doubt her very existence.
The Order of the Rose is the first in a series of three novels to be known as the Rose Chronicles.
This was an interesting read for me. I am definitely no longer the intended audience, and being a fairly well read adult woman, at times it felt a little too young for my tastes. However, I think younger readers would find this action packed adventure/fantasy rather enjoyable.
The Order Of The Rose tells the story of Carey. A young teenage girl suffering from amnesia after a traumatic childhood event. Who, upon escaping from a horrible orphanage, joins forces with Ji and Kat, two of her oldest friends (she just doesn't remember them!) Together they are the last remaining members of the magical rebel Order Carey's grandmother founded to fight the evil, Empress Malevolence.
I found this a little hard to get into to begin with, but again, I believe this is because it is intended for younger readers. The pacing felt a bit rushed and was driven mainly by the three protagonists having seemingly random encounters with evil. This was frustrating as it felt like there was constant action and very little quiet moments in between for us to really get to know the characters. Having said that, this was justified further into the novel once more of the plot was divulged, but it meant that the first half of the book felt slow and wasn't as enjoyable for me.
The second half of this book however, was where it started to really shine. Carey had some really nice character growth and became a far stronger, more likeable character and the action seemed to become more intentional when it came to driving the plot forward. I really enjoyed learning about the world and setting, however it was a little confusing at first as I wasn't sure where or when the story was set until about half way into the book.
The characters all had some great growth! I adored Kat! She was extremely fierce, capable and just an all round badass. Carey could be a little unsure and frustrating at times, but I think this was justified considering she was thrown into the middle of an epic battle between good and evil, having absolutely no idea what she was or who she was up against. The trio's dynamic was also really fun and not unlike the Harry Potter series in some ways, which I really enjoyed.
Overall I liked this book! I think I would have liked it more if I had read it when I was younger. I may even go as far as to say it could be classed as more of a Middle Grade read. But taking that into account, it was enjoyable and was a fantastic starting off point for the rest of the series.


Previous gene expression studies have identified factors influencing population-level variation in gene regulation. However, these efforts have been limited to a small set of well-studied populations. By leveraging the high resolution of RNA sequencing and broad population sampling, we survey the landscape of transcriptome variation across a globally distributed set of seven populations that span a breadth of human genetic variation and major dispersal events. We assess differences in gene expression, transcript structure, and regulatory variation. We find only 44 transcripts that show significant differences in expression, likely as a result of the small sample size, but we find that 25% of the variance in gene expression is due to population differences. This is a larger fraction than previously observed, and it is likely due to the greater breadth of human diversity assayed in this study. We also find that population-specific variance is mostly due to transcription variability rather than the configuration of expressed gene products. Additionally, known common regulatory variants have similar effects across populations including those we study here. These data and results serve as a resource cataloging the wide array of gene expression regulation affecting population variation among diverse groups, improving our understanding of transcriptional diversity.
A central challenge in modern medical and population genomics is identifying trait-disposing genetic variants, interpreting their molecular consequences, and determining the transferability of their functional roles across individuals and populations. While genome-wide association studies (GWAS) have correlated an abundance of common and (increasingly) rare variants with disease, far fewer studies have pinpointed causal variants, discovered the biological mechanism of the association, or replicated their findings in different populations. Here, we build upon previous work using transcript abundance and splicing as model systems for understanding how population substructure can impact the genetic architecture of biomedical traits –. In particular, we focus on a set of populations that span the "Out-of-Africa" migration of anatomically modern humans using CEPH Human Genome Diversity Panel cell lines, for which we have collected an extensive 'omics profile described below.
Genetic studies of microsatellites panels and single nucleotide polymorphisms (SNPs) have shown a decrease in genetic diversity as a function of a population's geographic distance from eastern or southern Africa –. This pattern fits a serial founder effect model, but it remains unclear whether transcriptome variation follows this pattern and how closely genetic effects on regulation mirror human migration history. Previous work has shown that population bottlenecks reduce heterozygosity and are associated with an accumulation of damaging and loss-of-function variation which can impact gene expression , . However, further molecular work is needed to settle the controversy regarding demography and its impacts on the distribution of functional genetic variation among populations.
Gene expression studies within and between well-studied populations have been transformative in cataloging gene expression differences, expression quantitative trait loci (eQTLs) with different types of regulatory variants, as well as allele-specific expression (ASE) that underlie many disease associations , –. Technological advances in RNA sequencing and transcript assembly have also enabled analysis of variation in transcript structure and regulation of alternative splicing. For example, splicing ratios can differ between distant populations even in the absence of expression differences, and some population-specific splicing differences are involved in known disease-susceptibility genes that correspond with differences in prevalence , . Additionally, thousands of unannotated transcripts have been identified within populations , , highlighting the difficulty in distinguishing population-specific transcripts that are functionally relevant versus those that simply arise from noisy splicing . Elucidating how gene expression regulation and splicing are impacted by historical human migrations will aid functional interpretation of the genome and improve our understanding of the transferability and evolution of genetic regulation across populations.
This study aims to characterize regulatory, splicing, and expression differences via RNA sequencing across a global sampling of seven populations from the HGDP. We have also performed medium pass genome (∼8X) and high coverage (∼96X) exome sequencing of these individuals, enabling us to characterize genetic effects on transcriptome variation. These integrated DNA and RNA sequencing datasets are generated from the broadest points of human migration history yet sampled, and serve as a resource for future studies analyzing functional differences across populations.
To assess the molecular underpinnings of population level transcriptome diversity, we have sequenced the DNA and mRNA fractions of 45 lymphoblastoid cell lines (LCLs) from seven populations in the Human Genome Diversity Panel : Namibian San, Mbuti Pygmies of the Democratic Republic of Congo, Algerian Mozabites, Pathan of Pakistan, Cambodians of East Asia, Yakut of Siberia, and Mayans of Mexico (Figure 1). Five of these groups are descended from the ancient human dispersals out of Africa associated with serial founder effects . The populations in this study capture important differences in human genetic diversity resulting from early subdivision within Africa and subsequent serial founder effects into the Near East, back to North Africa , southern and eastern Asia and Central America.
Fig. 1. Collection sites for genome-, exome-, and RNA-sequenced human lymphoblastoid cell lines (LCLs).
LCLs were immortalized from the populations highlighted above, as described previously , and the genomes, exomes, and transcriptomes were sequenced. Founder effect and migration paths have been reproduced from  to highlight the breadth of human migration history across which these LCLs were sampled.
DNA sequencing was performed via paired-end 101-base pair Illumina sequencing (Methods). Total coverage per individual genome and exome was 8.1±3.3X and 96.5±11.0X (mean ± standard deviation), respectively. Additionally, 15.4M±0.5M read pairs per sample were generated via transcriptome sequencing performed on lymphoblastoid polyA-selected mRNA, and an average of 10.8±4.6 million read pairs per sample were properly mapped to the hg19 transcriptome (Table 1). Gene quantification performed through Cufflinks  detects an average of 9,141 known genes expressed per each individual cell line, which is consistent with previous observations .
We randomized library preparations and sequencing across populations, including approximately one individual per population in each lane of sequencing in order to ensure that expression differences were due to biological rather than technical variation. We also sequenced technical replicates for each sample by sequencing each library preparation twice per individual. We assessed the correlation between replicates and identified problematic samples as previously described . Briefly, we applied an optimal power space (OPS) transformation to expressed gene and transcript quantifications to ensure that all data points contributed equally to correlation measures, eliminating bias by low and high FPKM values. Pearson correlations between technical replicates were high (r = 0.915±0.034 (mean ± sd) for genes (Figure S1), r = 0.641±0.167 for transcripts). Higher correlations between replicates for gene versus transcript quantifications likely reflect the greater uncertainty in the deconvolution of the relative abundance of transcripts within a gene. Because reproducibility between replicates was high, we pooled reads across replicates and reassessed gene and transcript quantifications with Cufflinks. For each sample, we determined the median Pearson correlations (D-statistics) with all other samples. D-statistics were high overall (median D-statistic = 0.948 for genes, median D-statistic = 0.862 for transcripts, Figure S2). We identified two outliers, both within the San population (HGDP01029 and HGDP00992), and we removed these samples as well as the two remaining San samples from all downstream analyses.
To compare gene expression patterns across individuals, we first normalized our data. Exon and gene counts were quantified over regions annotated in UCSC known gene tables. Previous work has shown that the sample preparation protocol for RNA-seq introduces nonlinear, sample-specific effects that explain more than 50% of the variation in expression data , . These nonlinear effects can manifest as sequence-specific biases , which we accounted for via conditional quantile normalization (CQN) . This normalization strategy removed large distributional outliers (Figure S4) by accounting for non-linear guanine-cytosine (GC) content and feature length effects.
As previously observed, genetic variation clearly differentiates globally diverse populations ,  (Figure 2A–D). A tree generated via hierarchical clustering of FST distances (Figure 2A–B) shows a clear separation of sub-Saharan African populations and out-of-Africa populations. Additionally, principal component analysis (PCA) of autosomal single nucleotide polymorphisms (SNPs) in the HGDP dataset (Figure 2C–D) shows population-specific clustering  with these seven global populations separating within the first four PCs. Despite clear clustering among the selected populations at the genetic level, PCA of gene expression levels assessed via Cufflinks reflects high individual expression variability and shows no clear population clustering (Figure 2E–F). A formal test of this hypothesis is presented in the last subsection of the Results section, "Variability in expression and alternative splicing ratios," which also considers the impact of population labeling as a factor in gene expression differences among individuals.
Fig. 2. Analysis of genetic and expression divergence among individuals and populations.
A) FST matrix with 100*FST values shown in the upper half and B) tree generated via hierarchical clustering. C–F) Principal components analysis (PCA) of genetic (C and D) and expression (E and F) values. Genetic values are from exome variants, which were called from high coverage (96X) sequence data.
We next sought to identify individual exons and genes that show strong evidence of differential expression (DE) among populations. We used a negative binomial model for gene expression analyses (Methods) and incorporated a normalization offset term from CQN via edgeR  (Figure S3); we find that our model provides a good fit to the data (Methods, Figure S4). We identified 251 DE exons via generalized linear model with a false discovery rate (FDR) of less than 5% when comparing all populations (Table S1). Two examples of genes containing highly DE exons are shown in Figure 3 (expression of all individuals shown in Figure S5), both of which are involved in immune function and have some previous evidence for population-specific effects , . Figure 3A shows the expression of MX1 colored by population (FDR = 1.57%). MX1 is known to affect the immune response to influenza, the West Nile Virus, the avian flu, and other DNA and RNA viruses , . Additionally, LSP1 (lymphocyte-specific protein 1, Figure 3B, FDR = 0.87%) has been associated with breast cancer risk in Europeans. Interestingly, this signal did not replicate using admixture mapping in Latina women, perhaps due to differences in allele frequency among the GWAS and attempted replication populations . We also identified 44 differentially expressed transcripts at ≤5% FDR (Table S2). We used gene set enrichment analysis (GSEA) of ranked p-values to detect functional enrichment of differentially expressed transcripts . The following categories were enriched with a FDR≤5%: RXR and RAR heterodimerization with other nuclear receptors (q = 0.007, canonical pathway), IL 2 signaling pathway (q = 0.015, BioCarta), and Top 40 genes from cluster 7 of acute myeloid leukemia (AML) expression profile (q = 0.018, chemical and genetic perturbations) (Figure S6).
Fig. 3. Differential expression across human populations.
Bottom plots show exon positions indicated by rectangles to physical position scale. Red rectangles are differentially expressed exons. Upper plots show the median conditionally quantile normalized (CQN) expression values per population of each exon in horizontal lines. Diagonal lines connect each exon. Each exon corresponds one-to-one with the transcript structure shown below but have been scaled evenly to the width of the plot for ease of visualization. Population orders on the right correspond with the order of expression values of the last exon. A) Expression by population of the uc002yzh.3 transcript of MX1. B) Expression by population of the uc001lui.3 transcript of LSP1.
Allele-specific expression (ASE) can be detected as a read imbalance at a given heterozygous site; it has previously been shown to tag regulatory variants . To identify the degree to which allelic effects on expression vary, we compared ASE sharing among individuals for variants in the high coverage exomes. We define normalized ASE sharing as the number of shared significant ASE events (p<0.05) with at least 30 reads, normalized by sharing of SNPs that are heterozygous with at least 30 reads, regardless of presence or absence of a significant allelic imbalance. Reads were sampled to have equal counts in order to account for expression variability. There is a rapid reduction in normalized ASE sharing as the number of individuals in the comparison set increases (Figure S7A). That is, even when heterozygous sites are shared, most allelic imbalances are private to an individual. Some allelic imbalances are shared by pairs of individuals; rarely do three individuals in the set share an imbalance and very little sharing occurs across more than four individuals. We compared normalized ASE sharing across individual pairs and found similar levels of sharing within and between populations (Figure S7B). A potential explanation for this lack of ASE sharing among individuals is that the allelic state of the underlying causal regulatory variant tagged by the ASE exome site is acting in cis but in weak linkage disequilibrium, potentially with a rare regulatory variant.
In a previous study, Stranger et al. (2012) mapped eQTLs in eight populations from the HapMap3 dataset. To determine if the effects of these previously identified cis-regulatory variants can be captured in our more diverse HGDP populations, we compared ASE events in our dataset to previously discovered eQTLs  across populations. We hypothesized that if an individual is heterozygous for a previously discovered cis eQTL SNP (eSNP), and a significant ASE signal exists in the associated gene, then the allelic imbalance is more likely to be driven by the eQTL (see Figure S8 for a graphical representation of the model). We assessed the HGDP genotypes of eSNPs identified in HapMap3 and determined that there is a significant ASE enrichment within eQTLs associated with heterozygous versus homozygous eSNPs (p<2.2×10−16, Figure S9). This finding is consistent with our model and previous studies  and indicates that our measures of ASE are tagging shared regulatory variation between these studies. We also calculated an enrichment score similar to an odds ratio to determine how often ASE events are found in heterozygous versus homozygous eQTLs compared to the number of measured sites (Methods) for each HGDP and HapMap3 population. We observe an enrichment of ASE events in heterozygous eQTLs versus homozygous eQTLs consistently in all populations, but we do not observe a signal showing stronger effects in HGDP populations that are more closely related to the eQTL discovery population (Figure S10). This supports the previous notion that the effects of common regulatory variation are largely shared across populations with taggability depending on patterns of shared LD .
We next sought to determine whether regulatory events discovered within populations replicate more consistently in more closely related populations. Because of the limited sample size and structured populations in this study, de novo eQTL discovery is infeasible. We therefore assessed cross-population regulatory sharing using previously discovered eQTLs . We compared Spearman's rank correlation coefficient ρ2 values, a measure akin to variance explained, between our dataset and the HapMap3 study and find consistency between the associations (r = 0.22, p<2.2 * 10−16). The −log10(p) values across studies were also significantly correlated (r = 0.14, p<2.2 * 10−16). We next measured the associations between eQTLs identified in each population. We find that the effect sizes of eQTLs are significantly associated across most pairwise populations (Figure 4), independent of genetic divergence. The reproducibility of eQTLs is similar across populations, indicating that previously discovered common eQTLs reflect either the true causal SNPs or tag the causal eQTL due to similar LD at the locus (Figure S11). We also assessed the impact of similarity in allele frequencies between studies on the ρ2 values and find that eQTLs with similar minor allele frequencies (MAFs) between studies replicate better than eQTLs with different minor allele frequencies. As expected, eQTLs with high MAFs in one study and low MAFs in another study replicate poorly (Figure S12).
Fig. 4. Comparison between eQTL correlations (ρ2) discovered in HapMap3 vs replicated in HGDP.
* indicates p<0.05, ** indicates p<0.01, *** indicates p<0.001. ρ2 values in this dataset were filtered to the same minimum threshold as in the HapMap3 study for consistency.
Using the genome, exome, and RNA-seq resource described above, we characterize the completeness of current gene annotations as previously described . By pooling our dataset of 1.7 billion paired reads, we identify regions of novel transcription that lie outside of previously characterized gene structures. By calculating per-base global sequencing coverage and merging together continuous transcribed regions above our cutoff filters, we identified 445,091 total regions of putative transcription in our LCLs, 384,285 (∼86%) of which corresponded to annotated exons in Refseq, Ensembl, UCSC, or Gencode databases (Methods, Figure S13). Conversely, 34,555 regions (∼7%) meeting our minimum expression threshold did not overlap with known annotations (Figure S14).
When we filter regions expressed in at least one individual per population at greater than or equal to 1 RPKM, there are only a few hundred of these 34,555 regions expressed across all individuals in that population (Table S3). Additionally, we see that every novel transcribed region expressed ubiquitously in one population is also present in at least one other individual of another population. This result suggests that the vast majority of novel transcribed regions are not population specific, but can be found across multiple diverse human groups.
Previous work indicates that exonic splicing may vary significantly more than gene expression variability across species within the same tissue , . The majority of previous human transcriptome work has focused on expression and regulatory variability, leaving the degree of alternative splicing variation across diverse human populations relatively unexplored. To understand expression and splicing relationships within and between human populations, we measured the coefficient of variation, cv, in gene expression (standard deviation divided by the mean) and the variability in alternative splicing ratios (Hellinger distance to the centroid of the splicing ratios of each gene across all individuals in the population, ) using methods developed previously . We find that the cv and values for genes are highly correlated between pairwise populations (cv correlations are, on average, within [0.44, 0.67] between pairwise populations, p<2.2×10−16 for each comparison (Figure S15), and correlations are on average within [0.64, 0.82] between pairwise populations. p<2.2×10−16 for each comparison (Figure S16)). The relationships overall between cv and values do not reflect the genetic divergences seen between pairwise populations (Mantel test with 1,000 Monte Carlo repetitions between cv Spearman rank correlation distance matrix and FST gives ρ = 0.38, p = 0.16, and the same test between and FST gives ρ = 0.44, p = 0.14).
We next used established methods to assess the proportion of gene expression variation among individuals attributable to population identity . We find that population label, on average, explains 25.0% of the variation in gene expression among individuals (Figure 5A) for all genes expressing at least two transcripts. To assess significance for each gene, we used a permutation test reshuffling population labels among individuals and find that the p-value distribution is heavily skewed towards low p-values compared to the expected uniform distribution (Figure 5B). This genome-scale level of population stratification for gene expression is higher than previously seen by the GEUVADIS consortium , which reported ∼3% of the variance attributable to population label as a factor when considering populations of mostly European descent in the 1,000 Genomes Project. These results are perhaps expected given that the populations in our study span a greater breadth of human genetic diversity. We repeated this analysis comparing each population to all other populations and find that a smaller proportion of the variation is due to population-specific differences and that these differences do not follow the pattern expected by population divergence (Figure S17). We also decomposed population-specific variability into variability in overall expression levels as opposed to splicing variability via multiplicative model, which, as previously demonstrated , , accounts for differences in scales and units between expression and splicing metrics. We find that on average, variation in gene expression explains the majority (75.5%±22.3% (mean ± sd), Figure 5C) of population-specific variation, indicating that alternative splicing generally makes up the minority of population-specific variation within humans. We repeated this analysis comparing each population to all other populations and find consistent results (Figure S18). We next assessed differential splicing between pairwise populations. In Figure 6, we show a sashimi plot of a gene (ENSG00000183291.11, SEP15) with substantial differential splicing across all pairwise populations. Overall, we do not see evidence for differential splicing patterns consistent with population genetic divergence (Figure S19); this result is consistent with a minority of population-specific variance in gene expression levels explained by splicing variability.
Fig. 5. Analysis of variation in gene expression and splicing among individuals attributable to population labels.
A) Distribution of percent variance explained by population across n = 5,334 expressed genes with ≥2 transcripts expressed across all individuals. B) Empirical p-values for genes in part A. P-values were calculated by permuting population labels for individuals 100 times and comparing to true population labels. Dashed line indicates the uniform p-value distribution expected under the null hypothesis of no association between population label and expression. The output from the multiplicative model can be interpreted similarly to an R2 coefficient of a linear model. C) The contribution of gene expression in the variance explained by the population.
Fig. 6. Splicing variability across human populations.
Each sashimi plot shows the expression within an individual for a differentially spliced gene (ENSG00000183291.11, SEP15). The RNA-seq read densities supporting expression over the region as well as the inclusion and exclusion of exons are shown and line densities are proportional to reads supporting splicing events. The y-axis on each sashimi plot indicates the expression in log10 reads per kilobase per million reads (log10(RPKM)). The plots on the bottom show the transcript structure within the gene.
We have analyzed the transcriptome landscape from populations spanning the breadth of human genomic diversity. While other studies have characterized variation within and among populations , , this study provides a unique opportunity to discover regulatory drivers of expression diversity in serially bottlenecked populations throughout human migration history. The HGDP populations in this study were explicitly chosen to encompass a large geographic range that experienced varied demographic histories, and thus they provide unique insight into global variation in transcription. In addition to gaining an understanding of transcriptome variation in diverse populations, this study also enables the discovery of novel gene structures and provides a public resource for analyses of diverse human transcriptomes.
In this study, we have assessed population-specific expression variability, alternative splicing, and regulatory variation. We account for technical artifacts in our analyses, including GC content and feature length effects, which otherwise add nonlinear systematic noise to expression data. We show that we substantially reduce technical sources of variation from these effects in our data and obtain high reproducibility between sequencing replicates. We detect few differentially expressed exons, which is likely affected by the fact that we analyze cultured cell lines grown in a highly homogenous environment. Further, given our sample size per population, we are only powered to detect very dramatic differences in expression among populations. Using variance decomposition methods developed previously, we find that 25.0% of transcription variability can be attributed to population differences among the six we study here. A previous study that sought to detect expression differences between the CEU and YRI estimated that ∼17% of genes were differentially expressed across these populations . This estimate is quite comparable to ours. However, the estimates from both studies are substantially larger than those reported by the GEUVADIS consortium, which found that population labels accounted for only ∼3% of transcription differences among 462 individuals sampled from the European populations in the 1000 Genomes Project as well as Yorubans. One potential reason why our analysis produced estimates larger than GEUVADIS is that the European populations sampled there are more closely related to each other than the breadth of populations studied here.
Immunity genes as a whole are overrepresented in the set of differentially expressed genes across populations. This is highly consistent with the immune role of LCLs we study here. This finding is also consistent with previous work showing that natural selection may have favored different alleles in certain immune genes across human populations and that differences in autoimmune disease risks may be a side consequence of differences in these evolutionary histories , . The increased expression of immune genes in LCLs also improves our power to detect differences with respect to most other gene functions. Potential mechanisms for differential expression across populations include variation in cis and trans eQTL allele frequencies, environmental differences, and epigenetic differences.
We also measured the population-specific variance attributable to expression versus splicing and find that on average, 75.5% is due to gene expression differences. This result is consistent with previous findings in humans and indicates that, within tissues, splicing differentiates populations less than expression. While this finding is consistent with previous human studies , , it appears to be inconsistent with other cross-species work , . This suggests that splicing potentially plays a greater role on longer evolutionary time-scales. Additionally, the methodology used to assess splicing varies substantially between these studies; in this study, we have used variance decomposition methods relying on gene and transcript annotation data, which is more limited in many other species. In the cross-species studies, exonic splicing was measured via "percent spliced in" (Ψ), which may be affected by expression variability or other forms of transcript differences, such as those arising from alternative start sites. Further work on the efficacy of alternative splicing quantification methodologies would benefit future studies.
We also show that eQTLs that were previously identified across a wide range of human populations show allelic imbalances and replicate consistently across populations, but this replication is dependent on minor allele frequencies. Our results suggest that rare eQTLs within a population that are common in another population will likely have differing effect sizes. Given that the ∼1.2 million SNPs assayed in HapMap3 are common and therefore largely shared globally, we have only limited power to assess the effects of rare regulatory variants. As more transcriptomes are sequenced across diverse populations, we expect that rarer eQTLs identified in large population-based genome- and RNA-sequencing studies will identify more population-specific enrichment patterns.
This study provides the first analyses of transcriptome diversity from serially bottlenecked populations spanning the breadth of human migration history. In this study, we integrated genome, exome, and transcriptome sequencing data from LCLs that are part of the HGDP. This enabled us to assess regulatory drivers of global expression variation in serially bottlenecked populations across a large geographic range and different demographic histories. We find that population of origin accounts for ∼25% of variation in transcription. While we are powered to detect only large differences in expression among populations, genes involved in immunity are overrepresented in this set. Of the 25% difference in transcription explained by population of origin, expression differences accounts for three-fold more of the effect than do splicing differences. Further, the common regulatory variants we replicate here impact expression across broad geographic groups relatively uniformly and do not correlate with the degree of genetic divergence among populations. We look forward to larger studies spanning the breadth of human diversity that are better powered to detect additional population-specific effects and cellular mechanisms of global expression variation. Here, we analyze the total variance in expression and splicing explained by global populations, which, together with other studies, suggests a complex genetic mechanism for population level variation in transcription.
Total RNA was extracted from lymphoblastoid cell lines in four San, seven Mbuti Pygmies, seven Mozabites, six Pathan, seven Cambodians, seven Yakut, and seven Mayans from the Human Genome Diversity Panel using an RNeasy Mini Kit (Qiagen). mRNAs were purified using magnetic oligo-dT beads and randomly fragmented to 300–400 nucleotides in length. First-strand cDNA synthesis was performed using random hexamers and reverse transcriptase. This was followed by second-strand cDNA synthesis with dUTP via the dUTP strand-marking protocol . Illumina TruSeq adaptors were ligated to the ends of the double-stranded cDNA fragments followed by digestion with uracil N-glycosylase (UNG) to remove second strand cDNA. A 300–400 bp size-selection of the final product was performed by gel-excision, following the Illumina-recommended protocol.
Each individual was sequenced in a 7-plex library on an Illumina HiSeq 2000 producing 101-bp paired end reads. Lanes were assessed for multiple quality metrics including number of reads, read quality, and reads mapping to the human genome. Two San individuals failed sequencing quality control and so all four San individuals were excluded from further analysis.
Sample genomic DNA was extracted from lymphoblastoid cell lines. Exonic regions were enriched using an Agilent SureSelect XT 44 Mb All-Exon Capture Kit (v2) and sequenced on Illumina HiSeq machines.
Illumina sequencing reads were mapped to the human reference genome (hg19) using a standard pipeline informed by the 1000 Genomes Project . Briefly, reads were mapped and paired using bwa v0.5.9 . Duplicate read pairs were identified using Picard (http://picard.sourceforge.net/). Base qualities were empirically recalibrated, indels were realigned, and variants were called using the Genome Analysis Tool Kit (GATK) v1.6 . SNP calls that failed the Variant Quality Score Recalibration (VQSR) step were filtered out.
Exonic SNPs were annotated using the RefSeq database to identify synonymous coding variants. High confidence and high coverage synonymous variants were used to compute Weir & Cockerham FST values  for each pairwise population using vcftools (v0.1.11) .
Reads were mapped to the human reference genome (hg19) with bowtie-2.0.0 and tophat-2.0.4 split read mapping algorithms using the "-b2-very-sensitive" parameters . Reads were subsequently filtered to include only properly paired reads. This yielded between 12.1 and 44.8 million reads per individual (29.3 mean±7.9 s.d. million reads), which corresponds to 62.17±13.79% of the total reads per individual.
Exon and gene count estimates were created by using bedtools to count read overlap with known genes and exons from the UCSC "knownGene" table file downloaded on July 17th, 2012 for differential expression analysis. Raw exon and gene read counts were normalized through conditional quantile normalization, which reduces expression outliers by accounting for feature level GC nucleotide content and overall feature length .
UCSC knownGene tables were also used for novel transcript structure analysis because a larger collection of gene structures have been catalogued in this annotation set. For all other analyses, gencode v13 annotations were used, because they give one-to-one correspondence of transcript to gene annotation, enabling the Gonzalez-Porta methods to be used as they were developed.
Transcript level quantification was performed with cufflinks-2.0.2 and produced FPKM (fragments per kilobase of exon per million) estimates per transcript. Cufflinks uses a generative statistical model of paired-end sequencing experiments to derive a likelihood for the abundances of a set of transcripts given a set of fragments. The likelihood function can be shown to have a unique maximum, which Cufflinks finds using a numerical optimization algorithm. The program then multiplies these probabilities to compute the overall likelihood that one would observe the fragments in the experiment, given the proposed abundances on the transcripts .
In order to compare expression levels in this dataset with those identified in Stranger et al , we reran Cufflinks (v2.1.1) using the Gencode v13 annotations to get both gene and transcript quantifications. These expression abundances were subsequently used to quantify the relative importance of variability in gene expression and variability in alternative splicing to individual transcript variability.
Sequencing variants called from the differentially expressed and differentially spliced regions were annotated for a series of functional predictions, conversation scores, and RefSeq database annotations as described below. This was done in order to better assess the significance of genetic variants present in the data and their potential contribution or involvement in modulating gene expression, transcript splicing, and phenotypic variability. General annotations include information from: the NHLBI Exome Sequence Project allele frequencies; 1000 Genomes Project allele frequencies; publically available Complete Genomics sample allele frequencies; region and exonic annotations from both Ensembl and RefGene; and information about protein structure and function from the UNIPROT and INTERPRO databases. Conservation scores were also produced from the following algorithms: GERP++, SLR, SIFT, LRT, PHYLOP, and SiPhy based on 29 mammalian genomes –. Lastly, functional prediction annotations were produced from the following sources: FATHMM, MutationTaster, Mutation Assessor, LRT, PolyPhen2, and the RefSeq RefGene database , .
Methods to characterize regions of previous unannotated transcription closely followed previously described work  (Figure S14). In brief, for each base of the genome we calculated global sequencing coverage and split the genome into continuous transcribed regions. Expression of a region was defined as the maximum per base coverage of bases in the region. As in previous studies, we chose a threshold of an average expression level of 5×10∧-8 (or 0.05 reads/million) to consider a region expressed and merged together regions separated by less than 15 bp . Sample specific expression of these novel regions was then quantified by calculating RPKM of each region for each individual. For these analyses, we ran Cufflinks (v2.0.2) using the UCSC KnownGene tables downloaded on July 16, 2012 because there were fuller annotations than in Gencode v13.
ASE was determined as previously . Briefly, variants were called for all HGDP individuals in this project using high coverage, high quality exome variant calls generated according to the GATK best practices. Samtools was used to determine the number of reads that matched the reference and non-reference allele. Imbalance reference allele mapping bias was compensated using the per individual overall reference ratio within the binomial test.
Here, y is the count at gene g in individual i, β is the vector of population effects, x is the population label, o is the offset term from conditional quantile normalization, and ε is the error term. We perform an analysis of variance (ANOVA) comparing the null hypothesis of β = 0 to the alternative hypothesis of β≠0. In pairwise population comparisons, we computed genewise exact tests for differences in the means between the two groups of negative-binomially distributed counts.
eQTLs discovered in the HapMap3 populations were replicated in our HGDP dataset using genotypes derived from the exome sequencing variants and preliminary results for the full genomic variants (Henn & Botigue et al, unpublished data) for eQTLs outside the exome (Data Access).
The SRA accession number for the genome and exome sequence data reported in this paper is SRP036155. The GEO accession number containing the RNA-Seq data and gene/transcript expression matrices reported in this paper is GSE54308. Links to additional data (exome variant files, eQTL SNP data, FST matrices, gene/transcript expression quantifications, ASE tables, and eQTL data) and scripts are provided on an FTP site by the Stanford Center for Genomics and Personalized Medicine computing cluster located here: http://bustamantelab.stanford.edu/datasets.html.
45. WeirBS, CockerhamCC (1984) Estimating F-Statistics for the Analysis of Population Structure. Evolution (N Y) 38: 1358–1370.


The East Bay Vivarium is home to this Hypomelanistic Boa Constrictor along with many other reptiles. The Berkeley shoreline has many activities to participate in such as dog walking, bird watching, and biking.
Steps away from Berkeley's elegant Fourth Street shopping district is a different side of the city. Instead of strolling by upscale restaurants and shops selling jewelry, lingerie and iPads, explore exotic reptiles and public art near Berkeley's shores.
Visit the East Bay Vivarium, the art installation at the Amtrak station and scenic Aquatic Park.
Located at Hearst Avenue and Fifth Street is the East Bay Vivarium (1827-C Fifth St.), one of the nation's largest collections of reptiles, amphibians and arachnids. You can see snakes, frogs, lizards, turtles and even tarantulas up close.
Although the creatures are for sale, browsers are welcome. Get an eye-to-eye look at a rattlesnake, baby iguana, baby tiger reticulated python and more types of boas than you ever imagined.
Not ready to own a snake? You can invite a giant boa or a black-throated monitor to your next birthday party. The vivarium's Traveling Reptile Program will bring its animals to you, with programs adjusted for any age group.
The East Bay Vivarium is open 11 a.m.-7 p.m. Monday through Friday and 11 a.m.-6 p.m. Saturday and Sunday. For more information, go to www.eastbayvivarium.com.
When you leave the vivarium, walk south on Fourth Street, past Spenger's Fresh Fish Grotto and under the University Avenue overpass. This once dark and somewhat dismal spot has been made into an art venue for painting and poetry. "Mak Roote," a vast installation by muralist John Wehrle and poet Betsy Davids, adorns the walls. Look up at the concrete beams above the columns to see evocative lines of poetry while listening to trains rushing by.
Continue under the overpass to the West Berkeley Rail Stop and Transit Plaza. This was once Berkeley's shoreline. Enjoy the mural showing Wavy Gravy walking his fish, and also see a moving memorial to Scott Slaughter, who was killed crossing the tracks in 2007.
Continue south on Fourth Street or cut through at Brennan's, an old-fashioned hofbrau, (700 University Ave.) and look at the artwork honoring the Ohlone in the concrete panels in front of the restaurant. The rest of the block is mixed-use apartments.
At Addison Street, turn right and carefully cross the tracks (a designated crossing), continuing to Bolivar Drive. (This part of the walk is a little grungy.) You shortly arrive at Aquatic Park, with the graceful arc of the pedestrian overpass in front of you.
Constructed between 1935 and 1937 by Franklin Roosevelt's Works Progress Administration, the park is an excellent place to see shorebirds - egrets, herons, avocets, dowitchers, willets and lots of ducks. Look for black-crowned night herons roosting in the trees. The central lagoon was carved out of San Francisco Bay when the Eastshore Highway was constructed. Walking down Bolivar, you pass some structures, after which you can pick up the pedestrian-bike trail right by the water. The total distance around is about 2 miles, but for a shorter walk, just continue to Dream Land for Kids, a children's playground complete with castles. Adjoining the playground is the Butterfly-Hummingbird Ecology Garden.
The Frisbee golf course is very popular. Rowing, motorboating, canoeing and water-skiing are allowed, but fishing is prohibited. Volunteers from the nearby animal shelter walk dogs around the park.
The Berkeley Pedestrian Bridge opened in 2002 to allow bikers, walkers and wheelchair users to cross over Interstate 80 and get to the Berkeley Marina and Eastshore State Park. Once over the bridge, walkers and bikers can connect with an 8.5-mile path that runs along the bay from Emeryville to Richmond.
In 2009, two mammoth sculptures, "Berkeley Big People," were added, one on each end of the bridge. Created by artist Scott Donahue, the statues were controversial at first but have become accepted as part of the Berkeley landscape. The stainless steel, fiberglass, concrete and bronze sculptures are almost 30 feet high and celebrate Berkeley's activist history with fist-waving protesters.
Cross the bridge to get a better look at the artwork. On the other side, stop at the Sea Breeze Market & Deli for snacks and coffee.
By public transportation, take BART to downtown Berkeley. At the corner of Shattuck Square and Center Street, catch the 51B and get off at University and Sixth Street. Walk one block west to Fifth.
By car from San Francisco, take Interstate 80 to University Avenue (exit 11). Keep right at the fork, following signs for Frontage Road. Then keep left at the next fork onto Eastshore Frontage Road and shortly turn right onto Hearst Avenue. Turn left onto Fourth Street. There are free parking lots along Fourth Street. You can also park on Bolivar in Aquatic Park.


text:
Osiemnasty sezon amerykańskiego telewizyjnego dramatu medycznego Grey's Anatomy: Chirurdzy został zamówiony 10 maja 2021 przez ABC. Premiera odbyła się 30 września 2021. Sezon produkowany przez ABC Signature we współpracy z Shondaland oraz Entertainment One Television.

Odcinki

{| class="wikitable plainrowheaders" style="width: 100%; margin-right: 0;"
! style="background-color:#132B69; color: #f9f9f9; width:3%;" | Nr ogółem
! style="background-color:#132B69; color: #f9f9f9; width:3%;" | Nr w sezonie
! style="background-color:#132B69; color: #f9f9f9; width:17%;" | Tytuł
! style="background-color:#132B69; color: #f9f9f9; width:17%;" | Polski tytuł
! style="background-color:#132B69; color: #f9f9f9; width:14%;" | Reżyseria
! style="background-color:#132B69; color: #f9f9f9; width:14%;" | Scenariusz
! style="background-color:#132B69; color: #f9f9f9; width:14%;" | Premiera(ABC)
! style="background-color:#132B69; color: #f9f9f9; width:14%;" | Premiera w Polsce(Fox)
! style="background-color:#132B69; color: #f9f9f9; width:4%;" | Kod

|}

Obsada

Główna
 Ellen Pompeo jako dr Meredith Grey
 Chandra Wilson jako dr Miranda Bailey
 James Pickens Jr. jako dr Richard Webber
 Kevin McKidd jako dr Owen Hunt
 Caterina Scorsone jako dr Amelia Shepherd
 Camilla Luddington jako dr Jo Wilson
 Kelly McCreary jako dr Maggie Pierce
 Kim Raver jako dr Teddy Altman
 Jake Borelli jako dr Levi Schmitt
 Chris Carmack jako dr Atticus "Link" Lincoln
 Richard Flood jako dr Cormac Hayes
 Anthony Hill jako dr Winston Ndugu
 Scott Speedman jako dr Nick Marsh

Drugoplanowe postacie
 Abigail Spencer jako dr Megan Hunt
 Lynn Chen jako dr Michelle Lin
 Alex Landi jako dr Nico Kim
 Melissa DuPrey jako dr Sara Ortiz
 Peter Gallagher jako dr David Hamilton
 Jaicy Elliot jako dr Taryn Helm
 Johnny Rey Diaz jako Noah Young
 E.R. Fightmaster jako dr Kai Bartley
 Zaiver Sinnett jako dr Zander Perez
 Sylvia Kwan jako dr Mabel Tseng
 Robert I. Mesa jako dr James Chee
 Nikhil Shukla jako dr Reza Khan
 Kate Walsh jako dr Addison Montgomery
 Jason George jako dr Ben Warren
 Debbie Allen jako dr Catherine Fox
 Stefania Spampinato jako dr Carina DeLuca
 Greg Tarzan Davis jako dr Jordan Wright

Gościnne występy
 Debra Mooney jako Evelyn Hunt
 Kate Burton jako dr Ellis Grey
 Greg Germann jako dr Tom Koracick
 Jaina Lee Ortiz jako porucznik Andy Herrera
 Grey Damon jako porucznik Jack Gibson
 Barrett Doss jako Victoria "Vic" Hughes
 Jay Hayden jako Travis Montgomery
 Okieriete Onaodowan jako Dean Miller
 Carlos Miranda jako porucznik Theo Ruiz

Produkcja

Do swoich ról powróciły Kate Burton jako dr Ellis Grey i Abigail Spencer jako dr Megan Hunt, które ostatnio pojawiły się w piętnastym sezonie. Nowi członkowie obsady serialu, którzy zostali obsadzeni w drugoplanowych rolach w tym sezonie, to Peter Gallagher jako dr David Hamilton, czyli dawny znajomy matki Meredith, Lynn Chen jako dr Michelle Lin, E.R. Fightmaster jako dr Kai Bartley oraz Greg Tarzan Davis jako dr Jordan Wright. Fightmaster i postać dr Bartley są osobami niebinarnymi, będąc jednocześnie pierwszym niebinarnym aktorem i postacią występującą w serialu.

Przypisy

Chirurdzy (serial)
Seriale wytwórni ABC Signature
Filmy, których akcja toczy się w Seattle

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The Irish have always been visionaries. They have never been afraid to dream big. It was William Butler Yeats who said: "I have spread my dreams beneath your feet; tread softly because you tread on my dreams." Over the past few decades, the dream of a dynamic, prosperous, confident nation became reality. And today, despite grave setbacks, this dream is still very much alive.
But Yeats also said "in dreams begin responsibilities". And those responsibilities are the co-responsibilities of Ireland and Europe.
Yeats didn't say "in dreams begin responsibilities". He wrote "in dreams begins responsibility" as an epigraph in his Responsibilities collection, a line which he attributed to an old play. The commonly cited quote that Lagarde uses is a Delmore Schwartz book title.
This entry was posted in Europe and the world by P O Neill. Bookmark the permalink.
George Orwell referred to Yeats as an "occult fascist" which was about right. W.B. was certainly no progressive in the democratic sense.
When you think about it Yeats and Lagarde have quite a bit in common. In company with the likes of Aleister Crowley (aka Great Beast 666), Yeats was an initiate and leader of the magical organization the "Golden Dawn" which he joined in the 1890's. He was known by the magical name Daemon est Deus inversus. Lagarde is of course initiate and head magician of that other esoteric organization – the IMF.


text:
Q: Cypress: We detected that the Chromium Renderer crashed Currently I'm trying to execute a single test with Cypress:
I used: cypress run
I'm getting: We detected that the Chromium Renderer process just crashed.
And:
Fatal JavaScript invalid size error 169220804

#FailureMessage Object:  Electron Framework            v8::internal::SetupIsolateDelegate::SetupHeap(v8::internal::Heap*) + 16184648

I already tried with chrome and Electron, windows and Mac and the issue persists.
I also tried --headless.
The test is quite simple, I'm performing the login and checking the url change
Looking into it, I found that the browser is crashing after a couple of seconds loading the main page (after login was successful)
I double checked with other web pages and it's working fine, so it's related to the main page of my project.
The main page is working fine if I access it from Chrome directly.
Looks like it's a memory issue loading the page but never see something like this before.
Node version: 14,16
Os: Windows 11, MacOS monterrey
Cypress: 10.7.0, 9.7

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Our clients, came to our firm, for design ideas for their interior renovation. They wanted to update and refine their office. When it came to selecting flooring, our firm relied on Shaw Contract. By using differing patterns in the same color family, it gave the office a new and sophisticated look. Initial reactions from our clients were phenomenal. They loved the design! Their clients were impressed, too. Due to the simplistic, yet elegant design, this firm will impact client now and in the future.


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Building effective partnerships to improve birth outcomes by reducing obesity: The B'more Fit for healthy babies coalition of Baltimore
Robin Truiett-Theodorson, Stacey Tuck, Janice V. Bowie, Amber C. Summers, Jodi Kelber-Kaye
Obesity affects a large percentage of Baltimore City's population with repercussions on maternal health and birth outcomes. Approaches to ameliorate its impact must be comprehensive and include stakeholder involvement at all levels of influence including policy makers, service providers, and community residents. In this article, we examine the evolution of the B'more Fit for Healthy Babies Coalition in Baltimore, Maryland, with a specific focus on how the public health alliance was formed, the strategies employed, and how partners continually evaluated themselves. This study offers the opportunity to understand the extent and complexity undergirding the collaborative processes of community coalitions as they strive to find innovative solutions to major public health concerns.
Evaluation and Program Planning
https://doi.org/10.1016/j.evalprogplan.2014.12.007
Coalition building and assessment
Gender-based analysis
Geography, Planning and Development
10.1016/j.evalprogplan.2014.12.007
Dive into the research topics of 'Building effective partnerships to improve birth outcomes by reducing obesity: The B'more Fit for healthy babies coalition of Baltimore'. Together they form a unique fingerprint.
Birth Outcomes Business & Economics 100%
obesity Earth & Environmental Sciences 82%
Baltimore Medicine & Life Sciences 79%
Obesity Business & Economics 71%
Community Coalitions Business & Economics 62%
Public Health Business & Economics 59%
baby Social Sciences 58%
public health Earth & Environmental Sciences 54%
Truiett-Theodorson, R., Tuck, S., Bowie, J. V., Summers, A. C., & Kelber-Kaye, J. (2015). Building effective partnerships to improve birth outcomes by reducing obesity: The B'more Fit for healthy babies coalition of Baltimore. Evaluation and Program Planning, 51, 53-58. https://doi.org/10.1016/j.evalprogplan.2014.12.007
Building effective partnerships to improve birth outcomes by reducing obesity : The B'more Fit for healthy babies coalition of Baltimore. / Truiett-Theodorson, Robin; Tuck, Stacey; Bowie, Janice V. et al.
In: Evaluation and Program Planning, Vol. 51, 01.08.2015, p. 53-58.
Truiett-Theodorson, R, Tuck, S, Bowie, JV, Summers, AC & Kelber-Kaye, J 2015, 'Building effective partnerships to improve birth outcomes by reducing obesity: The B'more Fit for healthy babies coalition of Baltimore', Evaluation and Program Planning, vol. 51, pp. 53-58. https://doi.org/10.1016/j.evalprogplan.2014.12.007
Truiett-Theodorson R, Tuck S, Bowie JV, Summers AC, Kelber-Kaye J. Building effective partnerships to improve birth outcomes by reducing obesity: The B'more Fit for healthy babies coalition of Baltimore. Evaluation and Program Planning. 2015 Aug 1;51:53-58. doi: 10.1016/j.evalprogplan.2014.12.007
Truiett-Theodorson, Robin ; Tuck, Stacey ; Bowie, Janice V. et al. / Building effective partnerships to improve birth outcomes by reducing obesity : The B'more Fit for healthy babies coalition of Baltimore. In: Evaluation and Program Planning. 2015 ; Vol. 51. pp. 53-58.
@article{b40a30f4fa834ba28d14396ac7c4619b,
title = "Building effective partnerships to improve birth outcomes by reducing obesity: The B'more Fit for healthy babies coalition of Baltimore",
abstract = "Obesity affects a large percentage of Baltimore City's population with repercussions on maternal health and birth outcomes. Approaches to ameliorate its impact must be comprehensive and include stakeholder involvement at all levels of influence including policy makers, service providers, and community residents. In this article, we examine the evolution of the B'more Fit for Healthy Babies Coalition in Baltimore, Maryland, with a specific focus on how the public health alliance was formed, the strategies employed, and how partners continually evaluated themselves. This study offers the opportunity to understand the extent and complexity undergirding the collaborative processes of community coalitions as they strive to find innovative solutions to major public health concerns.",
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author = "Robin Truiett-Theodorson and Stacey Tuck and Bowie, {Janice V.} and Summers, {Amber C.} and Jodi Kelber-Kaye",
note = "Funding Information: "This publication was made possible by Grant Number CCEWH111026 from the Office on Women's Health. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the OWH, the Office of the Assistant Secretary for Health, or the Department of Health and Human Services." Publisher Copyright: {\textcopyright} 2014 Elsevier Ltd.",
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T2 - The B'more Fit for healthy babies coalition of Baltimore
AU - Truiett-Theodorson, Robin
AU - Tuck, Stacey
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AU - Summers, Amber C.
AU - Kelber-Kaye, Jodi
N1 - Funding Information: "This publication was made possible by Grant Number CCEWH111026 from the Office on Women's Health. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the OWH, the Office of the Assistant Secretary for Health, or the Department of Health and Human Services." Publisher Copyright: © 2014 Elsevier Ltd.
N2 - Obesity affects a large percentage of Baltimore City's population with repercussions on maternal health and birth outcomes. Approaches to ameliorate its impact must be comprehensive and include stakeholder involvement at all levels of influence including policy makers, service providers, and community residents. In this article, we examine the evolution of the B'more Fit for Healthy Babies Coalition in Baltimore, Maryland, with a specific focus on how the public health alliance was formed, the strategies employed, and how partners continually evaluated themselves. This study offers the opportunity to understand the extent and complexity undergirding the collaborative processes of community coalitions as they strive to find innovative solutions to major public health concerns.
AB - Obesity affects a large percentage of Baltimore City's population with repercussions on maternal health and birth outcomes. Approaches to ameliorate its impact must be comprehensive and include stakeholder involvement at all levels of influence including policy makers, service providers, and community residents. In this article, we examine the evolution of the B'more Fit for Healthy Babies Coalition in Baltimore, Maryland, with a specific focus on how the public health alliance was formed, the strategies employed, and how partners continually evaluated themselves. This study offers the opportunity to understand the extent and complexity undergirding the collaborative processes of community coalitions as they strive to find innovative solutions to major public health concerns.
KW - Coalition building and assessment
KW - Gender-based analysis
KW - Infant mortality
KW - Obesity
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JO - Evaluation and Program Planning
JF - Evaluation and Program Planning


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← Retro Television Reviews: Fantasy Island 2.3 "The Beachcomber/The Last Whodunit"
January Positivity: Consider It All Joy (dir by William Mings) →
Posted on January 3, 2023 by Jedadiah Leland
Derek Leigh (Maxwell Caulfield) is a former Special Ops agent whose son and wife are killed by a drug lord (Trevor Goddard) than Leigh helped to put behind bars. Inspired by his dead son's love of super heroes, Leigh puts on a purple rubber suit and learns karate from Master Yee (John Fujioka) while The Toymaker (Paul Bartel) supplies him with an arsenal of weapons. Calling himself the Jaguar, Leigh goes after the men who killed his family.
Caulfield wears an obviously fake mustache for the first half of the film so that he can shave it off when he becomes The Jaguar. The camera never stops spinning around. Most of the fights look fake and the exterior of Derek's house changes from shot-to-shot. Stacy Keach plays a high-ranking government official but doesn't bother to get a haircut or hide his pony tail. Linda Blair plays a cop and sounds like she sucked helium before filming her lines. I'm not sure what she was doing in the movie. She may not have been sure either. Prey of the Jaguar is a reminder of just how cheap and cruddy most super hero films were before Marvel took over Hollywood. Of course, Derek doesn't really have any super powers, beyond getting proficient at martial arts in record time. He is just wearing the outfit to honor the spirit of his dead son. That actually makes more sense than most of the Marvel origin stories. The movie itself was too cheap to work and the actors were so disinterested that they seemed to actively be trying to make sure that there would never be a Prey of the Jaguar 2.
The most interesting thing about the film are the opening credits, which reveal that this film was executive producer by the Wolf of Wall Street himself, Jordan Belfort.
This entry was posted in Film, Film Review and tagged David DeCoteau, Linda Blair, Maxwell Caulfield, Prey of the Jaguar, Stacy Keach, Trevor Goddard. Bookmark the permalink.
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Artwork of the Day: Naked Acre (by Owen Kampen)


This post contains some spoilers of the new Netflix movie, Bird Box. There is also some discussion of suicide and criminal depictions of the mentally ill. If these topics are upsetting to you, please do what is best for you to keep your mind healthy.
Since opening last week, Bird Box has had the most successful opening week of any Netflix film to date. With an award-winning cast led by Sandra Bullock, this psychological thriller has piqued the interest of more than 50 million Netflix users.
I won't pretend to be a movie critic here, but the motifs of mental health and suicide leveraged throughout the movie inspired me to share some thoughts.
On an ordinary day in northern California, unseen creatures mysteriously descend on Earth and drive people to suicide en masse upon mere seconds of eye-contact with them. Our tragic heroine, Mallorie (Bullock) navigates survival over the course of 5+ years with two small children in tow. They eventually flee to a safehaven located inside a school for the blind guided by birds who can inexplicably sense when the evil forces are near.
As an added twist, the mentally ill are affected differently than other people. Instead of suicide, escaped mentally ill prisoners are inspired by the demonic beings to hunt nearby men, women and children and force them to "look".
I can't help but sigh as yet another Hollywood movie wields yet another "criminally insane lunatic" as the antagonistic force. I'm disappointed that I have to discuss this point because the argument is as cliché as the trope it opposes. But I'll say it again for those in the back — criminal intent is not inspired by mental illness in most cases and the depiction of mentally ill individuals solely as criminals and murderers is wrong.
In the end, Bullock and her children reach the school for the blind where hundreds of people live in harmony for the foreseeable future. There has been no progress on defeating the evil forces. Wild murderers still run amuck. Everyone is hiding from their problems and no one seems interested in addressing them.
In a movie that uses suicide and mental illness as a cornerstone for the plot, I was disappointed there were no solutions explored during the 124 minutes runtime. But then I thought about how our society (in the real world) often approaches the treatment of suicide and mental illness.
There's so much we still don't understand about various mental illnesses and conditions. As I think back on the ending of the movie, the safehaven feels like the wet bandaid we often offer to people with mental illness. Even in the movie — when literally everyone on Earth is at risk of suicide at any moment — the surviving characters would rather ignore the problem in an attempt at artificial normalcy than fight back and prevent anymore unexpected deaths.
Overall, Bird Box feels like a missed opportunity to steer Hollywood towards a more productive depiction of mental illness and to comment on the treatment mental conditions in society to nearly one-third of Netflix subscribers. Unfortunately, Bird Box has kept eyes closed to these pervasive issues of mental illness.
If you want to talk to someone or are experiencing suicidal thoughts you can text the Crisis Text Line at 741-741 or call the National Suicide Prevention Lifeline at 1-800-273-8255.


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ALL NOVEL
CHINESE NOVEL
KOREAN NOVEL
JAPANESE NOVEL
COMPLETED Read Web Novel Completed Translation
Zhanxian
Chapter 514.1 - Demon Heart Magic Weapon
Chapter 514.1: Demon Heart Magic Weapon
The distance was still far, the other party was just at the peak Yuanying stage, and the scope of his spiritual awareness exploration wss far less terrifying than Yang Chen, so he didn't even know that his figure has fallen under Yang Chen's spiritual awareness exploration, he was just guided by the compass. While advancing, he beheaded the demonic fiends that kept appearing around him.
Yang Chen calmly put away the demonic qi formation which was a few miles away, and then walked directly in the direction of the person.
As he walked, the nine quite flying sword kept absorbing demonic qi. It won't take long for such a little journey, but it could absorb one thing. A little increase in the power of the demonic qi may be able to make the opponent fall into a state of confusion.
Within a few hours, the other party found Yang Chen's whereabouts, and the compass needle trembling clearly told him that the target of the trip, which was Yang Chen, was not far away and his spiritual awareness also found Yang Chen's figure.
However, this guy was a cautious guy. Even if he found Yang Chen's person, he still didn't dare to be completely sure, but he was cautious and released a formation flag with a stealth circle, wanting to get closer to confirm it carefully.
"Are you here to find me?" Yang Chen broke the silence directly and asked without waiting for him to get close.
"Grandmaster Yang?" The other party had never seen Yang Chen, but had only heard the description. Hearing Yang Chen's voice, he no longer concealed it, put away the formation flag and asked in a very calm tone.
"Yes!" Yang Chen confessed directly, and then asked, "How do I call you friend?"
"Don't be so troublesome, take out what Elder Hu Qianyi wants, and I will let you have your life!" The opponent, a master of the Yuanying stage, was naturally confident and domineering when confronted with Yang Chen, who was at the early Yuanying stage "Otherwise, there will be one more demonized cultivator in this world."
The black mist around Yang Chen couldn't be hidden from the other party's eyes at all, and Yang Chen hadn't planned to hide it. At least in the eyes of the other party, Yang Chen was now invaded by demonic qi and looks overwhelmed. He thought he had given Yang Chen enough face by saying so, and Yang Chen couldn't help but refuse this situation.
"Oh? What is it that Elder Hu wants?" Yang Chen asked with a smile.
"I don't know." The peak Yuanying stage master smiled and replied, without giving Yang Chen a chance to speak again, he directly instructed "So, hand over all your things and I will spare you!"
Yang Chen could still laugh now, at least in the eyes of the other party, Yang Chen should be crying. The fifth grade alchemy master was not a strong figure in this demon continent. Demonic fiends do not care whether you are an alchemist or an expert. Those who should be demonized are still demonized. Now wasn't Yang Chen in this crisis?
No matter that the cultivator was unhappy, Yang Chen's touch could be said to be handy. He deserves his luck, and all of Yang Chen's good things would be cheaper for him. This time, not only would he be able to find what the sect needed and make great contributions, but also could retrieve the things Yang Chen extorted from the sect.
Those things Yang Chen extorted from the Greatest Heaven Sect were by themselves a huge wealth. Whether or not they were returned to the sect, it means a huge benefit to the peak Yuanying stage master.
In fact, he didn't want to save Yang Chen's life at all. The reason why he said so much was to wait for Yang Chen to be invaded by the demonic qi more deeply, and to deal with him with less effort. As for failure, could such nonsense happen?
"If you want something, you'd better come and get it yourself!" Yang Chen stood in place, no extra action, just a faint smile and provocatively said.
"You don't want to accept my good grace! Perfect!" The cultivator grinned and summoned his flying sword without hesitation, and slashed towards Yang Chen from a distance. In any case, this was the demon continent, so he was careful not to make a big mistake, the cultivator still did a good job.
The demonic qi on Yang Chen suddenly soared and greeted the opponent's flying sword. Seeing this situation, the other party was stunned. Obviously he did not expect such a situation, but it was just a start, and he still controlled the flying sword to cut towards Yang Chen.
As soon as the flying sword touched the black fog, it was as if a burning fiery sword had been inserted into a cold pool, making a hissing sound, which made people feel creepy.
Even a dull person would understand that this demonic qi was not good. The Yuanying stage ancestor hurriedly recalled the flying sword and checked it carefully, but there was no major problem, so he was relieved.
"This kind of weird method, I think it's a demon sect's technique? Unexpectedly, Grandmaster Yang Chen, a dignified fifth grade alchemist, was a demon, so it is necessary that this old man eliminate the demon and defend the dao!" Under the circumstances, the other party did not forget to find a righteous name, he called Yang Chen a demon and said that he was righteous and awe-inspiring, and he was an expert in slaying demons and defending the dao.
"So much nonsense!" Yang Chen retorted and directly turned the black mist into a huge whip, and drew it towards the other party's head and face.
How could the dignified peak Yuanying stage master be hit by this attack method, the opponent's figure flashed, he had already escaped and his flying sword immediately counterattacked.
The black mist suddenly rose, and suddenly the space of several tens of meters was directly covered, and the peak Yuanying stage master was directly shrouded in it. Seeing this situation, the other party was horrified, and hurriedly offered a few protective magic weapons to protect his whole body and was about to quickly leave the black mist.
The prey that had entered the hub, how could Yang Chen let him escape? The nine quite flying sword could change its form, instantly turning into countless spikes, it stab towards the opponent wrapped in the center.
No matter what magic weapon or flying sword, slashing at the black mist has no effect. Who has ever seen smoke cut off with a sword? The black mist penetrated directly onto his skin.
As soon as he came into contact with the black mist, the other party felt a trance, and then distracted thoughts arose. He immediately noticed that it was not good, and hurriedly launched a pure heart technique to quickly suppress these heart demons.
It can't be helped but said that the cultivator was still determined after all. Although he was infested by demonic qi, as soon there were signs of heart demons, he quickly calmed down.
"Sure enough, it's a demonic method, evil creature, you will die!" Perceiving the abnormality in the black mist, the opponent was even more furious, almost taking out the effort to press the bottom of the box, regardless of the black mist attack, he was just attacking Yang Chen.
"It's a pity!" Yang Chen on the opposite side could only utter such a sigh. This nine quite flying sword, after all, it was not long after refining, and the demonic qi was insufficient. Otherwise, just the moment it was just now would be enough to trigger this guy's heart demon, and he would directly become like Li Tianxue, which was really a pity.
© 2018 BOXNOVEL. All rights reserved
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graduates-shortlisted-for-20000-entrepreneur-prize
article Press releases 2014 November
Graduates shortlisted for £20,000 entrepreneur prize
From the left, Luke Cuthbert (Art of Football), John Baldwin (Baldwins Accountants), Sam Wilson (Virtalent), Ellie Bekalo (Virtalent), Shaun Knight (Baldwins Accountants), Stan Karpenko (Give Vision)
A 'virtual assistant' website that links resourceful students with busy entrepreneurs has earned its Aston graduate founders a place at the finals of a prestigious start-up awards.
Ellie Bekalo and Sam Wilson's business, Virtalent, has been shortlisted to compete against two other companies at the finals of Baldwin Accountants' KickStart Entrepreneurs Awards. The winners will receive a £10,000 grant along with £10,000 worth of mentoring and accountancy advice from Baldwins.
The finalists will deliver a presentation pitching their business to a panel of judges in front of a 300-strong audience at the event, which will be held at Villa Park on 20 November. Both runners-up will be rewarded with £5,000 of mentoring and advice.
Virtalent, launched in July, matches highly skilled UK university students with small business owners, to carry out tasks ranging from graphic design to social media management, for a flexible number of hours per month.
Ellie, who graduated alongside Sam from the International Business and Modern Languages programme at Aston, said: "We are very excited to have been shortlisted for the final of such a great award. It would mean everything for us to win. Both the grant the grant and support and advice being offered would be invaluable as we look to raise interest and the first round of investment in Virtalent.
"We are very proud of our business and the ways in which it helps people. If you are an entrepreneur just starting a business, we can reduce the worry, cost and time involved in hiring permanent members of staff. If there is an area of their business an entrepreneur is struggling to develop, we can assign a student with specialist skills to help, leaving the business owner free to concentrate on the more value-adding aspects of their company."
David Baldwin, a partner at Baldwin Accountants, said: "The finalists will present an overview of their business and we want them to highlight the quality of their product or service, their future plans and the strength of their personalities. We will give first prize to the business which has spotted the best gap in the market and has the most long-term potential."
Ellie and Sam received support and guidance from the Aston-led Birmingham Skills for Enterprise and Employability Network (BSEEN) while setting-up Virtalent. The BSEEN team assist students and graduates who are founding businesses by offering mentoring, workshops, a business grant and workspace. The programme is part-funded by the European Regional Development Fund.
Baldwins Accountants is one of the Midland's largest independent, family owned accountancy firms, with eight offices across the region. Tickets to attend the KickStart Final gala dinner at Villa Park are available from: www.baldwinskickstart.com
For further media information, please contact Jonathan Garbett, Aston University Communications on 0121 204 4552 or j.garbett@aston.ac.uk.
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The automated machine learning team at Microsoft Research New England (Cambridge, MA) is looking for a Research Software Engineer to join us! Our goal is to automate the selection and tuning of machine learning models (e.g. deep neural networks), as well as intelligently automate other components of the ML/AI pipeline, such as data acquisition and cleaning. We tackle these problems by developing novel meta-learning probabilistic models. Our work powers the current Azure Automated ML offering and we are continuing to rapidly transfer technology developed in our lab to our partner product teams. In this role, you would be responsible for implementing research ideas in robust and production-ready prototypes, as well as planning and executing the transfer of these prototypes to our product partners.
Microsoft Research offers a thriving environment for cutting-edge, multidisciplinary research, both theoretical and applied, with access to unique data, an open publication policy, and close links to top academic institutions around the world. Microsoft Research New England is comprised of thirty full-time researchers, engineers and postdocs working in machine learning, statistics, computational biology, theoretical computer science, algorithmic game theory, economics, social media, cryptography, and mathematics. The lab is collaborative and interdisciplinary and is actively engaged with the local academic and tech communities.


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Сент-Иле́р (; Святой Иларий) — топоним и французская фамилия.

Топоним
 Сент-Илер (Алье) — коммуна во Франции, департамент Алье.
 Сент-Илер (Верхняя Гаронна) — коммуна во Франции, департамент Верхняя Гаронна.
 Сент-Илер (Изер) — коммуна во Франции, департамент Изер.
 Сент-Илер (Од) — коммуна во Франции, департамент Од.

Фамилия
 Сент-Илер, Дуайт (род. 1997) — тринидадский бегун.
 Сент-Илер, Карл Карлович (1834—1901) — русский зоолог, директор Петербургского учительского института.
 Сент-Илер, Катон (род. 1997) — тринидадский футболист, полузащитник.
 Сент-Илер, Константин Карлович (1866—1941) — русский учёный-гидробиолог, профессор.
 Сент-Илер, Луи (1766—1809) — французский дивизионный генерал, герой революционных и наполеоновских войн.
 Сент-Илер, Жан Анри Жом (1772—1845) — французский художник, ботаник.
 Сент-Илер, Изидор Жоффруа (1805—1861) — французский зоолог, сын Этьена Жоффруа Сент-Илера.
 Сент-Илер, Огюстен Франсуа Сезар Прувенсаль де (1779—1853) — французский путешественник и ботаник.
 Сент-Илер, Этьен Жоффруа (1772—1844) — французский зоолог, отец Изидора Жоффруа Сент-Илера.
 Бартелеми-Сент-Илер, Жюль (1805—1895) — французский философ, журналист и государственный деятель.

См. также
 Аббатство Сент-Илер

Примечания