text:
Final salute to a hero: Tens of thousands of NYPD cops bring Fifth Avenue to a halt as they honor fallen colleague, 22, who was gunned down in cold blood - along with rookie partner - in Harlem
Brigitte Macron says she's always been aware that the 'the French People didn't want her' when her husband Emannuel was elected and admits she has to be careful who she confides in because she 'talks easily'
Here we go again! Key US oil index sees prices slump by 10.5% as COVID 'Nu' variant sparks fears of fresh downturn, while Boeing stock also plunges as US bans travel from eight African countries
US oil prices sank by 10 per cent after enjoying near historic heights this fall
The drop comes as the Dow Jones reported a 1000 point drop on Friday
The hit to the national economy stems from the rise of the Nu COVID variant, which has restricted US travel to eight African countries
It brought a major blow to the airline industry and Boeing's stock
The variant is believed to be the most infectious version so far
By Ronny Reyes For Dailymail.Com
US crude oil prices fell by about 10 per cent per on Friday as global concerns grow over the Nu COVID variant and Joe Biden imposed new travel bans.
West Texas Intermediate crude futures, the US oil benchmark, dropped $68.85 per barrel on Friday after soaring to near historic heights in recent weeks.
It had begun the day at around $77 dollars.
The sudden drop in oil prices came as the Dow Jones Industrial Average reported a 1,000 point drop, or about 2.5 per cent decrease, the worst drop of the year. The S&P 500 and Nasdaq both reported a drop of 2.2 per cent.
The blow to the national economy arrived as the World Health Organization warned of the 'most infectious' variant of COVID-19 yet, named Nu or Omicron, in South Africa.
West Texas Intermediate crude futures serves as the benchmark for US oil prices. The cost per barrel dropped by about 10 per cent on Friday
The Dow Jones tumbled on Friday morning by more than 1000 points
There are no recorded cases of the variant in the US yet.
Most of the warnings are coming from the UK, where an unnamed senior expert from the Health Security Agency, the British COVID authority, has described the variant as 'the worst we have seen yet.'
The US has banned travel to South Africa and seven other neighboring countries after European nations began imposing travel restrictions, and travel and aerospace shares took a blow.
Canada has also done the same, banning anyone who has visited any of the eight countries in the past 14 days from crossing its borders.
Boeing reported more than a 6 per cent drop Friday afternoon, and Delta Air Lines and United Airlines, the only US carriers who had services schedule to and from South Africa, also took a dramatic hit, CNBC reported.
Delta's stock dropped by more than 10 per cent and United fell by 11 per cent. American Airlines also fell by nearly 10 per cent.
United had 87 nonstop flights to South Africa scheduled for December, and Delta had 35 set up.
Boeing's stock fell by more than 6 per cent while American Airlines tumbled by nearly 10
The airline industries took a blow amid the hectic holiday rush that saw million of travels
US WILL ban travel from EIGHT African countries in bid to... WHO calls new Covid strain Omicron: UN health chiefs label...
'Delta will continue to work closely with our government partners to evaluate any changes to U.S. policy,' the airline said in a statement.
The blow to the airlines came as millions of US travelers experienced long waits and delays at airports across the nation for the Thanksgiving holiday.
The instability of the current economy is only muddled further through the rise inflation - which is at 6.2 per cent, the highest it's been since 1990 - causing the price of basic goods and services to soar.
The Consumer Price Index rose 6.2 percent in October 2021 from one year prior - the highest it has been since 1990
The Consumer Price Index shows a rise in prices in every category from used cars, laundry equipment, furniture to food
The variant, B.1.1.529, is believed to have emerged in Botswana - from where there are no direct flights to the U.S. - and is also being found in neighboring South Africa.
The Nu variant named omicron was first spotted in Botswana before spreading to South Africa. Health experts believe it is the most infectious variant of COVID so far
Hong Kong reported a case after a passenger who had recently traveled from South Africa was found to be infected with the variant, and then infected another person while in the same hotel, quarantining.
Israel has also identified a case 'in a person who returned from Malawi,' with 'two more cases of people returning from abroad' placed in quarantine, the country's health ministry said Friday.
Dr. Eric Feigl-Ding, an epidemiologist and senior fellow at the Federation of American Scientists, said initial data from the variant was worrying and border restrictions should be imposed.
'Looks like vaccine evasion could be real with this variant,' he tweeted, pointing out that the two patients in Hong Kong who had the variant were both doubled-jabbed with the Pfizer vaccine.
Both Pfizer and Moderna have said they can quickly update their vaccines if they need to to take the new variant on.
The US had recovered from a spike in COVID-19 cases that began in late August due to the deadly Delta variant.
The nation reported about 34,522 new cases this past day and 394 deaths, according to Johns Hopkins University.
There were nearly 600,000 new cases reported this past week and more than 7,000 new deaths.
More than 74 per cent of eligible Americans, including kids 5 and up, have received at least one jab of the vaccine, with about 60 per cent of the population fully vaccinated against the virus.
Boeing, airline stocks tumble as new Covid variant spurs travel curbs
US oil prices plunge 10% amid fears of 'most infectious ever' Nu COVID variant


We love a creative theme, and sweet Madolyn loves helping mom plant flowers, so she decided to have a gardening birthday party! Seriously, this is the cutest idea ever! Also, it keeps the kids outside and doing something fun! For Madolyn's invitations we designed a gate fold invitation with an adorable outer pattern of tiny pink rubber boots, green gardening gloves, and pink watering cans! The liner was mint green and white striped with her monogram inside a floral wreath! The invitations were tied with pink grosgrain and were so perfect for this gardening birthday party! We designed custom coloring inserts that went inside small plastic pots for each child, with the elements from the invitation to color in! Each child potted a plant for themselves and then one also for the local children's hospital! Such a sweet idea and teachable moment! Madolyn's cake was custom made by Dessert Designs in Nashville and staff from the Clay Pot in Chattanooga came to help all the kiddos with their planting! Seed packets were also given out and the plants were tied with favor tags reading "take one to grow! xoxo, Madolyn". But our absolute favorite part we designed had to be the precious aprons we made for each child! They read, "a little dirt never hurt" and had the invitation motifs scattered as well! The staff from the Clay Pot wore large aprons with the imprint, "Flower Squad" which we also designed! This has to be the cutest gardening birthday party we have ever seen!


Welcome To ArtToCanvas specializing in fully stretch-mounted & ready to hang canvassed arts.
A canvas is the next best result to the original and establishes a great value to your published art.
Each canvas is brand new, custom side-finished, back-stapled and packaged immediately.
art by creating an original-like feel to it. (See same item with Brushstrokes added to title).
Here are 6 side finishes available at no extra charge.
All our packages will be leaving from our warehouses so there are no taxes, duties, or fees.
Please note that this is not a shelf item so please allow 5-7 working days for production time plus delivery.
so if you have another art or size in mind feel free to call us at 1.877.282.2007 weekdays 9AM - 5PM EST.
We at ArtToCanvas thank you for considering us for your canvas purchase.


BruIPO has issued a practice note concerning the term of protection and dates for renewal of re-registration patents based on EP(GB), GB, MY and SG patents.
By decision of 21 May 2014 in Civil Appeal No. 10 of 2013, the Court of Appeal construed the "date of the patent" in Section 115(4)(a) of the Patents Order, 2011 to mean the date of grant of the foreign patent on which the re-registration is based. As a result, re-registration patents are entitled to a term of 20 years from such date of grant.
Accordingly, in the new practice note, BruIPO indicates that effective 01 January 2015, re-registration patent owners have the option to calculate their renewal dates and fees payable using the date of grant as the base date instead of the date of filing of the foreign patent as previously.
We are currently reviewing this development and will be writing to all clients and associates with active re-registration patents on our records with advice as to the implications and options for future action.


To provide the ultimate results, race engines need all the unrestricted air they can get. Designed to maximize the performance of your race Xtreme component-equipped SE120R engine, this 62mm Throttle Body is ready to take on the track. The gigantic 62mm bore Throttle Body is extruded, precision machined to ensure a smooth air flow when combined with the Oval-Ported High-Flow Intake Manifold and port-matched Hurricane Cylinder Heads. Screamin' Eagle High-Flow Fuel Injectors P/n 27797-07 (sold separately) are recommended. All EFI models require ECM calibration (priced separately). For race application only.
Fits '06-later Dyna® and Softail® models and '06-'07 Touring models equipped with Screamin' Eagle Pro Hurricane CNC Oval Ported Cylinder Heads P/n 17799-10 and High-Flow Intake Manifold P/n 27082-10.


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Carriera

Club
Sundmoen debuttò con la maglia del Rosenborg in data 1º maggio 1978, in occasione del pareggio per 1-1 contro lo Strindheim. La squadra conquistò la promozione e, nel campionato 1979, fu capocannoniere della squadra assieme a Jørgen Sørlie, entrambi a 7 reti. Rimase in forza al Rosenborg fino al 1981, totalizzando 59 presenze e 24 reti in campionato. Nel 1982, passò allo HamKam, dove restò fino al 1985.

Note

redpajama_set_name: RedPajamaWikipedia


Indianapolis trade schools and colleges can prepare you to start taking advantage of the opportunities that this city has in store.
Circle City is where you will find the Indianapolis Motor Speedway—home to the iconic Indy 500 and arguably some of the best ice cream in the country. But that is certainly not all. With the city's declining unemployment rate and a growing workforce, colleges in Indianapolis can start preparing you for a wide range of promising fields, from the skilled trades to business to healthcare.
Indianapolis schools give you the freedom to choose from an array of current programs. Whether your interest lies in nursing, culinary arts, technology, or another exciting area, you can find a selection of innovative programs that provide both theoretical and practical training.
Get ready to enhance your potential. Locate nearby trade schools in Indianapolis by entering your zip code below!
This Midwest city is often underestimated, but Hoosiers are fiercely proud of Indianapolis. And for good reason. The city's Cultural Trail has recently caught the attention of major metro centers across the U.S. It is a $63-million innovative urban trail that spans eight miles and connects six major cultural districts. And it is only one of the city's many revered trail systems. It is also easy to break away from the city and travel to one of the amazing nearby state parks to get a healthy dose of nature.
But Indianapolis doesn't just offer a great quality of life. In 2017, Forbes named Indianapolis as the 21st best place in the country for business and careers.1 This is partially due to its diversified economy, which includes the healthcare, finance, and technology sectors. Overall, it is an affordable place to live and offers a relatively low unemployment rate. Residents are continually finding it easier to achieve in-demand careers here.
In the decade from 2012 to 2022, it is projected that there will be over 35,000 job openings every year across all sectors in economic growth region five (EGR 5). (EGR 5 includes the counties of Boone, Hamilton, Hancock, Hendricks, Johnson, Madison, Marion, Morgan, and Shelby.)2 Those jobs fall into many different occupational categories too, so there is a little something for everyone.
Take a moment to check out ten in-demand sectors in the Indianapolis region. (The job openings that are noted represent the average annual openings expected during the 2014-2024 period.)2 You are steps away from discovering the career path that can change your life for the better!
Food is big business in Indiana. Statewide, there were about 11,790 eating establishments in 2016. The industry employs 10 percent of the state's workforce. And in 2017 alone, Indiana's restaurants generated about $11.6 billion in sales.4 The culinary scene in Indianapolis and the surrounding region is strong and has many recognized restaurants. In fact, five Indianapolis chefs were semifinalists for the highly acclaimed 2016 James Beard Foundation Restaurant and Chef Awards.5 So the city is definitely recognized around the culinary world.
The culinary industry is projecting that there will be an average of 4,221 job openings annually. Although many of those openings are related to serving and bartending, the massive amount of growth can provide opportunities for those who are interested in becoming chefs or bakers and supervisors or managers. You could even decide to pursue your dreams of opening up your own food business.
Data from 2016 indicates that more than 33 percent of Indiana children, aged 10 to 17 years, are overweight or obese. And data from 2016 shows that over 67 percent of adults are overweight or obese.
As of 2014, the rate of invasive cancer is over 880 cases per 100,000 people.
A 2016 survey found that almost 19 percent of adults reported having fair or poor health.
As of 2016, over seven percent of adults had cardiovascular disease, and over 11 percent had diabetes. And almost 14 percent of the state's population was living with a disability.
In 2015, cloud-marketing company Emarsys decided to set up its North American headquarters in Indianapolis. The company is spending over three million dollars on expanding its existing downtown office and estimates that it will add almost 170 new jobs from 2015 to 2020.10 And that is just one company.
If a better career is what you are after, then why not transform that thought into reality? Indianapolis trade schools and colleges can help you do just that. So what are you waiting for? Finding programs that are being offered near you is as quick and simple as entering your zip code below. The career that you are dreaming of is within your reach!
1 Forbes, "The Best Places for Business and Careers," Indianapolis, IN, website last visited on April 18, 2018.
2 Indiana Department of Workforce Development, Hoosiers by the Numbers, Future Demand, website last visited on April 18, 2018.
3 Indiana Economic Development Corporation, website last visited on April 18, 2018.
4 National Restaurant Association (NRA), Indiana Restaurant Industry at a Glance, website last visited on April 16, 2018.
5 EatDrinkIndy, "Five James Beard Award Semifinalists for Indy," website last visited in April 18, 2018.
6 CareerOneStop, State Profile: Largest Employers—Indiana, website last visited on April 13, 2018.
7 The Henry J. Kaiser Family Foundation, Indiana: Health Status, website last visited on April 18, 2018.
8 Flaherty & Collins Properties, Largest Indianapolis-Area Ongoing Construction Projects, website last visited on April 13, 2018.
9 IndyStar, "5 Downtown Indianapolis Projects That Will Be Finished in 2016," website last visited on April 18, 2018.
10 Inside INdiana Business, "Silicon Valley of the Midwest Wins," website last visited on April 18, 2018.


We love fall. October is one of those months when we have every weekend booked. Probably the most anticipated event is the Fire and Rescue Open House, followed by the Fall Jubilee. We truly have the most fantastic fire department here in Manassas Park.


Unravel the full potential of your software!
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Test how all the servers of your software placed in various locations around the globe reacts to various load conditions.
It is essential that your software should be able to handle multiple users at a time. Load test is the key to find out that.
Limitation on number of threads or file description configured to the system can be found out.
Will your software survive in real life load conditions? Find out now!
Why do we recommend load testing?
Looking for cloud based Load testing? We can help you with that too. Our cloud based load testing services will ensure that your software pass the developmental life cycle as quickly as possible.
However, there has always been concern regarding cloud testing owing to numerous security flaws, fluctuating standards, data accessibility, inconsistent cloud severs, unavailability of skilled workers etc.
But not to worry! Our choice of operation when it comes to load testing is as effective as possible. Be it the equipment or the skilled workers is of excellent quality and will carry out the entire process with utmost care and accuracy.
After the initial conversation has been done. The product will be broken down to bits for analysis.
A defect report will be send to the client which detail about the issues, its severity and location.
The process will be carried out at regular intervals or the point where the client ask us to do test, in accordance with the subscription.


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December 27, 2020 - 1st Sunday after Christmas
Isaiah 61:10-62:3; Psalm 148; Galatians 4:4-7; Luke 2:22-40.
In the fullness of time God gives joy to those seeking the Consolation of Israel.
The readings for this Sunday visualize (1) the glorious public appearance of an awaited king with the prolific mother city, (2) the hallelujah choruses of heaven and earth, (3) the human dimension of the fullness of time, and (4) the consummation of hope for the faithful visionaries in Israel.
Isaiah 61:10-62:3.
The Prophetic reading opens with the exultant cry of a royal figure who speaks on behalf of true Israel.
God in God's own person has dressed this speaker in "garments of salvation" and a "robe of righteousness." This attire is appropriate to the joy and delight of a royal wedding, a time when the groom wears the most glittering headdress and the bride the most luxurious jewels. This glory in the social and political world is like the outbreak of new growth in fields that are fertile and blessed, and like the luxuriant plantings of well-watered and carefully tended gardens (verse 11).
As this figure representing Israel is gloriously clad before the nations, so Mother Zion will be revealed to the nations as vindicated from past woes (62:1-3). For her too, the headdress of royalty will glitter and shine as the Lord puts his arm around her (as it were: "…in the hand of the Lord,…in the palm [literally] of your God,"62:3). Zion and Israel, marvelously attired, are re-united in God's loving care for wife and son. They will be the ornaments of the time of fulfillment.
Such is the prophet's vision of the consolation of Israel.
As if beholding the blinding glory of the royal coronation, the Psalm breaks forth in a tumult of Hallelujahs! (Every occurrence of "Praise …" in the English versions is a translation of the Hebrew hallelu, the plural imperative. In hallelu-jah, the jah is the shortened form of the divine name Yahwéh.)
The psalm drives exuberantly through all the reaches of heaven and earth to find entities and creatures to summon to Praise!
In verses 1-6 the heavenly realms are called upon at large and in detail to hallelu the Lord. The poet follows the cosmic structure of Genesis 1 and of Psalm 104, so these heavenly powers both extend beyond and encompass what human eyes can see. After all the unseen heavenly things are summoned, the call goes out to all the stuff more familiar to the human eye.
As for the earthly realms, their summons to praise (verses 7-12) begins with the exotic creatures of the deep, then goes on to the mysterious places of the sky and the distant horizons with their storehouses of all kinds of weather. After summoning the mountains, trees, and the animals, both wild and domestic, humans are addressed: the mighty of the earth, but also the ordinary young men and women. Let them all hallelu the Lord because of his glory, but also … also because "He has raised up a horn for his people…for the children of Israel who are close to him" (verses 13-14).
Thus, as the climactic—and almost add-on—thought, the realms of heaven and earth are called to rejoice in something special for Israel. Because of this newly-revealed glory for Israel, all the world is called to hallelu-jah!
Galatians 4:4-7.
The Epistle selection is part of a rather complex theological discussion, but its pertinence to the Sunday after Christmas stands out in the following clauses (NRSV translation):
· "when the fullness of time had come…"
· "God sent his Son, born of a woman…"
· "born under the law, in order to redeem those who were under the law…"
The "Fullness of Time" has its meaning in reference to Israel's covenant history with the Lord, ranging through the promises to Abraham, Moses, and David, and the prophetic messages of Isaiah, Jeremiah, and the visionary Daniel.
The phrase "born of a woman" has echoes of the prophecy in Isaiah 7:14, but primarily affirms that God's care reaches its embodiment at a fully human level.
The phrase "born under the law" insists that salvation under the New Covenant is first of all for Israel, coming as the fulfillment of all God's patient and yearning care for that troublesome and beloved people. Whatever else Paul had to say about his fellow kinfolks, this priority of Israel in God's plan of salvation is steadily maintained.
The most gracious and endearing presentation of the Consolation of Israel is in this Gospel reading. It is the narrative of the aged ones who have waited so faithfully and persistently to see the salvation of the Lord, the righteous Simeon and the dear prophetess Anna.
The passage is at pains to make clear that Jesus' birth was fully in accord with the laws of Moses. (Strictly speaking, two separate rituals are combined here, the purification of the mother after birth, Leviticus 12, and the presentation of the male firstborn, Exodus 13:2, 11-16.) From this viewpoint, Jesus was fully an Israelite. He was duly circumcised on the eighth day after his birth (verse 21, just before our reading), making him a son of the covenant of Abraham (Genesis 17:9-14). Then, forty days after his birth, he and his mother were brought to the temple for the "purification" and the redemption of a firstborn son.
In the logic of the sacred rules, the firstborn belonged to God until the father made a sacrifice to redeem it and allow it to live in the ordinary world – an action referred to the sparing of the Israelite firstborn at the time of the exodus (Exodus 13:14-15).
Though Jesus was fully an Israelite, he was a poor one—economically speaking. The sacrifice presented by Joseph and Mary for her purification was two pigeons, the sacrifice made by the very poor who could not afford a sheep (2:24, referring to Leviticus 12:8).
It was while the parents were engaged in the details of fulfilling the law of Moses that Simeon and Anna found them. By the ordinary work-a-day folks, crowded and busy in the temple precincts, these two old folks must have seemed strange characters from another age. In the evangelist's view, however, they are heirs of Israel's true hope for its time of fulfillment.
In a poignant moment of prophetic insight, Simeon foresees Jesus' destiny and the pain that the mother is yet to know. Speaking to Mary he says, "This boy is assigned to be the cause of the falling and rising of many in Israel and to be a sign that generates opposition... And a sword will pierce your innermost being too" (verses 34-35, CEB).
So, true Israel, in its ancient wisdom, anticipates the agony and disturbance that yet lie between the coming of this little child and the mystery of God's final salvation!
January 6, 2021 - Epiphany of the Lord
January 3, 2021 - 2nd Sunday after Christmas
December 25, 2020 - Christmas Day
December 20, 2020 - 4th Sunday in Advent

redpajama_set_name: RedPajamaCommonCrawl


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Home Match Highlights Lionel Messi scores for Barcelona, Diego Maradona tribute
Lionel Messi scores for Barcelona, Diego Maradona tribute
Roy Nemer
Lionel Messi scored for Barcelona in their 4-0 win vs. Osasuna and had a tribute celebration to honor Diego Maradona.
The world football has had all sorts of tributes for Diego Maradona but perhaps the best one coming from Messi himself. In what was Messi's best match in months, he would score a great goal, the last one of game. But it was Messi's celebration which stole the show.
Messi would celebrate wearing a Newell'a Old Boys shirt with the number 10 on it, the same one worn by Maradona himself while at the club.
Messi dedicating his goal to Maradona.
🇦🇷 ❤ pic.twitter.com/YAwa9LmRSe
— Classic Football Shirts (@classicshirts) November 29, 2020
MESSI WHAT A GOAL pic.twitter.com/qeUdG0Tqr2
— MessiTeam (@Lionel10Team) November 29, 2020
Previous articleDiego Maradona passes away at 60 years of age
Next articleFormer Argentina coach Alejandro Sabella in poor health, in hospital
Mauro Icardi scores for PSG in 2-1 Trophée des Champions win vs. Marseille
Angel Correa scores for Atletico Madrid in 2-0 win vs. Sevilla
Mauro Icardi goal and assist for PSG in 3-0 win vs. Brest
El Diego Fan November 30, 2020 At 10:26 pm
Nice gesture from Messi.
That's what I expected him to do for his idol.
cox4 November 30, 2020 At 4:08 pm
https://www.youtube.com/watch?v=JoXH6LAlB2Q
funny moments with Diego 🙂
2010 world cup.
Rohandip Samantha el Redondo . November 30, 2020 At 2:03 pm
Recent reports are showing that dario sarmiento has been sold to Manchester City.
Is it true guys?
olive_majestic November 30, 2020 At 6:04 pm
Ive heard this as well. Not sure if it's confirmed
Cleanball November 30, 2020 At 8:17 am
Rumors on the possibility of swap between Mauro Icardi (PSG) with Dybala ( Juventus ); not an ideal bargain as both need to leave the club and start the new atmosphere
sergio November 30, 2020 At 8:42 am
PSG are also looking at sending Paredes to Inter.
Liverpool and Barcelona are looking at Garay as a short term solution given he is fit.
Cleanball November 30, 2020 At 8:32 pm
If Barca can't get Eric Garcia, then Garay will get the chance as Liverpool also eyeing him but it will much better to join Barca
Batigol November 29, 2020 At 11:18 pm
Anyone know if that Newell old boys shirt that was worn by Messi, was given by Diego to Messi?
Because the shirt looked old, it will be so much more meaningful if that Newell old boys shirt was actually worn by Diego, and was given to Messi as a gift.
Dfox1942 November 30, 2020 At 12:29 am
It was Maradona's actual shirt, believe it or not.
Anuparno November 30, 2020 At 2:35 am
Yes it was given by Maradona to messi
Richard November 30, 2020 At 10:44 am
And both were 33 years when they scored
Maradona's actual shirt? Awesome!
It shows Messi has treasured his shirt.
Magic touch of masterclass! The best player of the 20th Century and 21st Century scoring the exact same goal. It was written in the stars. zit will go down as one of Messi's most iconic celebrations. This is football and ultimate tribute
Richard November 29, 2020 At 3:59 pm
Beautifully done by Messi. The goal was a copy of the first goal Maradona scored for Newell's. Young Messi was watching that day. Today the stars aligned.
Well, Luka Romero scored his first professional goal at the age of 16 and things couldn't get any better. He celebrated it in the same manner and passion as messi did when he scored back one some 15 years ago. As for now, things are going pretty well for Romero. The club didn't hurry with him and gave him time to develop. And as things stands right now, he looks like another gem who's following in the footsteps of Lionel Messi. I do not expect him to reach such staggering heights quickly like messi, but for now, he certainly looks like the best 16 year old wonderkid coming out for us, Argentinians.
Hope he develops well.
Luka Romero scores in second division of Spain, and Barcelona now have lost Lenglet on top of Pique, Araujo, Umtiti, Roberto, and Busquets. Mingo could get a chance. A second note on Maradona, it is great but expected to see Messi pay homage to him. It is truly heartbreaking to experience this when we didn't hardly expect it. This is one of those situations where you can't just send prayers to "his family". You need to pray for the entire millions of Argentinians and the football world, plus Maradonas compatriots. All of these people are almost just as devastated as his family. How many people he touched with his unparalleled talent and success is astonishing.
https://www.youtube.com/watch?v=8NI5oTTW_is
GRACIAS D10S !!!
shOvwar November 29, 2020 At 9:38 pm
Amazing Video 😢😊
Gracias Amigo.
waveride November 30, 2020 At 3:26 pm
Cox4 my friend, the official video of "La vida tombola", the song that Manu Chao has written for Diego is also amazing. You most propably have seen it but if you haven't it certainly worth a try!
Dfox1942 November 29, 2020 At 12:58 pm
Was watching the game and I knew he was going to score and perform a salute to Maradona.
Hopefully many goals will follow for club and country.
Santi-aires November 29, 2020 At 1:12 pm
Great game too. Key pass for first goal. String of one-two's to move ball into position for the second. And set up Trincao for what should've been the fifth


Who made this one in the black bear?
This grasshopper I mean-the one who is flung herself out of the grass, the one who is eating sugar out of my hand, who is moving her jaws back and forth instead of up and down who is gazing around with her enormous and complicated eyes.
Now she lifts her pale for arms and thoroughly washes her face. Now she snaps her wings open, and floats away. I don't know exactly what a prayer is. I do know how to pay attention, how to fall down into the grass, how to kneel down in the grass, how to be idle and blessed, how to scroll through the fields, which is what I have been doing all day.
Tell me, what else should I have done? Does everything die at last, and too soon?
Tell me, what is your plan to do with your one wild and precious life?


text:
Q: Issue to show data from relational database tables in rails I am a newbie in ruby on rails it's also my first ruby application.
I want to show data from two tables these are
applied_jobs table: it contains the below columns:

applied_jobs_id  |  jobseeker_id  |  expected_salary

jobseekers table: it contains the below columns:

jobseeker_id  |  year_of_experience  |  name

My view file codes:

    <% @applicants_view.each do | applicants | %>

        <%=  applicants.jobseeker.name%>
        <%=applicants.jobseeker.year_of_experience %>
        <%= applicants.expected_salary %>

     <% end %>

My controller codes:
def applicantsList

   @applicants_view = AppliedJob.paginate(page: params[:page],

:per_page => 4).order('applied_jobs_id DESC')
end

My applied_job model codes:
class AppliedJob < ActiveRecord::Base

    belongs_to :jobseeker
end

My jobseeker model codes:
class Jobseeker < ActiveRecord::Base

    has_one :applied_job

end

When I try to show data it's showing the below error
 C:/wamp/www/hire_us/app/views/applicants_list/applicantsList.html.erb
   where line #51 raised:

  undefined method `name' for nil:NilClass

  Rails.root: C:/wamp/www/hire_us
  Application Trace | Framework Trace | Full Trace

  app/views/applicants_list/applicantsList.html.erb:51:in `block in
 _app_views_applicants_list_applicants_ist_html_erb___1439381291_141337560'

  app/views/applicants_list/applicantsList.html.erb:49:in
    '_app_views_applicants_list_applicants_ist_html_erb___1439381291_141337560'

Someone can help me, please to identify where the bug?

A: You can avoid the error by adding
<% @applicants_view.each do | applicants | %>
   <% next unless applicants.jobseeker %>
   ...
<% end %>

or
<% @applicants_view.each do | applicants | %>
   <% applicants.jobseeker.try(:name) %>
   ...
<% end %>

redpajama_set_name: RedPajamaStackExchange


"Voodoo festival-goers came from far and wide to see some of the best talent music has to offer.
Arcade Fire posted the above picture with the message: "Brooklyn Friday and Saturday! Dress up and come party with us!"


Alternate download mechanism for packages?
My ISP (which is also my local telco), in an atrocious display of ignorance, messes with http traffic. In an attempt to preserve its over-priced telephone business it does things like block connections to skype.com, etc. It also does stupid stuff like block http requests with certain keywords in them. Apparently, one of these keywords is "proxy". So, my problem is that whenever I try to install the squid package it times out trying to download monitor_proxy.sh.
Is there any alternative download mechanism for packages? Maybe https? or ftp?
I hope you are not in the US because what your ISP is doing is very illegal.
I don't know of any alternate downloads but you should start looking into a VPN service.
No, I'm obviously not in the U.S. Dunno if it's illegal or not there but that doesn't matter: No one who has even a little bit of knowledge of how the internet works would do anything this stupid.
Thanks for the suggestion about the VPN. I have a FreeBSD box sitting in the U.S. so I just ssh'ed in configured IP forwarding and NAT, installed OpenVPN, configured it, made it my default gateway, and YAY!! I can install squid (as a bonus all the weird stuff that used to happen like connections being abruptly reset, parts of web pages not appearing, etc. have disappeared). I should have done this a long time ago. Thanks!


Spectacular New Construction! Custom-Crafted by a Premier South Boston Developer! Tucked away in the sought-after east side of South Boston, form and function converge in this distinguished 8-unit new construction development! Elevator for easy City living! Check out the perfect floor plan offered by Unit 3, it is a 1 bed plus den, 1.5 bath, open concept home with private outdoor space and deeded compact car parking. Features include wide plank European oak flooring, inset cabinetry with integrated appliances, a large format gas fireplace with custom mill work, and a stunning chevron marble bathroom. No expense or detail has been spared in a remarkable display of transitional, timeless design. Fall 2019 delivery. Don't Miss out on this Amazing opportunity!


Sales at House of Fraser rose 2.7% for the 39 weeks to October 25, boosted by new stores in Belfast and High Wycombe.
For year to January 26 House of Fraser sales were £1.03 billion, the business' first full year of trading since its acquisition by Highland Group in November 2006.
Year on year, sales were down 1.1%. However margin was up by 10 basis points. EBITDA for the period was £68.1m, up 30% on the equivalent underlying performance in 2007.
Around £100m worth of debt has been cancelled or repaid. Net assets were £214m, and net debt was £257.6m.
House of Fraser said that sales performance in the 39 weeks to October 25 had also benefited from the opening of the relocated store in Bristol's Cabot Circus in September.
House of Fraser is opening a shop in Westfield London in White City today. Go to our dedicated Westfield page for videos and pictures and live reports from the centre as it opens.


If you're an iPhone 6/6s user who shoots lots of photos or videos, you know the frustration of running short on storage just when you're capturing something unique. If you depend on your device for entertainment and document access on the go, you've likely watched the battery drain when you're far from the nearest charging station.
SanDisk's innovative new iXpand Memory Case and iXpand Battery Pack address issues related to storage, power and privacy, all in a sleek, stylish package that protects your phone.
The low-profile iXpand Memory Case is available with 32GB, 64GB or 128GB of storage. That means no more deleting photos to free up a little file space. The modern multi-tasker has a hard plastic shell that's available in gray, blue, red or teal, and each comes equipped with a rubber lining. While this adds a little extra grip, it also protects your screen from scratches when the phone gets tossed in a bag or buried under a pile of documents.
For power users, the iXpand Battery Pack (sold separately) easily snaps in and out of the case whenever you need it. The charger automatically starts working when your phone battery runs low, so you'll never lose power in the middle of a project. It's also light and slim enough to be incredibly travel-friendly. Now, you can keep taking pictures, watching movies or playing music without interruption, whether you're at your desk or on the road.
In typical SanDisk style, the iXpand Memory Case comes with a convenient companion app that helps you easily manage all your content. The app automatically categorizes files so that your documents, photos, videos and music are organized and easy to access. Once you've uploaded everything through the app, you can browse photos, build custom playlists, and play videos and music directly from the case. You can set up automatic transfer and camera roll backup to save files as you go. Plus, if you plug in the optional battery pack, you can manage that extra power with just a few taps, as well.
Road warriors will be happy to know that security features come standard with the iXpand technology. Files can be password protected through the app, and the SanDisk SecureAccess software built into the case keeps info safe as you transfer between desktops, laptops and your iPhone. This provides some great peace of mind when you're working from airports, cafes or other places where you want to keep prying eyes from accessing your files.
Life gets busy. When you're juggling projects, trips and social events, you shouldn't have to stop to organize files, backup important documents or charge your phone. Thanks to SanDisk's iXpand Memory Case and iXpand Battery Pack, you no longer have to.
The iXpand Memory Case is available at Amazon.


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Artemisinin-based combination therapy (ACT) is the mainstay of global efforts for treatment of Plasmodium falciparum malaria, but decline in its efficacy is the most important obstacle towards malaria control and elimination. Therefore, the present molecular analysis provides information on putative mutations associated with artemisinin resistance in P. falciparum clinical population unexposed and exposed to artesunate 4 years after adoption of ACT as the first-line anti-malarial therapy in Iran.
In this study, blood samples (n = 226) were collected from uncomplicated P. falciparum-infected patients from different health centers of Chabahar district in Sistan and Baluchistan province in the south-eastern part of Iran, during 2003 to 2010. All collected isolates were analysed for putative candidate mutations (TTA) L263E (GAA), (GAA) E431K (AAA), (GCA) A623E (GAA) and (AGT) S769N (AAT) of pfatpase6 gene using nested PCR/RFLP, followed by sequencing. Furthermore, the gene copy number was assessed by real-time quantitative PCR (RT-qPCR) in the presence of SYBR green.
Neither the pfatpase6 L263E nor the A623E mutation was detected among all examined isolates. The E431K mutation was found in 23% of the analysed samples unexposed to ACT; however, it was detected in 17.8% (34/191) of P. falciparum isolates exposed to artesunate after 2007. High frequency of this single nucleotide polymorphisms (SNP) (overall 18.6%) among both examined groups (X2 test, P>0.05) indicated that this SNP should be considered as an unrelated mutation to artemisinin resistance. In contrast, S769N mutation was not detected in unexposed isolates; however, it was found in 2.6% (5/191), four years after introduction of ACT in this malaria setting. Also, detected SNPs were not significantly frequent in both unexposed and exposed examined isolates (X2 test, P> 0.05). Investigation in the copy number of pfatpase6 gene revealed a similar number of copy (n = 1) as in an isolate sensitive to artemisinin.
Taken together, the results suggest, in particular, that pfatpase6 S769N gene needs more consideration for its possible association with artesunate resistance among P. falciparum isolates.
Plasmodium falciparum resistance is one of the most important public health issues in many endemic countries in the recent years. In the absence of a fully-protective anti-malarial vaccine, malaria control heavily relies on the use of drugs for treatment or prophylaxis. However, the increasing failure of the predominantly used and safe drugs, especially chloroquine (CQ) and sulphadoxine-pyrimethamine (SP), has been a serious obstacle towards global malaria control. Consequently, to overcome the resistance problem, the World Health Organization (WHO) and health authorities in malaria endemic countries recommend the use of a therapeutic combination. They recommend the use of artemisinin derivatives in combination with other drugs (ACT) in order to provide a better efficacy and avoidance of resistance nationwide [1, 2]. As a result, following increased CQ and SP resistance, artemisinin and its derivatives gradually became the mainstay of falciparum malaria therapy. Besides, previous experiences with other anti-malarial drugs showed that resistance appears after long-term use. Recent reports of the reduced artemisinin sensitivity of P. falciparum parasites in known foci in Cambodia and Thailand, and new suspected foci in Myanmar and Vietnam may suggest a decline in ACT efficacy [3–5]. Therefore, due to the essential role of ACT in malaria control and elimination, it is crucial to have a regular surveillance of resistance to determine whether resistance to artemisinin has developed or not.
The molecular mechanisms involved in underlying resistance to artemisinin derivatives are not clearly understood and identification of molecular markers would provide insight into these mechanisms. Also, up to the present, artemisinin resistance has not been clinically documented, but it has been reported that the susceptibility of P. falciparum to artemisinin derivatives is declining in several parts of the world . Laboratory studies have shown that genetically stable and transmissible artemisinin-resistant rodent malaria parasite could be selected through prolonged exposure of drug-sensitive lines to low and increasing levels of artemisinin .
Molecular markers are recommended as the earliest technique to detect emerging drug resistance; therefore, the identification and monitoring of genes and mutations, which correlate with resistance to artemisinin and its derivatives, are essential for the evaluation and monitoring of ACT. Previous studies on P. falciparum have provided evidence that few genes have been associated with artemisinin resistance and these genes are sarcoplasmic/endoplasmic reticulum Ca2+-ATPase6 (pfatpase6) [8, 9], pfmdr1[10–13] and P. falciparum multidrug resistance protein 1 [14–18]. However, PfATP6 protein might be the major target of artemisinin in P. falciparum, as the drug completely inhibits the activity of this protein [8, 9]. Moreover, recent studies performed with both eukaryotic models and field isolates have proposed that single nucleotide polymorphisms (SNPs) in the pfatpase6 gene are involved in altered in vitro sensitivity to artemisinin or its derivatives [9, 19, 20]. Also, different studies have previously shown that pfatpase6 contains a number of SNPs [21–23] and four of them (L263E, E431K, A623E, and S769N) have been reported to be associated with a considerable increase in artemether IC50 . It has been also found that the pfatpase6 S769N mutation is strongly associated with raised artemether IC50 in P. falciparum isolates from French Guiana . Besides, it has been suggested that the mutation at L263E codon is involved in the inhibition of PfSERCA by artemisinin [9, 19] and allelic exchange study showed reduced susceptibility to artemisinin in parasites expressing the L263E allele . The combination of two additional SNPs, E431K and A623E, was identified in one clinical isolate from Senegal that showed a considerable increase in artemether IC50 . Moreover, other polymorphisms have been identified in pfatpase6 gene: I89T in Thailand , H243Y in Central Africa  and silent mutation T2694A in São Tomé and Principe . In 2008, Dahlström and co-workers , who studied P. falciparum isolates from East and West Africa, identified 33 SNPs; three of which were found in a frequency higher than 5% at codons E431K, N569K and A630S.
In October 2007, Iran has switched its first-line treatment policy for uncomplicated falciparum cases to the use of ACT. Nowadays, there is no other anti-malarial drug to replace the artemisinin derivatives; thus, an urgent global priority is needed to prevent potentially emergence and spread of artemisinin resistance. The main goal of the present study was to examine the mutation(s) in the atpase6 gene of P. falciparum isolates unexposed and exposed to ACT prior and four years after the adoption of ACT as the first-line anti-malarial drug in hypoendemic malaria settings. In fact, this study attempted to determine whether drug pressure imposed by continuous deployment of ACT causes a potential selection of mutation(s) in pfatpase6 gene or not, in particular, candidate mutations (TTA)L263E(GAA), (GAA)E431K(AAA), (GCA)A623E(GAA) and (AGT)S769N(AAT).
In this study, blood samples were collected from different health centers of Chabahar district in Sistan and Baluchistan province in the south-eastern part of Iran. In this area, malaria transmission is year-round with two peaks: the first peak occurs during May to August and the second peak mostly happens around October to November and the majority of the cases are infected with Plasmodium vivax. Blood samples (n = 226) were collected from uncomplicated P. falciparum-infected patients, aged from 2 to 70 years old, during 2003 to 2010. The inclusion criteria were the presence of fever for the preceding 48 h (axillary temperature ≥ 37.5°C), mono-infection with P. falciparum, no intake of anti-malarial drugs for the preceding four weeks and no signs of complications. One milliliter venous blood was collected from P. falciparum-infected patients prior to treatment based on the National Guideline for treating malaria (CQ from 2003 to 2005, CQ-SP from 2005 to 2007 and SP-artesunate from 2007 onward). All blood samples were collected in a tube containing EDTA, stored at 4°C, and then transported to the main laboratory in Tehran. Furthermore, a written informed consent was obtained from all patients (adults or parents/legal guardians of children) who were participated in the study and an ethics approval was obtained from the Ethical Review Committee of Research of Institut Pasteur, Iran.
Parasite DNA was extracted from 300 μL blood samples of either continuous culture of K1 laboratory strains or field isolates of P. falciparum using the commercially available DNA purification kit (Promega, Madison, WI, USA), and kept at −20°C until use. DNA of P. falciparum was detected by nested-PCR amplification of the small sub-unit ribosomal ribonucleic acid (18ssrRNA) genes as described previously . The DNA was resuspended in a Tris-EDTA buffer (10 mM Tris–HCl, pH 8.0, 0.1 mM EDTA) and kept at −20°C until use.
The fragment of pfatpase6 gene was amplified by nested PCR with oligonucleotide primers and the cycling condition has been demonstrated in Table 1. Briefly, amplification was carried out in a final volume of 25 μL, including 1 μL nest1 PCR product as template, 250 nM primers, 10 mM Tris–HCl (pH 8.3), 50 mM KCl, 2 mM MgCl2, 125 μM each of the four deoxynucleotide triphosphates and 0.4 U Taq polymerase (Invitrogen, Carlsbad, CA, USA). Secondary PCR products were digested by four enzymes: ApoI (Fermentas, Vilnius, Lithuania), MBoII (Fermentas, Vilnius, Lithuania), Cac81 (New England Biolab, Beverly, MA, USA) and DdeI (Fermentas, Vilnius, Lithuania) and were used to identify mutations at positions L263E, E431K, A623E, and S769N, respectively (Table 1). Secondary PCR products and digestion fragments were resolved by electrophoresis on a 1.5 and 1.5-3% agarose gel, respectively.
Based on PCR-RFLP results, 40 samples (n = 20, 2003–2006; n = 20, 2007–2010) were selected for sequencing analysis of the entire pfatpase6 gene. Therefore, 4032 bp (nt = −66 to 3966 in GenBank, accession no. AB121053) of pfatpase6 gene, including all reported artemisinin-resistant SNPs, was amplified by using the oligonucleotide primers and the cycling condition has been described in Table 2. Each pair of primers was designed to overlap 70 to 100 bp to cover all sequencing region. The amplified fragments were gel purified using the QIAquick Gel Extraction kit (Qiagen, Hilden, Germany) following the manufacturer's instructions. Direct sequencing of the DNA fragments was performed in both directions for each PCR product using the dideoxy chain termination procedure (Chemistry V3.1, Applied Biosystems) and also the 3730XL DNA analyzer (Applied Biosystems) by MilleGen sequencing service (Labege, France). Nucleotide and amino acid sequences were aligned and compared with wild-type sequences (accession no. AB121053) using CLUSTAL W.
The gene copy number was assessed by real-time quantitative PCR (RT-qPCR) using a StepOneTm (Applied BiosystemsTM) in the presence of SYBR green. Oligonucleotide primers (F: TTGCTGCTATACCAGAAGGATTGC and R:TGTTGTCATTTGATTTGTTGTAAGGG) were manually designed (accession no. AB121053) and verified by both Primer Express® Software for real-time PCR (Version 3.0, Applied BiosystemsTM) and Gene Runner (Version 3.05). Amplification was carried out in triplicate using the default thermocycler program in a total volume of 20 μl in a 48-well plate (Applied BiosystemsTM), containing 10 μl 1× SYBR®Green PCR Master Mix (Applied Biosystems, Warrington, UK), 0.75 μl of each of the sense and anti-sense primers (10 μM), 5 μL genomic DNA (20 ng) and 3.5 μL water, with amplicon size of 179 bp. The program was: pre-incubation at 95°C for 10 mins, followed by 40 cycles at 95°C for 15 seconds and at 60°C for 1 min. A PCR negative control with no template was used to check the presence of the dimmers and finally an electrophoresis was used once at the end of the first optimization plate to confirm that a single product of the target gene is present. In this study, the 2−ΔΔCtmethod for relative quantification was used to estimate the copy numbers of pfatpase6 gene. In this method, to estimate gene copy numbers in unknown samples, at least one calibrator consisting of template DNA with known copies of pfatpase6 (P. falciparum K1 with one copy of atpase6) and a house-keeping gene of constant copy number in all samples (pf-β-tubulin gene) are required. The formula for the estimation of gene copy numbers in clinical isolates of P. falciparum is as followed: ΔΔCt = (Ct target gene - Ctpf-β-tubulin)unknown sample - (Ct target gene - Ctpf- β-tubulin) K1. Then, the result for each sample was expressed in N-fold changes in unknown samples (2−ΔΔCt). A minimum of two experiments was carried out for each sample and the results were expressed as the N-fold copy number of a given gene relative to P. falciparum K1, by calculating the mean between the two experiments. In the case where N-fold was between 0.5 and 1.6 (0.5 < N-fold < 1.6), it was accepted that the test sample harbored a single copy of the target gene.
In the present study, β-tubulin as a house-keeping gene was amplified using the primers described previously ; F: TCGTCAACTTCCTTTGTGGA and R: TCCCATTCCCACGTTTACAT). To normalize quantitative data in each reaction, all samples were analyzed in triplicate within each LightCycler run and the average value was accepted if the standard deviation was lower than 0.24. The genomic DNA samples used were the same as those used for gene amplification and sequencing. The purity and quantity of DNA were determined by UV spectrophotometry. To determine RT-qPCR efficiencies, standards consisting of 2-fold serial dilutions of genomic DNA were freshly prepared from concentrated stocks for each experiment. Genomic DNAs in the range of 64 to 1 ng were used as quantification standards for the LightCycler calibration curve. Then, amplifications were performed on the same diluted samples using primers for the reference (pf β tubulin) and the target genes. The average Ct was calculated for both reference and target genes, the ΔCt (Ct target gene - Ctpf β tubulin) was determined and the log DNA dilution versus ΔCt was plotted. The efficiency of the PCR should be 90-100% (-3.6 > slope > -3.1). If the absolute value of the slope was close to zero (0.1), the efficiencies of the target and reference genes were similar, and thus the ΔΔCt calculation could be applied.
All statistical analyses were performed using SPSS statistical package (version 16.0). Frequencies of mutations and haplotypes among the groups were compared using the X 2 test. P<0.05 was considered statistically significant.
In this investigation, pfatpase6 gene was successfully amplified in all field samples (n = 226). Of 226 P. falciparum isolates, 35 samples were collected before the adoption of ACT as the first-line anti-malarial drug in Iran during 2003–2006 with no artemisinin pressure. Also, 49 of 226 samples were collected in 2007, when the artemisinin pressure was very low. Although SP-artesunate was selected to replace CQ-SP in the study areas in year 2007 all malaria settings in Iran undoubtedly used SP-artesunate as the first-line therapy in 2008. Other 142 of 226 samples were collected after the full adoption of SP-artesunate in Iran.
All 226 P. falciparum samples were successfully genotyped. Surveillance of pfatpase6 SNPs within positions L263E, E431K, A623E, and S769N indicated no (0/226), 18.6% (42/226), no (0/226), and 2.2% (5/226) prevalence of pfatpase6, respectively (Table 3). The majority of the patients (80%, 181/226) was found to carry wild type while single mutant haplotype (L263K431A623S769), as the second predominant allele, was detected in 16.4% (37/226) studied isolates (Table 3).
* Unexposed P. falciparum isolates to ACT.
** Exposed to ACT with high pressure.
*** Exposed to ACT introduction of ACT as first-line anti-malarial therapy for 4 years.
According to the year of sample collection, before 2007, the prevalent haplotypes were wild type (77%) and L263K431A623S769 (23%); however, the most frequent haplotype in 2007-2010 was wild type (80.6%) (Table 3). In addition, the number of haplotypes increased from 2 to 6 after ACT adoption in Iran (Table 3). Before and after the introduction of ACT, the prevalence of pfatpase6 E431K mutation in Sistan and Baluchistan province was 23% (8/35) and 17.8%, (34/191) respectively (Table 3). However, S769N mutation was not detected in any unexposed isolates, but found in 2.6% (5/191) of the exposed isolates (Table 3).
The pfatpase6 gene of 4032 bp in length was successfully sequenced from the PCR products of 4060 bp by using 7 overlapping paired primers. Both non-synonymous and synonymous mutations were detected at different frequencies in comparison with the reference sequence (accession no. AB121053) as shown in Table 4. Sequencing analysis of samples confirmed the RFLP results. In addition, nine different non-synonyumous mutations at codons 89 (87.5%), 230 (%2.5), 355 (15%), 431 (57.5%), 569 (87.5%), 630 (100%), 683 (97.5%), 769 (6.5%) and 870 (2.5%) were detected in high frequency among both unexposed and exposed P. falciparum isolates (X2 test, P>0.05) (Table 4).
In this study, to obtain optimal efficiency, RT-qPCRs were first optimized for each gene by both PCR reagent concentrations and primers and for each gene (including target, house-keeping and reference genes), PCR efficiencies were obtained for about 95% of all cases (data not shown). Regarding the further validity of the RT-qPCR assay, the copy number of pfatpase6 gene was compared with the laboratory clone P. falciparum K1 with only one copy number for the mentioned gene. After finding the optimal conditions for pfatpase6 gene, the estimated gene copy number of all examined field isolates was 1.0 equal to the reference isolate K1.
ACT is the mainstay of global efforts to control P. falciparum malaria, but suspected failure of artemisinin is the most important obstacle towards malaria control and elimination . At the present, no alternative classes of anti-malarial drugs are available to replace the artemisinin derivatives; hence, an urgent global priority is needed to prevent potentially the emergence and spread of artemisinin resistance. To achieve this goal, determining the molecular mechanism of artemisinin and its derivative resistance is very important. Therefore, the present molecular analysis provides, for the first time, baseline information on putative mutations (L263E, E431K, A623E and S769N) associated with artemisinin resistance in P. falciparum population unexposed and exposed to ACT in low endemic areas of Iran. In this study, by using nested PCR-RFLP followed by sequencing analysis, neither pfatpase6 L263E nor A623E mutations was detected among P. falciparum isolates unexposed (before 2007) and exposed (after 2007) to ACT. In addition, L263E mutation was never found in field isolates [3, 19, 20, 22, 23, 26, 29–33], similar to the present finding, and its association with artemisinin resistance needs more studies to be confirmed.
Concerning E431K, 23% of the isolates harbored this SNP in the collected samples before 2007; however, 17.8% were detected in P. falciparum samples exposed to artesunate after 2007. The pfatpase6 E431K mutation has been suggested to be associated with increased artesunate IC50 in Senegal  and this SNP has also been reported from African, Asian and South American P. falciparum isolates. In the present findings we found a high frequency of this SNP among P. falciparum population unexposed and exposed to artesunate, indicating that this SNP is unlikely associated with artemisinin resistance. Furthermore, the most prevalent haplotype was wild type with a frequency of 77% before 2007 and 80.6% after 2007. The second most frequent haplotype was L263K431A623S769 with 23% and 15.2% frequency before and after ACT adoption in Iran, respectively. In a previous in vitro study, this haplotype with single mutation was sensitive to dihydroartemisinin  and the results suggested that the high frequency of E431K among Cameroonian P. falciparum isolates might be a warning signal for artemisinin drug resistance. Also, although this mutation in high frequency (overall 18.6%) was found among Iranian P. falciparum isolates, the non-significant prevalence (X2 test, P>0.05) among parasite population unexposed and exposed to ACT suggested that this mutation might have not been selected under artemisinin pressure.
Pfatpase6 S769N mutation has been reported to affect the PfSERCA activity with increased artemether IC50 and it was originally found in P. falciparum field isolates from French Guiana . However, it was not reported from China , Tanzania , Niger  and Brazil  with a different level of malaria endemicity. In parallel, this mutation was not detected in 35 Iranian P. falciparum isolates collected during 2001-2002 when CQ was used as first-line anti-malarial therapy . In addition, in the present investigation, further analysis on P. falciparum isolates collected during 2003-2006 (when CQ-SP was used as the first-line treatment) revealed no S769N mutation. Since ACT was used as the first-line treatment for uncomplicated falciparum malaria in Iran (in 2007), the frequency of this mutation has started to increase (2.6%). Detection of this mutation in only parasite isolates exposed to artesunate might suggest the likely selection of this mutation by artemisinin pressure as the previous study suggested the pfatpase 6 S769N mutation as a potential molecular marker for P. falciparum resistance to artemether . Also, the most frequent haplotype before and after ACT adoption was wild type, but the number of haplotypes increased from 2 to 6 in parasite isolates unexposed and exposed to ACT, respectively (Notably, parasites carrying pfatpase6 S769N mutation). Nonetheless, recent report by Cui et al. , using allele exchange strategy revealed that 3D7 parasite carrying 769N mutation was still sensitive to artemisinin and its derivatives. This evaluation was done by in vitro response of transgenic lines to aforementioned drugs . Although this result argue against the predicted role of pfatpase 769N in resistance to artemisinin and its derivatives, but many variables including genetic backgrounds of the parasites may significantly influence on parasite resistance to various anti-malarial drugs [38–42]. Therefore, due to the conflicting data reported by different studies [19, 37], present study] the role of this candidate mutation in artemisinin and its derivative resistance needs further study.
In conclusion, the pfatpase6 genotype and its copy number might make early warning signals for the emergence of ACT resistance and provide baseline data for anti-malarial drug policy. Until now, there is no evidence for assuming that artemisinin resistance has occurred in Iran (Iranian Center for Disease Management and Control, surveillance report, unpublished). Therefore, the present results suggest that, to confirm and distinguish a mutation associated with artemisinin resistance, more studies are required among P. falciparum population unexposed and exposed to ACT from global malaria endemic setting. However, the pfatpase6 S769N mutation needs more awareness for its association with artesunate resistance and its value as a molecular marker for monitoring artemisinin resistance among P. falciparum population remains to be validated in areas where ACT has been used for a longer period. Introducing such a molecular tool could support the national and global malaria control and elimination programmes.
We acknowledge the co-operation of the Center for Diseases Management and Control (CDMC), particularly Dr. M.M. Gouya. We are also grateful to the Zahedan University of Medical Sciences and staff in the Public Health Department, Chabahar district, Sistan and Baluchistan province for their generous collaboration, for their logistic support in collecting blood samples, microscopy-based malaria diagnosis and reviewing malaria slides in the field. We are indebted to the patients and their families and their willingness to participate in this study. This study was supported by a grant from the Iranian Deputy for Research of the Ministry of Health, and Institut Pasteur, Iran.
SZ designed the study, developed the experimental protocols, supervised field and lab works, finalized the interpretation of the data and wrote down the manuscript. SH, SP and MA performed the lab work and also helped in analysing the data. ND and AR have also contributed to the analysis of the data and critically reading the content of the manuscript. All authors read and approved the final manuscript.


The AMERICAN FLAGLIGHTER provides the perfect solution to great flag illumination with virtually no unwanted uplight. The wider reflector/shade of the American Flaglighter provides a slightly less intense but wider light distribution from the same wattage lamp, providing a measure of security to a larger area around the base of the pole. Lamp sources: 175W/250W Metal Halide, 85W/165W Induction, or 54W/72W LED.


text:
Reference : Country studies for the project 'Protection against discrimination on grounds of sexu...
Focus Areas : Law / European Law
Title : Country studies for the project 'Protection against discrimination on grounds of sexual orientation, gender identity and sex characteristics in the EU, Comparative legal analysis, Update 2015
Author, co-author : Vysotskaya, Volha [University of Luxembourg > Faculty of Language and Literature, Humanities, Arts and Education (FLSHASE) > Integrative Research Unit: Social and Individual Development (INSIDE) >]
Publisher : The European Union Agency for Fundamental Rights (FRA)
Keywords : [en] equality ; discrimination ; gender identity
Abstract : [en] The Employment Directive was transposed into the "Equal Treatment Law" of 28 November 2006 on equal treatment (the "Law"). 1 The law's definitions of direct and indirect discrimination includes all the elements in the definitions of the concept given in the Employment Directive, in particular less favourable treatment because of an individual's sexual orientation as grounds for a finding of direct or indirect discrimination. The Equal treatment law amends the Labour and Criminal Codes, following very closely the provisions of the Employment Directive. By transposing both the Employment Directive and the Racial Equality Directive in the same piece of legislation, the Law broadens the Employment Directive's material scope of the application, the "Equal Treatment Law" . The law also provides for the establishment of a Centre for Equal Treatment (CET) (Centre pour l'égalité de traitement, CET), which began to set up the elements necessary for its operation at the end of 2007. The CET has emphasised that its mission provides it with no binding powers over institutions or private persons that do not wish to collaborate with it; and believes that its investigative powers should be strengthened.
Other URL : http://fra.europa.eu/sites/default/files/fra_uploads/country-study-lgbt-legal-update-2014-lu.pdf
Commentary : This document was commissioned under contract as background material for comparative analysis by the European Union Agency for Fundamental Rights (FRA) for the project 'Protection against discrimination on grounds of sexual orientation, gender identity and sex characteristics in the EU, Comparative legal analysis, Update 2015'. The information and views contained in the document do not necessarily reflect the views or the official position of the FRA. The document is made publicly available for transparency and information purposes only and does not constitute legal advice or legal opinion
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There are some naysayers who think climate change doesn't exist. It's a greenhouse, or should I say, outhouse myth. Don't get me wrong. I am not one of them. To me, the evidence is unequivocal. Sea levels are rising and the world is getting warmer. Time is also running out. Some scientists believe we are close to midnight in terms of doing anything meaningful to stop climate change from destroying our world.
But this is not meant to be a discussion on climate change as worthy as that might be. What I am really wittering on about here is the remarkable something that just happened in China, indirectly related to climate change. It may change the way we live. It will certainly change the way we build.
A Chinese company has just constructed an apartment block, and what is quaintly described as a McMansion, using (wait for it) a 3D printer. Go figure. In case you are wondering, is it the same kind of ink jet print used to print copy on pieces of A4 paper? Absolutely. Obviously, we are talking about a ginormous version. The printer is 105 feet or 30 metres long, 33 feet or 10 metres wide and 21 feet or 6 metres high. In fact it resembles a giant piping bag used to decorate cakes. But instead of using ink, the giant 3D printer lays down a cement like material partly made from construction waste, like concrete dust, fiberglass strands, sand and a hardening agent to build walls and ceilings tough enough to withstand an earthquake. The company called Winsun Decoration Design Engineering, constructed a six storey apartment and a mansion, of about 11 thousand square feet or 1,100 square metres, side-by-side in an industrial park in eastern China. And how long did it take for them to do it? This part you are not going to believe. The answer is less than a week, for both buildings. The five storey apartment took one day to print and five additional days to assemble. Not only is it quick, it is environmentally friendly.
The construction method is extraordinary. 3D printing works through a process of layering. The printer reads a file, the same as a deskjet reads an image, and then translates that into a physical object the way your printer spits out ink on to a page—one strip at a time. The "ink" in this 3D printer is recyclable construction material that shoots out of a nozzle and onto a platform. It will gradually build layers with curves until it reaches the top. You could call it a bottom-up process. What is so exciting about this technology is that it's better suited to creating or prototyping new shapes rather than reproducing existing ones. The Chinese company Winsun constructed preforms for its two buildings that are simply bolted together in much the same way as you would do if you were building a giant Leggo set. The prefabs are then reinforced with steel and filled with insulation, which is the procedure followed in every other traditional construction project. Of course this method of building has produced some remarkable statistics. The company says it cut construction costs, when compared to a standard built project, by 60 percent with 70 percent lower production time and an 80 percent lower cost. The mansion, although huge in size, cost as little as US$161,000 to build. Speed is the key feature. The company says completing the various elements of the structures takes only 30 percent of the time needed to finish a similar structure using traditional construction methods. The company wants to use 3D printers to build bridges and skyscrapers.
This is definitely a new way of building and the possibilities are endless. In fact, last year, Winsun wanted to prove a point so it constructed ten houses in less than 24 hours, each of them about 2,100 square feet, costing US$5,000 to build. Can you imagine how this would benefit low-income housing projects, disaster relief in poor countries, or refugees fleeing war? Instead of living in tents or other crude or rudimentary forms of shelter, these houses could be either temporary or permanent accommodation. These developments have caused a lot of people all over the world to suddenly get very excited. It is not hard to see why. There are key benefits, too good to pass up, for using a 3D printer as a method of construction. Ironically, as a design becomes more complex, the cost of 3D printing drops substantially when compared to traditional methods. And the reason for that is because a 3D printer can literally reinvent the wheel. It can create any prototype you want. The only limit is your imagination. Or course that has architects, all over the world, positively licking their lips. It means that architects can dream up and build all sorts of fantastic structures that are currently either too difficult or too expensive to construct using traditional methods. These kinds of buildings would also be safer for the people living in them as well as the people constructing them. Structures built with curves are inherently stronger. Pillars with greater density towards their edges are sturdier and because fewer construction workers will be needed to build these buildings there will be fewer deaths and injuries. But nothing is perfect. It also means there will be less jobs in the construction industry.
3D printers can also be used for construction of a different kind. For years, scientists were able to "print" types of human tissue using a 3D printer, but in a significant leap forward by both American and Australian researchers, scientists can now make that human tissue survive on its own.
I think we all hope that you are Doctor Bertassoni.