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Agra Hadig or Atam Hatik is a ceremony in Armenian culture commemorating the first tooth coming in of a newborn baby. In the ceremony, the young baby is set before several symbolic items relating to different professional vocations. Whichever item the child first selects is meant to indicate what profession the child will likely gravitate towards as they grow up. Traditionally, the mother next sprinkles wheat over the child to wish for fruitfulness. The combination is meant to represent the spiritual connection between Agra (teeth) and Hadig (wheat).

The tradition is said to go back several hundred years but is most recently documented in the 19th century, originally as a divination to predict the gender of the child's next sibling. Under Soviet rule and the eventual Armenian diaspora the tradition changed to predicting the profession of the child in adult life.

Objects include: book for scholar, money for finance, jewelry for jeweler, spool of thread for tailor.

Tradition is to cook/boil wheat grains, which symbolize the "teeth". A thin veil is held over the child's head, and the grains are sprinkled over them, on top of the veil for good luck.

Then, the cooked wheat is eaten by the party attendees, with an assortment of toppings they can put on their bowl of wheat such as sugar, cinnamon, pomegranate seeds. Modern versions also include sprinkles, chocolate chips, mini M&Ms and the like.

See also
Zhuazhou
Doljanchi

References

Rites of passage

redpajama_set_name: RedPajamaWikipedia


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Sashin's notes
"I can readily see that 'The Selfish Gene' on its own, without the large footnote of the book itself, might give an inadequate impression of its contents."
Richard has lamented that he should have taken the advice of his friend Tom Maschler and named this book The Immortal Gene instead.
There is a misconception that many seem to have foolishly made about this book. Nothing about it states that we as organisms are necessarily selfish, or require selfish motives to function in the world. The title refers to the genes themselves.
Prior to the popularisation of the book, there was discourse in the scientific community about whether evolution by natural section took place at the level of the individual organism or at the level of the species.
That is; is it the fittest individuals that flourish and pass on their traits to the next generation or the fittest species. Altruistic behaviours in individual organisms including humans for the benefit of kin seemed to point to nature selecting for the fittest groups rather than individuals.
The Selfish Gene argues against this pointing to the gene as the unit of biology and therefore selection. It is because kin have genes in common that such behaviours can be found.
"Would I lay my life for a brother? No, but I would for two brothers or eight cousins."
J.B.S Haldane
From this point of view neither the organism nor the group are of ultimate significance. Rather, they are a part of the environment. The organism is a means of protecting and propagating genes from generation to generation.
To spell out the analogy in the title: genes construct organisms around them for their benefit. It is the genes themselves that are described as selfish, not the organisms they create.
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Saudi suspect in U.S. Navy base attack linked to anti-U.S. Twitter screed
By Brad Brooks
PENSACOLA, Fla
PTownsend
Dec. 8, 2019	09:03 am JST
Why would a logistics officer or someone mowing the grass or someone going to an aircraft operations class need a gun?
For most who've been in the military, with the possible exception of those shilling for the guns and ammo corporations, and those terminally afraid of people around them, especially those NOT from their demographic, that's a good question.
But it's a question for me that's easily answered: they DON'T.
theFu
PTownsend, like translation was involved.
Six other Saudi nationals were being questioned by investigators in Florida, three of whom were seen filming the incident, the New York Times reported, citing an unidentified person briefed on the investigation.
? How did the shooter get a handgun?
The other 3 need to be arrested. Conspiracy and accessory. We'll see how good the full support from the Saudi Govt really is when they arrest 25 others in their military back home.
There are many reasons that normal military people don't carry firearms on base. Why would a logistics officer or someone mowing the grass or someone going to an aircraft operations class need a gun?
He said his nephew "has his religion, his prayer, his honesty and commitments."
Euphemistic language to justify the slaughter of those with different beliefs.


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person JHED Login
Neurology and Neurosurgery
Otolaryngology–Head and Neck Surgery
Pediatric Diabetes and Endocrinology
Pediatric Gastroenterology and GI Surgery
Pediatric Heart
Pediatric Neurology & Neurosurgery
Pediatric Orthopaedics
Clinical Alliance
High-Value Health Care
Clinical Research Advancements
Precision Medicine Excellence
Endoscopic Ultrasound Measures Pressure in Hepatic Portal Vein
Gaining access to the liver through the stomach, Olaya Brewer Gutierrez and her therapeutic endoscopy colleagues at Johns Hopkins use endoscopic ultrasound to obtain a direct reading of the pressure in the hepatic portal vein.
Hypertension in the large vein that brings blood from the intestines to the liver is the main driver of cirrhosis. But when hepatologists need to know if the pressure in a patient's hepatic portal vein is too high, they've mainly had to rely on indirect measurement methods.
Physicians eschew direct measurement of portal pressure due to its invasiveness. Instead, they determine the pressure of two nearby veins and use the gradient to estimate portal pressure. Typically done by threading a guide wire through a vein in the neck, this method is also fairly invasive.
"We can do many different things in the same session. We don't have to bring the patient back or sedate them again and again." - Olaya Brewer Gutierrez
Olaya Brewer Gutierrez says it's time for some direct answers.
The Johns Hopkins gastroenterologist and endoscopist recently performed Maryland's first endoscopic ultrasound (EUS) measurement of a patient's portosystemic pressure gradient. Brewer Gutierrez hopes it's the first of many.
"When liver doctors need to know whether a patient is at risk for cirrhosis or cancer," she says, "we can now use EUS to see for ourselves."
The Johns Hopkins therapeutic endoscopy team uses a new, tiny manometer connected to a small needle that provides a real-time, direct reading of the hepatic portal vein pressure — the most effective way to determine a patient's risk for a host of dangerous liver diseases.
Brewer Gutierrez says EUS allows her to access the liver by way of the stomach. Through an endoscope, she inserts a 25-gauge needle into the patient's middle hepatic vein. The needle is attached to a thin tube, the other end of which is attached to the compact manometer. She pushes a few drops of a heparinized saline flush into the vein and waits for the meter to settle on a pressure reading. Without removing the needle, she calculates the average of three readings for a final pressure number. The process is then repeated in the umbilical portion of the portal vein and, finally, a gradient is obtained.
Brewer Gutierrez wants liver doctors in the Mid-Atlantic region to know that, in addition to the direct readings of portosystemic pressure, she and her therapeutic endoscopy colleagues offer other diagnostic services without the patient having to visit more than once.
"We can do many different things in the same session," she says. "We don't have to bring the patient back or sedate them again and again."
Brewer Gutierrez gives examples of what they can tell hepatologists about their patients.
"We can do an upper endoscopy, to see if the patient has esophageal varices," she says, referring to enlarged blood vessels in the organ that leads from the mouth to the stomach. The condition is a symptom of liver disease and can result in dangerous bleeding.
During the endoscopic session, says Brewer Gutierrez, she can switch to endoscopic ultrasound "and assess the liver, right in front of you. Now we can see the shape of the organ, the characteristics of the parenchyma. We can see if there are nodules, which would suggest cirrhosis. We can see the edges of the liver and look for changes that indicate cirrhosis or lesions. We can compress the organ with the scope to determine the stiffness. We can even perform a liver biopsy."
Brewer Gutierrez says Maryland's first EUS measurement of hepatic portal vein pressure spared a patient from having a double transplant.
"The patient was in end-stage kidney disease," she says, "and also had been diagnosed somewhere else with cirrhosis."
In the work-up before a kidney and liver transplant, Brewer Gutierrez used EUS to find that the patient's portal pressure was normal.
"It was less than 5 millimeters of mercury," she says, explaining that a manometer reading between 5 and 10 millimeters indicates subclinical portal hypertension.
While the patient was sedated in the endoscopy suite, Brewer Gutierrez looked for other signs of liver disease.
"We didn't find any lesions or nodules," she says. "No esophageal varices. Nothing. We were happy to tell the transplant team that the patient only needed a kidney transplant. The liver was fine."
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text:
Q: Simple thread pool in C++ Could anyone point me to a sample implementation of a thread pool in C++, please?  I'm looking for a very basic one without too much complexity, which would be suitable for a beginner in threading to study.

A: Look at Intel's Thread Building Blocks.  I don't know how well that library meets your "simple" criteria, but it seems to be very well thought-out and thorough.  I would think that it would be worth the effort to learn if you want to do threading in C++.
Boost also has some threading facilities.

A: It may be worth to check out Qt's QThreadPool implementation.

A: This is a very good one : threadpool its built uppon boost so its cross platform and everything , very easy to use as well

A: in his book Modern C++ Programming with Test-Driven Development, Jeff Langr walks thru a threadpool implementation. It's a busy wait implementation. I've modified it further here https://github.com/spakai/threadpool_future to support things such as signal-wait and futures.

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Vincitrice di 2 titoli WTA in singolare e 4 nel doppio, vanta la semifinale a Wimbledon 2022 come miglior risultato raggiunto in uno slam.

Carriera
Ha vinto in carriera trentadue tornei ITF, diciassette nel singolare e quindici nel doppio.

L'8 aprile 2013 ha sposato il suo allenatore, nonché ex tennista professionista, Charles-Édouard Maria. Nel dicembre dello stesso anno ha partorito una bimba, Charlotte, e dopo alcuni mesi è rientrata nel circuito.

In carriera, Maria ha conseguito due titoli WTA in singolare. Il primo sull'erba di Maiorca nel 2018, battendo Anett Kontaveit (3-6 6-4 6-3), Carina Witthöft (7-6(1) 6-3), Lucie Šafářová (7-6(3) 4-6 6-4), Sofia Kenin (6-2 2-6 6-4) e, in finale, la detentrice del titolo Anastasija Sevastova (6-4 7-5). Quasi quatto anni dopo, Tatjana vince il secondo titolo WTA della carriera sulla terra di Bogotà; partendo dalle qualificazioni, elimina Conny Perrin (6-1 6-0), Bárbara Gatica (2-6 6-3 7-6(3)), María Lourdes Carlé (6-1 6-2), Rebecca Peterson (6-1 6-4), Mirjam Björklund (6-1 6-4), Kamilla Rachimova (6-2 6-2) e, in finale, Laura Pigossi, con lo score di 6-3 4-6 6-2.

Negli slam, Maria ha ottenuto il miglior risultato in carriera al Torneo di Wimbledon 2022, dove è riuscita a raggiungere la semifinale grazie alle vittorie su Sharma (4-6 6-3 6-4), Cîrstea (3-6 6-1 7-5), la top-10 Sakkarī (6-3 7-5), l'ex campionessa del Roland Garros Ostapenko (5-7 7-5 7-5) e la connazionale Niemeier (4-6 6-2 7-5).

In doppio, Tatjana ha ottenuto 4 titoli sul circuito maggiore: il primo nel 2012 a Québeq City assieme a Kiki Mladenovic, battendo in finale Rosolska/Watson al super-tiebreak. Il secondo, Maria lo ha centrato 4 anni dopo a Bogotà con Lara Arruabarrena, superando Cé/Gámiz nell'ultimo atto. Il terzo è arrivato nel 2018 ad Acapulco assieme a Heather Watson, prevalendo su Christian/Santamaria in finale. Il quarto, Tatjana lo ha conquistato nel 2019 a Seul con Arruabarrena, battendo Carter/Stefani al super-tiebreak nell'ultimo atto.

Statistiche WTA

Singolare

Vittorie (2)

Doppio

Vittorie (4)

Sconfitte (5)

Statistiche ITF

Singolare

Vittorie (17)

Sconfitte (13)

Doppio

Vittorie (15)

Sconfitte (10)

Risultati in progressione

Singolare nei tornei del Grande Slam

Doppio nei tornei del Grande Slam

Doppio misto nei tornei del Grande Slam
Nessuna partecipazione

Vittorie contro giocatrici Top 10

Altri progetti

Collegamenti esterni


Transmission & Transfer Case : Eurotruck Importers, Your source for Mercedes-Benz off-road vehicles and spare parts!
Transmission filter o-ring. Must be installed with part number A 720 277 0095, Filter.
Plastic twist lock bell housing plug used on the 460 series G-Class. Original Mercedes-Benz part.
Clutch line used on the following W460 models: 230G, 230GE, 240GD and 300GD.
Cooling line for both the left and right side on all W460's with an automatic transmission. Made in the USA by CRP!
Nut used to secure the front output flange on the VG 080 transfer case. Original Mercedes-Benz part.
717 Getrag transmission output shaft seal made by Corteco in Germany.
Transmission input shaft seal ring for the GL 275 717.420 Getrag 5-Speed used in the W460 G-Wagen. Original Mercedes-Benz part.
Shifter boot for manual transmissions up to chassis number 030046. Original Mercedes-Benz part.
Non-flush mounted shifter boot for the W460 Mercedes-Benz G-Class trucks using the 4-speed transmission. Made in Germany by FeBi.
Large shifter bushing for the manual transmission shift linkage on the W460 series G-Wagen. Made by FEBI Bilstein in Germany.
Small shifter bushing for the manual transmission shift linkage on the W460 series G-Wagen. Made by FEBI Bilstein in Germany.
Shifter bushing clip for the manual transmission shift linkage on the W460 series G-Wagen. Six (6) clips are required. Original Mercedes-Benz part.
4-speed shifter handle used in the W460 G-Wagen. Original Merceded-Benz part.
Speedometer cable used on all W460 G-Wagens. Original Mercedes-Benz part.
Speedometer drive to VG80 transfer case gasket used on all W460 Mercedes-Benz G-Class trucks. Original Mercedes-Benz part.


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All Content Copyright emyvale.net
Master Frank McKenna Tydavnet Historical Society has been very active in recent times in researching various aspects of life in the Tydavnet and wider community and recording it for posterity. They also organise regular History Nights, where someone delivers a talk on a topic, incident or theme and this is discussed and recorded either in the written or an oral medium. They have discovered numerous stories and happenings that would never have been remembered or passed on to the next generation and so would have been lost forever. Some time ago, local man, Patsy Brady came across, more by accident than design, the grave of a Bernard McKenna and a Master Frank McKenna. There was a headstone and two other plaques, one of which was badly weathered. However there was enough information visible to spark a light of curiosity in the historical greedy mind of the viewer and so began Patsy's research and disclosure of the life and death of a Master Frank McKenna. Patsy began talking to senior citizens and people he knew, who might have information about the said Master Frank McKenna. Bit by bit the story unfolded and became more and more interesting. His connection with Tydavnet; his link with Monaghan town; his friendship with Lory Meagher, the great Kilkenny Hurler; his work and contribution to the life of Ballycran and Kircubbin Primary School. Pieces of the jigsaw were supplied by Seamus Sherry, who had been told the story by the late Lena Rooney, Michael McKenna, Eugene McCague and others but then Laurence McKenna, who has a daughter, Joanne, married in Ballycran and her three children are involved in the GAA there, gave Patsy the link to Ballycran and the story came together. Tydavnet Historical Society heard about the research and was keen to have the story told in public and when Cormac Sherry, Chairman of the Scotstown Club, heard of the GAA connection he encouraged Patsy to put a talk together and have it told in the GAA Complex in Scotstown. The event took place in the Complex on Wednesday, October 16th and a big crowd turned out for what was a very interesting and enjoyable night, hosted by the Historical Society and the GAA Club. Members of the Ballycran GFC, together with Joanne Clarke, nee McKenna, and her daughter, Anna, attended and were made very welcome. The event was opened by Heather Stirrat, Chairperson of the Historical Society, who recalled the recent deaths of two great community people, Mary McMahon and Packie Caulfield, both of whom had great stories and past memories and were regular contributors to the Historical Society. She then introduced Patsy who opened his talk by explaining how he first became interested in Frank McKenna and how he gathered the various parts of the story. He gave us the background to Frank McKenna whose parents Bernard and Mary Jane, nee Conlon, came from the Mullaghmore and Shee area but they purchased The Round House bar in Church Square, Monaghan, where Frank was born and educated at the Brothers. However his father, Bernard, who became Chairman of the Monaghan Urban Council, died at the young age of 53 and was buried at Urbleshanny. His wife remarried and moved to Belfast with the family. Frank went on to become a teacher and became Principal of Kircubbin Primary School. He was a great teacher, a fluent Irish speaker, excellent musician on piano and violin and taught singing. He also set up the Ballycran Hurling Club in 1939, and they were soon winning trophies and since then have become a powerful force in Ulster hurling, becoming once again the Down champions in 2019. Unfortunately Master Frank McKenna, as he was called, died in 1953, just like his father at the young age of 53, and he was brought back for burial in the family plot in Urbleshanny. Patsy quoted from various sources, highlighting the tremendous respect with which Frank was held in Ballycran and Tydavnet and Monaghan areas. A huge crowd met his cortege at the Border and accompanied it to the burial. His obituary, as printed in the Northern Standard, was a list of 'Who's Who' in the business and social life of North Monaghan and beyond at the time. Patsy also read extracts from a book produced by the Ballycran Club to celebrate their 50th anniversary in 1989 extolling Frank's great qualities and eulogising Master Frank McKenna. On Wednesday night Shane Gilmore from Ballycran told the gathering of the respect that the people of Ballycran have for Frank. He was a man, big in stature and big in mind, who was loved by young and old. He is still very much remembered in the area with the McKenna name everywhere. The McKenna pitch, the McKenna Centre, the McKenna Road, are all named after him and he is still held in the highest regard for what he contributed to the Ballycran area. This is only a brief synopsis of the details given by Patsy Brady and he was thanked by all for his extremely interesting presentation. There followed a Question and Answer session at which more relatives in the area were identified by Patsy and he assured all that his research will be handed over to the Historical Society for the archive. Refreshments were then served during which the chat continued but in all of it two questions remained unanswered – How did a Monaghan man set up and train a Hurling Team to become Down Champions and how did he become a close friend of the great Lory Meagher?
Truagh
Glaslough


As I mentioned in my last post I was scheduled to have my proposal hearing on May 14th. However, my first reader was suddenly asked to take another professor's place leading a TEDS class to Israel. So my proposal hearing has been rescheduled to May 6. Step three in the PhD program is coming up fast!


Northern Beaches Cycling Club Inc. is based in the northern suburbs of Perth, Western Australia. NBCC embraces the diversity of cycling interests of its members, whether its social rides, racing, mountain biking, or even cyclocross.
An ECU animator teamed up with Northern Beaches Cycling Club to create a video for the City of Wanneroo's bike park proposal.
The City's proposal for the future Alkimos regional centre, endorsed by the council in October, inspired Jake Broadby to create a 3D animation of the cycling facility.


text:
Q: Android - How to get Inapp purchase order details? On the server side, I need to verify an In-app purchase and get details of the order. For the first part, I can verify an In-app purchase via https://developers.google.com/android-publisher/api-ref/purchases/products/get this API call and get an order-id.
However, I could not find a way to get order details which include total invoiced amount, tax amount and the estimated revenue. I can see these details from Google Play Developer Console. I need to make a dashboard in which these pieces of information will be reported to our suppliers. Therefore I cannot use  Developer Console and need an API endpoint to get these data.
Is there such an endpoint to get order details of an in-app purchase?

A: There are APIs to download reports from Google Cloud Storage. The help page is here.


In 2019 a workshop, which also includes a tasting, will be presented to senior cellar assistants in Wellington, Breederiver, Vredendal and Upington. Qualified winemakers will present the workshops in these areas.
The Business of Wine & Food Tourism Conference, now in its fourth year, is set to take place at the Spier wine estate in Stellenbosch on 18 September. Its impressive panel of local and international speakers will centre their respective topics on the theme of 'Trends in Tourism'.
For the second time in three years, the TOPS at SPAR Wine Show will be staged in the nations capital.
The TOPS at SPAR Wine Show is set to premiere our first ever show in East London. Residents of this beautiful and historic city are eager to learn about wine and experience all that the Cape Winelands have to offer.


Our client's success is our success, and we take that responsibility very seriously. Read about some of our client's success stories through using RepCheckup.
Dr. Lombardi is a successful dermatologist in Tampa, Florida. As a concierge dermatologist, his reputation is key to gaining new patients. Through the use of RepCheckup, Dr. Lombardi was able to generate over 50 patient reviews while earning a 5-star rating.
Dr. Ava Shamban is a world-famous dermatologist who has found additional success managing her brand's online reputation through the RepCheckup tool to ensure that she receives feedback from patients to continue improving her practice.
Dr. Vigdorchik is a highly qualified surgeon in New York City. Despite this, he has struggled with fake negative reviews being used to smear his reputation. Through the use of RepCheckup, Dr. Vigdorchik has successfully managed his practice's online reputation.
Dr. Romano is a successful orthopedic surgeon in the Chicago area. Passionately serving his community's needs, Dr. Romano used RepCheckup's management tool to make his online reality reflect the quality of his practice.
A local Buffalo company, National Overhead Door has been able to keep in close contact with clients and gather reviews to manage their reputation with ease by using RepCheckup.
A.G Roehrig has been in business for over 60 years, serving homeowners of Western New York with high quality HVAC and Waste Oil Systems. Through the use of RepCheckup, A.G Roehrig has been able to boost their online reputation to accurately reflect the standards of their business.


text:
Browsing Alfredo Spagnu
ITALY30 March 2012, Avellino, Italy - The players of Avellino and Benevento on the field for the 29th round of the First Division in the Pro League.
ITALY30 March 2012, Avellino, Italy - Crowd scene of the Avellino and Benevento match as players come onto the field for the 29th round of the First Division in the Pro League.
ITALY30 March 2012, Avellino, Italy - Players of Avellino and Benevento teams on the field for the 29th round of the First Division in the Pro League.
ITALY30 March 2012, Avellino, Italy - The players of Avellino and Benevento coming onto the field for the 29th round of the First Division in the Pro League.


With the island breeze grazing their cheeks, Kelly made her way down the beach to her waiting groom. Looking dapper in a linen suit, Ed was the picture of calm as he focused on his stunning bride. With family following along in excitement, they were treated to a heartfelt foray into a Maui Wedding ceremony. After promising forever and becoming husband and wife, our gorgeous newlyweds wanted a wide variety of back drops for their romantic couple's portraits. That's exactly what they got as Ironwoods Beach did not disappoint. Between the golden sand, interesting walkway, and dramatic cliffs, this a photo collection not easily forgotten. Our beaming and beautiful couple as an excellent subject doesn't hurt either! ;o) We want to thank Kelly and Ed for letting the Simple Maui Wedding team share in the joy of their special day. Best Wishes & Warm Aloha!!!
Check out the Maui Wedding of Joshua & Boram!


A three acre lot with 400 feet on the Rangeley River with access to Rangeley Lake! This is a nicely wooded parcel, offering privacy and numerous wildlife sightings. Located in the village of Oquossoc, this lot is walking distance to shops, restaurants and marina. And snowmobile access would be right out your front door.


text:
How Quaaludes got into Cosby's courtroom
The story behind Cosby's long, failed fight to keep an explosive deposition out of court.
Jessica M. Goldstein Twitter Apr 17, 2018, 2:10 pm
NORRISTOWN, PA - APRIL 16: Bill Cosby leaving the Montgomery County Courthouse for the sixth day of his retrial for sexual assault charges on April 16, 2018 in Norristown, Pennsylvania. CREDIT: Gilbert Carrasquillo/Getty Images
Bill Cosby swears all he gave Andrea Constand was Benadryl.
An incendiary exchange from a 2005 deposition does not contradict that point. But it does offer the closest thing to a confession from Cosby that the prosecution is likely to get: Cosby's admission that he obtained Quaaludes with the intent of giving them to young women with whom he wanted to have sex.
Cosby's criminal retrial, expected to last at least a month, is heading into its second week. Constand alleges that Cosby drugged and sexually assaulted her. Cosby maintains that their encounter was consensual. He has been charged with three counts of aggravated indecent assault, each of which carries a maximum sentence of ten years.
On Tuesday morning, Judge Steven T. O'Neill ruled that prosecutors could introduce evidence about Cosby's prior statements about Quaaludes, "though he cautions that he may still block some references in the moment," the Philadelphia Inquirer reports.
EXCERPT FROM 2005 DEPOSITION OF BILL COSBY. CREDIT: ASSOCIATED PRESS
The ruling is a blow for the defense and for Cosby personally, who has spent over a decade trying — and, lately, failing — to keep the deposition hidden from the public.
'…you were going to use these Quaaludes for young women that you wanted to have sex with?'
Back in 2005, then-district attorney Bruce Castor declined to bring charges against Cosby, citing both a lack of evidence as well as Constand's delay in reporting (she went to the police one year after the alleged assault), so Constand filed a civil suit against Cosby instead.
Dolores Troiani, Constand's attorney at the time, grilled Cosby for four days in a Philadelphia hotel. During those days of questioning, Cosby confessed to a multitude of ethically questionable actions. As the New York Times, which later obtained the full transcript, reported:
He was not above seducing a young model by showing interest in her father's cancer. He promised other women his mentorship and career advice before pushing them for sex acts. And he tried to use financial sleight of hand to keep his wife from finding out about his serial philandering.
When detectives asked — not for the first time — whether Cosby had ever had sex with Constand, Cosby answered, "Never asleep or awake."
NORRISTOWN, PA - APRIL 16: Andrea Constand, plaintiff for the Bill Cosby trial, arrives at the Montgomery County Courthouse for the sixth day of his sexual assault retrial on April 16, 2018 in Norristown, Pennsylvania. CREDIT: Dominick Reuter-Pool/Getty Images
Cosby and Constand settled out of court for $3.38 million, an amount that both parties kept secret, per a confidentiality clause, until the defense revealed it at the retrial. And excerpts from this deposition, with all its potentially damning revelations, remained sealed for ten years.
Does a 'public moralist' have a right to privacy?
On July 6, 2015, Judge Eduardo Robreno, who'd presided over Constand's case since it began, released excerpts from the 2005 deposition along with other court papers from the case. Their ultimate publication is a credit to the hustle of Associated Press reporter Maryclaire Dale, who spent ten years unsuccessfully petitioning Robreno to unseal them and who, as dozens of new Cosby accusers came forward in late 2014 and early 2015, petitioned Robreno again.
Bill Cosby is on trial again. America has changed.
A sexual assault allegation from 2004 collides with the 'Me Too' movement in 2018.
Cosby's attorneys battled to keep the deposition sealed, citing Cosby's right to privacy. To publish the documents, they argued, would divulge to the masses "intimate knowledge about his sex life," embarrass Cosby, and violate the confidentiality agreement both Cosby and Constand signed.
But Robreno contended that by asserting his status as a role model — one who frequently spoke about what did and did not constitute acceptable behavior — Cosby essentially relinquished that right.
Roberno specifically pointed to one infamous address, which came to be known as the "Pound Cake Speech," that Cosby delivered in May 2004. Speaking at an NAACP event commemorating the 50th anniversary of Brown vs. Board of Education. Cosby berated black Americans for blaming racism for their problems, giving their children "names like Shaniqua, Shaligua, Mohammed and all that crap," and dressing "with their hat on backwards, pants down around the crack."
Speeches in this vein, Judge Robreno decided, prove that Cosby purported to be a "public moralist" and, in doing so, "voluntarily narrowed the zone of privacy that he is entitled to claim." As Robreno wrote in his ruling:
"The stark contrast between Bill Cosby, the public moralist and Bill Cosby, the subject of serious allegations concerning improper (and perhaps criminal) conduct, is a matter as to which the AP — and by extension the public — has a significant interest."
"Significant interest" turned out to be an understatement.
Reopening the case against Cosby
Of all the striking answers Cosby gave in the deposition, one single-word response stood out from the rest as if typed in neon ink: When asked if he intended to give Quaaludes to young women he planned to have sex with, Cosby said "yes."
By the time this deposition was published, over 40 women had publicly accused Cosby of sexual misconduct. Many of those women say that, before he assaulted them, Cosby drugged them, either by offering them pills or slipping drugs into their drinks.
The reckoning of Bill Cosby: a comprehensive timeline
Just four days after the deposition was released to the public — and with only six months to go before the 12-year statute of limitations for aggravated indecent assault runs out under Pennsylvania law — Montgomery County district attorney Risa Vetri Ferman reopened the Constand investigation. (As the prosecution has reiterated numerous times throughout the retrial, it was not Constand who went to the district attorney's office but the D.A. who approached her about reopening the case.)
That December, Cosby was charged with sexual assault.
At the Norristown news conference at which Ferman announced the charges, her successor, Kevin Steele, alluded to the deposition in his remarks. "A prosecutor's job is to follow evidence wherever it takes us, and sometimes that means whenever it comes to light," Steele said. "Reopening this case was not a question. Rather, reopening this case was our duty as law enforcement officers."
Trying — and failing — to keep Quaaludes out of the courtroom
Cosby's team has fought repeatedly to keep this deposition out of the courtroom.
Before his first criminal trial last June, Cosby's team argued that the deposition should be ruled inadmissible. They claimed that then-D.A. Bruce Castor promised Cosby he would never be prosecuted in the Constand case and that this promise extended to all future holders of the district attorney's office. That alleged agreement prompted Cosby's candor in the 2005 deposition, Cosby's team argued; the deal, which would help Constand's civil suit, meant that the testimony could never be deployed in a criminal case against Cosby.
But no written record of this agreement exists. Judge O'Neill ruled against the defense — who were also trying to get the entire case dismissed — and allowed the deposition to be admitted. (He did, however, reject the prosecution's request to bring up references to "Spanish fly," a drug-slash-aphrodisiac Cosby joked about giving girls in his youth.)
Bill Cosby Wants The Most Damning Piece Of Evidence Against Him Thrown Out
Even with that deposition, though, the prosecution couldn't land a conviction. They wound up with a hung jury and a mistrial.
As the retrial approached this spring, Cosby's new defense team, led by Thomas Mesereau, fought again to keep the deposition from court, and Judge O'Neill suggested at a pretrial hearing that his ruling might be different this time around. "This defendant is not on trial for what he said in his deposition," O'Neill said, announcing that he would issue no ruling on that testimony until it came up during the trial.
Tuesday morning, O'Neill ruled that the deposition could be admitted.
He said, she said, she said, she said, she said, she said, she said.
At the retrial, five women who also say Cosby drugged and sexually assaulted them were called to testify. That's a significant increase from the first trial, at which only one additional accuser, Kelly Johnson, was allowed to take the stand.
'Here was America's dad on top of me': Janice Dickinson takes the stand at the Cosby trial
"I just remember thinking how wrong it was. How very, very wrong it was."
The prosecution hopes to show that Constand, just like these five women — Heidi Thomas, Chelan Lasha, Janice Baker-Kinney, Janice Dickinson, and Lise-Lotte Lublin — was victimized by Cosby. That these accounts, taken together, prove Cosby regularly used drugs to incapacitate women he intended to assault.
In the deposition, Cosby is adamant that all his sexual liaisons were consensual. Except, that is, when he was asked about Therese Serignese.
Serignese was one of the 13 Jane Does in Constand's civil suit. She went public with her allegations in 2014, telling the Huffington Post that Cosby drugged and raped her in 1976.
"The next memory I have was I was in a bathroom and I was kind of bending forward and he was behind me having sex with me. I was just there, thinking 'I'm on drugs, I'm drugged.' I felt drugged and I was being raped and it was kind of surreal."
Was Serignese capable of consenting to sex after she took Quaaludes from Cosby?
Cosby's reply: "I don't know."
#Bill Cosby, #Criminal Justice, #Culture, #Justice, #Rape, #Sexual Assault


The 2018 Alabama baseball season opens up this weekend with a three-game series against Valparaiso as the Brad Bohannon era begins in Tuscaloosa.
First pitch for the season opener is set for 3:00 p.m. and it will be the first of seven straight home games to begin the year for the Crimson Tide. Whether you're able to make it to Sewell-Thomas Stadium or not, every fan can listen to live broadcasts for free through the Tide 102.9 app.
Friday's game will be one of a handful of exceptions this season. A few of the games will be played during live programming so Tide 102.9 will split and air local programming on the app and baseball on 102.9 FM in Tuscaloosa.
To download the app for iPhone or Android devices, visit this page. Once you download it, just click the listen button and you'll have Chris Stewart's live call of the game. We're also on Alexa devices so make sure you enable the Tide 102.9 skill and listen at home with a simple voice command.


To rent a renovated and furnished Studio with a living area of ca. 28 m² on the third floor of a listed building from the 16th century in the heart of the city. the cozy cottage is located a few steps from the lively shopping streets such as the Kalverstraat, Heiligeweg and several restaurants and Amsterdam pubs. The voetboogstraat is easily accessible by public transport and cycling.


text:
Q: How to perform Time Series Analysis on daily data? Sorry if my question is silly but I am extremely new to Data Science and Time series analysis.
So I have Tv program viewerships for the last 1 year and want to predict for the next 2 weeks. The data is of the form:
Year, Date, Week_day, Channel, Program, start_time, end_time, length, avg_impressions

avg_impressions is the average of all impressions of the program excluding the breaks. For example, if there were 10 impressions until first break, 20 impressions after first break until second and 30 impressions after the third break to the end, avg_impressions will be 20.
I want to do a time series analysis for the prediction. The data has 211,720 entries for 14 different channels. The data is from 10/10/2015 to 9/9/2016.
I am not very sure on how to convert the ``Impressions` into a time series object. I tried:
pts <- ts(train_agg$Impressions, start=c(2015, 10, 10), end=c(2016, 9, 9), frequency=357)

Is this format correct? I want to check for seasonality in the data. (I read I could tbats on the ts object for that).
Can someone please explain how to specify start dates and end dates for the vector? I read the answers from here, here and here but I am still not sure if I am doing it right.
My R code for what all i did till now:
train_data <- head(prog, 207595)
test_data <- tail(prog, 4126)
colnames(train_data)[12] <- "Impressions"
colnames(test_data)[12] <- "Impressions"
train_agg <- aggregate(Impressions~date_in_days, data=subset(train_data, Channel=="NBC" & Hour==19), mean)
test_agg <- aggregate(Impressions~date_in_days, data=subset(test_data,    Channel=="NBC" & Hour==19), mean)

pts <- ts(train_agg$Impressions, start=c(2015,10,10), end=c(2016,9,9), frequency=357)
plot.ts(pts)

pts.msts <- msts(pts,seasonal.periods=c(7,357))
model <- tbats(pts.msts)
plot(forecast(model, h=7))
forecast(model, h=7)
accuracy(model)

Is this the right way? I am totally lost. Can someone please give me pointers as to what I should do?

A: For starters, your data is too short.  When measuring seasonality(ie monthly) you need 3 iterations.  Can you get more data?  If not, then you are left to make a lot of assumptions which can be dangerous. Post your data and what country it is from and the beginning date.
By using regression, you can solve this problem. You can consider using 11 monthly dummies, 6 day of the week dummies and holiday dummies.  Not all may be significant.  Not all may be constant(ie june is high and then becomes low).  You need to look for outliers and build a dummy for them.  You need to look for lead and lag effects around holidays.  You need to consider day of the month impacts, week of the month impacts, long weekend, friday before a monday holiday, monday after a friday holiday.  You might have a trend or multiple trends.  You might have a change in the general volume called a level shift.


A fine example of a full spectrum Canadian Labradorite freeform cabochon. This would be ideal for a designer ring or a delicate pendant Carleton has hand cut this cabochon to display optimum flash. Flash orientation best viewed as shown in pictures.


text:
<?xml version="1.0" encoding="UTF-8"?>
<?xml-stylesheet type="text/xsl" href="./testcase.xsl"?>
<test_definition>
  <suite category="wrt-packertool2-android-tests" name="wrt-packertool2-android-tests">
    <set name="packertool2" type="pyunit">
      <testcase purpose="Check if packer tool work properly" type="Functional" status="approved" component="wrt-packertool2-android-tests" execution_type="auto" priority="P1" id="Crosswalk_Manifest_Check">
        <description>
          <test_script_entry>/opt/wrt-packertool2-android-tests/packertool2/manifesttest.py</test_script_entry>
        </description>
       </testcase>
    </set>
  </suite>
</test_definition>

redpajama_set_name: RedPajamaGithub


BEST PRICE EVER on Skylanders SuperChargers Dark Edition Starter Pack for Xbox One!
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SIG's SIGnature 25 roof waterproofing system has been awarded the BBA (British Board of Agrément) certification.
The quality accreditation is for SIG's own range of Torch-On roofing bituminous membranes. These comprise polymer-modified bitumen waterproofing membranes, insulation boards and a vapour control layer (VCL) for use on flat and pitched roofs with limited access.
Suitable for warm or cold roofs, the SIGnature Torch-On range is available in three underlay options, with the potential for either 20 or 25 year warranties.


text:
California housing bill offers local control to Sonoma County, cities statewide to boost homebuilding
With the statewide housing affordability crunch worsening, California lawmakers are taking another crack at passing landmark legislation to force cities and counties to loosen development rules to allow mid-rise apartments near transit hubs and townhouses in single- family home neighborhoods.
Such a measure stalled last year, but this time around the legislation introduced this week adds a key wrinkle giving communities two years to draft their own plans to boost high-density home construction.
The proposed legislation, Senate Bill 50, still would require municipalities to build apartment complexes, such as near the North Bay's SMART commuter rail line, and change zoning rules in single-family neighborhoods to allow for four-unit townhomes called fourplexes. Also, local development plans would need state approval and could not disproportionately concentrate new housing in low-income areas, among other things.
California is experiencing the lowest homeownership rate since the 1940s, said Sen. Mike McGuire, D-Healdsburg, who co-authored the legislation with Sen. Scott Wiener, D-San Francisco.
"We are in crisis mode," McGuire said in an interview. "There is no perfect solution, which is why we're advancing critical tools to help solve this crisis, to help expedite the development of ... housing connected to transit."
The legislation reintroduced Monday with revisions comes with a narrow time frame for approval. The deadline for the bill to pass the Senate is Jan. 31. A similar measure was shelved in legislative committee last spring, stalling what was viewed as the most radical proposal to address the escalating housing costs and availability of affordable homes statewide.
"We've heard loud and clear that cities want flexibility to implement this kind of legislation in a way that works best for them," Wiener said in a statement, alluding to the two-year window cities and counties would have to achieve increases in building apartments and homes under the bill.
Gov. Gavin Newsom has yet to weigh in this year on the legislation and his influence could go a long way to determine the fate of measure. McGuire Tuesday deflected a question about whether he thought Newsom soon would voice support for the amended legislation. The governor last year expressed disappointment when the similar bill stalled.
Smaller communities with fewer than 50,000 residents would be subject to less stringent development rules than cities larger than that but would still have to allow construction of fourplexes in single-family home neighborhoods. Santa Rosa and Petaluma, each with more than 50,000 people, would have to allow among other things building one floor higher than the maximum permitted if a housing development proposal is located within a half mile of a Sonoma-Marin Area Rail Transit station.
Santa Rosa City Council last year set a goal of having 500 new apartments under construction downtown by Nov. 2020.
"There's a lot of similarities, and I think it's right in line with what we're trying to do in Santa Rosa," assistant city manager David Guhin said of the amended Senate legislation. "Hopefully we can be a model, or a test case for these types of aggressive policies."
Sonoma County needs up to 30,000 new homes over the next five years to meet its housing needs, according to an estimate county officials gave last year. Yet from 2011 to 2018, only 5,000 new houses and apartments were built countywide — and the 2017 fires destroyed about 5,300 homes in a single day.
State officials reported last year that California needed more than 1.8 million new homes by 2025 to keep up with projected population growth. A 2016 study from McKinsey Global Institute, part of the prominent consulting firm McKinsey & Co., said California should build as many as 3.5 million new homes by 2025 — a goal that Newsom has adopted.
Meanwhile, the state's median single-family home price is $550,000, more than twice the national average. In Sonoma County, that median home price was $649,000 in November, the latest data available from the California Association of Realtors.
Petaluma City Councilman Mike Healy said the amended housing bill seemed like "baby steps in the right direction," but he remains wary how the legislation would affect the entire county.
The zoning changes proposed by Wiener and McGuire's legislation might make sense in San Francisco where residents of all income levels commonly rely on public rail like the Muni Metro, Healy said. That's not the case for SMART, a north-south rail line from Sonoma County airport to Larkspur in Marin County that only began service in August 2017 and is working to boost ridership.
"You don't ride it to school or you don't ride it to grocery shopping," Healy said of SMART. "At most, you go to work and back so it's more used for commuting."
It's unclear if Wiener and McGuire's legislation will garner sufficient support in the Senate, particularly among fellow Democrats who were among the most hostile to the plan.
Last April, Sen. Anthony Portantino, D-La Cañada Flintridge, shelved the earlier bill before a vote in the appropriations committee he chairs.
Portantino on Monday told the Los Angeles Times he hadn't yet seen Wiener's changes, but expressed concerns the new bill would prioritize market- rate housing instead of low-income development without ensuring new streets, sidewalks and other infrastructure would come alongside homebuilding.
The measure has to pass the appropriations committee before a vote can be taken on the Senate floor.
Sen. Bill Dodd, D-Napa, said he favors the amended legislation.
"The bottom line is that we need to do a better job building housing," Dodd said in a prepared statement. "I appreciate Senator Wiener's efforts to make it easier to build housing, especially affordable infill projects. His new amendments providing greater local control, while still holding local governments accountable, is a big step in the right direction."


text:
Home International The taxi driver has filed a lawsuit against Elon Musk's company Tesla
The taxi driver has filed a lawsuit against Elon Musk's company Tesla
Elon Musk Tesla Owner
A taxi driver has filed a lawsuit against Elon Musk Company Tesla
The taxi driver said that his car raced too much and the brakes did not work
One person was also killed in the crash
Paris – A taxi driver in France has filed a lawsuit against the world's richest man Elon Musk's electric car maker Tesla.
According to the Epost, the taxi driver's 'Tesla Model 3' car was destroyed in a terrible accident in December last year.One person was killed in the crash
The driver attributed the accident to a technical fault in the car and a lawsuit was filed against the company.According to the report, the car of the taxi driver went out of control and ran over the people and things standing on the side of the road.
The vehicle crushed several bicycles and collided with a van trampling a passerby and came to a halt. The passerby died on the spot
The taxi driver told police on the spot that his car raced too fast and the brakes did not work.
He said he was helpless in the driver's seat and the car was running on its own. Tesla has not yet commented on the matter.
Previous articlePakistan left India behind,The list of the happiest countries in the world continues
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By the autumn of 2024, portable electronic devices sold in the EU will require USB-C charging
The Dubai Virtual Assets Regulator has opened an office in the Metaverse.
PM announced to spend additional funds of Rs. 500 billion for...


text:
Mean Stinks! Amber Riley Takes On The Bullies
Issues/Pop Culture/Style
As one of the stars on the megahit show Glee, Amber Riley is the ultimate high school friend-in-the-head we all wish we had back in the day. She's fabulous, funny, fiercely loyal to her truest friends and has a voice so big and beautiful, words can hardly describe it. Mercedes rules! And the actress who plays her, Amber Riley, has become a true icon to Gleeks and to young women around the world who would love to have just a fraction of her talent and her self esteem.
Amber Riley plays the role of a high school student, and she knows all too well what kids are going through these days. High school is usually known as a rough road for many (it sure was for me), but dealing with bullies can be even more complicated in this age of the internet where anonymity gives people courage they'd never have otherwise. Instead of sitting by and just y'know – being a superstar – Amber's using her star power to call attention to the issue of bullying.
Secret Deodorant has created a new Facebook page dedicated to starting a "movement of nice." On Secret's Mean Stinks page, users are encouraged to share their own experiences with bullying, get advice from others who have been there and experienced that, and they can also help to support PACER's National Bullying Prevention Center by buying Secret deodorant products. And Amber Riley has partnered with Secret and Mean Stinks to lend a voice to the movement!
Amber Riley and I did a Q&A interview, so read on to find out about more about Mean Stinks, Glee, and her favorite plus size designers!
Afrobella: Amber, as a fellow plus-size women of color, let me just say how awesome it is to see you out there representing for us. Your style is always flawless. What is your best advice for plus size women trying to figure out a sense of unique style giving that sometimes there's limited options that your presented with?
Amber: First, thanks for the great compliment! I would say the best way to feel confident with yourself is to find something about yourself that you like, and accentuate it. So, if you like your butt, accentuate your butt, if you like your waist, find things that accentuate your waist, and wear your true size! Don't wear things that are too big for you, and don't wear things that are too small for you. Wearing your true size is so important. And, Spanx are a blessing – don't fight them, just wear them!
Afrobella: Do you have any favorite designers we have to look out for?
Amber: I will say that I love to shop when I go to London and one of the places I love to go to is Dorothy Perkins. I love this shop because they have a broad range of sizes. They go from a 4 to a size 24. I also like Style 3-6-9, which can also be found online – it's also London-based. They're with the Arcadia Group, a London-based clothing line. So those two clothing lines are my little secret weapons.
Afrobella: As a star on Glee, you get the best of both worlds. You get to show the world you can act and sing. Do you self identify yourself as an actress or as a singer?
Amber: I think that's like asking someone to choose between their children. I love them both and I feel like I'm good at both and I've grown up doing both. So I kind of just love them equally. I used to do one more than the other, I used to sing more than I acted, and now I'm acting more than I sing.
Afrobella: What are your favorite songs that you have performed on the show?
Amber: Oh gosh! My favorite song was probably "Aint No Way" that was a lot of fun to do because I felt like I sang it like me on the show, it was a little older for me to sing, so a great challenge. That was probably one of my favorites.
Afrobella: What advice do you have for teens who are being bullied right now and hating their high school experience?
Amber: I would tell them that first off, high school is only four years and that there is life after high school – I promise! Second, try to ignore the negativity and focus on the positive things in your life. Find activities outside of high school to get involved. If you like music, find a choir to get involved with. Find an outside outlet that distracts you from whatever is going on in school. And, if you are being bullied, tell a teacher, tell an adult, tell someone that can help you with the problem. Be proactive, not reactive; don't fight back with your words, go to someone that can actually help.
Afrobella: As a icon to teenagers around the world, tell me what the Mean Stinks Campaign means to you, and what you hope to achieve as part of it?
Amber: I think the great thing about Secret's Mean Stinks program is that it's giving girls an outlet to express how they feel about bullying and to get and give advice about bullying. If someone is being bullied, just getting information about how to deal with it is critical – how to deal with girl-on-girl combat, or deal with negativity is super important and that's what Mean Stinks on Facebook does. I get a lot of letters from girls and questions about negativity and now I can recommend this program as a resource. It's a good way for everyone to connect and figure out how to deal with it together as a group.
Thanks Amber, and thanks Secret! For more information about preventing bullying at schools, visit PACER.org/bullying or TeensAgainstBullying.org, or call 952-838-9000.
To join Secret's Mean Stinks program and support the movement of nice, click here to join on Facebook and click here to follow on Twitter.
Click here to follow Amber on Twitter – she's super sweet and talks to her fans! I must admit, I've got extra love for Amber because her middle name is Patrice 🙂
Did you ever face bullies in high school, bellas? What is your best advice for teenagers dealing with mean girls at school today? Are you a big Gleek and a fan of Amber Riley's? Tell me in the comments!
Tagged With: afrobella interview, celebrities, Interviews, Pop Culture
I love her and always look forward for her spotlights on the show. I look forward to seeing her on the new season of Glee and hope that her character is allowed to shine.
So I'm a huuuuuuuge fan of Amber Riley, I love the chaka khan covers she does on youtube. Thanks for this interview bella, you stay on top of the good stuff!
L'afrique cest chic says:
FYI you can shop dorothy perkins online: http://www.dorothyperkins.com
Mishara says:
Thank you so much for asking where she shops. I now loooooove Dorthy.Perkins after checking out the site for the first time yesterday.
Agatha Tefora says:
I also love Amber's chaka khan and other videos on youtube. She has a strong but pleasant voice. Amber is doing a great job on Glee. Among the Glee cast, she's my favorite . It's good to hear that she is supporting bullying prevention campaign.
Mommy622 says:
Great interview Bella, my daughter and I loved reading this one. Keep up the awesome work! Thanks for sharing with us.


text:
Q: Sidebar border not getting displayed I am writing a website code but I am unable to display the border for the sidebar. Here it is. And below is the code,
<!DOCTYPE html >

<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
<title>The New Boston</title>

Home

Home

Home

Home

Home

This is the complain area. Fill complains here

This is a website.

A: 1) Your html has a bug- you've given a/ instead of /a:

Home

2) float to the rescue: give this property in:

This is a website.

and add float: right to #sidbar
3) What is border: thin in #sidebar? Give border: 1px solid. Read this for allowed attributes and their values: http://www.w3schools.com/css/css_border.asp
UPDATE
Check here: http://jsfiddle.net/FPJTn/1/
The sidebar was breaking to the next line because of the value given for width for content. I have changed in #content css from width: 800px to width: auto.


Anirudh has mentioned that A R Rahman is a king and he is only just a minister. The film Kaththi has the music of Anirudh. This film has been trending in the I Tunes in India.
Anirudh is in cloud nine since the film Kaththi like his earlier films have topped the I Tunes list. Because of this Anirudh chatted with his Facebook friends at 6 pm yesterday.


Paul Cornell has had work produced and published since 1990 in all mediums, TV, Books and Comics.
Dr Who has featured prominently in his work (likewise in all media and audiobooks); he's also written numerous award-nominated comic series for both Marvel and DC – notably Captain Britain, Wolverine and Demon Knights – non-fiction works on popular TV shows (Dr Who included naturally!), and the Shadow Police novels, originally conceived as a TV show pitch overseen by Steven Moffat and others, and now brought to you by Tor books.
You can find more comprehensive lists of Paul's work on his website or Wikipedia page (above); we've highlighted key works with images below.
It's a busy second half of 2015 for Paul with his short story collection from NewCon Press – see here on our August New Book Recommends – and his novella Witches of Lychford, likewise on our September New Book Recommends here! He's also started a podcast (main links above), has the collection of Titan Comics Dr Who comics event The Four Doctors coming in December (currently publishing in comic form) and his new mature readers series This Damned Band out from Dark Horse Comics as well!
Paul Cornell is a writer of science fiction and fantasy in prose, comics and TV, one of only two people to be Hugo Award-nominated for all three media. He's written Doctor Who for the BBC, Action Comics for DC, and Wolverine for Marvel. He's won the BSFA Award for his short fiction, an Eagle Award for his comics, and shares in a Writer's Guild Award for his television.
Read an Extract / Preview of London Falling on Carabas here!
MARVEL: WOLVERINE Paul Cornell started out writing Doctor Who fan fiction, went on to write Doctor Who novels, audio plays and comics, and, having won a BBC contest to get a play on TV, and worked his way up through shows like Casualty and Holby City, became the fan who got to write for the show itself.
DC: DEMON KNIGHTS He went on to work on other TV series like Robin Hood and Primeval, to write two seasons of his own CITV show, Wavelength, and to break into comics, writing for Marvel, DC and 2000AD on such titles as Captain Britain and MI-13, Young Avengers, Wolverine, Knight and Squire, Batman and Robin,Action Comics, Demon Knights, XTNCT and Pan-African Judges. He created Saucer Country for Vertigo, and has new creator-owned projects in the pipeline.
In the meantime, he'd also achieved his ambition to become a novelist, first having two SF novels,Something More and British Summertime, published by Gollancz, then starting the Shadow Police series with London Falling and The Severed Streets at Tor.
DC: OTHER His short fiction, often featuring the character of Jonathan Hamilton, an out-of-uniform soldier in a parallel world where the 'great game' of European espionage continues into space, has been published in Isaac Asimov's Science Fiction Magazine, Interzone, The Daily Telegraph, The Timesand at Tor.com. He's also written twice for George R.R. Martin's Wild Cards short story anthologies.
Paul has also scripted Judge Dredd, Soldier Zero and (of course) Dr Who comics, and the Dr Who TV episodes "Father's Day" (2005), "Human Nature" (2007), "The Family of Blood" (2007). He's the creator of Bernice Summerfield, a Doctor Who companion from the novels who for twenty years now has had her own spinoff line of books and audio plays.
He lives in Buckinghamshire with his wife, a priest in the Church of England, and their toddler son, Thomas. His interests include cricket, matters Fortean and Kate Bush.


text:
Выборы депутатов Законодательного собрания Калужской области шестого созыва состоялись в Калужской области 13 сентября 2015 года в единый день голосования одновременно с выборами губернатора области.

Выборы прошли по смешанной избирательной системе: из 40 депутатов 20 были избраны по партийным спискам (пропорциональная система), другие 20 — по одномандатным округам (мажоритарная система). Для попадания в думу по пропорциональной системе партиям необходимо было преодолеть 5%-й барьер.

Ключевые даты
 20 мая 2015 года Избирательная комиссия Калужской области утвердила календарный план мероприятий по подготовке и проведению выборов.
 14 июня Законодательное собрание Калужской области назначило выборы на 13 сентября 2015 года (единый день голосования).
 17 июня постановление о назначении выборов было опубликовано в СМИ.
 с 24 июня по 17 июля — период выдвижения партийных списков и кандидатов по одномандатным округам от партий.
 с 29 июня — период самовыдвижения кандидатов.
 агитационный период начинается со дня выдвижения и заканчивается и прекращается за одни сутки до дня голосования.
 по 31 июля — период представления документов для регистрации кандидатов и списков.
 с 15 августа по 11 сентября — период агитации в СМИ.
 12 сентября — день тишины.
 13 сентября — день голосования.

Участники
5 политических партий получили право быть допущенными к выборам без сбора подписей избирателей:
 Единая Россия
 Коммунистическая партия Российской Федерации
 ЛДПР — Либерально-демократическая партия России
 Справедливая Россия
 Яблоко

Выборы по партийным спискам
По единому округу партии выдвигали списки кандидатов. Для регистрации выдвигаемого списка партиям требовалось собрать 0,5 % подписей от числа избирателей.

Выборы по округам
По 20 одномандатным округам кандидаты выдвигались как партиями, так и путём самовыдвижения. Кандидатам требовалось собрать 3 % подписей от числа избирателей соответствующего одномандатного округа.

Результаты

Примечания

Ссылки
 Избирательная комиссия Калужской области.
 Выборы депутатов Законодательного Собрания Калужской области шестого созыва. Единый день голосования 13 сентября 2015 года. Избирательная комиссия Калужской области.
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Калужская область
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How Can A Lung Injury In A Car Accident Effect My Quality of Life?
The lungs are incredibly fragile. Perhaps that's why humans evolved a whole system of muscles and bone in order to protect them. This system did an excellent job of protecting their vital organs when we were running after herds of antelope a thousand years ago, but being slammed into by a two-ton hunk of speeding metal is a whole different deal.
There are multiple ways of suffering a personal injury to the lungs in a car accident. Of course the obvious way is a head-on accident, but side-impact accidents and rear end accidents can also do serious damage to one or both lungs. This is generally caused by compression due to seat belts. This is not to say seat belts are bad, everyone should wear them every time they drive.
Lungs are not just open sacks that take in air, they are a complex system that separates CO2 and oxygen from air taken in so that the oxygen can be absorbed by the body for various functions, and then extract CO2. There are many components from the trachea to bronchus in separate lobes that can be damaged and can take a long time to heal.
When it comes to lung injuries in car accidents seat belts, steering wheels, and arm rests can have a major impact, and also chemicals and smoke. All of these can lead to different types of injuries.
Punctured Lung – Force from a car accident can be such that it breaks a rib and causes it to tear the lung deeply enough that it seeps air into the chest cavity putting painful pressure on the victim's body.
Collapsed Lung – Once a lung has been punctured, it can result in a collapsed lung (pneumothorax). This happens when there's a collection of air in the space around the lungs. This buildup of air puts pressure on the lung, so it cannot expand as much as it normally does when you take a breath and can cause suffocation if not treated right away.
Chemical Burn – Hundreds of different chemicals are used to create cars from the upholstery treatment to most of the components making up the engine. If the engine or consol catches fire, under the right circumstances the chemical fumes can burn the lungs before the smoke does. During a car accident that causes the air bags to deploy, the car can fill with a white powder. This powder is generally made up of either corn starch or talcum powder and is not usually detrimental, but can cause breathing problems for a few days if fully inhaled. However, it can last longer and have serious consequences for those with COPD and other lung function ailments.
Smoke Damage – Smoke is caused by burning material discharge and super heated particles that fill the air. During a car fire, some cars are better protected from burning the driver than others, but if the windows are rolled up, no car can remedy being filled with smoke surprisingly quick. If the victim in the car is rendered unconscious, even for a minute or two, they could suffocate. If they aren't, even disorientation (which is common after a car accident) can cause serious smoke inhalation. A fire can produce compounds that do damage by interfering with the body's oxygen use at a cellular level. The heat can also seriously burn the lungs causing internal scarring that could be permanent and inhibit breathing and activity level for the rest of the victim's life.
Physical damage to the lungs is not the only damage that the victim can suffer. These injuries take a ton of time and effort to heal. Not being able to breath well can cause the patient to lower their activity level. This can result in not only prolonging the problem and weight gain, but also depression, researchers have found.
Joseph Bienvenu, MD, PhD, of Johns Hopkins University, and colleagues reported in the American Journal of Respiratory and Critical Care Medicine found that about 40% of patients reported depressive symptoms and 66% reported impaired physical function during that time period.
"Incident depressive symptoms and incident impaired physical function are common and long-lasting during the first two years after acute lung injury," they wrote.
This was a longitudinal cohort study (a study that collects the same set of data over long periods then comparatively studied) of 186 acute lung injury patients from 13 intensive care units at four hospitals between October 2004 and October 2007. They were all survivors of acute lung injury.
Of all of the patients studied, a total of 21% had baseline depression. Futher, 40% had baseline impaired physical function. Over two years of follow-up after acute lung injury, the incidence of depressive symptoms in 147 at-risk patients was 40%, and the cumulative incidence of impaired physical function in 112 patients at risk was 66%, they found.
"Depressive symptoms are not only relatively persistent in acute lung injury survivors; they are also an independent risk factor for subsequent impairment in physical function," Bienvenu and colleagues wrote.
The investigators concluded that depressive symptoms are a significant and potentially modifiable risk factor for late-onset physical impairment, and that clinicians should run interventions that target these in order to improve long-term outcomes in survivors.
"Early identification and treatment of depressive states should be evaluated as a potential intervention to minimize the suffering and impairment that affect so many of these patients," they wrote.
Limitations of the study included self-report of depressive symptoms, use of medical records to identify baseline depression, and inability to account for interval treatment of either depression or physical function impairment.
A lung injury suffered in a car accident can last or years, even a lifetime, causing the victim to not be able to enjoy the activities that enriched their lives prior to the injury. This is particularly true in the Northwest that is known for hiking, mountain climbing, biking, kayaking, skiing, and other strenuous activities that require full breath control.
But this can also extend to doing your job or simply playing with your children. Without these things it effects your life at the most basic needs level. To overcome these life altering experiences, a victim needs the resources to gain as much of their prior lung capacity as possible through physical therapy, psychological therapy, and training (both physical and occupational). This takes money that insurance may not cover.
Phillips Law Firm is a local law firm with a substantial track record of success Personal Injury Litigation. We take the time to fully assess the injured party's case in order to assure that the victim receives the compensation they deserve. Call our Personal Injury Attorneys today for a free consultation.


Note: August marks the 2nd time this year that financial stress levels have reached an all-time low, with the previous instance occurring in May, when the Index dropped to 49.9. Further, our stress indicator has fallen 13% over the past year, as more Americans begin to feel in their own lives the effects of a re-surging economy, particularly in the form of higher wages.
Consumers' confidence in their ability to get a job and the overall economy are seen as important indicators of how freely they will spend, especially on big-ticket items such as cars, in coming months.
Consumer spending accounts for roughly 72 of US economic activity.
The overall economy, as measured by the gross domestic product, grew at a 4.1% rate in the April-June frame, the best performance since Y2014. That estimate will be revised Wednesday. Many economists believe growth has slowed a bit in the current quarter to around 3%, but will remain far ahead of the weak 2.2% GDP growth rate in the Q-1.
"Confidence is soaring to new heights which makes us bullish on growth and forecasts that this expansion may indeed shatter records for longevity next Summer," said the chief financial economist at MUFG Union Bank in New York.
Seeing Inflation Almost Everywhere We Look, Growth Too!


Here at Royal Rebels, you and your daughter can order gorgeous accessories that can put the finishing touches to any outfit. There are plenty of great clothes for girls to choose from here with us, but often it's only when the scarf, socks, hat and headband are put on that you really feel dressed. If your girl is interested in clothes and fashion, she will love the different kinds of accessories we sell here at Royal Rebels. Our accessories match perfectly with our clothing collections in terms of style, colours and patterns. A patterned scarf in vibrant shades can add a little colour to an otherwise toned-down outfit, and a pair of bold knee-highs can quickly pep up your daughter's favourite dress or skirt. Suited for both everyday and special occasions, we sell wonderful hair accessories such as clips and hair bands in beautiful floral patterns and in several different colours.


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Growth and Success Result in New Building
By bomla Leave a comment What's New
Under the direction of Dallas ISD Trustee Lew Blackburn, The Barack Obama Male Leadership Academy (BOMLA) will relocate to the A. Maceo Smith High School building at the close of the 2017-2018 school year. BOMLA has long outgrown its current space located at the B.F. Darrell campus on Lancaster Road since it opened in 2011. The FRIENDS of BOMLA advocated for a larger space and for BOMLA's inclusion in the Dallas ISD 2015 Bond.
The A. Maceo Smith campus is currently home to students and faculty of New Tech High School. Both campuses will move during the summer months, and renovation and construction will begin on both buildings. Dallas ISD has committed to investing $18 million to make the renovations, divided evenly between the two campuses. The A. Maceo facility features a nearly 1,000 seat auditorium, 2 gyms, sports fields, and capacity for 1,200 students. Both schools will reopen in their new spaces in August 2018 and renovations will continue throughout the school year.
"This is a great next step on our quest to give our young men an infrastructure that removes barriers" said Sophia Johnson, Friends of BOMLA Chair. "We desperately needed the space and this is the answer that removes barriers to learning and success."
BOMLA is slated to enroll more than 120 additional students next year, an increase of more than 31 percent from the previous year, which means that the move is happening at the right time.
Dallas ISD Trustee Dr. Lew Blackburn has confidence in BOMLA's trajectory towards success.
"Dallas ISD has proven that the BOMLA formula works" Blackburn said. "BOMLA students have achieved 100% graduation rates, outstanding academic scores, Blue Ribbon designation from the U.S. Department of Education and been exposed to character-building programs and experiences. Increasing BOMLA's enrollment capacity is an excellent investment in future leaders for our city, state, and nation. We look forward to BOMLA's continued success at the A. Maceo Smith campus."
BOMLA administration, FRIENDS of BOMLA, and the Dallas ISD Architecture and Design Team are working closely to ensure that BOMLA's unique program offerings and culture of leadership remained intact at the new site. For example, building redesign will encourage upper and middle school engagement and collaboration. FRIENDS will host tours for donors and community leaders beginning in Fall 2018. Follow @BOMLAFRIENDS on social media for updates.
Watch this story on WFAA!
Please note FRIENDS of BOMLA mailing address: P.O. Box 398455, Dallas TX 75339.
PHOTO: HKS Architects
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Josep Carreras Leukaemia Research Institute
Josep Carreras International Leukaemia Foundation
Administrative Transparency IJC page
IJC Sample Collection for Leukemia and other Hematolgical Diseases
Evenings at the Carreras
Scientific Founding Members
Management commission
Internal Scientific Committee
Coordinators of the 3 Campuses
Research Directors on the Campuses
Why a Research Institute focusing on Leukaemia & other malignant blood diseases?
IJC Campus Clinic-UB
Publication year [encoded data, 6959 characters]
Researcher ---Acha, PamelaAranda, JessicaAriza, LorenaBanús-Mulet, AntòniaBaptista, Maria JoaoBardina, JorgeBueno, ClaraBuschbeck, MarcusCabezón, MartaCarreras, EnricCasas, EduardCasquero, RaquelCastaño, JCid, EmiliCobo, IsabelCornet Masana, Josep MariaCorujo, DavidCouso, JorgeCufí, SDiesch, JeannineDouet, JulienDíaz de la Guardia, RafaelDíaz-Ricart, MaribelEtxabe, AmaiaFuster, FranciscoGarcia, OlgaGarcia, LauraGenesca, EulàliaGiorgetti, AlessandraGranada, IsabelGrau, JavierGuberovic, IvaHorrillo, AngelicaHurtado-Bagès, SarahJavierre, Biola MLara, Mari CarmenMalinverni, RobertoMallo, MarMarcé, SilviaMartín, BeatrizMenéndez, PabloMir, EnriqueMolina, OscarMoreno, MiriamMorán, ÉricaMuncunill, JosepMuñoz Risueño, RuthNavarro, TomásNomdedeu Fàbrega, MeritxellOriol, AlbertPalau, AnnaPalomo, LauraPalomo, MartaPasquali, LorenzoPetriz, JordiPrieto, CristinaRibera, JordiRibera, José MaríaRoca-Ho, HeleiaRodriguez, VirginiaRuiz, NeusSanchez, MaykaSancho, Juan ManuelSanjuan, AlejandraSerrano, EricaSolé, FrancescSorigue, MSuñe, GuillermoUrbano-Ispizua, ÁlvaroValero, VanesaVavouri, TanyaVives-Corrons, Joan LuisXandri, MarisolXicoy, BlancaYamamoto, FumiichiroYamamoto, MiyakoZamora, Lurdes
Only publications by the IJC
Found 44 publicacions matching the indicated search criteria.
Lo Re O, Douet J, Buschbeck M, Fusilli C, Pazienza V, Panebianco C, Castracani CC, Mazza T, Li Volti G, Vinciguerra M
Histone variant macroH2A1 rewires carbohydrate and lipid metabolism of hepatocellular carcinoma cells towards cancer stem cells.
Epigenetics 30 Aug 2018, . Epub 30 Aug 2018
Hepatocellular carcinomas (HCCs) contain a sub-population of cancer stem cells (CSCs) that are responsible for tumor relapse, metastasis, and chemoresistance. We recently showed that loss of macroH2A1, a variant of the histone H2A and an epigenetic regulator of stem-cell function, in HCC leads to CSC-like features such as resistance to chemotherapeutic agents and growth of large and relatively undifferentiated tumors in xenograft models. These HCC cells silenced for macroH2A1 also exhibited stem-like metabolic changes consistent with enhanced glycolysis. However, there is no consensus as to the metabolic characteristics of CSCs that render them adaptable to microenvironmental changes by conveniently shifting energy production source or by acquiring intermediate metabolic phenotypes. Here, we assessed long-term proliferation, energy metabolism, and central carbon metabolism in human hepatoma HepG2 cells depleted in macroH2A1. MacroH2A1-depleted HepG2 cells were insensitive to serum exhaustion and showed two distinct, but interdependent changes in glucose and lipid metabolism in CSCs: (1) massive upregulation of acetyl-coA that is transformed into enhanced lipid content and (2) increased activation of the pentose phosphate pathway, diverting glycolytic intermediates to provide precursors for nucleotide synthesis. Integration of metabolomic analyses with RNA-Seq data revealed a critical role for the Liver X Receptor pathway, whose inhibition resulted in attenuated CSCs-like features. These findings shed light on the metabolic phenotype of epigenetically modified CSC-like hepatic cells, and highlight a potential approach for selective therapeutic targeting.
Dumbovic G, Biayna J, Banús J, Samuelsson J, Roth A, Diederichs S, Alonso S, Buschbeck M, Perucho M, Forcales SV
A novel long non-coding RNA from NBL2 pericentromeric macrosatellite forms a perinucleolar aggregate structure in colon cancer.
Nucleic Acids Res. 20 Jun 2018, 46 (11) 5504-5524.
Primate-specific NBL2 macrosatellite is hypomethylated in several types of tumors, yet the consequences of this DNA hypomethylation remain unknown. We show that NBL2 conserved repeats are close to the centromeres of most acrocentric chromosomes. NBL2 associates with the perinucleolar region and undergoes severe demethylation in a subset of colorectal cancer (CRC). Upon DNA hypomethylation and histone acetylation, NBL2 repeats are transcribed in tumor cell lines and primary CRCs. NBL2 monomers exhibit promoter activity, and are contained within novel, non-polyA antisense lncRNAs, which we designated TNBL (Tumor-associated NBL2 transcript). TNBL is stable throughout the mitotic cycle, and in interphase nuclei preferentially forms a perinucleolar aggregate in the proximity of a subset of NBL2 loci. TNBL aggregates interact with the SAM68 perinucleolar body in a mirror-image cancer specific perinucleolar structure. TNBL binds with high affinity to several proteins involved in nuclear functions and RNA metabolism, such as CELF1 and NPM1. Our data unveil novel DNA and RNA structural features of a non-coding macrosatellite frequently altered in cancer.
Corujo D, Buschbeck M
Post-Translational Modifications of H2A Histone Variants and Their Role in Cancer.
Cancers (Basel) 27 Feb 2018, 10 (3) . Epub 27 Feb 2018
Histone variants are chromatin components that replace replication-coupled histones in a fraction of nucleosomes and confer particular characteristics to chromatin. H2A variants represent the most numerous and diverse group among histone protein families. In the nucleosomal structure, H2A-H2B dimers can be removed and exchanged more easily than the stable H3-H4 core. The unstructured N-terminal histone tails of all histones, but also the C-terminal tails of H2A histones protrude out of the compact structure of the nucleosome core. These accessible tails are the preferential target sites for a large number of post-translational modifications (PTMs). While some PTMs are shared between replication-coupled H2A and H2A variants, many modifications are limited to a specific histone variant. The present review focuses on the H2A variants H2A.Z, H2A.X, and macroH2A, and summarizes their functions in chromatin and how these are linked to cancer development and progression. H2A.Z primarily acts as an oncogene and macroH2A and H2A.X as tumour suppressors. We further focus on the regulation by PTMs, which helps to understand a degree of context dependency.
Palomo L, Malinverni R, Cabezón M, Xicoy B, Arnan M, Coll R, Pomares H, García O, Fuster-Tormo F, Grau J, Feliu E, Solé F, Buschbeck M, Zamora L
DNA methylation profile in chronic myelomonocytic leukemia associates with distinct clinical, biological and genetic features.
Epigenetics 2018, 13 (1) 8-18. Epub 6 Feb 2018
Chromosomal abnormalities are detected in 20-30% of patients with chronic myelomonocytic leukemia (CMML) and correlate with prognosis. On the mutation level, disruptive alterations are particularly frequent in chromatin regulatory genes. However, little is known about the consequential alterations in the epigenetic marking of the genome. Here, we report the analysis of genomic DNA methylation patterns of 64 CMML patients and 10 healthy controls, using a DNA methylation microarray focused on promoter regions. Differential methylation analysis between patients and controls allowed us to identify abnormalities in DNA methylation, including hypermethylation of specific genes and large genome regions with aberrant DNA methylation. Unsupervised hierarchical cluster analysis identified two main clusters that associated with the clinical, biological, and genetic features of patients. Group 1 was enriched in patients with adverse clinical and biological characteristics and poorer overall and progression-free survival. In addition, significant differences in DNA methylation were observed between patients with low risk and intermediate/high risk karyotypes and between TET2 mutant and wild type patients. Taken together, our results demonstrate that altered DNA methylation patterns reflect the CMML disease state and allow to identify patient groups with distinct clinical features.
Palau A, Garz AK, Diesch J, Zwick A, Malinverni R, Valero V, Lappin K, Casquero R, Lennartsson A, Zuber J, Navarro T, Mills KI, Götze KS, Buschbeck M
Polycomb protein RING1A limits hematopoietic differentiation in myelodysplastic syndromes.
Oncotarget 29 Dec 2017, 8 (70) 115002-115017. Epub 1 Dec 2017
Genetic lesions affecting epigenetic regulators are frequent in myelodysplastic syndromes (MDS). Polycomb proteins are key epigenetic regulators of differentiation and stemness that act as two multimeric complexes termed polycomb repressive complexes 1 and 2, PRC1 and PRC2, respectively. While components and regulators of PRC2 such as ASXL1 and EZH2 are frequently mutated in MDS and AML, little is known about the role of PRC1. To analyze the role of PRC1, we have taken a functional approach testing PRC1 components in loss- and gain-of-function experiments that we found overexpressed in advanced MDS patients or dynamically expressed during normal hematopoiesis. This approach allowed us to identify the enzymatically active component RING1A as the key PRC1 component in hematopoietic stem cells and MDS. Specifically, we found that RING1A is expressed in CD34
Marjanović MP, Hurtado-Bagès S, Lassi M, Valero V, Malinverni R, Delage H, Navarro M, Corujo D, Guberovic I, Douet J, Gama-Perez P, Garcia-Roves PM, Ahel I, Ladurner AG, Yanes O, Bouvet P, Suelves M, Teperino R, Pospisilik JA, Buschbeck M
MacroH2A1.1 regulates mitochondrial respiration by limiting nuclear NAD(+) consumption.
Nat. Struct. Mol. Biol. 9 Oct 2017, . Epub 9 Oct 2017
Histone variants are structural components of eukaryotic chromatin that can replace replication-coupled histones in the nucleosome. The histone variant macroH2A1.1 contains a macrodomain capable of binding NAD(+)-derived metabolites. Here we report that macroH2A1.1 is rapidly induced during myogenic differentiation through a switch in alternative splicing, and that myotubes that lack macroH2A1.1 have a defect in mitochondrial respiratory capacity. We found that the metabolite-binding macrodomain was essential for sustained optimal mitochondrial function but dispensable for gene regulation. Through direct binding, macroH2A1.1 inhibits basal poly-ADP ribose polymerase 1 (PARP-1) activity and thus reduces nuclear NAD(+) consumption. The resultant accumulation of the NAD(+) precursor NMN allows for maintenance of mitochondrial NAD(+) pools that are critical for respiration. Our data indicate that macroH2A1.1-containing chromatin regulates mitochondrial respiration by limiting nuclear NAD(+) consumption and establishing a buffer of NAD(+) precursors in differentiated cells.
Lo Re O, Fusilli C, Rappa F, Van Haele M, Douet J, Pindjakova J, Rocha SW, Pata I, Valčíková B, Uldrijan S, Yeung RS, Peixoto CA, Roskams T, Buschbeck M, Mazza T, Vinciguerra M
Induction of cancer cell stemness by depletion of macrohistone H2A1 in hepatocellular carcinoma.
Hepatology 15 Sep 2017, . Epub 15 Sep 2017
Hepatocellular carcinomas (HCC) contain a sub-population of cancer stem cells (CSCs), which exhibit stem-cell like features and are responsible for tumor relapse, metastasis, and chemoresistance. The development of effective treatments for HCC will depend on a molecular-level understanding of the specific pathways driving CSC emergence and stemness. MacroH2A1 is a variant of the histone H2A and an epigenetic regulator of stem cell function, where it promotes differentiation and, conversely, acts as a barrier to somatic cell reprogramming. Here we focused on the role played by the histone variant macroH2A1 as a potential epigenetic factor promoting CSC differentiation. In human HCC sections we uncovered a significant correlation between low frequencies of macroH2A1 staining and advanced, aggressive HCC subtypes with poorly-differentiated tumor phenotypes. Using HCC cell lines we found that shRNA-mediated macroH2A1 knock-down induces acquisition of CSC-like features, including the growth of significantly larger and less-differentiated tumors when injected into nude mice. MacroH2A1-depleted HCC cells also exhibited reduced proliferation, resistance to chemotherapeutic agents, and stem-like metabolic changes consistent with enhanced hypoxia responses and increased glycolysis. The loss of macroH2A1 increased expression of a panel of stemness-associated genes, and drove hyper-activation of the NF-κBp65 pathway. Blocking phosphorylation of NF-κBp65 on Ser536 inhibited the emergence of CSC-like features in HCC cells knocked-down for macroH2A1.
Douet J, Corujo D, Malinverni R, Renauld J, Sansoni V, Marjanović MP, Cantari'o N, Valero V, Mongelard F, Bouvet P, Imhof A, Thiry M, Buschbeck M
MacroH2A histone variants maintain nuclear organization and heterochromatin architecture.
J. Cell. Sci. 10 Mar 2017, . Epub 10 Mar 2017
Genetic loss-of-function studies in development, cancer and somatic cell reprogramming have suggested that the group of macroH2A histone variants might function through stabilizing the differentiated state by a yet unknown mechanism. Here, we present results demonstrating that macroH2A variants have a major function in maintaining nuclear organization and heterochromatin architecture. Specifically, we find that a substantial amount of macroH2A is associated with heterochromatic repeat sequences. We further identify macroH2A on sites of interstitial heterochromatin decorated by H3K9me3. Loss of macroH2A leads to major defects in nuclear organization including reduced nuclear circularity, disruption of nucleoli and a global loss of dense heterochromatin. Domains formed by repeat sequences when depleted of macroH2A are disorganized, expanded and fragmented and mildly re-expressed. On the molecular level we find that macroH2A is required for the interaction of repeat sequences with the nucleostructural protein Lamin B1. Taken together our results argue that a major function of macroH2A histone variants is to link nucleosome composition to higher order chromatin architecture.
Palau A, Mallo M, Palomo L, Rodríguez-Hernández I, Diesch J, Campos D, Granada I, Juncà J, Drexler HG, Solé F, Buschbeck M
Immunophenotypic, cytogenetic, and mutational characterization of cell lines derived from myelodysplastic syndrome patients after progression to acute myeloid leukemia.
Genes Chromosomes Cancer Mar 2017, 56 (3) 243-252. Epub 21 Nov 2016
Leukemia cell lines have been widely used in the hematology field to unravel mechanistic insights and to test new therapeutic strategies. Myelodysplastic syndromes (MDS) comprise a heterogeneous group of diseases that are characterized by ineffective hematopoiesis and frequent progress to acute myeloid leukemia (AML). A few cell lines have been established from MDS patients after progression to AML but their characterization is incomplete. Here we provide a detailed description of the immunophenotypic profile of the MDS-derived cell lines SKK-1, SKM-1, F-36P; and MOLM-13. Specifically, we analyzed a comprehensive panel of markers that are currently applied in the diagnostic routine for myeloid disorders. To provide high-resolution genetic data comprising copy number alterations and losses of heterozygosity we performed whole genome single nucleotide polymorphism-based arrays and included the cell line OHN-GM that harbors the frequent chromosome arm 5q deletion. Furthermore, we assessed the mutational status of 83 disease-relevant genes. Our results provide a resource to the MDS and AML field that allows researchers to choose the best-matching cell line for their functional studies. © 2016 Wiley Periodicals, Inc.
Buschbeck M, Hake SB
Variants of core histones and their roles in cell fate decisions, development and cancer.
Nat. Rev. Mol. Cell Biol. 1 Feb 2017, . Epub 1 Feb 2017
Histone variants endow chromatin with unique properties and show a specific genomic distribution that is regulated by specific deposition and removal machineries. These variants - in particular, H2A.Z, macroH2A and H3.3 - have important roles in early embryonic development, and they regulate the lineage commitment of stem cells, as well as the converse process of somatic cell reprogramming to pluripotency. Recent progress has also shed light on how mutations, transcriptional deregulation and changes in the deposition machineries of histone variants affect the process of tumorigenesis. These alterations promote or even drive cancer development through mechanisms that involve changes in epigenetic plasticity, genomic stability and senescence, and by activating and sustaining cancer-promoting gene expression programmes.
Josep Carreras Leukaemia Research Institute (IJC)
The IJC is a CERCA center.


A cruiser on the Norfolk Broads, the boat "Swan Renown" is a cruiser belonging to the boat-yard Swancraft Cruisers, and is good for exploring the rivers around Brundall. Swan Renown sleeps 4 people, with the bedrooms laid out as 2 double or 1 double and2 single. You can steer this cruiser from a centre steering position. 240v system on boat. Charge your mobile phone with the 12v socket. You have heating on Swan Renown. All the bed linen is available on Swan Renown. There is a vacuum cleaner on Swan Renown. Fitted carpetsYou can watch TV while staying on this boat. You can watch some DVDs while staying on this boat. You can bring your CD collection with you, as this boat has a CD player. Cruise while listening to the radio. Keep your hair in good order with the hairdryer. You get bed linen and duvets on Swan Renown. No need to let your hygiene slip - there is a shower on board. Rent a dinghy with Swan Renown. You have a bow thruster on Swan Renown to make the steering easier in those tight spots.
Swan Reflection - at Swancraft Cruisers, in Brundall.
Swan Regal - at Swancraft Cruisers, in Brundall.
Swan Rapture - at Swancraft Cruisers, in Brundall.
Swan Rapier - at Swancraft Cruisers, in Brundall.
Swan Radiance - at Swancraft Cruisers, in Brundall.
Swan Roamer - at Swancraft Cruisers, in Brundall.
Swan Rapide - at Swancraft Cruisers, in Brundall.
Swan Rhapsody - at Swancraft Cruisers, in Brundall.
Swan Raider - at Swancraft Cruisers, in Brundall.
The Cruiser above is Swan Renown - in the Swancraft Cruisers region of Brundall. We've also listed several other boats in Swancraft Cruisers; or for a bigger choice, look at all the boats available in Brundall. Hopefully you'll find something to suit your holiday needs!


Dr David Rendall is an internationally renowned speaker and author of several books (including The Freak Factor, The Four Factors of Leadership and Pink Goldfish) that explore the idea that performance can be lifted when imperfections and flaws are accentuated rather than suppressed.
Prior to becoming a speaker, David was a leadership professor and stand-up comedian, a wonderful combination that allows him to convey complex phycological propositions in an entertaining manner. David will be speaking on worldwide transformational change.


Chronic overproduction of growth hormone in cats is most often caused by a GH-producing tumour of the pituitary gland. In dogs the usual cause is quite different. In this species endogenous progestins and exogenous progestins cause excessive GH secretion that is reversible.
In October 2003 a 6.5-year-old intact female terrier with features of acromegaly was referred to small animal hospital of Tehran university. She had a visible increase in soft tissue mass, respiratory stridor, enlargement of the interdental spaces, prominent skin folds, abdominal enlargement and radiographic evidence of an increase in soft tissue mass in the oropharyngeal and pyometra. For a conclusive diagnosis was demonstrated elevation of GH levels (153.9ng/ml). Plasma Glucose level was 108 mg/dl and she had not any signs of polyuria and polydipsia. Also SAP level was high and PCV was low.
This animal was treated with progesterone to prevent estrus from 2 years ago. The history of frequent and excessive administration of a progestational drug induced acromegaly and pyometra. Progesterone withdrawal and ovariohysterectomy in this case was resulted in a reduction of plasma GH and appreciable clinical improvement. As well as E.coli was separated from uterus discharges.
It is concluded that treatment of dogs with synthetic progestins can induce the overproduction of GH in the mammary gland. This GH is biologically active, highly similar to pituitary derived GH, and originates from foci of hyperplastic ductular epithelium of the mammary gland. This results also suggested that acromegaly in this dog was caused by medroxyprogesterone acetate-evoked GH elevation.


Despite many clinical decision support systems (CDSSs) being rated as highly usable, CDSSs have not been widely adopted in clinical practice. We posit that there are factors aside from usability that impact adoption of CDSSs; in particular we are interested in the role played by MDs intrinsic motivation to use computer-based support. Our research aim is to investigate the relationship between usability and intrinsic motivation in order to learn about adoption of CDSS in clinical practice.
Following the evaluation of a CDSS, 19 MDs completed a 2 part questionnaire about their intrinsic motivation to use computer-based support in general and the usability of the evaluated CDSS.
The analysis of MDs motivation to use computer-based support demonstrated that MDs are comfortable using computer-based support and in general find using it quite easy (a motivation rating of 0.66 on a (0, 1) scale was computed). However MDs also reported a perceived lack of competence associated with a lack of prior experience using technology in practice, which results in pressure and tension. The considered CDSS scored highly on all usability dimensions and a usability rating of 0.74 was recorded. The examination of the relationship between motivation and usability suggested that users who were motivated to use computer-based support experienced better usability than those who reported low levels of motivation.
Our small case study suggests that an important factor supplementing the usability of CDSSs is intrinsic motivation to use computer-based support in general. We posit that the lack of such a measure thus far in CDSS evaluation may to some extent explain seeming MD satisfaction with CDSSs on one hand, but their limited adoption on the other. We recommend that clinical managers responsible for deploying CDSS should invest in training MDs to use technology underlying computer-based support applications instead of focusing only on the features of the specific CDSS to be deployed.