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The Jersey Council of the Institute of Chartered Secretaries and Administrators (ICSA) has announced today that Daniel William Finkelstein (Baron Finkelstein) OBE will be the guest speaker at the forthcoming ICSA Jersey Annual Dinner, Awards & Graduation Ceremony. An award-winning journalist and a renowned weekly columnist, Daniel is also a former Executive Editor and now Associate Editor of The Times.
After serving as director of a Westminster think tank focusing on social policy, he became a political advisor to then Prime Minister John Major and later William Hague. He was awarded an OBE for his governmental services in the 1997 honours list.
Daniel was consecutively named 'Political Commentator of the Year' in 2011 and 2012 at the Editorial Intelligence Comment Awards. The following year he was made life peer and given the title of Baron Finkelstein of Pinner, Middlesex.
Daniel's visit to Jersey has been kindly sponsored by ZEDRA.
Julie Fairclough, Director, ZEDRA Jersey, said: "ZEDRA are proud to be a part of this year's ICSA Awards. The ceremony celebrates the achievements of outstanding ICSA students as well as 125 years of ICSA championing good governance. This prestigious event is by far one of the most important ceremonies in the local business calendar.
"We are delighted to have been instrumental in bringing Daniel Finkelstein over to the Island as guest speaker this year. As a prominent commentator, Daniel will undoubtedly provide a sharp and entertaining insight into life led on the political frontline. His insider experience in a transitional political world has gained Daniel a wealth of knowledge that will be invaluable to both ICSA students and local business owners alike.
"The importance of good governance remains pivotal during any period of transition, now more than ever we look forward to learning from Daniel's unique experience."
ICSA Jersey Chairman, Robert Le Corre added: "We are very grateful to ZEDRA for making Daniel's visit to Jersey possible. We look forward very much to listening to his unique insights and observations, no doubt with a number of entertaining anecdotes along the way.
"Together with the recognition of academic achievement of our students and the announcement of the winners of the ICSA Jersey Awards 2016, Daniel's speech will mark the pinnacle of our 125 year anniversary celebrations."
The popular and prestigious black-tie ICSA Jersey Annual Dinner, Awards & Graduation Ceremony is sponsored by BPP Professional Education, Brainloop, Rowlands Recruitment, Sator Regulatory Consulting, and ZEDRA, and will be held at the Hotel de France on Friday, 30th September 2016.
The event is a sell-out with a waiting list open for anyone who has been unable to secure a ticket. For more information, please see the ICSA Jersey website at www.icsajersey.org.je or email events@immediateimpact.je
posted 27 Mar 2015, 07:11 by Icsa Admin [ updated 5 May 2016, 06:33 ]
View highlights from the 2016 Jersey conference
posted 23 May 2014, 02:04 by Icsa Admin [ updated 20 Sep 2016, 11:31 ]
About BPP Professional Education – 'Student Excellence' Award Sponsor
BPP Professional Education is a leading specialist provider of professional education, delivering a range of industry-leading Professional Qualifications, Professional Development programmes and Learning Media. They enjoy a trusted provider status for many of their clients and institutes and offer professionals opportunities to progress through a variety of qualifications in accountancy, tax, trust, insolvency, banking, finance and many more.
In Jersey, they have a dedicated team who have been helping staff realise their ambitions for over 16 years. In 2016, the Jersey team's commitment to delivering the best service possible to students and clients was recognised at the BPP Annual Awards where BPP Jersey was awarded 'Team of the Year'.
W: www.bpp.com | E: jerseyenquiries@bpp.com | T: +44 (0) 1534 711 800
About Brainloop – 'The One to Watch' Award Sponsor
Founded in 2000, Brainloop is a leading provider of highly secure Corporate Governance and Collaboration Solutions for managing confidential information and sharing documents internally and externally. Brainloop Secure Dataroom is a single platform for board, executive and fund administration teams to manage communications efficiently and securely.
Since the Panama leaks, protecting information has risen up the agenda and has never been more important. It must therefore be re-assuring that more than 200 financial services organisations, including the lead financial regulator in the UK trust Brainloop. They achieve greater productivity without the need to worry about security – annotations are never lost, Microsoft, Apple and Android tablets are no problem, working directly from MS Office is key, document versioning and PDF conversion are automated, board packs are easily created, delivered securely to directors' devices and updated; corporate compliance issues are met fully and regulatory requirements fulfilled.
W: www.brainloop.com | E: london@brainloop.com | T: +44 (0) 207 183 8285
About Rowlands – Our Drinks Reception Sponsor
Rowlands offer a confidential, friendly and contemporary recruitment service that specialises in the offshore finance sector. Working closely with an extensive range of some of the best finance firms, and with a comprehensive market knowledge developed over 40 years, they aim to support the career aspirations of all of their clients, making their job search easier and more rewarding.
"Many of our current vacancies require the highly regarded ICSA qualification which is why we are so delighted to be a sponsor of the ICSA awards," commented MD, Jeralie Pallot.
W: www.rowlands.co.uk | E: jobs@rowlands.co.uk | T: +44 (0) 1534 626 777
About Sator – 'Governance Team of the Year' Award Sponsor
Sator Regulatory Consulting Limited is a Jersey based company, wholly owned by Helen Hatton, who has over 20 years' director level experience in offshore financial services regulation and financial crime investigation.
Sator Regulatory Consulting offers international standard setters, regulatory authorities, financial services businesses and the DNFBP/NPO sector reviews, investigation, advisory and assessment assistance, or an additional resource on a consultancy basis.
This triangulation of experience – global standards, regional requirements and grass roots implementation greatly enhances the company's ability to offer its services in a focussed and applied context – global to local – risk adjusted and relevant.
W: www.sator.je | E: e.hatton@sator.je | T: +44 (0) 1534 617 298
About ZEDRA – Our Guest Speaker Sponsor
ZEDRA is an independent, global specialist in trust, fiduciary, corporate and fund services. The company was acquired from Barclays in January 2016 by an independent investor group, with an ambitious plan to grow the company, expanding and strengthening the services it offers to clients around the world.
Based in offices across eight key jurisdictions, its 300-strong team of industry experts is dedicated to creating and delivering bespoke solutions for clients. The business is focused on the strong commitment of an experienced team, fostering an entrepreneurial approach to delivering exceptional client services.
ZEDRA has a loyal client base of high-net-worth individuals and their families, international corporations and institutional investors. The company has the boldness and expertise to take on today's global challenges in an ever-evolving regulatory environment.
W: www.zedra.com | E: ashley.cox@zedra.com | T: +44 (0) 1534 873 741
posted 26 Jan 2014, 14:33 by Icsa Admin [ updated 27 Mar 2015, 07:12 ]

redpajama_set_name: RedPajamaCommonCrawl


Okay, I've played with FileMaker 5 and have been impressed. I'd like to publish a few databases on the web but I'm finding the "Instant Web Publishing" limitations unacceptable.
I've worked with GoLive 4 and found it a useful tool. With GoLive 5 now available, it provides some useful upgrades.
I've got a Beige G3 300Mhz machine with a static IP hooked to the University backbone.
Is someone using GoLive5 with FM Pro Unlimited on a Mac? Is it reasonable to combine these tools to create educational college level courseware for survey research classes?
Can you recommend some useful resources that will enable efficient integration of these tools and equipment?
I don't know GoLive (I use Dreamweaver) but I can tell you the best software I know of to ease building web enabled database systems with FileMaker's Web Companion: Home Page 3. I think that FileMaker Inc still sells it. You might check out their web site at http://www.filemaker.com.
If you want to go past Web Companion and use Lasso, Blueworld (the publishers of Lasso) offer a Lasso Studio for Go Live which adds some functionality that makes it easier to build web sites for Lasso and FileMaker, but I don't think it would be of much use for Web Companion.
I am using GoLive since version 2 and FM since version 1.
This combo is the best I've ever tried. I am using that on Macs and Windows.
I think Lasso was sort of necessary with v 4 of FM.
Version 5 Unlimited is very fast and I do not feel Lasso can improve something.


text:
Justin Theroux Discussed a Rumor About His Split From Jennifer Aniston
"That's a narrative that is not true, for the most part."
InStyle Editors
Our editors and writers comprise decades of expertise across the beauty, fashion, lifestyle and wellness spaces in print and digital. We prioritize journalistic integrity, factual accuracy, and also having fun with every story we share.
InStyle's editorial guidelines
Published on April 15, 2021 @ 09:53AM
Justin Theroux is addressing a rumor about the reason for his breakup with Jennifer Aniston.
In an interview with Esquire, Theroux shut down speculation that the couple split because she wanted to live in Los Angeles and he wanted to be in New York.
"That's a narrative that is not true, for the most part," Theroux said. "Look, people create narratives that make themselves feel better or simplify things for them. That whole 'This person likes rock 'n' roll, that person likes jazz. Of course!' That's just not the case. It's an oversimplification."
Theroux and Aniston first began dating in 2011, and married in 2015 before announcing their split in early 2018.
"I would say we've remained friends," he told Esquire about his relationship with Aniston. "We don't talk every day, but we call each other. We FaceTime. We text."
"Like it or not, we didn't have that dramatic split, and we love each other," he added. "I'm sincere when I say that I cherish our friendship. We can not be together and still bring each other joy and friendship. Also, she makes me laugh very, very hard. She's a hilarious person. It would be a loss if we weren't in contact, for me personally. And I'd like to think the same for her."
Justin Theroux Shared a Throwback Photo for Jennifer Aniston's Birthday
Since their split, Theroux has shared multiple public birthday tributes to Aniston, and in 2019, the ex-couple spent Thanksgiving together along with some of their friends, including Jimmy Kimmel, Jason Bateman, and Courteney Cox.
"I think that when you get good at relationships — and here I am, single — if you love the person the same way you loved them in the relationship, it would behoove you to love them the same way out of the relationship," he told Esquire. "Who wants to take a s— while you're walking out the door?"
Jennifer Aniston Said Vince Vaughn "Brought Me Back to Life" After Brad Pitt Divorce
Jennifer Aniston Wished Justin Theroux a Happy Birthday With a Shirtless Snapshot
Jenny Slate on Chris Evans: It's Hard Dating Captain America
Jennifer Aniston and Courteney Cox Had a Twinning Moment
Jennifer Aniston and Justin Theroux Reunited in a Sweet New Photo
Robert Pattinson Described FKA Twigs's Racist Trolls as "Demons Who Live in Basements"
Jimmy Kimmel Said He and Ex-Girlfriend Sarah Silverman Are "Like Brothers"
Norman Reedus "Didn't Want to Look at" Helena Christensen When He First Met Her
Jake Gyllenhaal Finally Shared His Thoughts on Taylor Swift's "All Too Well" Re-Release
Katy Perry and Russell Brand Had a Cat Named After Both of Them
Jon Peters Said Barbra Streisand Was "Probably" the Love of His Life
A Complete Timeline of Cardi B and Offset's Relationship
Kid Rock Said Getting Married to Pamela Anderson Was a "Blast" but "Being Married Sucks"
Penn Badgley Said He Was in "Earth-Shattering Love" With Zoë Kravitz
Jennifer Aniston Shared a Rare Update on Her Love Life
Olivia Munn Asked Aaron Rodgers "So What Do You Do?" When She Met Him


text:
Kristy Sandoval (born 1983) is a Xicana muralist based out of the Northeast San Fernando Valley of Los Angeles, California.

Background
She has painted over fifty murals since the start of her art career and has painted murals in Turkey. Sandoval is a featured artist and founder of Pacoima's Mural Mile and also works as Program and Volunteer Manager for Casa Esperanza. In 2017 she worked as a spokesperson for the NFL's Los Angeles Rams in collaboration to promote "Mi Cuidad, Mi Eqipo, Mis Rams" and celebrate Hispanic Heritage month. Notable award was in 2016 received the Phenomenal Woman Award by the Department of Gender & Women's Studies at Cal State University Northridge.

Sandoval studied at the Academy of Art University, receiving her BA in Web Design and New Media (2002-2007)

Art

 Decolonized 2015 is a mural part of Pacoima's Mural Mile. It features a woman who has blue hairs in the style of dreads and a tattoo on her left arm of Aztec Moon Goddess Coyolxauhqui. She uses a window with bars over it to create a cage image that has parrots and butterflies being set free.

 Being human, human being 22' x 50' is a mural that shows two crows perched on a bones with a background representing a serape. This piece is located in (Kadikoy) Istanbul, Turkey and was painted during the Mural Istanbul Festival (2015) where Sandoval was the first woman to participate.
 Assada Shakur 15' x 45' is another mural located on Pacoima's Mural Mile. This mural features Assata Shakur, an African American Freedom Fighter, author and political activist surrounded by flowers and the phrase "A Womyn's Place is in the Struggle". Sandoval was intentional in her spelling to try and show support to women with showing the word man is not needed to define them. She painted this mural as a collaboration with the HOOD Sisters.

References

Further reading

 Carter, Ryan. "Pacoima Artist, Teacher, and Now Promotora, Kristy Sandoval Uses Area's Murals to Promote Public Health." Daily News, Daily News, 28 Dec. 2020, https://www.dailynews.com/2020/12/27/pacoima-artist-teacher-and-now-promotora-kristy-sandoval-uses-areas-murals-to-promote-public-health/.
 Langlois, Jessica. "For Artist Kristy Sandoval, Murals Can Be a Form of Feminist Activism." LA Weekly, 22 May 2019, https://www.laweekly.com/for-artist-kristy-sandoval-murals-can-be-a-form-of-feminist-activism/.
 Morgan, Ewan. "A Look into the Future: The Los Angeles Olympics in 2028." KCET, 3 Aug. 2021, https://www.kcet.org/shows/lost-la/a-look-into-the-future-the-los-angeles-olympics-in-2028.
 Paez-Pumar, Luis. "The La Rams Worked with a XICANA Muralist to Celebrate Hispanic Heritage Month the Right Way." Remezcla, 16 Aug. 2017, https://remezcla.com/sports/xicana-muralist-los-angeles-rams/.
Braswell, Mary. "Climate Research Brightens an Elementary School Campus." UCLA Luskin, 18 May 2021, https://luskin.ucla.edu/climate-research-brightens-an-elementary-school-campus.

External links

Wikipedia Student Program
1983 births
Living people
American people of Mexican descent
American muralists
21st-century American artists
Academy of Art University alumni
Artists from Los Angeles
Women muralists

redpajama_set_name: RedPajamaWikipedia


Published at Tuesday, February 12th, 2019 - 03:46:55 AM. Bathroom. By Lionell Voisin.
There is simply no reason at all why a portable fan cannot be far out gorgeous, full of style, and work as a charm all at the exact same moment. Also remember a range-top exhaust fan is not going to help eliminate carbon monoxide unless it is vented outside. It is simpler to keep up with one fan instead of many. Exactly like their vintage counterparts, the vintage style fan is not always simple to discover. Picking out the appropriate size ceiling fan is not going to only maximize its operating potential, additionally, it impacts the way that it will appear in a particular space.
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Alex teaches classes on Wednesday evenings and is available for Private 1:1 Sessions at The Body Control Pilates Centre.
Alex found Pilates a welcome escape from the stress of a demanding marketing role in the City. Having tried many different fitness regimes, she started classes with a Body Control teacher and found that it was the one commitment in her week that she actively looked forward to. Before long she started to see and feel the incredible psychological and physical benefits and preached to anyone who would listen! It became a big part of her life and with this passion came the need to know more. The idea of being able to teach and help others blossomed and Alex went on to complete her teacher training with Body Control Pilates in 2014.
Alex understands the challenges of looking after your body whilst also leading a hectic and demanding lifestyle.
Her sessions aim to make Pilates accessible, relevant and most importantly - fun!
To book a Private 1:1 Session with Alex please call The Body Control Pilates Centre on 020 7636 8900.


text:
Analytical Cellular Pathology
Analytical Cellular Pathology/
AbstractIntroductionMaterials and MethodsResultsDiscussionData AvailabilityEthical ApprovalConsentConflicts of InterestAuthors' ContributionsAcknowledgmentsReferencesCopyrightRelated Articles
HPV E6/E7, hTERT, and Ki67 mRNA RT-qPCR Assay for Detecting High-Grade Cervical Lesion with Microscope Slides
Geehyuk Kim,1Jemberu Taye,2Kwangmin Yu,1Sunyoung Park,1Jungho Kim,1Sunghyun Kim,3Dongsup Lee,4Hye-young Wang,5Kwang Hwa Park,2and Hyeyoung Lee1
Academic Editor: Madhyastha Harishkumar
Published14 Jan 2019
After breast and colon cancer, cervical cancer is the third most common cancer of women worldwide. Since human papillomavirus (HPV) infection is known to be the predominant cause of cervical cancer, molecular HPV screening is currently used along with cytological and histological examination methods for precancer diagnosis. Nevertheless, the sensitivity of the current HPV test is less than 80%; thus, many cervical cancer cases are not able to be diagnosed by HPV screening alone, and likewise, patients with cervical cancer are often determined to be HPV-negative by the current screening methods. Therefore, human telomerase reverse transcriptase (hTERT) and Ki67 previously identified as cancer markers were attempted. And cervical exfoliated cells of high-grade squamous intraepithelial lesion (HSIL), the most severe precancerous lesion of cancer, were used in the study. However, it takes a long time to collect enough specimens to conduct statistical analysis. Therefore, in the present study, microscope slides, cervical exfoliated cells on glass slides, were attempted. The results of the analysis demonstrated that hTERT and Ki67 expression levels were useful in distinguishing between cancerous and normal specimens, exhibiting a higher sensitivity and specificity than conventional HPV E6/E7 testing. And the study suggests clinical slide cell samples could be effectively used in the context of retrospective studies to identify novel biomarkers.
Cervical cancer is the third most common cancer of women worldwide, after breast and colon cancer [1]. The World Health Organization (WHO) estimates that approximately 528,000 women are diagnosed with cervical cancer each year and that the disease results in approximately 266,000 deaths annually.
Known risk factors for cervical cancer include human papillomavirus (HPV) infection, promiscuous intercourse, sexually transmitted disease infection, long-term hormonal contraceptive use, and smoking [2]. Of these risk factors, HPV infection is the predominant cause of cervical cancer. Thus, effective HPV screening is essential to facilitate accurate and rapid precancer diagnosis and is currently used along with cytological and histological examination worldwide [3–6].
Current "gold-standard" methods for precancer diagnosis are cytological and histological examinations. For cytological examination, exfoliated cervical cells are collected by swabbing the cervix (as part of a "Pap-smear" test), before being placed onto a slide to be inspected for abnormalities. In case of histological examination, it is diagnosed via a microscopic examination of a stained tissue biopsy [7]. Both of these precancer diagnosis methods are affected by sensitivity of test itself and examiners' subjectivity.
Recently, a molecular method via the identification of HPV nucleic-acid sequences was developed for use in conjunction with standard cytological and histological examination techniques, commonly [8].
Currently, commonly used diagnostic markers include the HPV-related proteins L1, E6, and E7. Of these, L1 is a major viral capsid protein that is produced in the cytoplasm, before being translocated into the nucleus of intermediate and superficial squamous epithelial cells, as previously visualized using immunochemical staining. E6 and E7 are primary HPV oncoproteins with numerous cellular targets including p53, and the retinoblastoma tumor suppression protein (pRB). E6 inhibits p53 to prevent apoptosis, whereas E7 is the primary transforming protein, and inhibits pRB to regulate cell-cycle arrest [9, 10]. In the previous study, we assessed the efficacy of cervical cancer diagnosis via screening for the mRNA expression of commonly used HPV markers L1, E6, and E7, along with the additional cancer markers human telomerase reverse transcriptase (hTERT) and Ki67. hTERT represents the catalytic subunit of telomerase. Telomeres are highly specialized structures that are located at chromosome ends and are known to be essential for genome stability [11]. In fact, telomere dysfunction and telomerase activation have been previously implicated in human cancer progression [12]. The expression level of hTERT is known to be the rate-limiting factor for human telomerase activity, and as such, likely a more sensitive indicator of telomerase function and activity than the expression levels of other telomerase subunits that are constitutively expressed in both normal and cancer cells [13]. On the other hand, Ki67 is a nuclear antigen expressed during all active phases of the cell cycle (i.e., G1, S, G2, and M) except G0, and thus, its expression level can be used to determine the cell proliferation status and to predict tumor development [14].
Screening of these diagnostic markers may also be of use in assessing the progression of cervical cancer past the midstage, as demonstrated by a previously conducted prospective study of their expression in formalin-fixed paraffin-embedded (FFPE) clinical histological samples [15]. Cytological samples actually used in clinical screening test were also conducted. However, severe precancerous lesion samples were not enough to conduct statistical analysis. Especially it takes a long time to collect high-grade squamous intraepithelial lesion (HSIL) samples which are the most severe precancerous lesion of cancer. Therefore, in the present study, microscope slides were attempted as samples. They are sealed with Canada balsam in a vacuum state which induce longer storage period relatively. And they could be collected quickly and easily through documented clinical information.
In the present study, HPV and cancer markers mentioned above were analyzed with 110 HSIL and 50 normal microscope slides.
2.1. Clinical Samples
A total of 110 and 50 slides with exfoliated cervical-cell samples mounted with Canada balsam (Merck, Frankfurter, Germany) were retrospectively obtained from patients diagnosed to HSIL and normal, respectively, between 2000 and 2004, from the Department of Pathology, Yonsei University Wonju Severance Christian Hospital, Wonju, Republic of Korea. Strictly speaking, normal means negative for intraepithelial lesions or malignancy (NILM) in this context. To reduce interpretive diagnostic error, we only utilized HSIL specimen confirmed with cervical intraepithelial neoplasia grade 2 or worse (CIN2+).
All subjects provided written informed consent for their participation in the study, which was approved by the Institutional Ethics Committee at Yonsei University Wonju College of Medicine (approval no. CR315052).
2.2. Histological and Cytological Diagnosis
Clinical diagnosis was predominantly determined cytologically using the 2001 Bethesda System terminology; however, cases with available tissue biopsies were also histologically reviewed.
2.3. Slide Preparation and Total RNA Extraction
Slides with exfoliated cervical cells (microscope slides) were used for total RNA extraction. Slides were initially placed in coplin jars with xylene (Duksan, Ansan, Republic of Korea) for 4 days to remove their cover clips (which were mounted with Canada balsam). They were then dried (5 min) and placed into a six-well culture plate (SPL Life Sciences Co., Pocheon, Republic of Korea). The Isol-RNA Lysis Reagent (1 mL; 5 Prime, Austin, TX) was added onto each slide, and a 1 mL exfoliated cervical-cell sample was then collected from each slide via scraping (twice) with an autoclaved slide glass. Each collected exfoliated cervical-cell sample was transferred to an RNase-free 1.7 mL tube, lysed via vortexing/repeated pipetting, and allowed to incubate in the reagent (room temperature, 5 min). After the addition of 200 μL of chloroform, the tube was shaken vigorously, incubated (room temperature, 3 min), and then centrifuged (12,000 g, 15 min). The resultant aqueous layer was transferred to a new tube and mixed with an equal volume of isopropanol by inverting the tube. The mixture was incubated (25°C, 10 min) and then centrifuged (12,000 g, 10 min) before the resulting supernatant was removed, and 1 mL of 75% ethanol was added to the remaining pellet. After mixing via tube inversion, the mixture was centrifuged (7,500 g, 5 min), and the supernatant subsequently removed. The remaining RNA pellet was dried and eluted in 25 μL of diethylpyrocarbonate- (DEPC-) treated water (Intron Biotechnology, Seoul, Republic of Korea). The purity and concentration of the extracted total RNA was determined by measuring its absorbance at 260 and 280 nm using an Infinite 200 plate reader (Tecan, Salzburg, Austria). The isolated total RNA was finally stored at −70°C until use. Note that all preparation and handling of total RNA were performed in a laminar flow hood, under RNase-free conditions.
2.4. cDNA Synthesis
Complementary DNA (cDNA) was synthesized using an M-MLV Reverse Transcriptase kit (Invitrogen, Carlsbad, CA, USA) and random hexamers (Invitrogen), according to the manufacturer's recommendations. Briefly, 10 μL of total RNA was added to a master mix containing 1 μL of 10 mM dNTP mix (containing 10 mM each of dATP, dGTP, dCTP, and dTTP at a neutral pH), 0.25 μg of random hexamers, and 1 μL of DEPC-treated water in PCR tubes. The reaction mixture was incubated (65°C, 5 min) and then quickly chilled on ice. A mixture of 4 μL of 5× First-Strand Buffer, 2 μL of 0.1 M dithiothreitol (DTT), and 1 μL of M-MLV reverse transcriptase (RT) was added to the reaction mixture, and the cDNA synthesis reaction then performed via cycling at 25°C for 10 min, 37°C for 50 min, and 70°C for 15 min.
2.5. HPV Genotyping Using PCR-REBA
A REBA HPV-ID® PCR-REBA test (YD Diagnostic, Yongin, Republic of Korea), in which a "nested PCR" method was used to amplify target regions between MY11-MY9 and GP5-GP6 using two primer pairs, was used for HPV genotyping. PCR was performed using a 20 μL reaction mixture (Genetbio, Daejeon, Republic of Korea) consisting of 2× master mix, 1× primer mixture, 3 μL of sample DNA, and sterile deionized water (DW). This mixture was subjected to PCR cycling conditions comprising 94°C for 5 min (predenaturation), followed by 15 cycles of 94°C for 30 s (denaturation) and 55°C for 30 s (annealing), 45 cycles of 94°C for 30 s (denaturation) and 52°C for 30 s (annealing), and a final cycle of 72°C for 7 min (strand synthesis). The amplified biotin-labeled PCR products were then denatured (25°C, 5 min) in denaturation solution, diluted in 970 μL of hybridization solution, applied to the REBA membrane strip in the blotting tray, and hybridized (50°C, 30 min) to the desired probes. The membrane strips were then washed twice with 1 mL of washing solution (50°C, 10 min, with gentle shaking), before being incubated (25°C, 30 min) with a streptavidin-alkaline phosphatase (AP) conjugate (Roche Diagnostics, Mannheim, Germany) diluted (1 : 2 000) in a conjugate diluent solution (CDS). After two final washes with 1 mL CDS (room temperature, 1 min), colorimetric hybridization signals were visualized via incubation with an NBT/BCIP solution (1 : 50 dilution, Roche Diagnostics) for sufficient time to detect the enzymatic conversion of the NBT/BCIP substrate to its colored form. The resulting band patterns were then read and interpreted.
2.6. Multiplex Quantitative Reverse-Transcriptase (RT-Q) PCR Assay
HPV E6/E7, hTERT, and Ki67 mRNA expression in cervical specimens was assessed via a multiplex RT-qPCR TaqMan assay that was performed using the CervicGen HPV E6/E7 and hTERT-Ki67 mRNA RT-qDx assay kits (Optipharm, Osong, Republic of Korea) and the CFX-96 real-time PCR system (Bio-Rad, Hercules, CA, USA) for thermal cycling and fluorescence detection. Real-time PCR amplification of HPV E6/E7 mRNA was performed in a reaction mix containing 10 μL of 2× Thunderbird probe qPCR mix (Toyobo, Osaka, Japan), 5 μL each of primer and TaqMan probe mixture, and 5 μL of template cDNA. Real-time PCR amplification of hTERT and Ki67 mRNA was performed in a reaction mix containing 10 μL of 2× Thunderbird probe qPCR mix (Toyobo, Osaka, Japan), 3 μL of primer and TaqMan probe mixture, 2 μL of template cDNA, and sufficient DW to produce a final volume of 20 μL. Positive and negative controls were included throughout the procedure, and likewise, no-template controls (containing sterile DW instead of template DNA) were amplified with each PCR. The utilized PCR cycling conditions comprised 95°C for 3 min, followed by 41 cycles of 95°C for 3 s, and 55°C for 30 s. Each mRNA expression level was quantified by determining the "cycle threshold" (CT), which is the number of PCR cycles required for the fluorescence to exceed a value significantly higher than the background fluorescence. To avoid the generation of false negative results due to mRNA degradation, the expression level of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal control. The six samples of HSIL group and four samples of normal group did not show GAPDH value; therefore, they were excluded from the following experiments. In other words, researcher performed experiments with 104 HSIL and 46 normal samples. Target gene mRNA expression levels relative to GAPDH were automatically calculated according to the comparative Ct method, using CFX Manager v1.6 (Bio-Rad) or Genex (Bio-Rad) Software. Gene expression was assessed using the comparative Ct (ΔΔCt) method, in which mRNA expression levels are represented relative to the expression level of the reference gene. hTERT and Ki67 expression levels in analyzed slide samples from patients without HSIL were considered to indicate the "baseline" expression level for each gene.
2.7. Statistical Analyses
Statistical analyses were performed using GraphPad Prism software version 5.02 (GraphPad, La Jolla, CA, USA). Student's t-test, 95% confidence interval (CI), and ROC curve were used to assess the statistical significance of generated data. Cohen's kappa coefficient which measures agreement between two raters for qualitative items is also used.
3.1. Histological and Cytological Diagnosis of Clinical Specimens
All analyzed slide samples were confirmed to exhibit the precancerous condition, cervical intraepithelial neoplasia (CIN) III. Furthermore, histological and cytological methods were used to confirm a diagnosis of HSIL among the 110 relevant slides. Patients with HSIL (range 10-79 years-of-age) were found to be predominantly aged between 30 and 39 (36/104 patients, 34.62%) or 40 and 49 (35/110 patients, 33.65%) years. A lesser number of HSIL patients were aged between 20 and 29 (11/104 patients, 10.58%) or 50 and 59 (12/104 patients, 11.54%) years, and very few were aged less than 20 or greater than 59 years (Table 1).
Cytological diagnosis of clinical specimens with respect to patient age.
3.2. REBA Analysis of the HPV Infection Status of Analyzed HSIL Clinical Specimens
Of the 104 slides with exfoliated HSIL cervical-cell samples, 83 (79.81%) were found to be infected with at least a single HPV genotype, including 26 (25%) that were infected with multiple (i.e., more than two) HPV genotypes. Among these 83 cases, 56 (53.85%) were determined to be infected with a high-risk (HR) HPV genotype, while a single case (0.96%) was shown to be infected with a low-risk (LR) HPV genotype (Table 2). Among the 26 cases found to be infected with multiple HPV genotypes, 21 (20.19%) were shown to be infected with HR-HPV genotypes, five (4.81%) were determined to be infected with both HR- and LR-HPV genotypes, and no cases were found to be infected with LR-HPV genotypes only.
Distribution of analyzed HSIL specimens with respect to HPV infection type (as assessed via REBA).
3.3. HPV Genotype Distribution in the Analyzed Clinical Samples
As shown in the constructed cumulative graph (Figure 1), the most frequently detected HPV genotype in the HPV-positive exfoliated cervical-cell samples was HPV 16 (cumulative proportion 40.22%), followed by HPV 52 (55.43%), 58 (66.30%), 31 (77.17%), 18 (84.78%), 33 (90.22%), 35 (94.57%), 66 (97.83%), 84 (98.91%), and 45 (100%).
Cumulative graph of human papillomavirus (HPV) genotype distribution among the analyzed high-grade squamous intraepithelial lesion (HSIL) clinical specimens. To inspect HPV distribution, HPV-positive specimens were analyzed in descending order. It is shown as a cumulative graph to check the degree of occupation.
3.4. hTERT and Ki67 mRNA Expression Levels as Determined by RT-qPCR
We conducted RT-qPCR analyses of hTERT and Ki67 expression levels in the 104 HSIL-diagnosed exfoliated cervical-cell samples. After excluding samples with a no GAPDH expression level, we determined that 98 (94.23%) and 49 (47.12%) of the 104 remaining HSIL samples were positive for hTERT and Ki67 mRNA expression, respectively (Figure 2).
Analysis of the relative expression of hTERT (a) and Ki67 (b) in high-grade squamous intraepithelial lesion (HSIL) versus normal clinical samples using the delta Ct method. As shown in (a) and (b), normal and HSIL groups were distinguished by hTERT and Ki67 gene expression showing a statistically significant difference (), respectively. The cut-off value for distinguishing between positive and negative results is determined from the receiver operating characteristic (ROC) curve.
3.5. Coincident Expression of hTERT or Ki67 with HPV E6/E7 mRNA in the Analyzed HSIL Clinical Samples
The results of the conducted RT-qPCR analyses showed that of the 104 (nonexcluded) HSIL samples, 80 (76.92%) were positive for both HPV E6/E7 and hTERT, three (2.88%) were positive for HPV E6/E7 only, 18 (17.31%) were positive for hTERT only, and three (2.88%) were positive for HPV E6/E7, but negative for hTERT (Table 3). Conversely, 38 (36.54%) of the 104 samples were shown to be positive for both HPV E6/E7 and Ki67 expression, 45 (43.27%) were positive for HPV E6/E7 expression only, 11 (10.58%) were positive for Ki67 expression only, and ten (9.62%) were positive for HPV E6/E7, but negative for hTERT expression (Table 4).
RT-qPCR analysis of HPV E6/E7 and hTERT mRNA expression in HSIL versus normal clinical specimens.
RT-qPCR analysis of HPV E6/E7 and Ki67 mRNA expression in HSIL versus normal clinical specimens.
3.6. Combination of HPV E6/E7, hTERT, and Ki67 mRNA Expression
The positivity rates were 90.38% (94/104) for a combination of HPV E6/E7 and Ki67 mRNA expressions, 96.15% (100/104) for hTERT and Ki67 mRNA expression, 97.12 (101/104) for HPV E6/E7 and hTERT mRNA expressions, and 98.08% (102/104) for the HPV E6/E7, hTERT, and Ki67 mRNA expressions (Figure 3).
Combined expression patterns of HPV E6/E7, hTERT, and Ki67 mRNA in high-grade squamous intraepithelial lesion (HSIL) clinical samples. All of HSIL group could not identify with one marker; therefore, combinational detection of multiple target was tried. Combination of HPV E6/E7, hTERT, and Ki67 showed 98.08% (102/104) positive.
The previous study was conducted with FFPE clinical tissue samples [16]. It was appropriate to determine the availability of the biomarker by tissue samples because tissue samples collected precisely only cancerous region through microscopy and IHC test. We also confirmed that hTERT and Ki67 mRNA expression could be complementary biomarkers in diagnosing cervical lesions with histological samples. In the present study, microscope slides with exfoliated cervical cell samples were collected from subjects diagnosed as either healthy or with HSIL and screened for HPV genotypes, assessing the usefulness of hTERT and Ki67 expression as diagnostic markers of cervical cancer. Exfoliated cervical cells before being placed onto a slide are currently used for screening test specimen because they accompanied with less invasive and less labor-intensive procedure than other tests [17, 18]. They are exposed to the air and need refrigeration condition, so their storage period is limited. Above all, precancerous lesion samples are relatively infrequent compared to normal samples, and there is no idea how much time needed to collect enough samples for statistical analysis. In fact, HSIL samples, which are the most severe precancerous lesion, were collected over three years in Korea and China, but there were about 50 samples [16, 19]. In contrast, microscope slides are sealed with Canada balsam in a vacuum state, so their storage period is longer relatively. In fact, there was no difference in the housekeeping gene expression between samples by 5 years. And the slides were always prepared for cytology test as routine screening test. These factors enable to collect large number of HSIL samples over 100 easily and immediately. Thus, the slides are adequate specimen for retrospective cohort study. There were several retrospective studies with the slides; however, they are primarily limited to inspect staining of appearance so far [20–23]. For the first time, molecular tests were performed with microscope slides in this study. To verify nucleic acid degradation and variation, the assays were performed with endogenous control genes during the experiment.
The results showed that HR-HPV infection is more closely associated with cervical cancer progression than LR-HPV both in the context of a single or of multiple HPV infections (Table 1). In addition, the most commonly detected HPV genotype among the HPV-positive HSIL specimens was HPV 16, and notably, detection rate of HPV 18 of slides is lower than that of histological samples.
Cervical cancer oncogenesis is initiated and mediated via the upregulation of the HPV oncoproteins E6 and E7, such that the overexpression of E6/E7 mRNA transcripts has been shown to be associated with a significantly increased risk of both precancerous group (CIN) and of cervical cancer [24]. The hypothesis that E6/E7 expression levels may be specific and effective predictors of cervical cancer risk was supported by the results of the present study, which showed the sensitivity of the utilized E6/E7 mRNA RT-qPCR assay to 79.81% in the 104 analyzed exfoliated cervical-cell samples. It indicate cervical cancer occurrence could be affected other factors not only HPV. Therefore, hTERT and Ki67 confirmed by tissue samples [16] were also applied. The sensitivity of hTERT mRNA RT-qPCR screening of the 104 clinical samples was 94.23%, whereas that of Ki67 screening was only 47.12%. Interestingly, a previous study demonstrated hTERT mRNA expression to be higher in cytological than in tissue samples from high-grade cervical lesions, while conversely, Ki67 mRNA expression was found to be higher in tissue than cytological samples from the high-grade cervical lesions [20]. The mechanism underlying this observed discrepancy between marker genes expression in cytological versus histological samples remains to be elucidated.
While hTERT and Ki67 mRNA expression was only detected in 94.23% and 47.12% of the analyzed cytology samples, respectively, combined screening for HPV E6/E7 and Ki67, HPV hTERT and Ki67, and HPV E6/E7 and hTERT mRNA expression identified 90.38%, 96.15%, and 97.12% of samples, respectively (Figure 3). Furthermore, coincident screening for HPV E6/E7, hTERT, and Ki67 mRNA expression resulted in an RT-qPCR assay sensitivity of 98.08%, suggesting this as a promising combination of markers for the diagnosis of HSIL.
The present study demonstrates the validity of using the non-HPV markers and of analyzing microscope slides for the first time to identify novel diagnostic precancer and cancer biomarkers. Further study is required to assess the suitability of their use as diagnostic markers for low-grade squamous intraepithelial lesions (LSIL) and/or as indicators of the progression of cervical lesions.
The datasets generated and analyzed during the current study are not publicly available due to patent application but are available from the corresponding author on reasonable request.
This is to state that I give full permission for the publication.
The authors declare that they have no competing interests, and all authors should confirm its accuracy.
Jemberu Taye collected the patient clinical data and specimens. Kwangmin Yu performed the experiment. Geehyuk Kim analyzed and interpreted the patient data. All authors read and approved the final manuscript.
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT, and Future Planning (2015R1A2A2A04004455). This work was also supported (in part) by the Yonsei University Research Fund of 2018.
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H. Y. Wang, G. Kim, H. Cho et al., "Diagnostic performance of HPV E6/E7, hTERT, and Ki67 mRNA RT-qPCR assays on formalin-fixed paraffin-embedded cervical tissue specimens from women with cervical cancer," Experimental and Molecular Pathology, vol. 98, no. 3, pp. 510–516, 2015.
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Copyright © 2019 Geehyuk Kim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The Wesleyan Outing Club is a student run group, started in 1937. The Club's headquarters are located at Outhouse, 132 High St. The Club offers a wide range of trips most weekends such as biking, hiking, canoeing, and rock climbing, and longer trips over breaks. Past trips have included winter camping in the White Mountains, biking to nearby Miller's Pond, and hiking through New England forests near and far. Anyone can join us—absolutely no experience is necessary for most trips. You can be as involved as you please—come on one outing a year, or take on a leadership position. To hear about Outing Club events, sign up for out weekly listserv, now through orgsync. We also have a supply of outdoor gear that you can rent for your own trips including sleeping bags, tents, stoves, canoes, kayaks, and cross country skis.
Learn all about our 2015 Freshman Orientation Trips!
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text:
800000 euro gift for tiny French town in Calvados
6.58 km2 (2.54 sq mi)
Population 312 (2008)
A small town in Lower Normandy named Jort (Calvados, 300 inhabitants) has just had its budget nearly quadrupled as a result of a bequest of 800,000 Euros, from a native of the town who died in December at age 86, officials said.
The benefactor who wanted to leave much of his fortune to the town was previously little known to its inhabitants. Based in Annecy, he would return to Jort once or twice a year although he had no home there to see the grave of his parents.
The benefactor "Pierre Auger" hoped that this money is used for social purposes.
Mr. Auger was heir to a childless old brother, with whom the mayor intends however to contact, and who has inherited land they owned in undivided, and a godson whom he was close and "must be part of a life insurance policy."
Jort is a small town known for its raw milk camembert cheese. Its last cencus revealed a population of 312 covering a 2.54 square mile area.
The legacy consists of the sale of two apartments that Mr. Auger had in Annecy and Vincennes (valued at 650,000 euros), with the remainder in cash and shares.
It will likely take several years to dispose of some 800,000 Euros. After investing one million euros in the off-mains drainage the 2010 budget for Jort was 230,000 euros.
"If it could help us find a doctor. Ours has just left town and those that are here are overwhelmed,"
said Mayor Jean-François Guillemot.
History of Dom Perignon champagne
The big world
Posted by asking questions at Tuesday, February 22, 2011
What is a Dobro?
What is a Dobro or Resonator slide guitar? The Dobro or Resonator guitar is a guitar familiar to many as the lap steel sounding instrume...


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text:
Q: Returning directly to main from a function call of a function call just as a disclaimer this question has to do with a particular assignment I have an I am not asking for anyone to do my homework for me.
So basically I am supposed to implement a way to return directly to main from a function call of a function call.
Part of the stipulations are that we cannot use an assembly language instructions, gcc's asm(), or gcc built ins. After doing a lot of research on this on google I couldn't really find any examples to look at or even the source code for setjmp/longjmp (the purpose of this assignment is to copy those functionalities). I've asked some more older CS students for advice and most couldn't help or told me they are pretty sure it is not possible with the stipulations given. Any advice or pointers (haha) will be appreciated. Even a nudge in the right direction or confirmation that the assignment is not as complicated as I think will be greatly appreciated!
So far my best attempt:
-Use a setjmp function to store the address of where we left off in main (so something like x = foo1(); and pass x into setjmp(x)) and then have foo2 call my longjmp function where in longjmp I'll have the function set a pointer (*p) to my argument and then (*p-1) = address of x in main.
This didn't work but I thought it was the right idea trying change the return address in the call stack since if I understood it correctly, the arguments of the function are directly on top of the return address in the stack.
Here is the code I wrote:
int setjmp(int v);
int longjmp(int v);
int fun1(void);
int fun2(void);
int *add; //using global, not sure if best idea
int main(void)
{
    int x = setjmp(x);
    foo1();
    return 0;
}
int setjmp(int v)
{
  add = &v; //used a global variable
  return 0;
}
int longjmp(int v)
{
  int *p; //pointer
  p = &v; //save argument address
  *(p-1) = *add; //return address = address in main
  return 1;
}
int foo1(void)
{
    printf("hi1");
    foo2();
    printf("hi2");
    return 0;
}
int foo2(void)
{
    int a;
    longjmp(a);
    return 0;
}//output SHOULD be "hi1"
 //output is currently "hi1" "hi2"

for what it's worth every line I have not commented was given as a skeleton and I cannot change it.
Excuse me in advance if something is off, I am quite new to C. Thank you.

A:
"So basically I am supposed to implement a way to return directly to main from a function call of a function call. "

This requirement is nonsense. Any attempt to sate that requirement will result in trash code. Training to write things like this is directly harmful practice. This is a very bad assignment and your teacher should be ashamed for teaching you bad practice with no disclaimer. There is also never a reason to do things like this in real-world programs.
You can't implement the setjmp/longjmp functions in pure C, you would have to use inline assembler. They save the program counter and other such things needed by the specific system. They also meddle with the stack pointer, which is one reason they are dangerous.
So the only way to do this in standard C is to use the standard library functions setjmp/longjmp from setjmp.h. These are widely considered very bad and dangerous since they lead to unreadable spaghetti programming and many forms of undefined behavior. One example of undefined behavior from the C standard:

After a longjmp, there is an attempt to access the value of an object of automatic
  storage duration that does not have volatile-qualified type, local to the function
  containing the invocation of the corresponding setjmp macro, that was changed
  between the setjmp invocation and longjmp call

Don't use these functions ever.

That being said, this is how you write horrible, dangerous programs:
// BAD! NEVER WRITE SPAGHETTI CODE LIKE THIS!

#include <stdio.h>
#include <setjmp.h>
#include <stdbool.h>

static jmp_buf jmp_main;

void func2 (bool one_more_time)
{
  puts(__func__);
  if(one_more_time)
  {
    longjmp(jmp_main, !one_more_time);
  }
  printf("end of "); puts(__func__);
}

void func1 (bool one_more_time)
{
  puts(__func__);
  func2(one_more_time);
  printf("end of "); puts(__func__);
}

int main (void)
{
  bool one_more_time = (bool)!setjmp(jmp_main);
  puts(__func__);
  func1(one_more_time);
  printf("end of "); puts(__func__);
}

Output:
main
func1
func2
main
func1
func2
end of func2
end of func1
end of main

A: I don't think problem is that complicated. You should be calling another function with a condition on returning value such that if it is certain value (say 0) then return otherwise continue on the function.
So you can just replace foo2(); with :
if (foo2() == 0) return 0;

Now in foo2() function return 0; whenever you wish to return to main function call of foo1() otherwise continue with foo1() statements. You need not use setjmp here.
You may also find this article helpful: Tread programming examples

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text:
Performing arts careers come to life
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See Greenwich Village, SoHo and Rockefeller Center.
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There's no substitute for hands-on experience. Immerse yourself in every aspect of the performing arts, from makeup demonstrations to acting workshops.
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