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Like so many other publications, the New York Times recently published a roundup of the "best songs" of the year. Reading through it, I was instantly struck by how many of the songs and artists I had never heard of before. Sure, Beyoncé, Taylor Swift, and the 1975 all made appearances — I knew all of them, of course, even if I'd only ever heard the 1975's song — but most of the others I was learning about for the very first time.
Similarly, I only knew three of the songs on Paste's list of the 50 best songs of 2022.
It's not that I don't listen to a lot of music. According to Spotify Wrapped, I listened to 8,314 minutes of it this year — more than 54% of other listeners in the United States, apparently. And that's not including all the music I listened to on the radio, or that I'd purchased or downloaded, or that I heard live, or that I streamed via YouTube or other services. When I wasn't listening to podcasts like Smartless, that is.
And it's not that I don't listen to popular artists. Heck, my top artist this year (again, according to Spotify) was Harry Styles.
The thing is, music, more than most any other entertainment, is personal. So, how we classify the "best" or "top" songs, albums, or artists varies. My favorites are likely not your favorites. And I'm more than okay with that.
Which is why, every year at this time, I don't even bother to compile a year-end best-of list. Nor do I put much stock into Spotify's roundup of what my most listened-to tracks were — though, admittedly, the algorithm doesn't lie; I did listen to a lot of Harry Styles this year.
Rather, I prefer to review my annual "time capsule" playlist and let that define my year in music. Conveniently, the playlist also provides a soundtrack of memories for how the year played out (pun intended).
Categories concerts, music, year-end favorites
My 2021 Soundtrack Reflects the Times (and Music) I Want to Remember
When directors make movies, they work with a music supervisor and a composer to create a soundscape for the film that will deliberately help drive the narrative, boost the desired emotional impact, and leave a distinct impression in the minds of viewers. When done well, hearing a particular song or musical motif included in the movie may trigger memories of that work.
In real life, the process happens somewhat in reverse: The "narrative" of our lives moves forward organically, and the music that triggers memories of certain times and events is not necessarily planned. And, rather than the music itself telling a story, it's up to us to look at the collection of songs and pick out the themes from the soundtrack in hindsight. In this way, music serves to remind us of the times we've lived through, and the music that was playing while we lived — with this caveat: The soundtrack often reflects the times we want to remember. More importantly, it's made up of the music we want to remember.
I listened to a lot of music in 2021, and my "Now" playlist was everchanging. But as noted, the soundtrack of my year — a.k.a. my 2021 Time Capsule Playlist — largely reflects what was good about these last 12 months.
Categories Broadway, Bruce Springsteen, concerts, looking back, movies, music, playlist, stuff I did, TV, year-end favorites
When the Dust Settled, These Are the Songs That Got Me Through 2020
In times of stress, as 2020 most certainly was, music always seems to serve as a comfort. And over the last nine and a half months, while some other forms of entertainment and culture had to be put on hold for safety reasons, there was never a shortage of music to listen to and seek comfort from.
Sure, we couldn't crowd together for concerts, or see other kinds of live performances in person — such as Broadway shows and other theatrical productions. And that definitely sucked. As Dave Grohl wrote all the way back in May, "I'm hungry for a big old plate of sweaty, ear-shredding, live rock and roll, ASAP. The kind that makes your heart race, your body move, and your soul stir with passion." Yeah, me too.
But on the other hand, this year, the artists came to us, hosting intimate live streams on Instagram, Facebook Live, Zoom, or other platforms; performing from their homes on various TV or online specials; sharing archival performances on YouTube; and more. It wasn't what we hoped for back in January, and there were some kinks to work out at first. But in the end, it was actually kind of cool.
Categories concerts, music, playlist, year-end favorites
I'm Putting These Songs in My 2019 Time Capsule to Preserve Good Memories
I love Spotify. As someone who listens to a lot of music, Spotify Premium is one of the things I'm most thankful for, because it allows me to have a seemingly bottomless collection of tracks and albums at a minimal price, and it exposes me to songs and artists I might not hear otherwise because I don't listen to a lot of radio.
Every year, Spotify does its "Wrapped" marketing campaign, where it shares with users — in easily shareable form, natch — their most-listened-to artists and tracks. It's hard to argue with the results, since, apparently, they're based on Spotify's own data. And yet, every year, I have a hard time reconciling Spotify's results with the music I think actually represents my year.
And that's why, for the past few years, I've compiled a running "time capsule" playlist, curating the songs and artists I was listening to at various points in the year, or ones that represent significant or memorable things I experienced. I like recapping my year in music this way instead of waiting and compiling a year-end top-music list, because rather than focusing on a few favorite albums or songs, with this playlist, I have a better representation of what was literally going through my head all year long. Continue reading →
My Soundtrack for 2018 Brings Back a Lot of Memories
Every year has its own soundtrack. It's the collection of songs and sounds that, when you hear them, they remind you of times gone by.
My soundtrack for 2018 does exactly that, recalling the times I spent lazing by the pool aboard a cruise ship, watching the Olympics, celebrating my birthday in New York City, enjoying live music, driving around in the summer with the volume up and my car windows down, and much more.
I like compiling this kind of "time capsule" playlist instead of writing a top-10 (or whatever number) list of the year's "best" songs and albums because music tends to play such a large role in my life, and I often can remember certain events by what I was listening to at the time. Besides, taste is so subjective, so when looking back on the year gone by, I'd much rather call attention to more music than less. Because tracks wouldn't have been added to this playlist if I didn't like them, anyway. Continue reading →
Categories Broadway, Bruce Springsteen, concerts, looking back, music, stuff I did, year-end favorites
The 2018 Happiness Project
I don't know much, but I know this: You can't plan to be happy.
That said, you can make an effort to be mindful and appreciate good things that happen and that make you smile. And those things will make you happier. It's a simple idea, but it's an effective one.
And being happy has many benefits — not least of which is that nobody wants to hang around with a perpetual Debbie Downer. But all kidding aside, being happy has multiple health benefits, including increased strength and decreased stress, a stronger immune system, and reduced risk of coronary heart disease. Scientific evidence also suggests that happiness can help you unlock creativity, improve your memory, and make you more productive. So, it's really not a trite or superficial (or sexist) thing when someone wishes you a "Happy New Year" or "Happy Birthday" or "Happy Holiday," or suggests you smile more. They're actually wishing you increased wellness and productivity. Continue reading →
Categories concerts, family, Happiness Project, looking back, music, self-improvement, stuff I did
This Time Capsule Playlist Provides a Musical Reminder of All the Moments that Mattered in 2017
The Lone Bellow at the House of Blues in Boston, November 2017. (Photo credit: Martin Lieberman)
Every December, critics (both professional and amateur) like to share their lists of the year's best music. And while that's important, and much appreciated, it's near impossible for music fans to agree on what's "best." Musical taste is too personal. Case in point: For every Taylor Swift fan out there (and I know there are a lot of them), there's someone like me who can't stand her. On the other hand, I have a soft spot for other artists who don't often show up on top 10 lists of best music and don't win many awards. John Mayer, for example.
Point is: We all listen to different things and like what we like. And that's a great thing.
So, when the end of the year comes around, I choose a different angle on the music recap thing, and prefer to compile a sort of "time capsule" playlist that provides a soundtrack of my year. Continue reading →
Categories concerts, looking back, music, stuff I did, year-end favorites
Can I Interest You in Hanukkah Songs … that Aren't Sung by Adam Sandler?
Anyone who knows me knows there are few things I love more than Christmas music. I made Very Marty Xmas mixes for 10 years, and continue to maintain an "Ultimate" playlist version of the mix on Spotify that includes everyone from Mariah Carey to Andy Williams to Harry Connick, Jr. to Darlene Love singing festive tunes, and is updated all the time to include new tracks.
But here's the rub: I'm Jewish. And one thing that bothers me every year is that, despite how much I enjoy the Christmas season and all the music, I wish Hanukkah was better represented. Unfortunately, while Jews have written many of the most beloved Christmas songs (it's true!), we haven't done a very good job of writing songs for our own holiday. So those Members of the Tribe who are looking for seasonal music are often stuck with Adam Sandler's "Chanukah Song" — which was funny the first few times, but now, 13 years later, it's just tired (not to mention totally outdated). Continue reading →
Categories Christmas, Hanukkah, music, Very Marty Xmas
Is This the Most "American" July 4th Playlist of All Time?
Photo credit: Stephanie McCabe / Unsplash
You don't need a reason to make a playlist, but a holiday sure does provide a good one.
And today, on July 4th, one of my favorite days of the year, I like listening to one of my favorite Spotify playlists. Like my summertime mix, it's a collection of songs all built around a theme — in this case, the word "America." All included songs feature some use of the word America (or a variation of it) in a prominent way — which is why John Cougar Mellencamp's "Pink Houses" qualifies, but, say, "Horse with No Name," by the band America, does not. Bruce Springsteen's "4th of July, Asbury Park (Sandy)" may be an exception to that rule, but come on. How could I not include it?
And, because it's a mix for the Fourth of July, there are a few patriotic or July 4th or "Independence Day"–themed or related songs (like Chicago's "Saturday in the Park" and Katy Perry's "Firework," natch) thrown in too. Yes, there are even some songs, like Springsteen's "Born in the U.S.A.," that sound patriotic, but really aren't.
I call this playlist, no surprise, American Tunes. Continue reading →
Categories July 4, music, playlist
Something I've learned over the years is that happiness is not something you can seek or plan. You just have to allow yourself to feel it. To notice and appreciate the good things in life — no matter how small — that make you smile. Hopefully on a regular basis.
With so much negativity in the world, that can sometimes be hard. And this year, it may prove extra challenging.
So, to proactively prevent another sucky year like 2016 was, I want to do whatever I can to focus on my own happiness — and hopefully, in the process, share that happiness with others. Continue reading →
Categories concerts, Happiness Project, how much I ate, looking back, movies, music, self-improvement, stuff I did, TV

redpajama_set_name: RedPajamaCommonCrawl


Well it is done. Joie is now Mrs. acesn8s. Other than the best man showing up late with the tuxes (which in turn made us both late for our own wedding) all went well. Although, I was a little concerned when her brother paid to dance with me during the dollar dance. Good times were had by all and the inlaws and outlaws got along quite well.
Sorry it took so long to announce but she had me busy during the honeymoon.


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https://abcwednesday-mrsnesbitt.blogspot.co.nz/
For years, the resident of this premises had many Triumph cars parked not only at his car park, his neighbour's car park, but on the roads on both sides of his residence. They were an eye sore. He was dodgy when people asked why his cars were parked there, using up parking spaces. He wasn't tinkling with them. They were just parked there. He didn't seem to be collecting them as a hobby. Was he illegally selling them?
One day, those cars on the main road were removed. There are still other cars.
In the early 60s, we lived in the Government Quarters along with Malays, other Chinese dialect groups, Ibans, Eurasian and so on.
Near to the houses were a small Hindu temple . There were no windows but had louvers to admit light and air. We climbed up on the wall, my brother supporting me, and we saw some statues in gold. It was full of mystique. It looked deserted because there was nobody there.
In the garden was a deep walled in well. Some one rumored that some one had drowned in the well. The water was murky. We climbed to steal the sour lime like calamansi which we ate there and then. Then there were the gardenia flowers and buds. We stole them. The plants were high up and so we had to climb. Someone screams ghost and we went screaming home.
You see. Mum had warned us not to wander there.
For nostalgia sake, I went three years ago. The temple was gone, and the modern building replaced it.
My friend said it was the smallest temple in Malaysia.
grandma's slave
There is on internet a story on "My family's slave" by an Filipino American.
Here's an abstract of my grandma's slave from my book, From China to Borneo to Beyond.
The pronunciation of my Quang Ning dialect MUI ZUI, for a slave sounds like the sour plum, and MUI ZAI as a girl is different. I don't know what it is in other dialects.
It must have been 1900s when my grandmother brought her over to be her slave. The girl was very young. It is not sure if she grew up with Grandma, or given to her when she was married.
My father, John remembered fondly of Grandmother's mui zai (slave) whom he called Ah Jia, (big sister.) In fact he saw her more than he saw Grandmother. Grandmother worked in the rubber garden, the mui zai took care of him and his siblings. She did all the housework. She kindly separated the rough green husk of the sweet mung bean soup, so he would have it as a smooth watery thick soup.
There was talk that the British government in Malaya and Singapore was going to pass an emancipation of slaves, and those not releasing the slaves would be punished.
To preempt this, when this mui zai was 16, a marriageable age, Grandfather Kee Seng arranged for a suitable mate and married her off. This was much to the aghast of Grandmother. Grandmother whinged that this mui zai was paid for by her parents; therefore she was her property. This mui zai was her slave for life. Grandfather Chan had no right to sell her property. But Grandfather would not have any part of this old feudal slavery system. They married her off to someone up the Rejang River.
The emancipation law was never passed and Grandfather never heard the end of Grandmother harping on and on about it.
Some of those mui zais maintained a good relationship, coming back to the family as though they were part of the family. In many cases where they had suffered abuse from their owner and hated them; they never came back to visit. Grandmother's mui zai never came back. Some, their new family forbidden them to.
Father did meet the mui zai many years later. Father was on official duty in a school near where she was married off to. She came and was hesitant to talk to Father, now an official of the government. She wanted Father to help her grand children to get into teachers' college. She said quietly that it wasn't that she didn't want to visit the Chans, it was because she was not allowed to. She had been emancipated from one family into the slavery of another. She mentioned what a good family she had grown up in, and she would rather be old and single and be a mui zai in the Chan's home. She loved Father very much.
I wrote about my grandma's Mui Zai in my book. I also remembered my mum almost got a Mui Zai too. It was after the World War Two. My great Grand Mother didn't want my mother to work too hard. So she bought a girl slightly older than my oldest sister. My father declined and packed the girl away. My Father's rationale was at this day and age, him being a Christian should not have a Mui Zai aka slave. How could he have the conscience of having a Mui Zai who slaves away while his own daughters went to school. Ah Tai aka Great Grand Mother argued we we just pay for her in the beginning and don't have to pay her anymore. Mother said we were feeding her. We knew about this returned Mui Zai when we had to do house work. We complained and wish we still had the Mui Zai.
The prounciation of my Quang Ning dialect MUI Zai, for a slave sounds like the sour plum, and MUI ZAI as a girl is different.
My parents had 6 girls, MOI ZAI SEE (bloody useless girls) as my Bodai (maternal grandma) would call us. She said, if we were in China, I would be sold off as a slave. I was the third girl. So would all subsequent girls. She also said my Dad had a Father-in-law look. When Father paid for me to study in Canada, Bodai said my fate was very good. I could have been a slave, instead I went to university.


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MCC steht für:
 China Metallurgical Group, chinesisches Bergbauunternehmen
 Mainzer Carneval Club, Mainzer Karnevalsverein
 Malabar Christian College, Hochschule in Calicut, Indien
 Marylebone Cricket Club, englischer Cricketclub
 Mathias Corvinus Collegium
 Mennonite Central Committee, internationales Hilfswerk der Mennoniten
 Mensurencorpsbuschenconvent, siehe Mensurconvent
 Mercator Research Institute on Global Commons and Climate Change (MCC) gGmbH ist ein wissenschaftlicher Thinktank, der mit sich den Herausforderungen des Klimawandels und der Nutzung globaler Gemeinschaftsgüter befasst
 Mesoscale Convective Complex, großräumiges Gewittersystem
 Methylcrotonoyl-CoA-Carboxylase, Enzym zum Abbau der Aminosäure Leucin
 Metropolitan Community Church, Dachorganisation von protestantischen Freikirchen
 Metropolitan Correctional Center, US-amerikanische Bundesgefängnisse
 Micro Compact Car, ehemaliger Automobilhersteller, siehe Smart (Automarke) #Gründung des Unternehmens
 Microcrystalline Cellulose, Hilfsstoff in der Pharmazeutischen Technologie, siehe Mikrokristalline Cellulose
 Millennium Challenge Corporation, Unternehmen zur Verwaltung des US-Entwicklungshilfefonds Millennium Challenge Account
 Mission Control Center, Flugüberwachungszentralen der Weltraumfahrt
 Mitsubishi Chemical Corporation, japanisches Chemieunternehmen
 Mobile Country Code, Länderkennung für die Adressierung von Mobilfunkstationen
 Mondragón Corporación Cooperativa, internationale Genossenschaft
 Motor Control Center, in der Regel ein normaler Leistungsverteiler (Motorabgänge, Umrichter etc.) – oft wird damit aber die Fertigung in Einschub- oder Stecktechnik gemeint.
 Mouvement des Citoyens pour le Changement (Bürgerbewegung für den Wandel), eine ehemalige belgische Partei, heute Mouvement Réformateur
 Mucociliar Clearance, Selbstreinigungsmechanismus der Bronchien, siehe Mukoziliäre Clearance
 Multiversion Concurrency Control, Konzept zum Ermöglichen konkurrierender Zugriffe auf dieselbe Datenbank
 Merchant category code, wird verwendet, um Unternehmen welche Kreditkarten akzeptieren nach der Art der von ihnen angebotenen Waren oder Dienstleistungen zu kategorisieren
 MCC Aviation (ICAO-Code), südafrikanische Fluggesellschaft
 römische Zahlzeichenfolge für 1200
  Madras Christian College, Kunst- und Wissenschaftskolleg mit Sitz in Madras, Tamil Nadu, Indien.
mcc steht für:
 Bitur (ISO-639-3-Code), Papuasprache auf Papua-Neuguinea

Abkürzung

redpajama_set_name: RedPajamaWikipedia


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    <title>What is the top differentiating attribute for squamous-like bladder cancer? — TumorMap Help</title>
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TumorMap Help

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What is the top differentiating attribute for squamous-like bladder cancer?¶

Objective¶

Identify squamous-like bladder cancer samples from the TCGA PanCan-12 mRNA dataset.

Skills Covered¶

Filtering the samples in the map using a short list selection

Creating a group using polygon selection

Sorting sample attributes based on their association with a custom group

Steps¶

With the map, Pancan12/SampleMap, make sure the mRNA layout is selected and only Tissue is selected in the Short List.

In the Short List Tissue entry click on the filter button  and be sure BLCA is selected in the drop down menu.

Observe that only BLCA samples are colored in the map.

Locate the zoom in/out control in the bottom right of the map and click on the + once or twice to zoom in to see all of the BLCA tumors as below.

Click on the Select main menu then click on by Polygon.

Select the region containing the squamous BLCA samples (those in the LUSC and HNSC region) by clicking to define each vertex of the polygon. Double-click to complete the polygon. Name this group squamous.

The above actually selects all of the samples within the selection region and not just BLCA. That's OK because we are most interested in the region.

Click on the Sort attributes button on the header as shown below.

In the window, Sort Attributes by Associative Statistic, be sure Focus attribute and Layout independent are selected as shown below. For Attribute A select squamous. Click the Sort button.

The message to the right of the Sort button will appear as below.

After a few seconds to a few minutes when the computations complete, the message will change to the below. Observe that one of the top differentiating attributes in the Select Attribute list is GP6_Squamous differentiation/development program. Click on this entry to display it and see its higher values in the area selected.

Table Of Contents

Overview

Get Started

Video Introduction

How do I view the Pancan12 TumorMap?

How do I create a map from my data?

How do I save and share my work?

How are colors customized and an image downloaded?

Which samples share a particular genomic alteration?

How are custom annotations created?

What is the top differentiating attribute for squamous-like bladder cancer?

Which genomic events distinguish one subtype of cancer from another?

Which genes and pathways drive clustering of basal breast tumors?

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redpajama_set_name: RedPajamaGithub


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Color Me Punk: Rubella Ballet Plays First L.A. Show Since 1985 at Echoplex
Rubella Ballet comes from a time when there weren't as many boxes for you to cram bands into. There was punk, metal, and a few subgenres in between. They were associated with the anarcho movement, namely Crass, in England in the late 70's and 80's but musically, their albums show more diversity than any label could commit them to. Their songs and look represented the essence of anarchy. Rebellion, even against itself. When everyone wore black, they wore Day-Glo. When everyone played fast, short songs, they experimented with slower rhythms and more nuanced arrangements. Singer Zilla Minx and guitarist Sid Ation still know how to get weird. For all these young kids born after Rubella Ballet's heyday, this was a show unlike anything they had ever seen.
Rubella Ballet's latest album, Danger of Death, brought them back to Los Angeles to play Part Time Punks at the Echoplex for the first time since 1985. Along with a few death rock, anarcho, and post punk bands, their combined effort made for a very special night of music.
related content: Gene Loves Jezebel Play Immigrant In Its Entirety At Part Time Punks Gig
Rubella Ballet
Spain's Fatamorgana was the first to take the stage. Made up of members of death rock band Belgrado, I had been waiting to see these artists perform for what seemed like forever. Unlike their death rock incarnation, Fatamorgana plays spellbinding electronic post punk that is light and made for dancing. Singer Patrycja Proniewska is not just a great singer but a young master of capturing various mythology within performance. You see her capture the movements and gaze of a robot at one moment then of a mime the next. Her voice is sweeping and sweet and she dances on light feet. With her silver shirt the performance took a futurist vibe that contrasted the low key performance with a high concept sheen. She's the musical Andy Warhol of contemporary death rock.
related content: Berserktown 3: The Craziest Festival On Earth
Lux, another band from Spain was, provided the most punk death rock music of the night. They played a good set with plenty of energy and punch. The goth tone in Kiara's bass was especially thrilling and Nina really killed it on vocals over the band's death rock blitz. You could sense the synergy between them. My only criticism of this band is that they sound a lot like so many other bands I've heard in LA. It's almost a standard sound for that scene, apparently in Spain too, oddly enough. As good as their music is, they still need to discover their originality.
related content: Los Crudos Play The Echoplex Right When L.A. Needed It Most
Readership Hostile is a young post punk/death rock band with veteran musicians and talent. The first thing you notice is Adrienne Pearson's fierce and visceral performing style that balances being savage as a beast and elegant, graceful and sexy as a woman. She brings out the true power under the death rock sound with an almost 80's pop swing. You see this band and assume you're watching legends but they only released their first EP in 2014. With a new single "Who Buried God?" in 2018, there seems to be little in the way of Readership Hostile taking over Los Angeles' underground goth scene.
related content: The Sound of Sex: Boy Harsher Seduces The Echoplex
Readership Hostile
Rubella Ballet brought all the true punks out of the woodwork. Musicians, street urchins, and vinyl collectors alike were in attendance to see this historic set. As soon as Sid Ation walked on stage covered in Day-Glo paint from head to toe. Neon streaks everywhere form his shoelaces to his guitar strings, I knew this was going to be a strange sight to behold. This was Liquid Sky turned music, Liquid Sky on Ice! Black clothes are punk but being dressing like an absolute weirdo is what this scene was made for.
As soon as the lights dimmed and darkness befell the stage to let that colors glow, making the goths subconscious their cum stains would appear on their clothes, Zilla took the stage and the debauchery began. Their age didn't slow their performance down one bit, it was intense and ripping from start to finish. This was some of the most youthful live music I've heard all year. There was something so cutting edge about it all.
The way John Waters says being gay is not enough anymore to be weird, well, this was enough. This was so inherently strange and rebellious it was inspiring to watch. Rubella Ballet truly doesn't care.
Related Items:anarcho punk, death rock, echoplex, fatamorgana, featured, goth, lux, part time punks, post punk, readership hostile, rubella ballet, the echoplex
A Cathedral of Hardcore: Ceremony at Echoplex
Cyber-Core: Vein. FM at the Echoplex
Photo Recap: Pink Turns Blue at Echoplex
Take This: Win 2 Tickets To Hercules and Love Affair at El Rey
Rafael Reyes of Prayers Interview: On Fatherhood


On Tuesday 26th March, happy members of the Metropolitan Mess gathered in the plush surrounds of the Grange City Hotel, to be greeted by their President, R.W. Bro. Sir Michael Snyder, the Metropolitan Grand Master.
After the delight of a very enjoyable champagne reception, where Met Mess members mingled with DepMetGMs, AMetGMs, MetGInsps, SVO's, VO's and friends, they sat down to an excellent 3-course lunch and wine and were then splendidly entertained by Alfie Moore.
Alfie, a recently retired police officer, had the audience howling with laughter as he regaled them with hilarious anecdotes, drawn from his interesting career on Humberside. Having spent 18 years in the Force, Alfie traded his truncheon for a microphone and sprang sideways into stand-up comedy, appearing on numerous TV and Radio shows as well as at the Edinburgh Festival.
The Metropolitan Officers Mess convenes just twice a year, once for a spring-time luncheon and then a dinner in the autumn.
£10 Membership is open to all Metropolitan Officers, present and past, as well as Senior Visiting and Visiting Officers.
The amazing list of guest speakers has ranged from Lieutenant-General Sir Michael Rose – UNPROFOR, Sir Christopher Meyer – UK Ambassador to the USA, Michael Portillo – former MP, Cabinet Minister and broadcaster, to Jimmy Greeves, former England International footballer.
The next Met Mess is a dinner on Thursday 26th September at the Grange City Hotel.


text:
← Franchise Weekend – Nymphomaniac Vol 2 (2013)
REVENGE is released on Digital HD on 7th September →
ABC Film Challenge – Sci-Fi – F – Fire in the Sky (1993)
Director: Robert Lieberman
Writer: Tracy Torme (Screenplay) Travis Walton (Book)
Starring: D.B. Sweeney, Robert Patrick, Craig Sheffer, Peter Berg, Henry Thomas, Bradley Gregg, James Garner
Plot: An Arizona logger mysteriously disappears for five days in an alleged encounter with a flying saucer in 1975.
Tagline – For five days, a man was borrowed. The story that Travis Walton and five other witnesses told is so unbelievable, so unimaginable, that it has become the most famous case of UFO abduction ever recorded.
Runtime: 1 Hour 49 Minutes
Verdict: That One Scene Shines
Story: Fire in the Sky starts as five loggers Mike (Patrick), Allan (Sheffer), David (Berg), Greg (Thomas) and Bobby (Gregg) return to town in a panic with a story to tell the authorities, which brings in Sheriff Frank Watters (Garner). The story the guys tell is the they investigated a mysterious light in the woods which took Travis (Sweeney).
With the search going on for Travis, Mike and the crew find themselves prime suspects in a cover up, until Travis returns with his own account of the story.
Thoughts on Fire in the Sky
Characters – Travis Walton was a logger that went missing for five days, he doesn't remember at first what happened, but the truth ill haunt him for the rest of his life as he remembers being taken by aliens. Mike Rogers is the best friend of Travis' that doesn't know how to handle his disappearance and his reappearance, but knows that nothing was done by the men in his crew. Frank Watters is the sheriff trying to prove this is a hoax or a cover up depending which side of the events are. The characters are based on the real people which does help make this feel like a good real story to follow.
Performances – The performances across the board are good, with D.B. Sweeney showing the pure terror needed during the abduction scene. Robert Patrick works well in his role where he could easily be the lead of the film. James Garner brings an extra bit of class to the performances too.
Story – The story follows one of the most famous and detailed encounters from alien abduction in history. The story first treats the events like a murder cover up which is wonderful to watch on the screen because we don't need to watch a full abduction routine which we do see later in the film. the story balances the ideas of what could be truth and what people would want the truth to be too.
Fantasy/Mystery/Sci-Fi – The fantasy part of this film comes from what you want to believe about the events, because there is no proof it is real, this adds the mystery too which does also circle around the truth too. The sci-fi side of the film comes from the moment in space which shows what the aliens did to Travis.
Settings – The film keeps us in the small town location where the events happened which does keep us understand how the town would react to the situation.
Special Effects – The effects are spared for the final moments of the film where we see what happened to Travis, they all look great too.
Scene of the Movie – Memories nightmare
That Moment That Annoyed Me – I was expecting more from the alien abduction side of things.
Final Thoughts – This is a good sci-fi film, it does balance the event between what could have happened, what is believed to have happened and leaves you to make up your own mind, with a sequence in the ship which will be haunting.
Overall: A sci-fi film that hasn't dated like others
By Darren Lucas • Posted in 1993, F, Fantasy, Mystery, Sci-Fi	• Tagged Bradley Gregg, Craig Sheffer, D.B. Sweeney, Henry Thomas, James Garner, Peter Berg, Robert Lieberman, Robert Patrick, Tracy Torme, Travis Walton


For people who once in a while like to play games on their smartphone it perhaps is one of the important smartphone related questions: what are the top mobile games for my smartphone? This list will get you up to date with the best mobile games of this moment!
This is a game that is brilliant in its simplicity. Your goal is to cross the road, which doesn't end by the way. The more you advance, the more difficult Crossy Road gets, which keeps the game a challenging one. Crossy Road is regarded as one of the best mobile games of the moment. The game is developed by indie developer Hipster Whale and published by Beijing based Yodo1 Games. On Facebook Crossy Road has almost 15 thousand likes. Yodo1 Games has over 700 likes and the company is quite regular with their status updates.
This is a first person shooter, published and developed by the well-known developer Gameloft. Nova 3 is available for free for iOS and Android. Nova 3 is one of the best mobile games in terms of first person shooting. Fight your way through various beautifully designed levels with futuristic weapons. Nova 3's Facebook page seduces you to download the game. On its website, Gameloft gives you even more impressions about Nova 3's gameplay, notably with video's.
Developed by EA Sports and easy to find in the Apple App Store and Google Play Store. This is a game where you can race on real circuits with real existing cars. Always wanted to try the Bugatti Veyron but lack the funds to do it? Try Real Racing 3! The game can easily be regarded as one of the best mobile games available. Over a million other players are playing this game on a monthly base. You can earn money by winning races, but it is also possible to buy money or coins to upgrade your cars quicker. You should know, however, that the game is easy playable without paying.
You prefer to lean back and play an easy game? Try Skyward, a top mobile game. Pure and simple! The game is developed by Ketchapp and is available for both Android and iOS. On its Facebook page, Ketchapp keeps you posted about upcoming updates for Skyward. Ketchapps Twitter page is more ore less a retweet channel for fans opinions about Ketchapps games.
This is a game that is perfect for gamers who like to lean back and don't want to concentrate so much. Thus, Subway Surfers is very easy to play. You play a young graffiti artist who is being followed by a police officer who wants to catch him. To escape, you must find a way through the railway system. Be careful! Otherwise a train hits you. Along the way you can pick-up coins and objects that helps you get away. Subway Surfers is available for Windows Phone, Android and iOS. Subway Surfers is developed by Kiloo Games and has over 10 million monthly players. Kiloo's website is very nicely designed and it gives you a good impression about Subway Surfers' gameplay. It is easily to say that is one of the best mobile games around in terms of popularity.
This game will probably remind you of Super Mario. It is one of the top mobile games to frustrate you. You have to make your way through various levels by jumping over objects. It is one of the most popular games at this moment for iOS. Developer 1button has a simple, clear website with information about its games. While 1button and Mr Jump don't have very big Facebook pages, the game was promoted by Apple's App Store Facebook page.
This is one of the best mobile games for girls. You can play this game both on your Android, Windows Phone or iOS smartphone or tablet. King.com Limited developed this game. There are various editions of Candy Crush, which keeps it entertaining and challenging. Candy Crush is on the brink of 75 million likes on Facebook and it truly is one of the top mobile games for girls.
This top mobile game reminds you of Tetris! It gained popularity in a very short time, maybe because of its nice Facebook page. Gram Games developed this game for girls and boys. This company approached its marketing very clever. The website is clear and you can view 1010!'s gameplay trailer immediately. 1010! can be one of the best mobile games in terms of popularity and simplicity this year.
Miss those times where you grabbed a beer with your friends and played pool at the local bar? Try 8 Ball Pool. It reminds you of the good old times. 8 Ball Pool is one of the best mobile games in terms of popularity. Also, the game is beautifully designed. You play against people from all over the world. This is a game for both girls and boys from all ages. The well-known company Miniclip developed this game. You can play 8 Ball Pool on Android, iOS and your computer. 8 Ball Pool's Facebook page has over 13 million likes and gives you regular updates about people playing the game.
These are the top mobile games that you need to have played before you die! Try them out and let us know your opinion! Our favorite is defenitely Subway Surfers! Which one is yours?
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Loughborough Sport's Murphy joins England Netball's coaching team
Image provided by Ben Lumley photography.
Loughborough Sport's Murphy joins England Netball's coaching team 7 May 2021
England Netball has today welcomed Loughborough Sport's Olivia Murphy to its Vitality Roses backroom team as its new Technical Coach.
Whilst remaining in her current role as Head of Performance Support at Loughborough, Murphy's new part-time dual role will see her play a pivotal role in developing and preparing England players through expert coaching and support on training camps and in competitive series.
Murphy – who is also a Loughborough alumna and a member of the University's Hall of Fame – joins the England coaching team having amassed 95 caps across 15 years playing internationally for England, with six years spent as senior captain.
With over 20 years of coaching experience, Murphy has helped ready players on the developmental pathway to their international careers as England Netball Technical Coach for the U17 age group since 2014 and at many a club setting including Loughborough Lightning where she is currently the Vitality Netball Superleague side's Assistant Coach.
"It's a privilege and an honour to be involved with the Roses in this role. With the netball season firmly underway it has been a great showcase for the athletes but also for me to start to look at ways to help them develop," commented Murphy.
"Ultimately, I'm so excited to work with the talented staff and players and cannot wait to get started."
Murphy playing for Loughborough Lightning in the 2007/2008 season. Image: Nigel Farrow.
Murphy will join Sonia Mkoloma (Assistant Coach) and Liana Leota (Technical Coach) in Head Coach's Jess Thirlby's new-look backroom team.
All three coaching additions are experienced medal-winners at multiple Commonwealth Games and Netball World Cups and will work with the Vitality Roses until post the 2023 Netball World Cup.
As the countdown to the 2022 Commonwealth Games in Birmingham next summer begins, the appointments bring huge experience and netball DNA to the heart of the off-court team supporting the Vitality Roses as they look to repeat history on home soil.
Jess Thirlby, Vitality Roses Head Coach, said:
"If you know netball, you know Liv, Sonia and Liana. Their passion and collective experience from playing and coaching at the highest level over many years in multiple high-performance environments across the world will be a huge asset for the Roses as we prepare for Birmingham 2022.
"I'm so excited about what they will bring to our team. Liv was my captain when I first got into the England team, we played at the Manchester Commonwealth Games together in 2002, and we also worked together with the England A and England U21 teams in 2013, as well as at Team Bath for a season and most recently with the Roses."
Thirlby added:
"We're very much looking forward to having them join us in our first England camp this summer. They are world-class in their own right and the dynamic they will add to our environment, to me and the team will be invaluable.
"I also want to acknowledge and thank England Netball for their continued support and investing in this pioneering Roses coaching team, demonstrating the importance and impact that great coaching can have on a team with the ambition that we do as Roses."
Press release reference number: 21/74
Loughborough is one of the country's leading universities, with an international reputation for research that matters, excellence in teaching, strong links with industry, and unrivalled achievement in sport and its underpinning academic disciplines.
It has been awarded five stars in the independent QS Stars university rating scheme, named the best university in the world for sports-related subjects in the 2020 QS World University Rankings and University of the Year by The Times and Sunday Times University Guide 2019.
Loughborough is in the top 10 of every national league table, being ranked 7th in the Guardian University League Table 2021, 5th in the Times and Sunday Times Good University Guide 2020 and 6th in The UK Complete University Guide 2021.
Loughborough is consistently ranked in the top twenty of UK universities in the Times Higher Education's 'table of tables' and is in the top 10 in England for research intensity. In recognition of its contribution to the sector, Loughborough has been awarded seven Queen's Anniversary Prizes.
The Loughborough University London campus is based on the Queen Elizabeth Olympic Park and offers postgraduate and executive-level education, as well as research and enterprise opportunities. It is home to influential thought leaders, pioneering researchers and creative innovators who provide students with the highest quality of teaching and the very latest in modern thinking.
UniversitySport
Dan Trussell
Sport PR Officer


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We install Cat5e and Cat6 cable, as well as coaxial cable. These cables will satisfy the needs for internet, phone, and TV solutions. Every install will come complete with a rack (recommended 2-post or wall mount) and cables being punched into a patch panel.
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text:
Michiko Gupta
Senior Digital Strategist
Instagram has been experimenting with hiding "likes" on its platform in recent months, and it has gradually started to roll out this feature in the United States. Removing something that has been so critical to Instagram's success is a BIG deal — and there has been a lot of conversation buzzing about it since the announcement.
" Anything that tech companies can do to ramp up the good and tamp down the bad is bold and positive."
Tweet this Share this on Facebook
Why is Instagram doing this?
It's trying to become the safest social media space on the internet— especially for teens and members of Generation Z—says Instagram CEO Adam Mosseri. By dropping the total number of "likes," Mosseri hopes to "depressurize" the platform to make it less competitive and more welcoming.
Watch Mosseri discuss the new policy at a November tech forum.
A BOLD MOVE
As a digital strategist, I've seen the good that social media can do. I've also seen the bad. So anything that tech companies can do to ramp up the good and tamp down the bad is a bold and positive move.
To be clear, you'll still be able to see "likes" on the back-end, but they won't be visible to everyone. Therefore, it should remain an important part of the engagement metrics such as comments and video views to better understand what content resonates best with your audience.
HIGHER ED IMPLICATIONS
As higher ed communicators with a primary target audience comprised of teens and Gen-Zs, what does it mean for them?
I spoke with two higher ed communicators—Lawerence Synett, director of social media at Carnegie Mellon University; and Priscilla Morales, a digital communications specialist at the University of Pittsburgh—to hear what they think about the new policy.
Synett: Research has shown that teens evaluate their self-worth through vanity metrics such as "likes," as well as comments (or lack-there-of) from followers. Now, is this particular move going to completely solve the problem of social gratification through posts on social media? No, but it is a step in the right direction.
Morales: Instagram is just one of the many tools we use to help move students through the admissions funnel, and we know that "likes" alone aren't enough. We're focused on more meaningful interactions. That said, I think the new policy is a good thing for the younger generations who constantly focus on social comparison.
Both communicators said connecting and actually engaging with followers is much more important than collecting "likes."
Synett: Our Carnegie Mellon audience is unique—it's a mix of arts and science, and everything in between—which is a source of pride for our community. It's our responsibility to move beyond "likes" and engage with our audience, to always provide opportunities for them to interact across all our digital channels. That's the reason social media exists.
Morales: People come to Instagram or our other platforms to see what it's like to be a student at Pitt, not to count how many "likes" we have. We're different than a fast food chain or retail store, where "likes" can be a helpful metric because the commitment level is much lower. A prospective student needs to make a much deeper connection with a college or university brand.
What are your thoughts? Has your organization been impacted by the change? Let me know at mgupta@lipmanhearne.com.
Categories: Education, Membership Organizations, News


We believe our clients are the most important component of our business.
It is therefore our priority to continually strive to improve and deliver services in a way that our clients want and appreciate. Our Client Steering Committee helps guide us to deliver this.
Our Client Steering Committee is at the heart of our business.
Part of our ongoing service includes a feedback questionnaire – so each and every client has an opportunity to have their thoughts and suggestions heard. We endeavour to implement your suggestions to deliver services our clients want.


You may not think of Sundance as being a bastion of films that skew scientific, but there are a surprisingly large number of them each year. This did not go unnoticed by U human genetics professor Gabrielle Kardon, who immediately saw an opportunity to blend two of her passions. She and an editor with Science magazine have partnered to create Science@Sundance to review science-themed films, enlisting the help of U biology professor Michael Shapiro and a small cadre of colleagues from other institutions.
Gabrielle Kardon, professor of human genetics (right), partnered with an editor from Science magazine to create Science@Sundance to review science-themed films. Michael Shapiro, professor of biology (left), and other scientists will also become movie critics during the festival.
Their mission is to highlight movies that excel at using art and storytelling to engage their audiences in important science-related issues. The ultimate goal? To stir excitement and awareness, and encourage scientists to think about pursuing the arts as a way to get the word out about subjects that matter to them.
Look for their reviews in Science magazine in February. In the meantime, you can experience the Sundance Film Festival through scientists' eyes on Twitter @ScienceSundance.
If you're lucky enough to score Sundance tickets, look for these films below. Whether you're a nerd at heart or simply sci-curious, there is something for everyone.
The inspiring story of 13-year-old William Kamkwamba, who developed his own windmill to bring electricity to his rural community in Malawi. Based on Kamkwamba's autobiography and directed by Chiwetel Ejiofor. 2019 Alfred P. Sloan Foundation Feature Film Prize winner.
Featuring never-before-seen NASA audio and film footage, this documentary brings to life the Apollo 11 mission, which landed the first two people on the moon. Directed by Todd Douglas Miller.
Elizabeth Holmes arrived in Silicon Valley promising a medical revolution, but instead delivered an empty fraud. The spectacular rise and fall of Theranos is documented by director Alex Gibney.
Vaquitas are the world's smallest whale, but their home in the Sea of Cortez is a battleground that has brought them to the brink of extinction. The vaquitas are the collateral damage in the illegal harvest of totoaba fish, "the cocaine of the sea," a multimillion-dollar business. This film uncovers the harrowing battle between the Mexican cartels, Chinese mafia, environmental activists, Mexican navy and undercover agents. Directed by Richard Ladkani.
Tigers have been decimated by poachers, but in Russia Pavel Fomenko and in India Kailash Sankhala lead heroic efforts to save these venerable animals. Directed by Ross Kauffman.
"Take half, but leave half to the bees." What happens to the natural balance when nomadic beekeepers break this cardinal rule? The last female bee-hunter in Europe is left to save the bees. Directors Ljubomir and Tamara Kotevska.
**Banner image: Buzz Aldrin, part of the Apollo 11 team in 1969. Courtesy of NEON CNN Films.


which I tried but doesn't work.
The requested availability zone is not available (HTTP 400).
Availability Zone is set in Horizon), it will create VM in random AZ.
nova services. I think this should work.
where there is available capacity.
I need a help with AZ in Nova. I think that this case wasn't discussed before, I tried hard to google it and I find only one solution (below) which I tried but doesn't work.
I want Nova to behave like that when Availability Zone is not specified (Any Availability Zone is set in Horizon), it will create VM in random AZ.
In nova.conf I have set default_schedule_zone = None and restarted all nova services. I think this should work.
However, most operators leave this at its default setting (None), because it allows users who don't care about availability zones to omit it from their API call, and the workload will be scheduled to any availability zone where there is available capacity.
I am using stable Pike release installed via Openstack-Ansible on Ubuntu 16.04.


text:
Q: Multiplicity constraint violated Entity framework 6 I have something which i don't understand. This is a similar case like mine.
I have BaseEntity, Product, Supplier, Contract and a ProductSupplierForContract Entities, all inherit from BaseEntity.
Base Entity:
public class BaseEntity
{
    public int ID { get; set; }
    // other properties that are not entities by themself
}

Product Entity:
[Required]
public ICollection<Supplier> Suppliers { get; set; }

[Required]
public ICollection<ProductSupplierForContract> ProductSupplierForContracts  { get; set; }

public  ICollection<Contract> Contracts { get; set; }

Supplier Entity:
public ICollection<ProductSupplierForContract> ProductSupplierForContracts  { get; set; }
public ICollection<Product> Products { get; set; }

ProductSupplierForContract Entity:
public string ProductnumberValue { get; set; }

public Supplier Supplier { get; set; }
public int Supplier_Id { get; set; }

public Product Product { get; set; }
public int Product_Id { get; set; }

public Contract Contract { get; set; }
public int? Contract_Id { get; set; }

Contract Entity:
[Required]
public ICollection<Product> Products { get; set; }
public ICollection<Supplier> Suppliers { get; set; }
public ICollection<ProductSupplierForContract> ProductSupplierForContracts { get; set; }

Following scenario must be possible and allowed:
1 Contract can hold multiple instances of 1 product BUT from a different supplier.
Therefore i created the ProductSupplierForContract (PSFC) Entity which would hold this relation of 1product-1supplier-1productnumber (value) -1contract .
When i edit 1 existing product that already has 1 PSFC instance, and add another instance of PSFC holding another PK, ProductId & SupplierId, i get this error:
{"Multiplicity constraint violated. The role 'ProductSupplierForContract_Product_Target' of the relationship 'ContractCare.Models.ProductSupplierForContract_Product' has multiplicity 1 or 0..1."}

I don't get why because isn't it so that i can have:
PSFC 1
PK 1
ProductId 1
SupplierId 1

PSFC 2
PK 2
ProductId 1
SupplierId 2

Why should i (like described in the linked post above) have a many to many relation  PSFC on Products?
Thank you for any feedback!
Kind regards.

A: Following link might help you more in this problem!

*

*Multiplicity constraint violated. The role '...' of the relationship '...' has multiplicity 1 or 0..1

A: Extending on the link that Aparna Gadgil provided, one of the comments posted by Arturo Hernandez resolved the error that i had so i am going to mark this solved since my original issue was the error.
Quote:

The problem is that detachedChild.parent should be assigned
  attachedParent.

foreach(var detachedEntity in detachedEntities)
{
 attachedEntity.ChildEntities.Add(detachedEntity);
 detachedEntity.ParentEntity = attachedEntity;
}

In my case, i had to attach the parent entity (product we are updating) to the ProductSupplierForContract.Product. Creating the new entity like suggested in the answer was already the case for me, and other things like attaching it and so on didn't fixed it for me.
Since i didn't post any code on how i was performing this action this wasn't very helpful to be able to help me and i will surely try to be more specific in the future :).
Kind regards and thank you!

redpajama_set_name: RedPajamaStackExchange


text:
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Immunization (Generation of antibodies)
An antigen can be almost any molecular substructure (i.e. part of a molecule). Antibodies are proteins, which specifically bind to an antigen and are produced by specialized white blood cells (B-cells) of the immune system upon contact with foreign (i.e. not self) antigens.
This defense mechanism against pathogens can be exploited to produce antibodies against any molecule of interest. Repetitive immunization with an antigen will generate an immune response and lead to the production of many B-cells that produce vast amount of antibodies directed against the antigen. These antibodies can be used in the laboratory to detect specific antigens (e.g. pregnancy test).
The serum (i.e. antiserum) of the blood of the immunized animal contains the antibodies, which can be used for many different techniques. Antisera are often called polyclonal antibodies.
It is also possible to isolate the antibody-producing B-cells from the animals to fuse them with a cancer cell line. The cell hybrids are called are called hybridoma. The technique also allows selection of successfully fused B-cells, which produce the antibody of interest, and indefinite expansion in cell culture. As the hybridoma secretes its antibody into the extracellular environment, the cell culture supernatant contains the antibody of interest. Because a hybridoma secrets one type of antibody originating from one B-cell, these antibody preparations are called monoclonal antibodies.
Immunofluorescence – IF
Immunofluorescence is a method to visualize antigens of cells by using antibodies.
Antibodies can be used to probe cells to test whether the antigen is present. The cells can be freshly isolated primary cells or cells in cell culture. Specific antibodies that detect the antigen of interest can be generated by immunization. To visualize the antibodies bound to the antigen, the antibody can be labeled in various ways. Most common are fluorophores or enzymes as labels. Usually a so-called secondary antibody is labeled, which detects the primary antibodies bound to the antigen of interest. This results in a sandwich composed of the antigen that is bound by the primary antibody, which is in turn bound by the secondary antibody that carries the label for detection. Fluorescent labels can be detected using fluorescence microscopes and enzymes as label can be detected by their enzymatic activity, which is a color reaction.
Next Generation Sequencing – NGS
NGS is a method that allows the sequencing of millions of DNA templates in parallel in a cost-efficient and fast manner.
Because many different NGS protocols from different companies are around and due to the complexity of the sample preparation and sequencing process, only one widely used NGS system (Illumina®) is explained here.
The DNA of interest is first chopped in to smaller pieces of a relatively uniformed size and are ligated with adaptors to which the primers will bind. These fragments (the library) are attached to a solid carrier for example a glass slide. The billons of different DNA fragments are then clonally amplified on the carrier. Sequencing is performed in real-time by adding a DNA polymerase and reversibly fluorescently labeled nucleotides. For each base of the millions of different templates that is to be sequenced, the polymerase will add the respective nucleotide. Then the whole glass slide is excited by laser light, which will lead to a fluorescent signal for each newly added base of each fragment (as for Sanger sequencing, the four bases again have four different colors) and a picture is taken. Then the label is removed and the process is repeated many times. The resulting reads are between 50 and 250 base pairs. The total amount of sequence is around some billions of base pairs, which are highly redundant. The reads are assembled usually using a reference sequence to which they are mapped.
RNA interference (RNAi)
RNAi is a method to specifically inhibit the expression of genes at the mRNA level by short doublestranded RNA molecules complementary to the gene of interest, which govern the degradation of the respective mRNA.
RNA interference was discovered by Andrew Fire and Craig Mellow in 1998, which received the Nobel Prize Medicine for this groundbreaking work. They discovered that introduction of doublestranded RNA (dsRNA) led to specific degradation of mRNA that match the sequence of the dsRNA in the roundworm C. elegans. This pathway is conserved in many eukaryotes and represent an additional level of gene expression regulation.
The cell itself expresses so called micro-RNAs (miRNAs) that target and silence the expression of groups of genes. Exogenous dsRNAs are usually introduce experimentally and are designed to knock down specifically the gene of interest. Note that miRNA transcripts do not encode for proteins.
Both types of RNAs are processed by an enzyme called Dicer, a ribonuclease, which cuts the RNAs into short fragments of 20 to 25 nucleotides in length, which are called short interfering RNAs (siRNAs). The strands of the siRNAs are separated and are integrated in an enzymatic complex named RNA-induced-silencing-complex (RISC). The RISC complex scans mRNAs for complementarity to the siRNA strand and if complementarity is detected, endonucleases of the Argonaut protein family degrade the mRNA preventing thereby expression of the protein.
qPCR – quantitative reverse-transcription polymerase-chain-reaction
The method is based on three more simple techniques, which are polymerase chain reaction (see PCR), reverse transcription and detection of DNA by fluorescent signals.
Reverse transcription is the process that converts RNA into single stranded DNA, a so called copy DNA (cDNA). A reverse transcriptase is an enzyme with a RNA-dependent DNA polymerase activity and is widely used to convert mRNAs (messenger RNA, protein coding) into cDNA. Because mRNAs have a long stretch of adenines at their 3'-end (poly-A), they are often reverse transcribed using a primer composed of a long stretch of thymindines (poly-T). Thus a cDNA-preparation often contains the totality of all protein-coding mRNAs from the tissue or cell type from which the RNA was isolated.
There is also a large number of non-coding transcripts, which are as important for a cell as the protein-coding mRNAs. The most prominent examples are ribosomal and transfer RNAs that are crucial for protein synthesis. Another prominent member of these transcripts are micro RNAs, which are only known since 15 years, that mediate posttranslational control of gene expression.
Quantitative reverse transcription PCR is a highly sensitive, quantitative method that allows the quantification of expression levels of RNAs (i.e. how much is there in a cell). qPCR simply detects the amount of DNA generated in each PCR cycle by fluorescent signals. There are several technical variants to detect DNA. The most simple form is to add a fluorescent dye to the PCR reaction, which can only bind double stranded DNA but not to nucleotides or single stranded DNA (such as primers or cDNA). The fluorescence is directly measured in the reaction plate in the qPCR machine.
A microarray is a technique to detect changes in gene expression of thousands of genes in parallel.
Microarrays are usually used to analyze the transcriptome of a sample. The transcriptome of an organism is the entirety of all genes expressed during its life time including embryogenesis. A microarray relies on specific probes, which are short pieces of chemically synthetized DNA complementary to the gene of interest. For each gene present on the microarray several little spots with the probe are attached to a slide. That means if the transcriptome of an organism is about 25.000 mRNA, the array would have about 100.000 spots in total, if four spot are printed per gene.
To analyze the transcriptome of a sample, the RNA needs to be isolated, reverse transcribed into cDNA (see also qPCR) and labeled fluorescently. Because the probe can bind extremely specifically to its target mRNA, the fluorescent labeled cDNA can now be hybridized to the microarray. After binding, the microarray can be washed and imaged using an automated fluorescent microscope. Because each spot contains some billions of probe molecules, the fluorescent signal intensity does not only tell whether a gene is expressed but also how much. Because each potentially expressed gene is present as probe on the microarray, the activity of virtually all genes in the sample compared to the control can be accessed at once.
The signals are then normalized to controls such as house keeping genes, which allows quantification and comparison of different samples. Microarrays have been proven to be an extremely versatility and useful tool in molecular biology.
FISH is a method to detect expression of RNAs in cells of tissues with high spatial resolution.
FISH exploits the property of single-stranded RNA/DNA molecules to form double-strands with respective complementary RNA/DNA molecules. Often RNA probes are used to detect specific transcripts.
To investigate the expression of an gene of interest in a tissue of interest, the gene has to be cloned into a vector that contains a RNA polymerase promoter. Alternatively, the RNA-polymerase promoter can added to the gene directly by a PCR reaction.
The RNA-probe is generated by in vitro translation, which yields RNA molecules complementary to the RNA of interest. In addition, some of the nucleotides are labeled usually with digoxigenin. The RNA-probe can then be incubated with a section of the tissue or cells of interest and it will bind to the respective RNA. The label can then be detected using antibodies, which are in turn labeled fluorescenct dye. After washing away free-probe, the expression of the respective RNAs can be visualized by fluorescence microscopy.
Cell culture is a method to culture cell lines or primary cells in vitro. Cells in cell culture are accessible and easy to manipulate making experimental setups convenient.
The basic principles of cell culture were already established beginning of the 20th century, when developmental biologists started to culture organs and embryo explants in salt solutions supplemented with body fluids such as amniotic fluid, allantois extract or serum. In the 50ties of the previous century the widespread virology research spurred the development of robust cell culture protocols for mammalian cell lines, which were used to produce viral particles for research and vaccine development.
Cells freshly isolated from the body are called primary cells. The cells that can be isolated most easily from mammals are cells of the peripheral blood. The isolation of cells from tumors and subsequent long term culture or infection of primary cells with tumorigenic viruses lead to the generation of many cell lines, which are virtually immortal and can be cultured much easier than primary cells.
Establishing cell culture conditions was and is still trail and error by just testing out many different ingredients and conditions, and is usually very complex. For a long time the biological reasons for many ingredients that support the growth of a particular cell type were unclear or as for serum the active ingredients were just unknown. However, since the 1950ies we learned a lot about a cells biology and understand much better what a cell need to be happy. Yet, out of the approximate 200 different cell types of the human body only some can be cultured with ease. To reduce experimental variability, there are a lot of efforts to get rid of variable and undefined ingredients such as serum and instead to move towards chemically defined medias in which the concentration of all components, ranging from salts, organic chemicals, amino acids, peptides and proteins, are exactly known
But if you are a scientist nowadays working with mammalian cells, cell culture is in fact a routine job. Basically all media, supplements and dishes can be purchased commercially. For all cell lines and many primary cells robust standard culturing protocols are available.
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Guide your charachter using jet bag to collect golden coins. The more coins he will be able to get, the better equipment he can aquire to make it more fast and manageable. But there are amy danger in stock for him: meteors, and landing itself, because to stop on the earth surface he can stop only in one green uniform!


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Coronation Street's Sally Dynevor has a well-known daughter who stars in Netflix's Bridgerton
Acting obviously runs in the family!
Corrie legend Sally Dynevor is the mum of Bridgerton star Phoebe Dynevor (Image: ITV)
Since its release on Christmas Day last year, Bridgerton has become the centre of adoration, criticism and controversy.
The show follows the lives of the powerful Bridgerton family, whose eight young siblings are on a mission to find love.
Critics have praised creator Chris Van Dusen for Bridgerton's boldness, its alternative setting of a racially integrated Regency-era London and its depiction and exploration of female sexuality.
B&M release Bridgerton collection to get regency style at home this Valentine's Day
Historic London hotels that will make you feel like a character from Bridgerton
That said, some of the series' biggest moments have proven controversial - particularly a sex scene in which Daphne Bridgerton, one of the show's main characters, arguably violates the consent of her partner.
Daphne is played by Phoebe Dynevor, who began her career as a child actress in the BBC school drama Waterloo Road.
But what you may not know is that the 25-year-old comes from the stock of British soap superstardom.
Phoebe's mother is, in fact, Corrie legend Sally Dynevor, who plays pushy mum Sally Webster on the show.
Phoebe plays Daphne Bridgerton in the critically-acclaimed series (Image: Liam Daniel/Netflix)
Sally is reportedly very proud of her daughter's success in the Netflix series.
But Phoebe recently revealed that she had to skip her sex scenes while the two watched the show together.
She told the Daily Star: "I had to sit there and fast forward on high alert with the remote control. We managed it just about."
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Bridgerton has already been renewed for a second season, and will begin filming in the spring of 2021.
We can't wait!


"The Garden Gîte" is decorated to a high standard, sleeps 4-5 people, and has an open plan lounge, kitchen and dining area. There are 2 toilets, a bathroom, and 2 bedrooms, one with a double bed and built-in wardrobe, and one with 2 single beds, a private shower room, and a built-in wardrobe.
Dining area: Table and 6 chairs.
5. Visit our facilities & attractions page for more details on what's available on the property.


Sensitive Building's is about understanding as much as it is about creating. To that end, Rob Faludi, our professor and ITP Alumni, first assigned us to simply observe.
After chatting with Michelle Boisson, my classmate and partner on this project, we decided to try to head down Ground Zero on the anniversary of the 9/11 attacks to observe people as they experience the site. We first arrived at the Cortland Street Station but we meandered south from there to the edge of Zucotti Park, on the corner Trinity Place and Liberty Street where last year Occupy Wall Street had encamped.
We had originally planned on going straight to the memorial just a block away but it was closed to the public for family of the victims of the attacks. This turned out to be fortuitous since a second part of our observation exercise was to have another group from class go to the same location at a later date and observe what they could. Our counterparts, Michael Uzzi and Gavin Hackeling decided to attend on the following Sunday and Monday which happened to be the one year anniversary of the Occupy Wall Street movement.
Our observations can be seen in the presentation below.


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Critical Text Review: David Hackett Fischer, Albion's Seed: Four British Folkways in America
by Kevin Bair | Apr 6, 2021 | General | 0 comments
This is my Critical Text Review of David Hackett Fischer's 1989 book, Albion's Seed: Four British Folkways in America, New York Oxford: Oxford University Press. This is one of two reviews for the class HIUS_911 Comprehensive Examination and Readings in Early America.
David Hackett Fischer's book, Albion's Seed provides a unique look at the founding of America. He has a centralized theme of "British Folkways", where he evaluated four early British migration waves, where each group arrived from distinct British regions between 1629 and 1775. These diverse clans landed with distinctive and differing folkways, i.e., shared beliefs and practices, they spoke English, followed the Protestant religion, dwelled in similar manner, and embraced British liberties and laws.
These varied groups settled in four geographical areas. First, 1629-1640, Puritans from East of England to Massachusetts. Second,1642-1675, from the South of England, a few Royalist elite and many indentured servants settled in Virginia. Third, 1675-1725, immigrants moved to the Delaware Valley from Wales and North Midlands of England. Fourth, 1718 to 1775, found this group from the boundary of Northern Ireland and North Britain heading to live in the Appalachian backcountry. Each group shared the influences of their mother country and their varied regions on this nation (6).
Furthermore, Fischer indicated these external influences shaped this country, and claimed the South and its politic structure did not create slavery, nor did slavery create the South, but that the institution of slavery was brought to this country from Wessex, where it was practiced on a large scale and had existed since the Middle Ages (241). Moreover, he stated, the initial wave of Virginia settlers were "cavalier" gentlemen, which came from proud "armigerous families", followed Royalist dogma, devout in their Anglican faith, and held onto rural biases. Additionally, Fischer claimed, the rest of the early Virginia settlers were "a degraded rural proletariat who had no hope of rising to the top of their society" (787).
He also mentioned Americans have less than 20 percent of British heritage, yet the initial "folkways are constantly in process of creation, even in our own time." Thus, indicating these original mores have morphed over the years and still influence the social and business practices of this country (8). Furthermore, despite the small number of Americans with British heritage, Fischer points out how these morphed folkways are found in our language, i.e., how "New Englanders omitted h after w, so that whale became wale", or that Harvard became Haa-v'd (60).
Likewise, Fischer reported, after the war for independence these established preeminent Anglo-American groups rapidly grew, and by the nineteenth century "overspread the nation" (p. 832). Moreover, he added, these varied clans were "highly complex, involving differences of British region, religion, rank, and generation, as well as of the American environment" (788), which in turn, continued to shape our budding country.
Moreover, based on the complexity of these folkways, Fischer decided to examine and analyze American leadership. He looked at Franklin Roosevelt, George C. Patton, and Dwight Eisenhower, exploring how their leadership decisions were based on the differing folkways in which they were raised. Fischer claimed, Franklin Roosevelt owed much of his leadership practices to the folkways of New England. General George C. Patton and Dwight Eisenhower made their military decisions based on the varied folkways in which they were raised. Patton was influenced by his families ties to the Appalachian backcountry and Southern California, while General Eisenhower, who "hated fighting" was influenced by his Swiss Mennonite and German Pietist heritage who had settled in Quaker Pennsylvania (879).
Furthermore, Fischer indicated these regional mores, based on tradition, party loyalty, class, and ethnicity, helped shaped social, cultural, and political decisions made during the Civil War, Reconstruction, the world wars, as well as many other local, state, and national decisions. It also helped form political parties, such as The Progressive movement, which was comprised mostly of "Yankee stock, who traced their ancestry to the Puritan great migration"(867).
In Albion's Seed, the empirical evidence presented is organized around folkways and as Fischer indicated, folkways are complex with "many interlocking parts" (p. 7). He firmly demonstrated how these initial four folkways spread out across time and space in this country and how they provide an empirical way of measuring the varied peoples, the regions in which they live, and how their differing mores shaped and changed this country. Fischer's thesis is strong and compelling, with tangible evidence that will be hard to dismiss, thus this book is capable of changing our perception on the founding effects that built this country.
Featured image if from the books cover
Divide War
The Tangled Web Of Civil War Medicine
The Natural Ice Trade, preview video for my May 15th presentation at the Raney House in Apalachicola Florida
Critical review: Wood, Gordon S., The Creation of the American Republic, 1776-1787.
Civil War Medicine
HIST_701
Ice Trade
Revolutionary War Era
What Does It Take To Be An Entrepreneur?
2019 © Kevin Bair


What does it mean to wake up and become more conscious?
Let me share some perspectives that should make it easier to understand the process of waking up.
From the cellular perspective, you can see yourself as an individual person interacting with other individuals. You're like a single cell in the larger body of humanity, which is comprised of billions of other people-cells.
For example, I could say that I'm a guy (a cell) who's dedicated to helping people (other cells) live more consciously. I may communicate with many people during my lifetime, but each person is a unique individual, so the impact is different for everyone. We may all be part of some larger body of humanity, but our interactions mainly occur at the individual cellular level.
This is similar to one of the cells in your body noticing the other cells around it and deciding to do what it can to be of service to those cells. It may help a lot of cells, but it still regards itself as an individual cell helping other individual cells. And it won't help all cells equally, nor could it do so even if it tried.
From the holistic perspective, you see yourself as an integral part of the universe as a whole. The overall intent is to help universal consciousness grow and evolve, particularly the human consciousness of which you're a part.
This would be like one of the cells in your body recognizing that it's part of a larger physical body, whereby it stops thinking of itself primarily as an individual cell and begins to see itself as being of potential service to the greater whole. Its fate isn't as important as the fate of the larger body.
So with this perspective, instead of thinking of myself as a guy who helps people live more consciously, I can see myself as a servant of humanity helping to create a more conscious humanity, or as a servant of universal consciousness itself. My primary role here is to serve conscious evolution, which isn't necessarily what's best for any particular individual human in the short term.
Of course there are other perspective too. We could discuss identification with community, nation, all life, the cosmos, etc. These perspectives are equally valid, but exploring them would add complexity without adding much substance to the core ideas. So for now I want to keep this simple.
On the atomic side, you're an individual, and other people are individuals too. On the holistic side, we're all part of a greater whole.
I'm not suggesting that any one perspective is best. All of these perspectives are valid. But I will suggest that it's important to integrate the holistic perspective more fully into your life if you wish to experience a healthier flow of abundance.
"Waking up" basically means that you consider and integrate the holistic perspective as part of your daily life. Of course there are degrees of waking up, depending on how aware you are of the holistic perspective and how fully you've integrated it into your life. In the same manner, the cells in your body may have varying degrees of awareness that they are in fact part of a larger human body.
Alternatively, to be "asleep" is to be unaware of the larger holistic perspective. We could also define this behaviorally by saying that someone is asleep if they're aware of the holistic perspective, but they don't attempt to act congruently with it. In terms of semantics, I'd say that the first group is asleep, while the second group is trying to sleep.


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Q: grid-template-columns for IE11? So I have this website that runs fine on chrome, but not on IE11. I checked which CSS he's not receiving in IE11 and I see that once I turn off the: grid-template-columns in my chrome website, it does the same as in IE11. It doesn't put the elements next to eachother anymore because the width on the columns is set on 100% from an external file. I guess that could be changed but I'm wondering if there's a way I could get this behaviour to work in IE11? The code is:
grid-template-columns: repeat(3,1fr);
I have found that it indeed is not supported by IE11:
https://developer.mozilla.org/en-US/docs/Web/CSS/grid-template-columns

A: try
 -ms-grid-columns: ( 1fr 1em )[3];

or
-ms-grid-columns:1fr 1em 1fr 1em 1fr;

and for each grid items
-ms-grid-column:1;  -ms-grid-column:3; -ms-grid-column:5;


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Mobile Apps are components in the FleetAtlas framework. See where eLOG resides in the larger automation solution.
Get additional details on eLOG features, product screenshots, and eLOG's role in the larger automation & mobility framework.


In the midst of that newborn baby haze, an utterly exhausting but magical time, sometimes, just sometimes, you want to feel like yourself again, feel confident and pulled together. At Indigo Fig we've been there, and we get it.
That's why we've brought you a collection of Autumn and Winter 2017's freshest and most covet-worthy pieces that just so happen to be your postpartum body's best friend. Don't mention it.
Go for relaxed fit or swing style tops and dresses, peplum hems, drawstring waists, tie fronts and plenty of good old-fashioned streeetch.
For breastfeeding-friendly picks, see our Nursing collection.


Fascinating! This vibrant quilt is sure to brighten any room in your home! A twist on the Bargello pattern gives a stained glass look. Piecework is very well done. Hand quilted by a local Amish woman in shades of teal, brown and tan with black. Made here in Lancaster County.


Bringing you an old school arcade slot that goes by the name of Blue Diamond. A very simple game to understand with the feel of the Vegas slots, Blue Diamond Slot offers the chance to win 888x your stake by landing those precious gems.
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As we end January, we end 'Poverty Awareness Month'. Unfortunately, we won't see poverty end on January 31st. I guess we just won't need to be aware of it anymore. We'll go on to some other 'awareness' month that will also end without the issue ending. It's our way of making ourselves feel good; let's shine a light on an issue, hope it goes away in 30 days, and move on. Cynical maybe, but not far off the truth. It's the same way we make a big deal about the 'giving season' and shower organizations with money from November through December; and then ignore the need the other 10 months of the year.
Poverty in America is going to be with us on February 1st, make no mistake about that. Approximately 13.4% of the US population lives in poverty. That's over 43 million people. 15 million of them are children and 1 in 7 seniors are living in poverty. Those are numbers that demand more than 'awareness', they demand action.
What is so terrible about poverty is that it is more than a condition or a state of being. Poverty is an infection that destroys from without and from within. It creates hunger in children's lives, it destroys health, it reduces productivity, it increases crime, it drives addiction and self-destructive behavior, it causes abuse, it ruins lives, and it creating anxiety, hopelessness, and pain. And poverty is viral in that it feeds upon itself to create more poverty over generation.
Make no mistake, there is no single social condition more widespread or more dangerous to the future of a society than poverty. Poverty destroys communities and families, and when they are destroyed so is the fabric of a nation.
So as we end 'Poverty Awareness Month' let's commit to not end the fight against poverty. Let's commit to taking action. Let's commit to working together to create integrated and coordinated actions, programs, policies, and solutions that will ensure all aspects of the barriers to stability and self-sufficiency are looked at. It makes no sense to create job opportunities if there is not a transportation infrastructure that people can use to get to those jobs. Building shelters for the homeless is a good thing; a better thing to do is address and remedy the causes of homelessness and eviction so people don't become homeless. Hunger needs to be addressed, but food insecurity is the real challenge to permanently end systemic hunger.
The issues are complex, the solutions elusive. Let's not let ourselves be deceived that focusing on the issue for a few weeks and then moving on is going to accomplish anything. It won't.