Alma ordains priests and elders--He allows whoever repents and is baptized into the church--He expelled the proud and unrepentant--Alma goes to preach in the city of Gideon.
After Alma finished his speech, he reorganized the church a little bit. He ordained priests and elders, and allowed church membership to anyone who wasn't yet in the church, so long as they repented of their sins and were baptized.
6:2 aAnd it came to pass that whosoever did not belong to the church who repented of their sins were baptized unto repentance, and were received into the church.
6:3 aAnd it also came to pass that whosoever did belong to the church that did not repent of their wickedness and humble themselves before God -- I mean those who were lifted up in the pride of their hearts -- the same were rejected, and their names were blotted out, that their names were not numbered among those of the righteous. (6:3) I mean those who were lifted up.
But he also kicked out church members who didn't repent of their sins (especially the prideful ones!).
6:4 And thus they began to establish the order of the church in the city of Zarahemla.
6:5 Now I would that ye should understand that the word of God was liberal unto all, that none were deprived of the privilege of assembling themselves together to hear the word of God.
Alma told everyone to be good, and pray often, because he was leaving to go preach somewhere else. He was heading over to the newly built city of Gideon. (It was named after the Gideon guy who was killed by Nehor. The Book of Mormon explains it succinctly in verse 7).
(6:7) "Alma ... went ... into the valley of Gideon, there having been a city built, which was called the city of Gideon, which was in the valley that was called Gideon, being called after the man who was slain by the hand of Nehor with the sword."
6:7 aAnd now it came to pass that when Alma had made these regulations he departed from them, yea, from the church which was in the city of Zarahemla, and went over upon the east of the river Sidon, into the valley of Gideon, there having been a city built, which was called the city of Gideon, which was in the valley that was called Gideon, being called after the man who was slain by the hand of Nehor with the sword.
6:8 And Alma went and began to declare the word of God unto the church which was established in the valley of Gideon, according to the revelation of the truth of the word which had been spoken by his fathers, and according to the spirit of prophecy which was in him, according to the testimony of Jesus Christ, the Son of God, who should come to redeem his people from their sins, and the holy order by which he was called. And thus it is written. Amen. (6:8) And thus it is written. Amen.
So Alma went and preached in Gideon.


The elite divisions of the All-Ireland League, 1A and 1B, begin with the meeting of three-time and defending champions Lansdowne who travel to Castle Avenue to face a Clontarf team that won the title in 2014 and 2016 in a self-styled "Friday Night Lights" clash (8.0).
Shannon, who lead the title count with nine outright victories, are back among their peers following a five-year hiatus, after they claimed the Division 1B crown last season.
It is supposed to be the final season under the present framework but quite what restructuring will be agreed between the clubs and the IRFU going forward is not obvious at this point.
There is a big gap between what some of the clubs prefer and an IRFU model that is based on geographical inclusivity at elite level with no relegation for two years, while maintaining an outline commitment to ensure that the upper echelons of the All-Ireland Leagues (AIL) are not only a pathway for aspiring professional rugby players but an environment where they would regularly compete when not required by their respective provinces.
The clubs rejected a union proposal that the current limit of four professional players – two forwards, two backs – be increased to eight (four and four) with a compromise of six players, also rejected. These player proposals only apply in Divisions 1A and 1B. The participation agreements are due to be returned to the IRFU this week.
It's against this backdrop or impasse that it's worth examining the participation level of professional players in the 2017-2018 All-Ireland League – specifically in Divisions 1A and 1B. The parameters that govern the statistics are based on the 30 starting places in the 10 games across the two divisions on each weekend across the 18-match regular season.
There were 91 'fully contracted player starts' (FCP) which represents a little less than two per cent of the overall player total across the season in question. That number increased to 146 'development contracted player starts' (DCP) or three per cent and in terms of the 'academy contracted player starts' (ACP) that figure jumped to 552 and represents just under 10 per cent of the total player starts.
The FCP group, as the graphic outlines, those 91 starts have been accumulated by just 27 players and to delve slightly deeper it's germane to highlight the number of clubs by province that compete in Divisions 1A and 1B.
So the six players in Connacht all came from the Buccaneers club, the province's only representative in the AIL at that level. Leinster were represented by nine clubs, Munster by seven and Ulster by three. This season there will be no Ulster or Connacht clubs in Division 1A.
In the FCP category, Munster clubs fielded the most players (10) but across the Ulster clubs, despite two fewer players (eight) they racked up 42 starts. In terms of players on development contracts (DCP) Ulster clubs led the way in terms of number of players (10) and also starts (102) in contrast to the single Leinster player who started three matches.
There is no argument that the principal beneficiaries, evident in the figures compiled, are those on academy contracts (ACP) in the four provinces.
Whereas there were 27 fully contracted players used in the AIL last season and 19 on development deals, that number jumped to 74 when listing the involvement of academy players and they accounted for 552 starts.
This means that the predominant category of professionally contracted players in the All-Ireland League last season was largely aged between 18 and 21 years.
The league represents a significant forum for the development of young players and that's is why the IRFU is keen that a minimum of two clubs from all four provinces are represented in two divisions of eight teams – no relegation for two years – so that there is a greater tie-up between the elite end of the league and professional rugby.
That's certainly not the clubs' priority, nor should it necessarily be. But a central tenet in their argument for limiting the number of professionally contracted players that are eligible to play in a match, centring on health and safety in pitting amateurs against professionals is less persuasive given the figures.
Most clubs have two training sessions a week and players have their own gym/strength & conditioning regimes that are professional in orientation.
The IRFU want the elite divisions to be an adjunct to professional rugby, the clubs don't want to give up control.
If there is no compromise then the union will look to find game-time elsewhere for the professionally contracted players in an inferior competition from which neither side will benefit but ultimately it is the professionally contracted players that are set to lose most.


text:
Hebardina agaboides är en kackerlacksart som först beskrevs av Gerstaecker 1883.  Hebardina agaboides ingår i släktet Hebardina och familjen storkackerlackor. Inga underarter finns listade i Catalogue of Life.

Källor

Storkackerlackor
agaboides

redpajama_set_name: RedPajamaWikipedia


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text:
<tool id="qiime_pick_otus" name="Perform OTU picking" version="@WRAPPER_VERSION@.0">
    <description> (pick_otus)</description>
    <macros>
        <import>macros.xml</import>
    </macros>
    <expand macro="requirements">
        <requirement type="package" version="2.1b">sortmerna</requirement>
        <!--<requirement type="package" version="1.38.1.1">mothur</requirement>-->
        <!--<requirement type="package" version="2.2.22">blast-legacy</requirement>-->
        <requirement type="package" version="4.6.6">cd-hit</requirement>
    </expand>
    <version_command>pick_otus.py --version</version_command>
    <command detect_errors="aggressive"><![CDATA[
pick_otus.py
    --input_seqs_filepath '$input_seqs_filepath'
    --output_dir pick_otus
    --otu_picking_method '$method.otu_picking_method'

    #if $method.otu_picking_method == "sortmerna"
        #if $method.references.source_selector == 'history'
            --refseqs_fp '$method.references.refseqs_fp'
        #else if $method.references.source_selector == 'cached'
            --refseqs_fp '$method.references.refseqs_fp.fields.path'
        #end if
        --sortmerna_e_value '$method.sortmerna_e_value'
        --sortmerna_coverage '$method.sortmerna_coverage'
        #if $method.sortmerna_tabular.test == 'yes'
            --sortmerna_tabular
            --sortmerna_best_N_alignments '$method.sortmerna_tabular.sortmerna_best_N_alignments'
        #end if
        --sortmerna_max_pos '$method.sortmerna_max_pos'
        --similarity '$method.similarity'
        $method.suppress_prefilter_exact_match
        --threads \${GALAXY_SLOTS:-2}
    #else if $method.otu_picking_method == "mothur"
        --clustering_algorithm '$method.clustering_algorithm'
    #else if $method.otu_picking_method == "trie"
        $method.trie_reverse_seqs
    #else if $method.otu_picking_method == "uclust_ref"
        #if $method.references.source_selector == 'history'
            --refseqs_fp '$method.references.refseqs_fp'
        #else if $method.references.source_selector == 'cached'
            --refseqs_fp '$method.references.refseqs_fp.fields.path'
        #end if
        --similarity '$method.similarity'
        $method.enable_rev_strand_match
        $method.suppress_presort_by_abundance_uclust
        $method.suppress_new_clusters
        --max_accepts '$method.max_accepts'
        --max_rejects '$method.max_rejects'
        --stepwords '$method.stepwords'
        --word_length '$method.word_length'
    #else if $method.otu_picking_method == "blast"
        #if $method.references.source_selector == 'history'
            --refseqs_fp '$method.references.refseqs_fp'
        #else if $method.references.source_selector == 'cached'
            --refseqs_fp '$method.references.refseqs_fp.fields.path'
        #end if
        --max_e_value_blast '$method.max_e_value_blast'
        --min_aligned_percent '$method.min_aligned_percent'
        --similarity '$method.similarity'
    #else if $method.otu_picking_method == "sumaclust"
        --similarity '$method.similarity'
        '$method.sumaclust_exact'
        --denovo_otu_id_prefix '$method.denovo_otu_id_prefix'
        $method.suppress_prefilter_exact_match
        --threads \${GALAXY_SLOTS:-2}
    #else if $method.otu_picking_method == "swarm"
        --denovo_otu_id_prefix '$method.denovo_otu_id_prefix'
        --swarm_resolution '$method.swarm_resolution'
        --threads \${GALAXY_SLOTS:-2}
    #else if $method.otu_picking_method == "prefix_suffix"
        --prefix_length '$method.prefix_length'
        --suffix_length '$method.suffix_length'
    #else if $method.otu_picking_method == "cdhit"
        --similarity '$method.similarity'
    #else if $method.otu_picking_method == "uclust"
        --similarity '$method.similarity'
        --denovo_otu_id_prefix '$method.denovo_otu_id_prefix'
        $method.enable_rev_strand_match
        $method.suppress_presort_by_abundance_uclust
        $method.optimal_uclust
        $method.exact_uclust
        $method.user_sort
        --max_accepts '$method.max_accepts'
        --max_rejects '$method.max_rejects'
        --stepwords '$method.stepwords'
        --word_length '$method.word_length'
        $method.suppress_uclust_stable_sort
        $method.suppress_prefilter_exact_match
    #end if

    #if str($prefix_prefilter_length) != ''
        --prefix_prefilter_length '$prefix_prefilter_length'
    #end if
    $trie_prefilter
    --non_chimeras_retention '$non_chimeras_retention'

#if $method.otu_picking_method == "sortmerna"
    &&
    rm pick_otus/sortmerna_otus.log
#end if
    ]]></command>
    <inputs>
        <param argument="--input_seqs_filepath" type="data" format="fasta" label="Input sequences file"/>
        <conditional name="method">
            <param argument="--otu_picking_method" type="select" label="Method for picking OTUs">
                <option value="sortmerna">sortmerna</option>
                <!--<option value="mothur">mothur (requires an input file of aligned sequences)</option>-->
                <option value="trie">trie</option>
                <option value="uclust_ref">uclust_ref</option>
                <!--<option value="blast">blast</option>-->
                <!--<option value="sumaclust">sumaclust</option>-->
                <option value="swarm">swarm</option>
                <option value="prefix_suffix">prefix_suffix</option>
                <!--<option value="cdhit">cdhit</option>-->
                <option value="uclust" selected="true">uclust</option>
            </param>
            <when value="sortmerna">
                <expand macro="pick_otus_reference_source"/>
                <param argument="--sortmerna_e_value" type="float" value="1" label="Max E-value when clustering"/>
                <param argument="--sortmerna_coverage" type="float" value="0.97" min="0" max="1" label="Mininum percent query coverage (of an alignment) to consider a hit"/>
                <conditional name="sortmerna_tabular">
                    <param argument="test" type="select" label="Output alignments in the Blast tabular format with two additional columns including the CIGAR string and the percent query coverage?">
                        <option value="yes">Yes</option>
                        <option value="no" selected="true">No</option>
                    </param>
                    <when value="yes">
                        <param argument="--sortmerna_best_N_alignments" type="integer" value="1" label="How many alignments per read will be written"/>
                    </when>
                    <when value="no"/>
                </conditional>
                <param argument="--sortmerna_max_pos" type="integer" value="10000" label="Maximum number of positions per seed to store in the indexed database"/>
                <expand macro="pick_otus_similarity"/>
                <expand macro="pick_otus_suppress_prefilter_exact_match"/>
            </when>
            <when value="mothur">
                <param argument="--clustering_algorithm" type="select" label="Clustering algorithm">
                    <option value="furthest" selected="true">furthest</option>
                    <option value="nearest">nearest</option>
                    <option value="average">average</option>
                </param>
            </when>
            <when value="trie">
                <param argument="--trie_reverse_seqs" type="boolean" truevalue="--trie_reverse_seqs" falsevalue="" checked="false" label="Reverse seqs before picking OTUs for suffix (rather than prefix) collapsing?"/>
            </when>
            <when value="uclust_ref">
                <expand macro="pick_otus_reference_source"/>
                <expand macro="pick_otus_similarity"/>
                <expand macro="pick_otus_enable_rev_strand_match"/>
                <expand macro="pick_otus_suppress_presort_by_abundance_uclust"/>
                <param argument="--suppress_new_clusters" type="boolean" truevalue="--suppress_new_clusters" falsevalue="" checked="false" label="Suppress creation of new clusters using seqs that don't match reference?"/>
                <expand macro="pick_otus_max"/>
                <expand macro="pick_otus_stepwords"/>
                <expand macro="pick_otus_word_length"/>
            </when>
            <when value="blast">
                <expand macro="pick_otus_reference_source"/>
                <expand macro="pick_otus_similarity"/>
                <param argument="--max_e_value_blast" type="float" value="1e-10" label="Max E-value when clustering"/>
                <param argument="--min_aligned_percent" type="float" value="0.5" min="0" max="1" label="Minimum percent of query sequence that can be aligned to consider a hit"/>
            </when>
            <when value="sumaclust">
                <expand macro="pick_otus_similarity"/>
                <param argument="--sumaclust_exact" type="boolean" truevalue="--sumaclust_exact" falsevalue="" checked="false" label="Assign a sequence to the best matching seed rather than the first matching seed passing the similarity threshold?"/>
                <param argument="--sumaclust_l" type="boolean" truevalue="--sumaclust_l" falsevalue="" checked="true" label="Reference sequence length if the shortest?"/>
                <expand macro="pick_otus_denovo_otu_id_prefix"/>
                <expand macro="pick_otus_suppress_prefilter_exact_match"/>
            </when>
            <when value="swarm">
                <expand macro="pick_otus_denovo_otu_id_prefix"/>
                <param argument="--swarm_resolution" type="integer" value="1" label="Maximum number of differences allowed between two amplicons" help="Two amplicons will be grouped if they have integer (or less) differences"/>
            </when>
            <when value="prefix_suffix">
                <param argument="--prefix_length" type="integer" value="50" label="Prefix length"/>
                <param argument="--suffix_length" type="integer" value="50" label="Suffix length"/>
            </when>
            <when value="cdhit">
                <expand macro="pick_otus_similarity"/>
            </when>
            <when value="uclust">
                <expand macro="pick_otus_similarity"/>
                <expand macro="pick_otus_denovo_otu_id_prefix"/>
                <expand macro="pick_otus_enable_rev_strand_match"/>
                <expand macro="pick_otus_suppress_presort_by_abundance_uclust"/>
                <param argument="--optimal_uclust" type="boolean" truevalue="--optimal_uclust" falsevalue="" checked="false" label="Pass the –optimal flag to uclust?"/>
                <param argument="--exact_uclust" type="boolean" truevalue="--exact_uclust" falsevalue="" checked="false" label="Pass the –exact flag to uclust?"/>
                <param argument="--user_sort" type="boolean" truevalue="--user_sort" falsevalue="" checked="false" label="Pass the -user_sort flag to uclust?"/>
                <expand macro="pick_otus_max"/>
                <expand macro="pick_otus_stepwords"/>
                <expand macro="pick_otus_word_length"/>
                <param argument="--suppress_uclust_stable_sort" type="boolean" truevalue="--suppress_uclust_stable_sort" falsevalue="" checked="false" label="Don't pass –stable-sort to uclust?"/>
                <expand macro="pick_otus_suppress_prefilter_exact_match"/>
            </when>
        </conditional>
        <param argument="--prefix_prefilter_length" type="integer" label="Threshold to automatically group first identical prefix_prefilter_length into a single OTU" help="This is useful for large sequence collections where OTU picking doesn't scale well" optional="true"/>
        <param argument="--trie_prefilter" type="boolean" truevalue="--trie_prefilter" falsevalue="" checked="false" label="Prefilter data so seqs which are identical prefixes of a longer seq are automatically grouped into a single OTU?" help="This is useful for large sequence collections where OTU picking doesn't scale well"/>
        <param argument="--non_chimeras_retention" type="select" label="Selects subsets of sequences detected as non-chimeras to retain after de novo and reference based chimera detection">
            <option value="intersection">Intersection (retains only those sequences that are flagged as non-chimeras from both detection methods)</option>
            <option value="union" selected="true">Union (retains sequences that are flagged as non-chimeric from either filter)</option>
        </param>
    </inputs>
    <outputs>
        <data name="otus" format="txt" from_work_dir="pick_otus/*_otus.txt" label="${tool.name} on ${on_string}: OTUs"/>
        <data name="log" format="txt" from_work_dir="pick_otus/*_otus.log" label="${tool.name} on ${on_string}: Log"/>
        <data name="failures" format="txt" from_work_dir="pick_otus/*_failures.txt" label="${tool.name} on ${on_string}: Failures">
            <filter>method['otu_picking_method'] == "sortmerna" or method['otu_picking_method'] == "uclust_ref"</filter>
        </data>
        <data name="blast_output" format="tabular" from_work_dir="pick_otus/sortmerna_otus.blast" label="${tool.name} on ${on_string}: SortMeRNA Blast output">
            <filter>method['otu_picking_method'] == "sortmerna" and method["sortmerna_tabular"]["test"] == "yes"</filter>
        </data>
    </outputs>
    <tests>
        <test>
            <param name="input_seqs_filepath" value="pick_otus/seqs.fna"/>
            <param name="otu_picking_method" value="uclust"/>
            <param name="similarity" value="0.97" />
            <param name="denovo_otu_id_prefix" value="denovo"/>
            <param name="enable_rev_strand_match" value=""/>
            <param name="suppress_presort_by_abundance_uclust" value=""/>
            <param name="optimal_uclust" value=""/>
            <param name="exact_uclust" value=""/>
            <param name="user_sort" value=""/>
            <param name="max_accepts" value="1"/>
            <param name="max_rejects" value="8"/>
            <param name="stepwords" value="8"/>
            <param name="word_length" value="8"/>
            <param name="suppress_uclust_stable_sort" value=""/>
            <param name="suppress_prefilter_exact_match" value=""/>
            <param name="trie_prefilter" value=""/>
            <param name="non_chimeras_retention" value="union"/>
            <output name="otus" md5="2ca01ee393e8a795e5d09a15c5ca77c3"/>
            <output name="log">
                <assert_contents>
                    <has_text text="UclustOtuPicker parameters"/>
                </assert_contents>
            </output>
        </test>
        <test>
            <param name="input_seqs_filepath" value="pick_otus/seqs.fna"/>
            <param name="otu_picking_method" value="sortmerna"/>
            <param name="source_selector" value="history"/>
            <param name="refseqs_fp" value="pick_otus/refseqs.fasta"/>
            <param name="sortmerna_e_value" value="1"/>
            <param name="sortmerna_coverage" value="0.97"/>
            <param name="test" value="yes"/>
            <param name="sortmerna_best_N_alignments" value="1"/>
            <param name="sortmerna_max_pos" value="10000"/>
            <param name="similarity" value="0.97"/>
            <param name="suppress_prefilter_exact_match" value=""/>
            <param name="trie_prefilter" value=""/>
            <param name="non_chimeras_retention" value="union"/>
            <output name="otus" md5="dbc3e13e7da742ac3df317054821f522"/>
            <output name="log">
                <assert_contents>
                    <has_text text="OtuPicker parameters"/>
                    <has_text text="SortMeRNA database"/>
                </assert_contents>
            </output>
            <output name="failures" md5="cce9699de546618df2db9de7a8098916"/>
            <output name="blast_output" md5="214b1674f5f84dcb68c0c0c1c8a001be"/>
        </test>
        <test>
            <param name="input_seqs_filepath" value="pick_otus/seqs.fna"/>
            <param name="otu_picking_method" value="trie"/>
            <param name="trie_reverse_seqs" value=""/>
            <param name="trie_prefilter" value=""/>
            <param name="non_chimeras_retention" value="union"/>
            <output name="otus" md5="441c2bc34cd6766e75585dbe2ea09f9b"/>
            <output name="log">
                <assert_contents>
                    <has_text text="TrieOtuPicker parameters:"/>
                </assert_contents>
            </output>
        </test>
        <test>
            <param name="input_seqs_filepath" value="pick_otus/seqs.fna"/>
            <param name="otu_picking_method" value="uclust_ref"/>
            <param name="source_selector" value="history"/>
            <param name="refseqs_fp" value="pick_otus/refseqs.fasta"/>
            <param name="similarity" value="0.97"/>
            <param name="enable_rev_strand_match" value=""/>
            <param name="suppress_presort_by_abundance_uclust" value=""/>
            <param name="suppress_new_clusters" value="" />
            <param name="max_accepts" value="1"/>
            <param name="max_rejects" value="8"/>
            <param name="stepwords" value="8"/>
            <param name="word_length" value="8"/>
            <param name="trie_prefilter" value=""/>
            <param name="non_chimeras_retention" value="union"/>
            <output name="otus" md5="e44b21022227e9fc213e13fd028efb81"/>
            <output name="log">
                <assert_contents>
                    <has_text text="OtuPicker parameters"/>
                    <has_text text="Application:uclust"/>
                </assert_contents>
            </output>
            <output name="failures" md5="d41d8cd98f00b204e9800998ecf8427e"/>
        </test>
        <test>
            <param name="input_seqs_filepath" value="pick_otus/seqs.fna"/>
            <param name="otu_picking_method" value="swarm"/>
            <param name="denovo_otu_id_prefix" value="denovo"/>
            <param name="swarm_resolution" value="1"/>
            <param name="trie_prefilter" value=""/>
            <param name="non_chimeras_retention" value="union"/>
            <output name="otus" md5="9b603183a1116071e762e31525928f94"/>
            <output name="log">
                <assert_contents>
                    <has_text text="OtuPicker parameters"/>
                </assert_contents>
            </output>
        </test>
        <test>
            <param name="input_seqs_filepath" value="pick_otus/seqs.fna"/>
            <param name="otu_picking_method" value="prefix_suffix"/>
            <param name="prefix_length" value="50"/>
            <param name="suffix_length" value="50"/>
            <param name="trie_prefilter" value=""/>
            <param name="non_chimeras_retention" value="union"/>
            <output name="otus" md5="3bf79c70affb264a977fd8f2f34f2889"/>
            <output name="log">
                <assert_contents>
                    <has_text text="PrefixSuffixOtuPicker parameters:"/>
                </assert_contents>
            </output>
        </test>
        <test>
            <param name="input_seqs_filepath" value="pick_otus/seqs.fna"/>
            <param name="otu_picking_method" value="uclust"/>
            <param name="similarity" value="0.97"/>
            <param name="denovo_otu_id_prefix" value="denovo"/>
            <param name="enable_rev_strand_match" value=""/>
            <param name="suppress_presort_by_abundance_uclust" value=""/>
            <param name="optimal_uclust" value=""/>
            <param name="exact_uclust" value=""/>
            <param name="user_sort" value=""/>
            <param name="max_accepts" value="1"/>
            <param name="max_rejects" value="8"/>
            <param name="stepwords" value="8"/>
            <param name="word_length" value="8"/>
            <param name="suppress_uclust_stable_sort" value=""/>
            <param name="suppress_prefilter_exact_match" value=""/>
            <param name="trie_prefilter" value=""/>
            <param name="non_chimeras_retention" value="union"/>
            <output name="otus" md5="2ca01ee393e8a795e5d09a15c5ca77c3"/>
            <output name="log">
                <assert_contents>
                    <has_text text="UclustOtuPicker parameters"/>
                </assert_contents>
            </output>
        </test>
    </tests>
    <help><![CDATA[
**What it does**

The OTU picking step assigns similar sequences to operational taxonomic units, or OTUs, by clustering sequences based on a user-defined similarity threshold. Sequences which are similar at or above the threshold level are taken to represent the presence of a taxonomic unit (e.g., a genus, when the similarity threshold is set at 0.94) in the sequence collection.

Currently, the following clustering methods have been implemented in QIIME:

1.  uclust, creates "seeds" of sequences which generate clusters based on percent identity.

2.  uclust_ref, as uclust, but takes a reference database to use as seeds.  New clusters can be toggled on or off.

3.  usearch, creates "seeds" of sequences which generate clusters based on percent identity, filters low abundance clusters, performs de novo and reference based chimera detection.

4.  usearch_ref, as usearch, but takes a reference database to use as seeds.  New clusters can be toggled on or off.

5. sumaclust, creates "seeds" of sequences which generate clusters based on similarity threshold.

6. swarm, creates "seeds" of sequences which generate clusters based on a resolution threshold.

Chimera checking with usearch 6.X is implemented in identify_chimeric_seqs.py.  Chimera checking should be done first with usearch 6.X, and the filtered resulting fasta file can then be clustered.

The primary inputs for pick_otus.py are:

1. A FASTA file containing sequences to be clustered

2. An OTU threshold (default is 0.97, roughly corresponding to species-level OTUs);

3. The method to be applied for clustering sequences into OTUs.

pick_otus.py takes a standard fasta file as input.

The output consists of two files (i.e. seqs_otus.txt and seqs_otus.log). The .txt file is composed of tab-delimited lines, where the first field on each line corresponds to an (arbitrary) cluster identifier, and the remaining fields correspond to sequence identifiers assigned to that cluster. Sequence identifiers correspond to those provided in the input FASTA file.  Usearch (i.e. usearch quality filter) can additionally have log files for each intermediate call to usearch.

Example lines from the resulting .txt file:

=   ====    ====    ====
0   seq1    seq5
1   seq2
2   seq3
3   seq4    seq6    seq7
=   ====    ====    ====

This result implies that four clusters were created based on 7 input sequences.
The first cluster (cluster id 0) contains two sequences, sequence ids seq1 and
seq5; the second cluster (cluster id 1) contains one sequence, sequence id seq2;
the third cluster (cluster id 2) contains one sequence, sequence id seq3, and the
final cluster (cluster id 3) contains three sequences, sequence ids seq4, seq6,
and seq7.

The resulting .log file contains a list of parameters passed to the pick_otus.py
script along with the output location of the resulting .txt file.
    ]]></help>
    <citations>
        <expand macro="citations"/>
        <citation type="doi">10.1093/bioinformatics/btv231</citation>
        <citation type="doi">10.1093/bioinformatics/btq461</citation>
        <citation type="doi">10.1093/bioinformatics/bts611</citation>
        <citation type="doi">10.7287/peerj.preprints.386v1/supp-1</citation>
    </citations>
</tool>

redpajama_set_name: RedPajamaGithub


To my daddy. I love you and I wish I could be with you today.
If you love your dad, tell him today. If you get a chance, give him a hug. If he's at an Indy car race in Milwaukee, call him after the checkered flag.
And don't forget your baby daddy. I love you, my Beloved Spouse. Thanks for being such a great dad to our Precocious Daughter.
The unbreakable bond between father and daughter.
Sorry if that sounds like a threat.
Happy Father's Day to all dads and their kids!


text:
South Coast-North
South Coast-South
Tourney Brackets
CCCWBCA
Coaches vs. Cancer
'20 Sophomore Showcase
Soph Showcase Registration
Herrick hits 600 career wins, College of the Canyons downs Rio Hondo, 78-67
Rio Hondo (7-6) 13 16 8 18 55
Glendale (10-3) 16 13 17 16 62
College of the Canyons head coach Greg Herrick won his 600th career game recently. (Jesse Munoz, COC Athletics)
Pts: Jennyfer Morales - 29
Reb: Jennyfer Morales - 8
Ast: 2 Players (#2, #20) - 3
Pts: Kayla Wrobel - 23
Reb: 2 Players (#11, #20) - 8
Ast: Vicky Oganyan - 4
Pts off Turnovers
2nd Chance Pts
Pts in the Paint
Fastbreak Pts
Bench Pts
GLENDALE — College of the Canyons head coach Greg Herrick picked up his 600th career victory at the college on Friday, as the Cougars fought for a historic 78-67 win over Rio Hondo College during day one action of the Glendale Crossover.
The victory, which by virtue of schedule, came nearly two weeks after Herrick claimed career win No. 599 prompted an emotional post-game celebration from the group of friends, family, colleagues, fans and supporters who were in attendance.
"This acknowledges all of the players over the course of the last 29 seasons that contributed to the 600 wins," said Herrick, moments after being presented with the official game ball and having an opportunity to address the crowd.
"I get the credit, but the team, and all the players are the ones that should get all the credit because they're the ones that did it," added Herrick, who took over the COC women's program prior to the 1992-93 season.
"I'm very happy, I'm proud of what we've done all these years, but it's all about the players," he said. "With all the players that I've coached, there is a love affair with all of them. If they were all here, I'd thank every one of them."
Herrick's 30-year tenure at College of the Canyons has included 16 Western State Conference (WSC), South Division Championships (1994, 1995, 1997, 1998, 2000, 2001, 2004, 2006, 2007, 2008, 2009, 2010, 2011, 2012, 2016 and 2018) in 29 seasons. COC did not compete during the 2020-21 year due to cancellations brought on by the COVID-19 pandemic.
His teams have also qualified for the CCCAA Southern California Regional Postseason in 23 seasons, including a run of 21 straight appearances from 1994-2014. In 1997, Herrick guided the Cougars all the way to the state championship game, eventually finishing runner-up to Ventura College.
Herrick has also been named the WSC, South Coach of the Year on 10 occasions.
As of Friday night, Herrick boasts a career record of 600-277 at the college, good for a .684 winning percentage.
Herrick's tenure at College of the Canyons has been one of the most successful of any head coach in CCCAA women's basketball history.
"College of the Canyons gave me the opportunity to coach the sport I love so much," said Herrick. "When somebody gives you that opportunity and gives you the freedom to build the program in your image, I am overly appreciative of that. I will never forget them for giving me this chance."
No. 4 state ranked Canyons (9-1) was able to bounce back for the win against Rio Hondo (5-8) after suffering its first loss to San Joaquin Delta College its last time out on Dec. 4. That matchup vs. No. 1 ranked Delta came in the championship game of the 45th Annual Gilcrest Invitational on Dec. 4.
On Friday, the two teams played to a 39-39 halftime tie as the Roadrunners attempted to take a page from the Cougars' playbook with a fast-paced offense and pressing defense.
Canyons took a slim 57-56 lead into the fourth quarter before creating some separation thanks in large point to seven points from Justice Walters across the final 10 minutes of play.
Walters came off the bench but finished with a game-high 25 points on an 8-of-19 shooting night that included four three-pointers. Her three-point basket with 5:19 to go in the game put COC ahead by eight points with the Roadrunners unable to get much closer.
"Rio Hondo gave us everything they had," said Herrick. "We didn't really get after it until the end."
COC freshman LuLu Salloom finished with 14 points and 16 rebounds. Sophomore Malia Semana was next with 13 points and seven rebounds. Danni Diaz and Monique Febles finished with 12 and 10 points, respectively.
Rio Hondo received 20 points from Rosalie Avalos and 15 from reserve Jiselle Cabrera to help keep pace with Canyons.
COC outrebounded the Roadrunners 41-27 and shot better than 70 percent (27-of-38) from the foul line. By comparison, Rio Hondo was 7-of-12 from the charity stripe.
The Cougars will return to action on Saturday, Dec. 18 vs. No. 13 state ranked Allan Hancock College in day-two action at the Glendale Crossover. That contest is scheduled to tip at 1 p.m.
Canyons is off to the best start in program history currently averaging 73 points per game to rank third in the WSC, South. Herrick and the Cougars appear poised to compete for another WSC title when conference play begins on Jan. 5.
"I told the players, we've always been the hunter, now we are the hunted," said Herrick. "Other teams want to beat us. We're highly ranked and have a pretty gaudy record.
"There are a lot of good teams out there," added Herrick, "but if things go our way, you've got to consider us having a chance, and maybe favorites, to take the conference."
(Jesse Munoz, College of the Canyons Athletics)

redpajama_set_name: RedPajamaCommonCrawl


text:
Today: All Hail Shane Smith & The Saints
Shane Smith & The Saints New Album, Hail Mary, Out Now
Austin, Texas (June 28, 2019) … Americana powerhouses Shane Smith & The Saints full album, Hail Mary, is available today. Released in a series of chapters to their eager fans, the final chapter, The End, is out now on all digital platforms. Chapter 4: The End features two new songs: "Last Train to Heaven" and "The End." Produced by Mark Needham (The Killers, Imagine Dragons), Hail Mary has been called the definitive album in the band's discography.
The band parsed the deeply-biographical project into four chapters: The Beginning, The Fall, The Path, and The End. Americana Highways described Hail Mary as "an anthemic saga through several stages, with Smith's soaring vocals and the band rallying their grit."
Grit is something Shane Smith & The Saints have in spades; Hail Mary didn't come easy to the members of the band. With nearly two-thirds of the year spent on the road, the band has seen and been through it all. These guys have survived and triumphed through three near-death experiences (a rattlesnake bite, a motorcycle accident, a pulmonary embolism while on tour in Malaysia) and relationship turbulence in their personal lives to create this stunning new record. Inspired by their journey, fans can experience from The Beginning to The End the band's trials, tribulations, and triumphs.
Each chapter has its own theme, and now, at its denouement, Hail Mary finishes with an emboldened flourish. Chapter 4: The End premiere partner The Boot hit the nail on the head, saying, "the traumatic events of Chapter 2 and the recovery detailed in Chapter 3 are in the past, and the final songs assess what is left and look optimistically toward the future."
Hail Mary is available now.
Shane Smith & The Saint's future is looking bright with a summer tour to support the new album.
Find out more at ShaneSmithMusic.com.
7/12 | Helotes, TX | Floore's Country Store
7/13 | Lampasas, TX | Spring Ho Festival
7/18 | Paris, TX | Heritage Hall
7/19 | The Colony, TX | Lava Cantina
7/20 | Littlefield, TX | Celebrate Littlefield
7/24 | Tucson, AZ | The Rock
7/25 | Las Vegas, NV | Vinyl
7/27 | Sacramento, CA | Goldfield Trading Post
7/30 | Seattle, WA | Tractor Tavern
8/1 | Ontario, OR | Malheur County Fairgrounds
8/2 | Joseph, OR | Harley Tucker Rodeo Grounds
8/3 | Hailey, ID | The Mint
8/8 | Fort Collins, CO | Aggie Theatre
8/9 | Denver, CO | Bluebird Theatre
8/10 | Boulder, CO | Boulder Theatre
8/14-15 | Tomball, TX | Main Street Crossing
8/16 | Corpus Christi, TX | Brewster Street Ice House
8/17 | Hutto, TX | KOKE Fest
8/23 | Odessa, TX | Dos Amigos
8/24 | Ropesville, TX | 6282 Music Fest
8/25 | Plano, TX | Love & War in TX
More on Shane Smith & The Saints:
From their home base in Austin to performances across the country and abroad, Shane Smith & the Saints have not just stuck stubbornly to their musical and lyrical convictions. They've defied audience expectations by delivering incendiary shows, each one ignited by the band's ability to unleash, feed on and send back the energy of the crowd.
"Take the Americana- and folk-esque instrumentation of a band such as the Avett Brothers and the harmonies of the Zac Brown Band, put them together with a Bruce Springsteen-like voice and the lyrical sensibilities of Bob Dylan, and then throw in a bit of Celtic rock a la the Dropkick Murphys, and you'll wind up with something along the lines of Shane Smith & the Saints." - The Boot
Kate Richardson
kate@richlynngroup.com
Garry Fitch June 28, 2019
THE DOOBIE BROTHERS: LIVE FROM THE BEACON THEATRE
2'Live Bre Continues the Fiesta


text:
Divorce Diva
Banks Warned Over Failure to Move Employees in Time for Brexit
Home /News/Banks Warned Over Failure to Move Employees in Time for Brexit
Banks are waiting until the last minute to move staff and resources to their new European Union divisions, risking disarray during a no-deal Brexit, according to Europe's top banking regulator.
With three weeks to go before Britain is due to leave the EU, banks have built the necessary infrastructure to handle the fallout but need to move employees more quickly, Jose Manuel Campa, chairman of the European Banking Authority, said in an interview on Tuesday.
"It's understandable on a human basis, but if you're concerned about financial instability, it's not the best thing you want to hear," he said.
The warning comes as senior financial executives sound alarms about the potential ramifications of a no-deal Brexit. Societe Generale SA Chairman Lorenzo Bini Smaghi called it a "systemic event" that could lead to a global recession. The pound dropped on Tuesday after a British official said a no-deal Brexit was likely because of an impasse in talks with German Chancellor Angela Merkel.
Financial regulators have spent months trying to ensure key parts of the financial industry can continue in a hard Brexit, and Campa said that work has helped to prepare the market. Still, one of the EBA's biggest concerns is "that there is operational continuity," he said. "The infrastructure is there, but it hasn't been tested fully. It's not been fully operational."
Problems could arise from simple logistical issues if staff moves are rushed, such as missing "authorizations to get into the building," according to Campa.
Typhoon Hagibis: Homeless men denied shelter in middle of typhoon
Harry and William deny 'offensive' bullying claims
Neil Peart: Rush drummer dies aged 67
Prince William speaks about mental health on Pakistan school visit


Guwahati is the largest city of Assam, a major riverine port city and one of the fastest growing cities in India. The gateway to the Northeast and the largest cosmopolitan city in the region. From the local cuisines to the beautiful ponds, palm trees, small traditional houses and so much more Guwahati has to and can offer. We provide the best services to our clients who are visiting North East to make their journey joyful and comfortable. We arrange their accomodation in some of the best hotels in these states with a touch of local tastes.
We are backed by an experienced team of professionals who design customised tour packages for each client ensuring maximum fun and leisure. Tucked away in the hills of eastern sub-Himalayas is Meghalaya, one of the most beautiful State in the country. Nature has blessed her with abundant rainfall, sun-shine, virgin forests, high plateaus, tumbling waterfalls, crystal clear rivers, meandering streamlets and above all with sturdy, intelligent and hospitable people.
After breakfast transfer to Bhalukpong & overnight.
After breakfast drive to Dirang. Enroute visit Tippi Orchid Centre. Check in Hotel. Evening at Leisure. Overnight at Dirang.
Morning drive to Tawang. Enroute witness the snowcapped Sella Pass at 14000 ft and the Jaswant Garh War Memorial. (Legend has it that Jaswant (Mahavir Chakra Awardee (Posthumous) of 4th Batallion Garhwal Rifles), fought a lone battle against the invading Chinese during the 1962 war. His spirit is still believed to protect the place). Overnight in Tawang.
Tawang - The beautiful land of the Monpa tribes with the "GALDEN NAMGYEL LHATSE" Monestary - one of the largest Lamaseries of Mahayana sect in Asia. The birth place of the 6th Dalai Lama, the Monastery is 400 year old and is adorned with the 18 feet high statue of Lord Buddha. 'The Land of Dawn - lit Mountains' at 3500 mts gives you unparallel purity of nature. Visit the monastery and the War Memorial. Evening free for shopping. Overnight in Tawang.
Morning drive back to Bomdila. Evening free at leisure. Overnight in Bomdila.
After breakfast drive to Kaziranga. Check in Hotel. Overnight in Kaziranga.
After breakfast drive to Guwahati. Afternoon visit Kamakhya temple. Evening River cruise over the river Brahmaputra. Overnight in Guwahati.
After breakfast transfer to Guwahati airport for onward journey. Tour Ends Here.


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Here's something a little different: Usually, before I write a World of Warcraft column (or any column), my assumptions and data are pretty firm before I put them down on paper, else I wouldn't be writing it in the first place. This is one of the reasons that, for example, I spent so much time showing my work when trying to predict the launch date for Battle for Azeroth; that was all about hard numbers, so it was easy to check math and assumptions in an obvious fashion.
But in this particular case I'm exploring a concept that I'm still playing with and researching, something that may turn out to be somewhat erroneous. To wit: I suspect World of Warcraft expansions have switched from selling to existing customers and into reclaiming old customers as a primary design focus.
It might seem like an odd assertion, but I think it's an interesting thing to consider and may help shed light on a number of design decisions, several of which I think are pretty bad ones. But for this particular column I'm not interested in analyzing the merits of design choices; I'm interested in presenting the evidence and showing how it lines up in a more neutral fashion. Because I think it can shape some interesting thinking.
First of all, let me make one thing clear: Beyond a certain point, any and all discussion of subscription numbers for WoW is purely speculative because Blizzard stopped giving out subscriber numbers. This is for obvious reasons because the game has remained profitable despite reduced numbers, and there's a whole lot of analysis to be done about that when the game doesn't really lean heavy into its cash shop. There's even a dearth of cosmetic armor there, which is usually a mainstay; new releases on the cash shop are rare and usually specific mounts or pets, often with charity donations. But the company has stopped believing that subscriber numbers are a good thing to release.
As part of a report delivered to investors to assure them that yes, all is well in terms of return on investment, this is a reasonable decision. As something analysts like to examine, this is horrible because it means guessing about the numbers beyond a certain point. Knowing the line of overall subscriptions through Legion would be super helpful, but we're guessing.
However, I think things work just fine with the line that we do have, and the lack of new subscriber numbers tends to indicate that the trend has remained consistent or at least did not reverse.
So let's start with something that everyone knows, which is also a matter of public record. From its launch in November of 2004 through the early launch days of Cataclysm in 2011, WoW literally never dropped its numbers. Every new announcement from the company was either maintaining the same numbers or announcing another new benchmark. It was only during Cataclysm that numbers actually started to drop.
This is not to say that no one unsubscribed from WoW during that time; speaking purely from anecdotal experience I know that I did at least two times, other people also did, and logically speaking you would assume some churn. The result is that it's hard to guess how many people have actually played the game in total over time, but the number is almost certainly higher than the game's peak of 12 million.
Why does the total matter? Because of market saturation.
Market saturation is essentially the point wherein your overall market share is just not going to grow any longer for anything. People know your product exists and either have chosen to buy it, have chosen not to buy it, or have stopped buying it. This is also the point where trying to catch new customers is no longer worth the effort, because you're chasing people who are just never going to buy in.
For assorted reasons, WoW didn't hit saturation until after the launch of Cataclysm, but it certainly does seem to have hit that point then. From that point onward, the game's numbers, or at least the ones we know about, have gone up and down, but they've never actually grown beyond 12 million. Again, it's almost certain the actual number of total players over time is higher, but we don't have access to that statistic.
Purely for argument's sake, let's say that there were exactly as many former players as current players at that time, which would make the market saturation point 24 million. This is, again, a totally arbitrary and speculative number; it could be 20 million, 100 million, or 12.5 million. It just places that at a good middle point.
The point is that by the time we start seeing drops in Cataclysm, we can make the assumption that WoW is a known quantity. Everyone who is a potential player is either currently playing or a former player. And as the numbers slide down, it seems like there's an obvious shift in focus for expansions, marketing less to the people already playing and more to the people who were playing and left.
Marketing for Cataclysm played this up a little, with the old world revamp and the exploration of places long visible but not accessible in the game. But it clearly wasn't a major focus because most of these things required knowledge of the game to begin with. You had to be pretty invested in the game to remember Uldum or Grim Batol; they were areas referenced but never seen in the base game. Improvements to the leveling zones made the experience smoother but were clearly touted as something to remove the tedium and design issues for people who had leveled through this many times before.
In Mists of Pandaria we also got a nod to the long-running issue of the Pandaren, who had been used as a background element in the franchise for ages without ever being strictly explored. Still, though, it seems that this expansion was far more marketed at people who were already playing rather than potential returning players.
But then we have Warlords of Draenor, which did two things to really push hard at players who used to play and left. The first was the inclusion of a very well-known region redone and re-imagined, along with a cast list that lots of players likely had dim memories of. You might not remember exactly what their deals were if you were never into the lore, but you know that, say, Gul'dan and Grom Hellscream were important. You know that Outland used to be Draenor. (And you possibly still call it "the Outlands" or "Outlands" or "Stevetown" or some other name that's completely wrong.) All of this stuff aligns nicely with fuzzy memories.
The other shift? The level boost. Not only can you hop back in with those fuzzy memories, you can do so without having to go through the process of catching up. The game will catch you up and drop you into the experience right away!
From a marketing perspective, this worked like a charm. Subscriptions jumped by nearly 3 million between the end of Mists and the launch of Warlords. People came back! Admittedly, the game's next month saw another subscription drop that was actually lower than the nadir of Mists, but from the standpoint of reclaiming people in that departing group? It worked, albeit temporarily.
Subsequently, Legion and Battle for Azeroth have both leaned in on this. Legion contained artifacts, several of which occupy that fuzzy memory space ("yeah, the Ashbringer was a big deal, right?"), and it also contains zones that seem to be functionally designed as WoW's Greatest Hits. People like Tauren, so here's a Tauren zone. People like druids, so here's the druid zone. Remember that whole storyline about Blood Elves withering away without magic? The Nightborne hit that same story beat-for-beat, without ever tying it to factional conflict (that would come later).
And Battle for Azeroth? Please note that while the Allied Races introduced as pre-order bonuses are races that you would be familiar with only if you'd been playing the game at the time, the ones coming with the expansion are right back into that fuzzy memory space. You remember Dark Iron Dwarves and Zandalari Trolls and Mag'har Orcs and something about Kul Tiras, right? You might not remember exactly what their deals were, but you can play them now and you get a level boost to catch right back up. Vague memories propel you back.
Whether or not this is a good thing is a discussion for another day, but I think this adds an extra dimension to discussion of standalone expansions and to understanding overall design decisions. There are a number of choices going on here that make perfect sense if you think about the developers are not trying to court new players or existing ones but trying to recapture players who did play at one point and are curious to come back. And letting them do so without having to catch up? There's a reason for that, too.
Feedback, of course, is welcome down below or via mail to eliot@massivelyop.com. There's a lot more analysis to be done here and discussion about where good and bad decisions come into play, but as stated the point here was to present the concept rather than dissect it. Putting everything into this framework explains a lot of decisions, even if it doesn't make them right.
Just look at the graphics of this more than 15 year old game: new players are totally put off by the look alone, it costs them a lot of overcoming to start a new game this ugly. Old players on the other hand have dealt with the look at least once and possibly don't mind, since they remember the game emotionally and got a foot in the door. So it's way easier to lure the veterans back than convince the spoiled new ones.
I'm not sure I agree that allied races are there to bring back people.. I think they're there to keep people. Right now someone can't pick up the game and make a Nightborne. They have to go grind a whole lot of reputation (that hasn't been nerfed) just to get the ability to make one… then if they want the armor and mount, they have to level it, not boost it. They introduced this at the end of legion, not at the beginning.
Come BFA the new allied races will be in the same boat. Get your BFA reps up to unlock them. So a new player can't just come back and make a Mag'har Orc. They need to come back, buy the expansion, grind the reputation, and then start a new character.
To me it seems like Allied races are about extending the life of the game for the existing subscribers, not draw new ones in.
I hate quoting myself, but….
And it's important to note as well that most of the things you need to do to unlock these races are, just like with the Legion-based races, things that you are already going to be doing if you're playing. It's not a matter of going out of your way to unlock them; it's a matter of continuing to do the same things you're already doing.
Remember that JG (studio head) said wildstar's target audience was former wow players. JG was ex wow and he said for mmos about 10% of mmo players leave every month and half don't return. which means half do. ( every four seconds a player leaves wow or teso) My guess is that the leaving and returning % have both increased.
Over 4 and a half years ago the wow info graphic was that they had over a hundred million accounts. Ergo, IMO, 124 M or 150 M is a better current estimate than 24m. So unrealistically if all current players left and ten % of former players returned, it would be a financial success.
During the silly wow is dieting days, ghostcrawler said the people leaving wow was not up much it was that returning players were down resulting in drop in current/net subs.
The cheapest customer to get is existing players, then former, then wow virgins, then mmo virgins.
Over 4 and a half years ago the wow info graphic was that they had over a hundred million accounts. Ergo, IMO, 124 M or 150 M is a better current estimate than 24m.
Hey, if you'd rather use that estimate, go nuts; it literally does not matter, like it says. I'm not using it for math, I'm using it to make a point.
Blizzards biggest issue has always been the 'we are too big to fail and the consumer is locked in, time to change focus for another catchment to add to the pile' not noticing a portion of the existing consumers become former consumers over time as new things appear to offer what Blizzard let slide 'because we already got them'. Not just in WoW either.
I agree with this piece. It's clear that Blizzard is trying to get the old players back by going back to the basics – war between Alliance and Horde. This is what elevated Warcraft from the very first game (albeit it was humans vs orcs), and it's what captivated a lot of the original players that were migrating from PVP-centric MMOs of the day.
I also think that it's a good thing. We've had plenty of expansions where we're fighting the big bad demons/old gods/corrupted whatever, and it's time to go back to what the game is all about – the conflict between very different civilizations.
What BfA will actually bring once it launches is to be seen. I'm sincerely hoping that we don't get yet another version of "it was me all along!" type deal with old gods or other influencing entities, and rather accept the fact that Alliance and Horde simply cannot coexist without conflict.
…and rather accept the fact that Alliance and Horde simply cannot coexist without conflict.
So long as there is hackneyed writing, there can never be peace.
In all seriousness, I don't think this is an inherently bad thing or a good thing; rather, it carries with it a certain set of accompanying decisions, and those decisions can be good or bad, but it helps to consider and pick apart the ethos behind those decisions. Hence why the point made here is that it's meant to be presented in a neutral tone.
Once you understand why these particular decisions are being made, you can start to examine whether or not these are worthwhile goals, whether or not the decisions made in that direction accomplish those goals, and whether or not there are better choices to be made that might catch some or all of the positives without the negatives.
It's certainly possible that was their goal but I'm not sure either way. I do know that I quit at some point after WoTLK and only hopped into the game very briefly here and there until Legion came out. I came back to the game last year and loved Legion as much or more than WoTLK. I think it's been one of their best expansions in recent years and so far BfA is looking promising, but obviously too early to tell until it's out.
So if their goal was to bring back old players, I think they eventually refined that well enough by the time Legion came out because it worked for me and it feels like a lot of other players are in the same boat.
And if there's any expac that's going to bring new players, the few that still exist, it seems to be BfA. I've seen a number of posts on Reddit and the Forums with brand new players getting into the game and asking for advice.
Wrath of the Lich King was the very peak of WoW's popularity. They've already given us the nostalgia feels for other eras of WoW. They've yet to go back to Northrend apart from the Death Knight order hall campaign, and that unto itself has some heavy implications.
When I was watching the 3 ads that Blizzard put out for BfA, this is exactly what came to mind. Who are these ads for? Not people who already play. People who used to play. The one with the parents and the baby was particularly on the nose.
Draenor tried the whole 'get players back' thing by being edgy and going back to Burning Crusade nostalgia after people got annoyed by the similarity to Kung Fu Panda (despite Pandaria actually being one of the best WoW expansions to date). But it didn't work. The expansion was terrible. Legion just did its own thing and was great, BFA is just continuing on that path using what worked in Legion (mythic+ and world quests).
Very interesting viewpoint. Thank you for the article.


text:
Q: Solution of differential equation with polynomial coefficients I have come across a rather general form of differential equation and I was wondering if anyone knew what they would be called or where I can find some literature on them.
Imagine we have some function $f$ and we have a list of polynomials $p_0,p_1,...,p_n \in \mathbb{C}[x]$ and $f$ satisfies the differential equation:
$$\sum_{j=0}^n p_j(x) \frac{d^j f}{dx^j} = 0$$
I would like to know results on bounding $f$ and methods of solving for $f$ on the positive real line. Or even just algorithms of solving for $f$. I would also like to know what we would call these differential equations. I thought "homogeneous linear differential equations with polynomial coefficients" might be close but I was wondering if perhaps there was a more exact name. Or if anyone knows of literature that might cover these differential equations, that would be very helpful.
Any help would be greatly appreciated! Thanks!

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Q: Pandas not showing all the columns I asked for, Why? I am new to pandas. The file I am working with is very large and I only want to read a few lines from 9 columns. However, when I run the code, it only shows me 3 of the 9 columns. I don't know why.
columns = [0, 1, 2, 3, 4, 5, 10, 12, 17]
header_names = ['Incident Number', 'Incident Type', 'Report Date', 'Nearest Populated Center',
                'Province', 'Company', 'Substance', 'Significant', 'What Happened category']
p_line = pd.read_csv(r"pipeline_data.csv",
                     skiprows=1,
                     nrows=5,
                     usecols=columns,
                     names=header_names,
                     index_col=0)

This is the ouput...
Also, why is Incident Number not being printed?
                                                    Incident Type  ...    What Happened  category
Incident Number                                                     ...
INC2007-097                                   Release of Substance  ...    Corrosion and Cracking
INC2008-001                                   Release of Substance  ...    Corrosion and Cracking
INC2008-004                                   Release of Substance  ...         Equipment Failure
INC2008-008                                   Release of Substance  ...  Defect and Deterioration
INC2008-009      Release of Substance, Adverse Environmental Ef...  ...         Equipment Failure

[5 rows x 8 columns]

THEN I added
pd.set_option('display.max_columns', None)

but the output was then broken up....
                                                     Incident Type  \
Incident Number
INC2007-097                                   Release of Substance
INC2008-001                                   Release of Substance
INC2008-004                                   Release of Substance
INC2008-008                                   Release of Substance
INC2008-009      Release of Substance, Adverse Environmental Ef...

                Report Date Nearest Populated Center          Province  \
Incident Number
INC2007-097      01/02/2008           Grande Prairie           Alberta
INC2008-001      01/02/2008                   Cromer          Manitoba
INC2008-004      01/23/2008                   Cromer          Manitoba
INC2008-008      01/29/2008              Fort Nelson  British Columbia
INC2008-009      01/29/2008                   Taylor  British Columbia

                                                           Company  \
Incident Number
INC2007-097                                 Alliance Pipeline Ltd.
INC2008-001                                Enbridge Pipelines Inc.
INC2008-004                                Enbridge Pipelines Inc.
INC2008-008      Westcoast Energy Inc., carrying on business as...
INC2008-009      Westcoast Energy Inc., carrying on business as...

                                              Substance Significant  \
Incident Number
INC2007-097                         Natural Gas - Sweet          No
INC2008-001                           Crude Oil - Sweet          No
INC2008-004                           Crude Oil - Sweet          No
INC2008-008                         Natural Gas - Sweet          No
INC2008-009      Potassium Hydroxide (caustic solution)         Yes

                   What Happened category
Incident Number
INC2007-097        Corrosion and Cracking
INC2008-001        Corrosion and Cracking
INC2008-004             Equipment Failure
INC2008-008      Defect and Deterioration
INC2008-009             Equipment Failure

I just want all the info in one long table, not some weird broken up table

A: Maybe you can use this function and look at your table in a new tab
df.to_html('data.html')

A: You can use following config options:

*

*pd.options.display.max_columns = None
It sets the maximum number of columns displayed when a frame is pretty-printed and the truncated lines are replaced by an ellipsis.

*pd.options.display.max_colwidth = None
It sets the maximum width of columns. Cells of this length or longer will be truncated with an ellipsis.

*pd.options.display.width = 1000
You will have to experiment with this value. It sets number of characters displayed.
Other config options are explained here


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Private Equity Executive Search
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Client Manager (Accounts) - Qualified
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Salary £32,000 - £45,000 per annum
Reference IM2701
The perfect candidate for this role would be an ACCA/ACA qualified individual or will definitely consider a strong and capable QBE with a minimum of 3-5 years' experience in a practice environment who is looking to work for a very inclusive employer, who are growing, but have very strong core values and really look after their staff.
Key Roles and Responsibilities:
Accounts preparation for Limited companies, Sole Traders and Partnerships.
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A real tangible opportunity to join a very stable, organically growing practice that really values their staff and has a great culture for you to thrive in the next step of your career.
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The business case for a robust diversity and inclusion strategy in your organisation
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The past few months – and indeed, years – have demonstrated just how important diversity and inclusion are in modern society. Through the #MeToo and Black Lives Matter movements, a light has been shone on the inequality and injustice that persists, not just in our day to day lives, but also in the workplace. We can no longer ignore how important diversity and inclusion are to businesses, nor can we expect things to get better without actively working to improve conditions and outcomes for everyone. And while promoting diversity and inclusion is absolutely the right thing to do for employees, there are also business benefits to doing so. What is diversity and inclusion? Diversity and inclusion aren't just a priority for HR departments – it should be a key business strategy for all organisations. Workplace diversity can be defined as the understanding, acceptance and promotion of differences between people. This includes those of different genders, races, ages, sexual orientations, disabilities and religions, as well as people who have different educational, socioeconomic and experiential backgrounds. Meanwhile, inclusion is about creating a supportive and respectful work environment that values collaboration and participation of all employees, helping everyone to feel included. Together, diversity and inclusion make companies more welcoming, accessible and harmonious for everyone, not to mention more profitable and competitive. Why is diversity and inclusion important? First and foremost, diversity and inclusion are essential to make workplaces better for everyone. Purely from a compassionate perspective, it's the right thing for employers to create environments where people feel comfortable to be themselves and can succeed without limitation. Commercially, diversity and inclusion have a significant number of benefits. Firstly, a strong focus on D&I can significantly widen the candidate talent pool , giving you access to more candidates who may be excluded by non-diverse hiring strategies. With 70% of job seekers looking for a company's commitment to diversity when applying for new roles, it's clear that you may be missing out on top talent if you neglect to address D&I in your organisation. On top of that, diverse organisations have better business results, higher employee satisfaction and are more innovative, according to Business in the Community . McKinsey research shows that executive teams in the top quartile for gender diversity were 25% more likely to have above-average profitability than those companies who perform poorly in terms of executive-level gender diversity. This figure jumps to 36% when analysing teams with ethnic diversity. Diverse teams have also been proven to be more innovative, solve problems faster and have more engaged employees. Small steps to move the dial on D&I in your organisation The current emphasis on working from home presents a key opportunity for employers to rethink their D&I hiring strategies, with current conditions potentially opening up more flexible, part-time opportunities for those who may not have otherwise been able to commit to a 9-5 office job. To welcome more working parents and caregivers, disabled people and those with neurodiversity requirements, consider whether vacancies could be flexible, remote working or on part-time hours. Now is the perfect time to rethink your workspace and how it can be made more accessible to more people. A dedicated diversity and inclusion policy, taskforce or officer can help to highlight its importance within your business. You could perform a D&I audit, examining the levels of diversity that exist within the company and specifically at the executive level, and set goals to achieve a more balanced, inclusive environment within a certain time period. Have open conversations with your team members about D&I and ask them what would make them – and new team members – feel more welcomed. It's also important to acknowledge the diversity that already exists in your company, such as by celebrating different cultural and religious events, greeting bilingual employees in their mother tongue or inviting families into work. Finally, while diversity and inclusion should be championed at the very highest levels of your business, it's crucial that every single team member feels safe to contribute to these discussions and voice their opinions and stories. Prepare to tackle some difficult topics and be questioned. While subjects like the gender pay gap, lack of executive-level diversity and opportunities for progression can feel difficult to address, they are important conversations that need to be had in the process of making real change. Marks Sattin can help to diversify your talent pool. By partnering with a specialist recruitment agency which has a strong focus on diversity and inclusion , you'll benefit from having access to more candidates and guidance on how to actively recruit from diverse talent pools. At Marks Sattin, we can help you identify, attract and retain exceptional people across financial services, technology, change management and more. Contact us here to have a chat about how we can work together.
The past few months – and indeed, years – have demonstrated just how important diversity and inclusion are in modern society.
Becky Hughes
Market Insights 2019 | Commerce and Industry, London
Part Qualified & Transactional The beginning of this year was busier than usual, with consistent job flow and healthy activity - junior professionals are less worried about moving roles with Brexit on the horizon. New budgets are set at the beginning of the year, and companies were certainly hiring. Newly Qualified 2019 got off to a subdued start, it's often a challenging period where clients are busy with month/quarter/year end, and recruitment isn't the priority. In addition to this, the political landscape made employers much more considered about their hiring strategy and we saw 17% less permanent roles being registered. In saying this, as the year has progressed, the volume of roles has increased and the market is buoyant. Our main challenge is that the market is in short supply of candidates. Reasons for this are that Q1 is a busy season for ACAs in practice so they do not have the time to interview, and also many permanent candidates are reluctant to move due to economic uncertainty. Qualified Interim The qualified and interim market enjoyed a successful 2018, with a record Q2 for the business. Albeit a slow start to 2019, the market gained some momentum after January. These peaks and troughs perhaps mirror the political uncertainty around how we are to leave the EU. Despite the turbulent environment, our data points towards a busy recruitment market where we are seeing increases in the number of permanent and interim roles being registered. Considering the UK's high employment rate and the demand for high calibre individuals, there is an emphasis on engaging with passive candidates in new and innovative ways. INSIGHT: Will IR35 affect your business? Employees in the United Kingdom can be categorised as full-time, part-time, casual, freelance and contract workers, with the self-employed bracket now making up 15% of the entire working population. The number of self-employed workers jumped from 3.3 million in 2001 to 4.8 million in 2017, with a corresponding fall in the unemployment rate showing the overall boost in jobs growth from the rise in self-employment. Download the full Commerce & Industry 2019 Market Insight Report » View salaries and commentaries in other UK regions and Ireland »
The beginning of this year was busier than usual, with consistent job flow and healthy activity - junior professionals are less worried about moving roles with Brexit on the horizon. New budgets are set at the beginning of the year, and companies were certainly hiring.
Pres Pillai
Will IR35 affect your business?
Employees in the United Kingdom can be categorised as full-time, part-time, casual, freelance and contract workers, with the self-employed bracket now making up 15% of the entire working population. The number of self-employed workers jumped from 3.3 million in 2001 to 4.8 million in 2017, with a corresponding fall in the unemployment rate showing the overall boost in jobs growth from the rise in self-employment. However, the attractive market for freelancers and contractors has been hit with some uncertainty in recent times, thanks largely to the 2018 Autumn Budget's announcement of IR35 tax reforms. Here's what the new IR35 rules could mean for you and your business: What is IR35? IR35 is a piece of legislation originally introduced to the UK in 1999. Its purpose is to differentiate between those workers who operate as genuine contractors and those who work as 'disguised' employees to avoid paying tax. It came about to challenge contractors who were taking advantage of the tax efficiencies of working through a limited company, with the aim of defending both the Exchequer from lost taxes and protecting workers' rights from unscrupulous employees. However, the IR35 has proven to be ambiguous for many, with some contractors taking advantage of loopholes and a lack of clarity. Hence, the new IR35 rules aim to tighten up the contractor market and ensure tax avoidance loopholes are closed. How does IR35 work? There are three principles that can help to determine employment status and whether a contractor falls inside or outside IR35: Control (the degree of control the client has over the work a contractor does and how and when they do it) Substitution (whether the worker needs to do the work themselves or if they could send a substitute in their place) Mutuality of obligation (whether the employer is obliged to offer work and the contractor is obliged to accept it). Additionally, the contract type, provision of equipment and whether a worker is "part and parcel" of a business can all help to determine whether someone falls inside or outside IR35. The change in IR35 rules shifts the responsibility to determine tax status away from the contractor and onto the business that takes them on. Until now, contractors have been able to self-determine their status, however as of April 2020, when the new rules come into effect for the private sector, companies will risk being fined if they don't make the correct assessment. How will IR35 impact contract workers? It's anticipated that many contract workers who have been enjoying the tax benefits of working outside IR35 will fall under the legislation when employers are tasked with determining their status. This will see more contractors having tax and National Insurance contributions deducted from their pay. However, if you operate as a legitimate small business and are determined to work outside of IR35, you will not be affected by the rule changes. How will IR35 impact employers? The major change for businesses is that they will now be responsible for determining the IR35 status of any contractor working for the company. The new rules will only apply to medium and large sized businesses, so contractors who work for small businesses can continue to set their own IR35 statuses. Those businesses that the IR35 rule changes do apply to will face paying back taxes and fines should they be found to be noncompliant. What should I do to prepare for IR35? Contractors may wish to speak to an accountant or personal finance expert to determine whether IR35 will impact them and if a move to permanent work may prove to be more beneficial after the rules come into effect. For many, contracting will remain appealing regardless of increased tax responsibilities, however it's important to factor in any change in income that IR35 may bring about. Businesses are being warned not to make blanket assessments that cover all their contractors, as this can leave workers without a fair assessment and risk them paying unnecessary taxes without equivalent employment rights. Instead, businesses should consider IR35 status on a case-by-case basis or they may risk losing out on top talent. The HMRC has released a consultation document for businesses to prepare for the IR35 changes, recommending identifying and reviewing current contract workforce status and putting processes in place for taking on new workers. At Marks Sattin, we pride ourselves on keeping abreast of all industry legislation, updates and changes that affect our candidates and clients. Speak with us about how we can help you. References: https://www.bbc.co.uk/news/business-44887623 https://www.ons.gov.uk/employmentandlabourmarket/peopleinwork/employmentandemployeetypes/articles/trendsinselfemploymentintheuk/2018-02-07 https://www.contractorcalculator.co.uk/what_is_ir35.aspx https://www.axa.co.uk/business-insurance/business-guardian-angel/how-ir35-changes-will-affect-freelancers-and-self-employed-contractors/ https://www.telegraph.co.uk/business/ir35-rules/new-contractor-tax/ https://www.telegraph.co.uk/business/ir35-rules/how-will-new-rules-impact-business/ HMRC consultation document
Employees in the United Kingdom can be categorised as full-time, part-time, casual, freelance and contract workers
Options: 109, 129, 4, 48, 79, 89


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The new scientific toolbox is being used to poke around in HDL's house, only to find good news and bad news. HDL has been labeled "good" cholesterol because it helps remove damaged LDL cholesterol from your arteries and has generally been associated with having less cardiovascular disease. It is now coming to light that the quality of the HDL you have is as important, if not more important, than the amount of HDL you have. This means there is both "good HDL" and "bad HDL" and if you have too much of the bad HDL then it no longer protects you and actually helps cause heart disease. How do you know if you have good or bad HDL?
HDL is small in comparison to LDL, and it is higher in protein. It functions as a tow truck, latching on to spent or damaged LDL and returning it to your liver for recycling and/or clearance. The two main proteins that make up HDL are called apoA-I (75%) and apoA-II (25%). ApoA-I is the good guy, and its integrity of structure is vital for HDL's ability to clear damaged LDL from your circulation and the walls of your arteries.
New discoveries are showing that apoA-I is also vital for HDL's enzyme functions that give it anti-inflammatory and antioxidant activity. The role of apoA-II is much less understood, other than to say it is implicated as part of problems with fat metabolism and too much of it causes poor HDL function.
One aspect of HDL fitness is that as it does its work its supply of apoA-I is temporarily diminished and replaced by apoA-II. If HDL then fails to replenish apoA-I it loses its ability to function in a helpful cardiovascular way and actually becomes a problem to cardiovascular health. One key sign that a person lacks apoA-I and has too much apoA-II is elevating triglycerides.
I have long advised that your triglycerides should never be more than twice your HDL, a relationship that in my opinion is far more important than your LDL/HDL ratio. The new science helps clarify why this is the case, explaining that as triglycerides go up then HDL quality goes down. In this handicapped condition HDL loses its ability to remove LDL, quench inflammation, and perform antioxidant functions.
What really has the science world buzzing is a newly recognized function of HDL as a major signaling molecule in your circulation, one that is acting as a communication platform to help instruct other cells around it what to do. Researchers have proven direct communication from HDL to the endothelial cells that line your arteries, the smooth muscle that comprises your arterial walls, the macrophages that are involved with LDL-related plaque formation, and T cells of your immune system.
These new insights came from several lines of research. Advanced proteomic analysis of HDL found that it had approximately 100 small protein peptides that were previously unknown. These small proteins are using the cell membrane of HDL cholesterol as a communication platform. This means that HDL is involved in all sorts of communication that regulates the world around it - a function that has never before been understood and one that is just now being learned about.
Other research has more accurately defined the nature of the fatty substances that make up the HDL cell membrane. These are rich in phospholipids (phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, and phosphatidylinositol). These phospholipids are linked to a unique cell membrane fat called sphingomyelin, which is used to make a major signaling molecule (Sphingosine-1-phosphate).
The discoveries of these multiple research efforts show that HDL is a dynamic communication platform, and has at least 100 different signaling proteins it can use to orchestrate what is going on around it. You might say that HDL cholesterol is a mini-brain floating around in your circulation. It is like having a manager on the production floor.
HDL can become damaged or "spent" at which point it no longer does any of these good things and instead actually contributes to cardiovascular disease, even winding up with LDL in plaque. There are three main reasons this happens.
1) The failure to provide adequate nutrition to re-energize HDL after it has been out working. This leads to a lack of apoA-I and an HDL cell membrane that has lost functionality.
2) Oxidative damage to apoA-I, caused by inflamed and overheated immune cells. This means individuals with inflammatory health issues will have poor quality HDL. The greater the inflammation the worse the HDL quality.
3) Sugar glycation of HDL, rendering it "cemented" so that it can't work. The more uncontrolled the blood sugar, the worse the HDL problem.
This new knowledge gives you three important areas to work on to not only boost your HDL number but to also boost your HDL quality. It is interesting indeed that HDL-building nutrients like niacin and pantethine also help lower triglycerides and improve cardiovascular health, giving more proof to this new field of emerging HDL science.
• Niacin: Niacin has been shown to directly boost your levels of apoA-I while lowering triglycerides. I recommend non-flushing inositol hexanicotinate.
• Pantethine: Pantethine provides the energy to help form HDL, while also providing energy to assist triglyceride and LDL cholesterol metabolism.
• Phosphatidyl Serine: This nutrient contains a mix of the key phospholipids that are often lacking in the diet and needed to construct the healthy cell membrane of HDL.
Along with HDL-building nutrients it is vital to take anti-inflammatory nutrients that calm down immune cells that are inducing free radical damage to the apoA-I protein. The basis of this approach is a diet rich in fruits and vegetables. Many nutrients may be of assistance. Some top choices include grape seed extract, resveratrol, tocotrienols (vitamin E), vitamin C, DHA, pomegranate, and blueberries. Stress management and getting adequate sleep are essential.
Ensure your fasting blood sugar never gets above 90. If it is, use "anti-glycating" nutrients that help protect your HDL from sugar-induced damage. Top choices include R-alpha lipoic acid, grape seed extracts, and resveratrol.
HDL cholesterol is a pivotal molecule that protects your circulation and directly manages its health. It is no longer adequate to simply have an HDL score above 40. You need high quality HDL - HDL that is energized and ready for duty.
Biography: Byron J. Richards, Founder/Director of Wellness Resources, Inc., is a Board-Certified Clinical Nutritionist and a world renowned natural health expert. He is the leading expert on Leptin and weight loss.
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text:
namespace content {

gin::WrapperInfo GCController::kWrapperInfo = {gin::kEmbedderNativeGin};

// static
void GCController::Install(blink::WebFrame* frame) {
  v8::Isolate* isolate = blink::mainThreadIsolate();
  v8::HandleScope handle_scope(isolate);
  v8::Handle<v8::Context> context = frame->mainWorldScriptContext();
  if (context.IsEmpty())
    return;

  v8::Context::Scope context_scope(context);

  gin::Handle<GCController> controller =
      gin::CreateHandle(isolate, new GCController());
  v8::Handle<v8::Object> global = context->Global();
  global->Set(gin::StringToV8(isolate, "GCController"), controller.ToV8());
}

GCController::GCController() {}

GCController::~GCController() {}

gin::ObjectTemplateBuilder GCController::GetObjectTemplateBuilder(
    v8::Isolate* isolate) {
  return gin::Wrappable<GCController>::GetObjectTemplateBuilder(isolate)
      .SetMethod("collect", &GCController::Collect)
      .SetMethod("collectAll", &GCController::CollectAll)
      .SetMethod("minorCollect", &GCController::MinorCollect);
}

void GCController::Collect(const gin::Arguments& args) {
  args.isolate()->RequestGarbageCollectionForTesting(
      v8::Isolate::kFullGarbageCollection);
}

void GCController::CollectAll(const gin::Arguments& args) {
  // In order to collect a DOM wrapper, two GC cycles are needed.
  // In the first GC cycle, a weak callback of the DOM wrapper is called back
  // and the weak callback disposes a persistent handle to the DOM wrapper.
  // In the second GC cycle, the DOM wrapper is reclaimed.
  // Given that two GC cycles are needed to collect one DOM wrapper,
  // more than two GC cycles are needed to collect all DOM wrappers
  // that are chained. Seven GC cycles look enough in most tests.
  for (int i = 0; i < 7; i++) {
    args.isolate()->RequestGarbageCollectionForTesting(
        v8::Isolate::kFullGarbageCollection);
  }
}

void GCController::MinorCollect(const gin::Arguments& args) {
  args.isolate()->RequestGarbageCollectionForTesting(
      v8::Isolate::kMinorGarbageCollection);
}

}  // namespace content


What is the DynOmics ENM server?
The DynOmics ENM server computes biomolecular systems dynamics for user-uploaded structural coordinates or PDB identifiers, by integrating two widely used elastic network models (ENMs) – the Gaussian Network Model (GNM) and the Anisotropic Network Model (ANM). Unique features include the consideration of environment, the prediction of potential functional sites and reconstruction of all-atom conformers from deformed coarse-grained structures. For more information see Theory and Tutorial.
ENM result will be responsed in a few seconds for input structure with <2,000 residues.
A result page link here can be bookmarked for submissions with a big structure.