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Streptomyces thermoautotrophicus does not fix nitrogen
Drew MacKellar1,2,
Lucas Lieber3,4,
Jeffrey S. Norman5,
Anthony Bolger6,
Cory Tobin7,
James W. Murray4,
Mehtap Oksaksin6,
Roger L. Chang1,
Tyler J. Ford1,
Peter Q. Nguyen2,
Jimmy Woodward5,
Hugo R. Permingeat3,
Neel S. Joshi2,
Pamela A. Silver1,2,
Björn Usadel6,
Alfred W. Rutherford4,
Maren L. Friesen5 &
Jürgen Prell6
Scientific Reports volume 6, Article number: 20086 (2016) Cite this article
Streptomyces thermoautotrophicus UBT1 has been described as a moderately thermophilic chemolithoautotroph with a novel nitrogenase enzyme that is oxygen-insensitive. We have cultured the UBT1 strain and have isolated two new strains (H1 and P1-2) of very similar phenotypic and genetic characters. These strains show minimal growth on ammonium-free media and fail to incorporate isotopically labeled N2 gas into biomass in multiple independent assays. The sdn genes previously published as the putative nitrogenase of S. thermoautotrophicus have little similarity to anything found in draft genome sequences, published here, for strains H1 and UBT1, but share >99% nucleotide identity with genes from Hydrogenibacillus schlegelii, a draft genome for which is also presented here. H. schlegelii similarly lacks nitrogenase genes and is a non-diazotroph. We propose reclassification of the species containing strains UBT1, H1 and P1-2 as a non-Streptomycete, non-diazotrophic, facultative chemolithoautotroph and conclude that the existence of the previously proposed oxygen-tolerant nitrogenase is extremely unlikely.
Biological fixation of gaseous elemental nitrogen into ammonia is an important part of the global nitrogen cycle1. Several bacteria and archaea are known to carry out this process, either solely for their own biosynthetic needs2, or else as symbiotic partners with certain eukaryotes3. In many environments, bioavailable nitrogen is limiting for net primary productivity4 and this relevance for agriculture and ecology has ensured that the biology of nitrogen fixation has been an active area of research since its discovery in the 19th century.
Bacteria synthesize ammonia from dinitrogen via a family of homologous nitrogenase enzymes, of which the molybdenum-iron (MoFe) nitrogenase is the most common5. The MoFe nitrogenase protein combines with the homodimeric nitrogenase reductase Fe-protein to form an (αβγ2)2 octameric enzyme complex6, encoded by the structural genes nifHDK. Each enzyme complex contains two iron-sulfur (4Fe:4S) clusters, two (8Fe:7S) P-clusters and two (7Fe:Mo:9S:C:homocitrate) FeMo cofactor active sites. Several accessory proteins are required for the synthesis and insertion of these complex cofactors and thus various subsets of some 16 different additional nif genes are present in diazotrophs7. A set of six nif genes, nifHDKENB, has been proposed as a diagnostic criterion for diazotrophy8. The MoFe nitrogenase has promiscuous activity and is able to reduce a number of substrates besides dinitrogen9. Of particular interest is its ability to convert acetylene into ethylene, which is often used as a proxy assay for nitrogenase activity.
While the exact mechanism of nitrogenase function remains unknown10, the stoichiometry is thought to require at least 16 molecules of ATP, as well as six low-potential electrons from ferredoxin or flavodoxin, per molecule of dinitrogen reduced11, plus two more electrons to generate one molecule of hydrogen, an obligatory side product of nitrogenase function. In addition to this high energetic cost, nitrogenase activity is reversibly inhibited by hydrogen (H2) and carbon monoxide (CO)12; the former of which is generated by nitrogenase itself and the latter generated by heme oxygenase in root nodules13, as well as being present in significant concentrations near sites of geothermal activity or combustion. More importantly, nitrogenase is rapidly and irreversibly damaged upon exposure to oxygen14, due to the effect of oxygen on the enzyme's metal cofactors15, perhaps exposed to the solvent upon the cyclic dissociation of the nitrogenase and the nitrogenase reductase16. This sensitivity has led to a proliferation of evolutionary strategies to reduce oxygen tension near active nitrogenases17.
In addition to the MoFe nitrogenase, some bacteria possess alternative nitrogenases that substitute iron or vanadium for molybdenum in the active site18, presumably for use when Mo is scarce. These alternative nitrogenases introduce a δ subunit encoded by vnfG or anfG, altering the stoichiometry of the nitrogenase complex, but the structural vnfHDK and anfHDK genes are homologous to their nif counterparts and the corresponding enzymes have even greater sensitivity to O2, as well as lower nitrogenase activity and greater relative hydrogen production, than the MoFe enzyme.
A fourth, unrelated class of nitrogenase has been reported in the thermophilic carboxydotroph Streptomyces thermoautotrophicus UBT119. This strain was isolated from the soil overlying burning charcoal piles and was studied by members of the laboratory of Ortwin Meyer, at the Universität Bayreuth, Bayreuth, Germany. UBT1 was described as an obligate chemolithoautotroph, capable of growth on CO or CO2 and H2, but not on complex carbon sources20. Nitrogen fixation in this strain was found to be significantly different from that of other diazotrophs: it was not inhibited by H2, CO, or O2. Further, nif genes were not detected by Southern blot and the strain was not found to reduce acetylene to ethylene, suggesting that this was the first natural diazotroph to lack a nitrogenase homologous to MoFe. Fractionation of protein lysate was used to identify structural components of the nitrogenase and the N-termini of the proteins isolated showed no homology to known nitrogenase subunits21. The specific activities reported for this enzyme were unusually low in comparison to traditional nitrogenases. Most interesting of all, the enzyme was found to be insensitive to oxygen in cell lysates and actually depended upon its presence for the generation of the superoxide anion (O2−), which was proposed to be used as an electron donor for the reduction of dinitrogen.
A catalytic mechanism was proposed whereby CODH (enzyme St3) transfers electrons from water to oxygen via the oxidation of CO, a manganese superoxide dismutase (Mn-SOD; enzyme St2) transfers electrons from the resulting superoxide anion to a molybdenum hydroxylase, likely another CODH homolog (St1), which in turn uses them to reduce dinitrogen. The CODH that generates superoxide was found to have no nitrogenase activity21. Degenerate oligonucleotides designed against the N-termini of the purified proteins have been used to identify the candidate nitrogenase genes and they were found to be homologous to known carbon monoxide dehydrogenases (CODH; enzyme St1 encoded by sdnMSL) and a superoxide dismutase (enzyme St2 encoded by sdnO)22.
Stimulated by these early results, we have sequenced the genome of S. thermoautotrophicus UBT1, provided by the original isolator, D. Gadkari, in two independent laboratories. We have also isolated two novel strains of S. thermoautotrophicus with the described phenotypic characters: strain H1 from another burning charcoal pile and strain P1-2 from soil near an active coal seam fire. We have sequenced the H1 strain and find an average of 95% identity between its coding sequences and those of UBT1. The genome of strain P1-2 was not sequenced. Both draft genome sequences for strain UBT1, as well as the draft genome for strain H1, lack known nitrogenase genes (nif, vnf, or anf). Further, all three draft genomes lack coding sequences with high identity to the published sdn genes; instead we note that the sdn genes previously published show high identity with genes in Hydrogenibacillus schlegelii DSM2000, a draft genome of which is also presented here. Finally, we find that the H1 and UBT1 genomes possess multiple loci encoding CODH enzymes, which is also the case in another organism previously reported to be a diazotroph and whose published genome sequence similarly lacks nif genes: Pseudonocardia dioxanivorans. We accordingly included this strain in our characterization of putative novel nitrogen fixation activities.
The UBT1, H1 and P1-2 strains all grow on CO and H2 gas on mineral media, as well as on pyruvate, despite the previous description of UBT1 as an obligate chemolithoautotroph. Growth was not observed on other carbon sources tested. The H1, UBT1, P1-2 and P. dioxanivorans CB1190 strains sustain minimal growth on media lacking NH4, but do not incorporate heavy-isotope-labeled N2 gas (15N2) into biomass as measured in attempts at different sites. These results are inconsistent with nitrogen fixation, but instead suggest that this organism can incorporate ammonia and other combined nitrogen species at exceedingly low concentrations. We propose reclassifying Streptomyces thermoautotrophicus as a non-Streptomycete, non-diazotrophic, facultative chemolithautotroph.
Strain UBT1 and two new strains grow largely as previously described
The sources of the Streptomyces thermoautotrophicus strains used in this paper are summarized in Table 1. We found the morphology and general growth characteristics of all strains to be consistent with the previous description for strain UBT120 (Fig. 1). Specifically, Streptomyces thermoautotrophicus grows rapidly on hydrogen, reaching its maximal extent of growth within three days of incubation at 60 °C on solid media in the presence of a 4:4:1:1 mix of N2:H2:CO2:O2. The strains grow more slowly on a 9:8:2:1 mix of CO:N2:O2:CO2, reaching maximal growth within four days of incubation at 60 °C. Limited germination could be observed on some carbon sources in the absence of H2 or CO, but the only carbon source found to sustain growth reproducibly was pyruvate. Growth in liquid was poor, either in suspension in pyruvate or as a pellicle in the presence of H2 or CO. Serial passage on mineral media solidified with gellan gum and in the presence of H2 or CO was robust, as was serial passage on plates containing pyruvate and NH4 (DSM260 media), suggesting that the initial assessment of this species as an obligate chemolithoautotroph was premature.
Table 1 Sources of S. thermoautotrophicus strains used in this paper.
Morphology of S. thermoautotrophicus.
On mineral media solidified with gellan gum, growth is more robust in the presence (A) than the absence (B) of 30 mM NH4Cl (scale bars are 200 μm in each image). Electron microscopy shows smooth, branching substrate mycelia and hairy/rugose decorations on mature spores, which are arranged in straight, unbranched chains ((C), magnification is 6,810x). Spore formation is robust on media containing NH4Cl ((D), magnification is 3,600x), but when grown on media lacking NH4Cl the ratio of substrate to aerial mycelia is higher and the hyphae assume varied morphologies ((E), magnification is 3,290x). In cross section, unknown vacuoles and other subcellular features appear and may be more prominent in mature spores (lower center, identifiable by rugose exterior) than in other hyphae ((F), magnification is 20,000x).
The strains did not grow above 80 °C or below 42 °C. H1 and UBT1 form a non-fragmenting, branched substrate mycelium and, after at least 24 hours of growth, produce aerial hyphae that septate into chains of grey-pigmented spores, the most mature form of which bear hairy to rugose surface decorations23. Substrate hyphae, aerial hyphae and free spores are all highly hydrophobic and remain buoyant atop aqueous solutions.
S. thermoautotrophicus strain H1 was isolated from soil obtained from a charcoal pile in Hasselfelde, Germany, by adding soil particles to liquid media under a hydrogen atmosphere and transferring the resulting buoyant mycelia after 9 days of culture. The original liquid phase enrichment of S. thermoautotrophicus from soil retained spores that formed non-mycelial colonies, which grew well on complex media. 16S rRNA analysis of these colonies indicated that they were primarily related to thermophilic Firmicutes, including Bacillus, Brevibacillus and Geobacillus. Interestingly, liquid phase growth of this initial consortium resulted in extensive pellicle growth, which was lost upon subsequent purification of S. thermoautotrophicus after serial passage and selection of single colonies on plates. Growth of the selected strain on plates remained robust, however.
S. thermoautotrophicus strain P1-2 was isolated from soil situated near an active coal seam fire in Centralia, PA, USA. Its nutritional requirements were identical to strain H1. PCR with universal RNA primers (27F and 1492R24) produced a single amplicon, Sanger sequencing of the amplicon indicated 99.3% identity with one of the 16S rRNA loci present in both genomes from the H1 and UBT1 strains (Supplementary Fig. 1).
Genome sequences indicate a novel genus
Streptomyces thermoautotrophicus strain UBT1 was grown and sequenced independently at two different facilities (Rosario, Argentina and Aachen, Germany) and S. thermoautotrophicus strain H1 at a third (Boston, MA, USA; Fig. 2). Optical mapping data (OpGen, Gaithersburg, MD) were collected for strain H1 and indicated the presence of a single, circular chromosome of 5.25 Mbp. A combination of short reads (Illumina), scaffolding reads (Pacbio) and optical mapping consolidated genome assembly of strain H1 into a single scaffold 4.90 Mbp in length (of which 4.5 Mbp is sequenced data and 0.4 Mbp is scaffolding Ns), containing an estimated 85% of the genome, with 5 additional contigs >1 kbp in length that could not be aligned to the map (and that could collectively account for another 1.3% of the genome) and one 48 kbp contig that is likely a plasmid, based on the presence of multiple genes encoding phage- and plasmid-related putative functions. The genome sequences of strain UBT1 are more fragmentary, but have similar lengths and gene content, including the possession of a 55 kbp plasmid (which shares no significant similarity with that of the putative H1 plasmid). The genome characteristics are compared to those of other Actinomycetes in Supplementary Table 1. Comparison of the H1 and UBT1 (Aachen) genomes by the Genome Blast Distance Phylogeny (GBDP) approach, as implemented with default settings and bootstrapping on a web server (http://ggdc.dsmz.de; date of access 21/06/2015)25 yielded a distance estimate of 0.0067, comparable to a DNA-DNA hybridization (DDH) value of 94.9%, predicting a 97.18% probability that DDH >70% and thus that the strains belong to the same species.
Genome map of S. thermoautotrophicus H1.
20 scaffolds from the SPAdes assembly were aligned to the optical map and gaps in the chromosome were filled in with N's manually. Six nodes did not align; the largest of which is a predicted plasmid. The two outer rings represent genes on the two strands of the chromosome. The inner ring shows the GC content and prominent dips represent gaps in the assembled sequence; with length and position inferred from the optical map.
The circularity and relatively modest size of the H1 genome are uncommon among Streptomycetes26 and accordingly we undertook analysis of the nearest relatives of these strains. The UBT1 (Aachen) genome assembly contains three small subunit ribosomal RNA genes, while the more fragmentary Rosario genome assembly contains two. The H1 strain genome assembly similarly has two. Each genome contains two different 16S gene sequences that share only 90% identity between them. Between the H1 and UBT1 strains, however, each 16S sequence in one strain matches the corresponding sequence from the other strain with 99% identity. The presence of significantly different ribosomal RNA operons within a single bacterial genome has been observed before, especially in thermophiles27. A phylogenetic tree comparing the 16S sequences shows that one of the 16S genes clusters most closely with Thermobispora bispora and Acidothermus cellulolyticus (Supplementary Figure 2a). The identities with either species are 90%. The other 16S sequence in either genome has various nearest neighbors among other actinomycetes, but shares less than 93% identity over 93% of its length with any of them, as determined by BLAST.
In the absence of conclusive evidence as to which of these divergent rRNA sequences is ancestral to the organism and which may be the product of a more recent horizontal gene transfer or duplication event, we adopted the approach of concatenating 14 well-conserved proteins (13 ribosomal proteins and one phosphpatidate cytidylyltransferase) within the genome sequences of strains H1 and UBT1 and performing alignment and neighbor-joining analyses with similar sequences from all Actinobacteria with fully sequenced genomes that were available at the time of analysis28. The resulting tree, while possessing some nodes with low confidence based on the supporting bootstrap values, is largely in agreement with recent results that have followed the same approach29 (Supplementary Figure 2b). From this approach, it appears that H1 and UBT1 are most closely related to the clade that includes the genera Acidothermus, Streptosporangium, Thermobifida, Thermobispora and Thermomonospora. By this analysis, these strains do not belong in the genus Streptomyces and instead are in the class Actinobacteria, subclass Actinobacteridae, order Actinomycetales. The suborder, family and genus are all undetermined, although nearby families include Frankineae (Acidothermus), Streptosporangineae (Thermomonospora, Streptosporangium, Nocardiopsis, Thermobifida) and Pseudonocardineae (Thermobispora).
Genomes lack nitrogenases; possess multiple CODHs
No nif genes are present in any of the three draft genomes, other than a single gene annotated as "Nitrogen-fixing NifU, C-terminal:Rieske [2Fe-2S] region". This single gene is located between a predicted uptake hydrogenase and factors for the maturation of that enzyme, which is homologous to similar clusters in other organisms. Proteins with C-terminal homology to NifU have predicted functions in metal cofactor synthesis, including in non-diazotrophs and are not diagnostic for the presence of a functional nitrogenase30. The genome also contains no sequences with significant homology to anf or vnf genes for the alternative Fe- or V-nitrogenases.
The superoxide-dependent nitrogenase was previously identified to involve two molybdenum hydroxylases, St1 and St3, which were homologs of CODH. St1 was reported to be the putative dinitrogen reductase and St3 a true CO dehydrogenase responsible for generation of the superoxide anion radical. Both were reported to possess the same heterotrimeric structure of typical CODH enzymes or other molybdenum hydroxylases22. The UBT1 genome contains four operons encoding predicted aerobic CODH and the H1 genome contains three (Table 2). Two of the UBT1 operons contain large subunit (coxL) genes encoding the AYXCSFR motif characteristic of form I aerobic CODH31 and are located near coxDEF or coxG accessory genes necessary for the maturation of the functional CODH complex. The other two loci contain large subunits with alternative motifs that suggest categorization with form II CODH, or other molybdenum hydroxylases32. The medium subunits of all four loci have sequences consistent with the ability to bind flavin adenine dinucleotide (FAD) cofactor, despite the previous finding that St1 (encoded by sdnM) does not contain FAD22. One of the coxMSL loci, present in both the H1 and UBT1 genomes, shows high identity between the encoded proteins and sequences published by Ortwin Meyer in 199331 (Fig. 3), suggesting that the UBT1 strain sequenced here is the same strain as reported on in that period.
Table 2 Predicted carbon monoxide dehydrogenase genes in strains H1 and UBT1 and H. schlegelii, compared to sdn genes.
Gene sequences from the H1 and UBT1 genomes match closely those reported early in the cultivation of UBT1 by the Meyer group.
Protein alignments from S. thermoautotrophicus CODH published by the Meyer group in 1993 versus corresponding sequences from the genomes of UBT1 and H1; CoxM (A), CoxS (B) and CoxL (C), respectively. For brevity, only the first 50 residues are shown for each alignment. N-terminal Edman degradation sequences for CoxM and CoxL are from31. The complete CoxS sequence was obtained from an unpublished dissertation from the Meyer laboratory; the full-length protein sequence possesses 100 percent identity with either H1 or UBT1 CoxS. Residues that are identical are black; residues that are similar to the consensus are colored green; all others are colored red.
The identity between the N-terminal protein sequences published for the St1 proteins with any of the UBT1 or H1 CODH sequences, however, is low (maximum identity between any H1 or UBT1 CODH protein and St1M, S and L respectively is 53%, 50% and 35%)21. Nucleotide sequences coding for the St1 subunits, based on sequencing clones from a library of DNA that hybridized with degenerate oligonucleotides derived from the N-terminal St1 sequences, have previously been determined33. These sequences have also recently been uploaded to the GenBank database (GI: 589823865, 589823867 and 589823869, for sdnMSL, respectively). The highest identity of any of the proteins encoded by these genes to any of the H1 or UBT1 sequences at the amino acid level is 50%, 63% and 62%, for the proteins encoded by sdnM, S and L, respectively (Table 2). These proteins in our S. thermoautotrophicus genomes are all annotated as CoxM, S and L proteins.
The St2 protein was previously identified as a manganese-dependent superoxide dismutase (Mn-SOD), encoded by the sdnO gene. Similarly to St1, a nucleotide sequence for the entire sdnO gene had been recorded33 and has recently been uploaded to GenBank (GI:588295007). The UBT1 and H1 genomes each contain one predicted Fe/Mn-SOD; identity with the St2 protein sequence encoded by the reported sdnO gene sequence is 38% at the amino acid level (Table 2).
Superoxide-Dependent Nitrogenase genes match Hydrogenibacillus schlegelii
The proteins encoded by sdnMSL and sdnO have higher identities, however, with proteins from other strains which have partial sequences within the GenBank database. A BLAST search revealed that the closest match for sdnL was to a partial coxL gene sequence (GI:38679249) in Hydrogenibacillus schlegelii (formerly Bacillus schlegelii), for which no full genome sequence was available. In order to investigate the relationship between the sdn sequences and H. schlegelii, we undertook sequencing the genome of that organism as well and report here a draft sequence for the genome of H. schlegelii strain DSM2000. This strain is a known chemolithoautotroph, with similar optimum temperature for growth as S. thermoautotrophicus and similar capacity to grow on CO or H2. Accordingly, the draft genome contains genes encoding ribulose bisphosphate carboxylase/oxygenase (RuBisCo), phosphoribulokinase (PRK), CODH and uptake hydrogenase. H. schlegelii has not been described as a diazotroph. Its genome lacks nif/anf/vnf nitrogenase genes and we found that the strain failed to grow on media lacking NH4Cl, with no visible turbidity in cultures incubated for up to one month, either when grown chemolithotrophically on H2/CO2, or when grown heterotrophically on pyruvate.
The DSM2000 genome contains a single locus encoding a putative molybdenum CODH operon (TR75_12445-55) with >99% identity with the sdn operon at the nucleotide level. Translations of the open reading frames (ORFs) within also demonstrated >99% identity to the St proteins (Table 2). It was further determined that a Mn-SOD within the H. schlegelii genome (TR75_10445), bore 100% identity at the amino acid level to the St2 protein (Table 2). Extensive overlap was also found at the nucleotide level between the DSM2000 genome sequence and the region around the coding sequence for sdnO (99.62% identity, over 7.2 kbp), previously sequenced by the Meyer group.
Strains grow on media lacking NH4Cl, but fail to incorporate significant 15N2 into biomass
All S. thermoautotrophicus strains tested were capable of continuous culture on mineral media lacking added NH4+ but peculiarities regarding their growth were apparent. Specifically, growth was far more robust in the presence of 30 mM NH4+. Furthermore, growth was only supported on plates solidified with 0.5–1.0% gellan gum, or on agar fortified with charcoal or bio-char. A CHN analysis on the gellan gum used returned a nitrogen content of 0.095%. Growth on mineral media solidified with Noble agar was not observed. In addition, growth on plates containing either gellan gum or agar plus bio-char was greatly enhanced by the presence of plates containing 30 mM NH4+ within the same atmosphere, presumably due to the former cross-feeding on NH3 volatilizing from the latter.
The high number of CODH enzymes encoded by the S. thermoautotrophicus genomes and the purported identity of the St1 nitrogenase as a modified CODH prompted us to examine other published genomes containing high numbers of such enzymes. We identified Pseudonocardia dioxanivorans CB1190 as another strain whose sequenced genome encodes multiple molybdenum hydroxylase enzymes (10 operons, two of which are putative type I CODH enzymes34) and which has been reported to be a diazotroph35. The strain CB1190 similarly lacks nif, anf and vnf genes, yet reportedly displayed a similar ability to grow on media lacking NH4+ as an additive. This strain was accordingly included in subsequent experiments to test for diazotrophy using isotope labeling.
In order to determine whether the growth of our strains was independent of exogenous combined nitrogen, we undertook labeled nitrogen assays at four independent laboratories (Table 3). Plates were inoculated with spores of the strains indicated and incubated in the presence of acid-washed 15N2 gas. After 3–5 days, biomass was scraped from plates and subjected to isotope-ratio mass spectrometry (IRMS). At three sites with thorough 15N2 gas washing, no enrichment of 15N was detected in biomass relative to plates supplemented with NH4Cl. At one site, a slight enrichment of 15N in biomass was observed. We note, however, that the 15N2 gas used in this experiment (Aldrich catalog #364584, Lot#SZ1670V) was found in a recent report36 to contain significant amounts of contaminating 15NH4+ and 15NO3−/NO2−. The small amount of 15N detected in the biomass of our samples, is much less than the amount that would be present in unwashed gas (Supplementary Fig. 3); thus it is possible that not all of this contaminating fixed 15N was captured by washing for 30 minutes in 5% H2SO4. Fixation was also not detected in P. dioxanivorans CB1190.
Table 3 Results of growth of selected strains in the presence of 15N2 gas.
We conclude that there is no evidence for and substantial evidence against, the existence of an oxygen-tolerant nitrogease as previously described in Streptomyces thermoautotrophicus. Our major empirical lines of evidence are both genomic and phenotypic. First, using genome sequencing, we failed to find either canonical or the proposed novel nitrogenase enzymes in our S. thermoautotrophicus isolates. Rather, sequences of the proposed enzymes are present in another thermophilic, non-diazotrophic bacterium. Second, extensive multi-site fixation experiments using 15N2 gas failed to demonstrate incorporation into biomass. Growth experiments suggest that S. thermoautotrophicus strains are highly effective nitrogen scavengers, but not nitrogen fixers.
Finally, we point out some other inconsistencies in the original characterization of the proposed oxygen-tolerant nitrogenase system. Apart from the superoxide dependent nitrogenase, no known aerobic reduction of nitrogen to ammonia is known. An unprecedented mechanism in the scheme is the use of superoxide as an electron donor for a biologically productive reaction. No other productive biological use of superoxide is known, although many cells produce superoxide as a toxin. Usually superoxide is a toxic byproduct of cellular metabolism. With a standard potential of −330 mV37 it is comparable to the NADH or NADPH potential of −320 mV, but much more toxic due to its reactivity. The Fe protein of the MoFe nitrogenase has an apparent midpoint potential of −470 mV38 and the physiological electron donor is ferredoxin or flavodoxin. Therefore it seems unlikely that superoxide is sufficiently reducing to drive the nitrogenase reaction. In addition, none of the putative superoxide dependent nitrogenase system proteins have a plausible ATPase domain identified, even though ATP hydrolysis was required for the activity. In the original work, labeled nitrogen was not used during the purification of the nitrogenase, instead an ammonia production assay21 was used, based on the reaction of ammonia with sodium phenate and hypochlorite39. This assay is very sensitive, but is known to have a high background40, which may have contributed to a misidentification of nitrogenase activity.
There is absolutely no genomic evidence that S. thermoautotrophicus is diazotrophic. No MoFe, VFe, or Fe-only nitrogenase genes are present in the draft genome sequences presented here. The UBT1 strain was previously found to lack nif genes by dot blotting19 and its putative oxygen-tolerant nitrogenase was characterized by protein lysate fractionation and assays for ammonia production in vitro21. The identities of the three protein complexes of the proposed oxygen-tolerant nitrogenase were previously found to be two variants of molybdenum hydroxylases related to CODH and an SOD-like protein21,22. However, N-terminal sequences of the proposed heterotrimeric St1 nitrogenase and of the homodimeric St2 nitrogenase reductase, as well as the sequences of their respective genes sdnMSL and sdnO, do not match any sequences present in the draft genomes of strain UBT1 or the novel isolate H121,22.
The genes for the proposed oxygen-tolerant nitrogenase are in fact present in the genome of another thermophilic non-diazotrophic bacterium. The sequences for sdnMSL and sdnO match with identities between 99.1% and 100% at the nucleotide level the coxMSL and sod gene sequences from Hydrogenibacillus schlegelii DSM2000, an isolate closely related to H. schlegelii DSM9132, which was studied by the Meyer group in the same period as S. thermoautotrophicus41,42,43. S. thermoautotrophicus and H. schlegelii overlap closely in their nutritional requirements and optimal growth temperature and contamination of cultures of S. thermoautotrophicus is therefore a possibility. If the Meyer group did contaminate their liquid culture of S. thermoautotrophicus with H. schlegelii, these are the sequence data we would observe. We conclude that the St1 and St2 proteins are in fact the aerobic CODH and superoxide dismutase proteins from a strain of Hydrogenibacillus schlegelii.
In addition to the absence of the putative oxygen-tolerant nitrogenase genes, our extensive phenotypic characterization does not support the claim the S. thermoautotrophicus is diazotrophic. The strain of S. thermoautotrophicus we have received from D. Gadkari represent the closest known link to that which was claimed to be diazotrophic in 1992. Independent samples of that strain were cultured by different personnel at two independent sites, found to grow poorly on media lacking NH4Cl, grew reproducibly only on media containing additives that may contribute combined nitrogen and did not incorporate 15N2 into biomass. We fail to see convincing evidence for diazotrophy in this strain. In addition, strain H1, isolated from a burning charcoal pile using procedures detailed for the isolation of UBT1 by the original authors20 and strain P1-2, isolated from soil overlying a coal seam fire, share morphology and physiology with UBT1 that are indistinguishable by any test performed thus far. They qualify as members of the same species as UBT1, according to whole-genome prediction of DNA-DNA hybridization and 16S rRNA sequence identity for strains H1 and P1-2, respectively. While there is no guarantee that independent isolates of a particular species of bacteria will share identical metabolic capabilities, it is striking that the H1 and P1-2 strains, which were not cultivated for prolonged periods in the laboratory before being subjected to 15N2 incorporation assays, show a similar capacity for minimal but reproducible growth on media lacking added NH4Cl, yet also fail to show significant incorporation of acid-washed, labeled dinitrogen into biomass.
Similar to our results in S. thermoautotrophicus, the abundance of CODH paralogs in the genome of P. dioxanivorans failed to predict functional diazotrophy in that strain, as measured by 15N2 assays. Subsequent dialogue with the authors responsible for the isolation and initial characterization of the P. dioxanivorans species confirmed that, contrary to early reports, the sequenced strain lacks known nitrogenase genes and that the strain now available is not diazotrophic (L. Alvarez-Cohen, personal communication).
Other researchers have identified bacteria capable of growth on trace environmental sources of combined nitrogen44,45 and previous claims of diazotrophy in various strains have subsequently been found to be premature46,47. A high-affinity pathway for assimilation of scarce combined nitrogen may prove a viable alternative to the maintenance of a complex pathway for nitrogen fixation when ammonia and nitrate are scarce. Efficient scavenging of combined nitrogen is certainly a more parsimonious explanation for our data than a novel nitrogenase that is orthogonal to all known systems and insensitive to oxygen.
While it remains possible that novel nitrogenases will be uncovered as the resources for querying the genomes of unculturable bacteria advance, any such enzymes must be supported by sensitive functional assays in order to verify their activities. Among such assays, measuring the incorporation of isotopically-labeled dinitrogen gas into biomass by mass spectrometry remains the most sensitive and specific48,49. The high sensitivity of isotope-ratio mass spectrometry, however, can also be a liability, as when the input reagent may be contaminated with fixed 15N, a fact that may have contributed to the confusion regarding the status of UBT1 and other strains as putative diazotrophs.
We cannot rule out the possibility that the strains used in the previous publications cited possessed a novel nitrogenase, which was subsequently lost upon prolonged cultivation in the laboratory. Any such enzyme, however, would likely not be constituted of subunits with the sequences previously published, as their presence in H. schlegelii does not correspond with the ability to fix nitrogen, nor does their absence in UBT1 limit its ability to grow on media containing no added NH4Cl. Should a viable sample of diazotrophic UBT1 be recovered, we welcome the chance to characterize it further. However, given the evidence in hand we conclude that the existence of the proposed oxygen-tolerant nitrogenase system is extremely unlikely.
Regarding the reassignment of the phylogeny of this species: the identity between the 16S rRNA sequences in the H1, UBT1 and P1-2 strains is sufficiently high to warrant their classification as members of a single species. When compared to other Actinobacteria, however, identity falls below the commonly suggested guidelines for placing these strains within a known genus50. More sensitive protein sequence alignments support this divergence and suggest an early-branching member of the order Streptosporangiales. It is unclear at the current level of resolution available from full genome sequences whether these strains best fit as members of the family Nocardiopsaceae, Thermomonosporaceae, or a new family. In any case, the level of divergence from categorized species is sufficient to recommend that Streptomyces thermoautotrophicus be reassigned to a new genus and described as a non-diazotroph on the basis of this work.
The UBT1 (Rosario), H1 and P1-2 strains have been deposited in the Leibniz-Institut DSMZ (Deusche Sammlung von Mikroorganismen und Zellkulturen GmbH) culture collection, with catalogue numbers DSM 100163, DSM 100164, DSM 100422 respectively.
Strain isolation and cultivation
S. thermoautotrophicus strain UBT1 was kindly provided by the original author Dilip Gadkari, Bayreuth, Germany to research groups at the Universidad Nacional de Rosario, Argentina and the RWTH Aachen, Germany. At the Universidad Nacional de Rosario, S. thermoautotrophicus strain UBT1 was grown in DSMZ Medium 811, in a defined atmosphere of 60% CO, 20% CO2, 20% air. Plates were grown on 1.5% agar supplemented with 0.5% activated charcoal. At the RWTH Aachen, S. thermoautotrophicus strain UBT1 was grown in NFIX mineral media supplemented with Drews elements (in μg/L: Na-Fe-EDTA 800, MnCl2 10, CoCl2 2, CuSO4 1, ZnCl2 2, LiCl 0.5, SnCl2*2H2O 0.5, H3BO3 1, KBr 2, KI 2, BaCl2 0.5, pH 6.0) in a defined atmosphere of 45% CO, 5% CO2 and 50% air.
S. thermoautotrophicus strain H1 was isolated from soil taken from a charcoal pile in Hasselfelde, Germany. Soil particles were immersed in mineral media20 and incubated in the presence of H2 and CO2 at 60° C. Pellicles emerged after several days and were transferred and enriched on plates of mineral media plus 0.5% gellan gum (Sigma) and 0.075% MgCl2*6H2O in a defined atmosphere of 40% H2, 10% CO2 and 50% air, or else in 45% CO, 5% CO2 and 50% air51.
S. thermoautotrophicus strain P1-2 was isolated from soil located above an underground fire where a coal seam has been burning for several decades (Centralia, PA)52. The soil temperature in situ at the time of collection was 60 °C and the collection site was immediately adjacent to steam issuing from a fissure. Isolation proceeded as for the H1 strain, except that isolation was conducted at an independent facility, liquid enrichment was in DSMZ261 mineral media53 and plates were solidified with 1% gellan gum and 0.15% MgCl2.
Pseudonocardia dioxanivorans CB1190 was grown on ATCC medium 19635 for routine maintenance and was grown on NFIX mineral media plus glucose and gellan gum for labeled nitrogen assays. Azotobacter vinelandii strains DJ and DJ100 (ΔnifD)54 were grown on Burk's medium55 with or without 30 mM NH4Cl for maintenance and labeled nitrogen assays. P. dioxanivorans and A. vinelandii were grown in air at 30 °C. Hydrogenibacillus schlegelii DSM2000 was grown on DSMZ Medium 260 or 26156, for heterotrophic or chemolithotrophic growth, respectively. To test for diazotrophy in this strain, variants of these media to omit NH4Cl were also prepared. H. schlegelii was grown at 55 °C in either air or an atmosphere of 45% H2, 10% CO2 and 45% air.
Labeled nitrogen assay
In all experiments, separate canisters of 15N2 gas were purchased from Aldrich chemical (St. Louis, MO). The gas was acid-washed prior to introduction to the culture vessels: 15N2 gas was retrieved from the canister and injected into a Schott bottle filled with water and sealed with a rubber septum. Water was withdrawn upon introduction of the gas to equalize pressure and allow continued introduction of the gas until approximately half the volume of the bottle was occupied by the gas. Then concentrated H2SO4 was introduced to bring the concentration of H2SO4 in the water within the bottle to 5%. The gas was incubated for the indicated period of time with agitation before being extracted and introduced to the culture chamber.
At Harvard Medical School: 15N2 gas was acid-washed for 30 minutes. Plates of NFIX mineral media possessing or lacking 1.5 g/L (28 mM) NH4Cl and solidified with 0.5% gellan gum were inoculated with S. thermoautotrophicus H1 or UBT1 and incubated in the presence or absence of 2.5% 15N2 for 3 days. Then biomass was collected, dried at 80 °C for 12 hours and analyzed at the MBL Stable Isotope Laboratory (Woods Hole, MA) on a Europa 20-20 CF isotope ratio mass spectrometer equipped with a Europa ANCA-SL element analyzer. A. vinelandii was grown in suspension in Burk's medium possessing or lacking 1.5 g/L NH4Cl, with stirring, at 30 °C for 3 days.
At the RWTH Aachen: 15N2 gas was acid-washed for one hour. Plates of NFIX mineral media lacking 1.5 g/L (28 mM) NH4Cl and solidified with 1.0% gellan gum were inoculated with S. thermoautotrophicus H1 or UBT1 and incubated for five days in the presence of 10% 15N2 gas. Then biomass was collected, dried at 80 °C overnight and sent for analysis to ISO-analytical, Crewe, UK.
At Universidad Nacional de Rosario: 15N2 gas was acid-washed for 30 minutes. Plates of NFIX mineral media possessing or lacking 1.5 g/L (28 mM) NH4Cl, fortified with 0.5% activated charcoal and solidified with 1.5% agar were inoculated with S. thermoautotrophicus H1 or UBT1 and incubated at 60 °C for 5 days in desiccators in the presence or absence of 1.5% 15N2. A. vinelandii DJ and DJ3357 (nifD− nifK−)were grown in Burk's medium with (DJ33) or without (DJ) 1 g/L NH4 Cl for 3 days at 28 C in desiccators in the presence or absence of 0.75% 15N2. Then biomass was collected, samples were dried at 60 °C for 24 hours and analyzed at the MBL Stable Isotope Laboratory (Woods Hole, MA) on a Europa 20-20 CF isotope ratio mass spectrometer equipped with a Europa ANCA-SL element analyzer.
At Michigan State University: 15N2 gas was acid-washed for 24 hours. Plates of NFIX mineral media possessing or lacking 1.5 g/L (28 mM) NH4Cl were solidified with 1% gellan gum with NH4Cl and 6 plates without NH4Cl were each inoculated with UBT1, H1 and P1-2 strains. These plates were incubated for 3 days in 40% H2, 10% CO2 and 50% air atmospheres with 2% of the atmosphere replaced with acid-washed 15N2. We also incubated plates of Azotobacter vinelandii (wild type) and A. vinelandii (nifD- mutant), which cannot fix nitrogen in Mo-containing media, as positive and negative controls, respectively. Controls were incubated on mineral media with 1% glucose either with (A. vinelandii nifD−) or without (A. vinelandii WT) NH4Cl in the presence of an air atmosphere with ~2% of the atmosphere replaced with acid-washed 15N2. After incubation, growth was scraped from plates and washed in Phosphate buffered saline to remove potential contaminating nitrogen. Two NH4-free plates worth of biomass were pooled into single samples at this point to compensate for poor growth of H1, UBT1 and P1-2 strains on these media. Biomass was dried at 60 °C for 24 hours, then analyzed for 15N content on a GV instruments Isoprime Mass Spectrometer interfaced with a EuroVector Elemental Analyzer 3000 Series.
Sequencing and bioinformatic analyses
At Universidad Nacional de Rosario: genomic DNA from S. thermoautotrophicus UBT1 (Rosario), was extracted with cetyltrimethylammonium bromide58. DNA was fragmented by nebulization, ends repaired, adaptors ligated and small fragments removed following GS-FLX Titanium library preparation. Libraries were sequenced with single-end FLX 454. The reads were assembled with Newbler v.2.5.359 and the genome annotated by RAST.
At the RWTH Aachen: genomic DNA from S. thermoautotrophicus UBT1 (Aachen), was prepared with a NucleopSpin Tissue kit (Macherey-Nagel, Germany). DNA was fragmented using a Biorupter Pico (Diagenode, Belgium) and libraries were prepared with the TruSeq kit. Libraries were sequenced with the 250PE reagent kit on a MiSeq (Illumina). PacBio reads were generated by GATC (Germany). Illumina reads were trimmed for quality with Trimmomatic (V0.32)60 and Illumina and PacBio reads were assembled with SPAdes version 2.5.161. The resulting assembly was annotated with RAST.
At Harvard Medical School: genomic DNA from S. thermoautotrophicus strain H1 was isolated with the DNeasy blood and tissue kit (Qiagen). Quality was verified with Bioanalyzer chips (Agilent). DNA was fragmented by adaptive focused acoustics (Covaris) to 400 bp or 3 kb and libraries prepared with the TruSeq kit. Libraries were sequenced with 250 PE reagent kits on a MiSeq (Illumina). PacBio reads were generated with the SMRTbell Template Prep Kit. Reads were trimmed for quality with Trimmomatic61 and assembled with SPAdes 3.161. The resulting assembly was annotated with RAST28, augmented with a bidirectional BLAST62 search against all bacterial protein sequences from the UniProt database63 to increase the number of genes with a putative function.
At Michigan State University: genomic DNA from H. schlegelii DSM2000 was obtained directly from DSMZ and was also extracted from H. schlegelii DSM2000 grown in Na-pyruvate mineral media (DSM 260: 4.5 g/L Na2HPO4, 1.5 g/L KH2PO4, 0.01 g/L MnSO4*7H2O, 0.2 g/L MgSO4*7H2O, 0.01 g/L CaCl2*2H2O, 0.005g/L Ferric citrate, 1.0 g/L NH4Cl,1.50 g/L Na-pyruvate and 3.0 mL DSM trace element solution 6) without shaking at 60 °C. Cells were pelleted, incubated for 30 minutes at 55 °C with 280 ul Tissue Cell Lysis solution (Epicentre) and with 20 ul Proteinase K (Roche). 200 ul MPC protein precipitation solution was then added, the supernatant was transferred to isopropanol and washed with 70% ethanol. Libraries were prepared for sequencing from both sources of DNA. Genomic DNA was sheared on a Covaris S2 ultrasonicator using the manufacturer's recommended parameters for shearing to 500 bp. Sheared DNA was end repaired, A-tailed and Illumina adapters were ligated using enzymatic kits from New England Biolabs64. Ligation products were amplified for 15 PCR cycles with Phusion polymerase (NEB) and size-selected on a 2% agarose gel. Finished libraries were pooled and sequenced on an Illumina MiSeq 150 bp paired sequencing run. Quality-filtered reads were assembled with the A5 pipeline65 and the resulting assembly was annotated with RAST.
The CoxMSL operon sequence from H. schlegelii DSM2000 was completed using the polymerase chain reaction (PCR). A DreamTaq kit (Thermo) was used to amplify for 35 cycles and the amplicon was gel-purified and sequenced on an ABI 3730XL with BigDye chemistry. A quality-trimmed Sanger read was used to merge the two scaffolds in Phrap64,66.
Phylogenetic analyses were performed on 16S rRNA genes by downloading sequences from the NCBI, RefSeq Targeted Loci Project (revised Nov. 2010 at http://www.ncbi.nlm.nih.gov/genomes/static/refseqtarget.html; date of access 23/08/2015). 352 sequences representing Actinobacteria with sequenced genomes available were retrieved, as well as B. subtilis as an outgroup. These were aligned to the 16S sequences from the S. thermoautotrophicus genomes using Clustal Omega version 1.2.167 with default parameters and 30 iterations of sequence input order. The resulting alignment was submitted to the Gblocks server68 and processed with the default settings. The consolidated alignment was used to form a distance matrix and a neighbor-joining tree with 100 bootstrap replicates, which were then used to form a consensus tree; these operations were carried out with PHYLIP version 3.69569. The final tree was edited in MEGA670: for the condensed tree, branches that were monophyletic according to their current classification in NCBI Taxonomy were collapsed up to the family level.
Phylogenetic analyses were performed on protein sequences by identifying highly conserved sequences as previously described28. The subset of these represented in both the H1 and UBT1 genomes, including 14 ribosomal proteins and a phosphatidate cytidylyltransferase, were identified. Subsequently all Actinobacterial sequences, as well as B. subtilis as an outgroup, of the corresponding protein families were downloaded from the PFAM server71. These sequences were combined with the S. thermoautotrophicus proteins and the proteins from each family were concatenated by species to form a single extended protein sequence representing a single isolate. The resulting table contained 431 strains, including H1 and UBT1. These were aligned in Clustal Omega with 30 iterations of input order, the resulting alignment trimmed with Gblocks, the trimmed alignment used to form a distance matrix and neighbor joining tree with 100 bootstrap replicates and the consensus tree all generated with PHYLIP as described above. The final tree was edited in Mega to collapse monophyletic branches to the family level, excepting the immediate neighbors of H1 and UBT1.
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DCM was supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number F32GM105122. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. JWM was supported for part of this work by a BBSRC David Phillips Fellowship (BB/F023308/1). "Nitrogen Ideas Lab: Oxygen-Tolerant Nitrogenase" (BB/L011468/1, NSF 1331218) supported LL, MLF, JSN, JWM and AWR. LL was also supported by Agencia Nacional de Promoción Científica y Tecnológica from Argentina. RLC was supported by Gordon and Betty Moore Foundation GBMF 2550.04 Life Sciences Research Foundation postdoctoral fellowship. We wish to thank many members of the nitrogenase community for helpful discussions.
Harvard Medical School, 200 Longwood Ave, Boston, 02130, MA
Drew MacKellar, Roger L. Chang, Tyler J. Ford & Pamela A. Silver
Wyss Institute, 3 Blackfan Cir, 02115, Boston, MA
Drew MacKellar, Peter Q. Nguyen, Neel S. Joshi & Pamela A. Silver
Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Zavalla, Santa Fe, S2125ZAA, Argentina
Lucas Lieber & Hugo R. Permingeat
Dept. Life Sciences, Imperial College, London, SW7 2AZ, UK
Lucas Lieber, James W. Murray & Alfred W. Rutherford
Department of Plant Biology, Michigan State University, 612 Wilson Rd, East Lansing, 48824, MI, USA
Jeffrey S. Norman, Jimmy Woodward & Maren L. Friesen
Institute for Biology I, BioSC, RWTH Aachen University, Worringer Weg 3, 52074, Aachen, Germany
Anthony Bolger, Mehtap Oksaksin, Björn Usadel & Jürgen Prell
TheLab Inc, 1340 E. 6th Street Suite 603, Los Angeles, 90021, CA, USA
Cory Tobin
Drew MacKellar
Lucas Lieber
Jeffrey S. Norman
Anthony Bolger
James W. Murray
Mehtap Oksaksin
Roger L. Chang
Tyler J. Ford
Peter Q. Nguyen
Jimmy Woodward
Hugo R. Permingeat
Neel S. Joshi
Pamela A. Silver
Björn Usadel
Alfred W. Rutherford
Maren L. Friesen
Jürgen Prell
D.M., L.L., J.S.N., M.O. and J.P. have done the majority of the lab work. C.T. isolated and donated the H1 strain. R.L.C., T.J.F. and J.W. have done supportive analysis. D.M., P.Q.N., A.B. and J.W.M. have done genetic analysis and bioinformatics work. D.M. has written the manuscript with the help of M.L.F., J.W.M. and J.P., H.R.P., N.S.J., P.A.S., B.U., M.L.F. and A.W.R. have organized the funding.
Supplementary Figures 1 to 3
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
MacKellar, D., Lieber, L., Norman, J. et al. Streptomyces thermoautotrophicus does not fix nitrogen. Sci Rep 6, 20086 (2016). https://doi.org/10.1038/srep20086
DOI: https://doi.org/10.1038/srep20086
Transcriptomics differentiate two novel bioactive strains of Paenibacillus sp. isolated from the perennial ryegrass seed microbiome
Tongda Li
Ross Mann
Timothy Sawbridge
Scientific Reports (2021)
Are we there yet? The long walk towards the development of efficient symbiotic associations between nitrogen-fixing bacteria and non-leguminous crops
Vânia C. S. Pankievicz
Thomas B. Irving
Jean-Michel Ané
BMC Biology (2019)
The microbial nitrogen-cycling network
Marcel M. M. Kuypers
Hannah K. Marchant
Boran Kartal
Nature Reviews Microbiology (2018)
Plant growth promotion by streptomycetes: ecophysiology, mechanisms and applications
Jucimara Anunciação de Jesus Sousa
Fabio Lopes Olivares
Chemical and Biological Technologies in Agriculture (2016)


text:
Q: retrieving parameters from html to a java email file I am creating a contact form that allowing the user to input their information and an email would be sent to the designated email assigned. I have been having trouble figuring out to retrieve the parameters of the html file and attaching it to the email components. For example I want the subject of the email to be the persons name and the message to come from the contact form message.
-This is my java code
public class Main {

private static String USER_NAME = "cakesbyluckydemo";
private static String PASSWORD = "cakedemo";
private static String RECIPIENT = "cakesbyluckydemo@gmail.com";

public static void main(String[] args) {
    String from = USER_NAME;
    String pass = PASSWORD;
    String[] to = { RECIPIENT };
    String subject = "Java send mail example";
    String body = "Welcome to JavaMail!";

    sendFromGMail(from, pass, to, subject, body);
}

private static void sendFromGMail(String from, String pass, String[] to, String subject, String body) {
    Properties props = System.getProperties();
    String host = "smtp.gmail.com";
    props.put("mail.smtp.starttls.enable", "true");
    props.put("mail.smtp.host", host);
    props.put("mail.smtp.user", from);
    props.put("mail.smtp.password", pass);
    props.put("mail.smtp.port", "587");
    props.put("mail.smtp.auth", "true");

    Session session = Session.getDefaultInstance(props);
    MimeMessage message = new MimeMessage(session);

    try {
        message.setFrom(new InternetAddress(from));
        InternetAddress[] toAddress = new InternetAddress[to.length];

        for( int i = 0; i < to.length; i++ ) {
            toAddress[i] = new InternetAddress(to[i]);
        }

        for( int i = 0; i < toAddress.length; i++) {
            message.addRecipient(Message.RecipientType.TO, toAddress[i]);
        }

        message.setSubject(subject);
        message.setText(body);
        Transport transport = session.getTransport("smtp");
        transport.connect(host, from, pass);
        transport.sendMessage(message, message.getAllRecipients());
        transport.close();
    }
    catch (AddressException ae) {
        ae.printStackTrace();
    }
    catch (MessagingException me) {
        me.printStackTrace();
    }
}

}
-This is some of my html code
 <form>

      Name

        <input class="input" type="text" placeholder="e.g Alex Smith">

      Email

        <input class="input" type="email" placeholder="e.g. alexsmith@gmail.com">

      Message

        <textarea class="textarea" placeholder="Textarea"></textarea>

        <button class="button is-link">Submit</button>

  </form>

A: You need a servlet that will handle the form submission.
In the servlet you can retrieve your form values and call your sendFromGMail method.

A: For sending email from java web application, you can use Servlet so that you call submit your input form.
Let's see an example:
Your html input form would be like:
<!DOCTYPE html>

  <title>Email Example</title>
  <meta charset="utf-8">
  <meta name="viewport" content="width=device-width, initial-scale=1">
  <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/css/bootstrap.min.css">

<form action="servlet/SendEmail" method="post">

      Name:
      <input type="text" class="form-control" id="name" placeholder="Enter name" name="name">

      Email:
      <input type="email" class="form-control" id="email" placeholder="Enter email" name="email">

        Message Body:
        <textarea class="form-control" rows="5" cols="100" placeholder="Enter your message" name="message"></textarea>

    <button type="submit" class="btn btn-default">Submit</button>

  </form>

Your java class which will extends servlet class would be like:
import java.io.*;
import javax.servlet.ServletException;
import javax.servlet.http.*;
import java.util.Properties;
import javax.mail.*;
import javax.mail.internet.*;

public class SendEmail extends HttpServlet {
@Override
public void doPost(HttpServletRequest request, HttpServletResponse response)
            throws ServletException, IOException {

response.setContentType("text/html");
PrintWriter out = response.getWriter();

String name=request.getParameter("name");
String toEmails=request.getParameter("email");
String message=request.getParameter("message");

String subject = "Test email";

try{
    System.out.println("Data: \n "+"Name: "+name+" email: "+toEmails+" message: "+message);
    sendFromGMail("youremail@gmail.com", "yourPassword", toEmails, subject, message);
    out.print("Message sent successfully");
}catch (Exception e2) {
    System.out.println(e2);
    out.print("Message cannot send! Check setting");
}

out.close();
}

public void sendFromGMail(final String from,final String password,String to,String sub,String msg){
          //Get properties object
          Properties props = new Properties();
          props.put("mail.smtp.host", "smtp.gmail.com");
          props.put("mail.smtp.socketFactory.port", "465");
          props.put("mail.smtp.socketFactory.class",
                    "javax.net.ssl.SSLSocketFactory");
          props.put("mail.smtp.auth", "true");
          props.put("mail.smtp.port", "465");
          //get Session
          Session session = Session.getDefaultInstance(props,
           new javax.mail.Authenticator() {
           @Override
           protected PasswordAuthentication getPasswordAuthentication() {
           return new PasswordAuthentication(from,password);
           }
          });
          //compose message
          try {
           MimeMessage message = new MimeMessage(session);
           message.addRecipient(Message.RecipientType.TO,new InternetAddress(to));
           message.setSubject(sub);
           message.setText(msg);
           //send message
           Transport.send(message);
           System.out.println("message sent successfully");
          } catch (MessagingException e) {throw new RuntimeException(e);}

    }

}

And you web.xml file will be:
<?xml version="1.0" encoding="UTF-8"?>
<web-app version="2.5" xmlns="http://java.sun.com/xml/ns/javaee" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://java.sun.com/xml/ns/javaee   http://java.sun.com/xml/ns/javaee/web-app_2_5.xsd">
    <servlet>
        <servlet-name>SendEmail</servlet-name>
        <servlet-class>com.notearena.emailwebapp.SendEmail</servlet-class>
    </servlet>
    <servlet-mapping>
        <servlet-name>SendEmail</servlet-name>
        <url-pattern>/servlet/SendEmail</url-pattern>
    </servlet-mapping>
    <welcome-file-list>
        <welcome-file>index.html</welcome-file>
    </welcome-file-list>
</web-app>

You may find a complete example here:
https://github.com/Md-MamunAbdulKayum/Email-java-web-app-/tree/master

redpajama_set_name: RedPajamaStackExchange


text:
Fello Star de Labé – gwinejski klub piłkarski, grający w pierwszej lidze gwinejskiej, mający siedzibę  w mieście Labé.

Historia
Klub został założony w 1988 roku. Swój pierwszy sukces osiągnął w 2000 roku, gdy zdobył Puchar Gwinei. W finale pokonał 2:1 zespół Horoya AC. W 2004 roku został po raz pierwszy mistrzem Gwinei. Tytuł mistrzowski przyznawano wówczas zdobywcy krajowego pucharu. W jego finale Fello Star pokonał po serii rzutów karnych CI Kamsar (po 120 minutach był remis 2:2). Kolejne tytuły mistrzowskie Fello Star wywalczył w latach 2006, 2008, 2009 i 2010.

Stadion
Swoje domowe mecze klub rozgrywa na stadionie o nazwie Stade Régional Saifoullaye Diallo w Labé. Stadion może pomieścić 5000 widzów.

Sukcesy
Guinée Championnat National:
 mistrzostwo (5): 2004, 2006, 2008, 2009, 2010
 wicemistrzostwo (2): 2005, 2011

Puchar Gwinei:
 zwycięstwo (2): 2000, 2004

Superpuchar Gwinei:
 zwycięstwo (2): 2007, 2009

Występy w afrykańskich pucharach

Reprezentanci kraju grający w klubie
Stan na wrzesień 2016.

Przypisy

Gwinejskie kluby piłkarskie
Kluby piłkarskie założone w 1988
Labé

redpajama_set_name: RedPajamaWikipedia


text:
Exciting Year Ahead!!
Happy New Year!!!! 2015 is looking to be an exciting year ahead for Helen starting with a Richard Jones production of Hansel and Gretel and performances with Lesley Garrett in Chorus! for the spring season at WNO. Other highlights to look forward to include a return to The Royal Opera House Covent Garden in the summer and Sweeney Todd in the Autumn/Winter.
Click here to return to the News & Reviews page
Copyright © Helen Jarmany

redpajama_set_name: RedPajamaCommonCrawl


The Capsis Resort Crete located on a private peninsula has a private beach and direct access to the beautiful public beach where there are many good bars and restaurants. These hotels (Capsis Elite Resort, Classic Collection Hotels, Ruby Red Regal Hotel, Eternal Oasis Neighborhood, Capsis Resort, Exclusive Collection Hotels, Oh! All Suite Hotel, Divine Thalassa Neighborhood and the Crystal Energy Hotel.) have a host of different room types to suit any party and the villas are stunning with the views from many of the rooms being simply perfect.


There is, of course, the slim chance that Nash, Rembrandt, Van Gogh, Goya, Picasso, Gauguin and all the other fierce pacifists of art were wrong about war and Blair is right. When the chronicles of humanity reach their final page, it could be that Blair v Goya or Blair v Picasso would end in a big Blair victory. But somehow I don't see it. Paul Nash, the greatest war artist this country has ever produced, saw absolutely clearly what politicians and spinmeisters lose sight of as soon as the chauffeur-driven Jag turns up at their door: which is that life is holy and that no government in any situation ever has the right to waste it.
Anyway, enough of this naivety and impracticality. Let us re-adopt the sensible modern exhibition-strolling mode, dismiss the emotive flounderings of those eminent war artists, wipe the tears from our eyes, and re-begin our admiration of Paul Nash with our wits about us.
Nash was the son of a barrister, born in 1889 in Kensington, of all places. His family had plenty of country connections, though, and young Paul spent enough weekends and holidays away from the smoke to know which side of the modern household coin he preferred. Being born in the city, he valued the landscape more actively than most country folk do. There is so much eagerness in his first landscapes. He had a thing about trees. Each tree, he insists, in one of the extremely helpful boxes of information dotted about the show, is as obvious an individual as any human. Botanically incorrect, no doubt, it's an extremely helpful viewpoint from which to draw elms. In large, bold watercolours, Nash's elms grow into strange and distinctive triffids, billowing up and out into amazing umbrella silhouettes.
Scientists will balk at his outrageous anthropomorphism, but it led to exciting art and some of the most interesting trees you will ever see.
Also worth noting in the opening room, and throughout, is the telling absence of humans. Gesturing stick-people pop up here and there to give an image scale or a sense of mystery, but they are rare. In general, Nash keeps us out of his pictures and lets the natural world do all the emoting. This absence of humans casts a curious shadow of privacy across the whole show and gives you the feeling that Nash and nature are lovers enjoying a secret tryst, and that the rest of us shouldn't be there. I've been making a film about Van Gogh and he too went into these kinds of private ecstasies before nature. The sensations he captured are almost sexual. The ecstatic rushes of feeling conveyed in Nash's landscapes certainly have an orgasmic quality, too.
While the exciting opening gallery teaches you how to look at English nature, the war room that follows beats you up and devastates you. Nash enlisted in the Artists' Rifles in December 1914 and was sent to the front at Ypres. Invalided out after he fell into a trench, he returned as an official war artist just in time to record the aftermath of Passchendaele, at which 310,000 British casualties were counted. Nash, in his second greatest picture, the huge view of The Menin Road, painted in 1919, the Imperial War Museum's most definitive possession, doesn't show a single one of them, yet he achieves an image of the conflict so horribly visceral that it dumps you trembling at the front line.
It's the absence of people — of corpses — that is once again his masterstroke. If you look closely, there are a few scampering soldiers discernible among the bomb craters, but they're tiny. All the big feelings here are conveyed by the dead mud, the broken trees, the smashed machinery, the squalid pools of grey water, the frozen explosions, the aggressive arc lights cutting the sky to shreds. Something mightier than any human corpse has been killed here: hope.
English painting may not have contributed much that is crucial to the history of 20th-century art, but in Nash's depictions of the obscenity of war it has only one real equal, and that is Picasso's Guernica. That's how important The Menin Road is. Yet, remarkably, one world war later, Nash went on to better it.
There was, at least, a decent interlude. In 1920, he holidayed in the dank-looking Kentish seaside resort of Dymchurch and produced a memorable series of empty coastal landscapes, in which two types of harshness, nature's and man's, are simultaneously admired. Later in the 1920s and, alas, for most of the 1930s, he took up surrealism and was very bad at it. He could do implicit so brilliantly, yet insisted, in terrible picture after terrible picture, in attempting to be surrealistically explicit. Thus a giant peregrine falcon comes across a full-size mirror propped up on a coastal headland and stops to stare into it meaningfully, as if amazed by his own powers of flight. Ouch.
This clunky surrealism is so bad that it makes his superior response to the second world war even more remarkable. He was once again made an official war artist, the only such artist to serve in both world wars, and like a great boxer with one winning effort left in him, Nash roused himself splendidly for the final round. This time it was the battle in the skies that fascinated him, and he produced a spooky series of English horizons crisscrossed by vapour trails and explosion clouds, as British guns and German planes played noughts and crosses in the heavens. In 1941, he painted his most memorable war image, Totes Meer, a metal sea of broken enemy planes, washed up on an English beach, in which the scattered insignia of the Luftwaffe, black crosses on white backgrounds, stand in for the tombstones in a graveyard.
There isn't as much raw horror in Nash's second stab at being a war artist. He's been there and done that, and this time is cool enough to observe a mad and im-moral beauty in the crisscrossing vapour trails and the multicoloured airbound explosions. Two different wars. Two types of indictment. A rare career. A great show.


Each year students in elementary, middle school and high school are required to complete summer reading assignments as a way to stay engaged with the reading process when away from school. Students will choose books from the approved lists and complete the assignment as instructed.
In lieu of reading the required novels, students in middle school and high school can choose to participate in the Knotts House Museum Summer Poetry to Last a Lifetime workshop. Parents can contact Lydia Nabors, Museum Educator at 850.922.2459 or Lydia.Nabors@dos.myflorida.com.


Male Integrated science Nigeria Limited is an indigenous borehole drilling company registered with corporate Affair Commission (C.A.C). With the current increased urbanization and incessant climant condition, water level is drying up while abstracted aquifers are not replenished fast, therefore Borehole drilling requires experts to manage for sustainability.
b. Payment of commitment fee (Hydro Geophysical Prognosis).
Trained Geologists supervise your drilling projects from design to handing over.
iii. Terrain Differences: Alluvial or basement.
v. Distance from our location. Ibadan, Lagos, Kano, Bayelsa, e.t.c.
vi. Quantity of boreholes to be drilled.
vii. Purpose for which it will serve: Hand Pump, Motorized or solar.
1. Make sure the borehole is operated for at least 10minutes everyday.
2. Design your chamber in a manner that no water from the surface infiltrates into the arena.
3. Provide a regulatory device and/or voltage regulator which handles the operation of the pump to prevent direct voltage on the pump.
4. Rejuvenate (flush) your borehole for at least 2 times in a year.
At MIS, we design sustainable borehole for your Domestic, Agricultural or Industrial use.
Until Hydro-geophysical prognosis is carried out, it wil be difficult to determine what quantity of water is trapped in the aquifer. More so, after the prognosis, the positivity of getting water on your proposed site can only be determined by our reliable equipment and not the volume.
1. Characteristics of rock representing the bedrock.
We give 2 years guarantee on the Hardware aspect of the borehole while the software aspect is the responsibility of the owner. (check factors affecting pricing of borehole) we appreciate your audience and your choice to doing business with us despite having several options.
Project takes minimum of 2 weeks from from Reconnaissance to handing over. Except on cases where it is prolonged to allow for good design, which we crave he indulgence of our client to deal with us as consultants and not a driller.


We cater for the diversity of students by providing a broad VELS based curriculum for Years 7 to 10.
We emphasise the development of numeracy, literacy and computer skills.
For many years, the wide range of co-curricular activities has been the strength of Ararat College.
Sport - House level, Wimmera, Western Victoria, State level.
Music - Instrumental music tuition, stage and brass bands.
College musical production - held annually since 1984.
Leadership - SRC, house captaincy, college production.
Camps and extended excursion programs from Years 7 to 12.
Outdoor education programs to develop initiative and self reliance.
Work Experience Program in Year 10.
Welfare program through pastoral care role of our chaplain.
The friendly and supportive learning environment is a valued feature of our college. Our teachers endeavour to make learning purposeful, challenging and relevant through individual and group work.
There is a quiet working atmosphere in our college where teachers seek to promote respect, openness and sensitivity of others in a caring positive environment that nurtures our students in forming appropriate values.


text:
Q: Scheme - using `this` as argument of a lambda function? Could someone please clarify the concepts behind this use of the "this" keyword?
(define call
  (lambda (obj method-name . args)
    (apply (obj method-name) obj args)))
(define -cuboid-
  (lambda (w l h)
    (define volume
      (lambda (this)
        (* h (call this 'area))))
    (define area
      (lambda (this)
        (* w l)))
    (lambda (method-name)
      (cond
        ((eq? 'volume method-name) volume)
        ((eq? 'area method-name) area)
        (else (error "method not found: ~s" method-name))))
(define r1 (-cuboid- 2 3 4))
(call r1 'area) ;=> 6
(call r1 'volume) ;=> 24

I understand that this is a keyword to refer to the object that is being used. I found out that this alone doesn't have any particular meaning in this program (it needs to refer to the arguments of the lambda functions).
The call is ((-cuboid- 2 3 4) 'volume), which brings to (* h (call this 'area)), where has this been defined?

A: this is simply the argument of the lambda, this could be anything; try changing it to, e.g., myself in the first lambda and me in the second (where it is not used, by the way, but needs to be there for the call to work).
The call to ((-cuboid- 2 3 4) 'volume) returns that procedure, with names bound according to the sketch below:
In call, r1 'volume calls the "lookup method" of -cuboid- and returns the volume procedure, which is then called with the obj argument, binding that to the name this
Thus, this gets bound to the r1 argument to call


text:
Neural Computation (2)
TocHeadingTitle
Filter by tocheadingtitle
Olivier Delalleau
Justifying and Generalizing Contrastive Divergence
Yoshua Bengio, Olivier Delalleau
DOI: https://doi.org/10.1162/neco.2008.11-07-647
FIGURES | View All (5)
We study an expansion of the log likelihood in undirected graphical models such as the restricted Boltzmann machine (RBM), where each term in the expansion is associated with a sample in a Gibbs chain alternating between two random variables (the visible vector and the hidden vector in RBMs). We are particularly interested in estimators of the gradient of the log likelihood obtained through this expansion. We show that its residual term converges to zero, justifying the use of a truncation—running only a short Gibbs chain, which is the main idea behind the contrastive divergence (CD) estimator of the log-likelihood gradient. By truncating even more, we obtain a stochastic reconstruction error, related through a mean-field approximation to the reconstruction error often used to train autoassociators and stacked autoassociators. The derivation is not specific to the particular parametric forms used in RBMs and requires only convergence of the Gibbs chain. We present theoretical and empirical evidence linking the number of Gibbs steps k and the magnitude of the RBM parameters to the bias in the CD estimator. These experiments also suggest that the sign of the CD estimator is correct most of the time, even when the bias is large, so that CD- k is a good descent direction even for small k .
Learning Eigenfunctions Links Spectral Embedding and Kernel PCA
Yoshua Bengio, Olivier Delalleau, Nicolas Le Roux, Jean-François Paiement, Pascal Vincent ...
Neural Computation (2004) 16 (10): 2197–2219.
DOI: https://doi.org/10.1162/0899766041732396
In this letter, we show a direct relation between spectral embedding methods and kernel principal components analysis and how both are special cases of a more general learning problem: learning the principal eigenfunctions of an operator defined from a kernel and the unknown data-generating density. Whereas spectral embedding methods provided only coordinates for the training points, the analysis justifies a simple extension to out-of-sample examples (the Nyström formula) for multidimensional scaling (MDS), spectral clustering, Laplacian eigenmaps, locally linear embedding (LLE), and Isomap. The analysis provides, for all such spectral embedding methods, the definition of a loss function, whose empirical average is minimized by the traditional algorithms. The asymptotic expected value of that loss defines a generalization performance and clarifies what these algorithms are trying to learn. Experiments with LLE, Isomap, spectral clustering, and MDS show that this out-of-sample embedding formula generalizes well, with a level of error comparable to the effect of small perturbations of the training set on the embedding.