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Antimicrobials, Resistance and Chemotherapy
This article is part of the Research Topic
Low-dose antibiotics: current status and outlook for the future View all 20 Articles
Benefits of Antimicrobial Drugs
Risks of Antimicrobials
Conclusion and Perspectives
Front. Microbiol., 12 June 2014 | https://doi.org/10.3389/fmicb.2014.00288
Benefits and risks of antimicrobial use in food-producing animals
Haihong Hao1, Guyue Cheng1, Zahid Iqbal1, Xiaohui Ai2, Hafiz I. Hussain1, Lingli Huang1, Menghong Dai1, Yulian Wang3, Zhenli Liu3 and Zonghui Yuan1,3*
1MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, China
2Yongtgz River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, China
3National Reference Laboratory of Veterinary Drug Residues and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan, China
Benefits and risks of antimicrobial drugs, used in food-producing animals, continue to be complex and controversial issues. This review comprehensively presents the benefits of antimicrobials drugs regarding control of animal diseases, protection of public health, enhancement of animal production, improvement of environment, and effects of the drugs on biogas production and public health associated with antimicrobial resistance. The positive and negative impacts, due to ban issue of antimicrobial agents used in food-producing animals, are also included in the discussion. As a double-edged sword, use of these drugs in food-animals persists as a great challenge.
Over the decades, world population tremendously increased to 7 billion (Food and Agriculture Organization [FAO], 2012) and 870 million (12.5%) of them were estimated to be undernourished during 2010–2012 (Food and Agriculture Organization [FAO], 2012; Krehbiel, 2013). Demand for animal source food tends to be soaring day by day, especially in developing countries (Food and Agriculture Organization [FAO], 2009; Krehbiel, 2013). It is acknowledged that the antimicrobial use is one of the most successful chemotherapies. Various antimicrobials have made significant contribution for the prevention, control, and treatment of infectious diseases in animals since 1940s (Forman and Burch, 1947). Low and sub-therapeutic dose of antimicrobials plays very important role for the improvement of feed efficiency, promotion of animal growth, and prevention and control of the diseases (Dibner and Richards, 2005; Niewold, 2007). International market value of veterinary drugs (including antimicrobials) tremendously increased from $8.65 billion in 1992 to $20.1 billion in 2010 and in 2018, it is expected to increase to $42.9 billion (GIA, 2012; Reports, 2012).
It is undeniable that rational use of antimicrobials plays a vital role in the production of food animals and protecting public health, while irrational and irresponsible use may cause antimicrobial resistance. On the basis of "Swan Reports" in 1969, Great British took first action for the restriction of antibiotics, being used in animals or capable of cross-resisting with antibiotics used in human medicine. In 1973, the European Community (EC) commenced the withdrawal of some important antibiotic use as growth promoters in animal feed. After that, Sweden banned the use of all growth-promoting antibiotics in 1986. Avoparcin, bacitracin, spiramycin, tylosin, and virginiamycin were withdrawn as growth promoters in the European Union (EU) from 1995 to 1999, on the basis of precautionary principle. In 2006, all the uses of low-dose antibiotics (5~40 ppm) in food animals, including flavomycin, avilamycin, salinomycin, monensin, and other animal-specific antibiotics, were banned in the EU with the intention to avoid their negative impact of resistant development (EPC, 2005; Marshall and Levy, 2011).
The benefits and risks of antimicrobials continue to be complex and controversial issues. The risks of antimicrobial drugs to public health associated with antimicrobial resistance raised great concern recently, while the benefits of antimicrobial drugs, such as prevention and treatment of animal diseases, protection of public health, enhancement of animal production, and improvement of environment, were disregarded most of the time. Many benefit-related claims have not yet been fully demonstrated in large-scale trials, and other trials revealed that the overall impact of the short-term benefits was poorly described. This article presents the benefits and risks of antimicrobials drugs used in food animals and discusses the positive and negative effects of the ban on antimicrobial growth promoters.
Prevention and Treatment of Animal Diseases
With intensive animal production, bacterial and parasitic diseases became more and more frequent. According to an estimate, 80 types of bacteria, such as Escherichia coli, Salmonella, and Clostridium welchii, posed a serious threat to poultry industry. Mastitis, caused by Staphylococcus aureus in dairy animals, led to a loss of $2 billion/year in the United States of America (USA) and an average cost of €485/dairy cow in the EU during 2012 (Heikkila et al., 2012). Due to infection caused by Streptococcus pneumonia, morbidity and mortality rates in calves increased to 40 and 20%, respectively (Akkermans and Vecht, 1994; Vogel et al., 2001). More than 50% of aquatic animals were infected by bacteria each year (Scarfe et al., 2011). Vibrio vulnificus became a potential health hazard for aquatic animals and human beings (Yano et al., 2004).
Approximately, 2169 parasites including 203 protozoa, 373 trematodes, 150 tapeworms, 404 nematodes, and 1030 arthropods have been found in livestock and poultry in China. About 4–20 billion dollars/year (8.3% annual output of animal husbandry) were lost due to parasitic diseases caused by coccidia, nematodes, ticks, and others in USA (Krausse and Schubert, 2010). Acute outbreaks of chicken coccidiosis paid a loss of 42 million pounds annually in the United Kingdom (Benjamin Franklyn Kaupp, 2010). In China, poultry industry had to face billions of dollars annual loss due to almost 100% chicken morbidity by coccidiosis (Zhang et al., 2013). Sheep helminthiasis led to a loss of 2.22 million dollars annually in Australia (Hosking et al., 2009; Larsen et al., 2009).
Over one hundred of antimicrobials, including β-lactams, aminoglycosides, tetracyclines, amphenicols, macrolides, sulfonamides, fluoroquinolones, lincosamides, polypeptides, and polyene, have been used in food-producing animals around the world. These antimicrobials have played an essential role in the prevention, treatment, and control of food animal diseases caused by pathogens, such as pathogenic E. coli, S. aureus, S. pneumonia, Actinobacillus pleuropneumoniae, mycoplasma, Vibrio, and others (Hoflack et al., 2001; Krausse and Schubert, 2010). It was reported that in USA, 52.1% of total antimicrobials were used for the treatment of infectious diseases in animals, where 90% of starter pigs, 75% of grower pigs, 50% of finisher pigs, and 25~70% of cattle received the drugs through feed (Van Lunen, 2003; USFDA, 2009; GRACE, 2013). With a dose of 40 mg/kg, avilamycin in feed could remarkably decrease the incidence of diarrhea in post-weaning pigs (Partanen et al., 2007). When salinomycin was used in sows and pigs simultaneously, incidence of diarrhea in piglets was significantly reduced and the survival rate was increased by 13.95% (Nagaraja and Taylor, 1987). Sulfonamide and folic acid supplementation in diet increased live birth rate of piglets by 1% (Lindemann and Kornegay, 1989). Hence, it is concluded that the use of antimicrobials is a primary strategy for prevention and treatment of bacterial infections in food-producing animals.
Many antimicrobials have strong activity against parasites in animals. Use of sulfonamides in animals opened a new era of anti-parasitic drugs and made lots of parasitic diseases under control. Up till now, anti-parasitic drugs have shared about one-third sale of the global veterinary drug markets. Macrolides and benzimidazoles effectively controlled nematodes. Doramectin and ivermectin helped to prevent infection of Argulus siamensis in carp and Labeo rohita (Hemaprasanth et al., 2012). In rabbit, subcutaneous injection of ivermectin, at dose of 400 mg per kg, not only helped to clinical cure ear mite infection but also prevented loss of fur and thus, played a vital role for the improvement of fur production (McKellar et al., 1992).
Conclusively, due to unique advantages, such as exact targeting of pathogens, well-known mechanisms of activity and desired stability, antimicrobials justified their usage in livestock and poultry, and played important part for prevention and treatment of bacterial and parasite diseases.
Protection of Humans Against Zoonosis
Among animal infectious and parasitic diseases, more than 200 can affect human life. Campylobacter spp., Salmonella spp., E. coli O157, Vibrio parahaemolyticus, and Aeromonas hydrophila from animals pose great health threat to both humans and animals (Altwegg and Geiss, 1989; Mellata, 2013). The United States Centers for Disease Control and Prevention (US-CDC) estimated that there were about 76 million annual cases of food-borne illness in USA, including 325,000 hospitalizations and 5000 deaths (Mead et al., 1999a). Annual cases of Campylobacter spp., Salmonella spp., E. coli O157, and V. parahaemolyticus were 1,963,000, 1,332,000, 62,500, and 5000, respectively (Mead et al., 1999a, b).
To some extent, antimicrobial agents guaranteed human food security and public health by controlling animal diseases and preventing transmission of zoonotic pathogens from animals to humans. When added to animal feed or drinking water, these drugs could significantly decrease the bacterial contamination in animal products. For examples, virginiamycin decreased the contamination of Clostridium perfringens, Campylobacter spp., and other food-borne pathogens in animal carcasses (Tice, 2001; Russell, 2003; Hurd et al., 2005). Salinomycin reduced infection of type C Clostridium in sows and weaning piglets by 43% (Nagaraja and Taylor, 1987). Neomycin in animal feed significantly reduced the number of E. coli O157: H7 in animal feces, and gentamycin reduced bacterial count in poultry eggs and meat (Elder et al., 2002; Doyle and Erickson, 2006). When cattle was fed with neomycin sulfate for 48 h and held for 24-h drug withdrawal period before slaughtering, it shed considerably less E. coli O157:H7 cells than those pen mates who did not receive the treatment (Elder et al., 2002). A farm-level study in 2008 by Ohio State University demonstrated that only 39% of hogs, raised on conventional antimicrobial operations, were infected with Salmonella, while those were 54% in case of antimicrobial-free operations (Nunes, 2008; AMI, 2010). Florfenicol (10 mg/kg) presented 100% efficiency for the treatment of A. hydrophila of Piaractus mesopotamicus (Carraschi et al., 2012). Oxytetracycline hydrochloride or norfloxacin in bait feed reduced the number of A. hydrophila in water by 46.86~66.24%, indicating that the risk of the bacterial infection to humans be decreased.
Enhancement of Animal Production
In 1943, a few farmers in USA found that pigs fed with penicillin-fermented mixture grew faster (Wahlstrom et al., 1950; Hewes, 1955; Taylor and Gordon, 1955). In 1946, Moore found that low dose of streptomycin stimulated chick's growth (Moore and Evenson, 1946; Dibner and Richards, 2005). Subsequently, chlortetracycline, doxycycline, and sulfonamides helped growth promotion in calves, pigs, and chicken. Cunha from University of Florida and Stokstad from University of Washington reported that penicillin in fermentation mixture functioned as a growth promoter for food-animals (Cunha et al., 1951). Legal use of the antimicrobials in feed has a history of over 60 years. Food and Drug Administration in USA (US FDA) approved these drugs as growth promoters for animals in 1951. Till 1978, 47.9% of the antimicrobials were used to be added in animal feed in USA. About 60% of poultry, 93% of chicken, 97% of growing pigs, and 80% of fattening pigs received antimicrobials through diet during the early 1990s. More than 40% of the drugs were added in animal feed at subtherapeutic level for improving animal production in USA during 1990s (Van Lunen, 2003). With a substantial contribution to the development of food-animal production at global level, veterinary antimicrobials tend to be necessities to cope with increasing food demand for humans.
Role of antimicrobials for the improvement of feed conversion ratio (FCR), animal growth, and reproductive performance has been well proven as given in Table 1, and discussed under following points (Cromwell, 2002). (1) Orally administered antimicrobials in pigs increased diet digestibility and improved feed utilization efficiency by 1.7~5.1% and 6.9~7%, respectively (Cromwell, 1999; Hardy, 1999; JETACAR, 1999; Van Lunen, 2003). (2) Addition of the drugs (e.g., chlortetracycline, sulfonamide, folic acid, carbadox, tilmicosin, tylosin, or sulfamethazine) in feed could remarkably improve the conception rate, farrowing rate, milk secretion, productive efficiency of sow, and live birth rate of piglet (Soma and Speer, 1975; Lindemann and Kornegay, 1989; Alexopoulos et al., 1998; Kantas et al., 1998; Weber et al., 2001; Partanen et al., 2007). (3) Feeding antimicrobials to pigs increased their weight gain by 1.9~16.4% (Nagaraja and Taylor, 1987; Cromwell, 1999; JETACAR, 1999; Van Lunen, 2003; IFAH-EuroP, 2005). (4) Administration of antibiotics (bacitracin zinc, colistin sulfate, flavomycin, and florfenicol) in fish diet significantly improved the feed conversion and promoted their growth (He et al., 2011; Zhou et al., 2011). (5) Antimicrobial (tiamulin, nosiheptide, salinomycin, and tylosin) supplementation could also improve the carcass quality by decreasing the fat thickness and increasing the lean meat of food-producing animals (Cromwell et al., 1984a, b; Cromwell and Stahly, 1985; Lindemann et al., 1985; Van Lunen, 2003).
TABLE 1. Part of evidences for the role of antimicrobials on feed utilization, growth promotion, reproductive performance, and carcass quality.
A lot of studies were carried out to find the mechanism involved in beneficial aspects of antimicrobials in animals. Jukes, Franti, and other scientists proved that the drugs attenuated intestinal wall and improved the digestibility of nutrients (Manson, 1968; Falkow, 1970; Jukes, 1970; Franti et al., 1971, 1972; Dibner and Richards, 2005). Midtvedt (1986) and Norin (1997) confirmed that oral doses of antimicrobials improved the structure of intestinal flora (Midtvedt, 1986; Norin, 1997). Salinomycin and avilamycin in feed improved the bioavailability of α-tocopheryl acetate in broilers by altering lipid absorption (Knarreborg et al., 2004). According to a previous review by Allen et al. (2013), antimicrobials have multi-functional role in animals, elaborated under following points: (1) these could reduce the colonization of intestinal bacteria and inhibit the growth of pathogenic microorganisms; (2) by decreasing the thickness of mucous membrane, led to more absorption of nutrients and reduced fermentation; (3) they directly neutralized the host immune response. In short, antimicrobials could affect the host intestinal flora, intestinal physiology, and immune system, and consequently, prevent disease, improve feed conversion, and enhance the growth of animals (Niewold, 2007). Till now, there are no appropriate alternatives which can replace antimicrobial growth promoters, in case those remain banned. Although numerous feed additives, mainly pre- and pro-biotic products, are commercially available now and seem to have potential to replace these growth promoters, but their true efficacy and mechanism of action in domestic animals remain unclear because of some inconsistent experimental results (Gaggia et al., 2010; Allen et al., 2013). Additionally, lack of safety evaluation and poor stability also limited the practical use of pre- and pro-biotic as feed additives.
Improvement of Environment
According to a report of Center for Food Safety (CFS, 2013) and Food and Agriculture Organization (FAO, 2006), housing stress, due to over-crowding of animals, creates sweeping and devastating impacts on the natural and human environment leading to global warming, land degradation, air and water pollution, and loss of biodiversity. Livestock waste is one of the major sources of greenhouse gases, as the abnormal fermentation of gastrointestinal tract contents can produce lots of methane, ammonia, carbon dioxide, as well as stench gases (e.g., nitrate, ethylene acid, methyl mercaptan, hydrogen sulfide, methylamine, and trimethylamine). Fecal waste of animals generally contains 24% protein and 6.1~17.96% amino acids. Nitrogen and phosphorus in the waste lead to environmental pollution, water eutrophication and ecological imbalance.
Some antimicrobials in feed could inhibit the abnormal fermentation and consequently, reduce the emission of greenhouse gases (mainly CH4). For example, ionophores (monensin, lasalocid, and salinomycin), amoxicillin, ovoparcin, nigericin, or laidlomycin inhibited rumen microbial fermentation at different levels and thus, reduced the proportion of volatile fatty acids (VFA) and methane (Van Nevel and Demeyer, 1995; Fellner et al., 1997; Domescik and Martin, 1999). Since the mid-1970s, ionophorous antibiotics have been widely used as feed additives in ruminants due to their favorable effects on rumen fermentation and methane reduction (Kobayashi, 2010). Due to the efficacy and affordable price, ionophores have widely been used to reduce methane emission from livestock (Hook et al., 2010; Kobayashi, 2010). When ionophores (monensin and lasalocid) were mixed with rumen microorganisms in vitro, these inhibited methane by 50 and 44%, respectively and decreased NH3 by more than 50% (Russell and Jeraci, 1984). In rumen models, ionophores (monensin, lasalocid, and salinomycin) inhibited 10~20% lipolysis and biological hydrogenation (Van Nevel and Demeyer, 1977, 1995). The effects of the antibiotics on the abatement of methane production may be attributed to a selective antimicrobial action on rumen microbes (protozoa, ruminococci, streptococci, and lactobacilli). Addition of monensin and lasalocid in cow forage killed 82.5 and 76.8%, respectively, of the intestinal ciliated protozoa in rumen and hence, reduced the production of methane and VFAs (acetic acid and propionic acid) by ciliates (Guan et al., 2006). Generally, the gas production was reduced from 4 to 31% by monensin (Schelling, 1984; Rumpler et al., 1986; Kobayashi, 2010). A recent report has indicated that long-term administration of monensin in dairy cattle steadily reduced methane by 7% and this reduction persisted for 6 months with no adverse effect on milk yield (Odongo et al., 2007). However, previous studies also found that both, the methane level and protozoal number, returned to baseline after long-term administration of high concentration of the antibiotic (Guan et al., 2006; Odongo et al., 2007; Hook et al., 2010). The efficiency of monensin supplementation, for reducing methane output in ruminants, appeared to be different in the degree of abatement depending on the diet and animal used (Guan et al., 2006; Odongo et al., 2007; Hook et al., 2010). Effect of the drug on the methane levels in rumen was closely related to the ciliated, protozoal population. Microbial consortia, like protozoal population, in the ruminant gut may adapt to the antibiotics leading to the recovery of methane production yield, in case of a long-term usage. Therefore, the ciliates in the rumen may impact the outcome of antimicrobial supplementation, with adaptation being a possibility (Guan et al., 2006; Hook et al., 2010).
Through manure application, antibiotics got released into soil and could be absorbed by plants in arable land. Certain species of plants have the ability to bio-accumulate sulfamethoxine in their roots and stems, and this bioaccumulation was often higher in roots than in stems (Sarmah et al., 2006). Low concentrations of chlortetracycline and oxytetracycline in the soil media could markedly affect plant growth and development (Sarmah et al., 2006). However, there was a large variation in sensitivity among plant species to the soil used as the growth media (Sarmah et al., 2006).
When residues seeped into water, certain antimicrobials also played significant role in the prevention of water eutrophication for aquatic animals. For example, chlortetracycline, lomefloxacin hydrochloride, and sulfamethoxazole strengthened the absorption of nitrogen and phosphorus in water by aquatic plants, and chlortetracycline effectively removed 25% of water nitrate and nitrogen (Dodds et al., 1991; Jones, 2010). On the other hand, the presence of antibiotic residues in environment may cause some adverse impacts, like acute and chronic toxicity, during early life stages of different aquatic organisms (Halling-Sorensen et al., 2003; Sarmah et al., 2006).
Negative Impact of Antimicrobial Ban
Ban on addition of sub-therapeutic antimicrobials in feed appeared to result in a certain extent of recovery of some bacteria and some unintended impacts on animal health and welfare (Drouin, 1999; Rose et al., 1999; Lovland and Kaldhusdal, 2001; Jensen et al., 2003). Emergence of E. coli and Lawsonia intracellularis infection in post-weaning pigs was significantly increased and consequently, the morbidity and mortality due to diarrhea were considerably increased (Casewell et al., 2003; Dibner and Richards, 2005). In Denmark, the mortality rate in weaning piglets increased from 2.7% (before the ban) to 3.5% (after the ban) and the morbidity rate of enteric infections in post-weaning pigs increased by 600% (Casewell et al., 2003; Dibner and Richards, 2005). In Sweden, chronic infections due to E. coli and L. intracellularis became more common and the mortality in weaning pigs increased by 1.5% (Wierup, 2001; Casewell et al., 2003).
To control animal diseases and to keep animals healthy, more therapeutic antimicrobials had to be used after the ban. It was reported that the usage amount of therapeutic antimicrobials in Denmark increased by 33.6%, from 48 tons/year in 2001 to 125.5 tons/year in 2010 (DANMAP, 2010). The increased amount of therapeutic antimicrobials was equal to or even more than the total quantity of antimicrobials being used before the ban (Phillips, 1999; Casewell et al., 2003; Phillips et al., 2004a, b; Turnidge, 2004; DANMAP, 2010). As shown in Figure 1, therapeutic use of tetracycline, penicillins, and macrolides markedly increased from 28.5, 16.4, and 13.4 tons in 2001 to 35.55, 27.1, and 16.8 tons in 2010, respectively (Phillips, 2007; DANMAP, 2010).
FIGURE 1. Estimated total consumption (kg) of prescribed antimicrobial agents for production animals in Denmark (data obtained from DANMAP, 2010).
Withdrawal of low-dose antimicrobial use as feed additives may increase the level of pathogens such as Salmonella spp., Campylobacter spp., Clostridium and E. coli O157 in animal gut, boost the contamination of food and environment, and hence, enhance the opportunities for humans to be infected by these pathogens. Population of Campylobacter in broilers fed without antimicrobials was threefold higher than that in the broilers fed with any antimicrobials (Heuer et al., 2001). Incidence of food-borne Campylobacter in EU has been increased after the ban issue. Recovery of Clostridium from animal meat was also significantly increased in EU after its ban policy (Jones, 2000; Poduval et al., 2000). As a consequence, clostridial infections resulted in an outbreak affecting large human population in Demark and pointed out the high level of threat to public health (DANMAP, 2010). It is known that clostridial necrotic enteritis in animals is suppressed by some of the banned antimicrobials (e.g., virginiamycin). In the absence of these antibiotics, the bacterial population may increase in animal guts and colonization may lead to poor weight uniformity and fragile intestines in pigs and chicks. During food processing, infected animal carcasses could be the sources of contamination for food-borne pathogens and thereby, jeopardize food hygiene (Tice, 2001; Russell, 2003). Bacterial contamination of meat may, therefore, increase the risk of human infections. Contrary to EU, incidence of food-borne diseases in USA was declined by 23% in 1996 and among those, the infections due to Campylobacter and Salmonella were decreased by 30 and 17%, respectively. It was believed that ban of virginiamycin in USA might annually contribute to the death of 40,000 people, infected by Campylobacter (Cox, 2005).
The ban may lead to increased food-borne infections and elevated usage of therapeutic antimicrobials in both animals and humans. It is noteworthy that therapeutic use of antimicrobial agents in animals has a close relationship with the drugs used in humans with respect to the types of drugs used (Cook, 1999). The increased therapeutic use in animals may contribute to a worse, drug-resistance scenario both in animals and humans. It was noted that clinical isolates of vancomycin- or teicoplanin-resistant Enterococci from humans were very uncommon and the cases of quinupristin/dalfopristin-resistant Enterococcus faecium were very rare before the ban. Similarly, resistant E. faecium burden markedly increased and became a big challenge after the ban (Phillips, 2007).
After the European ban on growth-promoting antibiotics, it was found that FCR (total kg of feed used per grow out/total kg of live weight per grow out) in broiler was decreased by 0.016 kg/kg from November 1995 to May 1999 (1.78–1.796) in Denmark. Feed efficiency raised to a higher value of 1.83 immediately after the restrictions and more than 1.84 in late 1999 (Emborg et al., 2002; Dibner and Richards, 2005). Average daily gain of weaning piglets in Denmark was decreased from 422 g in 1995 to 415 g in 2001 (Casewell et al., 2003; Dibner and Richards, 2005). Production of broilers, cattle, and dairy cows was significantly decreased in 2006, as shown in Figure 2. In Sweden, weight gain of post-weaning piglets was reduced and feed costs were significantly increased after the abolishment of growth-promoting antimicrobial agents (Wierup, 2001; Casewell et al., 2003). Even after 10 years, aquaculture production in Sweden was unable to return to the past level (Casewell et al., 2003).
FIGURE 2. Production of food animals in Denmark from 1998 to 2010 (data obtained from DANMAP, 2010).
If the use of antimicrobials is banned in USA, FCR may decline by 5% and more than 1100 km2 area is required to plant corn and soybeans to meet the demand of feedstuff production. Consequently, required facilities for livestock and poultry production will be correspondingly increased by 100 million m2, and farming area will also be increased by 500 million m2 to obtain the current level of animal production (Casewell et al., 2003). It was estimated that withdrawal of antimicrobial growth promoters might lead to a loss of 5~10 or even 40 dollars/pig in USA (Matthews, 2001). It does not seem to be a high cost for the developed countries but what will be the consequences if these antimicrobials are banned worldwide? It was also investigated that 25% of the current poultry industry and $3 billions would be additionally required to attain the current annual animal production (Rodehutscord et al., 2002; IFAH-EuroP, 2005). In conclusion, keeping in view the ever-increasing world population and its demands, more food animals should be raised to meet the food supply demand in the case of growth-promoting antibiotics remain prohibited and this increased number of animals will again lead to the increase in greenhouse gas emission and deeper environmental pollution.
Inhibition of Biogas Production
Following the wide use of antimicrobial drugs in intensive animal production for growth promotion and prevention or treatment of disease, a large proportion of ingested drugs are excreted in manure and end up with livestock wastewater. Excreted antibiotics in the environment may partially inhibit methanogenesis in anaerobic waste-storage facilities, commonly used at Concentrated Animal Feeding Operation (CAFOs), and thus, decrease the rate at which bacteria metabolize animal waste products (Loftin et al., 2005; Sarmah et al., 2006).
During the anaerobic digestion of livestock waste, certain antimicrobials, including amoxicillin, aureomycin, oxytetracycline, thiamphenicol, florfenicol, sulfadimethoxine, and tylosin, had inhibitory effects on methane production (Lallai et al., 2002; Sun et al., 2009; Shi et al., 2011; Amin et al., 2012). However, no inhibitory effect but a stimulus for methane production was observed during anaerobic digestion of piggery wastewater in the presence of 10 mg/L florfenicol, amoxicillin, aureomycin, and sulfadimethoxine, while only the combination of high concentration of certain antimicrobials (130 mg/L florfenicol, 210mg/L amoxicillin, 10mg/L doxycycline, and 210 mg/L sulfadimethoxine) could decrease the methane production rate (Sun et al., 2009). Biogas volume, produced from per unit weight of biomass, was decreased with increasing concentrations of antibiotics, such as oxytetracycline, amoxicillin, and tylosin, and the inhibitory concentrations of oxytetracycline, amoxicillin, and tylosin were 8000, 9000, and 9000 mg/L, respectively (Amin et al., 2012). Only high concentration of thiamphenicol (160 mg/L), amoxicillin (120 mg/L), tetracycline (50 mg/L), and sulfamethoxydiazine (50 mg/L) had inhibitory effect on biogas production in the anaerobic digestion of pig waste slurry (Lallai et al., 2002; Shi et al., 2011). Actually, it is too difficult to attain those high concentrations of antibiotics in the excreta.
Antimicrobial Resistance Concerns
Misuse and overuse of antimicrobial may culminate in the development of drug-resistant pathogens resulting in poor response to treatment. Long-term and low-level exposure to antimicrobials may have greater selective potential than short-term and full-dose therapeutic use. A study observed that the percentage of tetracycline resistance genes in the fecal flora of conventionally raised feedlot steers was significantly higher than that in fecal samples from antimicrobial-free cattle (Harvey et al., 2009). Additionally, use of single antimicrobial may induce cross-resistance to antimicrobials used for animal and human medical therapy. For example, chlortetracycline use in growth rations was associated with ampicillin and tetracycline resistance in generic fecal E. coli, isolated from swine farms (Varga et al., 2009). Addition of chlortetracycline and sulfamethazine in cattle feed may be associated with higher prevalence (three to four fold greater than the control) of ampicillin- and tetracycline-resistant E. coli, isolated from the feces of treated animals (Alexander et al., 2010). Therefore, how to use antimicrobials, for effective treatment of bacterial and parasitic infections in food-producing animals, became the most important question for their use by avoiding the resistance development.
Regarding public health risk, more concern has been raised for the use of antibiotics in animals that may represent a potential threat to human health because the resistant pathogens in animals may transmit to humans and cause treatment failure of human medicines. A longitudinal study of the relationship between antimicrobial-resistant E. coli from human wastewater and swine fecal samples reported that the use of injectable (e.g., ceftiofur sodium) or oral (e.g., chlortetracycline) antibiotics may contribute to the high levels of E. coli resistance in swine and human isolates. Thus, slaughter plant workers may be at higher risk of carrying multidrug-resistant E. coli as compared to workers dealing with other animals (Alali et al., 2008). A study analyzed the correlation between E. coli isolates from human blood stream and food-producing animals (poultry, pigs, and cattle) for the prevalence of antimicrobial resistance in 11 countries during 2005–2008. Results revealed that there were strong and significant correlations between the strains from animals (especially poultry and pigs) and humans for resistance to multiple drugs (especially ampicillin, aminoglycoside, and fluoroquinolone; Vieira et al., 2011). However, there is not enough and direct evidence to support the hypothesis that a large proportion of resistant isolates in humans be derived from animal source foods. Some recent studies suggested that some resistant isolates from humans were more easily transmitted from companion animals (having close contact) or birds (by bird droppings especially during their migration; Haenni et al., 2012; Loncaric et al., 2013). The resistant bacteria may also be released into the environment by humans and then transferred into new hosts in the environment (Hower et al., 2013; Verkade and Kluytmans, 2013). Based on the results of some current studies, Dr. Hurt pointed out that public health risk due to infected animals need more attention than antibiotic resistance concern (Hurd et al., 2008, 2012). Therefore, further efforts are required for the risk assessment of antimicrobial use in food animals to check their potential impact on public health.
Positive Effects of Antimicrobial Ban
Aim for the withdrawal of antimicrobial agents used was to prevent humans and animals from drug resistance. The ban on antimicrobial growth promoters led to decreased drug resistance in some bacteria. For example, according to report from the DANMAP, substantial reductions (from 80 to 2%) in the prevalence of vancomycin-resistant enterococci (VRE) were observed after ban of avoparcin as the growth promoter during 1995 and 2010 (DANMAP, 2010). E. faecium and Enterococcus faecalis are two of the most common Enterococci species and in Europe, only vancomycin-resistant E. faecium (VREF) is highly prevalent in poultry (Werner et al., 2008). VREF was still present as a threat in the food chain even after 15 years of the EU ban on avoparcin and could be detected in 47% of the broiler feces with a selective enrichment method (Garcia-Migura et al., 2007; DANMAP, 2010).
The ban on tylosin as the growth promoter had a remarkable effect on the level of macrolide (erythromycin) resistance in Campylobacter coli (most common Campylobacter species in pigs) from pigs as it decreased from 66 to 20% in Denmark between 1998 and 2005 (Hammerum et al., 2007). However, DANMAP data showed that during 2006–2010, macrolide resistance in C. coli varied within the range of 10–20% without significant reduction (DANMAP, 2010). In contrast to EU, macrolides (e.g., tylosin) in USA had been approved for usage in food-producing animals as growth promoter for decades. Macrolide resistance in C. coli isolated from poultry, although higher than that in C. jejuni, has no significant change (5–20%) between 2002 and 2010.
Use of enrofloxacin in poultry was withdrawn by US-FDA in 2005 because it was supposed to induce fluoroquinolone resistance in Campylobacter and Salmonella from poultry and contribute to the antibiotic treatment failure in humans [Food and Drug Administration (FDA), 1998, 2002; Nelson et al., 2007]. After withdrawal of enrofloxacin from poultry, the rate of fluoroquinolone resistance in Campylobacter and Salmonella had been reduced in chicken during 2005–2007 (NARMS, 2010). Human clinicians also observed a reduction in domestically acquired Campylobacter and Salmonella infections with decreased susceptibility to fluoroquinolones, and it was thought to be a great achievement regarding public health (Nelson et al., 2007). However, the incidence rate of fluoroquinolone-resistant C. jejuni, from chicken breast, again increased (15.2~22.5%) in 2008–2010. Similarly, fluoroquinolone-resistant Campylobacter from broilers was also raised from 5.3 to 8.6% and from 0.8 to 7.7% in Denmark and Japan, respectively (CIPARS, 2008; JVARM, 2008; DANMAP, 2010; NARMS, 2010).
Definite targeting of pathogens, well-known mechanisms of activity, and preferable stability for administration are the unique advantages of antimicrobial use in food-animals for the prevention and treatment of bacterial and parasitic diseases, improvement of animal production performance, and protection of environment and public health. Withdrawal of antimicrobial use from food-producing animals may bring adverse effects on the production of food derived from animals and thus, on public health. EU banned only low-dose antibiotics (5~40 ppm) for their use as growth promoters in food animals. Until now, without helping for the cause, it led to some negative effects on food animal production and public health. What will happen if the antimicrobial agents are banned worldwide, especially in some developing countries with rapid increase in human population and food demand?
As a double-edged sword, non-rational uses of veterinary antimicrobials may result in pressure selection of antimicrobial resistant pathogens which may endanger both the animal and public health. Additionally, the presence of antibiotic residues in the environment, associated with overuse of antimicrobial drugs, may adversely influence the manure treatment systems by inhibition of biogas production. An economic analysis about use and withdrawal of antimicrobial growth promoters in USA revealed that the withdrawal may cause increased cost ($10/person) for food consumption and antibiotic-resistant infections cost the US healthcare system an excess of $20 billion ($60/person) annually (APUA, 2010). However, it is unknown that how much of these $20 billion is due to antimicrobial resistance associated with their use in food-producing animals.
Recently, the US FDA has also proposed restrictions on the antimicrobial growth promoters because some available information and evidences suggested that the sub-therapeutic use may increase the risk of antimicrobial resistance. To make wise strategies for controlling antimicrobial resistance and effectively respond to the public health concerns associated with drug resistance, FDA believes that it is very important and imperative to consider how antimicrobial drugs are being used and how to address their injudicious uses in nature. Only rational use and effective regulation can ensure a benefit–risk balance of the antimicrobial application in animal production. However, long-term policies will be required for the international regulation of antibiotic use in food-producing animals.
This article is supported by National Natural Science Foundation of China (31101856), Grants from National Basic Research program of China (2013CB127200), National Key Technology R&D Program (2012BAK01B00) and the research fund for young scholars in the Doctoral Program of Higher Education of China (2011014612003).
Akkermans, J. P., and Vecht, U. (1994). Streptococcal infections as cause of death in pigs brought in for necropsy. Tijdschr. Diergeneeskd. 119, 123–128.
Pubmed Abstract | Pubmed Full Text
Alali, W. Q., Scott, H. M., Harvey, R. B., Norby, B., Lawhorn, D. B., and Pillai, S. D. (2008). Longitudinal study of antimicrobial resistance among Escherichia coli isolates from integrated multisite cohorts of humans and swine. Appl. Environ. Microbiol. 74, 3672–3681. doi: 10.1128/AEM.02624-07
Pubmed Abstract | Pubmed Full Text | CrossRef Full Text
Alexander, T. W., Inglis, G. D., Yanke, L. J., Topp, E., Read, R. R., Reuter, T., et al. (2010). Farm-to-fork characterization of Escherichia coli associated with feedlot cattle with a known history of antimicrobial use. Int. J. Food Microbiol. 137, 40–48. doi: 10.1016/j.ijfoodmicro.2009.11.008
Alexopoulos, C., Tsinas, A., Kantas, D., Florou-Paneri, P., Read, M. P., Vassilopoulos, V., et al. (1998). A dose titration study on the effect of virginiamycin on specific blood parameters and milk quality in the sow. Zentralbl. Veterinarmed. 45, 535–542. doi: 10.1111/j.1439-0442.1998.tb00857.x
Allen, H. K., Levine, U. Y., Looft, T., Bandrick, M., and Casey, T. A. (2013). Treatment, promotion, commotion: antibiotic alternatives in food-producing animals. Trends Microbiol. 21, 114–119. doi: 10.1016/j.tim.2012.11.001
Altwegg, M., and Geiss, H. K. (1989). Aeromonas as a human pathogen. Crit. Rev. Microbiol. 16, 253–286. doi: 10.3109/10408418909105478
AMI. (2010). Antibiotic Use in Livestock Production: Ensuring Meat Safety, American Meat Institute. Available at: http://www.MeatAMI.com
Amin, M. M., Hashemi, H., Ebrahimi, A., and Ebrahimi, A. (2012). Effects of oxytetracycline, tylosin, and amoxicillin antibiotics on specific methanogenic activity of anaerobic biomass. Int. J. Environ. Health Eng. 1, 1–4. doi: 10.4103/2277-9183.102356
CrossRef Full Text
APUA. (2010). The Cost of Antibiotic Resistance to U.S Families and the Health Care System. A.f.t.P.U.o., ed. Available at: http://www.tufts.edu/med/apua/consumers/personal_home_5_1451036133.pdf
Carraschi, S. P., da Cruz, C., Machado Neto, J. G., Ignácio, N. F., Barbuio, R., and Machado, M. R. (2012). Histopathological biomarkers in pacu (Piaractus mesopotamicus) infected with Aeromonas hydrophila and treated with antibiotics. Ecotoxicol. Environ. Saf. 83, 115–120. doi: 10.1016/j.ecoenv.2012.06.016
Casewell, M., Friis, C., Marco, E., McMullin, P., and Phillips, I. (2003). The European ban on growth-promoting antibiotics and emerging consequences for human and animal health. J. Antimicrob. Chemother. 52, 159–161. doi: 10.1093/jac/dkg313
Center for Food Safety [CFS]. (2013). Animal Factories and Environmental Pollution. Available at: http://www.centerforfoodsafety.org/issues/307/animal-factories/animal-factories-and-environmental-pollution#
CIPARS. (2008). Canadian Integrated Program for Antimicrobial Resistance Surveillance Annual Report 2008. Available at: http://www.phac-aspc.gc.ca/cipars-picra/2008/index-eng.php
Cook, R. (1999). EU ban on four antibiotic growth promoters. Vet. Rec. 144, 158.
Cox, L. A. Jr. (2005). Potential human health benefits of antibiotics used in food animals: a case study of virginiamycin. Environ. Int. 31, 549–563. doi: 10.1016/j.envint.2004.10.012
Cromwell, C. L. (2002). Why and how antibiotics are used in swine production. Anim. Biotechnol. 13, 7–27. doi: 10.1081/ABIO-120005767
Cromwell, G. L. (1999). "Subtherapeutic use of antibiotics for swine: performance, reproductive efficiency and safety issues," in Proceeding of 40th George A. Young Swine Health and Management Conference, Lincoln, 69–87.
Cromwell, G. L., and Stahly, T. S. (1985). Efficacy of tiamulin as a growth promotant for growing swine. J. Anim. Sci. 60, 14–19.
Cromwell, G. L., Stahly, T. S., Jensen, A. H., Plumlee, M. P., Krider, J. L., and Russett, J. C. (1984a). Efficacy of thiopeptin as a growth promotant for growing barrows and gilts – a cooperative study. J. Anim. Sci. 59, 892–895.
Cromwell, G. L., Stahly, T. S., Speer, V. C., and O'Kelly, R. (1984b). Efficacy of nosiheptide as a growth promotant for growing-finishing swine – a cooperative study. J. Anim. Sci. 59, 1125–1128.
Cunha, T. J., Meadows, G. B., Edwards, H. M., Sewell, R. F., Pearson, A. M., and Glasscock, R. S. (1951). A comparison of aureomycin, streptomycin, penicillin and an aureomycin-B12 feed supplement for the pig. Arch. Biochem. 30, 269–271.
DANMAP. (2010). DANMAP – Use of Antimicrobial Agents and Occurrence of Antimicrobial Resistance in Bacteria from Food Animals, Food and Humans in Denmark. Available at: http://www.danmap.org
Dibner, J. J., and Richards, J. D. (2005). Antibiotic growth promoters in agriculture: history and mode of action. Poult. Sci. 84, 634–643. doi: 10.1093/ps/84.4.634
Dodds, W. K., Priscu, J. C., and Ellis, B. K. (1991). Seasonal uptake and regeneration of inorganic nitrogen and phosphorus in a large oligotrophic lake: size-fractionation and antibiotic treatment. J. Plankton Res. 13, 1339–1358. doi: 10.1093/plankt/13.6.1339
Domescik, E. J., and Martin, S. A. (1999). Effects of laidlomycin propionate and monensin on the in vitro mixed ruminal microorganism fermentation. J. Anim. Sci. 77, 2305–2312.
Doyle, M. P., and Erickson, M. C. (2006). Reducing the carriage of foodborne pathogens in livestock and poultry. Poult. Sci. 85, 960–973. doi: 10.1093/ps/85.6.960
Drouin, E. (1999). Helicobacter pylori: novel therapies. Can. J. Gastroenterol. 13, 581–583.
Elder, R. O., Keen, J. E., Wittum, T. E., Callaway, T. R., Edrington, T. S., Anderson, R. C., et al. (2002). "Intervention to reduce fecal shedding of enterohemorrhagic Escherichia coli O157:H7 in naturally infected cattle using neomycin sulfate," in American Society of Animal Science/American Dairy Science Association Joint Meeting, Quebec, 602.
Emborg, H. D., Ersboll, A. K., Heuer, O. E., and Wegener, H. C. (2002). "Effects of termination of antimicrobial growth promoter use for broiler health and productivity," in International Invitational Symposium; Beyond Anti Microbial Growth Promoters in Food Animal Production, November 6–7 2002, Foulum, 38–42.
EPC. (2005). Ban on Antibiotics as Growth Promoters in Animal Feed Enters into Effect, European Commission – IP/05/1687. Available at: http://europa.eu/rapid/press-release_IP-05-1687_en.htm
Falkow, S. (1970). Antibiotics in animal feeds. N. Engl. J. Med. 282, 693–694. doi: 10.1056/NEJM197003192821230
Food and Agriculture Organization (FAO). (2006). Livestock Impacts on the Environment, Food and Agriculture Organization of the United Nations, Agriculture and Consumer Protection Department. Available at: http://www.fao.org/ag/magazine/0612sp1.htm
Food and Agriculture Organization [FAO]. (2009). The State of Food and Agriculture: Livestock in the Balance. Available at: http://www.fao.org/docrep/012/i0680e/i0680e00.htm
Food and Agriculture Organization [FAO]. (2012). The State of Food Insecurity in the World 2012. Available at: http://www.fao.org/docrep/016/i3027e/i3027e00.htm
Food and Drug Administration (FDA). (1998). Freedom of information summary NADA 1412068, htm, h.w.f.g.c.f., ed.
Food and Drug Administration (FDA). (2002). Final decision of the Commissioner Docket No. 2000N21571 with drawal of approval of the new animal drug application for enrofloxacin in poultry [EB/OL]. Available at: [http://www.fda.gov/oc/antimicrobial/baytri.html]
Fellner, V., Sauer, F. D., and Kramer, J. K. (1997). Effect of nigericin, monensin, and tetronasin on biohydrogenation in continuous flow-through ruminal fermenters. J. Dairy Sci. 80, 921–928. doi: 10.3168/jds.S0022-0302(97)76015-6
Forman, C. R., and Burch, J. E. (1947). Use of sodium sulfonamides as single injection specific treatment in foot rot. J. Am. Vet. Med. Assoc. 111, 208–214.
Franklyn B. K. (2010). Poultry Culture, Sanitation and Hygiene (1915). Whitefish, MT: Kessinger Publishing.
Franti, C. E., Adler, H. E., and Julian, L. M. (1971). Antibiotic growth promotion: effects of bacitracin and oxytetracycline on intestines and selected lymphoid tissues of New Hampshire cockerels. Poult. Sci. 50, 94–99. doi: 10.3382/ps.0500094
Franti, C. E., Julian, L. M., Adler, H. E., and Wiggins, A. D. (1972). Antibiotic growth promotion: effects of zinc bacitracin and oxytetracycline on the digestive, circulatory, and excretory systems of New Hampshire cockerels. Poult. Sci. 51, 1137–1145. doi: 10.3382/ps.0511137
Gaggia, F., Mattarelli, P., and Biavati, B. (2010). Probiotics and prebiotics in animal feeding for safe food production. Int. J. Food Microbiol. 141(Suppl. 1), S15–S28. doi: 10.1016/j.ijfoodmicro.2010.02.031
Garcia-Migura, L., Liebana, E., Jensen, L. B., Barnes, S., and Pleydell, E. (2007). A longitudinal study to assess the persistence of vancomycin-resistant Enterococcus faecium (VREF) on an intensive broiler farm in the United Kingdom. FEMS Microbiol. Lett. 275, 319–325. doi: 10.1111/j.1574-6968.2007.00911.x
GIA. (2012). Animal Health Market to Hit $43 Billion in Five Years. Available at: http://westernfarmpress.com/management/animal-health-market-hit-43-billion-five-years
GRACE. (2013). The Issue: Antibiotics and the Food Animal Industry. Available at: http://www.sustainabletable.org/257/antibiotics, GRACE Communications Foundation.
Guan, H., Wittenberg, K. M., Ominski, K. H., and Krause, D. O. (2006). Efficacy of ionophores in cattle diets for mitigation of enteric methane. J. Anim. Sci. 84, 1896–1906. doi: 10.2527/jas.2005-652
Haenni, M., Saras, E., Chatre, P., Medaille, C., Bes, M., Madec, J. Y., et al. (2012). A USA300 variant and other human-related methicillin-resistant Staphylococcus aureus strains infecting cats and dogs in France. J. Antimicrob. Chemother. 67, 326–329. doi: 10.1093/jac/dkr499
Halling-Sorensen, B., Sengelov, G., Ingerslev, F., and Jensen, L. B. (2003). Reduced antimicrobial potencies of oxytetracycline, tylosin, sulfadiazin, streptomycin, ciprofloxacin, and olaquindox due to environmental processes. Arch. Environ. Contam. Toxicol. 44, 7–16. doi: 10.1007/s00244-002-1234-z
Hammerum, A. M., Heuer, O. E., Lester, C. H., Agerso, Y., Seyfarth, A. M., Emborg, H. D., et al. (2007). Comment on: withdrawal of growth-promoting antibiotics in Europe and its effects in relation to human health. Int. J. Antimicrob. Agents 30, 466–468. doi: 10.1016/j.ijantimicag.2007.07.012
Hardy, B. (1999). "A world without growth promoters," in First Annual Turtle Lake Science Conference (Nottingham: Nottingham University Press).
Harvey, R., Funk, J., Wittum, T. E., and Hoet, A. E. (2009). A metagenomic approach for determining prevalence of tetracycline resistance genes in the fecal flora of conventionally raised feedlot steers and feedlot steers raised without antimicrobials. Am. J. Vet. Res. 70, 198–202. doi: 10.2460/ajvr.70.2.198
He, S., Zhou, Z., Meng, K., Zhao, H., Yao, B., Ringo, E., et al. (2011). Effects of dietary antibiotic growth promoter and Saccharomyces cerevisiae fermentation product on production, intestinal bacterial community, and nonspecific immunity of hybrid tilapia (Oreochromis niloticus female × Oreochromis aureus male). J. Anim. Sci. 89, 84–92. doi: 10.2527/jas.2010-3032
Heikkila, A. M., Nousiainen, J. I., and Pyorala, S. (2012). Costs of clinical mastitis with special reference to premature culling. J. Dairy Sci. 95, 139–150. doi: 10.3168/jds.2011-4321
Hemaprasanth, K. P., Kar, B., Garnayak, S. K., Mohanty, J., Jena, J. K., and Sahoo, P. K. (2012). Efficacy of two avermectins, doramectin and ivermectin against Argulus siamensis infestation in Indian major carp, Labeo rohita. Vet. Parasitol. 190, 297–304. doi: 10.1016/j.vetpar.2012.05.010
Heuer, O. E., Pedersen, K., Andersen, J. S., and Madsen, M. (2001). Prevalence and antimicrobial susceptibility of thermophilic Campylobacter in organic and conventional broiler flocks. Lett. Appl. Microbiol. 33, 269–274. doi: 10.1046/j.1472-765X.2001.00994.x
Hewes, C. G. (1955). The influence on the growth and progeny of the guinea pig resulting from oral administration of aureomycin (chloretracycline) and penicillin. J. Nutr. 57, 353–360.
Hoflack, G., Maes, D., Mateusen, B., Verdonck, M., and de Kruif, A. (2001). Efficacy of tilmicosin phosphate (Pulmotil premix) in feed for the treatment of a clinical outbreak of Actinobacillus pleuropneumoniae infection in growing-finishing pigs. J. Vet. Med. 48, 655–664. doi: 10.1046/j.1439-0450.2001.00492.x
Hook, S. E., Wright, A. D., and McBride, B. W. (2010). Methanogens: methane producers of the rumen and mitigation strategies. Archaea 2010, 945785. doi: 10.1155/2010/945785
Hosking, B. C., Griffiths, T. M., Woodgate, R. G., Besier, R. B., Le Feuvre, A. S., Nilon, P., et al. (2009). Clinical field study to evaluate the efficacy and safety of the amino-acetonitrile derivative, monepantel, compared with registered anthelmintics against gastrointestinal nematodes of sheep in Australia. Aust. Vet. J. 87, 455–462. doi: 10.1111/j.1751-0813.2009.00511.x
Hower, S., Phillips, M. C., Brodsky, M., Dameron, A., Tamargo, M. A., Salazar, N. C., et al. (2013). Clonally related methicillin-resistant Staphylococcus aureus isolated from short-finned pilot whales (Globicephala macrorhynchus), human volunteers, and a Bayfront Cetacean Rehabilitation Facility. Microb. Ecol. 65, 1024–1038. doi: 10.1007/s00248-013-0178-3
Hurd, H. S., Brudvig, J., Dickson, J., Mirceta, J., Polovinski, M., Matthews, N., et al. (2008). Swine health impact on carcass contamination and human foodborne risk. Public Health Rep. 123, 343–351.
Hurd, H. S., Gailey, J. K., McKean, J. D., and Griffith, R. W. (2005). Variable abattoir conditions affect Salmonella enterica prevalence and meat quality in swine and pork. Foodborne Pathog. Dis. 2, 77–81. doi: 10.1089/fpd.2005.2.77
Hurd, H. S., Yaeger, M. J., Brudvig, J. M., Taylor, D. D., and Wang, B. (2012). Lesion severity at processing as a predictor of Salmonella contamination of swine carcasses. Am. J. Vet. Res. 73, 91–97. doi: 10.2460/ajvr.73.1.91
IFAH-EuroP. (2005). IFAH – Europe Annual Report 2005. Available at: http://www.ifaheuroPe.org/Publieations/IFAH_EuroPe_AR_2005.Pdf
Jensen, G. B., Hansen, B. M., Eilenberg, J., and Mahillon, J. (2003). The hidden lifestyles of Bacillus cereus and relatives. Environ. Microbiol. 5, 631–640. doi: 10.1046/j.1462-2920.2003.00461.x
JETACAR. (1999). The Use of Antibiotics in Food Producing Animals. Report of the Joint Expert Advisory Committee on Antibiotic Resistance. Canberra: Commonwealth of Australia, Resistance, 107–116.
Jones, C. M. (2010). The Effects of Selected Antibiotics on Nitrogen Uptake by Spirodela Punctata. M.S. thesis, Humboldt State University, Arcata.
Jones, R. L. (2000). Clostridial enterocolitis. Vet. Clin. N. Am. 16, 471–485.
Jukes, T. H. (1970). Antibiotics in animal feeds. N. Engl. J. Med. 282, 49–50. doi: 10.1056/NEJM197001012820116
JVARM. (2008). A Report on the Japanese Veterinary Antimicrobial Resistance Monitoring System 2002-2007. Tokyo: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries.
Kantas, D., Vassilopoulos, V., Kyriakis, S. C., and Saoulidis, K. (1998). A dose titration study on the effect of virginiamycin on gilt/sow and piglet performance. Zentralb. Veterinarmed. 45, 525–533. doi: 10.1111/j.1439-0442.1998.tb00856.x
Knarreborg, A., Lauridsen, C., Engberg, R. M., and Jensen, S. K. (2004). Dietary antibiotic growth promoters enhance the bioavailability of alpha-tocopheryl acetate in broilers by altering lipid absorption. J. Nutr. 134, 1487–1492.
Kobayashi, Y. (2010). Abatement of methane production from ruminants: trends in the manipulation of rumen fermentation Asian-Aust. J. Anim. Sci. 23, 410–416.
Krausse, R., and Schubert, S. (2010). In-vitro activities of tetracyclines, macrolides, fluoroquinolones and clindamycin against Mycoplasma hominis and Ureaplasma spp. isolated in Germany over 20 years. Clin. Microbiol. Infect. 16, 1649–1655.
Krehbiel, C. (2013). The role of new technologies in global food security: Improving animal production efficiency and minimizing impacts. Anim. Front. 3, 4–7. doi: 10.2527/af.2013-0017
Lallai, A., Mura, G., and Onnis, N. (2002). The effects of certain antibiotics on biogas production in the anaerobic digestion of pig waste slurry. Bioresour. Technol. 82, 205–208. doi: 10.1016/S0960-8524(01)00162-6
Larsen, J., Anderson, N., and Preshaw, A. (2009). Long-acting moxidectin for the control of trichostrongylid infections of sheep in south-eastern Australia. Aust. Vet. J. 87, 130–137. doi: 10.1111/j.1751-0813.2009.00395.x
Lindemann, M. D., and Kornegay, E. T. (1989). Folic acid supplementation to diets of gestating-lactating swine over multiple parities. J. Anim. Sci. 67, 459–464.
Lindemann, M. D., Kornegay, E. T., Stahly, T. S., Cromwell, G. L., Easter, R. A., Kerr, B. J., et al. (1985). The efficacy of salinomycin as a growth promotant for swine from 9 to 97 kg. J. Anim. Sci. 61, 782–788.
Loftin, K. A., Henny, C., Adams, C. D., Surampali, R., and Mormile, M. R. (2005). Inhibition of microbial metabolism in anaerobic lagoons by selected sulfonamides, tetracyclines, lincomycin, and tylosin tartrate. Environ. Toxicol. Chem. 24, 782–788. doi: 10.1897/04-093R.1
Loncaric, I., Kubber-Heiss, A., Posautz, A., Stalder, G. L., Hoffmann, D., Rosengarten, R., et al. (2013). Characterization of methicillin-resistant Staphylococcus spp. carrying the mecC gene, isolated from wildlife. J. Antimicrob. Chemother. 68, 2222–2225. doi: 10.1093/jac/dkt186
Lovland, A., and Kaldhusdal, M. (2001). Severely impaired production performance in broiler flocks with high incidence of Clostridium perfringens-associated hepatitis. Avian Pathol. 30, 73–81. doi: 10.1080/03079450020023230
Matthews, K. (2001). Antimicrobial drug use and veterinary costs in U.S. livestock production, Agriculture Information Bulletin No. (AIB-766), 761–768.
Manson, E. R. (1968). Antibiotics in animal feeds. Aust. Vet. J. 44, 169–173. doi: 10.1111/j.1751-0813.1968.tb09069.x
Marshall, B. M., and Levy, S. B. (2011). Food animals and antimicrobials: impacts on human health. Clin. Microbiol. Rev. 24, 718–733. doi: 10.1128/CMR.00002-11
McKellar, Q. A., Midgley, D. M., Galbraith, E. A., Scott, E. W., and Bradley, A. (1992). Clinical and pharmacological properties of ivermectin in rabbits and guinea pigs. Vet. Rec. 130, 71–73. doi: 10.1136/vr.130.4.71
Mead, P. S., Slutsker, L., Dietz, V., McCaig, L. F., Bresee, J. S., Shapiro, C., et al. (1999a). Food-related illness and death in the United States. Emerg. Infect. Dis. 5, 607–625. doi: 10.3201/eid0505.990502
Mead, P. S., Slutsker, L., Griffin, P. M., and Tauxe, R. V. (1999b). Food-related illness and death in the United States reply to Dr. Hedberg. Emerg. Infect. Dis. 5, 841–842. doi: 10.3201/eid0506.990625
Mellata, M. (2013). Human and avian extraintestinal pathogenic Escherichia coli: infections, zoonotic risks, and antibiotic resistance trends. Foodborne Pathog. Dis. 10, 916–932. doi: 10.1089/fpd.2013.1533
Midtvedt, T. (1986). Effects of antimicrobial agents upon the functional part of the intestinal flora. Scand. J. Infect. Dis. 49, 85–88.
Moore, P. R., and Evenson, A. (1946). Use of sulfasuxidine, streptothricin, and streptomycin in nutritional studies with the chick. J. Biol. Chem. 165, 437–441.
Nagaraja, T. G., and Taylor, M. B. (1987). Susceptibility and resistance of ruminal bacteria to antimicrobial feed additives. Appl. Environ. Microbiol. 53, 1620–1625.
NARMS. (2010). Retail Meat Report – National Antimicrobial Resistance Monitoring System. Available at: http://www.fda.gov/AnimalVeterinary/SafetyHealth/AntimicrobialResistance/NationalAntimicrobialResistanceMonitoringSystem/default.htm
Nelson, J. M., Chiller, T. M., Powers, J. H., and Angulo, F. J. (2007). Fluoroquinolone-resistant Campylobacter species and the withdrawal of fluoroquinolones from use in poultry: a public health success story. Clin. Infect. Dis. 44, 977–980. doi: 10.1086/512369
Niewold, T. A. (2007). The nonantibiotic anti-inflammatory effect of antimicrobial growth promoters, the real mode of action? A hypothesis. Poult. Sci. 86, 605–609. doi: 10.1093/ps/86.4.605
Norin, K. E. (1997). Influence of antibiotics on some intestinal microflora associated characteristics. Anaerobe 3, 145–148. doi: 10.1006/anae.1997.0091
Nunes, K. (2008). Hogs raised without antibiotics carry more pathogens. Food Business News, 12th June.
Odongo, N. E., Bagg, R., Vessie, G., Dick, P., Or-Rashid, M. M., Hook, S. E., et al. (2007). Long-term effects of feeding monensin on methane production in lactating dairy cows. J. Dairy Sci. 90, 1781–1788. doi: 10.3168/jds.2006-708
Partanen, K., Siljander-Rasi, H., Pentikainen, J., Pelkonen, S., and Fossi, M. (2007). Effects of weaning age and formic acid-based feed additives on pigs from weaning to slaughter. Arch. Anim. Nutr. 61, 336–356. doi: 10.1080/17450390701556866
Phillips, G. (1999). Microbiological aspects of clinical waste. J. Hosp. Infect. 41, 1–6. doi: 10.1016/S0195-6701(99)90029-4
Phillips, I. (2007). Withdrawal of growth-promoting antibiotics in Europe and its effects in relation to human health. Int. J. Antimicrob. Agents 30, 101–107. doi: 10.1016/j.ijantimicag.2007.02.018
Phillips, I., Casewell, M., Cox, T., De Groot, B., Friis, C., Jones, R., et al. (2004a). Antibiotic use in animals. J. Antimicrob. Chemother. 53, 885. doi: 10.1093/jac/dkh149
Phillips, I., Casewell, M., Cox, T., De Groot, B., Friis, C., Jones, R., et al. (2004b). Does the use of antibiotics in food animals pose a risk to human health? A critical review of published data. J. Antimicrob. Chemother. 53, 28–52. doi: 10.1093/jac/dkg483
Poduval, R. D., Kamath, R. P., Corpuz, M., Norkus, E. P., and Pitchumoni, C. S. (2000). Clostridium difficile and vancomycin-resistant enterococcus: the new nosocomial alliance. Am. J. Gastroenterol. 95, 3513–3515. doi: 10.1111/j.1572-0241.2000.03291.x
Reports, Q. (2012). China Veterinary Drug Industry 2012 Market Research Report. Available at: http://www.qyresearchreports.com/report/china-veterinary-drug-industry-2012-market-research-report.htm
Rodehutscord, M., Abel, H. J., Friedt, W., Wenk, C., Flachowsky, G., Ahlgrimm, H. J., et al. (2002). Consequences of the ban of by-products from terrestrial animals in livestock feeding in Germany and the European Union: alternatives, nutrient and energy cycles, plant production, and economic aspects. Archiv fur Tierernahrung 56, 67–91. doi: 10.1080/00039420214180
Rose, N., Beaudeau, F., Drouin, P., Toux, J. Y., Rose, V., and Colin, P. (1999). Risk factors for Salmonella enterica subsp. enterica contamination in French broiler-chicken flocks at the end of the rearing period. Prev. Vet. Med. 39, 265–277. doi: 10.1016/S0167-5877(99)00002-1
Rumpler, W. V., Johnson, D. E., and Bates, D. B. (1986). The effect of high dietary cation concentration on methanogenesis by steers fed diets with and without ionophores. J. Anim. Sci. 62, 1737–1741.
Russell, J. B., and Jeraci, J. L. (1984). Effect of carbon monoxide on fermentation of fiber, starch, and amino acids by mixed rumen microorganisms in vitro. Appl. Environ. Microbiol. 48, 211–217.
Russell, S. M. (2003). The effect of air sacculitis on bird weights, uniformity, fecal contamination, processing errors, and populations of Campylobacter spp. and Escherichia coli. Poult. Sci. 82, 1326–1331. doi: 10.1093/ps/82.8.1326
Sarmah, A. K., Meyer, M. T., and Boxall, A. B. (2006). A global perspective on the use, sales, exposure pathways, occurrence, fate and effects of veterinary antibiotics (VAs) in the environment. Chemosphere 65, 725–759. doi: 10.1016/j.chemosphere.2006.03.026
Scarfe, A. D., Lee, C.-S., and O'Bryen, P. J. (2011). Aquaculture Biosecurity: Prevention, Control, and Eradication of Aquatic Animal Disease. Oxford: Blackwell Publishers.
Schelling, G. T. (1984). Monensin mode of action in the rumen. J. Anim. Sci. 58, 1518–1527.
Shi, J. C., Liao, X. D., Wu, Y. B., and Liang, J. B. (2011). Effect of antibiotics on methane arising from anaerobic digestion of pig manure. Anim. Feed Sci. Technol. 166–167, 457–463. doi: 10.1016/j.anifeedsci.2011.04.033
Soma, J. A., and Speer, V. C. (1975). Effects of pregnant mare serum and chlortetracycline on the reproductive efficiency of sows. J. Anim. Sci. 41, 100–105.
Sun, J. P., Zheng, P., and Hu, B. L. (2009). [Combined effect of antibiotics on anaerobic digestion of piggery wastewater]. Huan jing ke xue = Huanjing kexue / [bian ji, Zhongguo ke xue yuan huan jing ke xue wei yuan hui. Huan jing ke xue 30, 2619–2624.
Taylor, J. H., and Gordon, W. S. (1955). Growth-promoting activity for pigs of inactivated penicillin. Nature 176, 312–313. doi: 10.1038/176312a0
Tice, A. (2001). Outpatient parenteral antimicrobial therapy (OPAT): a global perspective. Introduction. Chemotherapy 47(Suppl. 1), 1–4. doi: 10.1159/000048562
Turnidge, J. (2004). Antibiotic use in animals – prejudices, perceptions and realities. J. Antimicrob. Chemother. 53, 26–27. doi: 10.1093/jac/dkg493
USFDA. (2009). Summary Report on Antimicrobials Sold or Distributed for Use in Food-Producing Animals. Department of Health and Human Services. Available at: http://www.fda.gov/downloads/ForIndustry/UserFees/AnimalDrugUserFeeActADUFA/UCM231851.pdf
Van Lunen, T. A. (2003). Growth performance of pigs fed diets with and without tylosin phosphate supplementation and reared in a biosecure all-in all-out housing system. Can. Vet. J. 44, 571–576.
Van Nevel, C., and Demeyer, D. I. (1995). Lipolysis and biohydrogenation of soybean oil in the rumen in vitro: inhibition by antimicrobials. J. Dairy Sci. 78, 2797–2806. doi: 10.3168/jds.S0022-0302(95)76910-7
Van Nevel, C. J., and Demeyer, D. I. (1977). Effect of monensin on rumen metabolism in vitro. Appl. Environ. Microbiol. 34, 251–257.
Varga, C., Rajic, A., McFall, M. E., Reid-Smith, R. J., Deckert, A. E., Checkley, S. L., et al. (2009). Associations between reported on-farm antimicrobial use practices and observed antimicrobial resistance in generic fecal Escherichia coli isolated from Alberta finishing swine farms. Prev. Vet. Med. 88, 185–192. doi: 10.1016/j.prevetmed.2008.10.002
Verkade, E., and Kluytmans, J. (2013). Livestock-associated Staphylococcus aureus CC398: animal reservoirs and human infections. Infect. Genet. Evol. 21, 523–530. doi: 10.1016/j.meegid.2013.02.013
Vieira, A. R., Collignon, P., Aarestrup, F. M., McEwen, S. A., Hendriksen, R. S., Hald, T., et al. (2011). Association between antimicrobial resistance in Escherichia coli isolates from food animals and blood stream isolates from humans in Europe: an ecological study. Foodborne Pathog. Dis. 8, 1295–1301. doi: 10.1089/fpd.2011.0950
Vogel, G., Nicolet, J., Martig, J., Tschudi, P., and Meylan, M. (2001). Pneumonia in calves: characterization of the bacterial spectrum and the resistance patterns to antimicrobial drugs. Schweiz. Arch. Tierheilkd. 143, 341–350.
Wahlstrom, R. C., Terrill, S. W., and Johnson, B. C. (1950). Effect of antibacterial agents on growth of baby pigs fed a "synthetic" diet. Proc. Soc. Exp. Biol. Med. 75, 710–711. doi: 10.3181/00379727-75-18314
Weber, T. E., Schinckel, A. P., Houseknecht, K. L., and Richert, B. T. (2001). Evaluation of conjugated linoleic acid and dietary antibiotics as growth promotants in weanling pigs. J. Anim. Sci. 79, 2542–2549.
Werner, G., Coque, T. M., Hammerum, A. M., Hope, R., Hryniewicz, W., Johnson, A., et al. (2008). Emergence and spread of vancomycin resistance among enterococci in Europe. Euro Surveill. 13, pii: 19046.
Wierup, M. (2001). The Swedish experience of the 1986 year ban of antimicrobial growth promoters, with special reference to animal health, disease prevention, productivity, and usage of antimicrobials. Microb. Drug Resist. 7, 183–190. doi: 10.1089/10766290152045066
Yano, Y., Yokoyama, M., Satomi, M., Oikawa, H., and Chen, S. S. (2004). Occurrence of Vibrio vulnificus in fish and shellfish available from markets in China. J. Food Prot. 67, 1617–1623.
Zhang, J. J., Wang, L. X., Ruan, W. K., and An, J. (2013). Investigation into the prevalence of coccidiosis and maduramycin drug resistance in chickens in China. Vet. Parasitol. 191, 29–34. doi: 10.1016/j.vetpar.2012.07.027
Zhou, Z., He, S., Liu, Y., Cao, Y., Meng, K., Yao, B., et al. (2011). Gut microbial status induced by antibiotic growth promoter alters the prebiotic effects of dietary DVAQUA(R) on Aeromonas hydrophila-infected tilapia: production, intestinal bacterial community and non-specific immunity. Vet. Microbiol. 149, 399–405. doi: 10.1016/j.vetmic.2010.11.022
Keywords: antimicrobial drug, benefit, risk, animal food production, public health
Citation: Hao H, Cheng G, Iqbal Z, Ai X, Hussain HI, Huang L, Dai M, Wang Y, Liu Z and Yuan Z (2014) Benefits and risks of antimicrobial use in food-producing animals. Front. Microbiol. 5:288. doi: 10.3389/fmicb.2014.00288
Received: 22 February 2014; Accepted: 24 May 2014;
Published online: 12 June 2014.
Robert Paul Hunter, Lilly, USA
Jer-Horng Wu, National Cheng Kung University, Taiwan
Copyright © 2014 Hao, Cheng, Iqbal, Ai, Hussain, Huang, Dai, Wang, Liu and Yuan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Zonghui Yuan, MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, China e-mail: yuan5802@mail.hzau.edu.cn


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19.96 lakh ghost beneficiaries identified at Assam Anganwadi centres: RTI reply
Sep 22, 2019, 11:25 am IST
India, All India
The Centre has asked all states to conduct headcount of all beneficiaries of Anganwadi centres.
The ministry further said the beneficiaries under the Anganwadi Services Scheme are identified on the basis of Aadhaar which is used as identity document for delivery of services or benefits in a bid to bring transparency and efficiency. (Photo: Representational)
New Delhi: As many as 19.96 lakh ghost beneficiaries have been identified at Anganwadi centres in Assam through a daily headcount, according to an RTI query.
In Assam, there are a total of 62,153 Anganwadi centres in which 36,24,973 beneficiaries, including 5,94,296 pregnant women and lactating mothers, are enrolled.
The Centre has asked all states to conduct headcount of all beneficiaries of Anganwadi centres, a type of rural child care centre set up by the government to combat undernutrition and stunting among children below the age of six years and also meet nutritional needs of pregnant and lactating women.
In reply to an RTI query, the Women and Child Development Ministry said a total of 19.96 lakh ghost beneficiaries (both children and pregnant women and lactating mothers) have been eliminated from the Anganwadi services in Assam while report from other state governments are still awaited.
The ministry further said the beneficiaries under the Anganwadi Services Scheme are identified on the basis of Aadhaar which is used as identity document for delivery of services or benefits in a bid to bring transparency and efficiency.
"The beneficiaries not possessing Aadhaar card are assisted by the field functionaries to obtain the Aadhaar card. Till such time they get the Aaadhar card, they are provided the Anganwadi services on the basis of alternative identification document," according to the RTI reply.
Officials said the identification of fake beneficiaries is an ongoing exercise and a report in this regard has been received just from Assam yet.
There are around 14 lakh Anganwadis across the country and 10 crore beneficiaries, which include children under the age of six and pregnant women and lactating mothers. According to the revised cost norms for supplementary nutrition, children from six to 72 months get Rs 8 per day while severely malnourished children (6-72 months) get Rs 12 per day and pregnant women and nursing mothers get Rs 9.50 per day.
The identification of fake beneficiaries is also being done through smartphones having Integrated Child Development Services-Common Application Software (ICDS-CAS) app, officials said.
These smartphones have been distributed to over 1.2 lakh Anganwadi centres. The nutrition monitoring app has been developed under the Poshan Abhiyan, also called the National Nutrition Mission, launched by the Centre in 2018 with an aim to bring down stunting in children in the age group of 0-6 years from 38.4 per cent to 25 per cent by 2022.
women and child development ministry
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redpajama_set_name: RedPajamaCommonCrawl


A series of storms that hit Scotland's Shetland Islands over the holidays revealed what archaeologists believe could be 2,000-year-old human remains.
Local archaeologist Chris Dyer said the ancient skeleton looked as if it were contemporary with the remains of Iron Age structures revealed nearby. Researchers then identified evidence of one or possibly two more burials at the site, but another storm caused a further chunk of the cliff to crumble, covering up the discovery.


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Dr. Pavan Duggal (Conference Co-Chair), Advocate, Supreme Court of India
(Conference Co-Chair), Advocate, Supreme Court of India
Rafael Narezzi
CyberEdTalks Brazil
Srinivas Poosarla
Infosys Technologies
Cloud Security , Security Operations
Chinese Group Targeting Vulnerable Cloud Providers, Apps
Cryptomining Campaign Targets Public Cloud Environments, Increases Security Risks Prajeet Nair (@prajeetspeaks) • January 21, 2023
Credit Eligible
Cybersecurity researchers say a Chinese for-profit threat group tracked as 8220 Gang is targeting cloud providers and poorly secured applications with a custom-built crypto miner and IRC bot.
See Also: Rapid Digitization and Risk: A Roundtable Preview
The group employs a variety of tactics and techniques to hide its activities and evade detection, including the use of a blocklist to avoid tripping over honeypots. "Yet, the group is not perfect and was caught attempting to infect one of Radware's Redis honeypots at the beginning of this year," according to cybersecurity firm Radware.
The threat groups specializes in cryptomining campaigns that target public cloud environments for several reasons, including because they offer potential targets with sufficient or elastic computing resources, researchers say.
"Many organizations have limited visibility, making it more difficult for security and network operations to detect and respond to security threats, and the public cloud providers also offer limited security controls, making it easier for threat actors to find and exploit vulnerabilities," researchers say.
Attack Methods
The attackers used a custom crypto miner, "PwnRig," that slows down systems using CPU and GPU resources. This also causes devices to become unresponsive, which causes elastic compute nodes to expand their resources and ultimately results in "a huge, unexpected invoice for the victim at the end of the billing cycle."
Daniel Smith, head of research for Radware's Threat Intelligence division, tells Information Security Media Group that the main concern with cryptomining malware is that it can significantly affect a system's performance and can also expose systems to additional security risks.
"Once infected, threat actors, can use the same access to install other types of malware, such as keyloggers or remote access tools, which can subsequently be leveraged to steal sensitive information, gain unauthorized access to sensitive data or deploy ransomware and wipers," Smith says.
The threat group was also found infecting devices using the Tsunami IRC bot - aka kaiten, which it uses as a backdoor. Tsunami is one of the earliest IoT botnets. It dates back to 2001 and uses the IRC protocol for command and control.
An IRC bot is a set of scripts or an independent program that connects to internet relay chat as a client. The bot allows the threat actors to remotely control systems and launch distributed denial-of-service attacks.
"The Tsunami IRC bot supports four different types of denial-of-service attacks, including SYN and UDP floods. DDoS attacks from the Tsunami bot can significantly impact targeted websites or networks by degrading services or making them unavailable for legitimate users, resulting in financial losses for the victim," according to Radware researchers.
The source IP address for the attack was a compromised Apache server hosted on a major cloud provider, researchers say. This IP address sent a series of scripted commands to Radware's Redis honeypot using /api/login and port 8443.
"These commands were cron jobs intended to download, install and execute a shell script named xms?redis, a Python script named d.py, a crypto miner called PwnRig, and the Tsunami IRC bot on the system where Redis is running.
Once these payloads are downloaded and executed, the shell script runs a series of commands designed to modify the system's configurations and settings. The commands from xms?redis create two new directories and give anyone permission to read, write and execute files in these directories.
"The commands also disable SELinux, set a limit on the number of processes a user can have running at one time, disable the firewall management tool, ufw, and removes attributes from the /etc/ls.so.preload file on targeted systems," researchers say.
The commands also executes a function to remove the security tools from several cloud providers, and it checks if outbound communications are "blocked by security components on targeted devices and deletes log files that may contain suspicious activity."
The 8220 Gang uses multiple scan functions to discover new potential targets, brute-force ssh services and collect ssh keys.
"The threat to cloud environments and insecure applications continues to pose risks to organizations around the world, especially those that use weak credentials or do not patch vulnerabilities immediately. Because of poor security hygiene, low-skilled groups like the 8220 Gang are able to cause a significant impact on targeted systems," Smith says.
Radware researchers recommend that organizations adopt a comprehensive security strategy that includes security controls, monitoring, and incident response capabilities to protect their cloud environments and applications from cryptomining campaigns.
It also advises organizations to ensure their security controls provide visibility into their hybrid and multi-cloud environment to detect and respond to new security threats.
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From heightened risks to increased regulations, senior leaders at all levels are pressured to improve their organizations' risk management capabilities. But no one is showing them how - until now.
Learn the fundamentals of developing a risk management program from the man who wrote the book on the topic: Ron Ross, computer scientist for the National Institute of Standards and Technology. In an exclusive presentation, Ross, lead author of NIST Special Publication 800-37 - the bible of risk assessment and management - will share his unique insights on how to:
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text:
Daniele Ciullini – Domestic Exile (Collected Works 82-86)
by Juno Plus on 27.03.2015 at 11:07am
A few years ago The Guardian decided to run a series on Italian popular music, part of which involved an interview with Alessio Natalizia of Walls. Here Natalizia was asked about the differences between making music in Italy and in the UK. "What does Italian music sound like, anyway?" Natalizia retorted, adding, "we've never been able to take Italian pop music around the world in the same way we have with food." After a few Walls and Not Waving records, and having curated the Mutazione compilation issued through Strut Records, here comes Natalizia taking Italian music around the world in the form of Daniele Ciullini with Domestic Exile (Collected Works 82-86).
Daniele Ciullini
Domestic Exile (Collected Works 82-86)
Ecsatatic Recordings
Released on Natalizia's own Ecstatic Recordings, this retrospective includes Ciullini's magnum opus – the 1983 30-minute self-released tape Domestic Exile – and another eight tracks taken from tapes here and there from the early 1980s, remastered and cleaned up for the contemporary ear. A member of the many-headed TRAX project who combined mail-art, design, music and indeed mail-music, Cuillini was deeply connected to the work of mailartist Vittore Baroni, the Florence punk scene and the team behind the dream of public broadcasting that was RAI's programme Fonosfera. Ciullini collaborated on a number of wildly ingenious collaborations, some of which are also repressed here.
Writing about the album, Ciullini once explained how it was made in a room where his father used to design water turbines, which Ciullini later fitted out with portraits of Lenin, Marx and Che Guevara. He then emptied it out completely, installed his rudimentary 'toys' and made Domestic Exile. And in a sense, that's how the record sounds. Domestic Exile is a cerebral and solitary distillation of a kind of spiky environmental music. Ambient for a small, white room, a room where you can feel the grain of the walls, the grain of time, and your own heartbeat.
The record's first track, "Violet" – rusty but spacey at the same time – is an introduction to the echoed and tinny sounds that reappear time and again in Domestic Exile, overlaid in the thumpy, dense punches of a TR-606 drum machine. The most forceful of these moments – "Marbles in the Garden" and "The Shadow Whisper" – could be club tracks suited to an obsessive, minimal dance. The most delicate, romantic turns, such as "Decadence", move into a kind of industrial-indebted auratic and even cosmic-tinged atmosphere. In fact, although the concept of the album is a kind of bleak isolation, the sonic concept and the equipment has something stellar about it, something like hearing stars colliding.
Variating on a series of themes – minimal, quasi-geometric patterns in which a drum machine, a modular synthesizer and a heavily reverbed guitar step in and out with each other – Ciullini's sound is a sort of brutal ambient, artisanal-punk, but almost digital in sensibility. "Naked and White" is a ghostly fugue, with crystalline sounds variating ever so slightly on a simple melody, underpinned the whole time by a thick, dark bassline. "Lisptick on the Glasses" distorts something almost like a police-movie sound into a mercurial, shifting, complex.
Highlights from the precious scattered material lovingly preserved in the second part of the LP include "Silence", a rhythmically easier but extremely atmospheric piece and the sophisticated "Notte Rossa" tracks, part of a wider TRAX project which invited artists sending each other unfinished tapes and overlay tracks one upon the other. The second part is precious because we hear the musician work with other, sometimes more sophisticated instruments – the meditative, fluttery electronics of "Networking" are a testament not only to an evident flair but also to a kind of research, which, thankfully, Ciullini has recently begun to undertake again.
On the almost shoegazey "Soft Marble", before the opaque drones of "Bloody Machine" which close the LP, Ciullini depressedly croones 'nobody will open tomorrow's door'. Not true. Here we are unearthing some of the most glorious segments of home-made Italian music and, indeed, 'taking it around the world'. If we're going to continue with the food comparison, this is some kind of tartare with truffle and puntarelle, and why not, made by some punk nun who lives alone on a hill with a recipe that hasn't changed since the 1600s. It's the bitter, metallic, earthy end of Italian music, made on the blade of a knife. It's a good time for the Italian archive. Our palates, I think, are maturing.
Flora Pitrolo
1. Violet
2. Marbles In The Garden
3. Trance
4. Lipstick On The Glasses
5. Decadence
6. Naked And White
7. Flowers In The Water
8. The Shadow Whisper
9. Deep Water
10. Silence
11. Silence 3
13. Notte Rossa A
14. Notte Rossa B
15. Soft Marble
16. Bloody Machine


This is useful for concentration in spiritual practice and awakening of spiritual emotion. It restores the level of comfort and calmness in the Atmosphere by rejuvenating individual's spirituality. The purpose of burning Agarbatti is to purify the bad air. The holy smoke prevents unwanted bad energy and bad spirit from entering home.
§ Guggal is anti-ageing in nature.
§ Benefit of Guggal is that it eradicates negativity and brings in prosperity.
Guggal is considered as a remedy for vastu defects and also helps to cleanse the aura.uponburning,guggal gives dense and aromatic smoke, which is then taken around in the house and held in all the rooms for a couple of minutes.


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Peridot Acquisition Corp.
Peridot Acquisition Corp. II
Tomas Ackerman
Tomas Ackerman has been our Chairman since March 2021. Mr. Ackerman is a Co-Founder and a Partner of Carnelian, where he oversees Carnelian's efforts in sourcing investments, transaction negotiation and execution, monitoring of portfolio companies and firm management and strategy. Prior to co-founding Carnelian, Mr. Ackerman was an energy investor at Natural Gas Partners from 2002 to 2015, most recently serving as a Managing Director and the head of the Houston office. Mr. Ackerman received a B.B.A. from the Business Honors Program at The University of Texas at Austin and an M.B.A. from Harvard Business School.
Copyright © 2023 Peridot SPAC • All Rights Reserved


London is plagued by street gangs. By the year 2016 there were an estimated 3,600 gangsters. According to government figures, these form some 225 gangs. Of these, 58 gangs are regularly active and are thought by police to be responsible for two-thirds of gang-related offences, including assault, theft, murder and, most of all, drugs. Ethnically, gangsters are mainly white, Asian, black and Eastern European. In the absence of official data, on-the-ground reports suggest that the majority of gangsters dealing in drugs, where the most violent crimes occur, are young black males, particularly Jamaican.
This is not only a symptom of how successive British governments have failed young ethnic minorities, it reflects the tragic legacy of colonialism.
Jamaica's pre-Independence gangs, like The Yardies, emerged from the poverty of the 1950s. Caribbeans experienced similar hardships when they and their parents moved to the UK after the Second World War. According to the British National Archives, between 1948 and 1970, almost half a million people from the West Indies (including the Caribbean) came to Britain, many of them on government initiatives, "to run the transport system, postal service and hospitals. Other West Indians were returning soldiers who had fought for Britain during the Second World War." Most of the immigrants settled in London. One academic paper notes that "Britain's experience of West Indian immigration" was "traumatic … Both first and second generations in the U.K. have experienced open hostility" from media, politicians and the public. Inner city violence, including white gangs vs. black gangs, affected Liverpool in the north, Handsworth in the Midlands and, in London, Brixton, Notting Hill and Tottenham.
Within a generation, poverty and discrimination in London had given rise to the gangland culture, with children growing up poor and oppressed and turning to crime for prestige and money. In 1992, the British police launched Operation Lucy to crush the gangs. Not having learned their lessons, successive governments, including the Tories under Margaret Thatcher and John Major, as well as New Labour under Tony Blair and Gordon Brown, failed to address the grievances of young ethnic minority Britons–the children and grandchildren of the migrant generations–and help them integrate into society. As in previous decades, some youngsters of this neglected generation have turned to gangs for profit and protection.
Britain has been condemned by the United Nations for its structural racism. Just 14% percent of British people are from ethnic minorities. Yet 26% of British prisoners are non-whites, with black offenders 53% more likely to be sent to prison than whites. By 2011, black people were, in some areas of the UK, 28 times more likely than white people to be stopped and searched by police. The children of black Caribbeans are three times more likely than white children to be permanently expelled from school.
According to government figures, by 2016 the rate of employment for white Britons was 74.7%. For black Britons, however, it was 59.3%. While the rate of overall poverty for white Britons (according to the government's questionable measure of poverty) was 17.2%, for black Britons it was 39.9%. The percentage of white people likely to get a good degree at college is 76.3% compared to 60.3% for ethnic minorities in general. Although just 7.3% of the British population supposedly experiences "persistent poverty" (again, by the government's dubious measure), for black and Asian households, the percentage rises to 20. Poverty, unemployment, imprisonment and general discrimination put massive strains on ethnic minority families, hence many absent fathers. Twenty-two percent of white parents are single, but 51% of black parents are single. There are few means for ethnic minority women to make a decent living. By 2011, unemployment for ethnic women was 14% compared to 6.8% for white women.
British governments used Caribbeans and their African ancestors as slaves and then as cheap labour to rebuild post-war Britain. Having been exploited, they and their offspring have been left to face poverty and discrimination.
Another indication of how badly the system has failed these young, mainly ethnic minority males is the extent to which many believe in casting magic spells (Obeah) for self-protection. Here, the link between modern gangland violence and colonialism is apparent. According to a four-part investigation into London gangs by the International Business Times (IBT), Obeah originated in the west African Ashanti. Centuries ago, the practitioners believed that their spells could protect them. The magicians were respected and feared. The Spanish and later English slave traders brought them and their tradition to the Caribbean and thus to modern London.
The IBT investigation concludes that many of today's black London gangsters believe that Obeah will protect them from the police and bullets of rival gangs. It is significant that in the past the practitioners of Obeah "played a key role in slave rebellions and would create powders that supposedly possessed magical properties that would protect users from the white man's weapons." Today, the white man's weapons are job discrimination, racism in policing, an economic system that favours wealthy and middle-class people, and the refusal of successive governments to get to the root of what drives young males to join gangs. Like so many things, London's gang crisis is in no small part one of the tragic legacies of colonialism.


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I had save this recipe cause I knew it would be good, and it proved to be the best one I've ever made. My ratios of spices and peppers were a little altered, and I had a can of Muir Glen fire roasted, crushed tomatoes which added a little more depth perhaps, but it's a big winner. I filed this in "Make Again� for sure! Thank you – love your emails.


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EQCO2 (OTCMKTS:CLNO) Announces Changes to its Board of Directors and Principal Officers
Posted by admin On February 01, 2016 0 Comment
Las Vegas, NV, Feb. 1, 2016 – EQCO2, Inc. (OTCMKTS:CLNO) today announced the resignation of Harold Gewerter as its Chairman of the Board for personal reasons. The company also announced that it has appointed Kenneth Bosket, as its Chairman, and is no longer Vice Chairman and Head of Operations. Arnulfo Saucedo-Bardan has been appointed as the company's Chief Operating Officer, and is no longer the company's Secretary. Montse Zaman, has been appointed Secretary, Treasury and Director.
The Board remains at five (5) members.
" I would like to thank Mr. Gewerter for his contributions." stated Mike Zaman, President of EQCO2, Inc.
About EQCO2, Inc.
EQCO2, Inc. (OTCMKTS:CLNO) is in the process of changing its direction of operations to that of a business to business ("B2B") international e-commerce company with an online and mobile optimized platform to facilitate e-commerce between manufacturers and buyers.
This news release may contains "forward-looking statements" as that term is defined in Section 27A of the United States Securities Act of 1933, as amended and Section 21E of the Securities Exchange Act of 1934, as amended. Statements in this press release which are not purely historical are forward-looking statements and include any statements regarding beliefs, plans, expectations or intentions regarding the future. Such forward-looking statements include, among other things, the development, costs and results of new business opportunities. Actual results could differ from those projected in any forward-looking statements due to numerous factors. Such factors include, among others, the inherent uncertainties associated with new business opportunities and development stage companies. These forward-looking statements are made as of the date of this news release, and we assume no obligation to update the forward-looking statements, or to update the reasons why actual results could differ from those projected in the forward-looking statements. Although we believe that any beliefs, plans, expectations and intentions contained in this press release are reasonable, there can be no assurance that any such beliefs, plans, expectations or intentions will prove to be accurate. Investors should consult all of the information set forth herein and should also refer to the risk factors disclosure outlined in our annual report on Form 10-K for the most recent fiscal year, our quarterly reports on Form 10-Q and other periodic reports filed from time-to-time with the Securities and Exchange Commission.
Mike Zaman, President/CEO
EQCO2, Inc.
SOURCE: EQCO2, Inc.
TOPICS CLNOEQCO2OTCMKTS:CLNO
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text:
Generatieve kunst is een vorm van kunst dat in het geheel of deels is gecreëerd met behulp van een autonoom systeem. In deze context is een autonoom systeem doorgaans niet menselijk en kan het onafhankelijk kenmerken van een kunstwerk vaststellen die anders rechtstreeks door de kunstenaar bepaald zouden moeten zijn.

Met de term wordt vaak gerefeerd aan computergegenereerde beelden die algoritmisch zijn ontstaan. Het kan echter ook worden gemaakt met systemen van scheikunde, biologie, mechanica, robotica, smart materials, kansrekening, wiskunde, symmetrie, tessellatie en meer.

Zie ook
 Game of Life
 Interactieve kunst
 Mediakunst
 Systems art
 Systeemtheorie

Kunststroming
Mediakunst

redpajama_set_name: RedPajamaWikipedia