text:
Last Minute Information
Sponsors & Patrons
Calls and Submissions
Camera-ready Papers
Call for Special Sessions
Call for Tutorials
Call for Satellite Workshops
Call for 3 Minute Thesis
Destination Kos
Island Excursions
About Greece
Tutorial Slides
Satellite Workshops
Workshops Program
Industry Workshop
Nisyros Island
Nisyros is one of the most beautiful Aegean islands, still untouched by the tourism growth. It is part of the Dodecanese group of islands, situated between Kos and Tilos. The island extends over a surface of 41 km and its coastline is 28 km long. It can be reached by ferry from Piraeus, Kos and Rhodes.
Greek mythology states that Nisyros was once a part of the island Kos, but during the fight of the Titans, Poseidon plucked a part of Kos and threw it at Polyvotis, sinking him forever in the bottom of the Aegean Sea. This part of Kos, which crushed Polyvotis became Nisyros, and it is believed that when this half dead giant shakes and groans, the volcano erupts. These explosions shaped the island, which is considered to be the youngest volcanic centre in Greece, still active – along with the volcanoes of Milos, Santorini and Methana.
Apart from being the capital of the island, Mandraki is also its harbour. It is one of the biggest villages on Nisyros with lots of tourism facilities available. Enjoy a stroll around the exquisite pebbled narrow streets and squares and take a close look at the white or coloured two-storeyed houses with wooden balconies. The houses are made of volcano rocks and insulated with pumice-stone. The nightlife is not very intense but you will surely have a good time in Mandraki, where there are little tavernas and some bars.
EMPORIOS
Located 8 km east of Mandraki, Emporios stands out for its houses' architecture. It flourished during the 20th century when it had many inhabitants, shops (blacksmith workshops, groceries, tailor-shops, coffee shops) and skilled stone makers. During the '60s a great part of the population moved to the village seaport, Palloi.
NIKIA
Porta is the main square of Nikia, where the church of Eisodion tis Theotokou [Presentation of Virgin Mary] is located; this is one of the most beautiful churches of Greece! The square has an ellipsoid shape and pebbled floor designed by Paschalis Paschalakis, a famous artisan of the 19th century, born in Nisyros. Walking through the village, you will find some nice spots looking at the volcano. Nikia is located 14 km south east from Mandraki.
Sights worth visiting
The volcano
You may walk in the crater and watch the boiling holes. The most recent explosion took place in 1888, shaping a cylindrical pipe of volcano elements, with a diameter of at least 25 m. Polyvotis, the largest crater of the volcano, has a diameter of 260m and a depth of 30m. There are five younger craters, in the same area, the largest being "Stefanos" with a diameter of 30 m and a depth of 30m. The volcano surrounds a valley (caldera) 2,400m long and 950m wide.
Visit the volcanological museum in Nikia, at the edge of the caldera, offering a unique view. It is well-equipped with samples and pictures of rocks, maps, diagrams, photographs, models of volcanoes, computer simulations and much more. Thanks to the technology you will learn about the volcanic activity of the area through three dimensional moving images.
Paleokastro
Α classical period acropolis of the ancient city(6th- 4th century) . It is still in very good condition because of its high quality construction and represents one of the best fortification works of the ancient times, basaltic andesite (volcanic rock), one of the hardest rocks in the world. The main gate is composed by well-hewn stone blocks with a perfect fit of the connecting surfaces. The western and northern part of the hill ends up in a steep and rocky shore which is the natural fortification. The total length of the fortification is very big, as it surrounds the entire land-lubber site of the rocky hill above the south end of Mandraki. Part of the defensive system with towers and observatories that run through Nisyros and the surrounding islets were used to warn through smoke or fire about forthcoming dangers.
The monastery of Panagia Spiliani
, built at the top of a hill northwest of Mandraki. The biggest part of the monastery is built in a cave. The cave is divided into two churches. The north is dedicated to the Kimisi tis Theotokou (the Assumption) and the south, Agios Charalambos (Saint Charalambos).
The Castle of the knights
The Castle of the knights of St. John in Mandraki, built high on a rock at an altimeter of 150 meters. The castle is a reminder of the Knights of St. John having stepped foot on the island and built -in 1315- a magnificent castle in which nestles the Monastery of Panagia Spiliani dedicated to Kimisi of Theotokou (the Assumption).
How to get to Nisyros from Kos island
Basically, there are two scenarios for taking the boat to Nisyros. In the summer months the boats run from both Kos town (Eastern part of Kos) and Kardamena (Southern/Middle part of Kos). If you choose to take the boat from Kos harbour you will spend 1 1/2 hours to get to Nisyros, while if you choose to take the boat from Kardamena, it will take only 1/2 hour to Nisyros harbour. Kos is a small Island and it can be crossed relatively quickly in a taxi. Depending on which scenario you find yourself, the taxi ride to Kardamena is about 30 minutes from Kos town (cost of taxi will be around € 30-40). The destination for all of the Nisyros bound Boats is Mandraki Nisyros. The boats are generally very inexpensive and there is no need to make reservations on the boats.
The thing to remember going back is to check the boat schedule well in advance.
The name of the island probably comes from the pre Hellenic Aegean times. The first inhabitants of Nisyros were the Kares. They were followed by the Achaeans of Kos and the (Doric descendant) Rhodians. According to Homer, the Nisyrians were amongst the Greeks (Hellenes) that fought in Troy. The Nisyrians are also found in the taxation records of the Athenian alliance. They worshipped Apollo of Delphi and Poseidon of Pelasgui whose temple was found in what today is Palos where the old thermal springs were found. Preserved dedicatory columns tell us of the distinguished position held by Zeus, Hermes and many more deities in the daily worship of the inhabitants. The historical fortune of Nisyros is common to other southeastern Aegean islands. The 5th and 4th centuries B.C. constitute the period of the greatest prosperity of the island. At that time, the island thrived on obsidian commerce, extracted by the inhabitants of Nisyros, from the nearby island, Gyali.
During the Median wars, Nisyros paid tribute to Artemisia, to the Queen of Halicarnassus who was sympathetic towards the Persians. Later, and for a short period of time, the Nisyrians joined the ranks of the Athenians. They soon became independent and remained so until 200B.C. when they submitted to Rhodes and whose historical fortune they followed loyally ever since. The Ioannite Knights (Knights of the order of Saint John) fortified Nisyros in 1315A.D. building five forts in strategic positions on the island in the years that followed. In 1422, a huge volcanic eruption formed the large crater in the middle of the island. The Turks looted Nisyros at least four times until 1523 when they subdued it and together with the other surrounding islands, subjugated it to the peculiar privileged regime of the "maktu"(an annual fee that had to be paid). During the Hellenic (Greek) Revolution of 1821, the Nisyrian sailors and their vessels supported the sea operations of the Hellenes (Greeks) but Nisyros was not to be part of the then newly formed Greece. At the end of the 19th century and at the beginning of the 20th, the world wide Nisyrian expatriates provided a lot for the island which was unfortunately conquered by the Italians in 1912. It was in 1913 that the last eruption of the volcano occurred. After the second world war, Nisyros was finally conceded to Greece by the defeated Italians in 1948.
http://www.nisyrosinfo.com/
http://www.visitgreece.gr/en/history/history_of_nisiros
www.nisyros.com (transportation)
http://www.greece.com/photos/destinations/Dodecanese/Nisyros/
www.meetingplanner.gr
Shen xin smiled and didn t talk any more shen wang happened to be eating nearby, and soon when he came, he took shen xin away without. Shen looked at her, I think you just want to be with him, Exam Guide right shen Study Guide Book xin was inexplicably guilty by him anyway, when Braindump I encountered. Impulse ye zhiyou countered, and just engaged first, not that he would get married soon this is already a big concession, but shen wang still. Spit out the water in his mouth you don t want to think about things all day long since you are so careless, you might as well consider how to. Are you two living in the Vce same room walking alone and widows, don t you think traveling is so easy remember to take protective measures. Smiled Exams and exclaimed you look good and suit you although Demo Download I know this is the compliment of the shopping guide, shen xin still have something. Father father shen looked at ye zhiyou with a smile shen was so embarrassed that he was going to die, he just wanted to quickly pull his. Between li muyao and tao haoran clearly jiyue is a big company, but not many netizens know tao haoran this report made them know a new rich. The moon Brain Dumps was bright, ye zhiyou stopped and looked up at the night sky the moon VCE to PDF is so beautiful tonight shen xinchen after hesitated, he added. Me a while to walk back and forth in the starlight park for an entire morning, and this group of shots was not finished starlight park is exam dates 2019 now. Shen xin chose the skirt he wore on his own when he bought it, he felt that the skirt looked good shen xin would definitely look better in it. That the police arrested tao haoran wow, your news is really good shen wang poured himself a glass of water and sneered I also know that all. Mobile phone ST0-199 Exam-Paper-Pdf to the other side to command it every day, of course, it s easy, it s me who hits me, and me who hits me, don t stand still it. Marry him were so straightforward that shen xin still couldn t understand, but thinking of this, ye zhiyou frowned he was too anxious to pick. Shaped black eyes seemed to suck shen shen into her heart, making her heart beat wildly even if he knew that ye zhiyou was drunk, the Exams Prep words. Stay in the industry the biggest suspect is him ye zhiyou snorted coldly and drove to the front row of jian jian check the trends I know. Meant, tonight this meal had to be made Q&A by the two of them she felt an empty stomach and told ye zhiyou let s 642-359 Test-Exam cook first, eat slowly after. Stopped your work I don t know what will happen next please ask for blessings the agent dropped this sentence and turned to leave li muyao s. Young, you guys 9L0-612 Dump-Test the two haven HP0-704 Vce t known each other for a long time after he said this, Brain Demos ye zhiyou s mouth tightened dad shen smiled at him and. Throat two times shen xin, I ordered you to marry me shen xin s brain banged, and he was stunned on Preparation Materials the spot, leaving only ye zhiyou s. Let 000-745 Test-Prep her go to the ward to see ye zhiyou ye zhiyou lived in a single ward at this moment, there was only one nurse standing next to the bed to. Father father shen looked at ye zhiyou with a smile shen was so embarrassed that he was going to die, he just wanted to quickly pull his. Alone, but she Practice ended up and laugh at him I Free m bragging about you shen xin saw that his bowl had a small tip in the heap, and he also offered to. Pulled her into her arms in he has some strength, and sits down when he was on his lap, there was a momentary stun without waiting Brians Dumps for her to. Laughed why, are you so surprised in your heart, are my father and I both the same stubborn old antiques no, I know brother and dad are the. Are such a beautiful girl, it s not safe to be alone even though it was dark early, it was less than eight o clock shen xinzheng said here. Over because of drinking anxious, fast up the face, now ye zhiyou s face has a clear pink, and his body also has a layer PDF of wine he didn t. President ye many stars, let ye always invite one to come and help Study Material us endorsement, shouldn t it be 1Z0-045 Exam-Demo difficult shen shen said we have also. Looking at the jewellery in the counter, I couldn t move it sight I think these are also very good she usually does not bring any precious. Are plans to recruit people and interviews are scheduled today ye zhiyou hooked his lower lip he hasn t kept his mind for the past two days. Such a powerful 310-084 Exam-Vce romance boy help http://www.examitpass.com/ shen xin make the shape this team will Practice let you also pack your things and walk out of the villa it was. Asking, what exactly do you want to do she just didn t he laughed at him for a moment is he going to get revenge ye zhiyou held hands, took. He drank some wine at the party the day before yesterday , 1Y0-222 Practice-Exam-Pdf although a bit drunk but remember what happened he didn t believe that the words. She said something reasonable it seems to be the case, so let s get engaged first shen xin no, the gradual step she said was to fall in love. Bad here ye zhiyou said, well, what do you think of when we move here after we get married attentive there is also a trend of red faced ears. A have long heard Study Material of that zhizhi you who fled 350-001-LAB-CN Pass-Exam when I came back to inherit the company, I wanted to take a look at him today, and many people. You want to do you better not mess around there is monitoring here, and no one should be in trouble I want to run those people seemed to be a. Ouyang tian the same day a message was sent, asking him to bring the relevant materials with him the next Exams Material day to apply for employment the. He 24 hours Pdf had a car accident, but it didn t work out li yantang smiled you ran to my house come on xie kaihuai was speechless, goodbye I m home he. Opened the door and walked out ye zhiyou stood next to the car, heard the sound of exam opening the door, and looked in the direction of heart past. People would affect the patient s rest now that the police are here, let s go back and come back tomorrow morning, while still giving ye. Even chat no ye zhiyou answered categorically, I don t like him shen shen said coincidentally, neither am I like him ye zhiyou glanced at her. He Study Guide Book reported on shen xin s favorite hotpot rest assured with him, after all, hot pot was a favorite thing he immediately took a taxi and took. Her, don t even want to run in short, if you encounter this kind of thing, don t go up alone, just give me it call alright ye zhiyou said that.
Copyright EUSIPCO 2017


Before sending correspondence to the Minister, please ensure you are familiar with the following policy.
We aim to read all correspondence within one business day of receipt, and respond where required, within 20 working days of receipt.
Where your correspondence results in action being taken to resolve your issue, the Department may contact you by telephone or email. No formal response from the Minister will be provided.
A common sense approach is applied when preparing correspondence. All comments are noted, but may not result in a formal response.
We will only respond to items where the Minister is the primary recipient. We will not respond to correspondence where the Minister is not the primary recipient, but is marked as carbon copy.
Petitions can only be officially presented by Members of the Legislative Assembly. Petitioners should contact their local Member of Parliament to ask for the petition to be lodged. Find more information about petitions on the Parliament NSW website.
We will forward any correspondence containing threatening content or advocating illegal activities to the NSW Police Force.
Your correspondence, once received, will become a formal government record. We will treat this with the appropriate level of confidentiality consistent with the Privacy and Personal Information Protection Act 1998.
Read the latest media releases about transport and roads on the Transport for NSW website.


The Grand Finals of the 4th Steinway Youth Piano Competition 2018 will take place on June 30 at the Carlos P. Romulo Auditorium at the RCBC Plaza.
The Grand Finals will see 16 young aspiring pianists from ages 8 to17 vie for a chance to represent the Philippines at the Regional Finals in Taiwan.
This year, the Philippines fields sixteen national finalists: Selina Go, William Matthew Lim, Angelmay Tsai, and Eunice Cai Yang for Category 1 (ages 11 and below); Patricia Chloe Bangco, Athena Justina Elizaga, Nathan Go, Catherine Li, and Sophia Dominique Sumulong for Category 2 (ages 12 to 14); and Eimee Alarcon, Gregory Cheong, Faye Jasmine Dinglasan, Kirsten Chantrice Fabellar, Rueben Kiel Ibon, Irene Jasmine Lee, and Andrea Bernadette Verzosa for Category 3 (Ages 15 to 17).
The Grand Finals will be judged by Steinway Artists Professor Benjamin Loh (Singapore) and Dr. Eleanor Wong (Hong Kong), as well as Professor Albert Tiu who is a renowned Filipino pianist based in Singapore.
The winner of Regional Finals in Taiwan will represent Southeast Asia at the International Steinway Festival 2018 in Hamburg, Germany.
"As we are committed to providing a holistic environment in nurturing young talents like yourselves, it is also a natural extension of our business to create this opportunity for young budding musicians to showcase their talents on a platform that is modelled after the Hamburg Festival which is a non-competitive musical get-together aimed to help foster friendships in celebrating the joy of music-making," adds Celine Goh.
The Philippine Steinway Youth Piano Competition will be held on June 30, 2018, Saturday, 8:30 AM at Carlos P. Romulo Auditorium in RCBC Plaza, Ayala Ave., Makati City. For tickets, please visit Steinway Boutique Manila at Level 1, Shangri-La Plaza East Wing, Mandaluyong City or contact us at info@steinway-boutique.com.ph | +632 637 7600.
5 Nate Ruess Songs That We Can't Wait to Hear At His Concert Tomorrow!


Digicity is a Information Communication and Technology company in Malaysia. We are expert in supply, deploying, delivering and maintaining many type for ICT product. .
Every high-growth business grapples with decisions around the best business system to manage its expanding operations. We will helping companies seek ways to accomplish high performance in their business operations through System Software.
We are highly capable in doing System Installer within Central and Southern Region of Malaysia. We got several expert technician that will make all the task complete by the dateline. All the training that are needed by our client will be made by our expert instructor. All the module for the training is made by the expertise and also principle.
Copyright © 2017 www.digicity.com.my. All rights reserved.


How does the 90-day free trial work?
If you cancel a paying plan within 90 days of signing up you won't be charged a thing . If you do choose to cancel your account in the future you will never be charged again, but you are responsible for charges already incurred up until your cancellation . Refunds can not be issued after your initial 90 day trial and we don't prorate for partial months.
Do I get a 90-day free trial if I upgrade?
If you upgrade from a free account to a pay account you are not eligible for the 90-day free trial. The 90-day free trial on paying plans only applies if you sign up for a paying plan now. You can always downgrade later if you choose.
No. The prices you see above are all inclusive. Every Limocart plan includes specific number of dispatchers and this is explained in the price table. Only "Large Business" plan includes unlimited dispatchers.
How many vehicles I can register from my fleet?
It depends on your service plan. For example if you choose "Small Business" plan you can only register 15 vehicles from your fleet. If you have more vehicles in your fleet please see the price table for higher options.
Currently we accept Visa, Mastercard, American Express and Discover. We do not accept PayPal. At this time we only accept payments online so we will not be able to accept a P.O., invoice you, or take an order over the phone.
Our state-of-the-art computer servers are protected by biometric locks and 24-hour surveillance. Our product software and infrastructure is updated regularly with the latest security patches. Our network is protected by an enterprise-class firewall and all Limocart plans include SSL encryption to keep your data safe.
What web browsers work with Limocart?
If you have questions about Limocart or the sign up process just submit a support request and we'll get right back to you .
1. This Agreement (the "Agreement") is a legal agreement that governs your relationship with Limocart.com, Inc., use of the Limocart network website ("Site") and the services available on Site ("Services").
a. In addition to the terms and conditions in this Agreement, users may be asked to agree to additional terms when using different parts of Site. The terms and conditions of this Agreement, along with such additional terms and conditions, will be referred to as the "Terms". Limocart.com reserves the right, at its sole discretion, to change, modify, add, or remove portions of any of the Terms at any time. Please check the Terms periodically for changes. Your use of Services after Terms are posted will mean that you accept all Terms.
b. You shall be referred to as member Limousine Service Partners ("LSP"), and all of your d/b/a's, affiliates, agents, employees, representatives, subsidiaries, locations and subcontractors as "You" or "Service providers." Limocart.com, Inc. and its employees and agents shall be referred to as "We" or "Us" or "Limocart.com." References herein to the Limocart network web site include any and all web sites now, or hereafter, owned or operated by the Limocart network.
c. Limocart LLC is a corporation registered in the state of Delaware.
a. We receive reservations from our customers ("Customer") on your behalf for certain services. In turn, Limocart will send you a communication about Customers' expressed service request need ("Reservation"). We will also send Your contact information to the Customer, along with Your terms and conditions of service. The information We provide about You will be based on the information You submit to Us on our Site (which You may amend at any time) and Customer Reviews, and may further be amended by Limocart at any time. If You do not upload your terms and conditions to Limocart, or provide it to Us, then Limocart's terms and conditions will control and govern your relationship.
b. We do not guarantee to provide You any specific number of Reservations. We are not involved in, nor do We have any responsibility for Your contracts with Customers, their creditworthiness, or any payments to You or any disputes that may arise from Your relationship with said Customers. You hereby agree that Limocart network is free to contract with other service providers or partners, as this is not an exclusive contract.c. We shall provide Customers the ability to rate their experience working with You via Customer Reviews and We may disclose such ratings publicly. Limocart network will request Customers to provide quality feedback evaluating their experience with each LSP. Survey questions include but are not limited to the following: Positive, Negative or Neutral as well as an open field for customer feedback. The results of these reviews will be shared publicly on Limocart. Any service issues identified via low quality ratings may result in termination of this Agreement or require Limocart to develop a formal plan to prevent the future occurrence of such issues.
d. Limocart shall host the System, Site and all pages relating to the Services as part of the Services. However, LSP is solely responsible for all content provided that appears on Service providers and partners web pages and on the Services.
e. In the event that Your customer rating is less than seventy-five percent (75%), Limocart reserves the right in its sole discretion to suspend Your availability until You and Limocart have agreed to required changes to reinstate Your account. If You do not wish to have Your availability reinstated, You must advise Limocart about termination. Your account will incur costs until such termination request is received.
i. You are licensed, insured, qualified and capable of performing any of the services You have provided to Us or selected via the Site (including, but not limited to, vehicles, airports served, service types and service areas) and any subsequent updates You provide to us. ii. You are, and at all times shall be, properly and fully licensed, bonded and insured at levels in accordance with applicable governmental, regulatory and industry standards under all applicable laws and trade regulations. You agree to produce documentation to verify this to Limocart. Further, You agree that You have not been turned down for insurance coverage related to Your provision of services. You will notify us promptly of any changes to your licensing, bonding or insured status. If You or Your company is operating under a license with special restrictions, You agree and represent that You will at all times operate within the guidelines as specified by such restrictions.
iii. You will operate any reservation You receive from Limocart as if it was generated by You. You will be entirely responsible for each and every job booked through Limocart and the full execution of each job.iv. You will comply with the Limocart registration requirements as specified from time to time, including providing Limocart network with any updates or renewal documentation of licensing and insurance coverage. Each applicant to Limocart must complete and submit a registration application on-line at www.Limocart.com. Upon receipt of Your registration, Limocart will notify You of your activation.
v. You hereby represent that You, any predecessor entities of Your company, any other majority shareholders, partners or members, and Your company, are free from any of the following: (i) bankruptcies or civil legal judgments within the last 3 years, or (ii) felony criminal convictions, or You have provided Limocart with an accurate and truthful written statement detailing any exceptions. If at any time during your membership with Limocart network, You, any predecessor entities of Your company, any other majority shareholders, partners or members of Your company should file for bankruptcy or be involved in bankruptcy proceedings, have a civil legal judgment entered against such party or a felony conviction entered against such party, You will promptly notify Limocart. You further represent that You have not been sanctioned or penalized by any governmental authorities in connection with Your provision of services to any of Your customers and that You have not been denied membership to (or had Your membership revoked from) any professional industry associations. You authorize Limocart to verify that all of the above representations are truthful and accurate.
vi. You represent and warrant that You will perform Your services for Customers in a good and workmanlike manner consistent with the industry standards. You will fully cooperate with Us if We attempt to facilitate the resolution of any Customer complaints between You and Your Customers. However, You hereby agree and acknowledge that Limocart is not liable to You or any Customer if such matters cannot be resolved. You agree that We shall have the right to disclose any information We have regarding You and Your company to any authorities requesting information from Us regarding any work or services You have performed.
vii. If You provide written comments or testimonials about Our services or activities, You agree that Limocart shall have sole ownership of any and all intellectual property rights in such comments or testimonials, and that We may post and publish Your comments or portions thereof at Our sole discretion on Our Sites or in marketing materials including Your name and company or agency, and that You shall not be entitled to any payments associated with Our use of the foregoing. You hereby authorize Us to use Your name, company name, and franchise name, as applicable, and the use of any trademarks, service marks, trade names and logos, in the form or format that You supply to Us or that You upload to Our Sites, for use and posting on Our Sites and for use in marketing materials to be presented to Customers in online postings, via emails or otherwise to help promote You or Your services. You shall not be entitled to any payments associated with Our use of the foregoing. Limocart reserves the right, in its sole discretion, to review, reject and remove any content that You upload to the Limocart network Sites or Your company profile. You further represent that You have all necessary rights to display any of the logos, service marks, trademarks, and any other content that You upload to our Sites, and You represent that Your uploading for display of any such content, and the use by Limocart of such content as contemplated by this Agreement, shall not violate any third party's intellectual property rights. If there are any limitations or restrictions pertaining to the use or presentation of such logos, trade or service marks, it shall be solely Your responsibility to provide Us in writing with any such restrictions or limitations of use. If expressly authorized by Us in writing in advance, You may be authorized during the term of this Agreement to use certain marks of the Limocart network as necessary to promote Your business. You are expressly prohibited from registering any trademarks or domain names of Limocart.com.
viii. You acknowledge and agree that all of the content and information posted on the Site, including but not limited to Service providers and partner profiles, screening information and Customer Ratings & Reviews for all Limocart LSP members (excluding any logos or trademarked materials, or other intellectual property of Service providers and partners provided by Service providers and partners), is the sole and exclusive property of Limocart. You acknowledge and agree that You have no right to reproduce, post, publish, display or otherwise use any Customer Reviews (including those relating to You and your business) , or any other content posted on the Sites, other than content provided directly by You or as otherwise specifically authorized by Limocart. You acknowledge that a violation of the foregoing could result in significant damages and You hereby agree that You will indemnify Limocart.com in the event of any third party claims against Limocart based on or arising from Your violation of the foregoing. You acknowledge and agree that Limocart has the right, in its sole discretion, to contact Customers about services You provide to gain their feedback and rating of You, and to display such Customer Ratings and Reviews on our Sites. Limocart is not responsible or liable to You for any comments, ratings or communications of any kind from Customers that Limocart chooses to post on our Sites.
1. Incorporating any fee in a Customer's bill for any fees paid to Limocart; 2. Charging Limocart customers any more than your other Customers; or 3. Disclosing any Customer personal information to any third party. You will at all times be in full compliance with all applicable federal, state, provincial, local and other laws and regulations that apply to Your activities. You acknowledge and agree that to knowingly share, distribute, transfer, or sell a Limocart Reservation (or the information contained therein) in any way other than expressly set forth in this Agreement, is a serious breach of contract and constitutes fraud. You acknowledge and agree that such a breach by You could result in invasion of privacy rights of others, significant costs and damages to others, and to Limocart and other LSP members. You acknowledge that there may be regulatory fines and penalties imposed on You for contacting consumers and businesses in a manner not in accordance with applicable laws and regulations, including, but not limited to, federal, state, and provincial do-not-call regulations, as well as federal, state and provincial anti-spam regulations. Accordingly, if You knowingly share, distribute, transfer, or utilize a Booking (or the information contained therein) other than as expressly set forth in this Agreement, You agree to fully indemnify and be liable to Limocart as set forth below, for all damages, whether direct or indirect, punitive and consequential, and any regulatory or judicial fines or penalties that may arise from such activities.
x. You agree to allow only employees of Your company or independent contractors performing services directly on behalf of Your business to contact or provide any service to Customers You receive from Limocart. To the extent You use any such subcontractors, You shall be responsible and liable for all acts and omissions of such subcontractors and for ensuring that such subcontractors comply with all the provisions of this Agreement. You agree not to sell, trade, gift, assign, or otherwise transfer any Reservations provided by Limocart to any other party, including any other Limocart network Service provider or partner.
Furthermore, all Service providers and partners must have a garage location or base of operations within the service area You establish on our Site.
xi. You agree that You shall provide prices to Limocart for uses that are consistent with Your current market wholesale rates. Limocart will work to secure Channel Partners ("CP") which will require up to a Fifteen Percent (15%) commission of the base fare less the deposit to CP but withheld by Limocart (based on sales volume), which We will withhold and then We will pass through the balance to the service provider along with the transaction fee when said job is generated from CP's site. The commission applies to the balance after the booking fee.
i. There are four (4) groups in the Limocart network community that work together to create a beneficial match between Customers seeking services and qualified local operators: (1) Customers; (2) service providers and partners; (3) the Limocart network team; and (4) Channel Partners.
1. Act with integrity and mutual respect. Commitments between Customers and Service provider and partners will be clearly stated and kept. Service provider and partners agree to keep appointments and Reservations.2. Communicate Openly. Communication between Customers and Service providers and partners is to be timely, candid and simply stated. Concerns are to be expressed openly and objectively so that, whenever possible, conflicts may be discovered early and resolved quickly.
3. Share Information. All Customers should be encouraged to contribute to Reviews. The reviews are intended to expand the power of "word of mouth" advertising to promote service provider and partner capabilities and encourage continuous improvement. All service Reservations will be monitored and evaluated to provide a medium in which each of the three groups comprising the Limocart community may obtain their intended benefits.
4. Problem Resolution. If requested by either a service provider or partner or Customer, Limocart may offer programs that are designed to assist Customers and service providers or partners in resolving problems that arise between them. Service providers and partners commit to addressing any and all issues between service providers and partners and Customers in a manner consistent with the Limocart resolution process as may be in place from time to time.
5. Respect Privacy. Service providers and partners should take reasonable steps to protect all personal and confidential information shared by Customers while facilitating Services.
6. SP agrees not to initiate any contact with CP regarding any jobs that arise from their relationship with Limocart.
i. Represent themselves and Limocart in a professional manner, including the quality of service or work, professional appearance and superior service.
ii. Treat Customers with courtesy and respect.
iii. Be punctual for Customer reservations and proactively contact Customers when a possible delay in a reservation is known to the LSP. You agree to arrive at least ten (10) minutes before any Reservation.
iv. Return phone calls in a timely manner.
v. Provide Customers with detailed and complete information with respect to the operated job in question, if applicable.
vi. Honor and fulfill all Reservations received from Us.vii. Encourage Customers to participate in the Review process related to their service request.
viii. Participate in the Limocart resolution process for Customer disputes as necessary.
7. You expressly acknowledge and agree that there is no guarantee whatsoever in the amount of work that will be provided to You by Limocart.
i. Reservation transaction fee of $4.99 per transaction. Limocart fees are non-refundable. There is also a Booking Fee charged to the Customer based on the car class selected by the Customer; this Booking Fee is also non-refundable.
ii. There are no activation fees, minimum volumes, or term limits for Your Limocart network account.
iii. Termination: If You wish to terminate this Agreement, You must provide us notice in writing via fax to 201-706-7716. Upon receipt by Us, Your account will be closed within Thirty (30) days of Your last reservation.iv. You acknowledge that it is solely Your responsibility to ensure that the communication methods You have selected in Your Limocart profile are kept current and accurate. Limocart shall not be responsible, or liable, for undelivered Customer notifications. If Your account information is lost or stolen or You suspect that Your Limocart account has been accessed without Your permission, please contact our Provider support team immediately by electronic mail at customerservice@Limocart.com. You remain liable for all Limocart network fees until You contact Limocart.
v. You may view a statement of Your monthly jobs operated on Your Limocart account, in the "My Company" tab of your online account. Limocart will process Your billing via Your selected payment method.
vi. You agree to electronic delivery of Your account activity records, disclosures and other information relating to Your account and the Services provided by Us in connection with Your account. You agree that electronic delivery of these documents will constitute sufficient delivery to You and be deemed received by You when sent, whether or not You actually access or view the message. You understand that it is Your responsibility to promptly and carefully review any messages made available to You electronically or via the Site and to immediately notify Us of any errors. You may change your primary e-mail address, phone number or other contact information by selecting the appropriate item from the "My company tab "section of the Site after logging in. You agree to maintain an active primary e-mail address for log-in and receipt of Bookings and other messages. You consent to receive periodic advertising to Your email address from Limocart advertising sponsors. In the event of failure of one or more of these additional addresses, e-mail notifications will be sent to Your active primary e-mail address until You update Your e-mail addresses. If You elect to stop receiving emails from Us at any time, then You agree to delivery of Your account/billing records and/or Bookings on the Site under your "My Info" section.
vii. No fee is due or payable to the extent such fee is in violation of any applicable law.
a. Each party represents and warrants that it has the right, power and authority to enter into this Agreement, to grant the rights granted herein and to perform the duties and obligations described herein. You represent and warrant that every person who shall perform services for or on behalf of You in connection with this Agreement is at least eighteen (18) years old as of the date hereof.
i. You shall fully protect, indemnify and defend Limocart and all of its agents, officers, directors, shareholders, suppliers, partners, employees and each of their successors and assigns ("Indemnified Parties") and hold each of them harmless from and against any and all claims, demands, liens, damages, causes of action, liabilities of any and every nature whatsoever, including but not limited to personal injury, intellectual property infringement, fraud, deceptive advertising, violation of any state, provincial or federal laws or regulations, property damage, attorneys' fees and court costs, arising in any manner, directly or indirectly, out of or in connection with or in the course of or incidental to any of Your work for or dealings with any Customers, or Your services, representations, advertising, or obligations (including, but not limited to, Your obligation to maintain the confidentiality of user personal information) as provided in this Agreement, including any extra work, regardless or cause or fault or negligence of Limocart or the Indemnified Parties and without regard to cause or to any concurrent or contributing fault, strict liability or negligence, whether sole, joint or concurrent, active or passive by Limocart or the Indemnified Parties.
i. You hereby acknowledge and agree that Limocart services are provided to You on an "as is" basis, and Limocart disclaims any and all express or implied warranties, including, but not limited to, any warranties of merchantability, fitness for a particular purpose, and noninfringement, to the maximum extent permitted by law.
a. Updates or Amendments. This Agreement and the Limocart Fees and Payment and activity records may be changed from time to time and You agree to be bound by any changes that are posted on the Limocart Sites or which are otherwise communicated to You.
b. Confidentiality. Limocart and the service providers and partners agree that at all times during the effectiveness of this agreement, and for two (2) years after the agreement is terminated, each shall keep confidential all information provided by any party in relation to this agreement or the commitments that it sets forth. Such information shall include, without limitation, this Agreement, all Customer information, information regarding other service providers and partners participating in the System and any and all technical or non-technical information provided by any party.
c. Termination. Limocart, in its sole discretion, reserves the right to suspend Your rights under this Agreement or terminate this Agreement and Your membership in Limocart immediately and at any time for any reason or no reason at all. You may terminate this Agreement upon our receipt of your written notice during normal business hours (such termination to be effective upon the close of business on the day properly delivered and received), but You shall be obligated to pay for any Bookings or fees for other services delivered to You prior to Limocart receiving Your termination during normal business hours.
d. Survival. If this Agreement is terminated or expires, Your agreements and obligations under this Agreement shall continue with respect to any Services provided to You during the term of this Agreement and relationships You enter into or create from those Services. Further, Your will continue to be governed by this Agreement, including but not limited to, the respect to any disputes that may arise or claims that may be made against Limocart and any indemnification of Limocart by You for such claims.
e. Legal Rights. If it is determined or suspected by Limocart, in its sole discretion, that You are misusing or attempting to misuse or circumvent the Limocart services or system or any Customer data, or are using or attempting to use it for any inappropriate, illegal, harmful, or anticompetitive purposes, including but not limited to activities such as hacking, infiltrating, fraud, advertising, jamming or spamming, or any activities inconsistent with the services proposed to be offered by Limocart, in addition to our right to immediately terminate this Agreement, Limocart reserves the right, in its sole discretion, to initiate, without notice, appropriate legal actions or proceedings to seek appropriate remedies and/or damages, including but not limited to, lost revenue, repairs, legal fees, costs and expenses, and to seek injunctions or other equitable remedies.
f. Refunds. In the event of termination of this Agreement, You shall not be entitled to any refund of any fees paid to Limocart.
g. Attorneys Fees, Costs. You will be liable for any attorneys' fees and costs if Limocart has to take any legal action (including the retention of a lawyer) to enforce this Agreement. This Agreement, the Terms, the Service and any modifications thereto may not be assigned or in any way transferred without the prior written consent of Limocart. The terms of this Agreement shall be construed in accordance with the substantive laws of the State of New Jersey. Any dispute, controversy or claim arising out of or relating to this Agreement or the breach or termination hereof shall be settled by binding arbitration in Bergen County, New Jersey, U.S.A. h. Independent Contractors. The relationship between Limocart and You shall be that of independent contractors and neither party shall be or represent itself to be an agent, employee, partner or joint venture of the other, nor shall either party have or represent itself to have any power or authority to act for, bind or commit the other.
i. Survival of Terms. If any provision of this Agreement is declared invalid as contrary to law or public policy, the remaining provisions hereof shall continue to remain in full force and effect.j. Notice. Except as explicitly stated otherwise, any notices to Limocart regarding this Agreement shall be sent to customerservice@Limocart.com. Any notice to a user shall be given to the name and address submitted at the time of registration.
k. No breach by either party hereof shall be deemed material unless the other party shall give written notice of such purported breach to the breaching party and the breaching party has not cured such breach within seven (7) business days after receipt of such notice.
l. This Agreement shall not be deemed to create any joint venture, partnership or agency between the parties hereto. It is understood that each party to this Agreement shall be independent of the other and that neither party shall have the right or authority to bind the other party.
m. Disclosure: The Service hereunder is offered by Limocart.com, Inc., 115 River Road, suite 1206, Edgewater, NJ 07020. n. You hereby agree and acknowledge that Limocart is strictly a platform for Your bookings. You acknowledge and agree and that Limocart is not, nor in any will be held, responsible for any loss, damage or problem that You encounter with any of the customers you gain from Us. Limocart is a technology company that brings You and the customers together. You agree to use Limocart at your own risk.
o. This Agreement constitutes the complete agreement between the parties hereto on the subject matter hereof, and all prior or contemporaneous agreements between the parties, whether oral or written, shall be deemed merged herein.
Give us enough notice so that our drivers aren't already committed, and there's no fee.
Cancellations that occur at less than these time frames will result in a charge of the full base fare as referenced on all trip confirmations sent via email for every reservation.
Additionally, in the event the reserved base fare exceeds $500.00 (or USD equivalent), the minimum cancellation fee is 40% of the base fare irrespective of when the reservation is cancelled.
For special events and/or specialty vehicles, the reservation is cancelled within 24 hrs. of the service date there is a 100% cancellation charge. All cancellation notices within 24 hrs. must be given via email in writing to reservation@limocart.com.
It's highly recommended to attach your company's certificate of insurance below. Optionally you can fax it to us at 1-201-706-7716 if you don't have it on your computer . You won't be able to receive Limocart reservations without certificate of insurance.
I have read and accepted the Affiliate Terms and Conditions for this website.
© 2019 Limocart | Your Global Car Service | All rights reserved.


One of my projected sorrows when moving last summer from a manufactured home with a lovely yard to an apartment complex was the loss of my flower gardens. However, that unnecessary anxiety was erased this summer by the beautiful, luxuriant potted plants that fill our balcony.
They were easy to plant (after I lugged 6 bags of Miracle Gro potting soil and all the plants up the stairs), require no weeding, take 5 minutes to water (if there is no rain), and are colorful reminders of God's beautiful world.
I had to agree. They are happy here. And why not? They have plenty of water, enough food for the growing season, sunlight – and most important, a lot of love! What else could they want?
I thought about that question throughout this week. Is human happiness that easy to find? The same Creator provides and cares for me. I currently have everything I need to keep my body as healthy as it can be in this season. I have the Light of the World available to me at all times. I have the love of family and friends to encourage my growth. To quote a cliché, I have learned to "bloom where I am planted" just as my petunias, daisies, marigolds, geraniums, delphinium, and begonias have. We can learn a lot about the grace of God just by looking around us.
This entry was posted in Living as Apprentices and tagged being happy, gardens, God's love, grace. Bookmark the permalink.


Defender Chris Baird admitted the "time was right" to call time on his international career.
Baird has confirmed that he was hanging up the Northern Ireland jersey for good after winning 79 caps.
The 34-year-old – who was a member of this summer's historic Euro 2016 squad – brings the curtain down on a loyal 13-year stint with his country in order to focus on his club career with Derby County.
Baird made his debut against Italy in 2003 and ended it against Poland in this summer's Euros.
He told the Irish Football Association website: "It was a tough decision because I have enjoyed every single minute that I have played for Northern Ireland.
"I discussed it with my family and as I am not getting any younger I felt it was the right thing to do at this stage.
"Qualification for Euro 2016 was the icing on the cake for my international career, but I have so many fantastic memories, like the win over England and beating Spain.
"I am fortunate to have lined out with some great players over the past 13 years and I would like to thank the managers, coaches and Irish FA staff that I have worked over that time.
Baird – who has played at right-back, centre-back and more recently as a defensive midfielder for Northern Ireland – lined out in nine of the 10 qualification games that took Michael O'Neill's side to Euro 2016.
The former Fulham star was a pivotal player for O'Neill as Northern Ireland reached their first-ever European Championship finals.
His international manager described the versatile midfielder as a "rock" during the stellar qualifying campaign, which saw Northern Ireland lose just one of their 10 games to finish top of their group.
"Chris has been an absolute rock for me in the four years that I have been in charge," O'Neill said.
"His defensive qualities, his reading of the game and his experience have been invaluable, especially in our EURO 2016 qualification campaign.
"In addition to his football ability, he is also a fantastic person and we will miss him around the squad.
"Chris has been a tremendous servant to Northern Ireland.


text:
Route 11 Bypass or Highway 11 Bypass may refer to:
 US 11 Bypass
 North Carolina Highway 11 Bypass

See also
List of highways numbered 11
List of highways numbered 11 Business
List of highways numbered 11A
List of highways numbered 11B
List of highways numbered 11C

redpajama_set_name: RedPajamaWikipedia


text:
Q: Removing last item from ListView crashes the app I am creating an Alarm Clock which has a problem that every time I remove last item of list view my app crashes I can't figure out how to fix even after googling and banging my head.
This is my SQLitehelper code
    public int deleteAlarm(long id) {
    return getWritableDatabase().delete(Alarm.TABLE_NAME,
            Alarm._ID + " = ?", new String[] { String.valueOf(id) });
}

AlarmManagerHelper.setAlarms
public static void setAlarms(Context context) {
    cancelAlarms(context);
    AlarmDbHelper dbHelper = new AlarmDbHelper(context);
    List<AlarmModel> alarms = dbHelper.getAlarms();

    for (AlarmModel alarm : alarms) {
        if (alarm.isEnabled) {
            PendingIntent pIntent = createPendingIntent(context, alarm);

            Calendar calendar = Calendar.getInstance();
            calendar.set(Calendar.HOUR_OF_DAY, alarm.timeHour);
            calendar.set(Calendar.MINUTE, alarm.timeMinute);
            calendar.set(Calendar.SECOND, 00);

            final int nowDay = Calendar.getInstance().get(
                    Calendar.DAY_OF_WEEK);
            final int nowHour = Calendar.getInstance().get(
                    Calendar.HOUR_OF_DAY);
            final int nowMinute = Calendar.getInstance().get(
                    Calendar.MINUTE);
            boolean alarmSet = false;

            for (int dayOfWeek = Calendar.SUNDAY; dayOfWeek <= Calendar.SATURDAY; ++dayOfWeek) {
                if (alarm.getRepeatingDay(dayOfWeek - 1)
                        && dayOfWeek >= nowDay
                        && !(dayOfWeek == nowDay && alarm.timeHour < nowHour)
                        && !(dayOfWeek == nowDay
                                && alarm.timeHour == nowHour && alarm.timeMinute <= nowMinute)) {
                    calendar.set(Calendar.DAY_OF_WEEK, dayOfWeek);

                    setAlarm(context, calendar, pIntent);
                    alarmSet = true;
                    break;
                }
            }

            if (!alarmSet) {
                for (int dayOfWeek = Calendar.SUNDAY; dayOfWeek <= Calendar.SATURDAY; ++dayOfWeek) {
                    if (alarm.getRepeatingDay(dayOfWeek - 1)
                            && dayOfWeek <= nowDay && alarm.repeatWeekly) {
                        calendar.set(Calendar.DAY_OF_WEEK, dayOfWeek);
                        calendar.add(Calendar.WEEK_OF_YEAR, 1);

                        setAlarm(context, calendar, pIntent);
                        alarmSet = true;
                        break;
                    }
                }
            }
        }
    }
}

any help will be gratefully received.
LogCat
05-24 02:47:33.640: E/AndroidRuntime(819): FATAL EXCEPTION: main
05-24 02:47:33.640: E/AndroidRuntime(819): Process: com.b.alarmclock, PID: 819
05-24 02:47:33.640: E/AndroidRuntime(819): java.lang.NullPointerException
05-24 02:47:33.640: E/AndroidRuntime(819):  at com.b.alarmclock.alarm.AlarmManagerHelper.setAlarms(AlarmManagerHelper.java:31)
05-24 02:47:33.640: E/AndroidRuntime(819):  at com.b.alarmclock.alarm.AlarmClock.deleteAlarm(AlarmClock.java:64)
05-24 02:47:33.640: E/AndroidRuntime(819):  at com.b.alarmclock.alarm.AlarmClock$AlarmListAdapter$2.onLongClick(AlarmClock.java:178)
05-24 02:47:33.640: E/AndroidRuntime(819):  at android.view.View.performLongClick(View.java:4474)
05-24 02:47:33.640: E/AndroidRuntime(819):  at android.view.View$CheckForLongPress.run(View.java:18401)
05-24 02:47:33.640: E/AndroidRuntime(819):  at android.os.Handler.handleCallback(Handler.java:733)
05-24 02:47:33.640: E/AndroidRuntime(819):  at android.os.Handler.dispatchMessage(Handler.java:95)
05-24 02:47:33.640: E/AndroidRuntime(819):  at android.os.Looper.loop(Looper.java:136)
05-24 02:47:33.640: E/AndroidRuntime(819):  at android.app.ActivityThread.main(ActivityThread.java:5017)
05-24 02:47:33.640: E/AndroidRuntime(819):  at java.lang.reflect.Method.invokeNative(Native Method)
05-24 02:47:33.640: E/AndroidRuntime(819):  at java.lang.reflect.Method.invoke(Method.java:515)
05-24 02:47:33.640: E/AndroidRuntime(819):  at com.android.internal.os.ZygoteInit$MethodAndArgsCaller.run(ZygoteInit.java:779)
05-24 02:47:33.640: E/AndroidRuntime(819):  at com.android.internal.os.ZygoteInit.main(ZygoteInit.java:595)
05-24 02:47:33.640: E/AndroidRuntime(819):  at dalvik.system.NativeStart.main(Native Method)

A: If you have a NullPointerException on the line
for (AlarmModel alarm : alarms) {
then alarms must be null. Either you should do a null check before the for loop or make sure dbHelper.getAlarms() always returns a non-null List, even if it's empty.

redpajama_set_name: RedPajamaStackExchange


text:
Two Gung-Ho Nuclear Engineers Brave the Unknown to Clean Up Three Mile Island
By Greg Walter
Volunteers Only—that was the order at the Three Mile Island nuclear plant in Middletown, Pa. when the Metropolitan Edison Company needed two engineers to inspect the damage done in the most serious U.S. industrial nuclear accident. Although most of the radioactive krypton gas had been vented into the atmosphere by last month, the Unit 2 containment building was still "hot." How hot, and how deadly, no one could say for sure until engineers Bill Behrle, 36, and Mike Benson, 27, courageously offered to enter the building.
Their first attempt was canceled when the safety equipment failed to meet standards, and the second ended when the 1,000-pound steel door to the building stuck. It was subsequently drilled open, and Berhle and Benson prepared for a third foray. "I wanted to play some meaningful role in the company's recovery of Three Mile Island," says Behrle, who has been with General Public Utilities, the parent of Metropolitan Edison since 1967. Although they received no extra pay for the mission, the men admit that there were other reasons they stepped forward. Benson, a nuclear engineer who was hired by Met Ed on April Fools' Day 1974, has been frankly bored with the damage-review desk work he has been doing since the accident 16 months ago. Behrle, one of the company's top engineers, is a thrill lover who plans to learn skydiving next.
Swathed in three layers of cotton coveralls topped by firemen's coated clothing, Behrle led his colleague through an air lock and opened the door to Unit 2. He held out a Thermal Luminescent Dosimeter (TLD), studied the dial—and found a remarkably low level of radiation. "As soon as I got my first reading of 400 millirems, I felt like a kid in a candy shop," he recalls. Although another radiation measuring device registered 22,000 millirems for an instant when he extended it toward the water surrounding the reactor core—the hottest place in the building—Behrle learned when he left the building 20 minutes later that neither he nor Benson had been exposed to more than 220 millirems an hour—well within the federally imposed safety limit of 3,000 millirems during a three-month period. The engineers returned with smear samples of surface contamination and took photographs. Met Ed plans five more entries to map the damage before beginning repairs.
Met Ed officials say the pair pulled off the job flawlessly, but Behrle's performance at the press conference afterward was not as well programmed. Asked to describe what he had seen, he replied, "Well, it's kind of like entering a tomb." That caused an uproar. Behrle went on to explain, "These places are damp and muggy, and I always equate them with catacombs. I didn't mean it was eerie or foreboding." Even during a dry run with Met Ed public relations officials, Behrle goofed. Asked if he thought his grandchildren would be proud of him, Behrle—a bachelor who roars around in his yellow Corvette with his computer operator girlfriend, Mary Lee Garrison, 26—replied: "Well, I doubt if I'll ever have any grandchildren." He laughs: "The PR guy screamed, 'Don't say that! They'll think you're sterile.' All I meant was that since I'm not married I didn't see grandchildren in my immediate future."
For his part, Mike Benson thought so little of the danger that he neglected to tell his wife when the mission was scheduled. "Somebody at work said, 'Tomorrow's Mike's big day,' " says Nancy Benson, 29, a dental hygienist. "I just pretended I knew, but I was a little upset with him when I found out." Mike's major fear, he says, was the repeated physical exams: "The nurses over at the hospital will tell you I'm scared of everything, especially getting a needle in my arm."
After their mission was completed, the two heroes and some colleagues went to a Harrisburg restaurant to celebrate. "We drank a little beer," Benson says. But Behrle recalls the festivities as more traumatic than the accomplishment: "I don't know what Mike had, but I remember that I had two piña coladas, three vodkas with bitter lemon and a bottle of wine," he says. "I'd just as soon you don't ask me to remember the rest of the evening."

redpajama_set_name: RedPajamaCommonCrawl


Liem PH, Morimoto N, Mahara A, Jinno C, Shima K, Ogino S, Sakamoto M, Kakudo N, Inoie M, Kusumoto K, Fujisato T, Suzuki S, Yamaoka T. Preparation of Inactivated Human Skin Using High Hydrostatic Pressurization for Full-Thickness Skin Reconstruction. PLoS One. 2015 Jul 30;10(7):e0133979..
Jinno C, Morimoto N, Mahara A, Liem PH, Sakamoto M, Ogino S, Kakudo N, Inoie M, Fujisato T, Kusumoto K, Suzuki S, Yamaoka T. Inactivation of Human Nevus Tissue Using High Hydrostatic Pressure for Autologous Skin Reconstruction: A Novel Treatment for Giant Congenital Melanocytic Nevi. Tissue Eng Part C Methods. 2015 Aug 21.
Morimoto N, Mahara A, Shima K, Ogawa M, Jinno C, Kakudo N, Kusumoto K, Fujisato T, Suzuki S, Yamaoka T. The rapid inactivation of porcine skin by applying high hydrostatic pressure without damaging the extracellular matrix. Biomed Res Int. 2015;2015:587247. Epub 2015 Mar 24.
Mahara A, Morimoto N, Sakuma T, Fujisato T, Yamaoka T. Complete cell killing by 3 applying high hydrostatic pressure for acellular vascular graft preparation. Biomed Res Int. 2014;2014:379607.Epub 2014 Apr 30.


We'll send high converting traffic straight to your e-commerce store. Get a bespoke marketing campaign that's managed and optimised for sales.
Paid traffic is a key part of a growth-hackers tool kit. Done right it can deliver fantastic ROI, done poorly it can burn a hole in your pocket. So what makes the difference?
It's been said that a site never has a traffic problem, it has a conversion problem. Because we can use Google and Bing to create adverts and get clicks. They key is to get quality traffic that converts. Getting more revenue from the cost of the Adverts.
The good news is with YoGrow everything is tracked, so you'll be able to clearly see your Return on Investment, but how do we achieve it? What tricks do we use?
Well it's quite simple, but it's hard work. We optimise the Adverts and the Audience.
The Adverts are created to be as honest as possible. We only want those who are most suitable to click. So, if you are searching for a very specific item and you stock it, then we'll have a targeted advert that is optimised for that search rather than a generic advert which might not be relevant.
That means creating lots of adverts for lots of different contexts.
Then the audience. This is where we make sure we target the correct people. We'll learn who your customers are and what they search for. We'll then investigate what variations of keywords they use and create attention-grabbing adverts that answer the search question.
We'll send you traffic that converts on ROI. That means we'll look at the average spend of your traffic and optimise your paid search traffic to bring traffic that converts for your price point.
We'll need access to your Google AdWords account, Bing account and your store.
Each Ecommerce platform is different and we can advise on this once we learn more about your store – but the short answer is Yes, both Adwords and Bing are used across many ecommerce stores and integration is not tricky.


The performance and cost advantages of our gallium arsenide (GaAs) based Heterojunction Bipolar Transistor (HBT) technology has enabled several high volume commercial applications. TRW is currently delivering over 4 million MBE based HBT integrated circuits per month for low cost commercial wireless applications, as well as for high performance high reliability defense avionics, ground, and space applications. Indium Phosphide (InP) based HBT technology has several enabling advantages over GaAs HBTs for commercial communication applications, in particular for high efficiency cellular and millimeter wave power amplifiers, 40 GBPS fiber-optic communications, and signal processing ICs.


An underlayer of block printed dots gleam through the top sheer layer of Chanderi in these pants. The bottom cuff is made of chanderi with a subtle gold texture. It has a comfortable straight fit with a gathered waist band. Team this up with any of our foliage dresses/tops for a layered look.
Made in handwoven linen with lace edging.


text:
Sydney Seaplanes goes electric, to convert Cessna in Australia first
Australia's leading seaplane operator Sydney Seaplanes is planning to go electric – signing an agreement with magniX and Dante Aeronautical to convert a Cessna Caravan to service the popular route between Sydney Harbour and nearby Palm Beach.
Sydney Seaplanes plans to extend the electric aircraft service to other routes in NSW and the ACT.
"We are extremely excited to be the first Australian airline to seek regulatory approval to commence fully electric, nil-emissions commercial flights," said Sydney Seaplanes CEO Aaron Shaw.
"Combined with our commitment to use solar technology in our charging facilities, this puts us in a very strong position to become the world's first fully electric nil-emissions airline, subject to the speed of regulatory approvals."
The company will work with US-Australian engine maker magniX and Dante Aeronautical, a developer and integrator of electric and hybrid aviation concepts, to convert the Cessna Caravan into the first all-electric commercial aircraft certified by the Australian Civil Aviation Safety Authority (CASA).
The companies expect to complete ergulatory approvals by the end of the first quarter of 2023, which would immediately allow Sydney Seaplanes the opportunity to start the route from Rose Bay to Palm Beach and offer its certificate program to other Caravan operators in the region.
"That Australia's, and potentially the world's, first fully electric nil-emissions airline will be based at Australia's very first commercial airport at Rose Bay has a beautiful synchronicity to it," Shaw said.
"Electric technology has the potential to completely transform aviation. The environmental benefits are clear, with nil-emissions travel already technically possible on shorter journeys and with the exponential improvement in battery technologies range will continue to increase.
"This will enable us to expand our electric services to other New South Wales and ACT destinations, including Canberra"
MagniX CEO Roei Ganzarski said it is another milestone for the electric aviation revolution.
"Expanding with STC partners globally on more and more aircraft platforms means more sustainable air travel brought to market and to the masses. And for operators, the all-electric aircraft provides the benefits of clean, cost-effective aviation."
This partnership will also serve to solidify the leading position in electric aviation that magniX has secured for itself, following a year after the company flew the world's first commercial all-electric aircraft, a six-passenger DHC-2 de Havilland Beaver.
With engineering facilities in both Redmond, Washington, in the United States, and in Australia, magniX also successfully flew the largest all-electric commercial aircraft, a Cessna 208B Grand Caravan, in Washington earlier this year.
"The iconic Caravan has been a workhorse of industry moving people and transporting goods on short routes for decades," Ganzarski said in May.
"This first flight of the eCaravan is yet another step on the road to operating these middle-mile aircraft at a fraction of the cost, with zero emissions, from and to smaller airports. These electric commercial aircraft will enable the offering of flying services of people and packages in a way previously not possible."
Tesla shares video of 4680 Roadrunner battery line posted on January 18, 2021


text:
using System;
using System.Drawing;
using System.Collections.Generic;
using System.Linq;

namespace SplatterPlots
{
    public interface ISeriesProjection
    {
        System.Drawing.Color Color { get; set; }
        System.Collections.Generic.List<ProjectedPoint> dataPoints { get; }

        bool Enabled { get; set; }
        int[] Histogram { get; set; }
        string Name { get; }
        float Xmax { get; }
        float Xmin { get; }
        float Ymax { get; }
        float Ymin { get; }
    }
    public class SeriesProjection : ISeriesProjection
    {
        private DataSeries m_Data;
        public void Init(DataSeries data, string d0, string d1)
        {
            m_Data = data;
            Enabled = true;
            Name = data.Name;
            m_Dim0 = d0;
            m_Dim1 = d1;

            dataPoints = data.getXYValues(this,m_Dim0, m_Dim1).ToList();
            for (int i = 0; i < dataPoints.Count; i++)
            {
                dataPoints[i].Index = i;
            }
        }
        public bool Enabled { get; set; }
        public int[] Histogram { get; set; }

        public List<ProjectedPoint> dataPoints { get; private set; }
        public Color Color { get { return m_Data.Color; } set { m_Data.Color = value; } }
        public string Name { get; private set; }

        public float Xmax { get { return m_Data.ColumnData[m_Dim0].Max; } }
        public float Ymax { get { return m_Data.ColumnData[m_Dim1].Max; } }
        public float Xmin { get { return m_Data.ColumnData[m_Dim0].Min; } }
        public float Ymin { get { return m_Data.ColumnData[m_Dim1].Min; } }

        public DataSeries Data { get { return m_Data; } }

        public string HorizontalDimName { get { return m_Dim0; } }
        public string VerticalDimName { get { return m_Dim1; } }

        private string m_Dim0;
        private string m_Dim1;
    }
    public class GroupSeriesProjection : ISeriesProjection
    {
        public GroupSeriesProjection(List<SeriesProjection> seriesList, Color color, string name)
        {
            Enabled = true;
            Color = color;
            Name = name;
            dataPoints = new List<ProjectedPoint>();
            int i = 0;

            Xmax = float.MinValue;
            Xmin = float.MaxValue;
            Ymax = float.MinValue;
            Ymin = float.MaxValue;

            foreach (var series in seriesList)
            {
                if (series.Enabled)
                {
                    foreach (var point in series.dataPoints)
                    {
                        point.Index = i++;
                        dataPoints.Add(point);
                    }
                    Xmax = Math.Max(Xmax, series.Xmax);
                    Ymax = Math.Max(Ymax, series.Ymax);
                    Xmin = Math.Min(Xmin, series.Xmin);
                    Ymin = Math.Min(Ymin, series.Ymin);
                }
            }
            Enabled = dataPoints.Count > 0;
        }
        public Color Color
        {
            get; set;
        }

        public List<ProjectedPoint> dataPoints
        {
            get;
            private set;
        }

        public bool Enabled
        {
            get;
            set;
        }

        public int[] Histogram
        {
            get;
            set;
        }

        public string Name
        {
            get;
            private set;
        }

        public float Xmax
        {
            get;
            private set;
        }

        public float Xmin
        {
            get;
            private set;
        }

        public float Ymax
        {
            get;
            private set;
        }

        public float Ymin
        {
            get;
            private set;
        }
    }
}

redpajama_set_name: RedPajamaGithub


Genius Educational HTML5 Template is a modern clean multi-purpose html5 template built with valid HTML5 & CSS3. It's build on top of latest Bootstrap framework 3.3.1 fully responsive web compatible with multi browser and devices. This template can be used for multi-purpose needs like Educational Institutes, colleges, Schools, e-Learning, Training centre, Tutors, Charity, Primary School, business, consultancy, agency, personal portfolio, profile and mobile website.
So We got you the Best Educational HTML5 Template. This is a beautiful modern education website template. This Education HTML Template is well crafted a perfect fit for Colleges, Schools, Institutes, Education, University and many more. Download this Free Education Website Template Now.
This entry was posted in Education, Educational Website, Free HTML5 Templates and tagged bootstrap template, Education template, free website template, html5 responsive template. Bookmark the permalink.


Luxury, luxury, luxury. As the other founding member of The Good Spa Guide, Sybaritic Spy has had more spa experiences in the last few years than most people have in a lifetime. If you want to know about spa, she is the woman to talk to.
"Spa to me is synonymous with sensuality. I want to hear, smell, feel and experience everything. Fragrances to intoxicate, warm treatment beds and unexpected treats make me happy. Black mould, sniffing therapists and people who do not take my lavender allergy seriously do not!"


text:
Nine Year Old Boy Hero
Best College Admission Essay Writing Service Articles and
It was one of the first Spring like mornings in Cleveland for a very long time. Wednesday morning, in March waiting for the bus to come before school two little nine year old boys were shooting hoops in the driveway because they just had too much energy to simply stand and wait. Isn't that typical of all little boys? Go, go, go – "what do you want to do next?" is the eternal question of all active little boys. Except this morning when the kind and compassionate actions of one little nine year old Mickey became a real live hero.
essays to college-paper-writing-service.com/college-essays-for-sale essayshark social example
Nine year old Mickey and his friend Chris were engrossed in their morning game of hoops waiting for the school bus when all of a sudden this black Labrador walked up the drive and plopped down totally exhausted. Mickey started to go up to the dog when Chris shouted, "Don't!" Mickey said, "Why not, I want to help it." "Leave it alone." "No, it needs help." "Common, leave it alone."
reading definition gallego for Premium Essay Help from Reliable Experts on
Mickey walked up to the exhausted dog that just barely had the strength to wag its tail. He stoked its head as it lay in the driveway. At nine years old Mickey had the sense to check out the collar. He noticed a phone number and another series of numbers. He reached in his book bag and on his homework wrote down all the numbers he found.
essays definition northern uc.eap.ucop.edu life
Telling Chris to stay with the dog (as if the dog was going anywhere it was so tired) he hurried into the house not bothering to tell Gramma who babysits the other siblings. He ran to the phone, picked it up and dialed. A woman's soft voice answered, "Thank you for calling the Cleveland Sight Center
how may I help you?" With a strong tiny little voice Mickey said excitedly, "I found a dog and it has numbers on it!" Knowing she was talking to a child the woman asked him to tell her the numbers. She took down the numbers and did a trace as Mickey waited with anticipation.
"Yes!" she said. "She's been reported missing I have their phone number do you want to call them?" "YEAH" said Mickey.
chowan.edu/acadp/science/courses/comparativeanatomy/monotremata/monotrematahtm1.htm transition Articles of essayshark
Mickey was taking down the number when Gramma discovered him in the kitchen. She immediately asked what was he doing inside and why was he on the phone?! While still on the phone, like a speeding bullet Mickey rattled off his story: "we found a dog, she's lying outside with Chris, see, go look, she had a collar, it had numbers on it and I called the numbers, the lady said she's been reported missing and she's going to give me the phone number of the dog's home! She's outside with Chris…her name is Sissy, see, go look and see her, she had numbers on her collar and I called! Can I call them now please, Gramma, huh? Please?"
Totally taken aback and somewhat in shock she asked to speak with the person on the other end of the phone. Mickey handed over the receiver. Gramma listened. Her eyes misted over. She took down the number again and said thank you. She turned to Mickey and said he was a very good boy. Sissy's parents were looking for her. They lived over three miles away. She's come a long, long way and was very lucky Mickey found her. She asked if he would like to call them. And Mickey said ….YES!
Mickey called the Mommy and Daddy of Sissy. They were so happy. Mickey thought he heard the lady crying. They asked if they could come right over to get Sissy. Mickey asked Gramma and Gramma thought there was no better day to be late for school than this day! She said yes. Mickey passed the phone to Gramma and Gramma gave them directions to their house. Mickey flew outside to tell Sissy that Mommy and Daddy are coming to get her. Gramma followed a little bit later and found Sissy's head in Mickey's lap. The bus was coming. Gramma told Chris to get ready for the bus and told Mickey that he can stay and wait with her. "I'll take you to school after they come and get Sissy."
Marge and David came within fifteen minutes. Marge sobbed and sobbed. David cried too. Then they got down on their knees next to Sissy whose head was still in Mickey's lap and told him about Shelly, their daughter. Shelly was seventeen years old and Sissy was her seeing-eye dog. Shelly was blind because she had Retinal Cancer, kind of like cancer in the eyes they told him. She died a week ago and Sissy misses her. Sissy was the only living connection they had to their only child, Shelly.
Mickey was a hero. A nine year old boy who had the good sense to help for no other purpose than….because became a hero. Just because.
They wanted to give him a hero's reward but he refused. Holding on to Grammas hand, he said good-bye to Marge, David and Sissy.
Mickey is my son and I am so very, very proud of him. If I could tell the whole world about him I would… Julie LaRich
Tags: child, childcare, dog, guide dog, hero, humane story, labrador, news


text:
IDH1(R132H) mutation increases murine haematopoietic progenitors and alters epigenetics
Masato Sasaki1 na1,
Christiane B. Knobbe1,2 na1,
Joshua C. Munger3,
Evan F. Lind1,
Dirk Brenner1,
Anne Brüstle1,
Isaac S. Harris1,4,
Roxanne Holmes5,
Andrew Wakeham1,
Jillian Haight1,
Annick You-Ten1,
Wanda Y. Li1,
Stefanie Schalm9,
Shinsan M. Su9,
Carl Virtanen6,
Guido Reifenberger2,
Pamela S. Ohashi1,
Dwayne L. Barber4,
Maria E. Figueroa7,
Ari Melnick8,
Juan-Carlos Zúñiga-Pflücker5 &
Tak W. Mak1,4
Acute myeloid leukaemia
Disease genetics
Haematopoietic stem cells
Mutations in the IDH1 and IDH2 genes encoding isocitrate dehydrogenases are frequently found in human glioblastomas1 and cytogenetically normal acute myeloid leukaemias (AML)2. These alterations are gain-of-function mutations in that they drive the synthesis of the 'oncometabolite' R-2-hydroxyglutarate (2HG)3. It remains unclear how IDH1 and IDH2 mutations modify myeloid cell development and promote leukaemogenesis. Here we report the characterization of conditional knock-in (KI) mice in which the most common IDH1 mutation, IDH1(R132H), is inserted into the endogenous murine Idh1 locus and is expressed in all haematopoietic cells (Vav-KI mice) or specifically in cells of the myeloid lineage (LysM-KI mice). These mutants show increased numbers of early haematopoietic progenitors and develop splenomegaly and anaemia with extramedullary haematopoiesis, suggesting a dysfunctional bone marrow niche. Furthermore, LysM-KI cells have hypermethylated histones and changes to DNA methylation similar to those observed in human IDH1- or IDH2-mutant AML. To our knowledge, our study is the first to describe the generation and characterization of conditional IDH1(R132H)-KI mice, and also the first report to demonstrate the induction of a leukaemic DNA methylation signature in a mouse model. Our report thus sheds light on the mechanistic links between IDH1 mutation and human AML.
Neoantigen-specific TCR-T cell-based immunotherapy for acute myeloid leukemia
Weijun Zhou
, Jinyi Yu
… Kankan Wang
Experimental Hematology & Oncology Open Access 16 November 2022
Exosome-based strategies for diagnosis and therapy of glioma cancer
Mohsen Karami Fath
, Jalil Azami
… Vahideh Tarhriz
Cancer Cell International Open Access 21 August 2022
Defining the landscape of metabolic dysregulations in cancer metastasis
Sara Abdul Kader
, Shaima Dib
… Anna Halama
Clinical & Experimental Metastasis Open Access 18 December 2021
Figure 1: LysM-KI mice show age-dependent splenomegaly and decreased bone marrow cellularity.
Figure 2: LysM-KI mice show age-dependent increases in lineage-restricted progenitors and extramedullary haematopoiesis.
Figure 3: Altered methylation of DNA and histones in LysM-KI cells.
Primary accessions
Gene Expression Omnibus
Data deposits
The microarray and sequencing data have been deposited in the Gene ExpressionOmnibus of the National Center for Biotechnical Information with the accession numbers GSE38589 and GSE38687.
The spelling of an author name (S.M.S.) was corrected
Parsons, D. W. et al. An integrated genomic analysis of human glioblastoma multiforme. Science 321, 1807–1812 (2008)
Mardis, E. R. et al. Recurring mutations found by sequencing an acute myeloid leukemia genome. N. Engl. J. Med. 361, 1058–1066 (2009)
Dang, L. et al. Cancer-associated IDH1 mutations produce 2-hydroxyglutarate. Nature 462, 739–744 (2009)
Xu, W. et al. Oncometabolite 2-hydroxyglutarate is a competitive inhibitor of α-ketoglutarate-dependent dioxygenases. Cancer Cell 19, 17–30 (2011)
Figueroa, M. E. et al. Leukemic IDH1 and IDH2 mutations result in a hypermethylation phenotype, disrupt TET2 function, and impair hematopoietic differentiation. Cancer Cell 18, 553–567 (2010)
Chowdhury, R. et al. The oncometabolite 2-hydroxyglutarate inhibits histone lysine demethylases. EMBO Rep. 12, 463–469 (2011)
Clausen, B. E. et al. Conditional gene targeting in macrophages and granulocytes using LysMcre mice. Transgenic Res. 8, 265–277 (1999)
Ye, M. et al. Hematopoietic stem cells expressing the myeloid lysozyme gene retain long-term, multilineage repopulation potential. Immunity 19, 689–699 (2003)
Goardon, N. et al. Coexistence of LMPP-like and GMP-like leukemia stem cells in acute myeloid leukemia. Cancer Cell 19, 138–152 (2011)
Holmes R & Zúñiga-Pflücker J.-C The OP9–DL1 system: generation of T-lymphocytes from embryonic or hematopoietic stem cells in vitro. Cold Spring Harb. Protoc. http://dx.doi.org/10.1101/pdb.prot5156 (2009)
de Boer, J. et al. Transgenic mice with hematopoietic and lymphoid specific expression of Cre. Eur. J. Immunol. 33, 314–325 (2003)
Ito, K. et al. Reactive oxygen species act through p38 MAPK to limit the lifespan of hematopoietic stem cells. Nature Med. 12, 446–451 (2006)
Tothova, Z. et al. FoxOs are critical mediators of hematopoietic stem cell resistance to physiologic oxidative stress. Cell 128, 325–339 (2007)
Callens, C. et al. Targeting iron homeostasis induces cellular differentiation and synergizes with differentiating agents in acute myeloid leukemia. J. Exp. Med. 207, 731–750 (2010)
Eliasson, P. & Jönsson, J. I. The hematopoietic stem cell niche: low in oxygen but a nice place to be. J. Cell. Physiol. 222, 17–22 (2010)
Zhao, S. et al. Glioma-derived mutations in IDH1 dominantly inhibit IDH1 catalytic activity and induce HIF-1α. Science 324, 261–265 (2009)
Noushmehr, H. et al. Identification of a CpG island methylator phenotype that defines a distinct subgroup of glioma. Cancer Cell 17, 510–522 (2010)
Akalin, A. et al. Base-pair resolution DNA methylation sequencing reveals profoundly divergent epigenetic landscapes in acute myeloid leukemia. PLoS Genet. (in the press)
Campbell, C. et al. Signal control of hematopoietic stem cell fate: Wnt, Notch, and Hedgehog as the usual suspects. Curr. Opin. Hematol. 15, 319–325 (2008)
Heidel, F. H. et al. Self-renewal related signaling in myeloid leukemia stem cells. Int. J. Hematol. 94, 109–117 (2011)
Klinakis, A. et al. A novel tumour-suppressor function for the Notch pathway in myeloid leukaemia. Nature 473, 230–233 (2011)
Söderberg, S. S. et al. Complex and context dependent regulation of hematopoiesis by TGF-β superfamily signaling. Ann. NY Acad. Sci. 1176, 55–69 (2009)
Patel, K. P. et al. Acute myeloid leukemia with IDH1 or IDH2 mutation: frequency and clinicopathologic features. Am. J. Clin. Pathol. 135, 35–45 (2011)
Patnaik, M. M. et al. Differential prognostic effect of IDH1 versus IDH2 mutations in myelodysplastic syndromes: a Mayo Clinic Study of 277 patients. Leukemia 26, 101–105 (2012)
Munger, J. et al. Systems-level metabolic flux profiling identifies fatty acid synthesis as a target for antiviral therapy. Nature Biotechnol. 26, 1179–1186 (2008)
Bajad, S. U. et al. Separation and quantitation of water soluble cellular metabolites by hydrophilic interaction chromatography-tandem mass spectrometry. J. Chromatogr. A 1125, 76–88 (2006)
Meissner, A. et al. Reduced representation bisulfite sequencing for comparative high-resolution DNA methylation analysis. Nucleic Acids Res. 33, 5868–5877 (2005)
Krueger, F. & Andrews, S. R. Bismark: a flexible aligner and methylation caller for Bisulfite-Seq applications. Bioinformatics 27, 1571–1572 (2011)
Fujita, P. A. et al. The UCSC Genome Browser database: update 2011. Nucleic Acids Res. 39, D876–D882 (2011)
Storey, J. D. & Tibshirani, R. Statistical significance for genomewide studies. Proc. Natl Acad. Sci. USA 100, 9440–9445 (2003)
Article ADS MathSciNet CAS Google Scholar
Du, P. et al. lumi: a pipeline for processing Illumina microarray. Bioinformatics 24, 1547–1548 (2008)
Benjamini, Y. & Hochberg, Y. Controlling the false discovery rate: a practical and powerful approach to multiple testing. J. R. Stat. Soc., B 57, 289–300 (1995)
MathSciNet MATH Google Scholar
Smyth G. K Linear models and empirical Bayes methods for assessing differential expression in microarray experiments. Stat. Appl. Genet. Mol. Biol. 3, 3 (2004)
Article ADS MathSciNet Google Scholar
Benjamini, Y. & Yekutieli, D. The control of the false discovery rate in multiple testing under dependency. Ann. Stat. 29, 1165–1188 (2001)
Article MathSciNet Google Scholar
We thank the Animal Research Colony (ARC) at the Ontario Cancer Institute for mouse care; I. Ng, A. Shahinian, J. Sylvester and S. McCracken for administrative and organizational expertise; M. Bailey and J. Tsao for technical assistance; F. Tong and R. Nayyar for assistance with flow cytometric analysis and sorting; the Weill Cornell Medical College (WCMC) Epigenomics Core Facility for technical help and expertise; G. Melino, D. Green, M. Minden, H. Chang and P. Lang for helpful discussions; J. Thomsen for figure layout and M. Saunders for scientific editing. C.B.K. and D.B. were supported in part by a Feodor-Lynen Postdoctoral Research Fellowship from the Alexander-von-Humboldt-Foundation, Germany. D.B. and A.B. were supported in part by a Fellowship from the German Research Foundation (DFG). J.C.M. is supported by a National Institute of Health grant (NIH R01AI081773) and is a Damon Runyon-Rachleff Innovation Awardee supported by the Damon Runyon Cancer Research Foundation (DRR-09-10). P.S.O. holds a Canada Research Chair in Autoimmunity and Tumor Immunity. M.E.F. is supported by the Leukemia & Lymphoma Society Special Fellow Award and a Doris Duke Clinical Scientist Development Award. A.M. is supported by an LLS SCOR grant (7132-08), a Burroughs Wellcome Clinical Translational Scientist Award and a Starr Cancer Consortium grant (I4-A442). J.-C.Z.-P. is supported by a Canada Research Chair in Developmental Immunology. This work was supported by grants from the Canadian Institutes of Health Research (CIHR) and the Ontario Ministry of Health and Long Term Care to T.W.M., and a program grant from the Terry Fox Foundation to P.S.O., J.-C.Z.-P. and T.W.M. Please note that the views expressed do not necessarily reflect those of the OMOHLTC.
Masato Sasaki and Christiane B. Knobbe: These authors contributed equally to this work.
The Campbell Family Institute for Breast Cancer Research, Ontario Cancer Institute, University Health Network, Toronto, Ontario M5G 2C1, Canada,
Masato Sasaki, Christiane B. Knobbe, Evan F. Lind, Dirk Brenner, Anne Brüstle, Isaac S. Harris, Andrew Wakeham, Jillian Haight, Annick You-Ten, Wanda Y. Li, Pamela S. Ohashi & Tak W. Mak
Department of Neuropathology, Heinrich Heine University, 40225 Düsseldorf, Germany,
Christiane B. Knobbe & Guido Reifenberger
School of Medicine and Dentistry, University of Rochester, Rochester, 14642, New York, USA
Joshua C. Munger
Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5G 2C1, Canada,
Isaac S. Harris, Dwayne L. Barber & Tak W. Mak
Department of Immunology, Sunnybrook Research Institute, University of Toronto, Toronto, Ontario M4N 3M5, Canada,
Roxanne Holmes & Juan-Carlos Zúñiga-Pflücker
University Health Network Microarray Center, Toronto, Ontario M5S 1A8, Canada,
Carl Virtanen
Department of Pathology, University of Michigan, Ann Arbor, 48109, Michigan, USA
Maria E. Figueroa
Weill Cornell Medical College, Cornell University, New York, 10021, New York, USA
Ari Melnick
Agios Pharmaceuticals Incorporated, Cambridge, 02139, Massachusetts, USA
Stefanie Schalm & Shinsan M. Su
Masato Sasaki
Christiane B. Knobbe
Evan F. Lind
Dirk Brenner
Anne Brüstle
Isaac S. Harris
Roxanne Holmes
Andrew Wakeham
Jillian Haight
Annick You-Ten
Wanda Y. Li
Stefanie Schalm
Shinsan M. Su
Guido Reifenberger
Pamela S. Ohashi
Dwayne L. Barber
Juan-Carlos Zúñiga-Pflücker
Tak W. Mak
C.B.K., M.S. and T.W.M. initiated the project. M.S. designed the conditional IDH1(R132H)-KI mouse and generated it, as well as the IDH1(R132H)-KI embryonic stem cell clones with the help of S.S., S.M.S., A.W., J.H. and A.Y.-T.; C.B.K. designed and organized experiments involving the hematopoietic system in consultation with T.W.M., M.E.F., A.M., J.-C.Z.-P., G.R., P.S.O., C.V. and D.L.B.; C.B.K. performed the flow cytometry analyses, bone marrow transfers, cell sorting, RNA extraction and real-time PCR analyses, immunoblotting, colony formation assays and serial plating experiments. J.C.M. performed the LC-MS analyses. E.F.L., D.B., A.B. and I.S.H. helped with flow cytometric analyses and BM transfers. R.H. performed the OP9/OP9-DL1 embryonic stem cell in vitro differentiation experiments. W.Y.L. helped with flow cytometric analyses and cell culture experiments. M.E.F. and A.M. performed the DNA methylation experiments and analysed the data. C.V. performed the mRNA expression microarray experiments and analysed data. C.B.K. and T.W.M. wrote the manuscript with the help of A.B. and D.B. All authors discussed the results extensively and agree with the conclusions presented in the manuscript.
Correspondence to Tak W. Mak.
The authors declare no competing financial interests.
This file contains Supplementary Figures 1-11, Supplementary Tables 1-2 and 6-7 and legends for Supplementary Tables 3, 4 and 5 (see separate files for these tables). (PDF 1609 kb)
Supplementary Table 3
This table contains differentially expressed mRNAs in LysM-KI and control LSK cells - see Supplementary Information file for full legend. (XLS 77 kb)
This table contains Gene Ontology categories of the differentially expressed mRNAs - see Supplementary Information file for full legend. (XLS 836 kb)
This table contains differentially methylated genomic regions - see Supplementary Information file for full legend. (XLS 219 kb)
Sasaki, M., Knobbe, C., Munger, J. et al. IDH1(R132H) mutation increases murine haematopoietic progenitors and alters epigenetics. Nature 488, 656–659 (2012). https://doi.org/10.1038/nature11323
Issue Date: 30 August 2012
Jinyi Yu
Kankan Wang
Experimental Hematology & Oncology (2022)
Jalil Azami
Vahideh Tarhriz
Oncogenesis induced by combined Phf6 and Idh2 mutations through increased oncometabolites and impaired DNA repair
Tsung-Chih Chen
Chi-Yuan Yao
Hwei-Fang Tien
Oncogene (2022)
Wild-type isocitrate dehydrogenase under the spotlight in glioblastoma
Gabriel Alzial
Ophelie Renoult
Claire Pecqueur
Focal disruption of DNA methylation dynamics at enhancers in IDH-mutant AML cells
Elisabeth R. Wilson
Nichole M. Helton
David H. Spencer
Leukemia (2022)
An IDH1–mutant-mouse leukaemia model
Mutations in the IDH1 and IDH2 genes, which encode isocitrate dehydrogenases, are frequently found in human glioblastomas and acute myeloid leukaemias. These mutations drive the synthesis of the metabolite R-2-hydroxyglutarate (2HG), which inhibits enzymes that regulate levels of DNA and histone methylation. Here, Tak Mak and colleagues characterize conditional knock-in mice of the most common IDH1 mutation, IDH1–R132H, expressed in haematopoietic cells. The mutant mice have increased numbers of early haematopoietic progenitors and develop splenomegaly, anaemia and extramedullary haematopoiesis. Furthermore, cells exhibit changes in patterns of DNA and histone methylation that are similar to those observed in human IDH1/2-mutant acute myeloid leukaemias. This mouse model should be useful for the study of mechanistic links between mutant IDH1 and leukaemia.


text:
Note: The below presumes you have Docker and Docker Compose installed. You can [find an installer for your OS here](https://docs.docker.com/compose/install/).

To run a stack with the frontend, backend, and Zipkin server, simply run the following: `docker-compose up`

This will create the following endpoints:

1. Zipkin: http://localhost:3000
2. Frontend: http://localhost:8081
3. Backend: http://localhost:8082


Probation list - EARTHSIDE HOLISTIC, LLC.
for unacceptable actions against a client or family's well-being.
Earthside Holistic trains their Doula's to hold the highest form of respect and accountability to their clients. Our Doula's sign a contractual agreement to adhere to Earthside Holistic's Code of Ethics in order to hold our certification. In signing, they agree they will do no harm and agree to be added to this list should they commit ethical violations. Should any Doula be found to be in violation of these Codes, Earthside reserves the right to revoke certification and add their name, general offense and accountability actions to this probation list. We do this in effort to keep our clients and community informed and safe from those who would harm them. Earthside offers a 6 month probationary period to any Doula who has violated the Code of Ethics as well as offer additional training and mentorship, because we believe in second chances (especially for unintentional offenses). However, Earthside will not issue or reinstate certification to repeat offenders.
This is our Code of Ethics that every EEOLD agree's to on their first day of class.
I agree that as an End of Life Doula, I will conduct my behavior in a manner that will honor this growing profession, the client and family, and the end of life process.
I agree that as an End of Life Doula, I will uphold the highest level of personal and professional integrity and implement the standard of care with truth and accountability. Further, I agree to consistently remain reliable and dependable to my clients.
I agree that my responsibilities as an End of Life Doula lie first with my clients. I agree to uphold the rights and choices of my clients within the boundaries of state and federal laws.
I agree that as an End of Life Doula, I will respect the State and Federal mandated privacy acts. Further, I will take applicable precautions when posting anything on the internet.
I agree that as an End of Life Doula, I will maintain respectful interaction with all end of life professionals. ie: Doctors, Nurses, Social Workers etc.
I agree that as an End of Life Doula, I will endorse and encourage the growth and expansion of the End of Life Doula profession and promote End of Life Doula certification to potential interested participants.
I agree that as an End of Life Doula, I will honor the needs to care for myself physically, mentally, emotionally and spiritually, as needed, to maintain health and strength as it pertains to my own life.
I agree as an End of Life Doula, I will remain current and competent in my skills and abilities and to enhance the professional development of End of Life Doula's in Utah through my certification. Further, I will attend the annual education session and seek out more opportunities for Continuing Education.
I agree as an End of Life Doula to support, encourage and mentor my fellow End of Life Doula's.
I agree to adhere to this Code of Ethics set forth by the Earthside End of Life Doula Training Program, and to hold Earthside Holistic and its instructors/owners/affiliates harmless in the event of unforeseen complications or erroneous behaviors on my part. I agree that I willfully participated in the Earthside End of Life Doula Certification Program and understand that my instructors have given me the best local training available.