There is a certain sense of comfort from knowing you have made the reservations for the night's dinner or you have triple checked the weather before the family picnic.
The epitome of the good student, likely to succeed in life and the ideal candidate for a promotion, is one who organizes their time and plans for it all.
The endless to do list seems a bit more manageable when every activity has been squeezed into its own time. Daily planners, weekly planners, monthly planners. Big squares, small squares, lined squares, blank squares. At this age, the digital planner.
With Thanksgiving break around the corner I should feel excited for a restful respite from hectic school days. Yet, I only feel dread thinking about the overwhelming number of appointments and assignments that I must finish.
Even vacations, meant for relaxation, have schedules set for hotel check ins, rental car pick ups, massage times, restaurant times and return flight times.
In the 21st century, we are always working with an insatiable desire for a better phone, a better job, a better life. We plan daily so we can work endlessly and reach the greener grass.
Peel ourselves away from the planner and for one day go out there and let life happen.
Do not make reservations. Take the family out to a fun fast food. Better yet, cook at home. Have your picnic in the rain: maybe the picnic basket cannot be opened, but suddenly, your family opens up.
The most valuable characteristic that a student or professional should have is handling the unexpected, taking things in stride and making the best of every situation.
According to businessman Federico Foli, we should all learn to let go of control.
Although we don't have any scheduled vacations this Thanksgiving, perhaps my family will take an unanticipated trip. If we do, we will be sure to explore the new city without looking down at guidebooks and maps, but into the faces of local residents. After all, they know the best foods, the coolest attractions and most beautiful spots of the city.
Take a break from the check engine lights (at least temporarily), missed appointments or stressful coworkers. Meet life head on: run in a new park, eat at a street vendor and most importantly – do not check the time.
Suddenly, the grass around you is greener.
I love this! As a planner person myself, I think it really is important to take a step back and let things play themselves out.


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Book Discussion on Choosing the Hero : CSPAN2 : August 6, 2016 4:00pm-4:46pm EDT
banking for development positions with world bank, citibank, hsbc and united nations, returning to liberia she ran for a senate seat but after speaking out against the military regime she landed in jail, back once more a decade later she ran unsuccessfully under warlord charles taylor and again went into exile with transition to democracy in the general election in 2005, she ran a second time for president and won in a runoff. taking charge of a nation shattered by years of civil strife, grappling with burdens of widespread poverty. she was reelected in 2011, the same year she shared the nobel peace prize with two other courageous women are recognized for their, quote, nonviolent struggle, the safety of women and women's rights to full participation and peacebuilding. her story is told in a new book, choosing -- "choosing the hero: my improbably journey and the rise of africa's first woman president" who was a featured guest this afternoon, k. riva levinson is actually here at the moment. as head of the national communications and government relations firm k. riva levinson represen
banking for development positions with world bank, citibank, hsbc and united nations, returning to liberia she ran for a senate seat but after speaking out against the military regime she landed in jail, back once more a decade later she ran unsuccessfully under warlord charles taylor and again went into exile with transition to democracy in the general election in 2005, she ran a second time for president and won in a runoff. taking charge of a nation shattered by years of civil strife,...
Democracy Now! : KCSM : August 30, 2016 12:00pm-1:01pm PDT
, hsbc, ubs, goldman sachs, wells fargo, jp morgan chase, and other financial institutions have combined extended a 3.75 billion credit line to energy transfer, the parent company of dakota access llc. meanwhile, protests are also continuing against oil and gas pipelines in the eastern united states. on monday, two activists were arrested in west roxbury, massachusetts, after they locked themselves to cars to protest the construction of the spectra energy gas pipeline. in new york city, eight people were arrested blockading the doors of national grid, to protest the electric company's support for the spectra energy's aim pipeline, which is slated to run only hundreds of feet from the aging indian point nuclear power plant. this is alexandra zevin speaking to democracy now! as she was getting arrested. >> why should new york state residents take all the risk in the corporation go off with all of the money? it is ridiculous. we need solar energy. we need when the power. amy: the growing protests against oil and gas pipelines come as nasa's top climate scientist, gavin schmidt, is
, hsbc, ubs, goldman sachs, wells fargo, jp morgan chase, and other financial institutions have combined extended a 3.75 billion credit line to energy transfer, the parent company of dakota access llc. meanwhile, protests are also continuing against oil and gas pipelines in the eastern united states. on monday, two activists were arrested in west roxbury, massachusetts, after they locked themselves to cars to protest the construction of the spectra energy gas pipeline. in new york city, eight...
Democracy Now! : KCSM : August 9, 2016 12:00pm-1:01pm PDT
companies. it is like the hsbc deal when they walked away with a relatively small fine for laundering hundreds of million dollars of dollars for g cartels. i think it is shameful. amy: this is what infuriates so many and why so many have come out against the establishment, what is donald trump or bernie sanders. matt: right. amy: how do you see this playing out as you have watched trump's surge and now being roundly condemned? the latest, 50 national security experts, officials coming out against him. they are republican. and then what hillary clinton represents. matt: i remember talking just after the crash, i guess it was almost eight years ago now, a lot of people on wall street and therehington felt that if were not adequate measures to clean up the financial services sector after what happened, there was going to be some kind of a social movement, whether it was on the left or right. people were going to be so upset that somewhere, this was going to impact, create some unrest somewhere. i think that movement really coalesced on the right and the republican side, and they overthre
companies. it is like the hsbc deal when they walked away with a relatively small fine for laundering hundreds of million dollars of dollars for g cartels. i think it is shameful. amy: this is what infuriates so many and why so many have come out against the establishment, what is donald trump or bernie sanders. matt: right. amy: how do you see this playing out as you have watched trump's surge and now being roundly condemned? the latest, 50 national security experts, officials coming out...
France 24 : LINKTV : August 3, 2016 2:30pm-3:01pm PDT
the brexit vote. in at 3.61its came billion dollars, missing forecasts. last year hsbc announced a plan to slash jobs and shift focus to asia. taiwan,ted out of misrepresented themselves as an i.t. platform. market in 2013e angering domestic taxi drivers. time warner has picked up a to boost thehulu platform's visibility as to as anons itself alternative to cable television. networks, including cnn and cartoon network, will be part of hulu's online tv service. two days to go until the olympics kick off in brazil and game's merchandise has been flying off shelves. organizersearner for and retailers who have permission to make t-shirts and the olympic logo. authorities in brazil, however, have been cracking down on the products.unterfeit s see an: brazilian olympic sized business. this hanger of official of 1800ise spread out meters houses everything t-shirtsrelated from to sandals, over 3,000 products with a stream of eager customers them. 20,000 people are expected to pass through here every day. >> you have to really go for it when you're inside. >> it's really cool to wear the ol
the brexit vote. in at 3.61its came billion dollars, missing forecasts. last year hsbc announced a plan to slash jobs and shift focus to asia. taiwan,ted out of misrepresented themselves as an i.t. platform. market in 2013e angering domestic taxi drivers. time warner has picked up a to boost thehulu platform's visibility as to as anons itself alternative to cable television. networks, including cnn and cartoon network, will be part of hulu's online tv service. two days to go until the olympics...
France 24 : LINKTV : August 3, 2016 5:30am-6:01am PDT
seen gains early on, but we have seen the markets turned to the red in the past while. hsbc is up by nearly 4% in london. it is not such a great day for droppedo -- shares have nearly 0.5% after a 50% slump in profits in the first half of the year. anchor: next, we will go to india, where the parliament is discussing major economic reforms. >> it could be the biggest change to taxes in india since independence. they will vote on a national goods and services tax. the tax is a central part of prime minister narendra modi's efforts to overhaul the indian economy. let's join our correspondent in new delhi. how important is this reform for the country? off, this bill has been in limbo for a decade now. it was first introduced to the indian parliament in 2006. as you mentioned, once it is implemented, it will be the biggest tax reform in independent india. it would impose a national sales tax that would create one of the biggest single markets in the world. it would replace 17 existing state and federal taxes, making the movement of goods cheaper and more seamless across the market with 1
seen gains early on, but we have seen the markets turned to the red in the past while. hsbc is up by nearly 4% in london. it is not such a great day for droppedo -- shares have nearly 0.5% after a 50% slump in profits in the first half of the year. anchor: next, we will go to india, where the parliament is discussing major economic reforms. >> it could be the biggest change to taxes in india since independence. they will vote on a national goods and services tax. the tax is a central...
KTVU FOX 2 News at Noon : KTVU : August 9, 2016 12:00pm-1:01pm PDT
the robberies. the first one happened at the hsbc bank in cupertino. each time the robber showed a gun and anded a note to a bank teller. during the robbery in sunnyvale though, witnesses saw the robber touch a pamphlet and reported that to police. investigators say that was the break they needed. >> this is a person who is not going to stop committing robberies. it may have been only a matter of time that somebody could have been hurt. >> fingerprints on that pamphlet match prints on file for a woman who had been arrested for stealing a car. police arrested 27-year-old mary maple at her home in east palo alto. they found a gun, a wig, and clothing that matched what the robber was wearing in surveillance photos. >>> san leandro police are searching for a missing teenage girl who was last seen saturday night. take a look at the picture the 15-year-old deanna benedetto. her mother says her disappearance is abnormal. she is 5' 5", weighs 200 pounds. anyone with information is asked to call san leandro police. >>> homicide investigators searching for the killer of a 20-year-old college
the robberies. the first one happened at the hsbc bank in cupertino. each time the robber showed a gun and anded a note to a bank teller. during the robbery in sunnyvale though, witnesses saw the robber touch a pamphlet and reported that to police. investigators say that was the break they needed. >> this is a person who is not going to stop committing robberies. it may have been only a matter of time that somebody could have been hurt. >> fingerprints on that pamphlet match prints...
KTVU Mornings on 2 at 4:30am : KTVU : August 9, 2016 4:30am-5:01am PDT
taken during one of those robberies the person happened at the hsbc bank cooper back on june 3. now, each time that the robber showed a gun and handed a gun to -- a note to a bank teller. they got the big break during a robbery were witnesses said that the robert touched several items including a pamphlet near a teller's window. investigators picked up fingerprints from the paper. >> normally, you don't get good quality prints in situations like this. so, to get one print and have it come back and match the suspect and then, be able to do a search warrant and get everything to those current -- to tie him to those crimes, it is great. >> she had been arrested for stealing a car, they arrested her at her home in east palo alto at the house they also found a gun, a wig, including that match what a robber -- the robber was wearing. she has been charged with four robbery feis for robberies so far. -- for robberies so far. >>> investigators are searching for the killer of the 20-year- old college student that is getting national attention. a graduate of the high school in san mateo it was
taken during one of those robberies the person happened at the hsbc bank cooper back on june 3. now, each time that the robber showed a gun and handed a gun to -- a note to a bank teller. they got the big break during a robbery were witnesses said that the robert touched several items including a pamphlet near a teller's window. investigators picked up fingerprints from the paper. >> normally, you don't get good quality prints in situations like this. so, to get one print and have it...

redpajama_set_name: RedPajamaCommonCrawl


Kirkwood and Iowa Streets in Davenport, Iowa.
Click here to view a map of the location of the church.
so that all may participate.
the third floor of the Christian Education Building.
at 12:30 pm on Fridays.


Soybean prices rallied and closed higher today, breaking above the $10.00 resistance mark to finish at $10.07 ¾ in the March futures contract. The 17 ¼ cent move for the day was possibly in response to the Commitments of Traders report this past Friday, which showed that speculators had built up near-record net short positions, and led to a general buy-back of contracts today.
Corn managed to finish higher by 2 ¼ cents today after trading lower for much of the day, and closed at 3.89 ½ in the March.
Export inspections for both corn and beans this week were in line with expectations, and the NOPA bean crush report released today pegged January crush right in line with expectations, at 162.7 mbu (a record for January, but below December levels).


text:
Transcript of remarks by CE and CS
Following is the transcript of remarks by the Chief Executive, Mr C Y Leung, at a media session on the progressive abolition of the "offsetting" arrangement under the Mandatory Provident Fund (MPF) System today (June 23):
Reporter: Could you please just describe the current plan that you're rolling out and why is it that the Government feels there's a need to intervene like the Chief Secretary just said? And are you giving workers false hope here, because, as you mentioned, the new Government can overturn this if they wish to?
Chief Executive: The work to abolish the "offsetting" mechanism actually started a long time ago, and there was a working group led by the then Chief Secretary and joined by the then Secretary for Labour and Welfare, have been working on this and have been listening to the views of stakeholders, including employers and employees. And that led to a statement in my Policy Address in January this year, and after that we continued the work and finally the paper reached the Executive Council. And while the Executive Council paper was being prepared, one obvious observation was that both the employee side and employer side became more focused on this issue and they have come up with counter-proposals and proposed amendments and so on, so forth. And so it isn't something that we started a couple of weeks ago. It's something that the employee side has been asking for a long time. So the timing is this. And the incoming Government, as all incoming Governments do, have a prerogative to do whatever the incoming Government think is right regarding this abolition of "offsetting" mechanism proposal or, for that matter, any other proposal that we made.
Reporter: ... stick to what you have proposed now, because, as you said, you know, this concerns ...?
Chief Executive: It is not for me to advise. I'm sure the incoming Government will have its view. It may be the same as ours, may not be.
Ends/Friday, June 23, 2017


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Home Accupuncture , Diet , Donor Egg , Fertility , Herbs , IVF Beat the Biological Clock
Beat the Biological Clock
Catherine McDiarmid-Watt | Saturday, December 27, 2008 | 0 comments
It's the equivalent of daylight saving time for the biological clock, more and more women are rolling it back to get pregnant.
Herbal remedies, acupuncture, and in-vitro fertilization are some of the treatments some women try to have a baby beyond age 40.
A treasure map to a baby? That's just one of many things Meredith Kolk tried. At 40, she longed for another child but knew the odds were against her.
"You hear about so many statistics that fertility really goes down at 40," she says.
Reporter Alina Cho asks, "Did that freak you out?" "It did. It was very scary," she responds.
So Meredith turned to invitro fertilization, hoping to increase her odds. She also used alternative approaches such as meditation, acupuncture, changes in her diet and that treasure map that kept her focused on her goal.
Alina asks, "There are those people who think this is a little nuts, a little crazy?
"They might but the process itself makes you feel a little crazy," Meredith says.
What Meredith is going through is increasingly common as more women 40 and older try to get pregnant.
"When I first got into the field, women over 40 weren't even encouraged to have treatment because they weren't going to get pregnant," Dr. Alice Domar says.
However, she says times have changed. She runs a mind-body center in Boston that specializes in women struggling to get pregnant. Her research shows a medical link between stress and infertility.
"If you can teach women stress management and relaxation techniques their pregnancy rates on average double," she explains.
On this day, 39-year-old Sarah Weatherhead undergoes acupuncture. She believes being more relaxed could help her chances of getting pregnant. She's had IVF three times without success and is awaiting results of her fourth invitro-fertilization.
"I attempt to be very positive about it, but it's difficult, especially when I've had so many failed cycles," Sarah says.
Sarah's playing the ultimate game of beat the clock. In fact, one study shows 33 percent of women infertile by age 40, increasing to 87 percent by age 45. Doctors say the best hope for older women is donor eggs.
"A lot of women initially are very reluctant to go down the route of donor egg because they'll lose that genetic connection with the child. But the fastest way to get a child into your home is usually with donor egg," Domar says.
So successful, a few weeks ago, a 70-year-old woman in India gave birth. While no age limits are enforced in the U.S., most fertility centers maintain a cutoff between 50 and 55.
Alina asks, "What do you think you can offer now that you maybe couldn't offer as a 25-year-old mother?" "I think I'm smarter. I like myself better," Sarah says.
Sarah is still hoping and as for Meredith? At age 41, her family has just increased by two.
"When you were making that manifestation board, is this exactly what you pictured and hoped for?" Alina asks Meredith.
"There was one baby in the original but we were lucky enough to have two, but yes this is," Meredith answers.
Source: http://www.todaysthv.com/news/local/story.aspx?storyid=77656&catid=2
50 Things You Can Do Today to Increase Your Fertility
by Sally Lewis and Nim Barnes
-- Practical advice and a holistic approach to help you conceive, including simple dietary and lifestyle changes and do it yourself complementary therapies.
In this accessible and informative guide, Sally Lewis explains how age, sexual infections, diet, excess weight, stress, and anxiety affect fertility.
Teaching how to discover the best time for conception; understand the link between body, mind, and fertility; and manage stress and relax to prevent anxiety, this is the ultimate guide to increasing the likelihood of conception at any age.
Click to order/for more info: 50 Things You Can Do Today to Increase Your Fertility - US | CDN | UK
Start reading 50 Things You Can Do Today to Increase Your Fertility on your Kindle in under a minute!
Category: Accupuncture, Diet, Donor Egg, Fertility, Herbs, IVF


Erica Hinson Denny inspired many of us to be better while she was here and I'm certain she will continue to for many years to come.
For examples of Erica's photography and more follow this link.
While most of us are aware of Erica's stunning photography, it was not her only talent. For more examples of her art follow this link.
I've heard people call Baltimore "Charm City," but for me, it's not just charm, it's love. If acceptance, giving, sharing, laughing, and creating are essential to love, there has never been a better example of that love than you Erica Hinson Denny.
Your love and art touched everyone. Your amazing talent and indomitable spirit was contagious- like a laugh that starts another laugh until the whole room is rolling on the floor- it was always obvious you were there. It just doesn't seem possible or right that your good energy and love could be gone so soon.
I remember the first time I saw you in a club. It was like watching a miner with a lamp. You were bringing the light of the sun to a dark place. It was also like watching a magician; I didn't know what was about to happen. Flash, flash, flash you were all around the person or people you were photographing; popping and bobbing with intensity. Then you would stop and immediately share the moment using the monitor on the back of the camera. The photograph was an incredible combination of the scene usually in multiple exposures. I watched as even the most weary and jaded-looking clubber would light up or shake their head in disbelief as they looked at the incredible image you had just created. I was amazed by your energy. I hadn't seen your work yet but I knew from that kind of life-force it had to be good.
When we finally met that night you told me that you felt you knew me, you were sure that you knew me. I felt the same way. Looking back I know that you just saw in me, what i saw in you and that's warmth and acceptance.
I want to thank you for your ability to share and openly give what you have. In a world of competition and pettiness you had the courage to share your love and your gifts freely with me and everyone you knew. I know as a stubborn rebel you'd cringe at this, but Erica you set a good example.
As reality sets in I miss you more and more, but I'm grateful that I met you and I have your example. You have helped me become a better artist, friend and man.
Photo and letter by Philip Laubner.
We'd like to recognize Erica's friend and mentor, Jack Radcliffe.
"RIP Erica Hinson Denny ("Artfisch")" by Tom Swiss.
*The following photos taken by Erica were contributed to What Weekly by Erica Hinson Denny and previously published on May 19, 2010.
Believe, will I seem naive?
"Go not yet away from us. A noontide have you been in our twilight, and your youth has given us dreams to dream. No stranger are you among us, nor a guest, but our [light] and our dearly beloved. Suffer not yet our eyes to hunger for your face." — Gibran.
Erica Hinson Denny struggled with chronic asthma for her entire life. In the end she finally succumbed. We've been asked to provide this link to the Asthma and Allergy Foundation of America. There you can leave a donation and learn more.


management, science, policy, and public-private partnerships.
CONTACT: Mr. Thomas M. Holm, Chief, Data Services Branch, U.S.
at (605) 594-6142 or email at holm@usgs.gov.
Remote Sensing Data Archive Advisory Committee are open to the public.
available for public review at http://frwebgate.access.gpo.gov/cgi-bin/leaving.cgi?from=leavingFR.html&log=linklog&to=http://edc.usgs.gov/programs/nslrsda/advcomm.html.


Make the rest of your life the best of your life. Read it daily for the maximum positive impact to your subconscious mind. Who knew Designer Fire Extinguishers could be so therapeutic!
Safe-T is a chic, stylish and uniquely designed fire extinguisher. Attractive and elegantly designed, let GOOD LIFE spice up any interior and keep you safe.


text:
Powered by self-loan, Mike Durant takes fundraising lead in Alabama Senate race
Updated: Apr. 18, 2022, 4:17 p.m. |
Published: Apr. 18, 2022, 12:12 p.m.
Republican Senate candidate Mike Durant addresses the Republican Men's Club in Huntsville on Jan. 15, 2022. (Paul Gattis | pgattis@al.com)
Paul Gattis | pgattis@al.com
On the strength of another loan to his campaign, Mike Durant has taken the fundraising lead in Alabama's Republican Senate primary.
Two days before the reporting period ended on March 31, Durant loaned his campaign $2.65 million to push ahead of Katie Britt for the most money raised since entering the race.
Excluding that loan, however, Britt raised more than four times as much money as Durant for the most recent reporting period, which covers Jan. 1 to March 31.
Related: Katie Britt, Mo Brooks agree to Senate debate, urge Mike Durant to participate
Related: Alabama Senate candidates trade shots over immigration, but all three want the wall
Altogether, Durant – a Huntsville business executive and former Army aviator and prisoner of war -- has loaned his campaign $6.8 million, according to Federal Election Commission campaign finance reports. Neither opponents Britt, former president and CEO of the Business Council of Alabama, nor U.S. Rep. Mo Brooks have loaned any money to their campaigns. The personal money Durant has poured into his campaign surpasses the total contributions raised by Britt and Brooks.
As of the end of March, Durant has $4.096 million in cash on hand while Britt has $3.228 million and Brooks has $1.570 million.
In the most recent reporting period, Britt brought in $1.394 million over the past three months while Durant raised $291,846. Brooks also topped Durant's fundraising, taking in $489,675.
In a statement Monday, the Britt campaign said 84% of the contributions she has received have come from donors in Alabama.
The Britt campaign also criticized Durant for accepting $49,300 during the first quarter of 2022 from America's Promise, a super PAC that has ties to More Perfect Union – another super PAC that is working to elect moderate Senators, according to a report by Fox News. Jake Harriman, the founder of More Perfect Union, is listed as the treasurer of America's Promise.
America's Promise also donated $1 million in December to Alabama Patriots PAC, which is supporting Durant.
"My family and I are honored and grateful that so many hardworking Alabamians are joining our conservative grassroots campaign," Britt said in a statement. "The momentum we're seeing every day on the ground makes it clear that families across our state know that I will fight to put America and Alabama first, defend our Christian conservative values and preserve the American Dream for our children and our children's children. It's pretty obvious now why Mike Durant is afraid to debate – the truth is out about who is behind his campaign and what he really believes behind closed doors."
The Durant campaign did not immediately respond to a request for comment.
As far as spending, Durant and Britt are in a dead heat. Durant has spent $3.164 million so far while Britt has spent $3.151 million. Brooks has spent $2.285 million.
Britt has been the frontrunner in fundraising since entering the race in June 2021 until this latest report and Durant's self-loan. Through the previous report ending Dec. 31, Britt had raised $4.998 million while Durant had raised $166,153 excluding his $6.8 million loan. Brooks had raised $2.191 million.
Updated today, April 18, 2022, at 4:16 p.m. to clarify the background of America's Promise.


If you are planning or have already booked a trip to Indonesia, and are visiting the islands of Bawah and Bali, you may be unsure what you need to pack. This blog post aims to take the mystery out of that.
Bali is a great place to go on holiday, any time of the year. Thanks to the fact it is warm all year round with a tropical climate, packing for a trip here, in terms of clothes is not actually as difficult as you may think.
We know that packing in general can be something of a stressor for most people, but we are here to help. If you've been before, you will have a better idea, but if this is your first time, you've come to the right place as we are going to impart some handy tips and advice for packing for your trip so that you get the most out of your Indonesian experience.
The smaller bag option also has the bonus that you may be able to avoid waiting for your luggage at the airport at either side of your trip. If it meets the regulations you may be able to take it on to the plane with you.
Obviously, the amount of clothing you take depends on how long your trips is. With regards to things like underwear and other items you are likely to change, you will need a pair for every day, but you may be able to get away with having less of other items that could be worn more than once, like skirts and trousers or shorts.
Remember, you will only be packing lighter pieces of clothing like t-shirts, short-sleeve shirts, vests and although you need to cover up for some of the areas in Bali, this can be done easily by wearing a scarf or sarong if you're a woman. Temples are places where you are expected to show respect for the customs and fact that they are places of religious significance.
For a beach-based holiday, you need to bring beach towels, right? Wrong. Most hotels and other resorts that you are likely to stay at will normally provide you with beach towels. So, check with wherever you are staying to see if they do. If not, you can still save space by just buying some breach towels once you arrive.
They are relatively cheap and your shoulders and hands, as well as your bank balance will benefit from only adding them to your bag once you have arrived.
As Bali is a perennially hot place, you will find when you are not exploring the interesting architecture, temples and marketplaces that you spend a lot of time at the pool or beach. Therefore, swimming costumes, trunks and bikinis are essential items you need to have in your bag. The great thing about these items is that they do not take up much space.
While you are there, you will probably want to carry your money and day to day belongings in a bag, so it's safe and secure and frees up your hands. The Wombat Leather Urban Satchel is a great choice for this kind of everyday bag. Not only does it fit nicely and comfortably over your shoulder, it has plenty of room for all your essentials without being too heavy or cumbersome.
It helps that it also looks stylish too.
Hats are more than just stylish accessories, especially when you are visiting the islands of Bawah and Bali, off the coast of Indonesia. While you will want to bask in that sun and benefit fully from it when you are at the beach or at the side of the pool, there are other times when you'll want to protect your head, face and eyes from the bright and blazing sunshine.
This is why we would always advise that you invest in a good quality leather hat. Two great options in this regard are Wombat Leather the Wilds Foldable Leather Hat and Wombat Soft Distressed Brown Foldable Leather Hat.
You will definitely look trendy and cool walking through the towns and markets and they are not too over the top so that you will look like a tourist.
Both options are made from high quality leather and as well as being incredibly robust and durable, they also benefit from being very practical. How so? They can be folded away when you are not using them. Hats can be a little cumbersome, which is why Wombat Leather have ensured these can be folded right down and will not take up too much space either in your bag or pocket.
When you are on holiday, especially in picturesque locations like Bali and Bawah islands, you want to document your adventures. However, you can make the mistake of taking too much technology.
Put it this way, the more technology you take with you, means the more insurance you may have to invest in. It also means that more space in your bags is going to be taken up not only by the gadgets and gizmos themselves, but things like chargers and other accessories you may need.
It's much wiser then, to keep your baggage as lightweight as possible, to pack only the tech you actually need. Certainly, take a camera and your smartphone, but do you really need your laptop, tablet and smart watch.
You may even be able to skip taking the camera if you have a decent enough snapper built into your smartphone.
Another thing to remember with regards to technology on holiday. The more stuff you bring, the more stuff you have that can go missing, break or even worse, get stolen.
So, we say save those woes and stresses and try to limit the amount of technology you take to the items you really need. Everything else can stay at home where it is safe and secure.
While we totally understand the stress surrounding packing for a trip to Bali. Particularly if you are going from the UK. It is, after all, the other side of the world. However, with the right amount of forethought and planning, it is relatively simple. We hope you can see that from our guide above, which although it is not exhaustive, it does contain a lot of the essential building blocks you need to pack sensibly for your trip to Bali and Bawah. A holiday should be enjoyed and holidays start, we feel, from the moment you leave your house to go to the airport. Therefore, take the misery out of packing so that you can reserve your energy to get the most out of your trip as possible.


Hire a professional company for lawn maintenance: Forget about doing things on your own, unless you have a lot of time to experiment new things. If you want to get professional work done for your yard or lawn, hire a professional company for your lawn maintenance needs. Get amazing work done on your lawn and let your neighbors will go GAGA over your awesome lawn.
Have a swimming pool constructed for your kids: Swimming pool always fascinates the little kids; let them have their very own pool in the outdoor so that they don't tell you to take them to the beach during their vacation.
Make sure the lawn service company transforms your dull lawn into a beautiful piece of nature that has a lot of flowers to give that perfect look to your outdoor area: Lawn maintenance companies know how to change the entire look of your lawn; let them do their job outside, as you sip coffee inside the house.
Get that perfect fairy tale look for your lawn: There are a lot of lawn maintenance companies that provide you with lawn transformation services as well. Talk to a professional company and let the team know what you are looking for; if the team is experienced, it would surely give you the best fairy tale look for your lawn.
Buy an outdoor chess for your loved ones: This may sound expensive to you, but it's not. Besides, this is the best way to create and develop an interest for chess in your kids. Let them play chess with large pieces placed in the lawn of the house.
Pink and Green Lawn Care and Landscape company is a lawn care maintenance company based in Broward County, Florida. We provide lawn related services like mowing, landscaping, landscape design and installation. We also cater to areas of pest control, fertilization, trimming and much more. We have been awarded with Angie's List Super Service Award consecutively for three years. Pink and Green has received A+ ratings from Google as well as Angie's List.


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Anthony Etchells
Partner & COO, London
Ant is a Partner of Wallbrook and our Chief Operating Officer. He oversees our international operations, with a particular focus on our data privacy and protection.
An Arabist by training, Ant studied in the UK and Syria before starting as an Analyst with Billiter. He focused on Middle Eastern investigations, ranging from quick turnaround red flag screening through to sensitive cross-border disputes. He has particular experience in mapping the private interests of the Gulf's royals and business families, and exploring the risks that such relationships can pose.
Ant was part of the Billiter team that in 2013 was acquired by Stroz Friedberg and became its Due Diligence and Strategic Research unit. While continuing to support the MENA group he went on to manage DDSR's global open-source intelligence practice, managing relationships with multinational banks, corporates, and law firms, and running teams producing thousands of anti-money laundering, anti-corruption and reputational screens for them per year. Latterly he worked with senior compliance executives at such clients, helping them meet their regulatory responsibilities and advising on their programmes. This combination of research expertise, client insight and regulatory exposure saw Ant develop specialist expertise in privacy issues within due diligence, and he led the group's GDPR assessment ahead of its implementation in May 2018.
Ant brings this experience to his role as our COO – coordinating our regional operations and practice areas, driving our growth and implementing our robust data protection and privacy programme.
He holds a bachelor's in Arabic and Middle Eastern Studies from the University of Durham, and a master's in International Relations from the University of Manchester. He speaks Arabic.


Chicago's largest "Craft Beer Party in the Park" is BACK and will once again be hosted by Illinois Craft Brewers Guild within the beautiful Welles Park.
Designated drivers or non-beer drinkers CAN access the fest without a ticket. We reserve the right to remove anyone found sharing beer. Dogs are also welcome as long as they're leashed.


Since I started my job in Albuquerque, I've been thinking about a new house. And decorating a new house. So I've started stalking various DIY and home decorating blogs. It might have a little to do with Pinterest.
And since Mike just accepted a job in Albuquerque, house hunting is closer than ever! We've visited a realtor, have a couple of improvements scheduled (yard clean up, house painting, etc.) so we can sell our current house. We've never been house shopping before...we fell in love with this house, and everything worked out nicely...but yeah. Never actively looked for a house before. So now we have to figure all kinds of things out. We've got a couple of areas scoped out, since they're close to where we work.
I figure I want a biggish kitchen, area for entertaining, and some kind of patio. So I can have big brunches once a month for all my friends. And a clothesline. Which means no HOA. Enough garage so I can make furniture would be cool too.
FRICK! THIS IS SO EXCITING!!!


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Panamomus sawadai es una especie de coleóptero de la familia Endomychidae.

Distribución geográfica
Habita en Japón.

Referencias

Endomychidae
Insectos de Japón

redpajama_set_name: RedPajamaWikipedia


If you've visited my IG recently, you'll notice I like to post a bunch of photos with bright white backdrops. It's something I wanted to play with and the opportunity came about when I worked on a project with a recent client. So I shot a ton of content for my page and honestly I love it. I still love bright punches of color and it is still saturated instead of muted like some bright white images you may see out there. Someone asked me how I achieve bright white backdrops and so I figured I'd share (some) of my secrets. Keep reading for more!
For this example, I've decided to use a photo I've used to show the progression.
This picture is before editing. I take pictures with my iPhone 6s. The camera rivals that of a DSLR and for the purpose of my stock images & IG content, it works pretty well.
Natural light is great to use simply because it shows the true intensity and texture of what is being shot. If you want to manipulate natural light, invest in a soft light. I purchased mine on Amazon.com. In addition, I take aerial shots, I like to have the light directly above my objects for lighting consistency. Sometimes there are shadows and to help mitigate that, I use a reflector. I purchased my on Amazon.com also. A reflector serves as a fill light, meaning it fills light where there is a shadow. Looking at the image above, there are limited shadows and it really sets up my photo for making editing easier.
Once I've taken the picture, I begin the editing process! The fun part. Before I put it in any apps, I actually use the Edit feature in iPhone photos. It has the ability to add a filter and manipulate certain features of the photo: Light, Saturation & Black/White Balance. I usually use the Chrome filter to punch up the color all together and then go into the Light feature to manipulate Exposure, Contrast, Shadows, Brightness, Black Point, and Highlights. Below is what the image looks like after.
What I love about the iPhone edit feature is that you can do simple edits and still keep the photo's integrity intact. But I want to brighten up my white backdrop a little bit more. If you tried doing this in iPhone Edit, like increase the exposure, it would blow out the image and brighten everything, making it somewhat distorted and having everything blend in. So, I go to my biggest trick in the book, Snapseed.
Snapseed is a photo editing app with so many cool features, but we're going to talk about the Selective feature.
The Selective feature is great because you can edit specific parts of your image, and maintain it's integrity. Because I just want to focus on the bright white background, I use the selective feature to brighten certain parts. Below is an image after I've brightened certain areas of the image.
Once I've completed my updates. Now I'm ready to upload and post!!
It certainly takes practice to achieve the look you want. I didn't achieve bright white backdrops overnight and nor should you feel overwhelmed to do so. There's a learning curve for everyone!
Anywho, like what you've read? Sign up for our mailing list to get more exclusives as well as free stuff, like stock images!


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Chlamydiology and Rickettsiology
Specimen Processing and Concentration of Chlamydia trachomatis Added Can Influence False-Negative Rates in the LCx Assay but Not in the APTIMA Combo 2 Assay When Testing for Inhibitors
S. Chong, D. Jang, X. Song, J. Mahony, A. Petrich, P. Barriga, M. Chernesky
S. Chong
McMaster University and Father Sean O'Sullivan Research Centre, St. Joseph's Healthcare, Hamilton, Ontario
For correspondence: chernesk@mcmaster.ca
D. Jang
X. Song
J. Mahony
A. Petrich
P. Barriga
Sainte-Justine Hospital, Montreal, Quebec, Canada
M. Chernesky
DOI: 10.1128/JCM.41.2.778-782.2003
Inhibitors in clinical specimens can be detected by adding the target of nucleic acid amplification to the sample. Introduction of a Chlamydia trachomatis L2 434 preparation containing 12 elementary bodies (EBs) into first-void urine (FVU) from 225 nonpregnant women and 190 pregnant women before specimen processing by the assays produced false-negative rates of 0.48% (2 of 415 specimens) and 13% (44 of 338 specimens) by the APTIMA Combo 2 and the Chlamydia LCx tests, respectively. Reducing the amount of C. trachomatis added to one EB, a concentration closer to the APTIMA Combo 2 test cutoff, for a subset of 244 FVU specimens increased the number of specimens with false-negative results by the APTIMA Combo 2 assay to 7 (2.9%), suggesting that the strength of the input C. trachomatis per specimen has an influence on the number of specimens with false-negative results. Repeat testing after overnight storage and dilution decreased the APTIMA Combo 2 test false-negative rates to 0% (0 of 415 specimens) with the stronger inoculum and 0.8% (2 of 244 specimens) with the weaker inoculum; the false-negative rate of the LCx assay was reduced to 5.4% (18 of 334 specimens). When an additional 70 FVU specimens from women to which 12 EBs were added before specimen processing were tested by the LCx assay, 34 specimens had false-negative results, whereas 21 specimens had false-negative results when the C. trachomatis EBs were introduced after processing. Nine of the 21 specimens to which EBs were added after processing and all of the 34 urine specimens to which the target was added before processing remained falsely negative on repeat testing at a 1:2 dilution, suggesting that input C. trachomatis DNA was lost during processing by the LCx assay. In contrast, the APTIMA Combo 2 assay appears to have a higher sensitivity and either lost little nucleic acid during processing or demonstrated few problems with inhibitors of transcription-mediated amplification.
Chlamydia trachomatis is the most common sexually transmitted bacterium. Infections are often asymptomatic and if left untreated can cause pelvic inflammatory disease with sequelae of ectopic pregnancy, infertility, or chronic pelvic pain in women (3, 4).
Nucleic acid amplification (NAA) techniques are sensitive tools for the identification of C. trachomatis infections. While conventional tests require the invasive collection of specimens such as cervical or urethral swab specimens, NAA assays have been applied to first-void urine (FVU), vulvar, and vaginal specimens; however, some of these specimens contain substances which may contribute to false-negative results (10, 11, 14), and the results may also be influenced by pregnancy (9, 10). The false-negative results may be due to the presence of inhibitory substances in clinical specimens, the concentration of C. trachomatis added to the specimen, and/or the loss of nucleic acid from the inoculum during processing of the sample.
The APTIMA Combo 2 assay (Gen-Probe Incorporated, San Diego, Calif.) is a transcription-mediated amplification (TMA) method that uses a target capture technology during specimen processing to selectively isolate target C. trachomatis and Neisseria gonorrhoeae nucleic acids from potentially inhibitory biological substances in specimens. The test can be performed for the detection of one or both bacterial infections. In this study, we report on the prevalence of false-negative results detected when different concentrations of C. trachomatis elementary bodies (EBs) were added to FVU specimens collected from both pregnant and nonpregnant women and tested by the APTIMA Combo 2 assay and the Chlamydia LCx assay (Abbott Laboratories, Chicago, Ill.). We evaluated overnight storage at 4°C and/or dilution of the specimen as mechanisms for removing inhibitory activity. Experiments were also performed to compare the false-negative rates by the LCx assay when C. trachomatis EBs were added to FVU before processing and after processing.
Specimens.Specimens of FVU (defined as the first 10 to 30 ml of urine collected at any time) from 225 nonpregnant women submitted to a hospital chemistry laboratory for routine urinalysis and 190 pregnant women attending an obstetrics clinic were aliquoted and anonymously included in the study. No consent or patient information was collected. In the first series of experiments, all FVU specimens were tested with and without the addition of C. trachomatis EBs to the sample before processing by the APTIMA Combo 2 and the Chlamydia LCx assays. In the second series of experiments, 70 urine specimens from nonpregnant women were tested by adding two different concentrations of C. trachomatis EBs before and after specimen processing by the LCx test.
Determination of input C. trachomatis concentration.C. trachomatis L2 434 was propagated in McCoy cells for 72 h, and the EBs were purified by differential centrifugation as described previously (6). Serial 10-fold dilutions from 100 to 10−8 were made in phosphate-free saline, and the EBs were counted by direct fluorescent-antibody staining with monoclonal antibodies specific for major outer membrane proteins of C. trachomatis (Syva Behring Microtrak, San Jose, Calif.). The 10−4, 10−5, and 10−7 dilutions contained an average of 100, 12, and 1 EB(s) per 100 μl, respectively. Aliquots of the resuspended EBs in saline were prepared and frozen at −70°C.
Serial 10-fold dilutions of an EB preparation in saline were tested by both the APTIMA Combo 2 and the LCx assays in replicates of 16 to determine a concentration closest to the cutoff value of the respective tests. A volume of 100 μl of the last dilution (10−5) that yielded a positive signal by the LCx assay for all 16 replicates (approximately 12 EBs) was added to the 415 FVU specimens prior to testing by both the APTIMA Combo 2 and the LCx assays. The C. trachomatis preparation of 10−7 (approximately one EB), a concentration that is closer to the APTIMA Combo 2 test cutoff and that yielded a positive signal for all 16 replicates, was also added to a subset of 244 FVU specimens for testing only by the APTIMA Combo 2 assay. To measure the impact of adding different concentrations of C. trachomatis to the sample and the effects of the potential loss of nucleic acids during the specimen processing by the LCx assay, we tested a set of 70 FVU specimens to which 12 EBs (10−5 dilution) and 100 EBs (10−4 dilution) were added before and after the processing step of the assay.
False-negative results.A spiked specimen was considered falsely negative if the value recorded by the test was below the assay cutoff.
Removal of inhibitory substances.Spiked FVU specimens found to be inhibitory in the APTIMA Combo 2 and Chlamydia LCx assays were stored overnight at 4°C and retested the next day undiluted and at a dilution of 1:2 or 1:4.
Amplification assays.The Chlamydia LCx assay was performed as outlined by the manufacturer's protocol. One milliliter of FVU was centrifuged at ≥9,000 × g for 15 min. The resulting pellet was resuspended with 1 ml of the LCx assay urine resuspension buffer, and the suspension was heated at 97°C for 15 min. After the specimen was cooled at room temperature for 15 min, 100 μl was transferred to the appropriate LCx assay amplification vial. Cycling conditions were 40 cycles of 93°C for 1 s, 59°C for 1 s, and 62°C for 1 min and 10 s. Amplification products were detected with the LCx assay analyzer.
For the APTIMA Combo 2 assay, 400 μl of the FVU specimen was added to 100 μl of Target Capture Reagent-Solution B mix, and the mixture was incubated at 62°C for 30 min, followed by a second 30-min incubation at room temperature. During this step, the released C. trachomatis rRNA was bound to coated magnetic beads. The rack of tubes containing the rRNA-bound beads was subsequently placed on a magnetic target capture system for a series of washes, during which the captured target molecules were pulled to the side of the reaction vessel and the supernatant was removed. Seventy-five microliters of the APTIMA Combo 2 assay amplification reagent and 200 μl of oil were added to the specimen tubes, and the tubes were incubated at 62°C for 10 min. The tubes were cooled to 42°C, and 25 μl of enzyme reagent was added before the mixture was incubated for another hour at 42°C. The APTIMA Combo 2 assay probe reagent (100 μl) was added, followed by an incubation of 20 min at 62°C. At this point, single-stranded chemiluminescent DNA probes labeled with acridinium ester molecules were combined with the amplicon to form stable RNA-DNA hybrids. Finally, 250 μl of selection reagent, which differentiates hybridized from unhybridized probes, was added, and the tubes were incubated at 62°C for another 10 min. After the tubes were allowed to cool to room temperature, the light emitted from the labeled RNA-DNA hybrids was measured as photon signals in a Leader HC+ luminometer. The results were interpreted with computer software provided by the manufacturer.
Endpoint determination for each assay.The effects of serial 10-fold dilution of C. trachomatis L2 434 EBs in saline tested by the APTIMA Combo 2 and LCx assays are shown in Fig. 1. Endpoints of positivity were seen with 12 EBs (10−5 dilution) and 0.01 EBs (10−9 dilution) in the LCx and APTIMA Combo 2 assays, respectively. By the APTIMA Combo 2 test, there was a steep decline in readings, from a positive reading of 731,000 relative light units (RLU) at a dilution of 10−9 to a negative reading of 10,000 RLU at 10−10. A similar pattern was observed with the LCx assay signal/cutoff (s/co) ratio between endpoint dilutions of 10−5 to 10−7. While the LCx assay with 12 EBs (10−5) tested positive in 16 replicates, the last dilution to produce a positive signal in all 16 replicates of the APTIMA Combo 2 assay contained 1 EB (10−7) (Fig. 2).
Effect of serial 10-fold dilutions of C. trachomatis L2 434 (CT) in relation to the cutoff in the APTIMA Combo 2 and LCx assays. ▴, APTIMA Combo 2 assay; ▪, LCx assay.
Determination of endpoints showing all replicates of C. trachomatis L2 434 (CT) positive by the APTIMA Combo 2 and LCx assays. ▪, APTIMA Combo 2 assay; ▦, LCx assay.
False-negative results for urine specimens spiked before processing.A total of 415 FVU specimens collected from 190 pregnant women and 225 nonpregnant women were tested by the APTIMA Combo 2 and LCx assays with and without 12 C. trachomatis EBs. The data for one LCx assay run were eliminated from the data analysis due to invalid controls, thereby reducing the total number of FVU specimens tested by the LCx assay to 338 (159 pregnant women, 179 nonpregnant women). With an input C. trachomatis concentration of 12 EBs, which were introduced into the urine before processing, the APTIMA Combo 2 and LCx assays yielded negative results for 0.48% (2 of 415) and 13% (44 of 338) of the spiked specimens, respectively (Table 1). There was no significant difference in the number of spiked FVU specimens from pregnant and nonpregnant women with false-negative results by either assay (0 versus 0.89% for the APTIMA Combo 2 assay [P = 0.50], 11.9 versus 13.9% for the LCx assay [P = 0.58]).
Effect of adding C. trachomatis EBs to urine specimens from pregnant and nonpregnant women before processing by the APTIMA Combo 2 and LCx assays
When 244 of the FVU specimens were tested by the APTIMA Combo 2 assay after the addition of one EB before specimen processing, the proportions of samples with false-negative results increased to 2.5% (3 of 119 specimens) for pregnant women, 3.2% (4 of 125 specimens) for nonpregnant women, and 2.9% (7 of 244 specimens) overall. In these experiments, only 5 of 42 (11.9%) of the specimens with false-negative results by the APTIMA Combo 2 or LCx assay were falsely negative in both assays. None of the 42 specimens with false-negative results were from C. trachomatis-infected patients. Indigenous C. trachomatis nucleic acids were detected in 6 of 298 (2.0%) urine specimens with sufficient volume for testing by the APTIMA Combo 2 test and 2 of 223 (0.9%) urine specimens with sufficient volume for testing by the LCx assay.
Table 2 summarizes the results of repeat testing after overnight storage of the series of 415 urine specimens with false-negative results. Two urine specimens which yielded false-negative results by the APTIMA Combo 2 assay after the addition of 12 EBs and after storage at 4°C overnight gave a positive signal when they were tested the next day undiluted and diluted 1:4. Only two of seven urine specimens with false-negative results with a C. trachomatis inoculum containing one EB remained negative. There was sufficient volume to retest only 40 of the 44 urine specimens that were false negative by the LCx assay. Of these 40 specimens, 18 remained negative after overnight storage at 4°C and retesting at a 1:4 dilution. Thus, with storage and retesting after dilution, the false-negative rates were reduced to 0.8% for the APTIMA Combo 2 assay and 5.4% for the LCx assay.
Effect of storage and retesting of urine specimens to which C. trachomatis was introduced before processing by the APTIMA Combo 2 and LCx assaysa
Contribution of processing or inhibitory substances to false-negative results for spiked specimens.Table 3 summarizes the effect of adding 100 and 12 EBs of C. trachomatis before and after processing of 70 FVU specimens by the LCx assay. Whether C. trachomatis was introduced into the specimens before or after the processing step of the test protocol, fewer false-negative results were detected when 100 EBs were added, as only 2 of 70 urine specimens to which the EBs were added before and after processing tested falsely negative. When only 12 EBs were added, 30% (21 of 70) of the FVU specimens tested negative when C. trachomatis was added after processing, whereas 48.6% (34 of 70) of the FVU specimens tested negative when the target nucleic acid was added before processing. Repeat testing after overnight storage at 4°C and dilution of the sample 1:2 had the largest impact on the results for the specimens when the EBs were introduced after processing. Of the two samples that generated negative results after the addition of 100 EBs before processing, one retested positive the next day. None of the 34 samples for which false-negative results were detected when 12 EBs were added before specimen processing became positive on repeat testing. The two samples that were negative upon the addition of 100 EBs after processing retested positive, and 9 of the 21 FVU specimens that were false negative when 12 EBs were introduced after processing remained negative upon repeat testing the next day.
Effect of introducing two different concentrations of C. trachomatis EBs to urine before and after processing on the number of samples whose results fall below the test cutoff (false negative) by the LCx assay
The APTIMA Combo 2 assay was 100-fold more sensitive than the LCx test for the detection of serial dilutions of C. trachomatis L2 434 in saline. The last dilution to consistently produce a positive signal in 16 replicates of the APTIMA Combo 2 assay was 10−7 (1 EB), whereas for the LCx assay the last dilution was 10−5 (12 EBs). Clinical evaluations are needed to determine whether this greater analytical sensitivity of the APTIMA Combo 2 test can be translated to the analysis of patient specimens. In a study by Martin et al. (D. H. Martin, C. Cammarata, L. Ivor, K. Smith, and C. Knott, Abstr. 100th Gen. Meet. Am. Soc. Microbiol., abstr. C-387, 2000), in which an infected patient was defined as positive if the patient specimen was positive by any two NAA tests, the sensitivities for detection of C. trachomatis in swab and urine specimens were reported to be 86.7 and 86.7%, respectively, for the APTIMA Combo 2 assay and 85.0 and 60.0%, respectively, for the LCx assay. In another study (M. L. Theodore, N. Dalesio, B. J. Wood, T. C. Quinn, and C. Gaydos, Abstr. 39th Annu. Meet. Infect. Dis. Soc. Am., abstr. 926, 2001), 327 urine samples from both symptomatic and asymptomatic men and women were tested by the Abbott LCx assay, the Becton Dickinson ProbeTec ET assay, and the Gen-Probe APTIMA Combo 2 assay. With a "gold standard" for a positive result defined as positive results by any two NAA tests, the sensitivities for C. trachomatis detection were found to be 94.7% for the LCx assay and 100% for the APTIMA Combo 2 assay.
Previous studies defining inhibition rates for NAA assays have tested specimens to which C. trachomatis inoculum near the cutoff value for each test was added; results below the test cutoff for positivity were presumed to be due to the presence of inhibitors in the specimen (10, 14). Those studies introduced C. trachomatis after processing. In the APTIMA Combo 2 assay, a raw EB preparation cannot be added after processing because it is only at the processing step when the cells are lysed and the bacterial rRNA is extracted.
Addition of 12 C. trachomatis EBs to each specimen before processing showed a false-negative rate of 0.48% (2 of 415 specimens) by the APTIMA Combo 2 assay. Seven of 244 (2.9%) FVU specimens to which one EB of C. trachomatis was added tested negative. This observation indicates the importance of testing specimens with C. trachomatis at a concentration near the cutoff value of an assay to provide a true indication of the test's susceptibility to amplification inhibitors. Inhibition may not be detected with an EB preparation that is too strong. On the other hand, if the inoculum is too weak, it may be lost during specimen processing, thereby showing an inflated inhibitor prevalence. The latter scenario may have occurred in the LCx assay. When the EBs were added before processing, 13% (44 of 338) of the spiked samples tested negative. In a previous study, we added C. trachomatis to the specimens after processing and found a lower inhibition rate of 3.9% (10), with all of the initial negative samples retesting positive after overnight storage and dilution. Other investigators have found similar inhibition rates. Berg et al. (2) detected an inhibition rate of 2.6% with urine specimens collected from men attending a sexually transmitted disease clinic. Gaydos et al. (8) reported no LCR inhibitors in urine specimens from 148 pregnant women. The urine specimens from the last two studies were either frozen at −70°C or stored at 4°C for days prior to testing with a C. trachomatis inoculum. While this may account for the lower rates because some inhibitors of the LCx assay or PCR are labile with storage (10, 14), in those studies C. trachomatis was also introduced into the specimens after specimen processing.
False-negative results may be observed in NAA tests when the number of targets in a sample is low because of sampling variability. When inhibitors are responsible for false-negative NAA test results, they may interfere with cell lysis, nucleic acid extraction, or target capture, causing nucleic acid degradation. Alternatively, they may interfere with the enzyme activity required for amplification (15). The larger number of false-negative results observed in the LCx assay in the present study may have been created by the step in the testing protocol at which the C. trachomatis EB inoculum was added to the specimen. Experiments with 70 urine specimens to which C. trachomatis was added before processing and after processing (Table 3) showed that fewer false-negative results were found when the EB preparation was added after specimen processing than before specimen processing (21 versus 34). Examination of the LCx assay s/co ratios (data not shown) for the 34 samples to which EBs were added before the specimen processing step of the test protocol revealed that the s/co ratio for none of them was near the LCx assay cutoff of 1.0 (s/co ratio range, 0.02 to 0.32). On the other hand, of the 21 specimens with false-negative results that were identified when EBs were introduced to the samples after processing, 12 retested positive at a 1:2 dilution. Eight of the nine samples that retested negative were on the borderline of positivity (s/co ratio range, 0.67 to 0.98; mean, 0.85). Although the number of specimens containing 100 C. trachomatis EBs with false-negative results was small, the two samples that were identified when the inoculum was added after processing retested positive, whereas only one of two false-negative specimens became positive when EBs were added before processing became positive upon repeat testing. These important observations indicate that a loss of positivity may be due to the presence of inhibitors in the sample and/or the loss of nucleic acids during specimen processing when C. trachomatis is added to the urine before initiation of the test protocol.
There were no differences in the false-negative rate of either the LCx assay or the APTIMA Combo 2 test for urine specimens from pregnant and nonpregnant women. In a previous study (10), the inhibitor prevalence rates for the LCx assay were 1 and 3.1%, respectively, for urine specimens from 101 pregnant women and 287 nonpregnant women. Pregnancy also caused no difference in the inhibitor prevalence rate for the Amplified CT TMA test assessed in that study. Only PCR was inhibited by urine from pregnant women more often than it was inhibited by urine from nonpregnant women (9.9 versus 3.1% [P = 0.011]) (10). Jensen et al. (9) reported that the inhibitor prevalence rate for urine samples in the LCx assay was greatly affected by the pregnancy status of 1,136 women because 15 cervical swabs were positive for C. trachomatis by direct fluorescent-antibody staining-confirmed enzyme immunoassay, but the corresponding urine samples were negative by the LCx assay. As the specimens were not tested with a C. trachomatis inoculum, the differences may be accounted for by inconsistent sampling or loss of nucleic acids during processing of the urine specimens. To definitively test for amplification inhibitors, C. trachomatis needs to be added to the clinical specimen after specimen processing.
Of the C. trachomatis-spiked urine specimens, only 5 of 42 tested false negative by both the APTIMA Combo 2 and LCx assays, suggesting that if all specimens truly contained inhibitors, the substances and/or the mechanisms causing amplification inhibition are different. Urinalysis and microscopic examination of both inhibitory and noninhibitory urine specimens in a previous study (10) showed a strong association between the presence of certain urinary substances and inhibition of various amplification assays. Logistic regression analysis of the data revealed that beta-human chorionic gonadotropin and crystals are associated with PCR inhibition, that nitrites are associated with LCR inhibition, and that hemoglobin, nitrites, and crystals are associated with TMA method inhibition. PCR has also been shown to be inhibited by bile salts and complex polysaccharides in feces, heme in blood, and urea in urine (1, 15), while the LCx assay has been inhibited by phosphate (11).
When 12 EBs were added to the samples, after overnight storage at 4°C, none of the urine specimens with false-negative results by the APTIMA Combo 2 assay remained negative. Only two of the seven (28.6%) urine specimens found to be negative with the addition of one EB remained negative after retesting. The false-negative rate for the LCx assay was lowered from 13 to 5.7% after storage. This finding is similar to those reported in other studies, in which amplification inhibitors were removed by dilution, prolonged storage of the specimen prior to amplification, or freezing and thawing (2, 5, 7, 8, 10, 12, 13).
Several important principles have been demonstrated in these studies. The sensitivities of NAA tests for the detection of nucleic acids may fall short of 100% because of the presence of inhibitors in clinical specimens and/or the loss of low levels of nucleic acids during specimen processing. Inhibitor prevalence rates measured after the addition of C. trachomatis to the specimens after sample processing are a truer estimate because the loss of nucleic acids during processing is not a factor. The addition to a specimen of a C. trachomatis concentration that is too close to the test's cutoff runs the risk of loss of nucleic acids and the creation of artificially negative results which may be assumed to be the result of the presence of inhibitors, whereas use of an EB preparation with a concentration that is too strong may overpower any inhibitors in the sample.
We have found the prevalence of NAA test inhibitors in urine specimens tested by the Gen-Probe APTIMA Combo 2 assay to be very low (range, 0.48 to 2.9%). The rate was dependent upon the amount of C. trachomatis EBs added to the FVU specimens prior to the processing step of the assay. None of the inhibitory urine specimens detected were from infected patients, and there were no differences in rates of inhibitory activity on the basis of pregnancy. Most of the inhibitory activity detected in the APTIMA Combo 2 assay was removed by overnight storage at 4°C. With this low susceptibility to amplification inhibitors and a high sensitivity level, the APTIMA Combo 2 assay has the potential to detect additional cases of C. trachomatis in urine specimens.
We thank the nurses of the St. Joseph's Healthcare Centre Obstetrics and Gynecology Department, Michael St. Pierre, and the technologists of the Chemistry Department of St. Joseph's Healthcare Centre for collecting urine specimens for this study.
Received 9 April 2002.
Returned for modification 31 July 2002.
Accepted 12 November 2002.
Abu Al-Soud, W., and P. Radstrom. 1998. Capacity of nine thermostable DNA polymerases to mediate DNA amplification in the presence of PCR-inhibiting samples. Appl. Environ. Microbiol. 64:3748-3753.
Berg, E. S., G. Anestad, H. Moi, G. Storvold, and K. Staug. 1997. False-negative results of a ligase chain reaction assay to detect Chlamydia trachomatis due to inhibitors in urine. Eur. J. Clin. Microbiol. Infect. Dis. 16:727-731.
Cates, W., and J. N. Wasserheit. 1991. Genital chlamydia infections: epidemiology and reproductive sequelae. Am J. Obstet. Gynecol. 164:1771-1781.
Centers for Disease Control and Prevention. 1993. Recommendation for prevention and management of Chlamydia trachomatis infections. Morb. Mortal. Wkly. Rep. 42:1-39.
Chernesky, M., S. Chong, D. Jang, K. Luinstra, M. Faught, and J. Mahony. 1998. Inhibition of amplification of Chlamydia trachomatis plasmid DNA by the ligase chain reaction associated with female urines. Clin. Microbiol. Infect. 4:397-400.
Chernesky, M. A., D. Jang, J. Sellors, K. Luinstra, S. Chong, S. Castriciano, and J. B. Mahony. 1997. Urinary inhibitors of polymerase chain reaction and testing of multiple specimens may contribute to lower assay sensitivities for diagnosing Chlamydia trachomatis infected women. Mol. Cell. Probes 11:243-249.
de Barbeyrac, B., M. Geniaux, C. Hocke, M. Dupon, and C. Bebear. 2000. Detection of Chlamydia trachomatis in symptomatic and asymptomatic populations with urogenital specimens by AMP CT (Gen-Probe Incorporated) compared to others commercially available amplification assays. Diagn. Microbiol. Infect. Dis. 37:181-185.
Gaydos, C. A., M. R. Howell, T. C. Quinn, J. C. Gaydos, and K. T. McKee, Jr. 1998. Use of ligase chain reaction with urine versus cervical culture for detection of Chlamydia trachomatis in an asymptomatic military population of pregnant and nonpregnant females attending Papanicolaou smear clinics. J. Clin. Microbiol. 36:1300-1304.
Jensen, I. P., P. Thorsen, and B. R. Moller. 1997. Sensitivity of ligase chain reaction assay of urine from pregnant women for Chlamydia trachomatis. Lancet 349:329-330.
Mahony, J. B., S. Chong, D. Jang, K. Luinstra, M. Faught, D. Dalby, J. Sellors, and M. A. Chernesky. 1998. Urine specimens from pregnant and nonpregnant women inhibitory to amplification of Chlamydia trachomatis nucleic acid by PCR, ligase chain reaction, and transcription-mediated amplification: identification of urinary substances associated with inhibition and removal of inhibitory activity. J. Clin. Microbiol. 36:3122-3126.
Notomi, T., Y. Ikeda, A. Okadome, and A. Nagayama. 1998. The inhibitory effect of phosphate on the ligase chain reaction used for detecting Chlamydia trachomatis. J. Clin. Pathol. 51:306-308.
Pasternack, R., P. Vuorinen, and A. Miettinen. 1997. Evaluation of the Gen-Probe Chlamydia trachomatis transcription-mediated amplification assay with urine specimens from women. J. Clin. Microbiol. 35:676-678.
Stary, A., S. Tomazic-Allen, B. Choueiri, J. Burczak, K. Steyrer, and H. Lee. 1996. Comparison of DNA amplification methods for the detection of Chlamydia trachomatis in first-void urine from asymptomatic military recruits. Sex Transm. Dis. 23:97-102.
Verkooyen, R. P., A. Luijendijk, W. M. Huisman, W. H. F. Goessens, J. A. J. W. Kluytmans, J. H. van Rijsoort-Vos, and H. A. Verbrugh. 1996. Detection of PCR inhibitors in cervical specimens by using the AMPLICOR Chlamydia trachomatis assay. J. Clin. Microbiol. 34:3072-3074.
Wilson, I. G. 1997. Inhibition and facilitation of nucleic acid amplification. Appl. Environ. Microbiol. 63:3741-3751.
Journal of Clinical Microbiology Feb 2003, 41 (2) 778-782; DOI: 10.1128/JCM.41.2.778-782.2003
You are going to email the following Specimen Processing and Concentration of Chlamydia trachomatis Added Can Influence False-Negative Rates in the LCx Assay but Not in the APTIMA Combo 2 Assay When Testing for Inhibitors


This new section is our photo album of announcers and their stations from years gone by and currently in action throughout the communities If you have any photos that you would like to add to this section, from today or years gone by...just e-mail it to "ON THE AIR", or mail it to P.O Box 196, Eastern Passage, Nova Scotia, B3G 1M5, and it will be scanned to the site!
Here's some interesting bits of promo items from years gone by, that were donated for the "On the Air" photo album.......Please be patient as these photo will take some time to load in.
Here's a bit of history! 790/CFDR reel to reel box cover with the voice tracks of the all night program, Music In The Night" with broadcaster, John Cunningham. CFDR was was located at 790 on the AM band, with a power out put of 5,000 watts. The station was located at 12 Queen Street in Dartmouth.
Back in September 1987, CFDR which at that time broadcast at 680kHz was taking over by a new company, Newcap from Pattersons Broadcasting. What came with the new company was also a new format from easy listening to top 40. The following is an editorial cartoon which appeared in what then was the Dartmouth This Week newspaper.
Nothing like visiting good radio pals and having some pizza! That's what broadcaster Paul Marr did in this picture from 1994. Here's Paul sitting in the master control room of CHNS-AM 960, when it was located at 1313 Barrington Street, Halifax.
From 1986, comes this photo of the on air gang at Halifax's Country 101-CHFX FM. This photo was taken in 1986 and appeared on the back cover of a station's promotional music album from MCA Records, titled "Our Country Is Super". Notice the on air shifts of the broadcasters.
Still with CHNS in Halifax, here's a book mark with pictures of some of the jocks that were on the air in 1969. On the flip side is the CHNS official survey for the week of Jan. 31st - Feb. 7th, 1969.
What a way to let your listeners know about your station! The following is the cover of the CFDR's Airwaves Newsletter. At the time CFDR was at 680 on the AM dial and located in Dartmouth . There's no date on this promo item, but I've been told it's could be around 1980. That's the time that CFDR moved into their new home on top of Queen Square, which after three years their sister station CFRQ-Q104 arrived on the air!
The following picture was part of PETER DUFFY's column that appeared in the February 17th/2000 editon of the Halifax Chroncile-Herald, under the title "Radioland Debut Bit Of A Nerve-Racking Experience"
JC DOUGLAS BEHIND THE Q104 "ROCK OF THE ATLANTIC" MIC AT THEIR QUEEN STREET LOCATION IN DARTMOUTH. JC ONCE DID THE 6 PM TILL MIDNIGHT SHIFTS ON SATURDAY'S AND SUNDAY'S EVENINGS FROM 1986 TILL EARLY 1988.
The AVR Radio Network van parked outside the Halifax World trade And Convention Centre.


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Cutting Edge: Modernist British Printmaking, Dulwich Picture Gallery review - a cut above
Under a turbulent sky racked with jagged clouds suggesting bolts of lightning, pale figures hurl themselves into a spitting expanse of water. Swathed in white towels, other figures mingle with the pink bodies, seeming to process along the pier as if...
Tove Jansson (1914-2001), Dulwich Picture Gallery review – more than Moominvalley
Born into an artistic Swedish-speaking household in Helsinki, Tove Jansson's first, and most enduring, ambition was to be a painter. Although best known as the illustrator behind the creatures of Moominvalley, those plump white hippopotamus-like...
Sargent, Dulwich Picture Gallery review - wonders in watercolour
This sparkling display of some four score watercolours from the first decade of the last century throw an unfamiliar light on the artistry of John Singer Sargent (1856-1925), the last great swagger portrait painter in the western tradition. None...
Vanessa Bell, Dulwich Picture Gallery
The Other Room, dating from the late 1930s, is the largest painting in Dulwich Picture Gallery's landmark retrospective, the first show to be dedicated to Vanessa Bell since a posthumous Arts Council show in 1964. In it, three women inhabit a space...
Best of 2016: Art
Before we consign this miserable year to history, there are a few good bits to be salvaged; in fact, for the visual arts 2016 has been marked by renewal and regeneration, with a clutch of newish museum directors getting into their stride, and...
Adriaen van de Velde, Dulwich Picture Gallery
Oh, those dogs: just a flick of the brush, and there they are, bursting with life. Pets, hunting dogs, companions, strays: romping on beaches, or in Dutch forests, living on farms and in imagined arcadias. Adriaen van de Velde was a 17th century...
Andsnes and Friends 2, Dulwich Picture Gallery
Nature, nationalism, folk culture: the broad themes of Norway's visual arts map easily onto its music. That has given Leif Ove Andsnes and his colleagues plenty of leeway in planning their musical tributes to the painter Nikolai Astrup. For this,...
Nikolai Astrup: Painting Norway, Dulwich Picture Gallery
Dulwich Picture Gallery, the oldest public painting gallery anywhere with one of the world's finest collections of Old Masters, has in recent years built up a deserved reputation for bringing to the British audience unfamiliar aspects of well known...
The Amazing World of MC Escher, Dulwich Picture Gallery
Walls that are floors, floors that are walls, and stairs that go up to go down: in the brain-befuddling art of MC Escher (1898-1972) the mundane everyday meets a world of paradox in which the rules of gravity, space and material reality are thrown...
Ravilious, Dulwich Picture Gallery
Look at me, and think of England. This marvellous array of quirky, idiosyncratic watercolours by Eric Ravilious (1903-1942) from the 1930s until his premature death during wartime when his plane, on an air sea rescue mission for which he had...
Emily Carr, Dulwich Picture Gallery
Walking into this exhibition is a bit like walking into a great forest. The dark green walls are hung all around with paintings of trees; we look up through branches that spiral dizzyingly skyward, while the upwards sweep of vast trunks seem...
Art and Life: Ben and Winifred Nicholson, Dulwich Picture Gallery
At the risk of sounding crass, I can't help feeling that had Winifred Nicholson painted fewer flowers she might be better represented in the annals of art history. Of course, being a woman hasn't helped, but as a woman flower painter she was ever...


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What Body Of Water Is Pink?
A brilliant pink body of water may be seen on the point of the Yucatan Peninsula in Mexico, which appears to be from another planet. Las Coloradas, which translates as 'blush red' in Spanish, gets its distinctive coloring from red-colored algae, plankton, and brine shrimp that live in saline water and contribute to the magenta pink color of the water.
A brilliant pink body of water may be found on the point of Mexico's Yucatan Peninsula. It is unlike anything else on the planet. Las Coloradas, which translates as 'blush red' in Spanish, gets its distinctive coloring from red-colored algae, plankton, and brine shrimp that live in the saline water and contribute to the magenta pink color of the water.
Why does my water look pink?
What is causing my water to seem pink? Avoid being too frightened if you pour yourself a glass of water one day and notice a stream of brilliant pink water flowing out of it. Water running pink from the faucet is not unheard of, and it is frequently caused by the chemical potassium permanganate, which is used by municipal water treatment plants to clean their water systems.
Where are the Pink Lakes in the world?
My water is becoming pink.Why is this happening?Avoid being too frightened if you pour yourself a glass of water one day and notice a stream of brilliant pink water flowing.Water running pink from the faucet is not unheard of, and it is frequently caused by the chemical potassium permanganate, which is used by municipal water treatment plants to disinfect their water systems and supply lines.
What is Himalayan pink salt water?
It is worth noting that such water is composed of a saturated brine solution of unrefined, natural salt known as Himalayan pink salt, which is obtained from the Himalayan mountains (HPS). In this solution, electrolytes are supplied to the body, which assists in the regulation of the hydration process.
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What ocean is pink?
It is the hue of bright-pink bubble gum at Lake Hillier on Middle Island, Australia. Researchers have just revealed that algae, halobacteria, and other microorganisms are responsible for the lake's distinctive coloration. Also noteworthy is the fact that this body of water is very salty—almost as salty as the Dead Sea.
Is there a lake that is pink?
Consider the color pink. Lake Hillier, located on Middle Island, off the southern coast of Western Australia, is one of the country's most renowned pink lakes and one of the world's most photographed landscapes. It was in 2015 that a team of researchers from the Extreme Microbiome Project (XMP) made the decision to launch a study into the source of the lake's vibrant pink tint.
Is there a pink lake in the US?
If you can't get enough of the bright colors and want to see something truly unique, we've got something special in store for you. Undoubtedly one of the best hidden beauties in all of Utah is a pink lake with a pink tinge to it. According to Live Science, the Great Salt Lake in Utah is one of the biggest lakes west of the Mississippi River and is the largest lake in the United States.
Is there a pink water beach?
The beach that is dyed pink is genuine, and it is located in the hamlet of Ro Lagartos, despite how bizarre it appears. The name of this magnificent spot, which appears to have been plucked straight from a fairy tale, was given to it because of the pink tint of the water. The phrase ″Las Coloradas″ may be translated as ″the coloured ones,″ and it refers to the water.
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Why is pink lake not pink?
The pink halobacterium is found in the salt crust near the lake's bottom, where it thrives. Because of the building of the South Coast Highway and a rail line, it is thought that the flow of water into the lake has been changed, resulting in a reduction in salinity, which is why the lake no longer appears pink (as of 2017).
Why is pink lake pink?
When a lake is red or pink in color, it is said to be a pink lake. In most cases, the existence of salt-tolerant algae that generate carotenoids, such as Dunaliella salina, in association with certain bacteria, which might vary from lake to lake, is responsible for this.
Can you swim in pink lake?
Is it possible to swim in Pink Lake Australia? Swimming in the water of the lake is safe and enjoyable, but it is not feasible for ordinary visitors to do so because the lake cannot be reached.
Why are salt mine water red?
Thousands of minute, unicellular creatures dwelling in the water and salt crust are responsible for the reddish hue of the water and salt crust. We can't begin to imagine how they manage to endure the scorching summer heat and concentrated brine.
Does pink salt come from Pink Lakes?
For various reasons, including a high salt content as well as other factors, microorganisms dominate the environment in Pink Lake. These include red algae such as Dunaliella salina, which cause a high salt content in the lake as well as the production of a red dye that gives the lake its color, as well as red Halobacteria, which can be found in salt crusts.
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Why is salt water pink?
The lake and its salt crust have a characteristic pink tint because to the pigments contained in the cells of these salt-lovers, which include carotenoids such as those found in carrots.
Is pink sand real?
The pink color on Harbour Island in the Bahamas—one of the most famous beaches in the world—is derived from foraminifera, a tiny creature with a reddish-pink shell, and the sand is a mixture of coral, shells, and calcium carbonate, according to the National Geographic website.
What makes pink sand?
A coral reef nearby provides the pink sand beaches with the hue they have because of the hundreds of broken coral fragments, shells, and calcium carbonate components left behind by foraminifera (small marine animals with red and pink shells) that reside in surrounding coral reefs.
How does the sea turn pink?
This beautiful video demonstrates how a sea may develop a brilliant pink color as a result of the high salt content of the water. The brilliant pink hue is created by colorful salt-loving microorganisms that produce colored protein in order to absorb the sun's energy. The color is only visible for two months out of the year since the microbes are only active during the day.
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Gales, Weston R, address before So- 138 n, 142.
Gaston, William, at examinations of ketch, 617, 620.
Gay, John L., 399. editor of Baptist Messenger, 269 n.
Gilliam, Francis, student, surgeon on "Here's to Wake Forest," 432.
Gore, D. N., beneficiary, sketch, 614; Hester, J. G., receipts for books, 521.
Gordon, W. R., student, lawyer, 640. 454.
Gorrell, A. B., adjutant in C. S. A., Hill, W. L., 331.
665. Hinton, G. J., alumnus, physician, 636.
Gorrell, J. H., "History of Grounds" Hobgood, Wm., beneficiary, 612.
quoted, 105. Hofwyl, manual labor school, 7.
Graham, Stephen, on committee to Holding, Isham, buys land, 195 ff.
Graves, Calvin, 374. ship, 276, 329.
Graves, George A., alumnus and Honorary members of Societies, 499 ff.
planter, 435 n, 651; captain in Honeycutt, J. H., beneficiary, 611.
C. S. A., 665. Hooper, J., DeBerniere, 411.
manual labor, 89. 328, 410.
Green River Association, 325. speaker, 583, 602.
Greensboro Academy, charter, 55. Hooper, Mrs. William, 408, 471.
Greensboro Female College, charter, Horner, T. J., 318.
Gwaltney, W. R., 323, 461 n, 497, bama Baptist, 652.
509 n, 557; beneficiary, 615; chap- Hotel at Wake Forest, 113, 193, 199 f.
55 n, 59 f, and note.
Houston, W. J., captain, C. S. A., 665.
sketch, 613. 625; chaplain, C. S. A., 625.
Hampton Institute, 328 n. Howell, Mrs. J. K., 328.
Harris, Henry H., alumnus, physician, Howerton, W. J., 497.
Harrison, Carter, 603. paying debt, 251.


This 3-day Scrum course is designed to give a full view of Scrum and Agile Product Development. By the end of the course, delegates will be ready to take both the exam for both Professional Scrum Product Owner Certifications and Professional Scrum Master Certifications.
Delegates will understand the Scrum framework and how it can be applied to projects, understand the role of the Product Owner and that of a Scrum Master.
I am leading a programme of agile change within a large travel organisation - I have now applied many of the principles and ideas I developed on the course, to the teams I am working with to great effect.


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Peach Passion Sangria Cocktail Mix, 5.5 oz.
Mix up a pitcher of this deliciously sweet sangria and—Olé!—you're ready to host a fiesta. Simply add this dry mix made with the very best ingredients to white wine to make one gallon of the refreshing peach-flavored cocktail. This quick and easy beverage mix comes packaged in a vibrant paper tote tied with a mini wire whisk that makes it a perfect hostess gift or party favor.
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A Test Practice Lead / Test Coach will be the informed opinion leader and the go-to person for Quality Engineering and Testing. They will be a trusted source capable of inspiring others with modern thinking and be able to turn those innovative ideas into reality. The Test Coach will work closely with Product Owners, Stakeholders, Subject Matter Experts, Software Engineers, 3rd Party Suppliers, End Users and other leaders from across the client.
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A Procurement Manager with contractual, supply chain management and client facing experience in the Defence sector is required to work a permanent based in Hertfordshire. The main focus of the role is to develop strategic consolidation of the supply chain, meet ongoing cost reduction challenges and secure crucial orders and contracts with new and existing customers.


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Media Advisory 19-003
Media call: NSF Fiscal Year 2020 budget request
NSF staff to discuss proposed level of funding
Call will include information on the new budget proposal.
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Media are invited to join a 30-minute call about the President's Fiscal Year (FY) 2020 National Science Foundation (NSF) budget request to Congress.
The call will include information on the new budget proposal, the details of which are expected to be released on March 18, 2019. NSF staff will answer questions and speak to how agency investments in fundamental science and engineering research will continue to support the nation's economy, security and global leadership.
When: 4:30 p.m. E.T., March 18, 2019
Where: RSVP for call-in information with the subject line "Media call FY2020 request"
What: NSF staff will be available to answer questions about the budget request
RSVP: Sarah Bates, sabates@nsf.gov
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Sarah Bates, NSF, (703) 292-7738, email: sabates@nsf.gov
The National Science Foundation (NSF) is an independent federal agency that supports fundamental research and education across all fields of science and engineering. In fiscal year (FY) 2020, its budget is $8.3 billion. NSF funds reach all 50 states through grants to nearly 2,000 colleges, universities and other institutions. Each year, NSF receives more than 50,000 competitive proposals for funding and makes about 12,000 new funding awards.
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