 sar alegjaamdar fleming dwara pensilin ‌ nu kanugonadaniki dhaaritheesina prayoogam gurinchina yea paeraani batti ,  pensilin anty bacterial prabhavanni kaligi unna e rakamaina bacteriapy anaku cheppandi mariyu pensilin yokka aavishkaranhaku avasaramaina paristhithulanu jaabithaa chaeyamdi .
1927  natiki ,  fleming stephilokaki yokka lakshanaalanu parisoedhimchaadu .  athanu tana munupati pania nundi appatike bagaa prasiddi chendhaadu mariyu adbuthamaina parisoedhakudigaa khyaatini pemchukunnaadu . 1928 loo ,  athanu sahaja sthithilo perigina stephylocacus aureus yokka vaividhyaanni adhyayanam Akola ,  josep warwick bigger yokka pania tarwata ,  bacterium vividha rakaluga  ( jaathulu )  peruguthundani kanugonnaadu . 3  septembaru  1928 na ,  fleming tana kutumbamtho safolk ‌ loo selavu gadipi tana prayogasaalaku tirigi vachadu .  tana selavudinaaniki bayaludere mundhu ,  athanu kalture platelets ‌ lapai stephilokaakini teakaalu vaysi ,  vatini tana prayogashaalalo ooka moolalo unna bench ‌ pai vadilesaadu .  athanu tirigi vacchinappudu ,  fleming ooka samskruthi fungus ‌ thoo kalushitamaindani mariyu ventane fungus chuttuu unna stephilokaki yokka kaalaneelu naasanamayyaayani gamaninchaadu ,  ayithe dooramgaa unna itara stephilokaki kaalaneelu saadhaaranamainavi , " idi funny "  ani pramukhangaa perkonnaadu .  fleming tana maajii sahaayakudu merlin prise ‌ ki kalushitamaina samskruthini choopinchaadu ,  athanu  " ola meeru lysozyme ‌ ni kanugonnaru "  ani atanaki gurtu chesudu .  athanu achu pensilium jaatiki chendinadigaa gurtinchadu .  athanu dhaanini  P.  crisogenam ani anumaaninchaadu ,  ayithe ooka sahodyogi charless  J.  laaw touuch dhaanini  P.  rubram ‌ gaaa gurtincharu . ( tarwata idi  P.  notatam ‌ gaaa sarididdabadindi mariyu adhikarikamgaa  P.  crisogenam ‌ gaaa aamoedimchabadimdi ; 2011 loo ,  idi  P.  roobens ‌ gaaa parishkarinchabadindi .)
 fleming pensilin ‌ ni kanugonna mariyu parikshinchina prayogasaala paddington ‌ loni sint marys hospitaal ‌ loni alegjaamdar fleming laboratory museumgaa bhadraparachabadindi .  phangal kalushitham yokka muulam  1966 loo laaw touuch yokka gadi nundi vacchindi ,  idi fleming yokka neerugaa diguvana Pali .
 fleming swachchamaina samskrutilo acchunu pemchaadu mariyu samskruthi rasamlo anty bacterial padaardham undani kanugonnaadu .  athanu anek jeevulapai dani anty bacterial prabhavanni parisoedhimchaadu mariyu idi scarlett fever ,  nyumooniyaa ,  meningitis mariyu diftheriaku karanamayye stephilokaki mariyu anek itara graam - positive vyaadhikarakaalanu prabhaavitam chestundani gamaninchaadu ,  conei tiphoid jvaram ledha paratifaid jvaram kadhu .  graam - negative bactria ,  dani choose athanu aa samayamlo nivaarananu korutunnadu .  yea bacterium graam - negative ayinappatikee ,  idi goneriaku karanamayye neesseria gonorianu kudaa prabhaavitam chesindi . " achu rasam "  ledha  " nirodhakam "  ani pilichey konni nelala tarwata ,  athanu  7  marchi  1929 na acchulo unna anty bacterial padaarthaaniki pensilin aney peruu pettadu .
 pensilin meningitis ,  diptheria ,  nyumooniyaa ,  scarlett fever ‌ ku karanamayye byaakteeriyaato sahaa anek graam - positive bacteria yokka anty bacterial prabhavanni kaligi umtumdi .  idi  ( graam - positive )  stephilokaki bacteriapy kudaa pania chesthundu ,  idi charmam mariyu mrhudhu kanajaala in ‌ fection ‌ laku kaaranamavuthundhi .  ganeriyaaku karanamayye graam - negative bacteriapy pensilin anty bacterial charyanu kudaa kaligi unnatlu kanugonabadindi .  pensilin ‌ nu kanipettadaaniki veelu kalpinche adrushtavasaattuu anek paristhitulu unnayi ,  veetilo okati sar alegjaamdar tana kutumbamtho kalisi selavudinam choose enchukunnadi ,  nishkramanaku mundhu tana prayogashaalalo stephilokaki bacterianu samskrutilo vadilivesindi .  vruttaamtamgaa sar alegjaamdar laab parisubhratatoe komchem alasatvam vahinchaadu ,  idi atani munupati lysozyme aavishkaranhaku dhohadhapadindhi mariyu pensilin yokka aavishkaranhaku pakshikanga adae shradda lekapovadam kaaranamani thelusthondi .  chivaraga atani laab yokka nirdishta pradeesam mariyu samskruthulu ooka paathranu pooshinchaayi  -  stephilokaki samskrutipai anty bacterial prabhavanni kaligi unna phangal kalushitham sar alegjaamdar yokka sahacharula laab ooka anthastu nundi vacchindi .