This set of Biochemistry online test focuses on “ATP Synthesis – 2”.
1. The proposal of chemiosmotic hypothesis was by
a) Peter D. Mitchell
b) Charles Darwin
d) Alfred Russell
Explanation: Peter D. Mitchell was the one to propose chemiosmotic hypothesis.
2. ATP synthesis by chemiosmosis is by
a) ATP dehydrogenase
c) ATP synthase
Explanation: ATP synthase synthesizes ATP by chemiosmosis.
3. The measure of potential energy stored as combination of proton and voltage gradients across membrane is termed as
a) Proton motive force
b) Electron motive force
c) Molecule motive force
d) Ion motive force
Explanation: The measure of potential energy stored as combination of proton and voltage gradients across membrane is termed as proton motive force.
4. The transport of acyl co-A for oxidation using a shuttle involves formation of the intermediate
a) Acyl coenzyme A
b) 3 acetyl co-A
c) Acyl cartinine
Explanation: The intermediate acyl cartinine is formed in the transport of acyl co-A for oxidation using a shuttle.
5. Three identical β subunits of the F1 complex during ATP synthesis have
a) Different affinities for ATP and ADP
b) Similar affinities for ADP and ATP
c) Different affinities for ADP but not ATP
d) Different affinities for ATP but not ADP
Explanation: The conformational differences among β subunits extend to differences in their ATP/ADP-binding sites.
6. The acyl co-A formed in the cytosol is transported to
a) Mitochondrial matrix
d) Remains in cytosol
Explanation: The acyl co-A formed in the cytosol is transported to mitochondrial matrix.
7. Every cycle of β-oxidation produces
a) 1 FAD, 1 NAD+ and 2 CO2 molecules
b) 1 FADH2, 1 NADH and 1 acetyl co-A
c) 1 FADH2, 1 NAD+ and 1 acetyl co-A
d) 1 FAD, 1 NADH and 2 CO2 molecules
Explanation: Every cycle of β-oxidation produces 1 FADH2, 1 NADH and 1 acetyl co-A.
8. Membrane potential and proton gradient
a) Cancel one another when uncouplers are present
b) Reinforce one another when respiratory inhibitors are present
c) Are sufficient, separately to make ATP from ADP + Pi
d) Are required to make ATP
Explanation: Membrane potential and proton gradient are both required to make ATP.
9. Long-chain fatty acids are oxidized step-wise in one carbon units starting from
a) Carboxyl end
b) Aliphatic end
c) a and b
Explanation: Long-chain fatty acids are oxidized step-wise in one carbon units starting from carboxyl end.
10. Maximum energy per gram on oxidation is yielded from
Explanation: Fat yields maximum energy per gram on oxidation.
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