Members of the family Flavobacteriaceae exhibit intrinsic multi-drug resistance, which hampers their effective eradication.
Phytochemicals are being explored as alternatives to the use of antimicrobial agents in aquaculture since they have growth-promoting, immunestimulating,
and antimicrobial properties.
Materials and Methods:
The susceptibility of 36 Chryseobacterium
and seven Myroides
spp. isolates from salmon, tilapia and trout as well as 19
selected Flavobacteriaceae type strains to cinnamaldehyde, vanillin and four crude Kigelia africana
extracts (ethyl acetate, dichloromethane,
methanol and hexane), was assessed using disc diffusion assays and compared to standard antimicrobial agents, ampicillin and tetracycline using
Cinnamaldehyde (≥250 μg/ml) was the most effective (77. 8 – 100% susceptibility) while vanillin was the least effective with inhibitory
activity only at 1000 μg/ml. The K. africana
hexane extract (4 mg/ml) was the most effective, with only 11.3% of isolates displaying resistance,
while 94.4% of isolates demonstrated resistance to ampicillin and 38.9% susceptibility to tetracycline. K. africana
extract inhibitory efficacy
decreased in the following order: hexane > ethyl acetate > dichloromethane > methanol. Cinnamaldehyde and K. africana
EX 4 activity indices ≥
1 were obtained for 83.3 - 97.2% and 25% of Chryseobacterium
spp. isolates, respectively, relative to tetracycline.
Cinnamaldehyde and K. africana
fruit hexane extracts are promising candidates to be tested for their efficacy in the treatment of
-associated fish infections. These phytochemicals might serve as environmentally-friendly, cost-effective alternatives
to the use of antimicrobial agents in aquaculture farms, with a lesser chance of resistance development.