A blot, in molecular biology and genetics, is a method of transferring proteins, DNA or RNA, onto a carrier (for example, a nitrocellulosePVDF or nylon membrane). In many instances, this is done after a gel electrophoresis, transferring the molecules from the gel onto the blotting membrane, and other times adding the samples directly onto the membrane. After the blotting, the transferred proteins, DNA or RNA are then visualized by colorant staining (for example, silver staining of proteins), autoradiographic visualization of radioactive labelled molecules (performed before the blot), or specific labelling of some proteins or nucleic acids. The latter is done with antibodies or hybridization probes that bind only to some molecules of the blot and have an enzyme joined to them. After proper washing, this enzymatic activity (and so, the molecules we search in the blot) is visualized by incubation with proper reactive, rendering either a colored deposit on the blot or a chemiluminiscent reaction which is registered by photographic film.
As a first name, Jacques is often phonetically converted to English as Jacob, Jake (from Jacob), or Jack, but the last is not an accurate translation. (Jack, from Jankin, is strictly a diminutive of John.)
People with the surname Jacques
Brian Jacques (1939–2011), British author and radio host, known primarily for the Redwall series
Jaques is one of the main characters in Shakespeare's As You Like It. The 'Melancholy Jaques' as he's always being called is one of Duke Senior's noblemen who lives with him in the Forest of Arden. The role has long been a favorite for famous Shakespearean actors.
Jaques' distinguishing characteristic is his unmitigated cynicism.
He is the only purely contemplative character in Shakespeare. He thinks, and does nothing. His whole occupation is to amuse his mind, and he is totally regardless of his body and his fortunes. Hazlitt describes him as "the prince of philosophical idler; his only passion is thought; he sets no value upon anything but as it serves as food for reflection".
Role in the play
Jaques' role in the play is more of an observer than an actor. Early in the play, Jaques remains a mystery to the audience, being discussed extensively by fellow characters. Once he appears, Jaques' thematic importance and memorable presence in As You Like It is quite impressive.
Jacques is the fifth studio album by the British singer/songwriter Marc Almond. It was released in December 1989. The album is a tribute to the Belgian singer-songwriterJacques Brel and was instigated by Almond's collaboration with Paul Buck, who adapted Brel's original non-English lyrics specifically for Almond.
The songs for the album were recorded at Milo Studios, London, over four years. Almond was accompanied both by his assembled band (comprising "La Magia" and "Willing Sinners" members Annie Hogan, Billy McGee and Steve Humphreys) and various studio musicians. The artwork and cover painting was designed and painted by Johnny Deux.
All songs written and composed by Brel, Buck; except where indicated.