Development of EST-microsatellites from the cycad Cycas rumphii, and their use in the recently endangered Cycas micronesica
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- Cibrián-Jaramillo, A., Marler, T.E., DeSalle, R. et al. Conserv Genet (2008) 9: 1051. doi:10.1007/s10592-007-9447-3
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We developed a set of 12 EST-microsatellite markers (EST-STRs) from Cycas rumphii Miq. and tested them on an ex situ collection of the endangered Cycas micronesica K.D. Hill. The number of alleles per locus in both species ranged from 1 to 15. Observed and expected heterozygosities ranged from 0.000 to 0.842, and 0.000 to 0.883, respectively. All primers amplified in four additional Cycas species. These markers are the first genome-enabled tools for cycad population level studies, and are now available to inform conservation efforts and disentangle the biogeographic history of the genus.