Omics Approaches Towards Systems Biotechnology
Genomic data from high-throughput sequencing are used to assemble de novo the whole genome of Chinese hamster and CHO cells. These cells are important because of their extensive use for the production of recombinant protein therapeutics by the biopharmaceutical industry. The genomic resources for these cells are rather sparse, therefore building genomic information is incredibly useful towards improving bioprocessing. These researchers have studied the transcriptome of these cells to determine diversity of gene expression among different CHO host cells. The quality of the product produced potentially depends on the expression pattern of certain genes in the host cells. The depth of sequencing was also used to identify mutations in CHO cells that potentially enable them to grow in continuously in culture. The researchers used genomic information to verify these mutations. They also used the genomic reads to indentify copy number variants in high-producing CHO cells. These studies are useful in the advancement of bioprocessing of recombinant protein therapeutics.
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