Abstract in English:ABSTRACT: Environmental conditions in broiler houses, specifically temperature, are key factors that should be controlled to ensure appropriate environment for broiler rearing. In countries with tropical/subtropical climate, like Brazil, high temperatures produce heat stress to animals, affecting the production process. This research proposes a real-time model to control temperature inside broiler houses. The controller is a self-correcting model that makes real-time decisions on the ventilation system operation (exhaust fans) together with temperature prediction at the facility. The model involves partial differential equations (PDE) whose parameters are updated according to data registered in real-time. Some experiments were carried out at a pilot farm in the municipality of Jundiaí, São Paulo State, Brazil, for different periods during winter and summer. The results based on simulations in comparison with the current automatic ventilation system show that the model is consistent to keep temperature under control for an efficient production. The model achieved a bias of 0.6 °C on average in comparison with the ideal temperature, whereas the automatic controller measured a bias of 3.3 °C, respectively. Future lines suggest that this approach could be useful in many other situations that involve environmental control for livestock production.
Abstract in English:ABSTRACT: Among soil microorganisms, the genus Cupriavidus has garnered particular scientific, economic and ecological interest because of its ability to fix nitrogen and tolerate high concentrations of metals. The aim of this study was to analyze four strains of Cupriavidus necator for their ability to tolerate and bioaccumulate cadmium and zinc. The tolerance of these strains to these metals was assessed in liquid culture medium containing different concentrations of Zn + Cd and in soil solutions and soils contaminated with multiple elements including Zn, Cd, Cu and Pb. The four strains showed high tolerance to Zn and Cd, both in culture medium and when inoculated into contaminated soil solutions or multi-element contaminated soil. The UFLA02-71 strain displayed the highest ability to bioaccumulate these metals. It was able to accumulate 93.76 µmol g cell−1 of Zn and 16.03 µmol g cell−1 of Cd when cultured in liquid medium with a total heavy metal concentration of 9,140 µmol L−1 (9,000 Zn + 140 Cd) and was able to accumulate 16.98 µmol g cell−1 of Cd in the soil solution. An increase in the pH of the culture medium resulting from the growth of the C. necator strains reduced the Zn2+ and Cd2+ ions in the medium and increased the concentrations of the ZnHPO4 and CdHPO4 species in solution. Thus, we concluded that they show great potential for use in the bioremediation of HM-contaminated areas.
Abstract in English:ABSTRACT: A significant number of bacterial species, particularly in the rhizosphere, may benefit plant growth and development. This group of bacteria is known as plant growth-promoting rhizobacteria (PGPR). This study identified genetically isolates of common bean nodules used to trap bacteria from Amazon pastureland and investigated their capacity of nodulating and promoting growth of common bean when inoculated or co-inoculated with CIAT899 strain (Rhizobium tropici). Two experiments were carried out in a greenhouse, in axenic conditions, using the common bean cultivar Talismã. In the first experiment, 56 PGPR strains were evaluated individually regarding growth promotion and nodulation. In the second experiment, inoculation of seven PGPR strains previously selected in the first experiment was evaluated in three forms of N supply: Co-inoculation with CIAT 899 in the presence of low N-mineral concentration; individual inoculation in the presence of high N-mineral concentration; and individual inoculation in the presence of low N-mineral concentration. The 16S rRNA gene sequencing showed predominance of Pseudomonas genus, identified in 35 % of the sequenced strains. Other genera were identified: Rhizobium, Burkholderia, Xanthomonas and Bacillus. Inoculation of the seven strains with CIAT 899 promoted distinct plant growth in different forms of N supply. In addition, N-mineral supply can be replaced by co-inoculation with strains of Pseudomonas sp. (UFLA 02-281 and UFLA 02-293) and Bacillus sp. (UFLA 02-298) identified in this study.
Abstract in English:ABSTRACT: Longevity is an important trait due to its relationship with profitability. Type traits have been used as indirect predictors for productive life. The objective of this study was to evaluate the relationship of 20 type traits on length of productive life in Brazilian Holsteins, using a piecewise Weibull proportional hazard model. Three analyses were performed i) productive life was corrected for within herd level of production as a proxy for functional longevity, which included the time-dependent effects of region within year, class of milk production within herdyear, milk production class within lactation number, fat class and protein contents within herd and (variation in) herd size as well as the time-independent fixed effect of age at first calving and the type trait score; ii) the effects related to production were omitted from the first model (true longevity) and iii) with the first model, the effect of type was also studied considering five classes of percentage of type-scored cows within the herd. All analyses were performed using the Survival Kit program. The final score, angularity, top line, udder texture and suspensory ligament showed the strongest relationship with productive life. When type traits were available only for a small fraction of the herd, the cows had a better chance of remaining longer in the herd. The absence of type trait phenotypes was associated with a strong increase of culling risk for the cows. Type traits were not found to be good indirect predictors of productive life in Brazil.
Abstract in English:ABSTRACT: Adaptative traits (rectal temperature-RT, respiratory rate-RR) and grazing behavior (Grazing, Ruminating and Rest time, and Sun or Shade time) of Bonsmara-Hereford crossbred-BH, n = 15, and purebred Hereford-HH, n = 18, yearling heifers, in a grazing system of Uruguay. Environment characterization was made using THIadjusted by radiation and wind speed (no, mild, and severe heat weaves), and the comprehensive climatic index-CCI (no, middle, moderate and severe stress). Adaptative traits were measured twice a day, weekly, in two consecutive days in summer and winter at 08h00 and 16h00. Grazing behavior was observed from 07h00 to 21h00 each 600 s twice in summer. The records were analyzed using a mixed model. Significant effect of genotype was observed in mild heat waves for RR and RT in the afternoon (BH lower than HH). For CCI in a.m., the RR was lower than HH in BH in severe stress, while in p.m., the RR was lower in all of CCI levels. RT in p.m. in moderate and severe was lower in BH than in HH. In winter, no differences were found. In grazing behavior, HH rests longer than BH doe; also, HH spends more time in the shade (34 %) than BH does (22 %). BH genotype showed better thermoregulation and grazing behavior at higher temperatures compared to HH.
Abstract in English:ABSTRACT: The chemical composition of corn is variable and the knowledge of its chemical and energetic composition is required for an accurate formulation of the diet. This study aimed to determine the chemical composition, that is, dry matter (DM), mineral matter (MM), neutral detergent fiber (NDF), acid detergent fiber (ADF), ether extract (EE), crude protein (CP), gross energy (GE) and energetic values of different varieties (batches) of corn and validate mathematical models to predict the metabolizable energy values (ME) of corn for pigs using near infrared spectroscopy (NIRS). Corn samples were scanned in the spectrum range between 1,100 and 2,500 nm, the model parameters were estimated by the modified partial least squares (MPLS) method. Ten prediction equations were inserted into the NIRS and used to estimate the ME values. The first degree linear regression models of the estimated ME values in function of the observed ME values were adjusted. The existence of a linear ratio was evaluated by detecting the significance to posterior estimates of the straight line parameters. The values of digestible energy and ME ranged from 3,400 to 3,752 and 3,244 to 3,611 kcal kg−1, respectively. The prediction equations, ME1 = 4334 – 8.1MM + 4.1EE – 3.7NDF; ME2 = 4,194 – 9.2MM + 1.0CP + 4.1EE – 3.5NDF; and ME7 = 16.13 – 9.5NDF + 16EE + (23CP × NDF) – (138MM × NDF) were the most adequate to predict the ME values of corn by using NIRS.
Abstract in English:ABSTRACT: The substantial generation of organic waste together with increasing interest in developing sustainable agriculture heralds an opportunity for mobilizing the recycling of these materials as a source of organic matter and nutrients into a viable management strategy option. A field experiment was conducted to evaluate the use of compost derived from waste produced by the winery and distillery industries in a drip-irrigated melon crop traditionally grown in the area where these wastes are generated. A randomized complete block design was used with four treatments consisting of three different dose levels of compost: 7 (D1), 13 (D2) and 20 (D3) t ha−1 and a control (D0) without the application of compost. The effects of these treatments on plant growth, nitrogen (N) and phosphorus (P) accumulation as well as fruit yield and quality were studied. The application of compost produced a slight increase in plant biomass accompanied by changes in the relative growth rate (RGR) and net assimilation rate (NAR). As a result, a significant improvement in fruit yield was observed in the plots amended with D2, which met all the requirements to obtain elevated yields. In terms of environmental correctness applications were below the limits established by a number of impact indexes. Additionally, the application of compost improved fruit quality resulting in an enhancement of Brix degrees. Although the potential effects of N and P derived from compost were partially masked by other inputs of these nutrients into the system (N in irrigation water, P supplied through fertigation), an effect of P was observed resulting in an increase in the number of individual fruits in the plots amended with compost.
Abstract in English:ABSTRACT: Macrodactylus pumilio Burm. (Coleoptera: Scarabeidae) and Naupactus cervinus (Boh.) (Coleoptera: Curculionidae) are considered primary pests in citrus crops in Brazil, causing damage to plants and decreasing productivity. However, few studies investigate the ecology of these insects. In this context, the use of stable isotopes analysis (SIA) emerges as an alternative technique to conventional studies of behavioral ecology because it is faster and may explain feeding behavior based on the food source for each species. Field sampling and laboratory experiments were carried out to examine the changes of carbon and nitrogen stable isotope ratios (δ13C and δ15N) among pests and host plants (C3 citrus and C4 grasses), providing means to examine trophic interactions. Beetles were collected at the municipality of Gavião Peixoto, São Paulo State, identified and kept at 5 °C in saturated saline solution until the SIA. Two patterns for both species were found: δ13C value for N. cervinus was -23.6 ‰ and -13 ‰ for M. pumilio, indicating similarity between the results of δ13C of N. cervinus and citrus plants (-26 ‰) and dependence on grasses (-12 ‰) for M. pumilio individuals. The mean δ15N value was 4.3 and 5.8 ‰ for citrus plants and grass leaves, respectively, and the mean δ15N value was 4.4 ‰ for N. cervinus and 4.9 ‰ for M. pumilio. The results showed a higher affinity of N. cervinus for citrus roots since the larval stage compared with the alternative diet on M. pumilio.
Abstract in English:ABSTRACT: The phenotypic and genetic analysis of apple (Malus × domestica Borkh) genotypes is essential for breeding species. Information on the morphology and genetic structure of apple offers significant help for germplasm maintenance and selection of suitable material to breed superior cultivars. This study shows the results of an investigation on the morphology and the genetic diversity for 19 apple cultivars, which are preserved in an ex situ collection in Naoussa, Central Macedonia, Greece. Information was recorded over a 5-year period for 47 traits describing plant morphology and phenotype, as well as leaf and fruit quality. Data were analyzed using the principal component analysis (PCA) method. The Euclidean distance metric and the Ward’s agglomeration method were used in an unsupervised hierarchical cluster analysis of all cultivars. The cultivars were grouped into four main clusters, suggesting that the characterized apple collection has a high potential for specific breeding goals. Furthermore, the cultivars were genotyped using seven microsatellite primers. Moderate levels of polymorphism were detected, and 38 distinctive alleles (5.4 alleles per primer pair) were identified. Both multivariate clustering approach (phenotypic data) and the genetic distance clustering approach (genetic data) grouped the apple cultivars according to their type. Hence, these data could be used for protection or patenting processes of existing or new apple cultivars carried out by the EU-Community Plant Variety Office.
Abstract in English:ABSTRACT: The demand for effective insecticides in pest control with low toxicity to the non-target organisms, such as natural enemies and pollinators, is increasing steadily. A good alternative for synthetic insecticides is natural compounds, including essential oils (EO). This work assessed toxicity of essential oils extracted from Artemisia annua, A. absinthium, A. camphorata, A. dracunculus and A. vulgaris against the melonworm Diaphania hyalinata (Linnaeus, 1758) (Lepidoptera: Crambidae) larvae, a pest of Cucurbitaceae, and their selectivity for fire ant Solenopsis saevissima (Smith) (Hymenoptera: Formicidae) and jataí bee Tetragonisca angustula (Latreille) (Meliponinae). The plants were grown in a greenhouse with mineral fertilization and were used for EO extraction. The insects in the bioassay belonged to the second instar of D. hyalinata and adult forms of S. saevissima and T. angustula. Essential oil from A. annua induced a high mortality rate in D. hyalinata (96 %) over a 48 h period. The same essential oil was selective for predator S. saevissima (42 % mortality) and pollinator T. angustula (74 % mortality), while causing high mortality in D. hyalinata. The insecticidal activity of A. annua oil was attributed to the synergism of its constituents viz., camphor and 1,8-cineole. Therefore, this essential oil contains constituents that are promising for effective use as insecticide due to its high toxicity and rapid action against D. hyalinata as well as low toxicity for predator and pollinator.
Abstract in English:ABSTRACT: Mechanical harvesting leaves in its wake a considerable amount of straw in the field, which can be effectively utilized to improve the soil condition and sugarcane yield. However, there is no specific information as to the quantity of straw mulch required to achieve such effects and as to whether it can be used in other sectors for bioelectricity and ethanol production. The aim of this research was to evaluate the effect of removing different amounts of straw from the field and its impact on the yield and industrial quality of sugarcane ratoons. The experiment was carried out on Rhodic soil where six treatments were evaluated including 0 %, 25 % (5 Mg ha−1), 50 % (10 Mg ha−1), 75 % (15 Mg ha−1), 100 % (20 Mg ha−1) straw on the soil surface and burned sugarcane (where 100 % of the straw was burned). The influence on yield and industrial quality was calculated using total soluble solids, Pol (Apparent sucrose content), apparent purity, total sugars, reducing sugars and fiber. Shifting the harvesting system from burned cane to growing under straw mulch improved crop yield as well as favoring sugar contents during water deficit conditions. The straw left on the soil did not affect industrial quality in any way during the trials; however, under drought conditions, treatments with 50 and 75 % of straw resulted in a 76 % higher yield compared to burned sugarcane, and 29 % more than the 0 %, 25 % to 100 % treatments of straw mulch thus favoring higher sugar production. The removal of 50 % of the straw caused no damage to the sugarcane crop.