Plot v/[A] versus v for the following cases:
a. There is a total of ten identical and independent sites per polymer and the intrinsic binding constant is K = 5 × 105 M-1
b. There is a total of ten independent sites per polymer. Nine of the ten sites are identical, with 5.0 × 105 M-1.The binding constant for the tenth site is 5.0 × 106 M-1. For more than one type of site, the Scatchard equation can be generalized to
[Note that the deviation from linearity is small for part (b). Because experimental error in such measurements is usually appreciable, be cautious in concluding from a linear Scatchard plot that the sites are identical.]
c. Repeat the calculation and plot of part (b) for five identical sites of each type.