Jan. 11, 2011
CU scientists advance research of lethal Listeria
Listeria is an opportunistic pathogen that causes brain infection, blood poisoning, abortion and death for about 250 Americans and a number of farm animals each year. But while its harmful strains can be more lethal than Salmonella, it exists in benign species and strains as well.
By finding out why some forms are fatal and others are not, Cornell researchers are developing more effective ways to detect and prevent food-borne illness.
When it comes to predicting whether strains of the bacterium will be harmful, Martin Wiedmann, associate professor of food science, has found that it is necessary to test for the presence of several genes, rather than just one.
Even among Listeria monocytogenes, which causes the vast majority of human disease cases, his team identified a strain that lacked one gene linked to the ability to cause disease.
In a paper published Dec. 2 in the journal BMC Genomics, Wiedmann also describes how the genus Listeria has evolved over the past 47 million years.
Unlike some other bacteria, Listeria strains actually appear to have become less virulent over time. While their common ancestor carried all genes needed to cause disease, many of the strains that emerged did not, and only two of the six Listeria species Wiedmann studied were found to have all the genes likely needed to cause human disease.
This may be because it is in the best interest of the bacteria that their hosts are kept alive, he said.
Originating in soil and often traveling to animals through plant materials, Listeria bacteria thrive inside mammalian bodies, where there is limited competition for food. They can invade human cells and are able to move from one cell to the next, avoiding the antibodies we have floating around our bodies, Wiedmann said.
Listeria monocytogenes causes listeriosis, a rare but potentially lethal food-borne infection, with symptoms similar to meningitis. About 20 percent of people diagnosed with listeriosis die, compared to less than 1 percent of those inflicted by Salmonella.
Listeria has been found in animal silage, raw meats and vegetables, unpasteurized milk and different processed foods. Pasteurization and sufficient cooking typically kill Listeria, but contamination can occur after heating and before packaging, so extensive sanitation is required at processing plants, Wiedmann said.
"It has a canny ability to survive very, very well," Wiedmann said. "In one case, we found a strain that had survived in a processing plant for 12 years."
Wiedmann's team has helped control previous Listeria outbreaks by tracing the origin of contamination, thanks in part to a Web-based pathogen tracker database they also developed. He also recently identified a unique strain of Listeria monocytogenes that was responsible for an outbreak among dairy cattle, with his findings detailed in a paper to be published in the Journal of Veterinary Diagnostic Investigation.
"We create the basic knowledge, which industry can then develop into practical applications," Wiedmann said.
Industry has already taken notice. Study collaborator Life Technologies Corp. is planning to use the information Wiedmann collected to develop assays that specifically detect pathogenic Listeria strains, according to bioinformatics scientist Craig Cummings.
His company developed The SOLiD System, an advanced, "next generation" sequencing instrument used in the study.
"This system allows us to discover the differences between large sets of bacterial strains in a relatively fast and affordable way," Cummings said.
Wiedmann's paper is the first published documentation of the equipment's use in this context and will likely establish a protocol for doing similar studies for other groups of pathogens, Cummings added.
Stacey Shackford is a staff writer at the College of Agriculture and Life Sciences.