|Reference : Cyanobacterial molecular diversity and distribution in microbial mats from ant...|
|Scientific congresses and symposiums : Poster|
|Life sciences : Microbiology|
|Cyanobacterial molecular diversity and distribution in microbial mats from antarctic lakes|
|De Carvalho Maalouf, Pedro [Université de Liège - ULg > > Centre d'ingénierie des protéines >]|
|Lambion, Alexandre [Université de Liège - ULg > Département des sciences de la vie > Enzymologie >]|
|Zakhia, Frédéric [> >]|
|Wilmotte, Annick [Université de Liège - ULg > Département des sciences de la vie > Physiologie et génétique bactériennes >]|
|Analyzing complex microbial communities and their host microbe interactions|
|Belgian society for microbiology|
|[en] cyanobacteria ; biogeography ; antarctica ; molecular diversity ; DGGE|
|[en] The coastal deglaciated areas of Antarctica hold lakes and other water bodies with a wide spectrum of limnological conditions. Aquatic habitats offer milder conditions to the microorganisms, which are the only permanent inhabitants of this glacial desert. Among them, cyanobacteria are the first photosynthetic colonisers, sometimes forming thick, cohesive and pigmented benthic mats.
In the frame of the BELSPO AMBIO project (Antarctic Microbial BIOdiversity, influence of geographical and ecological factors, www.ambio.ulg.ac.be), we have obtained benthic mat samples coming from lakes from various coastal regions of the continent (MERLIN 2007 and BELARE 2007 field campaigns and by collaborations).
We have investigated cyanobacterial molecular diversity via Polymerase Chain Reaction and Denaturating Gradient Gel Electrophoresis (PCR-DGGE) on a set of 80 samples. We couple the numerical analysis of the obtained band patterns with the phylogenetic analysis of the sequences, and by using multivariate analysis, we will assess the role of the ecological and geographical factors shaping the distribution and the diversity of cyanobacteria.
Preliminary results of the analysis of 13 samples seem to indicate that lakes separated by a small distance have different cyanobacterial communities, highlighting the importance of ecological factors. Fifty per cent of the obtained Operational Taxonomic Units (OTUs) are potentially endemic to Antarctica while others seem to have a global distribution.
The completed study will give us a wide scale view on the distribution and the diversity of cyanobacteria in two biogeographical zones: Continental Antarctica and Maritime Antarctica. Finally, our studies will supply new data and arguments for the ongoing “everything is everywhere, but the environment selects” debate concerning microbial biogeography.
|Centre d'Ingénierie des Protéines - CIP|
|Antarctic Microbial Biodiversity : the importance of geographical and ecological factors|
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