After blotting, the target protein will be detected using appropriately matched and labeled antibodies . The typical immunodetection stage involves a few basic steps:
Figure 10: Immunodetection Overview. The Western blot is blocked, incubated with antibodies, and treated with substrate to make the target protein visible . Wash steps are carried out between incubations to remove excess unbound material and to minimize non-specific signal on the immunoblot.
|Blotting||Immunodetection: Blocking and Antibody Incubation|