Metopism, which is defined as a condition in which the two pieces of the frontal bone fail to merge in early childhood, displays varying degrees of incidence. In this study, the variation of the frequency of metopism across historical periods is investigated on the skulls of 487 adults from 12 different Ancient Anatolian populations dated to various periods of history ranging from the Neolithic to the first quarter of the 20th century. In addition, the study also examines the relationship of metopism to sex and cranial form. It is revealed that the frequency of metopism showed a relative increase across time periods in Anatolia after the Neolithic Period, with the exception of the Cevizcioglu Ciftligi population. However, no significant relationship was found between metopism and cranial form or sex. It is found that the frequency of metopism in Ancient Anatolia had a distribution range of 3.3-14.9%. This distribution shows that the inhabitants of Anatolia have a heterogeneous genetic make-up due to the geographical situation of Anatolia, which has been open to gene flow both in the past and at present.