How does real-time PCR system work? In order to understand the difference between real-time PCR and traditional PCR, it’s important you first know what it is. Real-time PCR is a system that uses computer technology to pinpoint the exact location at which there may have been an error in your DNA sequence. It’s a lot faster than traditional PCR, but because of its reliance on technology, it is more expensive.
How Does Traditional PCR system and real-time PCR system work?
Traditional PCR is a method that amplifies DNA sequences in real-time. This means that the reaction proceeds rapidly and the result can be seen immediately. Real-time PCR is often used for detecting very specific DNA sequences.
The Traditional PCR system uses two sets of primers, one to amplify the desired DNA sequence and one to check for background noise. The speed of traditional PCR is determined by how long it takes for the primers to hybridize to the target DNA molecule and for the polymerase to copy the sequence. As this process repeats, the amount of amplified DNA in the sample increases.
Real-time PCR system is a technique that uses PCR to detect a specific sequence of DNA in a sample. By doing this, real-time PCR can be used to identify specific genes or sequences of DNA.
What Is the Difference Between Traditional PCR and Real-Time PCR?
Traditional PCR is a method of DNA sequencing that has been used for years to sequence large amounts of DNA. The traditional PCR process involves taking long, sequential steps to amplify the target DNA molecule. One of the limitations of traditional PCR is that it can take quite a while to complete a sequencing reaction, which can be problematic if you’re trying to sequence a very short strand of DNA.
Real-time PCR, on the other hand, is a more recent technology that allows you to sequence DNA in real-time. With real-time PCR, you immediately detect the presence of specific DNA sequences by measuring the amount of amplified product that is produced during the sequencing reaction. This approach eliminates the time lag associated with traditional PCR and makes it easier to sequence short stretches of DNA.
Ultimately, there are advantages and disadvantages to both traditional and real-time PCR methods. If you’re looking to sequence a lot of DNA quickly and accurately, traditional PCR is likely your best option. If you only need to sequence short stretches of DNA frequently, though, real-time PCR system from Sansure may be a better choice.