Intake of total flavonoids (mg/day) in different countries/regions.
Dietary patterns and food availability differ greatly between regions and countries around the world. As a result, there is a large variability in the intake of total flavonoids and flavonoid subclasses, and subsequently in their major food sources. However, we need to be aware of certain methodological issues when we compare studies on flavonoid intake.
- food sources
Flavonoids are ubiquitously distributed plant secondary metabolites, particularly in fruits, vegetables, legumes, nuts, chocolate, and derived beverages (e.g., tea, wine, and juices) . They are synthesized through the phenylpropanoid pathway, converting phenylalanine into 4‐coumaroyl‐CoA, which then enter the flavonoid biosynthesis pathway. Finally, various enzymes modify the basic flavonoid skeleton, leading to the different flavonoid subclasses . In plants, flavonoids fulfill many different functions, such as protecting against ultraviolet radiation and phytopathogens, and acting as pigments, chemical messengers, physiological regulators, and cell cycle inhibitors .
Over the last three decades, flavonoids have received a lot of attention in cellular and animal models due to their well‐established biological properties such as antioxidant, anti‐inflammatory, and anti‐carcinogenic effects, especially inducing enzymes and modulating metabolic and cell signaling pathways . However, the epidemiologic evidence on the reduction in the chronic disease risk is still limited and usually inconsistent [4, 5]. The strongest evidence of their health‐protective effects is for cardiovascular diseases  and type 2 diabetes . One of the potential explanations of these inconsistent results is the difficulty in accurately assessing dietary flavonoid intake and the large variability of flavonoid intake among populations/countries.
This chapter is focused on the large differences in dietary flavonoid intakes and food sources worldwide, but prior to this, it is also important to briefly summarize the complexity of flavonoid chemistry and classification, and the different possible methodologies to assess dietary flavonoid intake.
2. Chemistry and classification of flavonoids
Flavonoids are a group of natural compounds with variable phenolic structures. In 1930, a new substance was isolated from oranges. At that time, it was believed to be a member of a new class of vitamins and was named as vitamin P. Posteriorly, it appeared that this compound was the flavonoid rutin and since then more than 9000 varieties of flavonoids have been identified .
Chemically, flavonoids are formed by a C6–C3–C6 structure, which consists of two benzene rings (A and B) linked by a three‐carbon chain that form an oxygenated heterocycle (C ring) (Figure 1). They can be divided into nine classes according to their chemical structures: flavanones, flavones, dihydroflavonols, flavonols, flavan‐3‐ols, or flavanols [including monomers, proanthocyanidins, and flavanol‐derived compounds (theaflavins and thearubigins)], anthocyanins, isoflavones, chalcones, and dihydrochalcones (Figure 2) . The various classes of flavonoids differ in the level of oxidation and pattern of substitution of the C ring, while individual compounds within a class differ in the pattern of substitution of the A and B rings. Flavonoids in nature occur mostly as glycosides, aglycones (especially flavanols), and, in few cases, as methylated derivatives . The basic flavonoid structure is the aglycone. When glycosides are formed, the glycosidic linkage is normally located in positions 3 or 7 and the sugar can be L‐rhamnose, D‐glucose, glucorhamnose, galactose, or arabinose . This is very relevant because bioavailability differs among flavonoids, depending on the aglycone, the type of monosaccharide attached, and its position .
3. Dietary assessment of flavonoids
The most common method of estimating flavonoid intake in epidemiologic studies is to use dietary questionnaires, such as food frequency questionnaires (FFQ), 24‐h dietary recalls, and food diaries, to record all food consumption over a known period of time dietary surveys, especially FFQ, are prone to several limitations, particularly regarding the identification of specific foods (e.g., the large variety of products available on the market, different food processing and cooking techniques, distinct ingredients, and preparation of recipes) and accurate quantification of portion sizes . Despite this, FFQs are the most common method used in large epidemiological studies, mainly because they are an easy, quick, and an economical way to record the participants’ habitual diet. In order to improve both quantification and quality of the data gathered, the use of a previously validated FFQ on flavonoids or flavonoid‐rich foods is essential. Moreover, the use of innovative technologies and methodologies for the dietary assessment of flavonoid intake such as the collection of multiple 24‐HDRs and food records and interactive computer‐ and camera‐based technologies will certainly improve this process .
In order to estimate the intake of flavonoids, a food composition table or database is also needed. There are currently two main databases. The first one is the US Department of Agriculture (USDA) database on flavonoids, isoflavones, and proanthocyanidins, which was created in 2003, 1999, and 2004, respectively [13–15]. Since then, several updates have been released. The USDA databases contain worldwide food composition data on the six main flavonoid classes (flavanols, flavonols, flavanones, flavones, isoflavones, and anthocyanidins) expressed as aglycones. Phenol‐Explorer is the second most common database . It was developed in 2009 and contains worldwide data, but in this case, on all nine flavonoid classes (including chalcones, dihydrochalcones, and dihydroflavonols) expressed as they are found in nature (mainly as glycosides) and analyzed with chromatography without hydrolysis. Phenol‐Explorer also contains data on chromatography after hydrolysis and, in this case, flavonoids are expressed as aglycones, but these are not usually used. In the studies using Phenol‐Explorer using flavonoid data expressed as aglycone equivalents, flavonoid glycoside contents are converted into aglycone contents using their respective molecular weights [17, 18]. The other important difference between databases is that Phenol‐Explorer does not contain data on thearubigins because the composition data quality on thearubigins is very low . The non‐specific spectrophotometric method used to date only provides a crude estimation of their concentrations in black teas, which are the only known food sources of thearubigins.
These differences between databases (classes of flavonoids, aglycones vs. glycosides, and inclusion of thearubigins) complicate the comparison among studies using different databases. In this book chapter, we have mostly compared studies using the USDA databases or Phenol‐Explorer with data expressed as aglycones. In our comparisons, we have also made distinctions between the studies including proanthocyanidins and thearubigins, which are the most controversial flavonoid compounds.
4. Geographical differences in total flavonoid intake and their food sources
The intake of flavonoids varies greatly by country/geographical region due to different dietary patterns; although differences in the flavonoid classes included, the dietary assessment methods, the food composition database use, and the method of expression (glycoses vs aglycones) applied complicate these comparisons. Table 1 summarizes the most important descriptive studies assessing flavonoid intakes in different countries/regions. The mean intake of flavonoids worldwide ranges between 150 and 600 mg/day expressed as aglycones without thearubigins [20–26]. Taking into account thearubigins, the flavonoid intake could increase by a negligible amount in populations with low black tea intake, but would double the intake in black tea‐consuming countries, such as the UK (from ∼500 to >1000 mg/day) .
|Study||Country||Population||Dietary assessment||FCDB||Aglycones vs. glycosides||Total flavonoid intake (mg/day)||Major class contributor||Main food sources||References|
|Calcium Intake Fracture Outcome Age‐Related Extension Study||Australia||1136||SemiQ‐FFQ||USDA||Aglycones||696||Flavanols**||Black tea|||
|The Blue Mountains Eye||Australia||79||3 4‐WFR||USDA||Aglycones||683||Flavanols**||Black tea|||
|SUVIMAX||France||4942||6 24‐HDR||PE||Aglycones||436*||PA||Fruit, tea, red wine|||
|FINDIET 2002||Finland||2007||24‐HDR||Own database||Aglycones||209*||PA||Berries, fruit|||
|Polish National Multicenter Health Survey||Poland||6661||24‐HDR||USDA, PE||Aglycones||524.6||Flavanols**||Tea, apples|||
|NHS I & NHS II & HPFS||US||156,957||FFQ||USDA||Aglycones||391||PA||Tea|||
|Mexican Teachers’ Cohort||Mexico||106,466||FFQ||PE||Aglycones||140*||PA||Fruit and orange juice|||
|Health Survey‐São Paulo||Brazil||1103||24‐HDR||PE||Glycosides||54.6*||Flavanones||Citrus fruit, beans|||
|Cross‐sectional study||China||3317||SemiQ‐FFQ||USDA||Aglycones||225*||Flavanols**||Soy, pome fruit|||
|KNHANES||Korea||33,581||24‐HDR||USDA, PE, own database||Aglycones||318||PA||Fruit, tofu, onions|||
|Tehran Lipid and Glucose Study||Iran||2618||FFQ||USDA||Aglycones||1652||Flavanols**||Vegetables, fruit|||
Europe is the continent with the most number of studies assessing the intake of total flavonoids, and therefore, it is possible to describe their intake quite accurately. Moreover, studies like the European Prospective Investigation into Cancer and Nutrition (EPIC) study allow us to compare the intake in 10 European countries using the same methodology [21, 26]. In Europe, an increasing south‐to‐north gradient is usually observed . Despite the high intake of fruits, vegetables, and red wine in Mediterranean (MED) countries, the intake of total flavonoids in these countries (250–400 mg/day) is lower than in non‐MED countries (350–600 mg/day), even without taking into account thearubigins. This is due to the much higher consumption of tea in non‐MED compared to MED countries. These differences would be larger if thearubigins were also included . The highest total flavonoid intake in Europe is in the UK, due to the traditional tea culture [21, 26]. Eastern European countries, such as Poland, also have a high intake of total flavonoids (∼600 mg/day), related to their high tea consumption [32, 40]. Southern regions of France are considered as a MED region, while northern regions as a non‐MED region; therefore, France has an intermediate intake of total flavonoids, with the main food sources being fruit, tea, and red wine (a combination of food sources from MED and non‐MED countries) . In Scandinavian countries with a low consumption of tea, such as Finland, the intake of total flavonoids (200–250 mg/day) is lower than MED countries, since the consumption of fruit is lower as well .
In Australia, there are various studies describing the intake of total flavonoids [20, 28, 29]. As a high tea‐consuming country, Australia has a high intake of total flavonoids (650–700 mg/day), including thearubigins. In the three Australian populations documented, black tea contributes to at least 75% of total polyphenols.
In America, several studies have estimated the intake of total flavonoids, especially in the US [22, 34–36, 41], since the USDA databases were developed there [13–15]. In the US, the mean intake of total flavonoids varies from 250 to 400 mg/day, including proanthocyanidins and thearubigins [22, 34–36, 41]. Although the consumption of tea is not very high, tea is still the main food source of total flavonoids in the US, probably due to a low consumption of fruits and vegetables. In the US, some differences in the total flavonoid intake among ethnicities have been observed [22, 36]. Non‐Hispanic whites have the highest intake of flavonoids (>300 mg/day), whereas non‐Hispanic blacks, Mexican Americans, and other ethnicities consume approximately 200 mg/day. Data on other countries in America is very limited, and to the best of our knowledge, Brazil  and Mexico  are the only other countries with available data. The intake of total flavonoids is around 150 and 50 mg/day in Mexico and Brazil, respectively. These are the countries with the lowest intake of total flavonoids that have been published worldwide. Although, the studies in these countries did not take thearubigins into account, the contribution of thearubigins to total flavonoids is insignificant because Brazilians and Mexicans rarely drink tea. The main food source of total flavonoids is citrus juices, followed by fruit in Mexico and beans in Brazil [23, 37]. In these Latin‐American countries, a potential relevant underestimation of total flavonoids cannot be ruled out because of missing food composition data on some tropical foods, such as fruits and vegetables (e.g., mamey, zapote, papaya, sweet potato, nopal, guava, jicama, and prickly pears) that are frequently consumed in their diets and may be good sources of flavonoids.
In Asian countries, very little complete data are available, as there is only data for East Asian countries (such as China [24, 38] and South Korea ). The total flavonoid intake in China ranges from 65 mg/day without proanthocyanidins  to 225 mg/day with proanthocyanidins . In both studies, thearubigins are not included, but Chinese people drink green tea, but not black tea. Thearubigins are formed during the fermentation of green tea to black tea. In South Korea, the intake of total flavonoids is slightly higher than in China (320 mg/day) . In East Asian countries, soy and its derived products (the main food sources of isoflavones) are one of the most important contributors to total flavonoids, although proanthocyanidins and flavan‐3‐ol monomers are the most abundant flavonoids in South Korea and China, respectively. In Japan, China, and South Korea, there are many studies focusing on isoflavones, due to their potential phytoestrogenic effects , but not on total flavonoids. In the Middle East, a recent study in Iran has been conducted showing a mean intake of 1650 mg/day . This is the highest mean of total flavonoid intake worldwide. This high amount is because of the elevated consumption of black tea in these Middle East populations.
In summary, there is a high heterogeneity between countries in total flavonoid intake. The highest intake is in Iran , followed by the UK (>1000 mg/day)  and the lowest intake is in Brazil and Mexico (<150 mg/day) [23, 37]. The populations with a higher intake of total flavonoids are those with a high consumption of tea, especially black tea. In this case, the main contributor to total flavonoids is thearubigins, and this may be partially explained due to the inaccurate measurement of thearubigin content in black tea . Populations with a lower intake of total flavonoids are those with a low consumption of tea. In these cases, the main food sources are fruit and proanthocyanidins become the main contributor to total flavonoids. In East Asia, isoflavones are also important contributors to total flavonoids due to the high consumption of soy‐derived products. Further studies are warranted to estimate the intake of total flavonoids in other regions of the world with little available data, such as Latin America, Africa, and Middle East. More research on the content of flavonoids in food is also needed in order to improve the existent food composition data, adding new foods (e.g., tropical foods) and refining the thearubigin data.
5. Determinants of the total flavonoid intake
Total flavonoids, as with most nutrients and dietary compounds, are positively correlated to total energy intake. Therefore, subjects consuming more energy are also more likely to be those with a higher intake of total flavonoids, even though one of the most relevant food sources is tea which is a non‐caloric beverage. For this reason, if we want to study the determinants of total flavonoid intake, we should adjust our models for total energy intake.
Men usually consume higher amounts of total flavonoids, but after adjusting for total energy, women actually tend to consume more total flavonoids, as reported in the US  and South Korean populations . However, men have a higher flavonoid intake in MED countries  and France  as men tend to drink more wine, particularly red wine. In contrast, the opposite was found in non‐MED countries because women tend to consume more tea than men .
Young adults are more likely to consume less total flavonoids than older adults [21–23, 33]. The age range with the highest intake of total flavonoids is between 55 and 70 years, probably because they consume a more traditional dietary pattern and therefore more fruit, vegetables, red wine, and tea (depending on the region). On the other hand younger adults (20–40 years) tend to follow a more Westernized diet, with less plant‐based foods. Although total flavonoid intake is usually higher in the older age groups, subjects are likely to keep their dietary habits throughout adulthood. Therefore, no significant differences have been found in the estimation of total flavonoid intake after several years of follow‐up [22, 41].
Fruit, tea, red wine, and vegetables are the most important food sources of total flavonoids [21, 26]. These plant‐based foods are characteristic of healthy and traditional diets, and therefore, subjects with high adherence to healthy dietary and lifestyle habits are more likely to have a higher intake of total flavonoids. For example, individuals with a normal weight (BMI < 25), a high education level, and a high socioeconomic status and who are physically active and non‐smokers (never and former smokers) tend to have a higher total flavonoid intake [22, 23, 26, 33].
6. Flavanols: intake and food sources
Total flavanols or flavan‐3‐ols is the most consumed flavonoid class by far worldwide (Table 1), contributing to >80% of total flavonoids. Flavanols are divided into three subclasses: flavan‐3‐ol monomers (including catechins and epicatechins), proanthocyanidins (including oligomers and polymers of flavanols), and flavanol‐derived compounds (including thearubigins and theaflavins). In nature, flavanols are predominantly found as aglycones.
The intake of total flavanols ranges from 11 mg/day in Brazil  to 629 mg/day in Australia  (Table 2). These large differences among populations are due to different intakes of the main food sources (i.e., tea and fruit). Depending on the intake of tea (a beverage rich in flavan‐3‐ol monomers and derived compounds) and fruit (foods rich in proanthocyanidins) a rank of countries/regions can be proposed: (i) countries with a very high consumption of tea (such as Australia, UK, and Poland), which have a mean intake between 450 and 600 mg/day [28, 29, 33, 43, 44]; (ii) countries with a moderate consumption of tea or a very high consumption of fruit (such as MED countries, Germany, and the US), which have a mean intake of 250–400 mg/day [17, 35, 43, 45]; (iii) countries with a low consumption of tea and low‐moderate consumption of fruit (such as Scandinavian countries, South Korea, and Latin‐American countries), with a mean intake ranging from 50 to 150 mg/day [23, 25, 31, 37]. In Latin‐American countries, an underestimation of the total flavanol intake is probable, since there is little food composition data available on tropical foods, especially on fruit [13–16].
|Study name||Country||N||Dietary assessment||FCDB||Glycosides vs. aglycones||Anthocyanidins||Flavones||Flavonols||Flavanones||Total Flavanols||Isoflavones||Chalcones||Dihydrochalcones||Dihydroflavonols||References|
|Total||Flavan‐3‐ol monomers||PA||Flavanol‐derived compounds|
|Calcium Intake Fracture Outcome Age Related Extension Study||Australia||1136||SemiQ‐FFQ||USDA||Aglycones||28.0||56.0||570|||
|Longitudinal Assessment of Ageing in Women||Australia||511||Diet history||USDA||Aglycones||4.1|||
|The Blue Mountains Eye||Australia||79||3 4‐d WFR||USDA||Aglycones||7.0||1.9||28.7||21.2||629|||
|Membership database of Flemish Dietetic Association||Belgium||45||SemiQ‐FFQ & 4DFR||based on USDA||Aglycones||6.9||4.3||19.6||37.7||107|||
|EPIC||MED||36,037||24HDR||USDA, PE||Aglycones||37.4||5.6||24.8||33.7||282||48.5||218.4||15.0||1.5||13.5||0.4||[40, 43, 48–50]|
|Non‐MED||USDA, PE||Aglycones||27.3||4.1||29.5||31.8||430||93.1||180.1||157.1||7.1||150.0||0.7||[40, 43, 48–50]|
|EFSA database||Northern Europe||30,000||FFQ & 24HDR||USDA, PE||Aglycones||241||58.8||160.0||113.2||7.9||105.3|||
|Austrian institutionalized elderly population||Spain||304||FFQ||PE||Glycosides|||
|Case‐control study in Italy||Italy||4154||FFQ||USDA||Aglycones||20.0||0.5||21.6||38.3||325||57.4||267.0||0.03||[53, 54]|
|Case‐control study in Greece||Greece||200||SemiQ‐FFQ||USDA||Aglycones||12.2||3.0||9.6||58.1||10.9||0.8|||
|SU.VI.MAX study||France||4942||6 24HDR||PE||Aglycones||35.0||18.0||34.0||13.0||87.0||227.0||9.0||5|||
|German National Nutrition Survey II||Germany||15,371||Diet history||USDA, PE||Aglycones||386||119.8||196.4||69.7|||
|Polish National Multicenter Health Survey||Poland||6661||24HDR||USDA||Aglycones||20.9||0.6||32.0||9.2||462||0.2|||
|Leeds Women's Wellbeing Study (LWW), the Diet and Health Study (DH)||UK||246||3‐ 7d FR||USDA, PE||Aglycones||19.1||2.8||58.3||21.4||506||2.4||1.9|||
|Case‐control study in Scotland||Scotland||1456||SemiQ‐FFQ||Own database||Aglycones||1.0||28.0||20.6||115.2||33.5|||
|FINDIET 2002 Study||Finland||2007||24‐HDR||Own database||Aglycones||47.0||5.4||27.0||128||12.0||20.0||0.9|||
|NIH‐AARP Diet and Health Study||US||491,840||FFQ||USDA||Aglycones||12.7||1.2||19.1||37.1||101.9||0.6|||
|NHS I & NHS II & HPFS||US||156,957||FFQ||USDA||Aglycones||13.9||1.8||18.4||41.0||307||55.6||251.3|||
|NHANES||US||8809||24HDR, FFQ||USDA||Aglycones||3.0||1.5||12.9||14.4||230.6||74.1||1.1||[20, 36]|
|Women's Health Initiative||US||96||FFQ||USDA||Aglycones||2.1|||
|Mexican Teachers’ Cohort||Mexico||106||SemiQ‐FFQ||PE||Glycosides||27.0||1.0||12.9||60.2||121||17.4||103.9||0.0||0.0||0.0||2.1||<0.01||1.4||<0.01|||
|Mexican case‐control study||Mexico||478||FFQ||USDA||Aglycones||6.6||35.6||6.9|||
|Cohort study in the state of Morelos||México||50||FFQ||USDA||Aglycones||7.5||29.3||10.0|||
|Health Survey‐São Paulo (ISA‐Capital 2008)||Brazil||1103||24HDR||PE||Glycosides||6.8||3.6||14.6||16.1||11||1.5|||
|Health conditions, nutrition, and use of medication by the elderly in Viçosa (Minas Gerais)||Brazil||620||Recall of habitual consumption||PE||Aglycones||2.2||4.7||23.7||36.4||377||1|||
|Cross‐sectional Chinese study||China||3317||SemiQ‐FFQ||USDA||Aglycones||5.3||0.7||14.0||5.8||123.9||48.7||6.3|||
|Shanghai Breast Cancer Study||China||1823||FFQ||USDA||Aglycones||68.6|||
|Case‐control study in Shanghai||China||1393||FFQ||Own database||Aglycones||27.4||10.4||123.8||3.54|||
|Study in Suihua, northern China||China||887||FFQ||Own database||Aglycones||29.2||32.6|||
|Case‐control in China||China||560||FFQ||Own database||Aglycones||29.2|||
|Case‐control in the northeast of China||China||66||FFQ||USDA||Aglycones||17.2||2.1||22.8||5.1||13.3|||
|Case‐control in Japan||Japan||340||FFQ||Own database||Aglycones||39.6|||
6.1. Flavan‐3‐ol monomers
Intake of flavan‐3‐ol monomers varies between 10 mg/day in Mexico [23, 58, 59] and 270 mg/day in Poland , contributing to approximately 15–30% of total flavanols. The main food sources of flavan‐3‐ol monomers are tea, fruit (e.g., apples, stone fruits), red wine, and cocoa products . The intake of these foods and beverages will determine the levels of flavan‐3‐ol monomers in the diet. Countries with a high intake of tea have a major intake of these compounds, such as Australia, UK, and Poland (150–250 mg/day), followed by countries with a high consumption of fruit and red wine, such MED countries and France (50–100 mg/day), and finally countries with a low consumption of tea and certain types of fruit, such as Latin American and Finland (<l50;mg/day) (Table 2).
Proanthocyanidins account for >75% of total flavanols in countries with a low intake of tea (MED countries) and <40% of total flavanols in countries with a high intake of tea (non‐MED countries) [40, 43]. Proanthocyanidins are characteristic flavonoids in fruit, particularly apples, stone fruits, and berries, as well as red wine, but not in tea . The highest intake of proanthocyanidins is in MED countries, including France (>200 mg/day) [17, 43, 53], followed by non‐MED countries and the US (150–200 mg/day) [32, 35, 36, 43, 45], and finally Latin‐American and Asian countries (<150 mg/day) [23–25] (Table 2).
6.3. Flavanol‐derived compounds
Theaflavins and thearubigins occur exclusively in tea . Theaflavins are consumed in low amounts (<3 mg/day) in countries with low tea consumption and larger amounts (10–30 mg/day) in countries with an important tea culture (Table 2) . The same pattern can be applied to thearubigins, which can only be found in black tea. The intake ranges from 1 mg/day in Spain to 530 mg/day in the UK, where it contributes to almost 50% of total flavonoids .
7. Flavanones: intake and food sources
Flavanones are normally the second main flavonoid class (8–10%) . The variation in flavanone intakes is relatively small between most of the countries (30–40 mg/day) (Table 2). The highest intake is in Greece and Spain (∼60 mg/day as aglycones) [27, 55], and the lowest intake is in China (∼5 mg/day) [24, 38]. Flavanone intake patterns perfectly match the consumption of their main food sources: citrus fruit and citrus juices .
8. Flavonols: intake and food sources
Flavonols are usually the third principal flavonoid class (7–9%) . The mean intake of flavonols generally varies between 20 and 40 mg/day (Table 2). In some countries (South Korea, China, and Poland) [25, 32, 62], a higher intake of flavonols is reported (>60 mg/day as aglycones), but some of these values are probably overestimated. Flavonols are widely distributed in fruit and vegetables, particularly apples, onions, some leafy vegetables, and red wine .
9. Anthocyanidins: intake and food sources
Anthocyanidins are present in all red‐blue‐purple fruits and vegetables (e.g., berries, apples, pears, grapes, plums, and aubergine) along with red wine. They are responsible for the color of these foods. Depending on the region, anthocyanidins fluctuate from the second to the fourth most consumed flavonoid class (7–10%) . The mean intake of anthocyanidins range from 20 to 40 mg/day in most of the countries (Table 2). Countries with a high consumption of berries, such as Finland, are the top consumers of anthocyanidins (∼50 mg/day) , while Brazil and the US have a particularly low intake (<15 mg/day) [20, 34, 35, 37, 60].
10. Flavones: intake and food sources
Flavones are the fifth most common flavonoid class, accounting for 1–2% of total flavonoids . Their intake varies between 1 and 5 mg/day using the USDA database  and 5–20 mg/day as aglycones using the Phenol‐Explorer  (Table 2). Flavones are widely distributed in plant‐based foods, fruits, vegetables, juices, wine, etc. It is important to highlight that the richest sources of flavones are herbal teas, and this food item is rarely recorded in dietary questionnaires, and therefore, an underestimation of flavone intake is quite probable.
11. Isoflavones: intake and food sources
Isoflavones are the flavonoid class with the lowest intake among the typical flavonoid classes (flavanols, flavanones, flavonols, anthocyanidins, flavones, and isoflavones). The intake of flavonoids is normally <2 mg/day in Westernized countries, as they mainly occur in soy products . However, in the UK health conscious group of the EPIC study, including vegans and vegetarians, the mean intake rises to 18 mg/day . In Asian countries, where the consumption of soy is very common, the mean intake of isoflavones ranges from 30 to 70 mg/day, contributing to almost 20% of total flavonoids.
12. Other minor flavonoids: intake and food sources
Chalcones, dihydrochalcones, and dihydroflavonols are three minor subclasses of flavonoids. Food composition data on these classes are limited and only available in Phenol‐Explorer , but not in the USDA database . Chalcones are found in beer and broad beans, and their mean intake is very low (<0.01 mg/day expressed as glycosides) (Table 2). Dihydrochalcones are present in apples and derived products (e.g., cider and apple juice). The mean intake is between 1.5 and 3 mg/day expressed as glycosides [17, 26], except in Poland where the intake is double (Table 2) [32, 33]. The principal food source of dihydroflavonols is wine, and therefore, the countries with a greater intake of wine, such as France and MED countries, have the highest consumption (∼5 mg/day expressed as glycosides) (Table 2) [17, 26]. Countries with a low wine intake, such as Poland, have a negligible intake (<0.1 mg/day expressed as glycosides) .
Overall, the mean intake of total flavonoids worldwide is around 400 mg/day, ranging from 150 mg/day in Latin‐American countries to 600 mg/day in Australia and the UK, without considering thearubigins. Tea (rich in flavan‐3‐ol monomers and thearubigins) is the most important food source of total flavonoids in countries with a tea culture, while fruits (rich in proanthocyanidins) are the main food source in the remaining countries. Flavanols is the main contributor to total flavonoids (75%), followed by far by flavanones, flavones, and anthocyanidins (7–10% each) and flavones and isoflavones (1–2% each). Chalcones, dihydrochalcones, and dihydroflavonols are also minor flavonoid subclasses. It is important to highlight that the intake of isoflavones is extremely high in East Asian countries (30–70 mg/day) compared to Western countries (<2 mg/day), since isoflavones mostly occur in soy products. Older women with healthy lifestyle and dietary habits and higher educational levels and incomes are the sector with the highest intake of total flavonoids.
Finally, more composition data are needed, particularly for tropical foods, to improve the estimation of total flavonoids, especially in some specific regions. Further studies are warranted to increase the descriptive analyses in several areas where little data are available to date, such as Latin America, Africa, and the Middle East. An accurate estimation of flavonoid intake is the first step before estimating their potential protective effects against chronic diseases and is an essential step for developing future dietary guidelines on flavonoids.
This project has been supported by the Instituto de Salud Carlos III project CP15/00100 and cofunded by FEDER funds/ European Regional Development Fund (ERDF)‐ a way to build Europe.
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