Flashcards in Non-standard decoding and ribosomal gymnastics Deck (16):
Give an example of a plant RNA virus
Tobacco mosaic virus (TMV)
What does TMV require?
An RNA-dependent RNA polymerase ('replicase')
Is TMV a positive or negative strand virus?
(+) strand virus, i.e. RNA acts as mRNA
What does the TMV encode?
A coat plus replicase etc.
What is the size of the TMV replicase protein?
The TMV replicase gene undergoes..
Programmed stop codon readthrough
The leaky UAG at the end of the replicase 'gene' product =
183 KDa = essential protein
What does a 'slippery sequence' do?
Causes ribosomes to slip backwards minus 1 frameshift
What efficiency does a -1 frameshift have?
Are retroviruses RNA or DNA viruses?
Is RNA translated by retroviruses?
Viral ssRNA is converted to what in retroviruses?
What enzyme do retroviruses need to convert ssRNA to DNA-RNA to dsDNA?
Reverse transcriptase (RT)
The dsDNA product produced by the reverse transcriptase of a retrovirus is integrated into..
The genome and expressed normally via transcription and translation
What are Gag and Pol products?