Antibodies protect nerve-muscle connections in a mouse model of Lou Gehrig’s disease
Now a new study led by NYU School of Medicine researchers identifies a novel treatment strategy that preserved neuromuscular synapses in a mouse model of aggressive ALS. Published February 20 in the journal eLife, the study found that the loss of such synapses was reduced in ALS mice injected with antibodies that stimulate a molecule in muscle which keeps nerves attached to the muscle.
By boosting the action of a protein called MuSK, the new approach slowed the loss of neuromuscular synapses, temporarily extended survival of motor neurons, and extended the lifespan of ALS mice.
“Our findings reveal a new therapeutic strategy for ALS that protects a pathway essential for keeping nerves and muscles connected,” says Steven Burden, PhD, a professor in the Skirball Institute of Biomolecular Medicine, and in the Department of Neuroscience and Physiology, at NYU School of Medicine.
“There are few treatments for ALS, and the two FDA-approved drugs extend survival for only a few months in a subset of patients,” says Burden. “We believe that our approach, mostly likely in combination with other drugs, may extend quality of life during the early phases of this disease.”
The results are based on the ability of neurons to pass messages to one another or to other targets, including skeletal muscle, at specialized junctions. Motor neurons connect to skeletal muscle at neuromuscular synapses, and the dismantling of these synapses is an early sign of disease in ALS. Although this dismantling is known to be a primary cause of ALS paralysis, most experimental treatments have sought to instead stop the death of motor neurons, which happens later in the disease course, researchers say.
The new approach centers on MuSK, a receptor tyrosine kinase on the muscle cell surface. Once stimulated by a signal provided by the nerve, MuSK provides instructions for building the neuromuscular synapse, including the attachment of motor nerve endings to muscle.
To test whether increasing attachment between nerve and muscle might keep synapses connected when they would otherwise be dismantled in ALS mice, the researchers used an antibody that stimulates MuSK to boost signaling from muscle to nerve. A single dose of a MuSK-stimulatory antibody, introduced into ALS mice after disease onset, increased the number of fully innervated neuromuscular synapses 2.6-fold.
Chronic dosing with the stimulatory antibody led to a sustained increase in the number of neuromuscular synapses for two months, and improved function of diaphragm muscle, which is critical for breathing. Untreated ALS mice survive for about five months, and the MuSK-stimulatory antibody prolonged their survival by about week.
“The therapeutic strategy described here targets a disease mechanism, namely the loss of neuromuscular synapses, which is common to familial and sporadic forms of ALS; and is based on a therapeutic antibody format with considerable clinical precedence,” says Burden. “Although the MuSK agonist antibody cannot override the many pathological pathways that occur in motor neurons and in non-neuronal cells, therapeutic interventions that preserve neuromuscular synapses have the potential to improve the quality of life for a majority of ALS patients.”
Along with Burden, the study was led by first author and Ph.D. student Sarah Cantor, along with postdoctoral fellows Wei Zhang and Leonor Remédio, in the Molecular Neurobiology Program, Helen L. and Martin S. Kimmel Center for Biology and Medicine, Skirball Institute of Biomolecular Medicine, NYU School of Medicine. Nicolas Delestrée, a postdoctoral fellow, and George Mentis, a faculty member, at the Center for Motor Neuron Biology and Disease at Columbia University, were key collaborators.
The MuSK agonist antibodies used in the study were provided by Genentech. The study was supported by grants from the National Institutes of Health (NIH) (R37 NS36193, RO1NS078375), the Robert Packard Center for ALS Research, and the ALS Association, along with funds from Above and Beyond, LLC, to Burden. Cantor was supported by an NIH training grant (T32 NS86750), and Mentis was also funded by the NIH (RO1 NS078375).
More information at https://nyulangone.org/