Animal Tissue Culture
The term "tissue culture" refers to the culture of whole of organs, tissue fragments as well as dispersed cells on a suitable nutrient medium.
It can be further subdivided into:
- Organ Culture
- Cell Culture
Primary culture: freshly isolated cultures; usually heterogenous and slow growing
Cell lines: subculturing of a primary culture gives cell lines. If the cell line dies after several subcultures- finite cell line. However if it continues to grow indefinately- continuous cell line.
1880 AD- Arnold showed that leucocytes can divide outside the body
1903 AD- Jolly studied the behavior of animal tissue explants immersed in serum, lymph, or ascites fluid.
1907 AD- Harrison cultured frog tadpole spinal chord in a lymph drop hanging from a cover slip of a cavity slide.
1913 AD- Carrel developed a complicated methodology for maintaining cultures free of contamination