562 , 2016-11-08 , 日本分析化学会 = Japan Society for Analytical Chemistry
email@example.com The quantity of microalgae biomass has been evaluated mainly based on the cell density and dry weight. However, these direct measurement methods requires much time and effort. Therefore, indirect methods have been developed for rapid and easier measurements. Major examples are methods that take chlorophyll to account, such as an absorbance measuring method and a fluorescence intensity method. Since there was a report that the chlorophyll content changes at various microalgae growth stages, the accuracy of the measurement through various growth stages must be confirmed. Therefore, in this study, we examined the compatibility and accuracy of three indirect measurement methods: absorbance measuring method, fluorescence intensity method, and Coulter Counter method at each growth stage of microalgae. As a results, each indirect methods showed a high correction coefficient to both the cell density and the dry weight; no influence was observed regarding the growth stage and the measurement accuracy. However, each indirect method had their own characteristics. By considering the microalgae characteristic and the growth environment, a more accurate measurement can be made.