The Enzyme-linked immunospot (ELISPOT) assay was developed by Czerkinsky in 1983. The ELISPOT assay is based on, and was developed from a modified version of the ELISA immunoassay. It is a sensitive method for monitoring immune responses in humans and animals and a superior tool for accurate quantification of cytokine secreting T cells or antibody secreting B cells.
Nowadays the ELISPOT assay is widely used in different fields of biomedical research including:
Follow these links to find out more about the application of the ELISPOT assay.