Vertical transmission is also mode of transmission of virus diseases. In pregnancy they may cause preterm delivery, abortion ,ectopic pregnancies, hepatitis ,cirrhosis and hepatocellular carcinoma. Janani Sureksha yojana is a scheme in which all pregnant women are benefited if they deliver in government medical facility .Antenatal screening for HBV,HCV and HIV should be done to diagnose viral infections . Present study was conducted in central laboratory of Rural Institute of Medical Science and Research Safai, Etawah (U.P) on pregnant women who attended the antenatal care under Janani Sureksha Yojna from 1January to31December 2014. The aim of study was to detect the frequency of HbsAg, HCV antibody,HIV antibody and their correlation with associated risk factors Out of 7867 women(2.07%)found positive for HbsAg, (0.43%),(0.13%)for HCV antibodies and HIV antibodies. Common age group was 21-30 years (68.48%). Among the clinical symptoms dominant symptom was fever(33.52%), .The associated risk factors were Obstetric and Gynaecology surgeries (46.15%) was prominent and found more among HbsAg sero positive females. The frequency of HbsAg (2.07%) was more. Fever, body ache were common clinical features. Obstetretic and Gynecology surgery, blood transfusion, were major associated risk factors. So, screening for Hbs Ag, HCV antibody, HIV antibody should be mandatory for pregnant women during antenatal care specially in rural areas to reduce the mortality and morbidity of pregnant women and their new ones.