Salk Institute researchers have developed a computational algorithm that integrates two different data types to make locating key regions within the genome more precise and accurate than other tools. The new method, detailed recently in PNAS through an article entitled “Improved regulatory element prediction based on tissue-specific local epigenomic signatures,” could help researchers conduct vastly more targeted searches for disease-causing genetic variants in the human genome, such as ones that promote cancer or cause metabolic disorders.
"Most of the variation between individuals is in noncoding regions of the genome," explained senior study investigator Joseph Ecker, Ph.D., a Howard Hughes Medical Institute investigator and director of Salk's Genomic Analysis Laboratory. "These regions don't code for proteins, but they still contain genetic variants that cause disease. We just haven't had very effective tools to locate these areas in a variety of tissues and cell types—until now."
Roughly only about two percent of our DNA is made up of genes, and for many years, the other 98 percent was thought to be extraneous "junk." But, as science has developed ever more sophisticated tools to probe the genome, it has become clear that much of that so-called junk has vital regulatory roles. For example, sections of DNA called "enhancers" dictate where and when the gene information turns on or off.
Increasingly, mutations or disruption in enhancers have been tied to major causes of human disease, but enhancers have been difficult to locate within the genome. Clues about them can be found in certain types of experimental data, such as binding proteins that regulate gene activity, chemical modifications of histones that DNA wraps around, or epigenetic regulation of DNA through DNA methylation.
Typically, computational methods for finding enhancers have relied on histone modification data. However, the Salk’s new system, called REPTILE (for "regulatory element prediction based on tissue-specific local epigenomic signatures"), combines histone modification and methylation data to predict which regions of the genome contain enhancers. In the team's experiments, REPTILE proved more accurate at finding enhancers than algorithms that rely on histone modification alone.
"The novelty of this method is that it uses DNA methylation to really narrow down the candidate regulatory sequences suggested by histone modification data," noted lead study investigator Yupeng He, a graduate student at the Salk Institute. "We were then able to test REPTILE'S predictions in the lab and validate them with experimental data, which gave us a high degree of confidence in the algorithm's ability to find enhancers."
The REPTILE algorithm operates in two general steps: training, and prediction. For training, the Salk team taught REPTILE to recognize mammalian enhancers by feeding into the algorithm both the locations of known enhancers as well as genomic areas other than enhancers in the DNA. In the prediction step, the algorithm ran on nine mouse and five human cell lines and tissues whose enhancer regions were unknown, and pinpointed the locations of potential enhancers. Finally, the team used data from laboratory experiments to test whether the predictions made by REPTILE in the prediction step corresponded to real regulatory regions.
Since enhancers increase the activity of target genes, researchers can test the activity of DNA sequences by connecting them to a reporter gene and watching to see whether the supposed target gene ramps up. Using molecular tools, the team engineered mouse embryos so that enhancer activation would trigger the expression of linked reporters, which can be monitored by staining. So, if REPTILE predicted that a specific enhancer was linked to mouse forebrain development, the team was able to look for a staining pattern in the embryo's forebrain region. If they saw it, REPTILE's prediction was considered valid.
Moreover, the Salk team tested REPTILE's predictions against four other commonly used enhancer-finding algorithms. Overall, REPTILE outperformed each one, finding enhancer regions with greater accuracy (getting closer to them along the DNA strand) and fewer errors (misidentifications). In particular, REPTILE was more successful than the other systems at the invaluable task of finding enhancers in different tissue types than those it was trained on.
"The number of genetic variants in the genome is enormous," Dr. Ecker remarked. "So, in terms of finding ones that cause disease, you really want to shine a spotlight on the regions you think are most important and identifying enhancers is a critical step in the process."