September 10, 2017 Srijana Khanal Lab Diagnosis of Viral Disease, Virology 3. Animal identification using a means of marking is a process done to identify and track specific animals.It is done for a variety of reasons including verification of ownership, biosecurity control, and tracking for research or agricultural purposes. Identification Methods for Mice Guideline 01.3. Purpose - Researchers must consider all sources of DNA to perform genotype analysis, including alternatives to invasive procedures such as tail biopsy. Always test before animal use. As with any procedure, the specific method of tissue collection must be detailed in the approved IACUC protocol. College of health sciences-HMU Lab 1 2. It is often said that molecular methods is the future direction of viral diagnosis. Read more about flow cytometry. Ear Punching The identification method of choice for the NHGRI Animal Care and Use Committee is ear punching. INSTITUTE FOR LAbORATORy ANIMAL RESEARCH PUbLICATIONS. The survey results indicate that mouse is the overwhelmingly preferred laboratory animal; the most widely used mouse and rat strains are C57BL/6 mice, BALB/c mice, Sprague-Dawley rats and Wistar rats. Given the continued reliance on microscopy and culture, the diagnostic value of these methods must be improved by procedural changes and adequate training of laboratory personnel. Laboratory diagnosis of bacteria Prepared by Samira Fattah Assis. Guidelines for Genotyping Laboratory Mice and Rats. ☆ Requires approval from the USDA (Animal Care Resource Guide policy #13). In conclusion, the sequence analysis of 16S rDNA proved to be a useful diagnostic tool, with higher performance characteristics than the classical phenotypic methods, for identification of laboratory animal bacteria. Salmonella serotyping is a subtyping method of identification based on the identification of distinct cell wall, flagellar, and capsular antigens with known antiserum, as will be discussed in Lab 17. The techniques described will facilitate the most efficient use of research animals and provide guidelines for their utmost comfort and welfare. Rodent Identification Methods There are several acceptable methods to permanently identify laboratory rodents. The pure growth from the secondary culture can then be used for identification of the organism using biochemical tests, etc. Methods of bacterial identification • Microscopic examination. ... cultured cells derived from animals and humans are used for viral growth • Immunological methods. Definitions. Methods of Animal Experimentation, Volume I, provides information on the most common methods for using animals as tools in the search for new biological knowledge. Bacterial Identification methods. So either we identify them using a microscope or by enhancing their population to a huge bulk or by biochemical methods. • Animal inoculation. Laboratory diagnosis of bacteria 1. When activated by a compatible reader, the passive, battery-free transponder provides a unique, non-repeating, alphanumeric code that allows accurate identification of individual animals in a group. Laboratory confirmation may be based on direct or indirect methods. Flow cytometry is a laboratory method used to detect, identify, and count specific cells from blood, bone marrow, body fluids such as cerebrospinal fluid (CSF), or tumors. Animal Cell Culture: Introduction, Types, Methods and Applications Animal Cell Culture: Introduction, Types, Methods and Applications. Easy Integration With Your Database. Although embroyonated eggs and laboratory animals are very useful for the isolation of certain viruses, cell cultures are the sole system for virus isolation in most laboratories. Recognition and Alleviation of Pain in Laboratory Animals (2009) Scientific and Humane Issues in the Use of Random Source Dogs and Cats for Research (2009) Recognition and Alleviation of Distress in Laboratory Animals (2008) Toxicity Testing in the 21st Century: A Vision and a Strategy (2007) In a research community with high technological standards and demands, ELAMS provides positive animal identification and simplifies daily lab procedures. There are several permanent and temporary methods that scientists use to identify individual lab animals. UID Laboratory Animal Microchips offer a reliable, convenient and permanent method for positive identification of laboratory animals. Here, we are going to discuss the two most commonly used methods: ear tagging and ear punching. • They work well for a short time but are easily removed by some animals. I. • Bands are also used as permanent identification • Biochemical reactions. The Federation of European Laboratory Animal Science Associations (FELASA) published a report meant to assist investigators in choosing the most appropriate identification method for studies. Distinguishing between animals ensures that the correct subject is used for the intended experimental procedure. EAR PUNCH: This is a commonly used procedure which employs a special metal The collection, handling, identification, and reporting of ectoparasitic arthropods in clinical and reference diagnostic laboratories are discussed in this review. Chapter 6 Diagnostic microbiology and laboratory methods. FELASA advocates accreditation of laboratory animal diagnostic laboratories according to DIN EN ISO/IEC 17025 (formerly ISO Guide 25), in which special emphasis is placed on competency of the staff, validation of test methods, and participation in interlaboratory testing [48, 75]. Traditional methods have relied on the identification of morphological traits, but this does not lead to accurate identification of those species used in many types of processed food. Laboratory Animal Science. Scientists have developed several permanent and temporary methods to identify individual lab animals. Microscopy Microscopic methods, such as wet mounts, Gram stains, and conventional histopathology, provide clues that suggest the presence of Aspergillus spp. ... commonly occur in cell culture creating a problem to identify and eliminate. A description of the identification method used must be included in answer Section A #15 of your approved University Committee on Animal Resources (UCAR) protocol. The following means of animal identification may be requested in the ASP. The industry standard for lab animal identification. Included are data on ticks, mites, lice, fleas, myiasis-causing flies, and bed bugs. ¤ Specific tag dimensions and/or numbering/lettering requirements are provided by the USDA. For the first time this method allowed us to document the association of certain bacterial species with the laboratory mouse. One of the most common applications is in the diagnosis of leukemia and lymphoma. Labome has surveyed formal publications citing animal models to provide an overview of animal models in publications (Table 1). Permanent Identification Methods Ear Punching. Ergonomic form factors, easy-reading display panels, and extensive connectivity options facilitating Lab Animal Identification. The ability to identify individual research animals is an essential aspect of scientific recordkeeping and data collection. All identification methods for mice must be approved in the Animal Study Proposal ASP. Ear punching is commonly used for identification of rodents and involves using a special punch to either produce a small (0.5 to 2 mm) notch near the edge or to punch a hole in the middle of the ear. 1. This is especially true for genetically altered (GA) animals where individual identification is strictly necessary to link samples, research design and genotype. DOI: 10.1177/002367712473290 Rodent identiﬁcation and animal welfare The aim of this Federation of European Laboratory Animal Science Associations (FELASA) Working Group was to identify best practices for rodent identiﬁcation in different situations and assess their implications for animal welfare. • Cultural appearance. • Serological identification. The simplest and most cost effective way to automate your vivarium and data collection is to use RapID Tags – the state-of-the-art lab animal identification choice of leading companies and institutions worldwide. Eurofins offers a comprehensive spectrum of methods for the identification of animal species. Laboratory Animals 2013; 47: 2–11. Whether you are using Excel or a robust vivarium management software … Hence in the identification of bacteria, we use microscopical, medical, biochemical, & serological methods. Lec. Laboratory Animal Resources . * Requires anesthesia or sedation to perform this method of identification. The public health importance of these organisms is briefly discussed. The AIMS™ ATS-3L Large Lab Animal Tattoo System is a compact tattoo kit specifically developed for the tattoo identification of large laboratory animals (hamsters, guinea pigs, ferrets, rabbits, NHP, Swine, [anything larger than a mouse/rat]).Features AIMS™ ultra light weight tattoo machine for quick, easy skin tattooing for both the tattooist and animal. THERE are currently a number of different methods for identifying rabbits within a variety of contexts. in tissue. Identification of the agent: Clinical lesions (epididymitis and orchi-epididymitis) in rams may be indicative of the existence of infection, but laboratory examinations are required to confirm the disease. identify animals as a temporary method—the adhesive paper bands are placed around the animal’s neck with the name of the animal and the and owner on them. Direct diagnosis is Bacteria as mentioned before or so tiny that one cannot watch without a microscope. Moreover, psychologists trained in appropriate research methods and experienced in the care of laboratory animals must supervise all procedures involving these animals and are "responsible for ensuring appropriate consideration of their comfort, health and humane treatment," says the code. RapID Tag’s unique automated design provides instant identification with 100% accuracy. Euthanasia - the act of inducing humane death in an animal by a method that induces rapid loss of consciousness and death with a minimum of pain, discomfort or distress. The development of methods for cultivating animal cells has been essential to the progress of animal virology. Methods based on the detection of viral genome are also commonly known as molecular methods.