Objective: To investigate antibiotic prescribing pattern in bacterial, viral and allergic conjunctivitis in a tertiary care hospital. Materials and Methods: Prospective-open labelled, non-interventional observational study, total 100 patients were recruited for the study, all antibiotics prescribed were recorded including dosage form, frequency and duration, disease condition whether bacterial, viral and allergic conjunctivitis was taken into account. These forms were used to analyse average utilization of antibiotics in various forms of conjunctivitis, descriptive statistics performed. Data was entered and analysed with Microsoft Excel 2013. Values were expressed as actual numbers and percentage Results: Total numbers of drugs prescribed are 200 out of 100 prescriptions. Most commonly prescribed drugs are topical antibiotics followed by antihistamines and analgesics and then steroids, antivirals are prescribed less, among antibiotics, and fluoroquinolones were prescribed most common followed by aminoglycosides and broad spectrum antibiotics such as chloramphenicol and tetracyclines Conclusion: Besides rational antibiotic use, the prevention of bacterial infections and their spread are essential in the containment of antibiotic resistance. Ophthalmic antibiotics should be prescribed judiciously in acute infective conjunctivitis even for suspected bacterial cases; since it is found to resolve spontaneously without any serious complications.