Innovative genetic approach may give crops resistance to charcoal rot disease
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Forster, Heather. 2019. Innovative genetic approach may give crops resistance to charcoal rot disease -- In Proceedings: 15th Annual Symposium on Graduate Research and Scholarly Projects. Wichita, KS: Wichita State University
Macrophomina phaseolina, causative agent of the plant disease charcoal rot, impacts over 500 plant species, causing devastating crop failures worldwide. In Kansas, it is often the biggest cause of soybean crop loss, and disease epidemics are increasingly frequent. Charcoal rot attacks primarily through fungus-infested soil, leading to rot of the stem, roots, fruits, seeds, and leaves. Traditional pathogen control means, such as natural resistance, crop rotation, and fungicides, have been ineffective or problematic. This study aims to evaluate the effectiveness of host-delivered RNA interference (HD-RNAi) to manage charcoal rot. HD-RNAi exploits the natural process of RNA interference to target essential genes for M. phaseolina. In this process, small interfering RNAs (siRNAs) are designed and engineered into plant genomes. Upon infection, siRNAs expressed in plant cells can enter invading fungus and prevent expression of genes necessary for successful infection. HD-RNAi has been successful against some nematodes, insects, and other fungi. In this preliminary work, we have manufactured siRNAs to interfere with genes required for the synthesis of M. phaseolina cell wall compounds, CHS6 and GLS2. We hypothesize that without these compounds, the fungus will be unable to grow and infect plants. To test siRNA effectiveness, we incubated the fungus with siRNAs and measured its growth. To date, we have observed apparent suppression of growth in fungus treated with siRNAs against GLS2 at early timepoints. We will follow this work with microscopic fungal examinations and quantitative confirmation of gene suppression. Our work gives insights into RNA interference in M. phaseolina and provides a framework for future siRNA testing.
2nd place award winner in the poster presentations at the 15th Annual Symposium on Graduate Research and Scholarly Projects (GRASP) held at the Rhatigan Student Center, Wichita State University, April 26, 2019.
Research completed in the Department of Biological Sciences, Fairmount College of Liberal Arts and Sciences