Verticillium dahliae, causal agent of Verticillium wilt of potato, persists in soil as microsclerotia and can be found in infected tubers used for seed. The effects of naturally infected tubers and soilborne inoculum on Verticillium wilt symptoms were compared in the greenhouse. Infected and noninfected tubers were grown in infested and noninfested potting soil. Chlorosis and necrosis were measured and converted to area under senescence progress curves (AUSPC). Aboveground stems and progeny tubers were assayed for V. dahliae. Plants from infested soils exhibited significantly greater AUSPC than plants from noninfested soil. Plants grown from infected and noninfected tubers had similar AUSPC and interactions between infected tubers and infested soil were not observed. The pathogen was isolated from the vascular system of 94% of plants grown in infested soils and 8% of plants grown from infected tubers in noninfested soil. Plants grown in infested soil contained microsclerotia on 46% of stems while plants grown from infected tubers in noninfested soils exhibited microsclerotia on <1% of stems. Infected progeny tubers were only recovered from plants grown in infested soil. Seed tuber infection did not contribute to premature senescence or potential inoculum production, indicating that management efforts should focus on reducing soilborne inoculum.