Figure 5: B cells and immunoglobulins isotype during the development of enterocolitis. Cells from spleen (SP), bone marrow (BM), Peritoneum (PT), Peyer patches (PP), or lamina propria (LP) from small or large intestine were obtained from 16-week-old IL-10−/− mice. Frequencies of CD19+ B cells (a) or CD19+CD5+ (b) gated on lymphocytes or CD19+ population, respectively, were assessed by flow cytometry. Bars represent the mean ± SEM of 4 mice per group. Asterisks represent difference between groups (). Sera from 4-, 6-, 10-, or 16-week-old IL-10−/− mice were collected and total IgM (c), IgG (d), IgA (e), and IgE (f) were measured by ELISA. A.U.: arbitrary units. Bars represent the mean ± SEM of 5 mice per group. Significant differences (*, ** or ***) are indicated by asterisks.