The burden of cervical cancer is increasing in Vietnam in the recent years, infection with high risk HPVbeing the cause. This study aimed to examine the prevalence of HPV and the distribution of HPV specific typesamong the general population in 5 big cities in Vietnam. Totals of 1500 women in round 1 and 3000 in round 2were interviewed and underwent gynecological examination. HPV infection status, and HPV genotyping testwere perfoirmed for all participants. Results indicated that the prevalence of HPV infection in 5 cities rangedfrom 6.1% to 10.2% with Can Tho having highest prevalence. The most common HPV types in all 5 cities wereHPV 16, 18 and 58. Most of the positive cases were infected with high risk HPV, especially in Hanoi and Can Thowhere more than 90% positive cases were high risk HPV. Furthermore, in Can Tho more than 60% of womenwere infected with multiple HPV types. The information from this study can be used to provide updated datafor planning preventive activities for cervical cancer in the studied cities.