Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-F.
Adenovirus Infections, Human
Adenovirus E1A Proteins
Adenovirus Early Proteins
Proteins encoded by adenoviruses that are synthesized prior to, and in the absence of, viral DNA replication. The proteins are involved in both positive and negative regulation of expression in viral and cellular genes, and also affect the stability of viral mRNA. Some are also involved in oncogenic transformation.
Adenovirus E1B Proteins
Adenovirus E3 Proteins
Adenovirus E4 Proteins
Adenovirus E1 Proteins
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Adenovirus E2 Proteins
Fowl adenovirus A
Gene Transfer Techniques
The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.
Tumor-selective, replication competent VIRUSES that have antineoplastic effects. This is achieved by producing cytotoxicity-enhancing proteins and/or eliciting an antitumor immune response. They are genetically engineered so that they can replicate in CANCER cells but not in normal cells, and are used in ONCOLYTIC VIROTHERAPY.
Oncogene Proteins, Viral
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Cell Transformation, Viral
Cytopathogenic Effect, Viral
Visible morphologic changes in cells infected with viruses. It includes shutdown of cellular RNA and protein synthesis, cell fusion, release of lysosomal enzymes, changes in cell membrane permeability, diffuse changes in intracellular structures, presence of viral inclusion bodies, and chromosomal aberrations. It excludes malignant transformation, which is CELL TRANSFORMATION, VIRAL. Viral cytopathogenic effects provide a valuable method for identifying and classifying the infecting viruses.
Viruses which lack a complete genome so that they cannot completely replicate or cannot form a protein coat. Some are host-dependent defectives, meaning they can replicate only in cell systems which provide the particular genetic function which they lack. Others, called SATELLITE VIRUSES, are able to replicate only when their genetic defect is complemented by a helper virus.
Promoter Regions, Genetic
Gene Expression Regulation, Viral
Tumor Cells, Cultured
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Simian virus 40
Amino Acid Sequence
Recombinant Fusion Proteins
Respiratory Tract Infections
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Antigens, Viral, Tumor
Inclusion Bodies, Viral
An area showing altered staining behavior in the nucleus or cytoplasm of a virus-infected cell. Some inclusion bodies represent "virus factories" in which viral nucleic acid or protein is being synthesized; others are merely artifacts of fixation and staining. One example, Negri bodies, are found in the cytoplasm or processes of nerve cells in animals that have died from rabies.
DNA Restriction Enzymes
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Nucleic Acid Hybridization
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Cell Line, Transformed
Gene Expression Regulation
This line KB is now known to be a subline of the ubiquitous KERATIN-forming tumor cell line HeLa. It was originally thought to be derived from an epidermal carcinoma of the mouth, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via contamination by HELA CELLS. The cells are positive for keratin by immunoperoxidase staining. KB cells have been reported to contain human papillomavirus18 (HPV-18) sequences.
Xenograft Model Antitumor Assays
Green Fluorescent Proteins
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).
Cell Transformation, Neoplastic
NFI Transcription Factors
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Defective viruses which can multiply only by association with a helper virus which complements the defective gene. Satellite viruses may be associated with certain plant viruses, animal viruses, or bacteriophages. They differ from satellite RNA; (RNA, SATELLITE) in that satellite viruses encode their own coat protein.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Tumor Suppressor Protein p53
Y-Box-Binding Protein 1
An alpha integrin with a molecular weight of 160-kDa that is found in a variety of cell types. It undergoes posttranslational cleavage into a heavy and a light chain that are connected by disulfide bonds. Integrin alphaV can combine with several different beta subunits to form heterodimers that generally bind to RGD sequence-containing extracellular matrix proteins.
Viral Plaque Assay
Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Complement Fixation Tests
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
Product of the retinoblastoma tumor suppressor gene. It is a nuclear phosphoprotein hypothesized to normally act as an inhibitor of cell proliferation. Rb protein is absent in retinoblastoma cell lines. It also has been shown to form complexes with the adenovirus E1A protein, the SV40 T antigen, and the human papilloma virus E7 protein.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Eye Infections, Viral
Infections of the eye caused by minute intracellular agents. These infections may lead to severe inflammation in various parts of the eye - conjunctiva, iris, eyelids, etc. Several viruses have been identified as the causative agents. Among these are Herpesvirus, Adenovirus, Poxvirus, and Myxovirus.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
An enzyme that catalyzes the acetylation of chloramphenicol to yield chloramphenicol 3-acetate. Since chloramphenicol 3-acetate does not bind to bacterial ribosomes and is not an inhibitor of peptidyltransferase, the enzyme is responsible for the naturally occurring chloramphenicol resistance in bacteria. The enzyme, for which variants are known, is found in both gram-negative and gram-positive bacteria. EC 188.8.131.52.
Centrifugation, Density Gradient
Reverse Transcriptase Polymerase Chain Reaction
E1A-Associated p300 Protein
Open Reading Frames
Antigens, Polyomavirus Transforming
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Disease Models, Animal
A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)
A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, infecting the salivary glands, liver, spleen, lungs, eyes, and other organs, in which they produce characteristically enlarged cells with intranuclear inclusions. Infection with Cytomegalovirus is also seen as an opportunistic infection in AIDS.
Enterovirus B, Human
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
The production of PEPTIDES or PROTEINS by the constituents of a living organism. The biosynthesis of proteins on RIBOSOMES following an RNA template is termed translation (TRANSLATION, GENETIC). There are other, non-ribosomal peptide biosynthesis (PEPTIDE BIOSYNTHESIS, NUCLEIC ACID-INDEPENDENT) mechanisms carried out by PEPTIDE SYNTHASES and PEPTIDYLTRANSFERASES. Further modifications of peptide chains yield functional peptide and protein molecules.
Viral Core Proteins
Sequence Analysis, DNA
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
Enhancer Elements, Genetic
E2F Transcription Factors
A family of basic helix-loop-helix transcription factors that control expression of a variety of GENES involved in CELL CYCLE regulation. E2F transcription factors typically form heterodimeric complexes with TRANSCRIPTION FACTOR DP1 or transcription factor DP2, and they have N-terminal DNA binding and dimerization domains. E2F transcription factors can act as mediators of transcriptional repression or transcriptional activation.
Late transcription and simultaneous replication of simian adenovirus 7 DNA as revealed by spreading lytically infected cell cultures. (1/60)Miller's technique of spreading DNA was applied to monkey cells productively infected with simian adenovirus 7. This permitted the visualization of cellular DNA transcription, both nucleolar and non-nucleolar, and of late transcription and replication of virus. Virus double-stranded DNA, thin fibres with very few nucleosome-like particles, were observed carrying either transcription or replication complexes. In addition, both RNP transcripts and replication forks were found on some virus duplex DNA. Virus single-stranded DNA replicative intermediates were identified on the basis of their increased thickness and contrast which results from the presence of a DNA binding protein. (+info)
Biological activity of the intact and cleaved DNA of the simian adenovirus 7. (2/60)Only the deproteinized DNA preparations of the simian adenovirus of the type 7 (SA 7) exhibited transforming and tumorigenic activity. The complex of the SA7 DNA with terminal protein (TP) did not exhibit either transforming or tumorigenic activity in cell cultures. In contrast to the transforming potential the infectious titers of the DNA - TP complex for the monkey kidney cells were 30-50 times higher than those of pure DNA. Cleavage of the SA7 DNA by specific endonucleases enhanced the tumorigenic potential of pure DNA, suppressed its infectivity and did not affect the lack of transformation capacity of the DNA - TP complex. The onc-gene was localized in the left terminal fragment with the minimal size 4,3x10(6)D in the case of R.Sal I. The tumorigenic activity was found to decrease with an increase in the size of the DNA fragment containing the onc-gene. (+info)
Chimpanzee adenovirus CV-68 adapted as a gene delivery vector interacts with the coxsackievirus and adenovirus receptor. (3/60)A replication-defective form of chimpanzee adenovirus type 68 (C68) has been developed to circumvent problems posed by widespread preexisting immunity to common human adenovirus vectors. To investigate the determinants of C68 tropism, its interaction with the coxsackievirus and adenovirus receptor (CAR) was studied. Although CHO cells were resistant to transduction by C68 as well as by adenovirus type 5 (Ad5), CHO cells expressing either human or murine CAR were transduced readily. C68 transduction, like Ad5 transduction, was blocked when cells were exposed to anti-CAR antibody or when virus was exposed to a soluble form of the CAR extracellular domain. These results indicate that gene delivery by C68 occurs by a CAR-dependent mechanism. (+info)
Novel, chimpanzee serotype 68-based adenoviral vaccine carrier for induction of antibodies to a transgene product. (4/60)An E1-deletion-containing adenoviral recombinant based on the chimpanzee serotype 68 (AdC68) was developed to express the rabies virus glycoprotein. Mice immunized with this construct (AdC68rab.gp) developed antibodies to rabies virus and remained resistant to challenge with an otherwise lethal dose of rabies virus. In naive mice immunized intranasally, the rabies virus-specific antibody responses elicited by AdC68rab.gp were comparable with regard to both titers and isotype profiles to those induced by an adenoviral recombinant based on human serotype 5 (Adhu5) expressing the same transgene product. In contrast, subcutaneous immunization with the AdC68rab.gp vaccine resulted in markedly lower antibody responses to the rabies virus glycoprotein than the corresponding Adhu5 vaccine. Antibodies from AdC68rab.gp-immunized mice were strongly biased towards the immunoglobulin G2a isotype. The antibody response to the rabies virus glycoprotein presented by Adhu5rab.gp was severely compromised in animals preexposed to the homologous adenovirus. In contrast, the rabies virus-specific antibody response to the AdC68rab.gp vaccine was at most marginally affected by preexisting immunity to common human adenovirus serotypes, such as 2, 4, 5, 7, and 12. This novel vaccine carrier thus offers a distinct advantage over adenoviral vaccines based on common human serotypes. (+info)
Comparative sequence analysis of the largest E1A proteins of human and simian adenoviruses. (5/60)The early region 1A (E1A) gene is the first gene expressed after infection with adenovirus and has been most extensively characterized in human adenovirus type 5 (hAd5). The E1A proteins interact with numerous cellular regulatory proteins, influencing a variety of transcriptional and cell cycle events. For this reason, these multifunctional proteins have been useful as tools for dissecting pathways regulating cell growth and gene expression. Despite the large number of studies using hAd5 E1A, relatively little is known about the function of the E1A proteins of other adenoviruses. In 1985, a comparison of E1A sequences from three human and one simian adenovirus identified three regions with higher overall levels of sequence conservation designated conserved regions (CR) 1, 2, and 3. As expected, these regions are critical for a variety of E1A functions. Since that time, the sequences of several other human and simian adenovirus E1A proteins have been determined. Using these, and two additional sequences that we determined, we report here a detailed comparison of the sequences of 15 E1A proteins representing each of the six hAd subgroups and several simian adenoviruses. These analyses refine the positioning of CR1, 2, and 3; define a fourth CR located near the carboxyl terminus of E1A; and suggest several new functions for E1A. (+info)
A simian replication-defective adenoviral recombinant vaccine to HIV-1 gag. (6/60)In animal models, E1-deleted human adenoviral recombinants of the serotype 5 (AdHu5) have shown high efficacy as vaccine carriers for different Ags including those of HIV-1. Humans are infected by common serotypes of human adenovirus such as AdHu5 early in life and a significant percentage has high levels of neutralizing Abs to these serotypes, which will very likely impair the efficacy of recombinant vaccines based on the homologous virus. To circumvent this problem, a novel replication-defective adenoviral vaccine carrier based on an E1-deleted recombinant of the chimpanzee adenovirus 68 (AdC68) was developed. An AdC68 construct expressing a codon-optimized, truncated form of gag of HIV-1 induces CD8(+) T cells to gag in mice which at the height of the immune response encompass nearly 20% of the entire splenic CD8(+) T cell population. The vaccine-induced immune response provides protection to challenge with a vaccinia gag recombinant virus. Induction of transgene-specific CD8(+) T cells and protection against viral challenge elicited by the AdC68 vaccines is not strongly inhibited in animals preimmune to AdHu5 virus. However, the response elicited by the AdHu5 vaccine is greatly attenuated in AdHu5 preimmune animals. (+info)
Complete nucleotide sequences and genome organization of four chimpanzee adenoviruses. (7/60)The complete nucleotide sequences for four adenoviruses-Simian Adenoviruses 21, 22, 23, and 24, originally isolated from chimpanzees, were determined. The genome organization of the chimpanzee adenoviruses was found to be similar to that of other adenoviruses. The viral gene products of the adenoviruses Simian Adenoviruses 22, 23, and 24 are very closely related to those of the (previously sequenced) chimpanzee adenovirus Simian Adenovirus 25. Simian Adenovirus 21 is most similar to human subgroup B adenoviruses HAdV-3, HAdV-7, and HAdV-35. Analysis of the capsid proteins hexon and fiber of the chimpanzee adenoviruses also supports the placement of Simian Adenovirus 21 in subgroup B and Simian Adenoviruses 22, 23, and 24 in subgroup E. (+info)
Analysis of the first complete genome sequence of an Old World monkey adenovirus reveals a lineage distinct from the six human adenovirus species. (8/60)Simian adenovirus 3 (SAdV-3) is one of several adenoviruses that were isolated decades ago from Old World monkeys. Determination of the complete DNA sequence of SAdV-3 permitted the first full genomic comparison of a monkey adenovirus with adenoviruses of humans (HAdVs) and chimpanzees, which are recognized formally as constituting six of the species (HAdV-A to HAdV-F) within the genus Mastadenovirus. The SAdV-3 genome is 34 246 bp in size and has a G+C content of 55.3 mol%. It contains all the genes that are characteristic of the genus Mastadenovirus and has a single VA-RNA gene and six genes in each of the E3 and E4 regions. The genetic organization is the same as that of HAdV-12, a member of the HAdV-A species. Phylogenetic analyses showed that although SAdV-3 is related marginally more closely to HAdV-A and HAdV-F than to other species, it represents a unique lineage that branched at an early stage of primate adenovirus divergence. The results imply that the genetic layout in SAdV-3 and HAdV-12 may also have characterized the common ancestor of all sequenced primate adenoviruses. (+info)
Adenoviruses May Pose Risk for Monkey-to-Human Leap - Healthcanal.com : Healthcanal.com
Last year, Chiu and colleagues also identified another new adenovirus, named simian adenovirus C, which sickened four of nine captive baboons and killed two of them at a primate facility in 1997. Several staff members at the facility also complained of upper respiratory symptoms at the time of the outbreak. Re-examining the samples many years later, Chiu and his colleagues found antibodies targeted to simian adenovirus C in the human samples.. Chiu concluded that staff members had been exposed to the new virus, and that the virus may have jumped from baboon to human, an idea also supported by follow-up experiments in which laboratory strains of simian adenovirus C efficiently infected both human and baboon cells.. Adenoviruses to date have not generally been linked to cross-species infections between monkeys and humans, Chiu said.. In light of these findings, however, he said the normal vigilance in tracking animal viruses that might also infect humans should extend beyond influenza and ...
A pan-genotype HCV T cell vaccine, in a simian adenovirus vector, to target T cell epitopes conserved across multiple HCV...
Protective Efficacy of a Novel Simian Adenovirus Vaccine Against Lethal MERS-CoV Challenge in a Transgenic Human DPP4 Mouse...
Welcome to CDC stacks | Chimpanzee Adenovirus Antibodies in Humans, Sub-Saharan Africa - 7924 | CDC Public Access | Emerging...
Human sera from the United States, Thailand, and sub-Saharan Africa and chimpanzee sera were tested for neutralizing antibodies to 3 chimpanzee adenoviruses. Antibodies were more common in humans residing in sub-Saharan Africa than in humans living in the United States or Thailand. This finding suggests cross-species transmission of chimpanzee adenoviruses ...
Safety and Immunogenicity of New Malaria Vaccine Candidate ChAd63 CS Administered Alone and With MVA CS - Full Text View -...
This is an open label phase I study, to assess the safety of a novel malaria vaccine, AdCh63 CS, simian adenovirus encoding Plasmodium falciparum liver stage antigen, Circumsporozoite protein. All volunteers recruited will be healthy adults. They will be primed with various doses of AdCh63 CS administered intramuscularly. Some of the volunteers will receive a booster vaccination with MVA CS administered via intramuscular route. Safety data will be collected for each vaccination regimen. Secondary aim of this study will be to assess the immune responses generated by vaccination ...
Vaccine-elicited human T cells recognizing conserved protein regions inhibit HIV-1 - NDM Research Building
Virus diversity and escape from immune responses are the biggest challenges to the development of an effective vaccine against HIV-1. We hypothesized that T-cell vaccines targeting the most conserved regions of the HIV-1 proteome, which are common to most variants and bear fitness costs when mutated, will generate effectors that efficiently recognize and kill virus-infected cells early enough after transmission to potentially impact on HIV-1 replication and will do so more efficiently than whole protein-based T-cell vaccines. Here, we describe the first-ever administration of conserved immunogen vaccines vectored using prime-boost regimens of DNA, simian adenovirus and modified vaccinia virus Ankara to uninfected UK volunteers. The vaccine induced high levels of effector T cells that recognized virus-infected autologous CD4 + cells and inhibited HIV-1 replication by up to 5.79 log 10. The virus inhibition was mediated by both Gag- and Pol- specific effector CD8 + T cells targeting epitopes that are
Difference between revisions of Chimpanzee Evolution - microbewiki
While chimpanzees can give insight into common illnesses, they can also be integral in the development of vaccines. Adenoviruses (Ads), a type of double-stranded DNA virus that broadly infects vertebrates, have historically been used as vaccine vectors. Although human serotypes of adenovirus (AdHus) have frequently been used, studies of chimpanzee adenoviruses (AdCs) are rising in prevalence due to a number of apparent benefits, including increased immunogenicity and a decrease in neutralizing cell responses in comparison to human adenoviral vectors.  Chimpanzee adenovirus vectors have been developed in response to a number of pervasive and otherwise common diseases; in response to the 2016 Ebola outbreak, for example, researchers developed AdCs that successfully activated memory T-cell responses, distributed equally between CD4 and CD8 T-cells.  In January 2017, the Coalition for Epidemic Preparedness Innovations (CEPI) was formed with the purpose of creating and stockpiling ...
Difference between revisions of Chimpanzee Evolution - microbewiki
While chimpanzees can give insight into common illnesses, they can also be integral in the development of vaccines. Adenoviruses (Ads), a type of double-stranded DNA virus that broadly infects vertebrates, have historically been used as vaccine vectors. Although human serotypes of adenovirus (AdHus) have frequently been used, studies of chimpanzee adenoviruses (AdCs) are rising in prevalence due to a number of apparent benefits, including increased immunogenicity and a decrease in neutralizing cell responses in comparison to human adenoviral vectors.  Chimpanzee adenovirus vectors have been developed in response to a number of pervasive and otherwise common diseases; in response to the 2016 Ebola outbreak, for example, researchers developed AdCs that successfully activated memory T-cell responses, distributed equally between CD4 and CD8 T-cells. In January 2017, the Coalition for Epidemic Preparedness Innovations (CEPI) was formed with the purpose of creating and stockpiling ...
Vaxzevria Vaccine (ex Covid-19 Vaccine AstraZeneca) | Italian Medicines Agency
Vaxzevria Vaccine is used to prevent coronavirus disease 2019 (COVID-19) in people from 18 years of age. It has been designed to prepare the immune system to identify and combat the coronavirus SARS-CoV-2 causing COVID-19.. The vaccine is made up of a chimpanzee adenovirus (ChAdOx1 - Chimpanzee Adenovirus Oxford 1) that is unable to replicate and that has been modified to contain the genetic information for producing the SARS-CoV-2 spike protein.. The viral vector technology used for this vaccine has already been successfully tested and has been employed in the prevention of other diseases.. ...
COVID-19 | AstraZeneca requests authorization from the European Medicines Agency for its vaccine - The Gal Times
The Oxford / AstraZeneca vaccine does not use the revolutionary genetic technology of mRNA or messenger RNA like Pfizer and Moderna, but the more traditional virus-vector type that takes as a carrier another virus (a chimpanzee adenovirus) although genetically modified and adapted to combat Covid-19.. However, it has been criticized due to the confusion in the interim results of clinical trials. The British laboratory announced in November that its vaccine had an average efficacy of 70%, compared to more than 90% for the Pfizer / BioNTech and Moderna vaccines.. The efficacy of the AstraZeneca / Oxford vaccine was 90% for volunteers who received a half dose first and then a full dose one month later, but only 62% for another group that was more frequently vaccinated with two full doses with a month difference.. The half-dose injection was, in fact, due to an error and only a small group had followed the second protocol, which had raised concern, and led the company to announce on November 26 ...
Mastadenovirus (genus), human adenovirus (species). The GMO is a replication incompetent recombinant adenovirus (human serotype 5) containing the human gene for interleukin-12 (referred to as Adv/IL-12) ...
Effect of elapsed time between BCG priming and AdHu5Ag8 | Open-i
Effect of elapsed time between BCG priming and AdHu5Ag85A boost immunization on Ag-specific responses boosted by AdHu5Ag85A boost immunization.Infant and adult
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Search of: 15128818 [PUBMED-IDS] - List Results - ClinicalTrials.gov
Magnitude and frequency of immune responses between the adenoviral vaccines following DNA prime, assessed by enzyme-linked immunospot (ELISpot) responses, intracellular cytokine staining (ICS) T-cell responses, and HIV-1 antibody ...
Evaluation of the Efficacy of ChAd63-MVA Vectored Vaccines Expressing Circumsporozoite Protein and ME-TRAP Against Controlled...
BACKGROUND: Circumsporozoite protein (CS) is the antigenic target for RTS,S, the most advanced malaria vaccine to date. Heterologous prime-boost with the viral vectors simian adenovirus 63 (ChAd63)-modified vaccinia virus Ankara (MVA) is the most potent inducer of T-cells in humans, demonstrating significant efficacy when expressing the preerythrocytic antigen insert multiple epitope-thrombospondin-related adhesion protein (ME-TRAP). We hypothesized that ChAd63-MVA containing CS may result in a significant clinical protective efficacy. METHODS: We conducted an open-label, 2-site, partially randomized Plasmodium falciparum sporozoite controlled human malaria infection (CHMI) study to compare the clinical efficacy of ChAd63-MVA CS with ChAd63-MVA ME-TRAP. RESULTS: One of 15 vaccinees (7%) receiving ChAd63-MVA CS and 2 of 15 (13%) receiving ChAd63-MVA ME-TRAP achieved sterile protection after CHMI. Three of 15 vaccinees (20%) receiving ChAd63-MVA CS and 5 of 15 (33%) receiving ChAd63-MVA ME-TRAP
Benkő, Mária Dr. | MTA ÁOTI
Podgorski II, Pantó L, Papp T, Harrach B Benkő M (2016) Genome analysis of four Old World monkey adenoviruses supports the proposed species classification of primate adenoviruses and reveals signs of possible homologous recombination. J Gen Virol. [Epub ahead of print] doi: 10.1099/jgv.0.000465. Pénzes JJ, Pham HT, Benkő M, Tijssen P (2015) Novel parvoviruses in reptiles and genome sequence of a lizard parvovirus shed light on Dependoparvovirus genus evolution. J Gen Virol. 96:2569-2579. doi: 10.1099/vir.0.000215. Doszpoly A, Tarján LZ, Glávits R, Müller T, Benkő M (2014) Full genome sequence of a novel circo-like virus detected in an adult European eel Anguilla anguilla showing signs of cauliflower disease. Dis Aquat Organ. 109:107-115. doi: 10.3354/dao02730. Kaján GL, Kecskeméti S, Harrach B, Benkő M (2013) Molecular typing of fowl adenoviruses, isolated in Hungary recently, reveals high diversity. Vet Microbiol. 167:357-363. doi: 10.1016/j.vetmic.2013.09.025. Doszpoly A, Wellehan ...
A Novel Vaccine Strategy Employing Serologically Different Chimpanzee Adenoviral Vectors for the Prevention of HIV-1 and HCV...
Background: Nearly 3 million people worldwide are coinfected with HIV and HCV. Affordable strategies for prevention are needed. We developed a novel vaccination regimen involving replication-defective and serologically distinct chimpanzee adenovirus (ChAd3, ChAd63) vector priming followed by modified vaccinia Ankara (MVA) boosts, for simultaneous delivery of HCV non-structural (NSmut) and HIV-1 conserved (HIVconsv) region immunogens. Methods: We conducted a phase I trial in which 33 healthy volunteers were sequentially enrolled and vaccinated via the intramuscular route as follows: 9 received ChAd3-NSmut [2.5 × 1010 vp] and MVA-NSmut [2 × 108 pfu] at weeks 0 and 8, respectively; 8 received ChAdV63.HIVconsv [5 × 1010 vp] and MVA.HIVconsv [2 × 108 pfu] at the same interval; 16 were co-primed with ChAd3-NSmut [2.5 × 1010 vp] and ChAdV63.HIVconsv [5 × 1010 vp] followed at week 8 by MVA-NSmut and MVA.HIVconsv [both 1 × 108 pfu]. Immunogenicity was assessed using peptide pools in ex vivo ELISpot and
Frontiers | Assessment of Antibodies Induced by Multivalent Transmission-Blocking Malaria Vaccines | Immunology
A malaria transmission-blocking vaccine would be a critical tool in achieving malaria elimination and eradication. Using chimpanzee adenovirus serotype 63 and modified vaccinia virus Ankara viral vectored vaccines, we investigated whether incorporating two antigens into one vaccine would result in higher transmission-reducing activity than one antigen. We demonstrated that when Pfs25 was administered with other antigens Pfs28 or Pfs230C, either concurrently, as a mixed vaccine, or co-expressed as a dual-antigen vaccine, the antibody response in mice to each antigen was comparable to a mono-antigen vaccine, without immunological interference. However, we found that the transmission-reducing activity (functional activity) of dual-antigen vaccines was not additive. Dual-antigen vaccines generally only elicited similar transmission-reducing activity to mono-antigen vaccines, and in one instance had lower transmission-reducing activity. We found that despite the lack of immunological interference of dual
Vaccine Carrier 8Liter OneMed - OneMed-Medicom
Box yang digunakan untuk menyimpan vaksin dalam suhu dingin untuk sementara waktu ketika akan dikirim ke tempat yang lain. Cocok untuk dokter hewan dan perlindungan botani. Volume 8 Liter dilengkapi dengan 6 ice brick. Dilengkapi dengan pengunci, handle dan thermometer suhu dalam box. Merk OneMed Spesifikasi: Type : HT
Immunogenicity and Efficacy of Zika Virus Envelope Domain III in DNA, Protein, and ChAdOx1 Adenoviral-Vectored Vaccines. -...
The flavivirus envelope protein domain III (EDIII) was an effective immunogen against dengue virus (DENV) and other related flaviviruses. Whether this can be applied to the Zika virus (ZIKV) vaccinology remains an open question. Here, we tested the efficacy of ZIKV-EDIII against ZIKV infection, using several vaccine platforms that present the antigen in various ways. We provide data demonstrating that mice vaccinated with a ZIKV-EDIII as DNA or protein-based vaccines failed to raise fully neutralizing antibodies and did not control viremia, following a ZIKV challenge, despite eliciting robust antibody responses. Furthermore, we showed that ZIKV-EDIII encoded in replication-deficient Chimpanzee adenovirus (ChAdOx1-EDIII) elicited anti-ZIKV envelope antibodies in vaccinated mice but also provided limited protection against ZIKV in two physiologically different mouse challenge models. Taken together, our data indicate that contrary to what was shown for other flaviviruses like the dengue virus, which has
Isotype Control (Igs)-Genemed Synthesis Inc.
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Recombinant Human CHAD Protein best suppliers/vendors - Labshake
Recombinant Human CHAD Protein best suppliers; Recombinant Human CHAD Protein best sources; Recombinant Human CHAD Protein best vendors; Recombinant Human CHAD Protein protocol; Recombinant Human CHAD Protein citations; Recombinant Human CHAD Protein publications; Recombinant Human CHAD Protein papers - Labshake
A Human Type 5 Adenovirus-Based Tuberculosis Vaccine Induces Robust T Cell Responses in Humans Despite Preexisting Anti...
There is an urgent need to develop new tuberculosis (TB) vaccines to safely and effectively boost Bacille Calmette-Guérin (BCG)-triggered T cell immunity in humans. AdHu5Ag85A is a recombinant human type 5 adenovirus (AdHu5)-based TB vaccine with demonstrated efficacy in a number of animal species, yet it remains to be translated to human applications. In this phase 1 study, we evaluated the safety and immunogenicity of AdHu5Ag85A in both BCG-naïve and previously BCG-immunized healthy adults. Intramuscular immunization of AdHu5Ag85A was safe and well tolerated in both trial volunteer groups. Moreover, although AdHu5Ag85A was immunogenic in both trial volunteer groups, it much more potently boosted polyfunctional CD4+ and CD8+ T cell immunity in previously BCG-vaccinated volunteers. Furthermore, despite prevalent preexisting anti-AdHu5 humoral immunity in most of the trial volunteers, we found little evidence that such preexisting anti-AdHu5 immunity significantly dampened the potency of ...
Items where Author is Benkő, Mária - Repository of the Academys Library
Marek, Ana and Kaján, Győző and Kosiol, Carolin and Benkő, Mária and Schachner, Anna and Hess, Michael (2016) Genetic diversity of species Fowl aviadenovirus D and Fowl aviadenovirus E. Journal of General Virology, 97. pp. 2323-2332. ISSN 0022-1317 (print), 1465-2099 (online) Pénzes, Judt J. and Pham, Hanh T. and Benkő, Mária and Tijssen, Peter (2015) Novel parvoviruses in reptiles and genome sequence of a lizard parvovirus shed light on Dependoparvovirus genus evolution. Journal of General Virology, 96 (9). pp. 2569-2579. ISSN 0022-1317 (print), 1465-2099 (online) Pénzes, Judt J. and Menendez-Conejero, R. and Condezo, G.N. and Ball, Ina and Papp, Tibor and Doszpoly, Andor and Paradela, A and Perez-Berna, A.J. and Lopez-Sans, M and Nguyen, T. H. and van Raaij, M.J. and Marschang, R.E. and Harrach, Balázs and Benkő, Mária and San Martin, C (2014) Molecular characterization of a lizard adenovirus reveals the first atadenovirus with two fiber genes and the first adenovirus with either ...
Chad E Exner Mugshot - Chad E Exner Arrest - Kenosha County, WI
Chad E Exner Mugshot - Find Latest Inmate Mugshots online, Search Latest Arrested Inmate Records, Newest Booking Records , Buck E Exner, Charlotte E Exner, Charlie E Exner, Charles E Exner, Chuck E Exner, Carl E Exner, Chick E Exner, Caroline E Exner
Steve Chen & Chad Hurley - Variety
Fourteen months after it was acquired by Google, the seminal video sharing website Chen and Hurley started in February 2005 is more relevant than ever, its unique users and page views spiking 83% and 200% respectively from September 2006 to September 2007.
Structural and functional annotation of hypothetical proteins of human adenovirus: prioritizing the novel drug targets | BMC...
Human adenoviruses are non-enveloped dsDNA viruses of almost 35 kb in size . HAdV can infect a variety of tissues and cause a wide range of complications like gastroenteritis, hepatitis, myocarditis, keratoconjunctivitis and pneumonia [2, 3]. It is contagion in nature which occurs through direct contact or fomites and virus is also resistant to various physical and chemical agents. Children younger than the age of 5 years and immune compromised persons especially the pediatric patients are most susceptible to these viruses. Worldwide 5-7% respiratory tract infections are ascribed by HAdV in pediatric patients  and persons of all ages are susceptible to infections caused by these viruses .. Seven known Human adenoviruses species from HAdV-A to HAdV-G are constitute of the genus Mastadenovirus in which all the human adenoviruses are categorized and further divided into different strains . Now 67 types of HAdV have been reported . Their number is rapidly increasing due to ...
Functional characterization of the human adenovirus pVII protein and non-coding VA RNAI
Human adenovirus (HAdV) is a common pathogen causing a broad spectrum of diseases. HAdV encodes the pVII protein, which is involved in nuclear delivery, protection and expression of viral DNA. To suppress the cellular interferon (IFN) and RNA interference (RNAi) systems, HAdVs encode non-coding virus-associated (VA) RNAs. In this thesis we have investigated the functional significance of the pVII protein and VA RNAI in HAdV-5 infected cells.. We report that the propeptide module is the destabilizing element targeting the precursor pVII protein for proteasomal degradation. We also found that the Cul3-based E3 ubiquitin ligase complex alter the precursor pVII protein stability via binding to the propeptide sequence. In addition, we show that inhibition of the Cul3 protein reduces HAdV-5 E1A gene expression. Collectively, our results suggest a novel function for the pVII propeptide module and involvement of Cul3 in viral E1A gene expression.. Our studies show that the cellular E3 ubiquitin ligase ...
Submit Comment for Designer Vaccine Carrier Protein Complex
Are you concerned about Air Pollution in your area?. Maybe modelling air pollution will get you the answers you need for this problem.. Thats what I do full-time. Try it.. ...
Lee D Chad Brock Colin S from Bareback That Hole at JustUsBoys - Gallery 26820
Lee D Chad Brock Colin S (Bareback That Hole) - Lee Denim, Chad Brock, and Colin Steele?! In one hot daddy dick and ass bareback flip fucking tag team!
Chad Grey - Everything2.com
Chad Grey is the lead vocalist for the American heavy metal band MuDvAyNe. He, along with the other members of the band foolishly preformed a satanic ri...
Hari Chad on WN Network delivers the latest Videos and Editable pages for News & Events, including Entertainment, Music, Sports, Science and more, Sign up and share your playlists.
Chad Gray Says Hellyeah Will Hopefully Return With New Music: We Need To Get Together And Write - Blabbermouth.net
During a December 5-6 Headbangers Con live virtual panel, HELLYEAH frontman Chad Gray was asked if the band will keep going once the pandemic has s...
Ribosomal proteins in normal simian cells, sv40-transformed simian cells, and simian cells infected with sv40, adenovirus 5,...
TY - JOUR. T1 - Ribosomal proteins in normal simian cells, sv40-transformed simian cells, and simian cells infected with sv40, adenovirus 5, and vesicular stomatitis virus. AU - Bosselman, Robert A.. AU - Price, Joseph A.. AU - Lee Burns, A.. AU - Kaulenas, Mindaugas S.. AU - Norkin, Leonard C.. PY - 1978/1/1. Y1 - 1978/1/1. N2 - The protein patterns of monosomes and polysomes isolated from the T-22 line of SV40-transformed GMK cells and from uninfected CV-I cells and CV-1 cells infected with SV40, adenovirus 5, or vesicular stomatitis virus were analyzed by two-dimensional PAGE. All gel patterns were similar except for the presence of one additional protein associated with T-22 monosomes.. AB - The protein patterns of monosomes and polysomes isolated from the T-22 line of SV40-transformed GMK cells and from uninfected CV-I cells and CV-1 cells infected with SV40, adenovirus 5, or vesicular stomatitis virus were analyzed by two-dimensional PAGE. All gel patterns were similar except for the ...
Endosomophagy clears disrupted early endosomes but not virus particles during virus entry into cells - Matters
Enveloped viruses fuse with host membranes without affecting cell integrity. Non-enveloped viruses and bacteria penetrate by rupturing endosomal membranes and thus expose complex-type carbohydrates from the endosome lumen to cytosolic proteins. Here we report on the dynamics and initial marker analyses of Galectin-3 (Gal3)-positive membranes triggered by incoming adenovirus species B/C in HeLa cells. Using mCherry-Gal3 reporter constructs, immunolabeling, confocal and electron microscopy, we detected robust signals from Gal3-containing, early endosomal antigen 1-positive membranes 1 h post-infection (pi). Adenoviruses penetrate from non-acidic endosomes with high efficiency, 15 min pi, and largely outnumbered the Gal3-positive membranes, suggesting that Gal3 recruitment to broken membranes is transient, or Gal3-positive membranes are rapidly turned-over. In support of rapid turn-over, Gal3 was found within single-membrane vesicles and degradative autophagosomes. The Gal3 membranes contained ubiquitin
Chad Tilbury | SANS Senior Instructor
View Chad Tilburys bio. Chad will be teaching Advanced Digital Forensics, Incident Response, and Threat Hunting, Windows Forensic Analysis, Advanced Digital Forensics, Incident Response, and Threat Hunting.
Chad Lowndes - Honolulu, HI - Counseling | Healthgrades.com
Visit Healthgrades for information on Chad Lowndes Find Phone & Address information, medical practice history, affiliated hospitals and more.
The FAO Component of the Consolidated Appeals 2009: Chad : La FAO en situaciones de emergencia
The FAO Component of the Consolidated Appeals Process (CAP) 2009 for Chad. A joint effort to plan, coordinate and monitor the response to complex emergencies.
Chad: Clearly The Most Likely To Be Fabulous [Pic] - i am bored
Any one else see the soon to be serial killer in the middle next to the young spock with the look on his face that is proably what he will grow into and I bet his name is Dexter also ...
Masked 2, Chad McCail - CultureLabel
I make drawings, paintings and prints working in a figurative, illustrative manner. There should be no question as to what is represented but that the viewers
Species-Specific Identification of Human Adenoviruses in Sewage | PJM ONLINE
Albinana-Gimenez N., M.P. Miagostovich, B. Calgua, J.M. Huguet, L. Matia and R. Girones. 2009. Analysis of adenoviruses and polyomaviruses quantified by qPCR as indicators of water quality in source and drinking-water treatment plants. Water Res. 43:2011-2019. [PubMed]. Barrella K.M., P. Garrafa, T.A. Monezi, C.M. Harsi, C. Salvi, P.A. Violante and D.U. Mehnert. 2009. Longitudinal study on occurrence of adenoviruses and hepatitis A virus in raw domestic sewage in the city of Limeira, Sao Paulo. Braz. J. Microbiol. 40:102-107. [PubMed]. Bibby K. and J. Peccia. 2013. Prevalence of respiratory adenovirus species B and C in sewage sludge. Environ. Sci. Process Impacts. 15(2):336-8 [PubMed]. Cantalupo P.G, B. Calgua, G. Zhao, A. Hundesa, A.D. Wier, J.P. Katz, M. Grabe, R.W. Hendrix, R. Girones, D. Wang and others. 2011. Raw sewage harbors diverse viral populations. MBio. 2(5):e00180-11 [PubMed]. Cruz J.R., P. Caceres, F. Cano, J. Flores, A. Bartlett and B. Torun. 1990. Adenovirus types 40 and 41 and ...
Chad Health, Chad Medical Information, Vaccinations for Chad, Malaria in Chad, Travel Guide
Chad Health: Protect your health in to Chad. Our travel health guide for Chad gives helpful medical information on vaccinations, malaria, and other medical issues for Chad travel.
UNESCO Sites: An Opportunity for Chad | DW Travel | DW | 04.07.2017
Tourism is minimal in Chad, one of Africas poorest countries. Two areas have recently been designated UNESCO World Heritage sites. We ask desert researcher Stefan Kröpelin how that status can benefit Chad.
Search results for Chad
Chad Cromwell Chad Cromwell, Drummer, Mark Knopfler, Got Any Gum?, Freedom (Neil Young album), Prairie Wind. Log Press (2011-01-30) - ISBN-13: 978-613-4-93039-0 ...
Chad Eubank: All Heart
It takes 8 seconds to make a champion in the world of bull riding. However, it took cowboy Chad Eubank years before he realized that he was already a winner in Gods eyes.
Chad and the EU - European External Action Service
Chad is one of the poorest countries in the world. It receives European Union (EU) aid in the political, economic and commercial domains, as well as development aid and humanitarian aid.
Hydrohalogenation Addition to Alkenes - Chads Prep®
Chad breaks down Hydrohalogation Addition to Alkenes (including the mechanism) including why it is Markovnikov addition with no Stereospecificty.
Chad Ochocinco misses Patriots practice
By Shalise Manza Young, Globe Staff When the Patriots gathered on the fields behind Gillette Stadium for Thursdays organized team activity session,
Rotavirus - Articles on Medical Diseases and Conditions
These DNA viruses (unlike other adenoviruses) could not be cultured using standard virus culture systems. The most frequent are types 40 and 41. These enteric adenovirus species are the second most common cause of severe gastroenteritis in young children (after rotavirus), comprising about 10%-15% of cases (range, 5%-52%). It has also been the second or third most common overall cause of infant gastroenteritis. Diarrhea is the predominant symptom. Vomiting may be present but is less prominent than that seen in rotavirus infection. The gold standard for diagnosis has been EM of stool specimens. However, EM cannot differentiate between fastidious and other adenovirus species. Culture can be done in some cases using nonroutine tissue culture cells. Several EIA tests for virus antigen in stool have been described and one commercial kit for types 40 and 41 is now available. Nucleic acid probes have also been used experimentally.. ...
The Use of Nanobot Technology (NT) & Artificial Intelligence (AI) as a Vaccine Carrier and Adjuvant
Programmable magnetic nanobots injected into the vascular and interstitial fluids of a human or animal body are found to be deposited into the connective and fatty tissues and then into the organs and glands potentially causing biological transformation of the cell membrane (crowning and protein spiking), genetic mutation, and the death of the cell. Nanobots can be injected using an ordinary hypodermic syringe. The nanobots are microscopic functioning robots with the ability to walk and wi
Human adenovirus A serotype 12 (HAdV-12) (Human adenovirus 12)
Your basket is currently empty. i ,p>When browsing through different UniProt proteins, you can use the basket to save them, so that you can back to find or analyse them later.,p>,a href=/help/basket target=_top>More...,/a>,/p> ...
Chad Seabold , DDS | Dentist in 77505
DentalPlans detailed profile of Chad Seabold , DDS - Dentist in 77505. View plans, sample savings & pricing, patient reviews & practice information.
Chad Morse | Esalen
Chad Morse is an educator and farmer. He managed the Esalen Farm for 4 years, co-led the Farm & Garden apprenticeship program, and has a background in cross-cultural and service-based education.
Throwback: Chad Vaughn 190kg American Record Clean & Jerk
Its still the standing American record: Chad Vaughns 190 kilogram clean & jerk in the 77kg weight class, set during the 2007 Arnold Classic.
Chad Crowley, Racial Realities | Counter-Currents Publishing
2,947 words Contrary to conventional wisdom, race isnt a concept, construct, or any other type of trite postmodern abstraction, but rather a scientifically
Simian adenoviruses do not suffer from the same disadvantages. Therefore, investigators have tested new vaccines using the ... ChAdOx1 has been derived from a simian adenovirus (ChAd) serotype Y25 engineered by λ red recombination to exchange the native ... Morris, Susan J; Sebastian, Sarah; Spencer, Alexandra J; Gilbert, Sarah C (1 September 2016). "Simian adenoviruses as vaccine ... The vector is a chimpanzee adenovirus modified to avoid its replication. Adenoviruses are effective vectors for inducing and ...
Kidd, AH; Garwicz D; Oberg M (1995). "Human and simian adenoviruses: phylogenetic inferences from analysis of VA RNA genes". ... These two VA RNA genes are distinct genes in the adenovirus genome. VA RNAI is the major species with VA RNAII expressed at a ... The VA (viral associated) RNA is a type of non-coding RNA found in adenovirus. It plays a role in regulating translation. There ... Ma, Y; Mathews MB (1996). "Secondary and tertiary structure in the central domain of adenovirus type 2 VA RNA I". RNA. 2 (9): ...
Yamamoto, Hiroshi; Shimojo, H (August 1971). "Multiplicity reactivation of human adenovirus type 12 and simian virus 40 ... SV40 is an abbreviation for simian vacuolating virus 40 or simian virus 40, a polyomavirus that is found in both monkeys and ... Barbanti-Brodano, G; Sabbioni, S; Martini, F; Negrini, M; Corallini, A; Tognon, M (2004). "Simian virus 40 infection in humans ... NIH/National Cancer Institute (25 August 2004). "Studies Find No Evidence That Simian Virus 40 Is Related To Human Cancer". ...
Thomas J. Kelly (scientist)
Kelly, T J; Rose, J A (1971). "Simian virus 40 integration site in an adenovirus 7-simian virus 40 hybrid DNA molecule". ... adenovirus and SV40, which cause tumors in animals. He joined the faculty in the Department of Microbiology at the Johns ... "Replication of adenovirus and SV40 chromosomes in vitro". Phil. Trans. R. Soc. Lond. B. 317 (1187): 429-438. Bibcode:1987RSPTB. ... "The DNA-activated protein kinase is required for the phosphorylation of replication protein A during simian virus 40 DNA ...
The adenovirus E1B protein (55K) prevents p53 from regulating genes by binding to the site on p53 which binds to the genome. In ... Simian virus 40 (SV40), a polyomavirus, can cause tumors in rodent models but is not oncogenic in humans. This phenomenon has ... Adenoviruses can lead to tumors in rodent models but do not cause cancer in humans; however, they have been exploited as ... The adenovirus early region 1A (E1A) is an oncoprotein which binds to Rb and can stimulate transcription and transform cells. ...
Schuitemaker demonstrated the efficacy of the adenovirus/protein vaccines against simian immunodeficiency virus in rhesus ... "Protective efficacy of adenovirus/protein vaccines against SIV challenges in rhesus monkeys". Science. 349 (6245): 320-324. ...
SV40, a enciclopedia libre
Yamamoto, Hiroshi; Shimojo, H (August 1971). "Multiplicity reactivation of human adenovirus type 12 and simian virus 40 ... O SV40, do inglés simian vacuolating virus 40 ou simian virus 40 (virus simio vacuolante 40 ou virus simio 40), é un ... Simian Virus 40 (SV40:) A Possible Human Polyomavirus Workshop Monday January 27, 1997, Morning Session, transcript of 1997 ... Simian Virus 40 (SV40:) A Possible Human Polyomavirus Workshop Monday January 27, 1997 Afternoon Session, transcript of 1997 ...
Helper dependent virus
... which was originally discovered as a contaminant in a sample of simian adenovirus. Though AAV is considered to be dependent on ... Alba, R; Bosch, A; Chillon, M (2005). "Gutless adenovirus: Last-generation adenovirus for gene therapy". Gene Therapy. 12: S18- ... 1965). "Adenovirus-Associated Defective Virus Particles". Science. 149 (3685): 754-6. Bibcode:1965Sci...149..754A. doi:10.1126/ ... "Production of high-titer recombinant adeno-associated virus vectors in the absence of helper adenovirus". Journal of Virology. ...
A number of vectors based on other type of viruses like Adenoviruses and Papilloma virus have been used to clone genes in ... A vector based on Simian virus 40 (SV40) was used in first cloning experiment involving mammalian cells. ...
"Protective efficacy of a novel simian adenovirus vaccine against lethal MERS-CoV challenge in a transgenic human DPP4 mouse ... "Chimpanzee Adenovirus Vaccine Provides Multispecies Protection against Rift Valley Fever". Scientific Reports. 6 (1): 20617. ...
Simian foamy viruses (SFV) is an enzootic retrovirus that has high rates of cross-species transmission and has been known to ... Diseases such as HIV and human adenoviruses have been associated with NHP interactions. In places where contact between humans ... titi monkey adenovirus) is a highly divergent, sharing ...
Eugene O. Major
Throughout his career, Major has conducted research on viruses including BK virus, adenoviruses, JC virus, simian virus 40 ( ... Major, E. O.; Matsumura, P. (1984). "Human embryonic kidney cells: stable transformation with an origin-defective simian virus ... Bourne, KA; Major, EO; Khoobyarian, N (1978). "A variant of adenovirus 12 producing cytoplasmic accumulation of capsid proteins ...
Bruce William Stillman
... starting with studying DNA replication of human adenovirus as a model. He then began to study how the genome of simian virus 40 ...
California National Primate Research Center
The research center was the site of this outbreak in what is being considered the first known case of an adenovirus jumping ... There is a growing evidence of the contribution of CNPRC to the origins of simian immunodeficiency virus (SIV). Evidence from ... In 2009 an outbreak of a monkey-killing cold virus identified as an adenovirus infected both monkeys and humans, with the ... Mansfield, K. G., N. W. Lerche, M. B. Gardner, and A. A. Lackner (May 1995). "Origins of simian immunodeficiency virus ...
Multiplicity reactivation has been found to occur with pathogenic viruses including influenza virus, HIV-1, adenovirus simian ...
serotype 14: can cause potentially fatal adenovirus infections. Canine adenovirus 1 (CAdV-1) can lead to death in puppies, or ... Ovine mastadenovirus A Ovine mastadenovirus B Porcine mastadenovirus A Porcine mastadenovirus B Porcine mastadenovirus C Simian ...
The agent was later identified as a monkey virus and named Simian Virus 40 (SV40). Sweet & Hilleman published their work in ... Hilleman at Merck observed a similar agent in the same kind of medium that they were using to develop a vaccine for adenovirus ... Maryland to attend a workshop of the National Institutes of Health entitled Simian Virus 40: A Possible Human Polyomavirus. ...
List of MeSH codes (B04)
... adenoviruses, human MeSH B04.280.030.500.675 - adenoviruses, porcine MeSH B04.280.030.500.700 - adenoviruses, simian MeSH ... adenoviruses, human MeSH B04.909.204.097.500.675 - adenoviruses, porcine MeSH B04.909.204.097.500.700 - adenoviruses, simian ... simian immunodeficiency virus MeSH B04.820.650.805.851 - simian t-lymphotropic virus 1 MeSH B04.820.650.805.855 - simian t- ... simian t-lymphotropic virus 1 MeSH B04.909.777.731.805.855 - simian t-lymphotropic virus 2 MeSH B04.909.777.731.850 - ...
Oxford-AstraZeneca COVID-19 vaccine
The Oxford-AstraZeneca COVID-19 vaccine is a replication-deficient simian adenovirus vector, containing the full‐length codon‐ ... The adenovirus is called replication-deficient because some of its essential genes were deleted and replaced by a gene coding ... Following vaccination, the adenovirus vector enters the cells and releases its genes, which are transported to the cell nucleus ... 2012). "A novel chimpanzee adenovirus vector with low human seroprevalence: improved systems for vector derivation and ...
... a simian adenovirus used to develop ChAdOx1 Chad-e Bala or Upper Chad, a village in Iran Chad-e Pain or Lower Chad, a village ...
Ginsberg showed the process by which the adenovirus caused disease after entering host cells, leading to the creation of ... At the National Institute of Allergy and Infectious Diseases, Ginsberg performed research on Simian immunodeficiency virus, a ... He was on the faculty of Western Reserve University starting in 1951, where research he conducted showed that adenoviruses, ... "he had accomplished all he could with adenoviruses" before moving on to study HIV. A resident of both Woods Hole, Massachusetts ...
Peter M. Howley
1991 Chellappan, S.; Kraus, V. B.; Kroger, B.; Munger, K.; Howley, P. M.; Phelps, W. C.; Nevins, J. R. (1992). "Adenovirus E1A ... simian virus 40 tumor antigen, and human papillomavirus E7 protein share the capacity to disrupt the interaction between ...
Herpes simplex virus
... adenovirus simian virus 40, vaccinia virus, reovirus, poliovirus and herpes simplex virus. When HSV particles are exposed to ...
Whyte, P (1988). "Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma ... "Monoclonal antibodies specific for simian virus 40 tumor antigens." Journal of Virology 39.3 (1981): 861-869. Tsai, Li-Huei, et ... "Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma gene product." ( ... Harlow, E (1981). "Monoclonal antibodies specific for simian virus 40 tumor antigens". J Virol. 39: 861-9. PMC 171319. PMID ...
Oxford-AstraZeneca COVID-19 vaccine
The Oxford-AstraZeneca COVID-19 vaccine is a replication-deficient simian adenovirus vector, containing the full‐length codon‐ ... The adenovirus is called replication-deficient because some of its essential genes were deleted and replaced by a gene coding ... Following vaccination, the adenovirus vector enters the cells and releases its genes, which are transported to the cell nucleus ... using as a vector the modified chimpanzee adenovirus ChAdOx1. The efficacy of the vaccine is 76.0% at ...
... is an adenovirus protein of 14kDa which regulates growth in all stages of the adenovirus (Ad) infection. E4orf4 partners mainly ... Toolan, H.W. and Ledinko, N. (1965) Growth and cytopathogenicity of H-viruses in human and simian cell cultures. Nature 208, 8 ... It was seen in one Phase I clinical trial that injecting mda-7 via an adenovirus directly into a tumor resulted in safe tumor ... Toolan, H.W. and Ledinko, N. (1968) Inhibition by H-l virus of the incidence of tumors produced by adenovirus 12 in hamsters. ...
HEK 293 cells
... and could be preferentially transformed by adenovirus. Adenoviruses transform neuronal lineage cells much more efficiently than ... Stillman BW, Gluzman Y (August 1985). "Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells". ... The cells were cultured by van der Eb; the transduction by adenovirus was performed by Frank Graham, a post-doc in van der Eb's ... However, the original adenovirus transformation was inefficient, suggesting that the cell that finally produced the HEK 293 ...
Yamamoto H, Shimojo H (August 1971). "Multiplicity reactivation of human adenovirus type 12 and simian virus 40 irradiated by ... Adenoviruses are also known to cause respiratory infections in horses, cattle, pigs, sheep, and goats. Equine adenovirus 1 can ... Main article: Adenovirus vaccine. In the past, US military recruits were vaccinated against two serotypes of adenovirus, with a ... In babies, adenoviruses can also cause coughing fits that look almost exactly like whooping cough. Adenoviruses can also cause ...
The adenovirus E1B protein (55K) prevents p53 from regulating genes by binding to the site on p53 which binds to the genome.[32 ... 1961: Simian Vacuolating virus 40 (SV40) discovered by Eddy at NIH, and Hillman and Sweet at Merck laboratory as a rhesus ... The adenovirus early region 1A (E1A) is an oncoprotein which binds to Rb and can stimulate transcription and transform cells.[ ... Adenoviruses can lead to tumors in rodent models but do not cause cancer in humans; however, they have been exploited as ...
Virus, den frie encyklopædi
Model af adenovirus-kapsid, prototypen på et kapsid af en dsDNA-virus ... model af Simian immunodeficiency virus, SIV, prototypen på en revers transkriberende RNA-virus ...
... mice by helper-dependent adenovirus-based expression of gulonolactone oxidase". Hum. Gene Ther. 19 (12): 1349-58. doi:10.1089/ ... include the tarsiers and the simians (apes, monkeys and humans). The strepsirrhines (bent or wet-nosed) primates, which are ...
The foremost issue facing the rAd5 adenovirus that was used is the high prevalence of the adenovirus-specific antibodies as a ... Kim PS, Malashkevich VN, Chan DC, Chutkowski CT (1998). "Crystal structure of the simian immunodeficiency virus (SIV) gp41 core ... Adenoviruses are among the main causes of upper respiratory tract ailments such as the common cold. Because the vaccine ... Adenovirus vectors and many other viral vectors currently used in HIV vaccines, will induce a rapid memory immune response ...
Head and neck cancer
Gendicine is a gene therapy that employs an adenovirus to deliver the tumor suppressor gene p53 to cells. It was approved in ... and 8-oxo-purines in simian kidney cells". Nucleic Acids Res. 34 (8): 2305-15. doi:10.1093/nar/gkl099. PMC 1458282 . PMID ...
Marburg virus disease
Martini, G. A.; Schmidt, H. A. (1968). "Spermatogenic transmission of the "Marburg virus". (Causes of "Marburg simian disease ... "Complex adenovirus-vectored vaccine protects guinea pigs from three strains of Marburg virus challenges". Virology. 353 (2): ...
Suzuki Y (2006). "Ancient positive selection on CD155 as a possible cause for susceptibility to poliovirus infection in simians ... Adenovirus Adenovirus infection. RNA virus. Rotavirus. Norovirus. Astrovirus. Coronavirus. Hepatitis. DNA virus. HBV (B). RNA ...
Hilleman was one of the early vaccine pioneers to warn about the possibility that simian viruses might contaminate vaccines. ... He also played a role in the discovery of the cold-producing adenoviruses, the hepatitis viruses, and the cancer-causing virus ...
... adenovirus, HIV, HTLV, hepatitis B, hepatitis C, parvovirus B19, and influenza virus). Of possible relevance to transfusion ... "Pathogenic simian immunodeficiency virus infection is associated with expansion of the enteric virome". Cell. 151 (2): 253-66. ... adenoviruses, anelloviruses, parvoviruses, and circoviruses. Each individual had a distinct viral profile, demonstrating the ...
Family with sequence similarity 111 member a
... consistent with the demonstrated role for this gene in Simian Virus 40 (SV40) viral replication. Mutations in this gene have ... "Identification of FAM111A as an SV40 host range restriction and adenovirus helper factor". PLOS Pathog. 8 (10): e1002949. doi: ...
Yamamoto H, Shimojo H (August 1971). "Multiplicity reactivation of human adenovirus type 12 and simian virus 40 irradiated by ... Adenoviruses Stanford University-Adenoviruses Adenoviruses General Concepts General information on Adenovirus DNA virus ... Tupaia adenovirus (TAV) (tree shrew adenovirus 1) has been isolated from tree shrews. Otarine adenovirus 1 has been isolated ... In babies, adenoviruses can also cause coughing fits that look almost exactly like whooping cough. Adenoviruses can also cause ...
Genetically modified virus
Viruses that have been used for gene therapy are, adenovirus, lentivirus, retrovirus and the herpes simplex virus.The most ... Jackson DA, Symons RH, Berg P (October 1972). "Biochemical method for inserting new genetic information into DNA of Simian ... such as adenovirus, and modify its genome to have genes that code for immunogenic proteins that can spike the immune systems ... "Oncolytic adenovirus modified with somatostatin motifs for selective infection of neuroendocrine tumor cells". Gene Therapy. 18 ...
Transporter Classification Database
Family 1.A.103 The Simian Virus 5 (Parainfluenza Virus 5) SH (SV5-SH) Family 1.A.104 The Proposed Flagellar Biosynthesis Na+ ... envelope anion-channel-forming Tic110 family 1.A.19 Type A influenza virus matrix-2 channel family 1.A.20 BCL2/Adenovirus E1B- ...
SV40 large T antigen
... (Simian Vacuolating Virus 40 TAg) is a hexamer protein that is a dominant-acting oncoprotein derived from ... SV40 large TAg, other polyomavirus large T antigens, adenovirus E1a proteins, and oncogenic human papillomavirus E7 proteins ... Chen S, Paucha E (July 1990). "Identification of a region of simian virus 40 large T antigen required for cell transformation ...
PLOS Neglected Tropical Diseases: A high infectious simian adenovirus type 23 vector based vaccine efficiently protects common...
A simian-adenovirus-vectored rabies vaccine suitable for thermostabilisation and clinical development for low-cost single-dose...
Prevention of Primary Simian Adenovirus Type 7 (SA7) Tumors in Hamsters by Adoptive Transfer of Lymphoid Cells: Role of...
Immunogenicity and efficacy of the novel cancer vaccine based on simian adenovirus and MVA vectors alone and in combination...
The simian adenovirus, ChAdOx1, and modified vaccinia Ankara virus, MVA, encoding a prostate cancer-associated antigen, the six ... Immunogenicity and efficacy of the novel cancer vaccine based on simian adenovirus and MVA vectors alone and in combination ... More News: Adenoviruses , Allergy & Immunology , Cancer , Cancer & Oncology , Cancer Vaccines , Epithelial Cancer , ... We are pioneering approaches to treat advanced CaP that employ conditionally replication-competent oncolytic adenoviruses that ...
Studies of Nondefective Adenovirus 2-Simian Virus 40 Hybrid Viruses. VI. Characterization of the DNA from Five Nondefective...
Studies of Nondefective Adenovirus 2-Simian Virus 40 Hybrid Viruses. VI. Characterization of the DNA from Five Nondefective ... Studies of Nondefective Adenovirus 2-Simian Virus 40 Hybrid Viruses. VI. Characterization of the DNA from Five Nondefective ... Studies of Nondefective Adenovirus 2-Simian Virus 40 Hybrid Viruses. VI. Characterization of the DNA from Five Nondefective ... Studies of Nondefective Adenovirus 2-Simian Virus 40 Hybrid Viruses. VI. Characterization of the DNA from Five Nondefective ...
Simian virus 40-specific proteins in HeLa cells infected with nondefective adenovirus 2-simian virus 40 hybrid viruses. |...
Simian virus 40-specific proteins in HeLa cells infected with nondefective adenovirus 2-simian virus 40 hybrid viruses.. G ... Simian virus 40-specific proteins in HeLa cells infected with nondefective adenovirus 2-simian virus 40 hybrid viruses. ... Simian virus 40-specific proteins in HeLa cells infected with nondefective adenovirus 2-simian virus 40 hybrid viruses. ... Simian virus 40-specific proteins in HeLa cells infected with nondefective adenovirus 2-simian virus 40 hybrid viruses. ...
Development of a novel simian adenovirus 24 based vaccine vector | Retrovirology | Full Text
Human adenovirus serotype 5 is a potent vaccine vector, but its use has been hampered by high seroprevalence amongst people in ... Here we describe the development of a simian Ad24 (sAd24)-based vaccine vector. ... using a panel of 106 rhesus macaque sera and 128 human sera from Rwanda and South Africa using a luciferase-based adenovirus ...
HIV vaccine; Adenovirus serotype 5; Simian Immunodeficiency Virus; CD8+T cells; CD4+T cells; Step trial Archives | BioLife...
Adenovirus E1A, simian virus 40 tumor antigen, and human papillomavirus E7 protein share the capacity to disrupt the...
We suggest that the ability of E1A, the simian virus 40 large tumor antigen, and E7 to dissociate the E2F-pRb complex may be a ... We now show that the E7 protein and the simian virus 40 large tumor antigen can dissociate the E2F-pRb complex, dependent on ... the simian virus 40 large tumor antigen, and the human papillomavirus E7 protein share a short amino acid sequence that ... The adenovirus E1A gene product, the simian virus 40 large tumor antigen, and the human papillomavirus E7 protein share a short ...
EP 1944043 A1 - Simian Adenovirus Nucleic Acid And Amino Acid Sequences, Vectors Containing Same, And Methods Of Use - The...
Protective Efficacy of a Novel Simian Adenovirus Vaccine Against Lethal MERS-CoV Challenge in a Transgenic Human DPP4 Mouse...
Protective Efficacy of a Novel Simian Adenovirus Vaccine Against Lethal MERS-CoV Challenge in a Transgenic Human DPP4 Mouse ... Protective Efficacy of a Novel Simian Adenovirus Vaccine Against Lethal MERS-CoV Challenge in a Transgenic Human DPP4 Mouse ... Protective Efficacy of a Novel Simian Adenovirus Vaccine Against Lethal MERS-CoV Challenge in a Transgenic Human DPP4 Mouse ... A single dose of an adenovirus-vectored vaccine provides complete protection of the upper and lower respiratory tracts against ...
Clinical assessment of a novel recombinant simian adenovirus ChAdOx1 as a vectored vaccine expressing conserved influenza a...
Simian adenoviruses do not suffer from the same drawbacks. We have constructed a replication-deficient chimpanzee adenovirus- ... we report safety and T-cell immunogenicity following vaccination with this novel recombinant simian adenovirus, ChAdOx1 NP+M1, ... However, the widespread seroprevalence of neutralizing antibodies to common human adenovirus serotypes limits their use. ... Adenoviruses are potent vectors for inducing and boosting cellular immunity to encoded recombinant antigens. ...
ArboCat Virus: Simian hemorrhagic fever (SHFV)
Adenoviruses: SV-11, SV-15, SV-17, SV-20, SV-25, SV-27, SV-36, ICH. ... H. simplex, canine herpes, simian cytomegalic, B virus Myxoviruses: Parainfluenza 1, 2, 3, SV-5; avian leukosis (RSV/RIF and ... Positive results were obtained only with sera from simian hemorrhagic fever outbreaks: Sukhumi/64, Bethesda/64, Davis/67, and ...
Simian Virology | Laboratory Animal Medicine | Veterinary Medicine | Subjects | Wiley
21 Adenoviruses.. Section 3: Miscellaneous RNA and DNA Viruses.. 22 Miscellaneous Viruses. ... Simian Virology is the first text to comprehensively cover all currently known simian viruses. Chapters provide an overview of ... "Simian Virology is a comprehensive text covering the biology of viruses whose natural hosts are simian species…The authors of ... Simian Virology Visit authors Marx and Voevodins website for more information and resources on Simian Virology. ...
Simian virus 40 replication in adenovirus-transformed human cells antagonizes gene expression - Dimensions
Vaccine Vectors Derived from a Large Collection of Simian Adenoviruses Induce Potent Cellular Immunity Across Multiple Species ...
Home » Vaccine Vectors Derived from a Large Collection of Simian Adenoviruses Induce Potent Cellular Immunity Across Multiple ... Vaccine Vectors Derived from a Large Collection of Simian Adenoviruses Induce Potent Cellular Immunity Across Multiple Species ... Vaccine Vectors Derived from a Large Collection of Simian Adenoviruses Induce Potent Cellular Immunity Across Multiple Species ... Vaccine Vectors Derived from a Large Collection of Simian Adenoviruses Induce Potent Cellular Immunity Across Multiple Species ...
Isolation of a novel monkey adenovirus reveals a new phylogenetic clade in the evolutionary history of simian adenoviruses |...
Human Adenovirus A to G, and Simian Adenovirus A). In this study, a novel adenovirus was isolated from a colony of cynomolgus ... Sequence analysis of these four genes showed that the new isolate had 80% identity to other primate adenoviruses and lacked ... Adenoviruses of primates include human (HAdV) and simian (SAdV) isolates classified into 8 species ( ... on SAdV and represents an important contribution to the understanding of the evolutionary history of primate adenoviruses. ...
The structure and expression of two defective adenovirus 2/simian virus 40 hybrids - CSHL Scientific Digital Repository
Two new defective adenovirus 2/simian virus 40 hybrids (Ad2+ D1 and Ad2 + D2) have been isolated from the population known as ... The DNA of Ad2+D1 is almost equal in size to that of its helper, adenovirus 2. It contains an insertion of simian virus 40 ( ... The structure and expression of two defective adenovirus 2/simian virus 40 hybrids ... The structure and expression of two defective adenovirus 2/simian virus 40 hybrids. J Mol Biol, 120 (2). pp. 209-47. ISSN 0022- ...
adenovirus | Laboratori de Virus Contaminants d'Aigua i Aliments
Isolation of a novel monkey adenovirus reveals a new phylogenetic clade in the evolutionary history of simian adenoviruses. ... PCR water contamination adenoviruses JC shellfish polyomaviruses fecal contamination JCV adenovirus hepatitis E HEV sewage More ... Read more about Isolation of a novel monkey adenovirus reveals a new phylogenetic clade in the evolutionary history of simian ... Viral pollution in the environment and in shellfish: human adenovirus detection by PCR as an index of human viruses. Language ...
Ask.com - What's Your Question?
Replicating Adenovirus-Simian Immunodeficiency ... - ResearchGate www.researchgate.net/profile/Katherine_Mckinnon/publication/ ... Adenovirus-Simian_Immunodeficiency_Virus_SIV_Vectors_Efficiently_Prime_SIV-Specific_Systemic_and_Mucosal_Immune_Responses_by_ ... Targeting_Myeloid_Dendritic_Cells_and_Persisting_in_Rectal_Macr/links/53d90a1d0cf2e38c6331dfad/Replicating-Adenovirus-Simian- ...
Vero-SF-ACF ATCC ® CCL-81.5™ Cercopithecus aethiops
... and simian adenoviruses.. The parental Vero cell line has been show to be resistant to infection by the following viruses: ... Temperature dependence of the synthesis of adenovirus tumor and viral antigens. Proc. Soc. Exp. Biol. Med. 127: 642-646, 1968. ... Temperature dependence of the synthesis of adenovirus tumor and viral antigens. Proc. Soc. Exp. Biol. Med. 127: 642-646, 1968. ...
HIV-1-neutralizing antibody induced by simian adenovirus- and poxvirus MVA-vectored BG505 native-like envelope trimers. -...
... titres when BG505s trimers were also delivered by non-replicating simian (chimpanzee) adenovirus and non-replicating poxvirus ... HIV-1-neutralizing antibody induced by simian adenovirus- and poxvirus MVA-vectored BG505 native-like envelope trimers. ... AIDS Vaccines, Adenoviruses, Simian, Animals, Antibodies, Neutralizing, Cell Line, HEK293 Cells, HIV Antibodies, HIV-1, Humans ... HIV-1-neutralizing antibody induced by simian adenovirus- and poxvirus MVA-vectored BG505 native-like envelope trimers. ...
Persistent infection with adenovirus types 5 and 6 in lymphoid cells from humans and woolly monkeys
Adenovirus could be recovered from cultures of pri … ... Chronic infection with adenovirus types 5 and 6 was established ... Adenoviruses, Human / pathogenicity * Adenoviruses, Simian / growth & development * Adenoviruses, Simian / pathogenicity * ... Persistent infection with adenovirus types 5 and 6 in lymphoid cells from humans and woolly monkeys J Infect Dis. 1982 Jan;145( ... Adenovirus could be recovered from cultures of primary leukocytes and of EBV-transformed lymphocytes for two and three months, ...
Erratum: Factors associated with slow disease progression in macaques immunized with an adenovirus-simian immunodeficiency...
T2 - Factors associated with slow disease progression in macaques immunized with an adenovirus-simian immunodeficiency virus ( ... Erratum: Factors associated with slow disease progression in macaques immunized with an adenovirus-simian immunodeficiency ... Erratum: Factors associated with slow disease progression in macaques immunized with an adenovirus-simian immunodeficiency ... Erratum : Factors associated with slow disease progression in macaques immunized with an adenovirus-simian immunodeficiency ...
Identification of New Provisional Simian Adenovirus Species from Captive Monkeys, China - Volume 20, Number 10-October 2014 -...
Comparison of the hexon gene sequences of simian adenovirus types identified in this study with human and simian adenovirus ... at least 25 simian adenovirus types have been identified (3). Since 2011, several novel adenoviruses have been isolated from ... Hexon genes of the 10 simian adenovirus strains ranged in length from 2,739 to 2,820 nt and from 913 to 940 aa. Four strains ( ... Identification of New Provisional Simian Adenovirus Species from Captive Monkeys, China On This Page ...
Corrigendum to "Heterologous Two-dose Vaccination with Simian Adenovirus and Poxvirus Vectors Elicits Long-lasting Cellular...
Corrigendum to "Heterologous Two-dose Vaccination with Simian Adenovirus and Poxvirus Vectors Elicits Long-lasting Cellular ... Corrigendum to "Heterologous Two-dose Vaccination with Simian Adenovirus and Poxvirus Vectors Elicits Long-lasting Cellular ... Corrigendum to "Heterologous Two-dose Vaccination with Simian Adenovirus and Poxvirus Vectors Elicits Long-lasting Cellular ...
Ribosomal proteins in normal simian cells, sv40-transformed simian cells, and simian cells infected with sv40, adenovirus 5,...
T1 - Ribosomal proteins in normal simian cells, sv40-transformed simian cells, and simian cells infected with sv40, adenovirus ... Ribosomal proteins in normal simian cells, sv40-transformed simian cells, and simian cells infected with sv40, adenovirus 5, ... Ribosomal proteins in normal simian cells, sv40-transformed simian cells, and simian cells infected with sv40, adenovirus 5, ... Ribosomal proteins in normal simian cells, sv40-transformed simian cells, and simian cells infected with sv40, adenovirus 5, ...
A pan-genotype HCV T cell vaccine, in a simian adenovirus vector, to target T cell epitopes conserved across multiple HCV...
A pan-genotype HCV T cell vaccine, in a simian adenovirus vector, to target T cell epitopes conserved across multiple HCV ... A pan-genotype HCV T cell vaccine, in a simian adenovirus vector, to target T cell epitopes conserved across multiple HCV ... A pan-genotype HCV T cell vaccine, in a simian adenovirus vector, to target T cell epitopes conserved across multiple HCV ...
Simian virus 40 large-T antigen expresses a biological activity complementary to the p300-associated transforming function of...
Adenovirus Early Proteins Animals Antigens, Polyomavirus Transforming/genetics/*metabolism Cell Line Cell Transformation, Viral ... Yaciuk, P., Carter, M. C., Pipas, J. M., Moran, E. (April 1991) Simian virus 40 large-T antigen expresses a biological activity ... Simian virus 40 large-T antigen expresses a biological activity complementary to the p300-associated transforming function of ... In this report we present evidence that simian virus 40 T antigen encodes a biological activity that is functionally equivalent ...
Single-dose protection against Plasmodium berghei by a simian adenovirus vector using a human cytomegalovirus promoter...
We compared the immunogenicity and efficacy of AdC6, a recombinant simian adenovirus serotype 6 vector, in a murine malaria ... Neutralizing antibodies to AdH5 limit the efficacy of vaccines based on the AdH5 serotype, and simian adenoviral vectors offer ... We constructed E1E3-deleted human and simian adenoviral vectors encoding the pre-erythrocytic-stage malarial antigen Plasmodium ... Human adenovirus serotype 5 (AdH5) vector vaccines elicit strong immune responses to the encoded antigen and have been used in ...
SV40VectorsSerotypeImmunodeficiencyVaccinesAntibodiesVectorAntigenSerotypesLeast 25 simian adenovirusViralAdenoviralSequencesGenesSpeciesProteinsVirus 40-specificHumansVirusesInfectionReplicationProteinAntigensInfectionsGeneVirologyDetection of adenovirusesHAdVTranscriptionMacaque adenovirusSAdVStrainsInduceTumorsVaccinationTypeHerpesvirusesPrimatesIsolatesAcuteResponsesMurine
- Certain biophysical characteristics of the DNA from each of the five nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid viruses (Ad2 + ND 1 , Ad2 + ND 2 , Ad2 + ND 3 , Ad2 + ND 4 , Ad2 + ND 5 ) have been determined. (asm.org)
- The synthesis of simian virus 40 (SV40)-specific proteins in HeLa cells infected with the nondefective adenovirus 2 (Ad2)-SV40 hybrid viruses, Ad2+ND2, Ad2+ND3, Ad2+ND4, and Ad2+ND5, was investigated. (asm.org)
- It contains an insertion of simian virus 40 (SV40) sequences 3.2 × 103 bases long in place of the 3.5 × 103 base segment of adenovirus 2 DNA which maps between 0.64 and 0.74 fractional genome lengths from the left end of the viral DNA. (cshl.edu)
- The segment mapping between 0.76 and 0.96 fractional genome lengths from the left end of adenovirus 2 DNA is absent: in its place is inserted a stretch of DNA, 5.0 × 103 bases in length which comprises all the sequences of the SV40 genome apart from those lying between map positions 54 and 63. (cshl.edu)
- Consideration of the structures of the genomes of Ad2+D1 and Ad2+D2 leads us to believe that Ad2+D1 may have arisen by recombination between members of the Ad2++HEY hybrid pool and adenovirus 2, while Ad2+D2 was most likely generated by recombination events between adenovirus 2 and free SV40. (cshl.edu)
- The protein patterns of monosomes and polysomes isolated from the T-22 line of SV40-transformed GMK cells and from uninfected CV-I cells and CV-1 cells infected with SV40, adenovirus 5, or vesicular stomatitis virus were analyzed by two-dimensional PAGE. (okstate.edu)
- Polyclonal antibodies were used to assay human embryonal carcinoma (EC), differentiating EC, yolk sac carcinoma, and teratoma cells for expression of viral early antigen (T-Ag) after infection with simian virus 40 (SV40). (uthscsa.edu)
- Also, viral late proteins were produced by EC cells infected with human adenovirus type 7 (Ad7), and SV40 T-Ag was expressed by EC cells after infection with PARA, which is an Ad7-SV40 hybrid virus containing the SV40 T-Ag sequence controlled by Ad7 late regulatory sequences. (uthscsa.edu)
- When SV80 cells were infected with type C adenovirus, both the synthesis of SV40 large T-antigen and the appearance in the cytoplasm of newly synthesized, SV40-specific RNA sequences were inhibited during the late phase of infection. (princeton.edu)
- The results of hybridization to SV40 DNA of SV80 nuclear RNA, prepared from mock- or adenovirus-infected cells after labeling for short periods in vivo or in vitro, indicated that transcription of integrated SV40 was, by contrast, not disrupted during the late phase of adenovirus infection. (princeton.edu)
- Several lines of evidence do, however, establish that nuclear, SV40-specific RNA sequences are less stable in adenovirus-infected compared to mock-infected SV80 cells. (princeton.edu)
- Simian virus 40 (SV40) was an accidental contaminant of vaccines produced in monkey kidney tissue cultures in the 1950s and early 1960s, including a parenteral adenovirus vaccine given to several hundred thousand US military recruits. (oup.com)
- To determine if entry into US Army service during periods of administration of SV40-contaminated adenovirus vaccine was associated with an increased risk of cancer, the authors conducted a case-control study of cancer occurring in male Army. (oup.com)
- The odds ratios associated with exposure to SV40-contaminated adenovirus vaccine were 0.81 (95% confidence interval (CI): 0.52, 1.24) for brain tumors, 1.41 (95% CI: 0.39, 5.15) for mesothelioma, and 0.97 (95% CI: 0.65, 1.44) for non-Hodgkin's lymphoma. (oup.com)
- SV40, simian virus 40. (oup.com)
- This virus was named Simian vacuolating virus (SV40). (hindawi.com)
- Some of the polio vaccine administered from 1955-1963 was unknowingly contaminated with a virus, called simian virus 40 (SV40). (immunizationinfo.org)
- O SV40 , do inglés simian vacuolating virus 40 ou simian virus 40 (virus simio vacuolante 40 ou virus simio 40), é un poliomavirus que se encontra en monos e humanos . (wikipedia.org)
- Como outros poliomavirus, o SV40 é un virus de ADN que ten o potencial de causar tumores en animais, pero máis frecuentemente persiste como infección latente. (wikipedia.org)
- O descubrimento do SV40 en monos revelou que entre 1955 e 1963 moitas persoas foron inoculadas inadvertidamente co virus SV40, xa que este contaminaba as vacinas da polio , que se facían utilizando células de monos (por exemplo, nos Estados Unidos o 90% dos nenos e o 60% dos adultos foron inoculados con estas vacinas ), aínda que non se observou ningún efecto nocivo. (wikipedia.org)
- A hipótese de que o SV40 podería causar cancro en humanos foi unha área de investigación especialmente controvertida. (wikipedia.org)
- [ 6 ] Actualmente non está claro que o SV40 teña algún papel en causar tumores. (wikipedia.org)
- [ 15 ] [ 16 ] O mecanismo pode implicar a supresión das propiedades transcricionais do supresor de tumores p53 en humanos polo antíxenos T grande e pequeno de SV40. (wikipedia.org)
- The fibroblast line was originally utilized in research focusing on the transformation of the cancer-causing Rous sarcoma virus ( RSV ), but now is popular as a host for acquired immunodeficiency disease ( AIDS ) research, as well as transfection experiments with simian virus 40 ( SV40 ) and recombinant plasmid vectors. (microscopyu.com)
- When it comes to vaccine vectors, the pool of human adenovirus candidates has been quite shallow. (nouscom.com)
- As with human adenoviral vectors, different simian vectors induced either more or less potent immune responses in mice. (nouscom.com)
- Here, we assessed the induction of HIV-1 trimer binding and neutralizing antibody (nAb) titres when BG505s trimers were also delivered by non-replicating simian (chimpanzee) adenovirus and non-replicating poxvirus modified vaccinia virus Ankara (MVA) vaccine vectors. (ox.ac.uk)
- Neutralizing antibodies to AdH5 limit the efficacy of vaccines based on the AdH5 serotype, and simian adenoviral vectors offer an attractive option to overcome this problem. (ox.ac.uk)
- We constructed E1E3-deleted human and simian adenoviral vectors encoding the pre-erythrocytic-stage malarial antigen Plasmodium berghei circumsporozoite protein. (ox.ac.uk)
- We compared the immunogenicity and efficacy of AdC6, a recombinant simian adenovirus serotype 6 vector, in a murine malaria model to those of AdH5 and the poxviral vectors MVA and FP9. (ox.ac.uk)
- Adenoviruses are effective vectors for inducing and boosting cellular immunity to encoded recombinant antigens. (wikipedia.org)
- Described are vaccines comprising recombinant vectors, such as recombinant adenoviruses. (patents.com)
- In the present study, we tested by the hypothesis that the Th1 immune response to CS protein, in particular the CD8 + T-cell response, which is needed for strong and lasting malaria immunity, is boosted to sustainable levels vectors adenovirus and 26 with an homologous insert 35 (Ad35.CS/Ad26.CS). (asm.org)
- To explore differential regulation of Gag and Env antibody responses, immunocompetent BALB/c and T cell deficient nude mice were immunized with virus like particles (VLP) of simian immunodeficiency virus or adenoviral vectors expressing SIV Gag and Env. (biomedcentral.com)
- We also generated simian and human recombinant replication-defective adenovirus vectors expressing PvCSP-All-CSP-epitopes. (jove.com)
- So far, simian virus 40, bovine papillomavirus, adenovirus and members of the retrovirus family have been used the most extensively as expression vectors. (fao.org)
- Vaccines based on simian adenoviral vectors have been extensively evaluated in Phase 1 and 2 clinical trials and proved to be safe and immunogenic. (prnewswire.co.uk)
- Adenoviruses have been studied intensively for over 50 years as models of virus-cell interactions and latterly as gene vectors. (psu.edu)
- Human adenovirus serotype 5 is a potent vaccine vector, but its use has been hampered by high seroprevalence amongst people in sub-Sahara Africa. (biomedcentral.com)
- Human adenovirus serotype 5 (AdH5) vector vaccines elicit strong immune responses to the encoded antigen and have been used in various disease models. (ox.ac.uk)
- ChAdOx1 has been derived from a simian adenovirus (ChAd) serotype Y25 engineered by λ red recombination to exchange the native E4 orf4, orf6 and orf6/7 genes for those from human adenovirus HAdV-C5. (wikipedia.org)
- We assessed neutralizing antibody titers to adenovirus serotype 5 (Ad5) and six rare adenovirus serotypes, serotypes 11, 35, 50, 26, 48, and 49, in pediatric populations in sub-Saharan Africa. (asm.org)
- Replication-incompetent adenovirus serotype 5 (Ad5) vector-based vaccines for human immunodeficiency virus type 1 (HIV-1) have proven highly immunogenic in preclinical studies and are being advanced into large-scale clinical trials. (asm.org)
- Acute respiratory disease associated with adenovirus serotype 14--four states, 2006-2007. (semanticscholar.org)
- During human (HIV) and simian (SIV) immunodeficiency virus infection, loss of CD4+ T cells and progression to AIDS are associated with a decline in antibody titers to the viral Gag protein, while antibodies to the Env protein remain high, suggesting a T cell independent antibody response to Env. (biomedcentral.com)
- In vivo, simian immunodeficiency virus (SIV)-specific CD8 + and CD4 + T-cell responses were induced in monkeys using SIV antigen-expressing DCs. (bloodjournal.org)
- The various vaccine combinations were tested by blending monovalent recombinant adenovirus vaccines, each expressing hemagglutinin from a single strain. (springer.com)
- Therefore, investigators have tested new vaccines using the chimp adenovirus ChAdOx1 as a vector. (wikipedia.org)
- Our expertise and positive preclinical and clinical data on adenovirus-based vaccines, allows us to react to this unprecedented situation in a prompt and timely framework. (prnewswire.co.uk)
- Furthermore, our results highlight the potential of using these non-human primates as models for testing safety and efficacy of candidate adenovirus vaccines prior to clinical trials in humans. (biomedsearch.com)
- Live oral adenovirus vaccines have properties that are well-suited for use in such settings. (asm.org)
- The induction of a strong cellular response by the recombinant indicates that live adenovirus recombinants have potential as vaccines for those agents. (asm.org)
- These studies encourage and will inform the continued development of viable recombinant adenovirus vaccines. (asm.org)
- Live adenovirus vaccines have been used by the United States military for decades to prevent adenovirus type 4 (Ad4)- and Ad7-induced severe upper respiratory disease in recruits ( 1 ). (asm.org)
- Neutralizing antibodies against sAd24 were determined using a panel of 106 rhesus macaque sera and 128 human sera from Rwanda and South Africa using a luciferase-based adenovirus neutralization assay. (biomedcentral.com)
- However, the widespread seroprevalence of neutralizing antibodies to common human adenovirus serotypes limits their use. (wikipedia.org)
- Antibodies to Synthetic Peptides Targeted to the Transforming Genes of Human Adenoviruses - An Approach to Understanding Early Viral Gene Function. (wisepress.com)
- Re-examining the samples many years later, Chiu and his colleagues found antibodies targeted to simian adenovirus C in the human samples. (healthcanal.com)
- Here we describe the development of a simian Ad24 (sAd24)-based vaccine vector. (biomedcentral.com)
- Therefore, the authors deepened the vector pool by isolating more than 1000 adenovirus strains from chimpanzees. (nouscom.com)
- Single-dose protection against Plasmodium berghei by a simian adenovirus vector using a human cytomegalovirus promoter containing intron A. (ox.ac.uk)
- The vector is a chimpanzee adenovirus modified to avoid its replication. (wikipedia.org)
- The adenovirus expressing the antigen 85A (ChAdOx1 85A), is used as vector for a tuberculosis vaccine candidate. (wikipedia.org)
- 9. The recombinant polynucleotide vector of claim 8, wherein the recombinant polynucleotide vector is packaged into a replication-defective adenovirus selected from the group consisting of human adenovirus serotypes Ad11, Ad24, Ad26, Ad34, Ad35, Ad48, Ad49, and Ad50. (patents.com)
- The ChAdOx1 MERS vaccine consists of the replication-deficient simian adenovirus vector ChAdOx1, containing the MERS Spike protein antigen. (clinicaltrials.gov)
- The vaccine technology is based on a novel, ReiThera-proprietary simian adenoviral vector with strong immunological potency and low pre-existing immunity in humans. (prnewswire.co.uk)
- The simian adenovirus, ChAdOx1, and modified vaccinia Ankara virus, MVA, encoding a prostate cancer-associated antigen, the six transmembrane epithelial antigen of the prostate 1 (STEAP1), induced strong sustained antigen-specific CD8+ T-cell responses in C57BL/6 and BALB/c male mice. (medworm.com)
- Adenovirus E1A, simian virus 40 tumor antigen, and human papillomavirus E7 protein share the capacity to disrupt the interaction between transcription factor E2F and the retinoblastoma gene product. (semanticscholar.org)
- The adenovirus E1A gene product, the simian virus 40 large tumor antigen, and the human papillomavirus E7 protein share a short amino acid sequence that constitutes a domain required for the transforming activity of these proteins. (semanticscholar.org)
- In this report we present evidence that simian virus 40 T antigen encodes a biological activity that is functionally equivalent to the transforming activity lost by deletion of the E1A p300-binding region. (cshl.edu)
- This is an open label phase I study, to assess the safety of a novel malaria vaccine, AdCh63 AMA1, simian adenovirus encoding Plasmodium falciparum blood stage antigen, Apical Membrane Antigen -1. (clinicaltrials.gov)
- The B subunit also is replaceable by viral proteins, such as the simian virus 40 small t antigen and the polyomavirus small and middle T antigens ( 13 ). (pnas.org)
- All the faecal samples were screened for presence of adenoviruses using a commercial antigen-capture enzyme immunoassay (EIA) kit, this is an enzyme-linked immunosorbent assay (ELISA) kit designed for diagnosis of human enteric adenoviruses in stool samples. (biomedsearch.com)
Least 25 simian adenovirus1
- In addition, at least 25 simian adenovirus types have been identified ( 3 ). (cdc.gov)
- The text covers a variety of topics including primate models of medically important viral diseases such as AIDS, hypotheses on the origins of epidemic forms of HIV, and viral diseases caused by non-simian viruses in both wild and captive primates. (wiley.com)
- Part II: Simian Viruses and Nonhuman Primate Models of Viral Infections. (wiley.com)
- Temperature dependence of the synthesis of adenovirus tumor and viral antigens. (atcc.org)
- The exciting discoveries made with adenoviruses have had such a pro- found effect on knowledge in basic virology, molecular biology, viral ge- netics, human and animal infections, and cell transformation that this seemed a propitious time to have some of the major contributors review this field. (springer.com)
- The VA (viral associated) RNA is a type of non-coding RNA found in adenovirus. (wikipedia.org)
- These results suggest that the failure of non-adenoviral mRNA sequences to enter the cytoplasm of adenovirus-infected cells does not reflect inhibition of either their transcription or the normal enzymatic processing reactions to which pre-mRNA species are subject. (princeton.edu)
- The induced AP-1 activates transcription of early adenovirus genes through AP-1 and activating transcription factor sites in adenoviral promoters ( 1 ). (pnas.org)
- [ 25 ] In one study involving children who underwent hematopoietic stem cell transplantation, all patients who died of adenoviral infection lacked specific T cells against adenovirus. (medscape.com)
- We performed alignment analysis of the complete nucleotide hexon sequences of the 10 novel strains and of reference human and simian adenoviruses by using the MUSCLE program within the MEGA5 software package ( 6 ). (cdc.gov)
- Phylogenetic analysis of complete hexon gene nucleotide sequences from human and simian adenoviruses. (cdc.gov)
- Comparison of the hexon gene sequences of simian adenovirus types identified in this study with human and simian adenovirus sequences from GenBank revealed 9 previously reported hypervariable regions (HVRs): HVR1-HVR9 ( 5 , 7 ). (cdc.gov)
- Sequence analysis of these four genes showed that the new isolate had 80% identity to other primate adenoviruses and lacked recombination events. (biomedcentral.com)
- Moreover, the hexon and the protease genes have been characterized in most adenovirus types providing a large amount of data for taxonomic classification of new isolates. (biomedcentral.com)
- Hexon genes of the 10 simian adenovirus strains ranged in length from 2,739 to 2,820 nt and from 913 to 940 aa. (cdc.gov)
- Previous work has shown that the adenovirus E1A proteins and cAMP cooperate to induce the accumulation of activator protein 1 (AP-1) transcription factor by activating transcription of the cellular c- fos and junB genes that encode the AP-1 components. (pnas.org)
- In Vitro Transcription of Adenovirus Genes. (wisepress.com)
- These two VA RNA genes are distinct genes in the adenovirus genome. (wikipedia.org)
- Simian Virology is a comprehensive text covering the biology of viruses whose natural hosts are simian species…The authors of the text are each noted researchers in the field of simian retrovirology. (wiley.com)
- One simian adenovirus species, SAdV-A, has been recognized by the International Committee on Taxonomy of Viruses ( 2 ). (cdc.gov)
- For more accurate characterization and for possible species determination of adenovirus strains, it is beneficial to sequence the entire hexon gene. (cdc.gov)
- Thus, we propose a new candidate simian species, named SAdV-D, for this new cluster of macaque adenovirus strains. (cdc.gov)
- Our phylogenetic analysis also identified another possible new simian species: 5 strains, which were previously reported as "unassigned adenoviruses," were isolated from Old World monkeys living in captivity and in the wild ( 8 ). (cdc.gov)
- Asterisks (*) indicate the proposed new candidate simian species. (cdc.gov)
- Adenoviruses commonly infect humans, causing colds, flu-like symptoms and sometimes even death, but now UC San Francisco researchers have discovered that a new species of adenovirus can spread from primate to primate, and potentially from monkey to human. (healthcanal.com)
- Adenoviruses to date have not generally been linked to cross-species infections between monkeys and humans," Chiu said. (healthcanal.com)
- In contrast, viruses that use DNA as their genetic material, such as adenoviruses, are thought to have less chance of spreading between species because they replicate with fewer mutations that could serve as the basis for infection-enhancing changes. (healthcanal.com)
- Adenovirus keratitis was induced in C57Bl/6j mice by intrastromal injection of human adenovirus species D type 37 (HAdV-D37) with a heat-pulled, glass, micropipette needle under compressed air. (arvojournals.org)
- These findings suggest that cross species transmission in Kenyan non-human primates may be a common occurrence and there is a possibility of zoonotic transmission of adenoviruses. (biomedsearch.com)
- Prologue Adenoviruses (Ads) are relatively promiscuous in their ability to infect a wide range of species and tissues, but their propensity to produce disease is normally well circumscribed and is a function of the effective defences that are mounted by the infected host. (psu.edu)
- Simian virus 40-specific proteins in HeLa cells infected with nondefective adenovirus 2-simian virus 40 hybrid viruses. (asm.org)
- The Mechanism of Adenovirus DNA Replication and the Characterization of Replication Proteins. (wisepress.com)
- The immunization of macaques induced vigorous humoral responses to adenovirus capsid and nonstructural proteins, although, surprisingly, not against HPV L1. (asm.org)
- Linzer, D. I.H. / Synthesis and processing of simian virus 40-specific RNA in adenovirus-infected, simian virus 40-transformed human cells . (princeton.edu)
- Adenoviruses commonly infect vertebrates, including humans and nonhuman primates ( 1 ). (cdc.gov)
- Simian adenoviruses have not been used previously in a clinical trial in humans. (clinicaltrials.gov)
- In light of these findings, however, he said the normal vigilance in tracking animal viruses that might also infect humans should extend beyond influenza and coronaviruses to include adenoviruses. (healthcanal.com)
- Studies of Nondefective Adenovirus 2-Simian Virus 40 Hybrid Viruses. (asm.org)
- Simian Virology is the first text to comprehensively cover all currently known simian viruses. (wiley.com)
- Section 1.2: Other Simian RNA Viruses. (wiley.com)
- Section 2.2: Other Simian DNA Viruses. (wiley.com)
- Simian Virology is a well-organized and comprehensive book that should be useful for undergraduate and graduate students, veterinarians, biologists, and other professionals who work with nonhuman primates (NHPs), viruses or NHPs, or NHPs involved in research on human disease. (wiley.com)
- The genomes of the two nondefective adenovirus 2/simian virus 40 (Ad2/ SV 40) hybrid viruses, nondefective Ad2/SV 40 hybrid virus 1 (Ad2 + ND 1 ) and nondefective hybrid virus 3 (Ad2 + ND 3 ), were formed by a deletion of about 5% of Ad2 DNA and insertion of part of the SV 40 genome. (princeton.edu)
- The discovery of adenoviruses naturally induced a new interest in viruses of the human upper respiratory tract since previously unknown viruses infecting this portion of the human body had not been identified in 20 years, and their unique characteristics stimulated investigations into the biochemical events essential for replication of animal viruses. (springer.com)
- In 1962, some of the adenoviruses were the first human viruses shown to be oncogenic in experimental animals. (wisepress.com)
- Chronic infection with adenovirus types 5 and 6 was established in primary mononuclear leukocytes from human umbilical cord blood and in Epstein-Barr virus (EBV)-transformed B lymphocytes from human umbilical cord blood and from woolly monkey blood. (nih.gov)
- Thus, chronic infection of lymphoid cells by some adenoviruses is maintained by at least two mechanisms: cell-to-cell spread of virus in the absence of antibody and intracellular persistence of infectious virus in the presence of antibody. (nih.gov)
- Currently, specific therapy for adenovirus infection, other than supportive and symptomatic treatment, remains a matter of debate. (medscape.com)
- Large epidemic of adenovirus type 4 infection among military trainees: epidemiological, clinical, and laboratory studies. (semanticscholar.org)
- Rapid type-specific diagnosis of adenovirus type 4 infection using a hexon-based quantitative fluorogenic PCR. (semanticscholar.org)
- The HAdVs do not cause productive infection in mice, nor are mouse adenoviruses known to cause murine keratitis in the wild. (arvojournals.org)
- These diseases associated with adenovirus infection affect adults and are usually more severe in infants and children. (biomedsearch.com)
- To date, limited studies have been done to determine the prevalence of adenovirus infection in non-human primates with the goal of developing a non-human primate model that can be used to study the mechanisms of infection. (biomedsearch.com)
- OBJECTIVE: To determine the prevalence of enteric adenovirus infection in Kenyan non-human primates. (biomedsearch.com)
- 0.05) in adenovirus infection signifying equal susceptibility. (biomedsearch.com)
- CONCLUSION: The results of this study indicate that adenovirus infection is prevalent among non-human primates in Kenya. (biomedsearch.com)
- In addition to the γ determinant of the fiber protein, the main type-specific epitope in adenovirus is the ε determinant present on the hexon capsid protein. (biomedcentral.com)
- Hexon is the most abundant protein in the icosahedral capsid, and the hexon gene is commonly analyzed to characterize and determine adenovirus types ( 5 ). (cdc.gov)
- We previously have shown that adenovirus type 5 E4orf4 protein associates with protein phosphatase 2A (PP2A) and induces apoptosis in transformed cells in a p53-independent manner. (pnas.org)
- The adenovirus type 5 E4orf4 protein has been shown to affect several cellular processes, including down-regulation of virally induced signal transduction, regulation of gene expression, and induction of apoptosis. (pnas.org)
- The induction of AP-1 by E1A plus cAMP is counterbalanced by the 14-kDa adenovirus E4orf4 protein whose levels rise upon stimulation by E1A and cAMP ( 2 ). (pnas.org)
- Adenovirus E1a protein presents an interesting paradox. (aacrjournals.org)
- Antibody titers were similar in mice immunized with homologous (protein-protein) and heterologous (adenovirus-protein) vaccine regimens. (jove.com)
- We have described a live recombinant adenovirus vaccine prototype that produces abundant HPV16 L1 protein from the adenovirus major late transcriptional unit and directs the assembly of HPV16 VLPs in tissue culture. (asm.org)
- There are a number of trials currently ongoing in Oxford which are aimed at examining a simian adenovirus as a delivery vehicle and liver and blood stage malaria antigens as inserts. (clinicaltrials.gov)
- Epidemiology of adenovirus respiratory infections in military recruit populations. (semanticscholar.org)
- Adenovirus Early Gene Regulation and the Adeno-associated Virus Helper Effect. (wisepress.com)
- Simian Virology Visit authors Marx and Voevodin's website for more information and resources on Simian Virology. (wiley.com)
- Indeed, the field of molecular virology has evolved during the period since their dis- covery, and adenoviruses have played a major role in this development. (springer.com)
- This volume pays tribute to the late Wallace Rowe, Robert Huebner, and Maurice Hilleman whose initial discoveries of adenoviruses have tremendously enriched virology. (springer.com)
Detection of adenoviruses1
- Serological detection of adenoviruses in non-human primates maintained in a colony in Kenya. (biomedsearch.com)
- To date, at least 52 distinct human adenovirus (HAdV) types have been described. (biomedcentral.com)
- Human adenovirus (HAdV) infects all human mucosal epithelia, including most notably those of the respiratory, gastrointestinal, genitourinary, and ocular surfaces, and can be associated with significant mortality and morbidity. (arvojournals.org)
- We determined a complete genome sequence of the Korean field strain, KUMC-62, of human adenovirus type 3 (HAdV-3) and performed comparative genome analyses. (bioportfolio.com)
- Genome Sequence of a Cynomolgus Macaque Adenovirus (CynAdV-1) Isolate from a Primate Colony in the United Kingdom. (ub.edu)
- We previously reported the detection of several new simian adenovirus strains in fecal specimens from captive monkeys in China ( 3 ). (cdc.gov)
- Chiu concluded that staff members had been exposed to the new virus, and that the virus may have jumped from baboon to human, an idea also supported by follow-up experiments in which laboratory strains of simian adenovirus C efficiently infected both human and baboon cells. (healthcanal.com)
- In 2009, scientists demonstrated that a more virulent strain of human adenovirus arose from recombination with other distinct strains of milder human adenoviruses. (healthcanal.com)
- Datta S.K., Trentin J.J., McCormick K.J. (1981) Prevention of Primary Simian Adenovirus Type 7 (SA 7 ) Tumors in Hamsters by Adoptive Transfer of Lymphoid Cells: Role of Different Cell Types. (springer.com)
- Poliovirus Vaccination during Pregnancy, Maternal Seroconversion to Simian Virus 40, and Risk of Childhood Cancer. (immunizationinfo.org)
- Secondary and tertiary structure in the central domain of adenovirus type 2 VA RNA I". RNA. (wikipedia.org)
- Safety evaluation of adenovirus type 4 and type 7 vaccine live, oral in military recruits. (semanticscholar.org)
- The possible origin of human adenovirus type 3: Evidence of natural genetic recombination between human and simian adenovirus. (bioportfolio.com)
- Section 2.1: Simian Herpesviruses. (wiley.com)
- Adenoviruses infecting primates belong to the genus Mastadenovirus . (biomedcentral.com)
- BACKGROUND: Adenoviruses are known to cause several human diseases including acute febrile respiratory syndromes, epidemic conjunctivitis and gastroenteritis. (biomedsearch.com)
- In contrast, immunization elicited strong T-cell responses to HPV VLPs as well as adenovirus virions. (asm.org)
- To determine the relationship between IRES-mediated translation and poliovirus tropism, recombinant human adenoviruses were used to express bicistronic mRNAs in murine organs. (jci.org)