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Unformatted text preview: - a virus acts as a vector (carrier) to transfer small pieces of DNA from one bacterium to another c) Bacterial Transformation – transfer of genetic information into a cell by direct uptake of DNA 4. What term refers to a time when bacterial cells are able to take up or accept DNA? competent 5. What type of bacteria will be transformed in this lab? E. Coli 6. How much cold calcium chloride will be added to each tube? 250 microliters 7. What will be used to add about 10ul of plasmid DNA to the tube? micropipette 8. To which tube will plasmid DNA be added? Tube + 9. How long you “heat shock” the tubes and at what temperature? 90 seconds & 42 degrees Celsius 10. Once tube has incubated on ice, what will be added to the tube? 250 microliters of Luria-Bertani broth 11.How many nutrient agar plates will are required for this investigation? 4...
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This note was uploaded on 10/05/2010 for the course BIO 101 taught by Professor Salazar during the Spring '10 term at Punjab Engineering College.
- Spring '10