Simplified Subtraction-Hybridization System for Isolation Of Strain-Specific Rhizobium DNA Probes
Subtraction-hybridization is the name given to procedures for removing nucleic acid sequences from the genome of an organism which are shared with related organisms. Sequences remaining are those which are unique to the organism from which they are derived. Scott et al (1983) used such a technique to isolate particular genes from transformed mouse cells and Welcher et al (1986) extended the concept to bacteria for the isolation of Neisseria gonorrhoeae specific clones. This paper describes the development of subtraction systems for generating Rhizobium DNA sequences capable of discrimination at inter-strain level when used as probes.