- General Information
Key Terms 3 min
What Are Antibodies? 5 min
Learn about antibody structure and the difference between affinity and avidity.
Antibodies in the Western Blot Process 2 min
Look at the Western blot workflow and where antibodies play a crucial role.
- Primary Antibodies
Polyclonal Antibodies 3 min
Learn how polyclonal antibodies are made and how that leads to their advantages and disadvantages.
Monoclonal Antibodies 2 min
Learn how monoclonal antibodies differ from polyclonal antibodies, and how that leads to their advantages and disadvantages.
Recombinant Antibodies 2 min
Learn how recombinant antibodies are made and how that leads to their advantages and disadvantages.
Primary Antibody Comparison 1 min
Compare the different types of primary antibodies to find the one that fits your experiment best.
- Secondary Antibodies
Secondary Antibodies 2 min
Learn about the basic characteristics of a secondary antibody, including clonality and labeling options.
Course Summary 1 min
A summary of the information learned within the course.
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Antibody Ins and Outs
Learn about the importance of antibodies and how their different qualities can impact your experiments.
Antibodies play a large role in Western blotting. This only increases the importance of choosing the correct antibodies for your experiment. This course will explain the various details of both primary and secondary antibodies, giving you a firm foundation for any antibody work in your future research.