Summary: Estrogens were significantly elevated in fetuses that later went on to develop autism. Higher levels of prenatal estrogens were more predictive of ASD than higher levels of prenatal androgens. The study supports a twenty-year-old theory that increased prenatal sex steroid hormones are a potential cause of autism spectrum disorder.
Source: University of Cambridge
Scientist have identified a link between exposure to high levels of estrogen sex hormones in the womb and the likelihood of developing autism. The findings are published today in the journal Molecular Psychiatry.
The discovery adds further evidence to support the prenatal sex steroid theory of autism first proposed 20 years ago.
In 2015, a team of scientists at the University of Cambridge and the State Serum Institute in Denmark measured the levels of four prenatal steroid hormones, including two known as androgens, in the amniotic fluid in the womb and discovered that they were higher in male fetuses who later developed autism. These androgens are produced in higher quantities in male than in female fetuses on average, so might also explain why autism occurs more often in boys. They are also known to masculinize parts of the brain and to have effects on the number of connections between brain cells.
Today, the same scientists have built on their previous findings by testing the amniotic fluid samples from the same 98 individuals sampled from the Danish Biobank, which has collected amniotic samples from over 100,000 pregnancies, but this time looking at another set of prenatal sex steroid hormones called estrogens. This is an important next step because some of the hormones previously studied are directly converted into estrogens.
All four estrogens were significantly elevated, on average, in the 98 fetuses who later developed autism, compared to the 177 fetuses who did not. High levels of prenatal estrogens were even more predictive of the likelihood of autism than were high levels of prenatal androgens (such as testosterone). Contrary to popular belief that associates estrogens with feminization, prenatal estrogens have effects on brain growth and also masculinize the brain in many mammals.
Professor Simon Baron-Cohen, Director of the Autism Research Centre at the University of Cambridge, who led this study and who first proposed the prenatal sex steroid theory of autism, said: “This new finding supports the idea that increased prenatal sex steroid hormones are one of the potential causes for the condition. Genetics is well established as another, and these hormones likely interact with genetic factors to affect the developing fetal brain.”
Alex Tsompanidis, a Ph.D. student in Cambridge who worked on the study, said: “These elevated hormones could be coming from the mother, the baby or the placenta. Our next step should be to study all these possible sources and how they interact during pregnancy.”
Dr. Alexa Pohl, part of the Cambridge team, said: “This finding is exciting because the role of estrogens in autism has hardly been studied, and we hope that we can learn more about how they contribute to fetal brain development in further experiments. We still need to see whether the same result holds true in autistic females.”
However, the team cautioned that these findings cannot and should not be used to screen for autism.
“We are interested in understanding autism, not preventing it,” added Professor Baron-Cohen.
Dr. Arieh Cohen, the biochemist on the team, based at the State Serum Institute in Copenhagen, said: “This is a terrific example of how a unique biobank set up 40 years ago is still reaping scientific fruit today in unimagined ways, through international collaboration.”
Elevated latent prenatal steroidogenic activity has been found in the amniotic fluid of autistic boys, based on measuring prenatal androgens and other steroid hormones. To date, it is unclear if other prenatal steroids also contribute to autism likelihood. Prenatal estrogens need to be investigated, as they play a key role in synaptogenesis and corticogenesis during prenatal development, in both males and females. Here we test whether levels of prenatal oestriol, oestradiol, oestrone and oestrone sulphate in amniotic fluid are associated with autism, in the same Danish Historic Birth Cohort, in which prenatal androgens were measured, using univariate logistic regression (n = 98 cases, n = 177 controls). We also make a like-to-like comparison between the prenatal estrogens and androgens. Oestradiol, oestrone, oestriol and progesterone each related to autism in univariate analyses after correction with false discovery rate. A comparison of standardised odds ratios showed that oestradiol, oestrone and progesterone had the largest effects on autism likelihood. These results for the first time show that prenatal oestrogens contribute to autism likelihood, extending the finding of elevated prenatal steroidogenic activity in autism. This likely affects sexual differentiation, brain development and function.