The genome harbors additional codes. The genetic code was just the first one to be discovered. In order to decipher and decode the genome, we need to make a Biological Rosetta stone. Our research is dedicated to achieving this goal.
To decipher encoding rules, we use an iterative approach, which begins with a model-based design of DNA sequences, synthetic biology experiments, analysis using advanced bioinformatic, thermodynamic, and stochastic models. The results of the experiments and analysis feed-back on the design model, which generates an improved set of sequences until a robust model for the encoding rules is generated.
We design and construct oligo libraries of tens of thousands of synthetic DNA sequences using various numerical approaches.
We use state-of-the-art liquid automated robotics, next generation sequencing and time-lapse imaging to carry out our measurements.
We carry out our experiments in vivo in a host of model cell lines: bacteria, yeast, mammalian cell lines.