You are here: Home
/ Publication Information
Title: Distribution of Xylella fastidiosa in Sycamore associated with low temperature and host resistance
Author: Henneberger, T.S.M.; Stevenson, K.L.; Britton, Kerry O.; Chang, C.J.;
Source: Plant Disease Vol. 88 No. 9, Pp. 951-960
Publication Series: Miscellaneous Publication
Description: Experiments were conducted in the field and laboratory to determine effects of low temperatures 4% on Xylella fastidiosa populations in American sycamore. Roots and shoots from naturally infected trees at two locations were collected monthly. Sap extracted from the samples was tested by enzyme-linked immunosorbent assay for presence of X fastidosa and was diluted and plated on periwinkle wilt medium to determine populations of viable bacteria. Cumulative rainfall and hours below temperature thresholds (-5 to 10°C) were recorded at each site. Bacterial populations in shoots were negatively correlated with cumulative hours below -5°C air temperature (r = -0.96). In roots, bacterial populations were only weakly correlated with cumulative hours below soil temperature thresholds (-0.61 < r- < -0.25). Bacterial populations were not correlated with monthly rainfall. In the laboratory, resistant and susceptible sycamore trees were inoculated with X. fastidiosa and held in the dark at 5°C or 22°C. After 12 weeks, inoculated stem sections were collected and sap was extracted and tested as described previously. Stems that tested positive for X. fnstidiosa were divided into additional samples and tested as described above. Results of the laboratory study indicated no significant effects of low-temperature treatment (5°C) or host resistance on viable bacteria. Bacterial detection frequency and population size were greatest near the inoculation point and the primary direction of early bacterial spread was acropetal.
Keywords: mechanical inoculation, sycamore decline
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
- You may send email to email@example.com to request a hard copy of this publication. (Please specify exactly
which publication you are requesting and your mailing address.)
XML: View XML
Henneberger, T.S.M.; Stevenson, K.L.; Britton, Kerry O.; Chang, C.J. 2004. Distribution of Xylella fastidiosa in Sycamore associated with low temperature and host resistance. Plant Disease Vol. 88 No. 9, Pp. 951-960
Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility