Suction Pipette Technique: An Electrophysiological Tool to Study Olfactory Receptor-Dependent Signal Transduction
The first step to perceive molecules in the air as odors is their detection by the olfactory receptors (ORs) present in the cilia of the olfactory sensory neurons (OSNs) in the nasal cavity. The binding of the odorant molecule to the OR triggers a series of biochemical events that lead to the opening of ion channels, creating at first a generator potential that, if the latter reaches threshold, leads to action potential firing. New insights into olfactory transduction introduced new key players and highlighted the necessity to study OSN physiology in an OR-dependent fashion.
The necessity of revisiting transduction mechanisms with consideration of the OR that an OSN expresses requires recording methods of odorant responses at single cell levels. A very effective method to do so is the Suction Pipette Technique, which allows the simultaneous recording of the slow receptor current that originates at the cilia and fast action potentials fired by the cell body. This method can be used in combination with gene targeting and editing techniques to fully address important aspects of the olfactory physiology.
Key wordsOlfactory sensory neurons Receptor current Action potential Electrophysiology Suction pipette Single cell recordings Fast perfusion changes
Rita Levi Montalcini Award from Italian Ministry of Education, University, and Research (DM100915_685 to Michele Dibattista).
- 4.Boccaccio A, Sagheddu C, Menini A (2011) Flash photolysis of caged compounds in the cilia of olfactory sensory neurons. J Vis Exp 29(55):e3195Google Scholar
- 5.Ponissery Saidu S, Dibattista M, Matthews HR et al (2012) Odorant-induced responses recorded from olfactory receptor neurons using the suction pipette technique. J Vis Exp 5(62):e3862Google Scholar
- 7.Wang J-S, Kefalov VJ (2010) Single-cell suction recordings from mouse cone photoreceptors. J Vis Exp 5(35):1681Google Scholar
- 11.Cygnar KD, Stephan AB, Zhao H (2010) Analyzing responses of mouse olfactory sensory neurons using the air-phase electroolfactogram recording. J Vis Exp 2(37):1850Google Scholar