Wellcome Open Research
Schistosomiasis is an important parasitic disease, touching roughly 200 million people worldwide. The causative agents are different Schistosoma species. Schistosomes have a complex life cycle, with a freshwater snail as intermediate host. After infection, sporocysts develop inside the snail host and give rise to human dwelling larvae. We present here a detailed step-by-step video instruction in English, French, Spanish and Portuguese that shows how these sporocysts can be manipulated and transferred from one snail to another. This procedure provides a technical basis for different types of ex vivo modifications, such as those used in functional genomics studies.
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Mouahid, G., Rognon, A., de Carvalho Augusto, R., Driguez, P., Geyer, K., Karinshak, S., Luviano, N., Mann, V., Quack, T., Rawlinson, K., Wendt, G., Grunau, C., & Moné, H. (2018). Transplantation of schistosome sporocysts between host snails: A video guide.. Wellcome Open Research, 3 (). http://dx.doi.org/10.12688/wellcomeopenres.13488.1