The Cheshire TB Eradication Group has been set up after a rise in cases of the disease in the county from 30 in 2005 to 116 in 2012.
Chester farmer Richard Fair said the county was the "final frontier" to stop the disease travelling north.
Wildlife groups have been campaigning to stop culls of badgers, which spread the disease.
The National Farmers' Union (NFU) said if trial culls in the South West successfully controlled the spread of TB they could be used in other areas where the disease was endemic, but vaccinations and other methods would also be used.
Mr Fair, who has lost five of his diary herd recently to TB, said: "Tackling the disease in Cheshire is seen as critical if the spread north is first to be stopped and then pushed back.
"It is hoped that by bringing together everyone who has an interest in stopping the spread of this disease, we may really get a handle on where the disease is and work together to stop it in its tracks."
The group's first move is to work with the University of Liverpool on a survey of TB in Cheshire's wildlife to focus badger vaccination effectively.
An NFU spokesman said: "Culls will only ever be carried out in areas where TB is endemic and will never be carried out nationwide.
"Although the problem in the county is clearly getting worse, thankfully there is no evidence of wildlife infection."
Earlier this week environment secretary Owen Patterson called the issue "the most pressing animal health problem in the UK" and set out the government's long-term plans to eradicate it.
Plans include a controversial cull of badgers, due to start this year in two areas of South West England.
The draft strategy published on Thursday said "further research into alternative population control methods (eg sett-based culling methods and non-lethal methods) is also under consideration".

The action sports game, based on Mexican 'Lucha Libre' style wrestling, sees players compete in arenas used by official administrative body the AAA (Asistencia AsesorÃ­a y AdministraciÃ³n) and other traditional Mexican settings.
Traditional Mexican wrestling is a fast-paced sport, with competitors donning colourful costumes, masks and make up.
Popular in Mexico for more than 100 years, the sport has expanded into the United States and has started to gain fans across the globe.
Players of the video game have 30 wrestling characters to choose from.
As is the case in real life wrestling, the aim of the game is both to win and to avoid humiliation - in the Mask vs. Hair contest the loser either has their head shaved on stage or forfeits their trademark wrestling mask for life; the ultimate symbol of pride for many real Lucha Libre wrestlers.
To mark the launch, Hispanic publisher Slang staged a real life Lucha Libre event in the middle of E3. The winner was Dr. Wagner Jr, and his prize was to become the main image on the game's packaging.
Due for release in August this year on all console and hand-held formats, the game will ship with a single player campaign that will let players customise and improve their character, coupled with an online match-up that sees users enter forfeit matches, such as Mask vs. Hair.
Mexican wrestlers have appeared in video games such as Tekken and Street Fighter IV before, but this is the first time that a game dedicated to Lucha Libra has been released.
The developers say that future editions are already in the works.
Farmers and wildlife campaigners have joined together to try to stop the spread of TB in cattle in Cheshire.
[NEXT_CONCEPT]
One of the more unusual titles at E3, the worlds largest video games exhibition held each year in Los Angeles, is Konami's Lucha Libre AAA: Heroes del Ring.