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Are you finding it difficult to have that last cigarette? With your motivation and determination, hypnotherapy can help you to stop smoking.
If you find yourself worrying about "What if?", hypnotherapy can help you to relax, increase your confidence and learn to deal with anxiety.
Hypnotherapy can help in a wide variety of situations where you might need a little extra confidence such as public speaking, exams and interviews.
If you're finding weight loss difficult, hypnotherapy can help you to develop healthier eating patterns, increase exercise and lose weight.
If you have a fear or phobia, hypnotherapy can help to release the feelings of anxiety and panic, enabling you to stay calm and in control.
Feeling stressed or tired? If you need to relax, de-stress and unwind, these relaxation hypnosis downloads could be just what you are looking for.
Would you like more confidence in life and social situations? Hypnotherapy can help you to increase your self esteem and feel better about yourself.
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Choose from a range of professional hypnotherapy mp3 audio tracks to help with stopping smoking, losing weight, fears and phobias, anxiety, panic attacks, self confidence, public speaking, insomnia, sleep problems and many more.
Take a look at the sections below to find your hypnosis download.
Listen to this free relaxation track and discover hypnosis for yourself.
These hypnosis audio tracks have been written and recorded by an experienced UK based hypnotherapist to help you with a variety of problems and issues. Each hypnotherapy session is supplied as an mp3 file, so you can listen to them on your computer, iPod, mp3 player, iPhone, smartphone, iPad or tablet PC.
Hypnotherapy Downloads provides a range of hypnosis audio tracks available as mp3 files to help in a variety of life situations. Produced by UK based hypnotherapist Vicki Crane, each track is designed to work with your subconscious mind and enable you to make positive changes in your life.
Hypnotherapy can help in a wide variety of life situations. During a hypnotherapy session, hypnosis can be used to suggest new thought or behaviour patterns and when combined with your motivation and participation, can enable new and positive changes to take place.
The various problems and situations that people face are just as unique as the people who experience them. Hypnotherapy Downloads also offers personalised hypnosis audio tracks which are written and recorded just for you, based on your requirements, experiences and goals.


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The answers to all your questions about CBD.
Below are all the questions we’ve answered about CBD!
You can also learn more about CBD here!
Are CBD Gummies Legal To Have In The United States?
Why Are You Gaining Weight With CBD Oil?
How To Buy CBD Oil In India?
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Why Is Full Spectrum CBD Making Your Anxiety Worse: Explanation And Alternatives


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ProstatEase ™ is a clinically researched formula that unblocks painful stagnation in swollen prostate, reduces inflammation and clears toxins. May reduce prostate function against aging.†
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Suggested Use: Take 2-4 capsules 3 times daily or as directed by your health care provider. For best results, take at least 30 minutes before or after a meal. For fast-acting relief, open the capsules & mix with 2-3 oz. of water.
100 Vegetarian Caps, 1 Cap=4000mg raw, 10:1 Concentrated, Zero Filler
CAUTION: If pregnant, please consult a physician prior to use. KEEP OUT OF REACH OF CHILDREN.


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PEMF Therapy is a way to recharge every cell in your body so your cells are better able to uptake nutrients, excrete waste, and function optimally. The number one sign that your cells need energy support is pain. Pain and fatigue are your body’s cry for help — it’s how your body gets your attention. Other signs include dis-ease, weakness, reduced function of any body part, and emotional imbalances.
Your body benefits from coming into direct contact with the earth’s magnetic field — it’s one of the reasons people love walking on the beach so much. The ocean’s salt water is an excellent conductor of energy that increases the transfer of energy from earth to body.
The earth’s electromagnetic field (EMF) is safe and beneficial. However, modern technology like mobile phones, Wi-Fi, Bluetooth, computer screens, and high-tension wires produce an EMF at an extreme level that bombards every cell in your body with detrimental energy.
Each cell in your body is like a battery, capable of holding only so much energy. Think of your cells like a mobile phone — over time they lose their charge and need to be recharged to function. There are various reasons your cells may not be generating or maintaining enough charge: physical injury, toxicity, scar tissue, inflammation, and poor nutrition are just some things that suck the life out of your cellular batteries.
The FDA started approving PEMF back in 1979 for bone loss (it was even used by NASA to support the astronauts when they returned to Earth). The FDA made additional approvals in 1987 for post-operative edema and pain, 2004 as an adjunct to cervical fusion surgery, and 2011 for depression.
Since every cell in your body needs to hold a charge, every cell in your body can benefit from PEMF.
Many people are now discovering the amazing results they can achieve with PEMF.
Bogart proves there’s no placebo effect!
Bogart's miraculous recovery
Discover what PEMF therapy can do for you.
Book an appointment now!
PEMF Therapy Options
We provide three PEMF therapies to choose from
Premium Pro Starter Pack
Chair & Extremity Therapy
Premium Pro Starter Pack
If this is your first PEMF therapy session, start with 3-hour Premium Pro Starter Pack. Package includes three 1-hour appointments and orientation. Based on your case and how you respond to the therapy, we will suggest the package or membership options that are best suited for your wellness goals.
3-hour Premium Pro Starter Pack — First Appointments: $99
After your 3-hour Premium Pro Starter Pack appointments, you may select from the following Pro packages.
30-minute Session: $30
1-hour Happy Zap at 7.8 Hz: $45
1-hour Session: $50
6 Pack of 1-hour Sessions: $250 (save $50)
12 Pack of 30-minute Sessions: $300 (save $60)
12 Pack of 1-hour Sessions: $400 (save $200)
24 Pack of 1-hour Sessions: $750 (Save $450)
30 Pack of 1-hour Sessions: $950 (Save $550)
Chair & Extremity Therapy
Chair & Extremity PEMF therapy may be a good next step for some clients after they complete their three 1-hour introductory sessions.
The intensity can be adjusted to a level most comfortable to you — minimizing detox reactions — and be focused more on specific parts of your body.
This therapy is also the best choice if you benefit from specific brainwave frequencies.
12 1-hour Sessions: $400 ($33 each)
30-minute Session: $30
1-hour Session: $50
What to Expect from PEMF
Pulse Electromagnetic Field (PEMF) Cellular Exercise is a therapy in which electromagnetic fields reach deep inside your body to exercise your cells. It causes your cells to expand and contract, similarly to when you work out at the gym. Just like your muscles, your cells need to be strong. Weak cells are characterized by low voltage. PEMF Cellular Exercise replenishes energy in low voltage cells. As your cells expand and contract, they are able to expel toxins and absorb nutrients. Your cells’ voltage increases and they grow stronger. High voltage cells are able to grow, heal, and reproduce successfully. Strong cells will create more strong cells!
PEMF Cellular Exercise has also been found to support the body's own healing ability by replenishing the natural charge that exists in healthy cells. When your body is healthy you sleep better, experience more energy, and are less likely to experience pain and inflammation.
The basic technology has existed in various forms for decades, starting with Nikola Tesla’s alternating current in the 1800’s. About seventy years ago, Dr. Harold Saxton Burr, PhD proved that disease was preceded by a quantifiable imbalance in our electrical charge. Shortly after, PEMF Cellular Exercise began being used to aid in bone growth. It currently is used to support whole body wellness.
People of all ages can benefit from PEMF therapy. There are no age restrictions. Senior citizens particularly enjoy PEMF, but regardless of age, your cell love a charge!
No, you will feel a gentle pulsing or tapping. Many times our clients doze off during their sessions.
Absolutely! This therapy charges your cells and allows them to function at their fullest capacity. Experiments conducted at Old Dominion University and supported by the U.S. Air Force Office of Scientific Research observed the effects of ultra-powerful pulses on living tissue. As an emerging field of study, Bioelectrics, reveal "these powerful, ultrashort voltage pulses do something nothing else can — harmlessly slip past the cell’s exterior to shock the vital structures within."
If you have a pacemaker, are pregnant, have a history of seizure, or have any electronic device implanted in your body (that cannot be removed for the session), PEMF is not an option for you. Please ask us about other appointment types that may be helpful.
No, all PEMF therapy can be done while you are fully clothed. You are welcome to remove your shoes, if you like.
Depending on your wellness challenges, we may recommend 30, 60, or 90 minute sessions. The best place to start is with three, 1-hour sessions within 10 days or less.
This will be determined through your intake assessment. Different problems require different amounts of treatment. We have affordable packages available.
Since the effects are cumulative, it is recommend, when your first start PEMF, that you come at least twice a week, but you can come every day if that works for you. After your initial series of appointments, we offer packages and maintenance plans with weekly or monthly sessions — depending on your case.
No. While many of our clients come to us at the recommendation of their physician, a referral is not required.
The Pulse XL Pro is the only Cellular Exercise system in the world to combine solid-state engineering, integrated electronics, and user-controlled adjustable pulse and magnetic field strength. We combine the PEMF table, chair, pads, paddles, and rings to create a wellness program that is focused on your specific needs!
In order to accommodate both urgent matters and clients who don’t require the specificity and intensity of the XL-Pro model, we also offer the Chair & Extremity PEMF level of therapy. Learn more about Chair & Extremity PEMF.
Turbo NUtrients is a 100% carbon-bond liquid trace mineral supplement, an all-in-one multivitamin enhanced with humic and fulvic acids. Humic and fulvic acids assist in expelling toxins from your cells. We recommend combining Turbo NUtrients, Drainage Tone, and ReHydration, to your water before and after a PEMF Therapy session to support your cells through the detox process.
Most people report feeling great! If you feel tired or achy after your session, that may be an indication that many toxins were released from your cells during your session. Taking the Turbo NUtrients/Drainage-Tone/ReHydration combination (in water) should reduce or eliminate this. Please let your technician know so that your next session can be gentler. If you feel bad after your session, call us right away for a complimentary "low & slow" where we will support your body with the detox process with 15-20 minutes in our PEMF chair.
That's no problem. Even if you have metal screws, plates, rods, or joints, you can still participate in PEMF therapy.
Eating a clean, healthy diet that includes raw, fresh vegetables and fruits and being well hydrated are a great way to support any wellness program. We also recommend the Turbo NUtrients/Drainage-Tone/ReHydration combination before and after your session. Across the board, we always recommend chiropractic care to support total body healing. When your cells and your brain can talk to each other and energy if flowing optimally throughout your nervous system, any therapy you try can be more effective.
Yes, the FDA first approved PEMF for bone loss in 1979 and over the years, has continued to add more indications.
We are not an approved provider for any insurance company. You could check with your insurance company to see if they will reimburse you for part or all of your sessions. You can also check with administrator of your Health Savings Account to see if those funds can be used for your PEMF sessions.
You can book an appointment on our website or call the office at (661) 940-6302.


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A first in the aesthetics industry, the Radara™ system consists of a unique micro-channelling transdermal patch with a hyaluronic serum, that is designed to improve skin quality and reduce the visible signs of ageing.
The Radara skin care regime was launched in the UK in the autumn of 2015, by Swansea-based Innoture Medical Technology Ltd. It has been designed, developed and manufactured by Innoture Medical Technology Ltd with research directed by leading skincare advisors based at the state-of-the-art Institute of Life Science at Swansea University.
Radara stands for skin Radiance and beauty (adara is Greek for beautiful) and provides the ideal combination of nature and science; giving skin back its natural hydration, support and luminosity as well as gently reducing fine lines and wrinkles.
This innovative, targeted and painless approach to gentle skin rejuvenation delivers smoother, firmer skin with reduced lines and wrinkles in just four weeks according to their latest trials. A patch for the eye area is the first to become available for this technology.
Intended for once-daily home use, Radara is available through selected medical aesthetic clinics, to ensure that you receive guidance on safe and effective use from qualified practitioners.
The Radara skincare system consists of their innovative micro-channelling patches, and the active serum, containing a high purity, naturally-derived hyaluronic acid (HA) serum which is comprised of both a low and medium molecular weight HA.
The low weight HA is absorbed rapidly into the skin and the higher molecular weight HA is being used to thicken theserum, rather than having to use another ingredient within the product.
Designed for use around the outer eye area (for lateral canthal lines or crow’s feet), the cleverly shaped, flexible patches are coated with 2,000 microscopic plastic structures (similar to tiny needles) which are less than half a millimetre in length that are deposit-printed onto the patch.
The plastic structures on Radara patches are specifically designed to gently create micro-channels into the skin, down through the stratum corneum and epidermis. These microchannels quickly heal, meaning that Radara can be used on a daily basis for a number of weeks.
The skincare system uses a mild micro-needling technique that delivers active serums deep into the skin, without actual needles, pain, or recovery time.
Over time the skin produces less hyaluronic acid, which causes a loss of elasticity and hydration. This results in the skin looking more aged, with fine lines and wrinkles, and skin sagging starting to appear.
When applied to the skin, nightly after cleansing, these patches painlessly create hundreds of tiny micro-channels in the epidermis. Radara’s specially-formulated HA serum is then applied (0.2ml delivered per pump action for each side of the face), and flows through the newly created micro-channels in the skin barrier to the deeper skin layers where it restores and replenishes the natural elasticity, hydration and support. The patch is then reapplied over the serum and left for a further five minutes. Skin feels smoother and firmer, and signs of ageing such as fine lines and wrinkles are gently reduced.
Cosmetic, not a medical device. Radara micro-channelling patches are manufactured from a medical grade polymer which is ISO 10993 biocompatibility tested. The materials used to create the patches are all U.S. FDA approved for human use in clinical applications and currently “in use” within medical device components.
The Radara system offers a painless approach to skin rejuvenation of the face, specifically around the outer eye area, for crow’s feet. The system improves the appearance of fine lines and wrinkles, plumps the skin, and improves skin quality, with better hydration and elasticity.
Radara is a one-month regimen consisting of 30 pairs of rejuvenating, single-use, micro-channelling patches and a high purity, naturally-derived hyaluronic acid (HA) serum, provided in a separate pump.
This rejuvenating treatment is only available to purchase through approved aesthetic or cosmetic skin clinics.
All clients should undergo a consultation with an approved Radara skincare specialist before going ahead with treatment. It may not be suitable for use in those with certain skincare conditions, or for those with underlying conditions. Those with a known sensitivity to hyaluronic acid will not be able to use the system.
Women who are pregnant or breast-feeding should consult with their GP before undergoing any aesthetic treatment regime, including Radara.
Radara is not recommended for use to boost the effect of any other topically applied products, due to the complex additives such as dyes or fragrances which may be used; you should only use it in conjunction with the supplied HA serum.
Positive effects can be seen within two weeks of treatment, and with optimum results being visible following the fourth week of a treatment plan. The results last for four weeks following treatment, and it is therefore recommended that Radara be used every two to three months to achieve optimum results.
Hyaluronic acid is a natural skincare ingredient, with many years of use. Reported side effects and adverse reactions are rare. Furthermore, the micro-channelling patches do not cause any long-term damage to the skin. The micro-channels heal very quickly, which means they can be used daily, for several weeks.
If you experience any adverse effects when using Radara, you should cease treatment and contact your clinician as soon as possible.
A one month supply of Radara costs approximately £199.
An 8-week trial independently assessed by clinical dermatologists showed:
- Significant reduction in fine lines and wrinkles of up to 35% in four weeks, with noticeable improvements in just two weeks (depending on starting skin condition).
- Radara patches and hyaluronic acid serum were almost twice as effective at reducing fine lines and wrinkles compared to using the serum alone.
- Radara continued to deliver results for up to four weeks’ post-treatment (improved skin quality, wrinkle reduction).
- Five-minute daily application with no downtime or adverse side effects - unlike traditional micro-needling, which may require pre-treatment anaesthetic and can lead to bleeding.
The before and after images are of real users, and your results may vary. Your practitioner will be able to discuss your expected results with you in your consultation.
Innoture Aesthetics – Data on File.
Specialist dermatological report on the optical 3D-Measurement of the surface of the skin Quantitative evaluation of the roughness of the surface of the skin with the calculation of standardized skin roughness parameters according to DIN 4768ff. Dermatest® GmbH I Engelstrasse 37 I 48143 Münster
In vitro penetration studies and biocompatibility of microneedle array-based delivery systems. Project Report, Queens University, Belfast


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How do you make a Stress Away roll?
Ingredients for roll-on essential oil blend:
- 2 teaspoons grape seed oil.
- 4 drops lavender essential oil.
- 3 drops bergamot essential oil (Bergaptene-free)
- 2 drops Roman chamomile essential oil.
- 1 drop geranium essential oil.
What can I mix Stress Away with?
Aroma and Blending
- The aroma of Stress Away is sweet and tropical.
- Stress Away blends well with single oils like lime, lavender, peppermint, and even cinnamon bark.
- Apply Stress Away with a carrier oil for a more long-lasting effect on the skin.
What essential oils make up Stress Away?
Stress Away Essential Oil Blend. Stress Away™ essential oil blend features Copaiba, Lime, Cedarwood, Vanilla, Ocotea, and Lavender for an exotic aroma used for exactly for what the name says and for achieving a happy and po…
Can you mix Stress Away and lavender?
15. Sleep-Spray – Combine oils that you and your kids find relaxing and spray them on pillows for bedtime or nap time! Stress Away is a great idea for this; combined with lavender it’ll calm kids right down and have them snoozing worry-free.
Can you roll Stress Away?
Young Living’s Stress Away Essential Oil Roll-On is the perfect on-the-go natural solution to combat normal stresses that creep into everyday life. This unique blend of vanilla, lime, and other essential oils makes relieving daily stress convenient and easy….Young Living Stress Away Roll-On.
Where do you apply Stress Away roll on?
- Apply it to the bottoms of your feet, the back of your neck, wrists, temples, or any desired area as needed.
- Apply it to your wrists while at work or school to enjoy this blend’s soothing, fresh scent.
- Apply it topically to enjoy a calming and peaceful aroma.
Can I put Stress Away directly on my skin?
Stress Away can even be used topically, and aromatically. It is a blend of Copaiba, Lime, Cedarwood, Vanilla, Ocotea, and Lavender essential oils.
What does Stress Away smell like?
Stress Away is unique because it’s the only Young Living essential oil blend that includes Vanilla. The extra sweetness of Vanilla makes this essential oil great for adding a sugar, sweet aroma to blends that smell like you just pulled a sweet treat out of the oven.
Is Stress Away good?
Great product I definitely recommend this, it is a great product and makes a huge difference. very helpful Its been like 3 days using them and I’m already feeling the difference.. I feel less anxious and stressful and plus I now sleep better.
What is doTERRA’s version of Stress Away?
Compare Essential Oils
|Plant Therapy®^||Young Living®†||doTERRA®*|
|Worry Free||Stress Away||Serenity®*|
|Worry Free||Trauma Life|
Does Stress Away help you sleep?
Stress Relief Techniques to the Rescue. “Activities that switch on the body’s natural relaxation response feel great,” Buenaver says. “And they have been proven by research to improve sleep. They help by reducing the release of the stress hormones cortisol and adrenaline and by slowing your heart rate and breathing.
Can you overdose on Stress Away?
Symptoms of overdose may include: stomach pain, nausea, vomiting, diarrhea. If your doctor has prescribed this medication, do not share it with others. Keep all regular medical and laboratory appointments.
How do I make stress away?
This recipe will make 100 drops worth (5ml) of Stress Away. Feel free to multiply as needed for a larger batch. 1. Simply add each essential oil to an empty 5ml essential oil bottle. 2. Cap closed and roll between your palms to mix. 3. Allow the blend to “synergize” for a few days if possible to let the oils meld.
How do you make a stress away essential oil blend?
Loving Preparation – Stress Away Recipe 1 Simply add each essential oil to an empty 5ml essential oil bottle. 2 Cap closed and roll between your palms to mix. 3 Allow the blend to “synergize” for a few days if possible to let the oils meld…
What are the best essential oil roller bottle recipes?
20 Essential Oil Roller Bottle Recipes 1 Upset Tummy Roller. Top off a 5 mL bottle with sweet almond oil or fractionated coconut oil! 2 Motivation Roller Blend. Top off a 5 or 10 mL bottle with almond oil,… 3 Muscle Rub Roller Blend. I like this one for my sore achy muscles! 4 Itch Stick Roller Blend. This is a great one for summer time…
How do I make my own stress away 5ml bottle?
Here’s what you need to make your own 5ml bottle of Stress Away: • 19 drops lime essential oil (steam distilled is recommended. This recipe will make 100 drops worth (5ml) of Stress Away. Feel free to multiply as needed for a larger batch.


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Cost: Free with in-app upgrades available
Available for Apple and Android
This app allows you to track your blood glucose values, either by manually inputting them or using their special cable (additional cost) to upload them from your glucometer. You can add notes to each entry to include medication, exercise, meals, and mood. You can use the app to track other readings, such as blood pressure, weight, and A1C.


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Green coffee bean extract and caffeine sensitivity - How to lose weight at 52 years old
Green coffee bean extract and caffeine sensitivity. The document has moved ffee is one of the most popular beverages in the world.
Customer reviews & more. Hydroxycut SX- 7 Revolution Review plus results benefits. While many drink coffee to gain increased mental alertness energy from its caffeine content, some prefer to avoid caffeine ( 1 2). Moved Permanently. What are Hydroxycut SX- 7 Revolution ingredients?


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The first thing that needs to be recognized is that there is a formula to losing weight, and it’s a very simple one: you must take in fewer calories than you put out. Therefore, if you need 2,000 calories to maintain your weight, you are going to have to consume less.
There is no magic pill or shake or diet that will allow you to lose weight taking in more calories than you need. People are always looking for a quick solution, and they rarely—if ever—have lasting results. So losing weight is a balancing act.
The Role of Protein in Weight Loss
How much protein you eat each day is the single most important calorie consideration you’ll make in regards to influencing your metabolic rate. More protein means more weight loss. You need quality protein in order to sustain muscle during weight loss, build HDL cholesterol, enhance insulin and leptin function, improve muscle fitness, antioxidant function, and your immune system.
All of these are necessary to realize optimal weight management attempts over time.
How Much Protein Is Enough for Weight Loss?
For optimal weight loss, scientific evidence suggests that you need levels far higher than the government’s recommended level. But carbohydrate intake must be reduced at the same time. Ideally, you will take in a minimum of three fourths of your ideal body weight in grams of protein every day up to three fourths of your actual weight in grams of protein every day. For instance, a person who wants to weigh 150 pounds but actually weighs 190 pounds will need a minimum of 112 to 142 grams of protein per day.
What About High-Protein Weight Loss Shakes?
Unfortunately, high-protein weight loss shakes are usually high in sugar as well, a carbohydrate that you definitely want to avoid in your weight loss journey. But not all high-protein shakes are created equal. Muscle Milk, a popular high-protein, low sugar, ready to drink product or protein powder supplement that is made for those who want more protein in their diets.
With just 6 grams of sugar and 32 grams of protein, these shakes are extremely well-calculated to add protein and limit carbohydrates of the kind that are enemies of weight loss. However, at 350 calories per shake, it is difficult to cut enough calories to sustain weight loss. Low calorie foods and drinks are necessary to create a calorie deficit, which is why Muscle Milk Light is a better choice for weight loss.
In order to boost metabolism and feel full, protein is the most important ingredient. But you need healthy carbs to make up the rest of your diet, too. That’s why Muscle Milk Light might be a better choice for those trying to lose weight. At just 170 calories per shake and 20 grams of protein, it makes a hearty snack but without the excess calories other shakes contain.
One way of using Muscle Milk for weight loss is to use the shakes as a meal replacement. The other way is to use Muscle Milk Light for snacks and eat fewer calories during meals.
What Does Research Tell Us?
Research has shown that whey protein is the way to go to lose weight. In a study where overweight people were given protein shakes with equal calories, the group that consumed whey protein lost weight and those on soy did not.
Unfortunately, Muscle Milk does not contain whey protein, but gets its protein from milk. It also contains heavy metals in quantities that may not be safe. It is highly processed, and is artificially sweetened with Acesulfame Potassium, which has been shown to cause cancer in laboratory studies.
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text:
Patent application title: USE OF THERAPEUTICALLY USEFUL PEPTIDES
Tiina Jauhiainen (Helsinki, FI)
Riitta Korpela (Helsinki, FI)
Heikki Vapaatalo (Tamper, FI)
Mats Ronnback (Helsinki, FI)
IPC8 Class: AA61K3806FI
Class name: Designated organic active ingredient containing (doai) peptide containing (e.g., protein, peptones, fibrinogen, etc.) doai 3 or 4 peptide repeating units in known peptide chain
Publication date: 2009-04-30
Patent application number: 20090111758
The invention relates to preventing and treating endothelial dysfunction
by using biologically active peptides and products containing them. In
particular, the tripeptides Ile-Pro-Pro (IPP), Val-Pro-Pro (VPP) or
mixtures, concentrates or other products containing them are used. A
specific aspect of the present invention is to enhance the elasticity of
blood vessels by using said biologically active peptides.
1. Use of lie-Pro-Pro and/or Val-Pro-Pro for the preparation of a product
improving endothelial function.
2. The use according to claim 1, characterized by using a product comprising lie-Pro-Pro and/or Val-Pro-Pro for the preparation of a product improving endothelial function.
3. The use according to claim 2 of a product that has a high content of casein-derived, small-molecular peptides and has been prepared by fermenting a casein-containing starting material with a lactic acid bacterium for the preparation of a product improving endothelial function.
4. The use according to claim 3 of a product that has a high content of casein-derived, small-molecular peptides and has been prepared by fermenting a casein-containing starting material with Lactobacillus helveticus strain LBK-16H, DSM 13137, or Lactobacillus helveticus strain LB 1936, DSM 17754, optionally removing partly or entirely the casein and/or other milk proteins and/or lactose, and nanofiltering the resulting peptide-containing fermented product, for the preparation of a product improving endothelial function.
5. The use according to claim 2 of a soured composition containing the casein-derived, small-molecular peptide Ile-Pro-Pro and/or Val-Pro-Pro, and living lactic acid bacterium for the preparation of a product improving endothelial function.
6. The use of a product according to claim 3, characterized in that the casein-derived, small-molecular peptides comprise a mixture of short-chain peptides.
7. Use according to claim 1 for the preparation of a medical product for prevention or treatment of disorders and diseases relating to endothelial dysfunction.
8. Use according to claim 1, characterized in that the product enhances the elasticity of blood vessels.
9. Use according to claim 1, characterized in that the end product is an edible product.
10. Use according to claim 9, characterized in that the end product is a product of dairy industry, beverage industry, processed food industry, processed meat industry, baking industry or confectionery industry.
11. Use according to claim 10, characterized in that the end product is a fermented milk product.
12. Use according to claim 10, characterized in that the end product is a beverage, preferably a whey beverage, a fruit beverage or a beer.
13. Use according to claim 9, characterized in that the end product is a product of pharmaceutical industry.
14. A method for normalizing endothelial dysfunction, comprising administering to an individual a peptide selected from the group consisting of lie-Pro-Pro and Val-Pro-Pro and mixtures thereof, or a product containing said peptide(s), in a sufficient amount to achieve the desired effect.
15. A method for prevention or treatment of endothelial dysfunction, and disorders and diseases relating thereto, by administering to an individual a peptide selected from the group consisting of lie-Pro-Pro and Val-Pro-Pro and mixtures thereof, or a product containing said peptide(s), in a sufficient amount to achieve the desired effect.
FIELD OF THE INVENTION
The present invention relates to preventing and treating endothelial dysfunction by using biologically active peptides and products containing them. A product having a high short-chain peptide content has been found especially effective for use in accordance with the present invention.
The product to be used in accordance with the present invention can be formulated for instance as a health and wellness food product or a pharmaceutical product. It contains small-molecular peptides, such as the tripeptides Ile-Pro-Pro (IPP), Val-Pro-Pro (VPP), or mixtures or concentrates containing them, and it functions by improving epithelial function and curing diseases relating thereto. A specific aspect of the present invention is to reduce stiffness and thus enhance elasticity of blood vessels with the use of small-molecular peptides, such as tripeptides Ile-Pro-Pro (IPP), Val-Pro-Pro (VPP), or mixtures, concentrates and other products containing the same.
BACKGROUND OF THE INVENTION
The vascular endothelium regulates locally vascular tone by the release of vasodilator substances, such as endothelium-derived relaxing factor (EDRF), nitric oxide, prostacyclin and endothelium-derived hyperpolarizing factor (EDHF) and vasoconstrictor substances, such as thromboxane A2, free radicals and endothelin. The importance of nitric oxide in both basal and stimulated control of vascular tone in large epicardial coronary arteries and in the coronary microcirculation has been shown in several clinical studies (for review see Vapaatalo H, Mervaala E. Clinically important factors influencing endothelial function. Med Sci Monit 2001; 7(5):1075-1085. Drexler H, Hornig B. Endothelial dysfunction: a novel therapeutic target. J Mol Cell Cardiol 1999; 31:51-60). Above-mentioned different endothelial derived relaxing factors correct endothelial dysfunction e.g. in atherosclerosis. Regulation of vasomotor tone, modulation of blood coagulation, promotion and prevention of vascular growth, modulation of inflammation and action as a target to the effects and adverse effects of drugs are associated to endothelium. EDRF release is stimulated by increased flow in vessels of for example bradykinin, thrombin, acetylcholine and serotonin.
The endothelium controls underlying smooth muscle tone in response to certain pharmacological and physiological stimuli. The endothelial function plays also a role in vascular growth, leukocyte adhesion, and immunological regulation, metabolism of circulating amines, lipoprotein metabolism and integration and transduction of blood-borne signals.
Endothelial dysfunction is characterized by decreased secretion of vasodilatory mediators, increased production of vasoconstrictors, increased sensitivity to vasoconstrictors and/or resistance of vascular smooth muscle to endothelial vasodilators. Dysfunction is a consequence of an imbalance between relaxing and contracting factors, or growth promoting and inhibiting agents. Inflammation, lipoprotein oxidation or other oxidative stress reactions are factors affecting development and maintenance of endothelial dysfunction. Theoretically, the clearest and most direct indicators of endothelial dysfunction are nitric oxide and its metabolites, as well as cyclic GMP.
Endothelial dysfunction can be either a cause or a consequence of several clinical conditions, such as hypertension, atherosclerosis, coronary disease, heart failure, diabetes and high blood cholesterol. The most direct indicators of endothelial dysfunction are imbalance between decreased production or receptor function of vasodilatory factors, such as nitric oxide, prostacyclin, endothelium derived hyperpolarizing factor (EDHF), and natriuretic peptides, or increased formation of or sensitivity to vasoconstrictive agents, such as endothelin-1, angiotensin 11, endoperoxides, and thromboxane A2.
Endothelial dysfunction can be treated with several known drugs, the most important being angiotensin converting enzyme (ACE) inhibitors, angiotensin II receptor blockers, nitrate preparations and cholesterol lowering drugs. The effect of ACE inhibitors is mainly based on their capability to improve the effect of bradykinin, which enhances the synthesis of nitric oxide in endothelium. Induced and/or enhanced nitric oxide production or added nitrates can balance insufficient internal nitric oxide production. The medicines and nitrates function as exogenic EDRF and dilate blood vessels, and in addition they are active as antithrombotic compound in damaged veins.
WO 02/34767 A1, Selwood et al., describes peptides which are fragments of vascular endothelial growth factor (VEGF), and useful for inhibiting angiogenesis. According to the invention, the peptides comprise three to eight amino acids, and a key feature of the amino acid sequence is the presence and arrangement of basic residues, in particular arginine (Arg) and/or lysine (Lys) residues. The document specifies several peptides, consisting of from six to sixteen amino acids. None of them include the sequences Isoleucin-Proline-Proline (IPP) or Valine-Proline-Proline (VPP). According to the publication, the peptides may be useful in diseases where angiogenesis plays a significant role in pathology. Such diseases may include diabetic retinopathy, age-related macular degeneration (ARDS), cancer, endometriosis, psoriasis and rheumatoid arthritis.
WO 99/45941, Sandberg et al., describes a composition used to enhance the softness, elasticity, or appearance of tissue. The composition is formulated from peptides that correspond to any one of 41 peptide fragments produced from thermolysing digestion of elastin. Preferably, the composition comprises a polypeptide having the formula R1-Valyl-Valyl-Prolyl-Glutamine-R2, wherein R1 is an amino portion of the peptide, and R2 is a carboxy portion of the peptide. The composition is preferably applied to human skin in a cosmetic formulation. According to the document, the composition may also be useful for treating hypertension, coronary heart disease, arteriosclerosis, angina, coronary thrombosis, chronic obstructive pulmonary disease, and restenosis post angioplasty.
WO 01/91700 A2 and U.S. Pat. No. 6,962,904 B1, both Mitts et al, are in part based on the same study as WO 99/45941, Sandberg et al. The documents describe compositions for enhancing the elasticity of tissue, and the compositions are formulated from peptides corresponding to sequences found in elastin, in particular the sequences -Valine-Valine-Proline- and -Valine-Valine-Proline-Asparagine-. Said compositions are useful for improving elastin production in tissues. According to the publications, the main utility is once again in cosmetics, but it is also mentioned that the compositions may be useful in treating e.g. hypertension, coronary heart disease, arteriosclerosis, angina, coronary thrombosis, chronic obstructive pulmonary disease and restenosis post-angioplasty.
The described elastin peptide fragments contain a large number of glycine and/or proline residues as compared to other amino acid sequences. The fragments do not include the sequences Isoleucin-Proline-Proline (IPP) and/or Valine-Proline-Proline (VPP).
Preparation of bioactive peptides by fermentation has been largely studied and described in the background art, one particular point of interest being milk-derived peptides. These have been shown to have for instance opioid receptor binding properties, ACE inhibiting activity and antimicrobial properties. Several studies have been made in relation to e.g. hypertension, but effects of the peptides on the endothelial functions have not been described. In particular, effects of the tripeptides Valine-Proline-Proline (VPP) and Isoleusine-Proline-Proline (IPP) and products containing the same on the endothelial functions have not been described in the background art.
The tripeptides VPP and IPP are known compounds, which have been described as ACE inhibitors having an antihypertensive effect. For instance in J Dairy Sci 78 (1995) 777-783, Nakamura et al. describe the use of a starter containing Lactobacillus helveticus and Saccharomyces cerevisiae for the preparation of two ACE inhibitors. The compounds were both identified as tripeptides, Val-Pro-Pro and Ile-Pro-Pro. Although the publication does not describe an in vivo antihypertensive effect of the tripeptides, it is mentioned to be the next subject of research.
U.S. Pat. No. 5,449,661, Nakamura et al., discloses the preparation of a peptide containing the tripeptide sequence Val-Pro-Pro and its use for lowering high blood pressure. The peptide is prepared by fermenting fat-free milk powder with the Lactobacillus helveticus strain JCM-1004, whereafter the peptide is purified chromatographically and freeze-dried.
In WO 99/16862, Yamamoto et al. describe the Lactobacillus helveticus strain CM4, FERM BP-6060, which is capable of producing a large amount of the tripeptides Val-Pro-Pro and/or Ile-Pro-Pro.
WO 01/32905, Valio, describes a product having antihypertensive properties and its preparation. The product is produced by fermenting a casein-containing starting material with a lactic acid bacterium, and performing nanofiltration on the obtained, peptide-containing fermentation product. The antihypertensive properties of the product are in part due to the tripeptides IPP and VPP contained therein. WO 03/070267, Valio, describes the use of IPP and VPP, as well as the product described in WO 01/32905, in the preparation of a product enhancing the availability of minerals. The product can be used e.g. for increasing bone formation, strengthening the skeleton system and for treating or prevention of osteoporosis.
Blood vessels have implications in diseases associated with viscoelasticity, including hypertension, arteriosclerosis, angina, angiogenesis, myocardial infarction, coronary thrombosis, restenosis post angioplasty, and chronic obstructive pulmonary disease. Cardiovascular diseases are amongst the most common diseases in the world, and they are on the top five list of life threatening diseases in many countries. Unfortunately, increasing living standards also increase the risk of said diseases, and hence they will play an even greater role in the future. In addition to conventional drugs, functional products are nowadays providing an attractive alternative to the consumers.
Functional products improving the elasticity of blood vessels and improving and normalizing endothelial function would hence be very welcome as part of a regular diet. Furthermore, administering peptides with beneficial arterial stiffness properties as a medical or pharmaceutical product is also worth considering.
BRIEF DESCRIPTION OF THE INVENTION
It is thus an objective of the present invention to make available a product that as part of a regular diet, or as a medical and pharmaceutical product, improves or normalizes endothelial function and hence is capable of preventing, alleviating or curing disorders and diseases relating to endothelial dysfunction. Endothelial dysfunction has a remarkable role in the stiffness or flexibility of blood vessels, which in turn is important in many severe disorders including e.g. coronary heart disease, arteriosclerosis, angina, coronary thrombosis, chronic obstructive pulmonary disease and restenosis post-angioplasty. Hence, ability to enhance the elasticity of blood vessels is an especially important feature of the present invention.
The product to be used according to the invention consists of or comprises peptides improving endothelial function.
It is a further object of the present invention to make the product available either as such, as an agent improving endothelial function, or for use in the preparation of functional foodstuffs or drugs intended for consumption.
A yet further object of the present invention is to provide a method for prevention, alleviation or cure of endothelial dysfunction, as well as disorders and diseases relating thereto, by administering to an individual in need of such treatment peptides improving endothelial function or a product containing them in a sufficient amount to produce the desired effect.
A yet further object of the present invention is to provide a method for prevention, alleviation or cure of endothelial dysfunction, as well as disorders and diseases relating thereto, by administering to an individual a product that has a high content of casein-derived, small-molecular peptides and that has been prepared by fermenting a casein-containing starting material with Lactobacillus helveticus strain LBK-16H, DSM 13137, or Lactobacillus helveticus strain LB 1936, DSM 17754, and optionally removing partly or entirely casein and/or other milk proteins and/or lactose, in a sufficient amount to produce the desired effect. In a preferred embodiment, the fermented product is also subjected to nanofiltration.
DETAILED DESCRIPTION OF THE INVENTION
According to the present invention it has been surprisingly found that the above objectives are achieved by using small-molecular peptides, in particular the tripeptides Ile-Pro-Pro (IPP), Val-Pro-Pro (VPP). Said peptides have now been proven capable of normalizing endothelial functions, improving the elasticity of blood vessels and combating arterial stiffness.
The peptides used in accordance with the present invention are bioactive peptides corresponding to those derived through hydrolysis of casein or casein-containing material, such as milk. Short-chain di-, tri- and tetrapeptides and their mixtures are considered suitable. In particular, the peptides are the tripeptides IPP and VPP, mixtures of peptides including IPP and VPP, and products and compositions including the same.
The preferred embodiment provides excellent possibilities to normalize endothelial dysfunction and endothelium dependent damages.
The invention thus relates to the use of casein-derived, small-molecular peptides in the preparation of a product improving endothelial function.
The invention further relates to the use of a product comprising casein-derived, small-molecular peptides in the preparation of a product improving endothelial function.
Preferably, the casein-derived, small-molecular peptides comprise di-, tri-, and tetrapeptides and their mixtures. Most preferably, the IPP and VPP contents are high.
The invention further relates to the use of a product having a high content of casein-derived, small-molecular peptides that has been prepared by fermenting casein-containing starting material with a lactic acid bacterium, and possibly by nanofiltration of the fermented peptide-containing product obtained, in the preparation of an end product improving endothelial function.
As a preferred embodiment, the invention relates to the use of a soured composition that contains casein-derived peptides, minerals and living lactic acid bacterium, in the preparation of a product improving endothelial dysfunction.
The invention also relates to a method for normalizing endothelial function and endothelium dependent damages by administering to an individual peptides normalizing endothelial function and endothelium dependent damages or a product containing them in a sufficient amount to produce the desired effect.
The individual is primarily human. The positive effects of the products used in accordance with the invention are naturally also beneficial to animals, especially pets and production animals. Examples of these include dogs, cats, rabbits, horses, cows, pigs, goats, sheep and poultry.
The tripeptides VPP and IPP can be prepared synthetically or by hydrolysis of material containing said sequences. In a preferred embodiment, the peptides are prepared from casein or casein-containing starting material by fermentation. Especially preferable is to use the method described in WO 01/32905, in which a product containing biologically active peptides is formed by fermentation, followed by concentration and nanofiltration. This preferred embodiment provides excellent possibilities to use starting materials of different types and to modify the composition of the end product as desired, as described in detail in WO 01/32905, which is incorporated herein by reference.
When biologically active peptides for use in accordance with the present invention are formed by fermentation, the starting material can be any product that contains the sequences of the desired biologically active peptides as part of its own peptide or protein sequence. Milk protein, especially casein, is preferably used as such or in the form of different preparations. Suitable starting materials also include various casein-containing milk products, such as skimmed milk or milk with varying fat contents as such or in the form of a corresponding milk powder and sour milk products, such as sour milk, buttermilk, yoghurt, curdled milk, unripened cheese, etc.
Fermentation can be carried out with any lactic acid bacterium that is capable of producing the desired peptides from the starting material. Suitable lactic acid bacteria can be found among species belonging to the Lactobacillus, Lactococcus, Leuconostoc, Streptococcus and Bifidobacterium genera, for instance. The most proteolytic of the lactic acid bacteria is Lactobacillus helveticus and it is thus considered especially suitable for this purpose. A preferable Lactobacillus helveticus strain is L. helveticus LBK-16H, DSM 13137, which is described in detail in patent publication WO 01/32836. Especially preferable is Lactobacillus helveticus LB 1936, DSM 17754, which is described in detail in patent application FI20065054.
The lactic acid bacteria can be used as pure cultures or mixed cultures, separately or together with conventionally used and commercially available souring agents. The lactic acid bacteria can also be used together with other micro-organisms.
The fermentation conditions are selected to meet the requirements of the used strain so as to form a sufficient amount of biologically active peptides to produce the desired effect. The selection of suitable conditions, such as temperature, pH and aeration, is part of the know-how of a person skilled in the. Conventionally fermentation is carried out at about 30 to 45° C. The fermentation is allowed to continue until the desired amount of biologically active peptides has been formed. Normally, this takes approximately 20 to 30 hours. A mixture of various peptides is formed during fermentation. When the fermentation continues long enough, mainly relatively small di- and tripeptides, such as Val-Pro-Pro (VPP) and Ile-Pro-Pro (IPP), are obtained. An incubation time of about 22 to 24 hours is preferred.
The cell suspension obtained can be used as such or the peptides can be separated and purified using conventional methods. Concentration, for instance by evaporation or drying the cell suspension partly or completely, such as spreading the suspension on a plate, drying and finally grinding it to a well-preserved dry powder, is a preferable treatment.
Further treatment of the cell suspension by nanofiltration has proven to be an excellent method that enables production of the peptides active in endothelial functions such as arterial stiffness, together with a desired mineral composition in the same product. By selection of the nanofiltration membrane type and process conditions, it is possible to influence the composition of the obtained product.
Nanofiltration is performed up to the desired dry content range, which may be about 20 to 40%, or to the approximate volume concentration ratio of about 5 to 20. By means of nanofiltration, the peptide content of the product increases. The composition of the end products naturally depends on the fermentation conditions used, the optional nanofiltration treatment and possible other pre-treatment and additional treatments.
In accordance with the present invention, the tripeptides IPP and VPP and products containing the same have surprisingly been shown to reduce arterial stiffness and thus improve endothelial dysfunctions. During a ten weeks intervention period the ambulatory arterial stiffness index (AASI) measuring the arterial stiffness improved significantly in the test group receiving a product comprising tripeptides IPP and VPP as compared to the control group; even in the first non-optimised test the index decreased about 9 percent in comparison to the control group. The average AASI at the end of the treatment period were 0.31±0.15 in the Lactobacillus helveticus group and 0.34±0.17 in the control group.
The products described above can be used as such to achieve the desired effect. The products can also be dried and used in the form of a powder or lyophilized preparation. The products can also preferably be used in the preparation of functional foodstuffs or other products. Applications as medical or pharmaceutical products are also possible.
The concept `foodstuff` has a wide meaning in the present publication, covering all consumable products that can be in a solid, jellied or liquid form, and covering both ready-made products and products to which the biologically active peptides or a product containing them are added during consumption as an additive or part of the product. Foodstuffs can for instance be products of dairy industry and beverage industry. Typical products include milk products, such as yoghurt, curdled milk, curd, sour milk, sour whole milk, buttermilk, other sour milk products, unripened cheeses and ripened cheeses, filling of snack bars, etc. Another important group includes beverages, such as whey beverages, fruit beverages, and carbonated beverages.
According to the invention, the biologically active peptides or a product containing them are used in a sufficient amount to achieve the desired effect. When using the product obtained by the two-step method described above, the amount to use depends mainly on the concentration degree of the whey and is for instance 0.1 to 30%, preferably approximately 5 to 15% as calculated from the weight of the end product.
Biologically active peptides or a product containing them can be added to a food product during its preparation or to a finished food product. The food products in question thus contain the desired peptides, or a product containing them, in addition to other components contained in corresponding food products and fully correspond in taste and behaviour with these conventional products.
The invention will be described in detail by means of the following examples. These examples are provided only to illustrate of the invention and should not be considered to limit the scope of protection in any way. The examples set forth and present the beneficial, advantageous, and remedial effect of IPP and VPP as such or combined on arterial stiffness by enhancing endothelial function, as well as preferred end products for administering the bioactive peptides.
Effect of the Bioactive Product on Arterial Stiffness
Arterial stiffness can be measured by using ultrasound equipment or applanation tonometry, or by using ambulatory arterial stiffness index (AASI). AASI is defined as 1 minus the slope of diastolic or systolic pressure during 24-hour ambulatory monitoring, and it is a reliably indicator of arterial stiffness (Li Y, Wang J G, Dolan E, Gao P J, Guo H F, Nawrot T. Ambulatory arterial stiffness index derived from 24-hour ambulatory blood pressure monitoring. Hypertension 2006; 47(3):359-64).
In a randomised placebo-controlled parallel group study, 94 hypertensive patients not receiving any drug treatment were given either Lactobacillus helveticus LBK-16H fermented milk prepared according to Example 2 or a control product, for ten weeks after a four-week run-in period. Twenty-four hour ambulatory blood pressure measurement was performed at the beginning and at the end of intervention period. The average baseline systolic and diastolic blood pressure values were 132.6±9.9/83.0±8.0 mmHg in the Lactobacillus helveticus group and 130.3±9.6/80.2±7.0 mmHg in the control group.
At the end of the ten week intervention period systolic and diastolic blood pressure had decreased more in the Lactobacillus helveticus group compared to the control group. The treatment effect on SBP was -4.1 (95% Cl: -6.6 to -1.8, p<0.001). The treatment effect on DBP was -1.8 (95% Cl: -3.7 to -0.0, p=0.048).
AASI-index was determined by using the ambulatory blood pressure values and analysing these with the method of Li et al., ibid. The AASI index increases linearly with age in both men and women. Small values in AASI index are more favourable than high values and therefore, if the value decreases, the arterial stiffness index is getting better. The average baselines AASI were 0.36±0.15 in the Lactobacillus helveticus group and 0.36±0.17 in the control group. At the end of the treatment period average AASI were 0.31±0.14 in the Lactobacillus helveticus group and 0.34±0.15 in the control group.
During the ten week intervention period the AASI-index improved statistically significantly in the test groups (p=0.043), but not statistically in the control group (p=0.47) (Table 1).
TABLE-US-00001 TABLE 1 Change in AASI index after 10-week intervention period Sig. (2-tailed) Mean Difference Test group Change in 0.043 -0.0427 AASI- index Control Change in 0.474 -0.0195 group AASI- index
Preparation of Products Useful in the Present Invention
Lactobacillus helveticus strain LBK 16-H, DSM 13137, was grown in MRS broth at 37° C. for 24 hours and inoculated into reconstituted milk (10%) to form an inoculum. After two growth cycles, the inoculum (15%) was inoculated into a fermentor medium made up of 9 to 10% skimmed milk powder milk and sterilized at 110° for 10 minutes. Fermentation was performed at 37° C. for 22 to 24 hours under continuous strong agitation. The product (a) can be used as such, in a dry and/or ground form, or the desired peptides can be separated from it using methods known per se.
The process was repeated by using Lactobacillus helveticus LB 1936, DSM 17754, and rich milk or butter milk, respectively, instead of skimmed milk powder in order to produce corresponding products b and c.
Preparation of an End Product Useful in the Present Invention Peptide Concentrate
Sour milk containing peptides active in correcting endothelial dysfunction was prepared by adding about 1% of a peptide concentrate to a commercially available sour milk. The composition of the product is shown in Table 2, which for comparison also shows the composition of a commercially available sour milk product, AB sour milk, produced by Valio Ltd.
For the Evolus® peptide concentrate fermentation was carried out as described in example 2. The cell suspension obtained was separated by centrifugal clarifying. The thus pretreated whey was nanofiltrated through a Nanomax-50 membrane at 40° C. at a pressure of 30 bar. The whey was filtrated until the volume concentration ratio was 9.
TABLE-US-00002 TABLE 2 The composition of an Evolus product and a commercial sour milk product (AB sour milk, Valio Ltd) Nutrition information/100 g (serving 200 g) Evolus AB sour milk energy 260 kJ/61 kcal 220 kJ/53 kcal protein 3.0 g 3.2 g carbohydrate 12 g 4.4 g of which lactose less than 1.0 g* dietary fibre 0 g 0 g fat 0.1 g 2.5 g calcium 150 mg** 120 mg potassium 233 mg 170 mg magnesium 13 mg 11 mg sodium 24 mg 39 mg Tripeptides Ile-pro-pro 1.1 mg 0 Val-pro-pro 1.3 mg 0 *over 80% of the lactose has been enzymatically degraded **25% of recommended daily intake
Preparation of an End Product Useful in the Present Invention Evolus Tehojuoma Fermented Milk Drink
For the Evolus® daily dose Tehojuoma fermented milk drink, fermentation was carried out as described in example 2. Further, 1% of Evolus® peptide concentrate prepared as described in example 3 was added to fermented milk. The product containing the daily requirement of the active ingredient is to be used as such. The nutrition information of the product is shown in Table 3. Further ingredients which fermented milk drink contains are pasteurized skimmed milk, milk protein concentrate, thickeners, sea algae calcium, acidity regulator, souring agents, flavours, vitamins (folic acid, B6, B12) and colour.
TABLE-US-00003 TABLE 3 The composition of Evolus ® Tehojuoma Fermented Milk Drink Nutrition information/100 g EvolusTehojuoma (serving 100 ml) mixed berry Drink energy 260 kJ/61 kcal protein 3.5 g carbohydrate 11 g of which lactose less than 1.0 g* dietary fibre 0 g fat 0.1 g calcium 200 mg** potassium 410 mg magnesium 15.5 mg sodium 38 mg folic acid 66 μg*** vitamin B6 0.66 μg*** vitamin B12 0.33 μg*** *over 80% of the lactose has been enzymatically degraded **25% of recommended daily intake ***33% of recommended daily intake
Preparation of an End Product Useful in the Present Invention Soft Type of Cheese
Soft type cheese, such as fresh cheese, fresh cheese spread and cottage cheese were manufactured with conventional production methods. For example, Evolus® fresh cheese spread was produced by mixing quarg, cream or butter and other ingredients, heat-treated and packed. Evolus® peptide concentrate prepared as described in example 3 was added to quarg-fat mixture. The product containing the daily requirement of the active ingredient is to be used as such.
Patent applications by Riitta Korpela, Helsinki FI
Patent applications by VALIO LTD
Patent applications in class 3 or 4 peptide repeating units in known peptide chain
Patent applications in all subclasses 3 or 4 peptide repeating units in known peptide chain


text:
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|Pantothenic acid (Vitamin B5 as d-Ca pantothenate)||150mg||2500|
|Vitamin C (from magnesium ascorbate)||100mg||125|
|Natural Beta Carotene/Carotenoid Mix (Dunaliella salina)||5mg|
Equiv. to Vitamin A
Providing (typical analysis): Beta Carotene 4.5mg Cryptoxanthin 39µg Alpha Carotene 158µg Zeaxanthin 32µg Lutein 25µg
|Niacin (Vitamin B3 as Niacinamide)||50mg||312|
|Iodine (from organic kelp)||25µg||16.7|
|Magnesium (from citrate and ascorbate)||12mg||3.2|
|Vitamin B6 (pyridoxine HCI)||20mg||1786|
|Vitamin B1 (as thiamine HCl)||25mg||2273|
|Riboflavin (Vitamin B2)||25mg||1786|
|Grape seed extract (95% proanthocyanidin)||20mg|
|Boron (sodium borate)||500µg|
|Vitamin D3 (400iu)||10µg||200|
|Vitamin B12 (Adenosylcobalamin and Methylcobalamin)||50µg||2000|
|in a base of alfalfa, spirulina and bilberry|
|Plant cellulose capsule|
As a food supplement take one capsule daily with food. Do not exceed stated dose unless recommended by your healthcare practitioner. Store in a cool dry place, out of the sight and reach of children. Not to be used during pregnancy or lactation unless recommended by a healthcare practitioner. This is a food supplement, not to be used as a substitute for a varied diet and lifestyle.


text: An enzyme extracted from pineapple stems, bromelain reduces levels of prostaglandins, which are hormones that induce inflammation. Its blend of 16 natural homeopathic ingredients work together to relieve a full-range of nerve pain related symptoms, without the negative side effects sometimes experienced with prescriptions. They maintain healthy metabolism, skin, and muscle tone. Devil’s claw extract is considered safe at the typical dosage of 750 mg (containing 3 percent iridoid glycosides) taken three times daily. As a result, even besides being written in the language of their day which may be antiquated, the recorded effects (indications) may seem very specific or even strange sounding. Said regulation is conducted in the same manner as that of nonprescription OTC drugs, according to The National Center for Complementary and Alternative Medicine (NCCAM). With MS, demyelination (a loss of the nerve's protective layer caused by and responsible for the symptoms of multiple sclerosis) slows response in the nerves and causes them to begin "miscommunicating. In those with the condition, CPSP causes a misinterpretation of stimuli like touch, warmth, and cold. B Vitamins found abundantly in fish, whole grains, potatoes, bananas, lentils, beans, and tempeh are extremely important to nerve health. One study found as much as 55% suffer from "clinically significant pain" at some point following the condition's development. * Indications for temporary symptom relief are based solely on Homeopathic Materia Medica (HPUS) for self-limiting conditions. Natural homeopathic Neuroveen works gently, yet powerfully to target irritated nerves and relieve nerve pain, numbness, sensitivity, burning, tingling, weakness and troubled mobility. Customers are responsible for any additional Customs fees. Here at HelloLife, we stand behind our products. However, effects are genuine and important to list in their entirety, because homeopaths use that information herbs for nerve pain relief to select the best ingredients for a patient's symptoms. They support cell growth and division and, when deficiency occurs, a lack of B vitamins can cause neuropathic food diet for gastric problems symptoms. A higher concentration of . This herb eases muscular tension or pain in the back, shoulders and neck. It should not be taken with blood-thinning medications and may not be safe during pregnancy or for young children, nursing mothers and individuals with liver or kidney disease, or digestive system ulcers. 025 percent capsaicin can significantly reduce osteoarthritic pain when applied to joints four times daily. As a long-standing approach to medicine with roots back over a hundred years, the traditional language of homeopathy can, understandably, be somewhat confusing for people today. It manipulates the body’s pain status by hindering pain perception, herbs for nerve pain relief triggering the release of pain-relieving endorphins and providing analgesic action. herbs for nerve pain relief When using topical capsaicin products, be sure to avoid touching your eyes and other sensitive areas. (More on the production and action of homeopathics HERE) More rare, affecting just 8% of stroke survivors, is central post-stroke pain or CPSP (also known as thalamic syndrome or thalamic pain syndrome) which occurs with irritation to the brain itself. With Mother Earth Living by your side, you’ll discover all do i have third type diabetes the best and latest information you want on choosing natural remedies and practicing preventive medicine; cooking with a nutritious and whole-food focus; creating a nontoxic herbs for nerve pain relief home; and gardening for food, wellness and enjoyment. A popular treatment for osteoarthritic pain, it may ease rheumatoid arthritic pain as well. Pain is an unfortunately common problem among Multiple Sclerosis (MS) sufferers. Rheumatoid arthritic pain may also be reduced and mobility enhanced within about two months. Neuroveen is manufactured and packaged following the FDA's Current Good Manufacturing Practices (CGMP). We use multi-ingredient blends for greater symptom coverage; multiple potencies for short, mid and long term relief; liquid formulations for greater ease of use; and a purified, ionized mineral water base for optimal safety and delivery in the body. Similar to the way vaccines work, very small, safe doses of these natural ingredients are used to improve immunity and resistance to the problem symptoms people experience. Commercial capsaicin-containing creams such as Zostrix, Heet and Capzasin-P are used topically for arthritic and nerve pain. Homeopathic indications and ingredient names are the same as those originally recorded by the leading forefathers of the practice. We ship via FedEx® or USPS to all international addresses. " Sometimes, this miscommunication takes the form of strange or painful sensation without any actual need - neuralgia nerve pain caused by nerve irritation. Each and every one is the result of a pain-staking research and development process. Customs restrictions and warranty issues prevent us from shipping to every international location. The herb’s active ingredients are harpagide and harpagoside, both iridoid glycosides with analgesic (pain-relieving) and anti-inflammatory actions. They enhance immunity and nervous system function. MS neuralgia pain falls into a few a categories: Devil’s claw is a South African herb with medicinally active roots. A: Yes. The ingredients which had caused effects closest complaints of their patients, are actually the ingredients best suited to relieve them. What's more, the enzyme may promote healing in muscles and connective tissues. Wide-Spectrum Relief With NO Negative herbs for nerve pain relief Side-Effects! "Okay" is just not good enough for us! 075 percent works best for peripheral nerve pain—such as that from diabetic nerve damage, HIV and pain following cancer surgery. Aconitum napellus HPUS, Agaricus muscarius HPUS, Baryta muriatica HPUS, Calendula officinalis HPUS, Carbo vegetabilis HPUS, Carduus marianus HPUS, Chamomilla HPUS, Dulcamara HPUS, Glonoinum HPUS, Hamamelis Virginiana HPUS, Lycopersicum esculentum HPUS, Magnesia phosphorica HPUS, Phosphorus HPUS, Plumbum metallicum HPUS, Rhus toxicodendron HPUS, Secale cornutum HPUS. The best tea for fat loss FDA has regulated homeopathic medicine since 1938 by means of natural remedies for psoriasis treatment the guidelines in the HPUS, as herbs for nerve pain relief per the how to get someone to stop smoking weed Federal Food, Drug, and Cosmetic Act. Capsaicin puts the heat in natural remedies for psoriasis treatment hot peppers. Creams containing . Bromelain may benefit people with arthritis and conditions associated with musculoskeletal tension (such as TMJ syndrome), as well as those suffering trauma-related inflammation. Subscribe to Mother Earth Living today to get inspired on the art of living wisely and living well. It is also available as tincture (use 1 teaspoon up herbs for nerve pain relief to three times daily) and tea. It is your responsibility to check with your Customs Office to see if your country permits the importation of our herbs for nerve pain relief products. If you are not sure, please contact the Customs Office in your country before ordering a product, as we are not responsible for such charges. As an OTC homeopathic medicine, this product has not been clinically researched and is not intended to replace any medications prescribed by your physician. Devil’s claw extract has been shown to reduce osteoarthritic hip or knee pain by 25 percent and improve mobility within a few weeks. They left nothing out. For more details please see our International Shipping Policy. However, all of our products sold internationally are meant for personal use - up to a 90 day supply of any one product may be purchased. Stroke survivors with CPSP may either experience numbness and be unable to detect touch or temperature changes, or experience extreme sensitivity which causes all stimuli to be interpreted as painful. Each issue of Mother Earth Living features advice to create naturally healthy and nontoxic homes for yourself and your loved ones. Welcome to Mother how do you feel when your blood sugar is high Earth Living, the authority on green lifestyle and design. Seeking alternatives to the often dangerous and even deadly how to know if you have liver problems treatments of their time, brave, pioneering homeopathic doctors would take small doses of natural ingredients to observe and note every effect the ingredient had.


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All about Multivitamins That You Need to Know
- September 28, 2018
- Health and Fitness
- No Comments
Basic multivitamins are a combination of vitamins A, B, C, D and E that you need to take regularly as part of your daily diet. Does your daily intake contain all of them or can you ensure taking them in these tough times when workloads can be punishing and when work hours are ungodly? Certain not, as many people coast to coast are realizing these days.
They are increasingly relying on supplements to provide them with the adequate intake of multivitamins and they are also available in the form of tablets. You can find nutritional and mineral vitamin supplements for all ages from www.bodybynaturesupplements.com/full-range-vegan-supplements-vitamins.
Individuals always ask what the best multivitamin is. Actually, there isn’t one. The purposes behind this are very basic. Exceptionally supplement works contrastingly and has diverse capacities with the human body. Saying that any of these are the best one isn’t just obscure, however conceivably unsafe for your wellbeing. You never need to take similar measurements of a similar supplement again and again.
To discover a multivitamin that you figure you will be OK with, either converse with your specialist or do some online research. In the event that you are in a school science class, you can ask your educator his assessment. Nutritionists are a decent asset too. You will be unable to discover a nutritionist out in the open, be that as it may, so have a go at discovering one on the web.
There are numerous discussions on multivitamin admission and which one to take for a particular issue with the body. Favorable circumstances of a discussion incorporate a mass measurement of individuals giving you their supposition on what you ought to do when taking vitamins. Inconveniences incorporate insignificance and the trickiness of the discussion individuals sentiments. As I would see it, I would avoid discussions except if the multivitamin you are searching for has no unsafe symptoms.
Another approach to discovering a multivitamin that fits you is to simply attempt a few and test their advantages. Focal points of this incorporate getting a good deal on the obtaining on supplements and time squandered in discovering them. Inconveniences incorporate symptoms from the multivitamins that you are trying. As I would like to think, don’t test a vitamin on the off chance that it has any negative outcomes.
Many of these supplements are not sticky or stretch like chewing gums, but they can be consumed in a whole form. It is better to take these vitamins with your meals as the iron content in them can cause stomach irritation. Multivitamins for women also contain a large number of nutrients that are women specific and tailored for their requirement.


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ZenMind offers a unique and natural way to promote relaxation without inducing drowsiness. This formula contains a combination of L-theanine and GABA, which have been shown to stimulation the production of alpha-wave activity in the brain. Alpha-waves play a role in reducing anxiety while increasing mental alertness and acuity.
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DHEA is an endogenous (made in the human body) hormone secreted by the adrenal gland. It serves as precursor to male and female sex hormones (androgens and estrogens). DHEA levels in the body peak at the age of 20 for both men and women. As the level of DHEA decreases, age-related illnesses increase.
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Unlike chemically-produced pharmaceutical sleep aids, melatonin is an all-natural solution to sleep problems that won't leave you feeling groggy the next morning. The sublingual form of melatonin quickly dissolves under your tongue to be utilized immediately by the body.
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This highly concentrated milk thistle extract possesses antioxidant and free radical scavenging activity to cleanse the liver, stimulate the production of new liver cells to replace older damaged ones, help the body detox excess estrogens, protect the liver from toxic chemicals and pollutants, and improve psoriasis and glutathione levels.
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5-HTP (5-hydroxytryptophan) is a precursor to serotonin. Serotonin is the neurotransmitter that tells your brain that you are satisfied and do not need to eat more. Serotonin deficiency contributes to weight gain, depression, sleeplessness, anxiety, inflammation, and joint pain, among other symptoms.
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People experiencing stress, whether it is just the daily grind, relationship problems, or a recent illness, often feel tired (especially in the morning), irritable and less able to cope with challenges at work and home.
Supporting the adrenal glands is the key to maintaining well-being during times of stress. The adrenals respond to every stress by producing and secreting hormones, such as cortisol and adrenaline.
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Our remarkable “C” U-Later Wrinkles Serum™ contains two active ingredients; 12% Ester-C®, an exclusive patented form of Vitamin C (ascorbyl palmitate) that is proven to stimulate the growth of new collagen, and helps to prevent premature aging & wrinkling of the skin. Gingko Biloba increases circulation, prevents capillary fragility & helps boost collagen.


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Your Obligatory Portion Of Protein
This review aims to find out what is SHprotein and if it is really worth your attention. So, let's start our research! According to the producer, SHprotein is an inevitable bodybuilding supplement for those who want to gain muscle mass or just to keep fit. Each person needs the definite amount of protein every day. It is well-known that you can get protein by eating certain food but if you want to get muscle mass you need enough protein per day which is really difficult to control. SHprotein claims to provide you with the necessary amount of protein in an easy and convenient way because it is unreal to cook something quickly after your workout to be able to eat your obligatory portion of protein.
It sounds very reasonable but what do you really get by taking this supplement?
Let's check what it can offer its customers by looking inside the bottle.
Now we are going to discover whether SHprotein is enough powerful to help you in growing muscle mass.
According to the label of the product the main active ingredient is Performance Protein proprietary blend. But we don't know the quantity of each ingredient because the percentage daily value hasn't been established by the manufacturer. This blend consists of Whey Protein Concentrate, Milk Protein Concentrate, Whey Protein Isolate, Natural Flavours, Cinnamon, Xantham Gum, Sucraiose. It also contains Calcium.
Now let's have a closer look at some ingredients which are included into the protein blend.
Both Whey Protein Concentrate and Whey Protein Isolate might have the same side effects. If you have a milk allergy you should definitely refuse taking this product. It can cause a lot of side effects if you take more than it is recommended, for example, such side effects as increased bowel movements, nausea, cramps, fatigue and headache. The problem is that we don't know exactly how much is there in SHProtein as the producer doesn't reveal the percentage of all ingredients.
They can have some effect on your immune system and might improve the nutrient content of the diet. But there is no evidence that can prove that both ingredients are really effective for the goals that the product claims to help you to achieve. Other ingredients can't be considered to be active that's why there is no need to consider all of them as natural flavours, for example, can't help you in your workouts as well as Cinnamon and other additional supplements.
It should be noted that the formula of the supplement doesn't differ from many other products with the same claims.
There is nothing revolutionary in this supplement. It can't be called a scam but it doesn't contain any new and interesting combination to make such claims as it does.
Before purchasing any supplement it is a good idea to look through the existing reviews of those customers who have already had such an opportunity to use this product. According to the reported side effects there are some that are possible if you take SHprotein and here we are going to look how unsafe your supplement's intake can be. First of all, you should remember that if you have an allergy to milk then you shouldn't take SHprotein for sure in order to avoid severe allergic reaction. Some users have admitted having cramps and bloating while taking SHprotein.
Here you have an opportunity to get acquainted with the results that the SHprotein customers have received in order to take your decision whether you need this supplement or not.
"I have used SHprotein for a short period of time and had to stop taking it due to an allergic reaction though I didn't have a milk allergy before. I haven't noticed any visible results during my intake and can't tell you if it works properly if you take it for a longer period of time but one thing I have understood about SHprotein for sure - it is not as safe as the manufacturer claims. You should consult your pharmacist before taking SHprotein!"
"SHprotein was the worst choice which didn't help me at all. I was taking it as it was recommended on the label of the bottle but I didn't manage to grow muscle mass after several months of using SHprotein. I think it is just a scam!"
"Don't buy SHprotein because it is not so cheap and it is not worth paying money because it is not as effective as the producer promises its users. Unfortunately, I can't say anything positive about SHprotein because I had some unwanted side effects like problems with my stomach, headache and allergy. Don't believe that it is completely safe! Get a consultation of your doctor before taking a decision to purchase SHprotein!"
If you want to try SHprotein in spite of this review you can visit the official website or to make your order at any other online store. It is available at Amazon.com. Its prices can vary from site to site. The pretty good price is about $40 but usually it costs more.
Having a strong wish to build attractive body is really a great goal but as you can see SHprotein isn't able to be enough powerful to fulfill its promises properly. There is no evidence that it is completely safe because there were some customers who reported having negative side effects after taking the supplement. Moreover, it is not very cheap. If to take into account that it includes the ingredients which are common for such bodybuilding supplements then it can be called even overpriced for making the claims it can't perform properly. It can't be recommended as an effective supplement which is worth spending your time and money because of many reasons which have already been mentioned. Moreover, it is always better to choose the supplement which has been clinically proven and researched in the laboratory conditions. There is no such evidence about SHprotein, that's why it can't be chosen as one of the best supplements in this sphere.